CN115281219B - Chinese herbal medicine baical skullcap root preparation for promoting growth of tomato plants and application method thereof - Google Patents
Chinese herbal medicine baical skullcap root preparation for promoting growth of tomato plants and application method thereof Download PDFInfo
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- CN115281219B CN115281219B CN202210971906.2A CN202210971906A CN115281219B CN 115281219 B CN115281219 B CN 115281219B CN 202210971906 A CN202210971906 A CN 202210971906A CN 115281219 B CN115281219 B CN 115281219B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/08—Magnoliopsida [dicotyledons]
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Abstract
The invention discloses a Chinese herbal medicine baical skullcap root preparation for promoting growth of tomato plants and a use method thereof, wherein the Chinese herbal medicine preparation comprises the following components: weighing appropriate amount of Scutellariae radix, placing into a glass container, adding 10 times of water, soaking for 1 hr, heating in 100deg.C water bath for 30 min, filtering with funnel and filter paper to collect medicinal liquid, adding equal amount of water into the residue, heating in 100deg.C water bath for 30 min, filtering with funnel and filter paper to collect medicinal liquid, mixing the medicinal liquids, and diluting to desired concentration until the concentration of Scutellariae radix extract is 6g/L. Based on the natural Chinese herbal medicine baical skullcap root preparation as the biological hormone, the tomato plant is treated by adopting a mode of combining spraying and root irrigation, so that the tomato plant growth promoting effect is achieved, the residues of heavy metals and pesticides of the tomato plant are avoided, and the safety is higher.
Description
Technical Field
The invention relates to the technical field of plant biostimulants, in particular to a Chinese herbal medicine baical skullcap root preparation for promoting growth of tomato plants and a use method thereof.
Background
Tomato, i.e. tomato, persimmon, belongs to a annual or perennial herb of the order of the tubular flower, the family of the Solanaceae, the genus Lycopersicon. The tomato fruits are rich in vitamins, dietary fibers, organic acids, carotenoids and other nutrient substances, are deeply favored by people, are vegetable crops with the widest cultivation and the largest consumption in the world, have continuously increased demand, realize the continuous targets of high yield, stable yield and high quality of tomatoes, and realize the great use of fertilizers and pesticides in a great way for improving the yield and quality of tomatoes.
In recent years, to obtain maximum yields, leading to a large and blind use of fertilizers and pesticides in agricultural production, a series of problems are posed: the ecological balance of farmland is damaged, the environment is seriously polluted, the health of people and livestock is endangered, and the plants are regulated by biological stimulation. The biological hormone is a novel product appearing in the agricultural market, mainly comprises seaweed extract, humic acid, chitin and derivatives thereof, microbial agents and the like, and has the effects of promoting crop growth, improving nutrient absorption and utilization efficiency and the like. At present, the biological stimulation hormone is gradually and widely applied to horticultural crops and grain crops, achieves the effects of increasing yield and income, provides assistance for the 'chemical pesticide fertilizer application reduction and synergy', and plays an important role in the rapid development of sustainable agricultural practice. Therefore, the development and application of the novel biostimulant have considerable prospect in the pesticide fertilizer market.
Chinese herbal medicine resources in China are rich, and recent researches show that secondary metabolic substances in the Chinese herbal medicine have various regulation and control effects on plant growth. The Chinese herbal medicine has the advantages of green, no pollution and environmental friendliness, and the natural Chinese herbal medicine is used as the raw material to prepare the novel plant biological hormone with the growth regulating activity, so that the novel plant biological hormone has an important role in the field of plant growth regulation. The Chinese herbal medicine extract is different from the conventional growth regulator, is environment-friendly and harmless to human and livestock. The baikal skullcap root is used as the traditional Chinese herbal medicine in China, belongs to the Labiatae plant, has wide pharmacological activity including various pharmacological effects such as anti-tumor, antioxidation, anti-inflammatory, antimicrobial, anti-aging and the like, is low in price and rich in resources, and is an excellent resource for developing novel biological stimulation hormone. Therefore, the Chinese herbal medicine with moderate price is selected, the Chinese herbal medicine is prepared into an environment-friendly Chinese herbal medicine preparation, and the Chinese herbal medicine preparation is directly applied to crops by utilizing the action of the plant regulation activity of the Chinese herbal medicine preparation, so that a new path is opened up for the development of natural green pollution-free biological stimulation.
Therefore, we propose a Chinese herbal medicine baical skullcap root preparation for promoting tomato plant growth and a using method thereof.
Disclosure of Invention
The invention mainly aims to provide a Chinese herbal medicine baical skullcap root preparation for promoting the growth of tomato plants and a use method thereof, which can effectively solve the problems in the background technology.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
in a first aspect, a Chinese herbal medicine preparation for promoting growth of tomato plants comprises a baical skullcap root preparation.
In a second aspect, the present invention also provides a method for preparing a Chinese herbal medicine preparation for promoting growth of tomato plants, comprising the following steps:
(1) Weighing appropriate amount of Scutellariae radix, placing into glass container, adding 10 times of water of Chinese herbal medicine into the container, and soaking for 1 hr;
(2) Heating in a water bath at 100deg.C for 30 min, filtering with a funnel and filter paper to collect medicinal liquid, adding equal amount of water into the residue, and heating in a water bath at 100deg.C for 30 min;
(3) Filtering with funnel and filter paper to collect medicinal liquid, mixing the medicinal liquid, and diluting to desired concentration until the concentration of Scutellariae radix extract is 6g/L.
Preferably, the water used in step 1 and step 2 is distilled water.
In a third aspect, the present invention provides a method of using a herbal formulation for promoting the growth of tomato plants comprising: the acting crop is tomato, and the acting part is leaf and root.
Compared with the prior art, the invention has the following beneficial effects: based on the natural Chinese herbal medicine baical skullcap root preparation as biological hormone, the tomato plant is treated by adopting a mode of combining spraying and root irrigation, so that the height of the tomato plant, the stem thickness, the root system size, the photosynthetic pigment content, the net photosynthetic rate, the stomatal conductance, the fluorescent parameters and the transpiration rate are increased, the accumulation of biomass is promoted, and the strong seedling index is promoted;
the formula of the invention is a pure traditional Chinese medicine formula, is natural, has small toxic and side effects, mild efficacy and no drug resistance, can be effectively absorbed by organisms, is not easy to cause soil environment pollution, is safe, pollution-free, residue-free, green, healthy and environment-friendly, and can effectively improve the planting benefit of tomatoes.
Drawings
FIG. 1 is a schematic diagram showing the effect of different treatment modes of the baical skullcap root preparation provided by the invention on the growth of tomato plant stems and leaves; wherein, figure a is the overall plant growth phenotype; b is plant height; c is the plant stem thickness; d is the area of the leaflet; CK represents leaf spraying water and root irrigation water; s represents spraying of the scutellaria baicalensis extract and root irrigation water on the leaves; r represents leaf spray water and root irrigation Scutellariae radix extract; SR means spraying the extract of Scutellaria baicalensis Georgi onto the leaves and irrigating the extract of Scutellaria baicalensis Georgi onto the roots;
FIG. 2 is a schematic diagram showing the effect of different treatment modes of the Baical skullcap root preparation on tomato leaf photosynthetic pigments; wherein, the graph A is the chlorophyll a content of the leaf; b is total chlorophyll content of the leaf; c is leaf carotenoid content; CK represents leaf spraying water and root irrigation water; s represents spraying of the scutellaria baicalensis extract and root irrigation water on the leaves; r represents leaf spray water and root irrigation Scutellariae radix extract; SR means spraying the extract of Scutellaria baicalensis Georgi onto the leaves and irrigating the extract of Scutellaria baicalensis Georgi onto the roots;
FIG. 3 is a schematic diagram showing the influence of different treatment modes of the Baical skullcap root preparation provided by the invention on the gas exchange parameters of tomato leaves; wherein, graph a is leaf net photosynthetic rate; b is a blade light system II; c is the air hole conductivity of the blade; d is the transpiration rate of the leaves; CK represents leaf spraying water and root irrigation water; s represents spraying of the scutellaria baicalensis extract and root irrigation water on the leaves; r represents leaf spray water and root irrigation Scutellariae radix extract; SR means spraying the extract of Scutellaria baicalensis Georgi onto the leaves and irrigating the extract of Scutellaria baicalensis Georgi onto the roots;
fig. 4 is a schematic diagram showing the influence of different treatment modes of the baikal skullcap root preparation provided by the invention on the root system size of tomatoes; wherein, figure a is tomato root phenotype; b is total root length; c is the root average diameter; d is root surface area; e is root volume; f is the number of root nodes; the number of G root tips; CK represents leaf spraying water and root irrigation water; s represents spraying of the scutellaria baicalensis extract and root irrigation water on the leaves; r represents leaf spray water and root irrigation Scutellariae radix extract; SR means spraying the extract of Scutellaria baicalensis Georgi onto the leaves and irrigating the extract of Scutellaria baicalensis Georgi onto the roots;
FIG. 5 is a schematic diagram showing the effect of different treatment modes of the Baical skullcap root preparation on tomato plant biomass; wherein, panel a is the biomass of the upper part of the tomato plant; b is biomass of underground parts of tomato plants; CK represents leaf spraying water and root irrigation water; s represents spraying of the scutellaria baicalensis extract and root irrigation water on the leaves; r represents leaf spray water and root irrigation Scutellariae radix extract; SR means spraying the extract of Scutellaria baicalensis Georgi onto the leaves and irrigating the extract of Scutellaria baicalensis Georgi onto the roots;
FIG. 6 is a schematic diagram showing the influence of different treatment modes of the baical skullcap root preparation provided by the invention on the strong seedling index of tomato plants; wherein, the graph A is the plant root-cap ratio; b is plant seedling strengthening index; CK represents leaf spraying water and root irrigation water; s represents spraying of the scutellaria baicalensis extract and root irrigation water on the leaves; r represents leaf spray water and root irrigation Scutellariae radix extract; SR means leaf spraying of the extract of Scutellaria baicalensis Georgi and root irrigation of the extract of Scutellaria baicalensis Georgi.
Detailed Description
The invention is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Examples
A Chinese herbal medicine preparation for promoting tomato plant growth comprises a baical skullcap root preparation.
The Chinese herbal medicine baical skullcap root preparation in the embodiment is obtained by the following steps:
(1) Weighing appropriate amount of Scutellariae radix, placing into glass container, adding 10 times of water of Chinese herbal medicine into the container, and soaking for 1 hr;
(2) Heating in a water bath at 100deg.C for 30 min, filtering with a funnel and filter paper to collect medicinal liquid, adding equal amount of water into the residue, and heating in a water bath at 100deg.C for 30 min;
(3) Filtering with funnel and filter paper to collect medicinal liquid, mixing the medicinal liquid, and diluting to desired concentration until the concentration of Scutellariae radix extract is 6g/L.
The application method of the Chinese herbal medicine preparation in the embodiment is as follows:
in the period of four leaves and one heart of the tomato, the tomato seedlings are treated in a spraying and root irrigation mode, and the treatment is carried out once every three days, and five times of treatment are carried out;
the acting crop is tomato, and the acting part is leaf and root.
Test example: the tomato variety tested was "Ailsa Craig".
Test grouping: accelerating germination of tomato seeds in a 28 ℃ incubator, sowing the tomato seeds in a plug tray filled with vermiculite after the seeds are exposed to white, and watering 1/2Hogland nutrient solution every day. When tomatoes grow to four leaves and one heart, selecting seedlings with consistent growth vigor, dividing the seedlings into two groups with similar growth vigor, transplanting the seedlings into 8 gamma 9cm small bowls with nutrient matrixes (turf: perlite: vermiculite=3:1:1), controlling the temperature to be about 25 ℃, controlling the humidity to be about 65%, illuminating for 16 hours and darkening for 8 hours. Treating in 4 groups, wherein the first group is blade spraying water and root irrigation water, and the CK is used for representing; the second group is to spray the baikal skullcap root preparation and root irrigation water for the leaves, which is denoted by S; the third group is to spray water on the leaves and irrigate the baical skullcap root preparation on the roots, and the third group is represented by R; the fourth group is to spray the preparation of Scutellaria baicalensis Georgi on leaves and irrigate the preparation of Scutellaria baicalensis Georgi on roots, expressed by SR, once every three days, for a total of 5 times.
The test mode is as follows: a completely randomized block design, 8 biological replicates were set.
Experimental results: referring to fig. 1, SR treatment significantly increased plant height, stem thickness and leaf area by 8.00%, 9.22% and 21.84% respectively, compared to the control; referring to fig. 2, the sr treated chlorophyll a content was increased by 5.23% compared to the control, and the difference was significant; the contents of chlorophyll and carotenoid in SR treatment are respectively improved by 1.99% and 1.81% compared with the control, but the difference is not obvious; referring to fig. 3, the net photosynthetic rate, leaf photosystem ii efficiency, stomatal conductance, and transpiration rate of tomato leaf were all highest under SR treatment, which were 38.11%, 39.14%, 37.07%, and 56.36%, respectively, higher than CK treatment, with significant differences; referring to fig. 4, the total root length, average root diameter, root surface area, root volume, root node number, and root tip number of the SR treated group were increased by 29.91%, 3.39%, 27.48%, 23.03%, 42.31%, and 22.78%, respectively, compared to CK, and the difference was significant, and the average root diameter of the SR treated group was increased by 3.39% compared to CK, but the difference was not significant; referring to FIG. 5, plant biomass was highest in SR treatment, and the difference was significant from CK treatment, with fresh and dry weight of the aerial parts under SR treatment increased by 10.70% and 26.65%, respectively; the fresh weight and dry weight of the subsurface fraction under SR treatment were increased by 32.00% and 28.66% respectively compared to CK treatment; referring to fig. 6, the tomato root cap ratio and the strong seedling index were both highest under SR treatment, 61.61% and 47.44% higher than CK treatment, respectively, and the differences were significant.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (3)
1. The application of the Chinese herbal medicine preparation in promoting the growth of tomato plants is characterized in that the Chinese herbal medicine preparation comprises a baical skullcap root preparation; the preparation method of the Chinese herbal medicine baical skullcap root preparation comprises the following steps:
(1) Weighing appropriate amount of Scutellariae radix, placing into glass container, adding 10 times of water of Chinese herbal medicine into the container, and soaking for 1 hr;
(2) Heating in a water bath at 100deg.C for 30 min, filtering with a funnel and filter paper to collect medicinal liquid, adding equal amount of water into the residue, and heating in a water bath at 100deg.C for 30 min;
(3) Filtering with funnel and filter paper to collect medicinal liquid, mixing the medicinal liquid, and diluting to desired concentration until the concentration of Scutellariae radix extract is 6g/L.
2. Use of the herbal formulation of scutellaria as defined in claim 1 for promoting the growth of tomato plants, wherein: in the period of four leaves and one heart of the tomato, the tomato seedlings are treated by spraying and root irrigation, and the treatment is carried out once every three days, and the total treatment is carried out five times.
3. The use of the herbal formulation of scutellaria baicalensis in promoting tomato plant growth according to claim 1, wherein: the action parts are blades and roots.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210971906.2A CN115281219B (en) | 2022-08-12 | 2022-08-12 | Chinese herbal medicine baical skullcap root preparation for promoting growth of tomato plants and application method thereof |
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| CN202210971906.2A CN115281219B (en) | 2022-08-12 | 2022-08-12 | Chinese herbal medicine baical skullcap root preparation for promoting growth of tomato plants and application method thereof |
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| CN115281219A CN115281219A (en) | 2022-11-04 |
| CN115281219B true CN115281219B (en) | 2023-06-30 |
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| CN1618285A (en) * | 2003-11-18 | 2005-05-25 | 河北宣化农药有限责任公司 | Appliution of scutellaria baicalensis extractive as agricultural sterilizing agent, and agricultural sterilizing agent composition contg. same |
| CN104664550A (en) * | 2013-11-26 | 2015-06-03 | 崔桂林 | Method of extracting biological fresh-keeping agent from scutellaria baicalensis and application of biological fresh-keeping agent in fresh keeping of cherry tomatoes |
| CN104002344B (en) * | 2014-06-17 | 2016-03-16 | 吉首大学 | A kind of Baical Skullcap root P.E natural bamboo preservative and preparation method thereof |
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