CN104002344B - A kind of Baical Skullcap root P.E natural bamboo preservative and preparation method thereof - Google Patents
A kind of Baical Skullcap root P.E natural bamboo preservative and preparation method thereof Download PDFInfo
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- XLTFNNCXVBYBSX-UHFFFAOYSA-N wogonin Chemical compound COC1=C(O)C=C(O)C(C(C=2)=O)=C1OC=2C1=CC=CC=C1 XLTFNNCXVBYBSX-UHFFFAOYSA-N 0.000 claims description 8
- LNOHXHDWGCMVCO-NTKSAMNMSA-N wogonin 7-O-beta-D-glucuronide Chemical compound C1=C(O)C(C(C=C(O2)C=3C=CC=CC=3)=O)=C2C(OC)=C1O[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LNOHXHDWGCMVCO-NTKSAMNMSA-N 0.000 claims description 8
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- Chemical And Physical Treatments For Wood And The Like (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of Baical Skullcap root P.E natural bamboo preservative and preparation method thereof, this bamboo wood preservative is made up by mass percentage of following raw material: Baical Skullcap root P.E: 2.5% ~ 10%, ethanol 5% ~ 30%, surplus is distilled water, and the mass percent sum of raw material is 100%.The preparation method of this bamboo wood preservative is: ethanol and distilled water are mixed into the ethanol water of 5% ~ 30% mass percent by certain mass ratio, add Baical Skullcap root P.E, the total mass ratio of the quality of Baical Skullcap root P.E and ethanol and distilled water is 0.25 ~ 1:10,20 ~ 60min is stirred at 20 ~ 60 DEG C in the airtight tank body of vacuum decompression, be cooled to normal temperature, obtain the bamboo wood antiseptic solution containing Baical Skullcap root P.E.A kind of Baical Skullcap root P.E natural bamboo preservative of the present invention has antibacterial, insect protected effect, to bamboo wood rotten fungi brown rot fungus (gloeophyllum trabeum) and whiterot fungi (Corilus versicolor Quel.), there is good inhibitory action, and be green material completely, preparing process is simple, can save energy consumption, the safety comparing environment with chemical preservative is very high.
Description
Technical field
The present invention relates to a kind of bamboo wood preservative and preparation thereof, be specifically related to a kind of bamboo wood preservative containing Baical Skullcap root P.E and preparation method thereof.
Background technology
The means of defence of bamboo wood and bamboo product is mainly divided into two large classes: one is Physical, as infrared heating method, steam method, baking, microwave method, supercritical ultrasonics technology etc.; Two is chemical method, is with chemical agent effectively to kill the insect had in bamboo wood and the wood decay fungi infected, but also bamboo wood can be protected no longer to be corroded by bacterium worm.The major advantage of Physical is that toxicity is low, and environmental pollution is little, but sterilization worm weak effect; Chemical method is the most frequently used method preventing bamboo wood rotten, use chemical agent favorable anti-corrosion effect, the term of validity is long, do not damage the physicochemical characteristics of bamboo wood, but shortcoming is preservative is producing and leakage in use procedure and oozing out, chemical agent easily produces toxicity and causes adverse effect to environment, and the health of harm humans.Because environmental problem more and more receives the concern of people, also become the key selecting preservative.
Because traditional preservatives exists many deficiencies, force people to study and find person poultry harmless, environmentally safe, only poisonous to microorganism new food preservative.In the nearlyer coming year, countries in the world are all in the research being devoted to new efficient, low toxicity, environmental type preservative.The methanolic extract process poplar of locust tree, sequoia sempervirens etc. such as university Kamdem as state in Michigan, United States, under the poplar after process is exposed to brown rot fungus, timber weight-loss ratio declines 95%; Yu Wenxi etc. find that the ligroin extraction of Martin's perfume, Albizzia kalkora etc. has good inhibitory action to wood decay fungi.The preservative extracted from natural plants is except can be toxic to bamboo wood rot fungi; itself has natural degradable; the environment of surrounding and people and animals are not polluted and poisoned; develop nontoxic, efficient, that there is natural anticorrosion performance plant chemical ingredient; for the anticorrosion research of bamboo wood; can environmental pollution be reduced, of far-reaching significance to protecting human ecological environment and making full use of existing resource.
The root of large-flowered skullcap is perennial Labiatae herbaceous plant, and its medicinal beginning of the root of large-flowered skullcap is loaded in Tang Materia Medica, has good purging intense heat and detonicating, an effect of the cool blood that stops blooding, antiabortive heat extraction, and being widely used in tcm prescription, is the important source material of Chinese medicine.Clinical pharmacology research finds, the principle active component of the root of large-flowered skullcap comprises the flavonoidss such as scutelloside, baicalein, wogonoside and wogonin.We are found by research, the flavonoids active component contained by the root of large-flowered skullcap, can the growth of the rotten fungi brown rot fungus (gloeophyllum trabeum) of permanently effective suppression bamboo wood and whiterot fungi (Corilus versicolor Quel.), for bamboo wood preservative treatment.
Summary of the invention
The object of the invention is to, a kind of bamboo wood preservative containing Baical Skullcap root P.E and preparation method thereof is provided, this bamboo wood preservative have nontoxic, be easily degraded, the good characteristic of environmentally safe, be desirable natural bamboo preservative that is efficient, green, environmental protection.
In order to realize above-mentioned task, the present invention adopts following technical solution:
Containing a bamboo wood preservative for Baical Skullcap root P.E, it is characterized in that, this bamboo wood preservative is made up by mass percentage of following raw material:
Baical Skullcap root P.E: 2.5% ~ 10%, ethanol 5% ~ 30%, surplus is distilled water, and the mass percent sum of raw material is 100%.
In described Baical Skullcap root P.E, in general flavone, mass percentage >=90%, is made up of following monomer component: baicalein: 90% ~ 99%, scutelloside: 0.1% ~ 2%, wogonin: 0.3% ~ 6%, wogonoside: 0.1% ~ 2% by mass percentage.
In the present invention, the preparation method of bamboo wood preservative carries out in the steps below:
Ethanol and distilled water are mixed into the ethanol water of 5% ~ 30% mass percent by certain mass ratio, add Baical Skullcap root P.E, the total mass ratio of the quality of Baical Skullcap root P.E and ethanol and distilled water is 0.25 ~ 1:10,20 ~ 60min is stirred at 20 ~ 60 DEG C in the airtight tank body of vacuum decompression, be cooled to normal temperature, obtain the bamboo wood antiseptic solution containing Baical Skullcap root P.E.
Preferred Baical Skullcap root P.E active constituent content is in general flavone, and mass percentage >=90%, is made up of following monomer component: baicalein: 95%, scutelloside: 1%, wogonin: 3%, wogonoside: 1% by mass percentage.
The bamboo wood preservative of above-mentioned preferred Baical Skullcap root P.E is adopted to prepare the application in the preparation suppressing brown rot fungus (gloeophyllum trabeum) and whiterot fungi (Corilus versicolor Quel.), when in bamboo wood preservative preparation, Baical Skullcap root P.E mass percent is 2.5%, Baical Skullcap root P.E to brown rot fungus (gloeophyllum trabeum) and whiterot fungi (Corilus versicolor Quel.) inhibition better, when in bamboo wood preservative preparation, Baical Skullcap root P.E mass percent is progressively increased to 10%, fungistatic effect increases significantly.
Adopt the bamboo wood preservative of above-mentioned preferred Baical Skullcap root P.E when bamboo wood preservative treatment uses, when Baical Skullcap root P.E mass percent is 10% in bamboo wood preservative preparation, reach best antiseptic effect.
The invention has the advantages that: adopt Baical Skullcap root P.E to be have antibacterial, insect protected effect as bamboo wood preservative, to bamboo wood rotten fungi brown rot fungus (gloeophyllum trabeum) and whiterot fungi (Corilus versicolor Quel.), there is good inhibitory action, and be green material completely, preparing process is simple, can save energy consumption, the safety comparing environment with chemical preservative is very high.
Embodiment
Embodiment 1: containing the preparation of the bamboo wood antiseptic solution of Baical Skullcap root P.E 2.5%
Containing a bamboo wood preservative for Baical Skullcap root P.E, it is characterized in that, this bamboo wood preservative is made up by mass percentage of following raw material:
Baical Skullcap root P.E: 2.5%, ethanol 30%, surplus is distilled water, and the mass percent sum of raw material is 100%.
In described Baical Skullcap root P.E, in general flavone, mass percentage >=90%, is made up of following monomer component: baicalein: 95%, scutelloside: 1%, wogonin: 3%, wogonoside: 1% by mass percentage.
In the present invention, the preparation method of bamboo wood preservative is as follows:
Ethanol and distilled water are mixed into the ethanol water of 30% mass percent by certain mass ratio, add Baical Skullcap root P.E, the total mass ratio of the quality of Baical Skullcap root P.E and ethanol and distilled water is 0.25:10,60min is stirred at 45 DEG C in the airtight tank body of vacuum decompression, be cooled to normal temperature, obtain the bamboo wood antiseptic solution containing Baical Skullcap root P.E.
Embodiment 2: containing the preparation of the bamboo wood antiseptic solution of Baical Skullcap root P.E 5%
Containing a bamboo wood preservative for Baical Skullcap root P.E, it is characterized in that, this bamboo wood preservative is made up by mass percentage of following raw material:
Baical Skullcap root P.E: 5%, ethanol 30%, surplus is distilled water, and the mass percent sum of raw material is 100%.
In described Baical Skullcap root P.E, in general flavone, mass percentage >=90%, is made up of following monomer component: baicalein: 95%, scutelloside: 1%, wogonin: 3%, wogonoside: 1% by mass percentage.
In the present invention, the preparation method of bamboo wood preservative is as follows:
Ethanol and distilled water are mixed into the ethanol water of 30% mass percent by certain mass ratio, add Baical Skullcap root P.E, the total mass ratio of the quality of Baical Skullcap root P.E and ethanol and distilled water is 0.5:10,60min is stirred at 45 DEG C in the airtight tank body of vacuum decompression, be cooled to normal temperature, obtain the bamboo wood antiseptic solution containing Baical Skullcap root P.E.
Embodiment 3: containing the preparation of the bamboo wood antiseptic solution of Baical Skullcap root P.E 10%
Containing a bamboo wood preservative for Baical Skullcap root P.E, it is characterized in that, this bamboo wood preservative is made up by mass percentage of following raw material:
Baical Skullcap root P.E: 10%, ethanol 30%, surplus is distilled water, and the mass percent sum of raw material is 100%.
In described Baical Skullcap root P.E, in general flavone, mass percentage >=90%, is made up of following monomer component: baicalein: 95%, scutelloside: 1%, wogonin: 3%, wogonoside: 1% by mass percentage.
In the present invention, the preparation method of bamboo wood preservative is as follows:
Ethanol and distilled water are mixed into the ethanol water of 30% mass percent by certain mass ratio, add Baical Skullcap root P.E, the total mass ratio of the quality of Baical Skullcap root P.E and ethanol and distilled water is 1:10,60min is stirred at 45 DEG C in the airtight tank body of vacuum decompression, be cooled to normal temperature, obtain the bamboo wood antiseptic solution containing Baical Skullcap root P.E.
Embodiment 4: the bamboo wood preservative bacteriostasis property containing Baical Skullcap root P.E is tested
The preparation of PDA medium: after potato is cleaned peeling, be cut into small pieces.Take potato fritter 200g, add water 1000ml, after boiling 20 minutes, filters (4 layers of gauze natural filtration).In filtered juice, supply moisture to 1000ml, in potato liquor, add 19g agar and 20g sucrose, boil, after making it dissolve, supply moisture to 1000ml, high pressure (gauge pressure 0.1MPa, temperature 121 DEG C, time 25min) after sterilizing, be divided in sterilized plate, be placed in workbench.
The process of filter paper: make with card punch the little scraps of paper that diameter is 5mm, put into plane ware, high pressure (gauge pressure 0.103MPa, temperature 121 DEG C, time 25min) sterilizing, taking-up cools, and obtains the aseptic little scraps of paper.
The preparation of antibacterial liquid: the bamboo wood preservative containing Baical Skullcap root P.E is for subsequent use after aseptic process.
The preparation of Corilus versicolor Quel., gloeophyllum trabeum suspension: Corilus versicolor Quel., gloeophyllum trabeum are inoculated in the potato culture after sterilizing, cultivate 2 to 3 days under room temperature, activate 3 times, getting well-grown third generation bacterium, in the 10ml physiological saline of sterilizing, be mixed with bacteria suspension for subsequent use.
Antibacterial assay method: on superclean bench, opens culture dish, pipettes bacteria suspension 1mL in media surface rapidly, and be coated with evenly with aseptic spreading rod with 1mL pipette.Sterilized filter paper is soaked 30min in antibacterial liquid simultaneously, taking-up is placed in fan blower is opened by superclean bench dries up, get with the tweezers of calcination the filter paper soaking liquid after cooling and be put in culture dish, light pressure, make the scraps of paper and medium close contact (each cultivation pastes 6 pieces containing same extract filter paper, each 3 of left and right).Culture dish is put into incubator to cultivate 2 to 3 days, measure the diameter of inhibition zone, calculating mean value, compares the size of inhibition zone, measures fungistatic effect.
Experimental result: adopt filter paper enzyme research containing the bamboo wood preservative of Baical Skullcap root P.E to the bacteriostasis property of whiterot fungi Corilus versicolor Quel. and brown rot fungus gloeophyllum trabeum.Result is: when (1) liquor strength is 2.5%, and Baical Skullcap root P.E is better to whiterot fungi Corilus versicolor Quel. inhibition, and liquor strength is progressively increased to 10%, and fungistatic effect increases significantly; (2) when liquor strength is 2.5%, Baical Skullcap root P.E is better to brown rot fungus gloeophyllum trabeum inhibition, and liquor strength is progressively increased to 10%, and fungistatic effect increases significantly.
Embodiment 5: the bamboo wood antiseptic property containing the bamboo wood preservative of Baical Skullcap root P.E is studied
On the basis containing the test of Baical Skullcap root P.E bamboo wood preservative bacteriostasis property, in order to show that bamboo wood preservative containing Baical Skullcap root P.E is to the antiseptic effect of bamboo wood further, according to " People's Republic of China's forestry industry standard--wood preservative is to rot fungi toxicity test room test method " (LY/T1283-1998), carry out the bamboo wood anti-corrosion performance test of Baical Skullcap root P.E.
Sample soaks liquid: before row note, be first placed in the air dry oven of 100 DEG C by each block bamboo wood sample (size 20mm*20mm*5mm) and dry to constant weight, be accurate to 0.01g.Bamboo wood sample after constant weight is put into respectively the bamboo wood preservative liquid of different Baical Skullcap root P.E concentration, in vacuum drying chamber, vacuum (0.01MPa) impregnates predetermined suction dose, absorb the supernatant liquid of every block specimen surface with filter paper after taking-up, weigh immediately, be accurate to 0.01g, obtain the sample weight after impregnating.By air-dry for the sample after soaking, constant weight.
The preparation of river sand sawdust medium: brown sugar, corn flour, populus ussuriensis sapwood sawdust (0.4 ~ 0.75mm), dry river sand (0.4 ~ 0.75mm) are in 1:8.5:15:150(weight) ratio mix thoroughly, pour in blake bottle, every bottle is 160g.Pave surface, put on its surface and raise wood, in bottle, slowly add 90ml Fructus Hordei Germinatus liquid glucose, fill in tampon and bag waterproof paper, in steam sterilization pan after sterilizing 1h, take out, be placed on superclean bench for subsequent use after cooling.
The inoculation of river sand sawdust medium and cultivation: on aseptic operating platform, get mycelia block and be placed on and raise on river sand sawdust medium that wood connects, plug tampon bag waterproof paper, is put in incubator, 29 DEG C of cultivations.Covered by mycelia completely until raise wood surface.
Bamboo wood sample multilayer after soaking is bound up with gauze, puts into steam sterilization pan, steam sterilizing 30min at 100 DEG C.After cooling, under sterile conditions, what sample is placed on northern mycelia in blake bottle covers raises on wood, and every block is raised on wood and put one piece of sample.Be stoppered tampon bag waterproof paper, put into incubator 29 DEG C and cultivate 12 weeks.
After rotten through 12 weeks, bamboo wood sample is taken out from blake bottle, carefully strike off mycelia and the impurity of specimen surface.Sample is put into air dry oven, at 100 DEG C, is dried to constant weight, weighs one by one, be accurate to 0.01g, the loss in weight after calculating sample is rotten, thus obtain the toxicity limit value of this preservative.
Experimental result: bamboo wood is III grade to whiterot fungi Corilus versicolor Quel. and the natural corrosion resistant grade of brown rot fungus gloeophyllum trabeum, is IV grade to the natural corrosion resistant grade of mould paecilomyces varioti.Adopt bamboo wood preservative containing Baical Skullcap root P.E to the suppression of whiterot fungi Corilus versicolor Quel. and brown rot fungus gloeophyllum trabeum, along with the increase of liquor strength and preservative maintenance dose, rate of weight loss reduces all gradually but does not present obvious gradient trend, concentration more than 4% can reach I level corrosion resistant grade, reaches best antiseptic effect when concentration is 10%.
Claims (3)
1. the bamboo wood preservative containing Baical Skullcap root P.E, it is characterized in that, this bamboo wood preservative is made up by mass percentage of following raw material: Baical Skullcap root P.E: 2.5% ~ 10%, ethanol 5% ~ 30%, surplus is distilled water, and the mass percent sum of raw material is 100%; In described Baical Skullcap root P.E, in general flavone, mass percentage >=90%, general flavone is made up by mass percentage of following monomer component: baicalein: 90% ~ 99%, scutelloside: 0.1% ~ 2%, wogonin: 0.3% ~ 6%, wogonoside: 0.1% ~ 2%.
2. the preparation method of a kind of bamboo wood preservative containing Baical Skullcap root P.E according to claim 1, it is characterized in that, carry out according to following steps: ethanol and distilled water are mixed into the ethanol water of 5% ~ 30% mass percent by certain mass ratio, add Baical Skullcap root P.E, the total mass ratio of the quality of Baical Skullcap root P.E and ethanol and distilled water is 0.25 ~ 1:10,20 ~ 60min is stirred at 20 ~ 60 DEG C in the airtight tank body of vacuum decompression, be cooled to normal temperature, obtain the bamboo wood antiseptic solution containing Baical Skullcap root P.E.
3. a kind of bamboo wood preservative containing Baical Skullcap root P.E according to claim 1, it is characterized in that, in described Baical Skullcap root P.E, in general flavone, mass percentage >=90%, general flavone is made up by mass percentage of following monomer component: baicalein: 95%, scutelloside: 1%, wogonin: 3%, wogonoside: 1%.
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| CN104626309B (en) * | 2015-01-23 | 2016-08-24 | 浙江三箭工贸有限公司 | A kind of bamboo softens modified technique |
| CN109725081A (en) * | 2019-01-09 | 2019-05-07 | 广东省测试分析研究所（中国广州分析测试中心） | Whether the discrimination method of Baical Skullcap root P.E is added in a kind of skin-care cosmetics |
| CN111844308A (en) * | 2020-06-05 | 2020-10-30 | 长宁县大旗竹业有限公司 | Bamboo preservative and preparation method and application thereof |
| CN113616801A (en) * | 2021-08-10 | 2021-11-09 | 南京益唯森生物科技有限公司 | Preparation method and application of antiseptic and antibacterial preparation containing Scutellariae radix extract |
| CN115281219B (en) * | 2022-08-12 | 2023-06-30 | 山东农业大学 | Chinese herbal medicine baical skullcap root preparation for promoting growth of tomato plants and application method thereof |
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Application publication date: 20140827 Assignee: Xiangxi Hongcheng Pharmaceutical Co.,Ltd. Assignor: JISHOU University Contract record no.: X2023980043276 Denomination of invention: A natural bamboo preservative extracted from Scutellaria baicalensis and its preparation method Granted publication date: 20160316 License type: Exclusive License Record date: 20231016 |
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