CN101120678B - Gummosis protective therapeutic agent and its preparation method - Google Patents
Gummosis protective therapeutic agent and its preparation method Download PDFInfo
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- CN101120678B CN101120678B CN2007101137548A CN200710113754A CN101120678B CN 101120678 B CN101120678 B CN 101120678B CN 2007101137548 A CN2007101137548 A CN 2007101137548A CN 200710113754 A CN200710113754 A CN 200710113754A CN 101120678 B CN101120678 B CN 101120678B
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Abstract
The present invention discloses a gummosis protection therapeutic agent and a preparation method of the gummosis protection therapeutic agent, the weight percentages of the raw materials of which are 40 to 50 percent of 27 percent lipid film emulsifying agent, 0.4 to 0.6 percent of 40 percent flusilazole emulsifiable concentrate, 1.9 to 2.1 percent of additive, 0.1 to 0.3 percent of 20 percent naphthylacetic acid powder agent and 47.4 to 57.4 percent of 1 percent gentian violet. The preparation method of the present invention is to uniformly heat and mix the 27 percent lipid film emulsifying agent with the 40 percent flusilazole emulsifiable concentrate and the additive in the water bath under 60 centigrade degrees to be made in to a mixed solution A; additionally to uniformly heat and mix the 20 percent naphthylacetic acid with the gentian violet in the water bath under 60 centigrade degrees until the 20 percent naphthylacetic acid is completely dissolved to be made into a mixed solution B; then to gradually pour the mixed solution A into the mixed solution B and to mix the solutions while pouring until the solution is uniformly mixed without layer division. The present invention has dual function effects of physically screening and preventing the disease and penetrating to sterilize and to cure the disease, which has high cure rate, long medicinal effective period and obvious prevention effect.
Description
Technical field
The present invention relates to a kind of protection treatment bactericidal agent and compound method thereof of preventing and treating fruit tree gummosis, belong to the fruit-bearing forest technical field of pesticide.
Background technology
Gummosis is the serious plant disease that generally takes place on the tone fruit trees such as peach, almond, cherry, apricot, and the limb of mainly causing harm is injured heavier with trunk and 3 years above boughs of life.The limb gummosis mainly is that pathogen infections such as grape seat chamber bacterium cause.Cause of disease borrows wind, rain to propagate with conidium, and wound and hole skin are the main paties of invading.Near their early stage water stain shape occurs or swells slightly hole skin verruca, big and the protuberance of flaring subsequently, circular or irregular shape, about 5～the 20mm of diameter, later stage " bubble shape " protuberance cracking overflows resin, just be colourless translucent thin and sticking flexible glue, soon become dark brown, the quality hardening is crystalloid, and expanding in the suction back becomes the colloid of peptone shape.Cut the blister spot open, visible cortex variable color necrosis is full of the thickness glue under the cortex, and the xylem surface is the browning look also.Last sick portion drying shrinkage depression, cortex cracking form circular or irregular shape scab, sometimes the little black grain point of scattered syringe needle shape on the scab.The blackening of killed branch rough surface, serious causes limb withered, and tree vigo(u)r is weak, and output falls sharply, even the death of tree body, becomes the big obstacle in the planting fruit trees industry.
In the management of the orchard of current routine; mainly to strike off focus, coating protection, institute's with medicament mainly contains the beautiful god of good fortune, lime sulfur, pesticide corrosion 120, copper sulphate etc., though play a role to the control of gummosis; but there are more problems and shortcomings; show that mainly blindly medication, the mode of action are single, control efficiency instability, aspect such as the drug effect phase is short; cause the general control effect undesirable; scab recurrence rate height, wound healing is poor, causes vicious circle.
Summary of the invention
Single for solving the current mode of action that exists in the gummosis technology of preventing and treating, the control efficiency instability, problems such as drug effect phase weak point the invention provides a kind of gummosis protective therapeutic agent and preparation method thereof, and its technical scheme of taking is:
The percentage by weight of this each raw material components of protection therapeutic agent is: 27% high fat film emulsion 40-50%, 40% Flusilazole missible oil 0.4-0.6%, auxiliary agent 1.9-2.1%, 20% methyl pulvis 0.1-0.3%, 1% gentian violet 47.4-57.4%.
The optimum weight percentage of each raw material components is: .27% high fat film emulsion 45%, 40% Flusilazole missible oil 0.5%, auxiliary agent 2%, 20% methyl pulvis 0.1%, 1% gentian violet 52.4%.
The preparation method of this protection therapeutic agent is: with 27% high fat film emulsion and 40% Flusilazole missible oil and auxiliary agent heated and stirred, mixing in 60 ℃ of water-baths, be mixed with the A mixed liquor; Till in addition heated and stirred mixing to 20% methyl dissolves fully in 60 ℃ of water-baths with 20% methyl and gentian violet, be mixed with the B mixed liquor; The A mixed liquor is slowly poured in the B mixed liquor, and the limit bevelling stirs, to mixing not stratified till.
In the above-mentioned raw materials component, high fat film is made up of higher aliphatic, executes on the fruit tree surface and can diffuse to form one deck monomolecular film automatically, prevents infecting and the germ expansion of outside disease, has ventilative printing opacity, isolates diseases prevention, seals water conservation, medicament synergy characteristics; Flusilazole belongs to novel triazole bactericidal agent, has bactericidal activity height, the wide characteristics of fungicidal spectrum; Gentian violet belongs to basic-dyeable fibre class Cidex-7 thing, has the characteristics of surface disinfection sterilization, convergence wound; Methyl is a kind of high activity auxins plant growth regulator, has activating cell, is beneficial to the characteristics that callus forms, promotes the scab healing.
The present invention is a carrier with efficient film forming agent; with surface sterilization, the growth of infiltration sterilization, cell activation, seal various technology such as water conservation and combine together; very fast formation adipose membrane protective layer after this product is smeared; resistance of rainwater washing against has osmosis strongly, cause of disease invasion both capable of blocking; kill and suppress the cause of disease growth; can seal water conservation fast again, activating cell promotes wound healing.Therefore the present invention has the physical isolation diseases prevention and permeates the double effects that sterilization is cured the disease, the cure rate height, and the drug effect phase is long, and control efficiency is obvious.
Embodiment
Below in conjunction with embodiment the present invention is further described:
Embodiment 1:
At first take by weighing each raw material, 27% high fat film emulsion 45g during every 100g comprises, 40% Flusilazole missible oil 0.5g, auxiliary agent 2g, 20% methyl pulvis 0.1g, 1% gentian violet 52.4g according to the shared percentage by weight of each raw material components.
With 27% high fat film emulsion and 40% Flusilazole missible oil and auxiliary agent heated and stirred, mixing in 60 ℃ of water-baths, be mixed with the A mixed liquor subsequently; Till in addition heated and stirred mixing to 20% methyl dissolves fully in 60 ℃ of water-baths with 20% methyl and gentian violet, be mixed with the B mixed liquor; The A mixed liquor is slowly poured in the B mixed liquor, and the limit bevelling stirs, to mixing not stratified till, both obtained this protection therapeutic agent.
Embodiment 2:
At first take by weighing each raw material, 27% high fat film emulsion 40g during every 100g comprises, 40% Flusilazole missible oil 0.6g, auxiliary agent 1.9g, 20% methyl pulvis 0.3g, 1% gentian violet 57.2g according to the shared percentage by weight of each raw material components.
With 27% high fat film emulsion and 40% Flusilazole missible oil and auxiliary agent heated and stirred, mixing in 60 ℃ of water-baths, be mixed with the A mixed liquor subsequently; Till in addition heated and stirred mixing to 20% methyl dissolves fully in 60 ℃ of water-baths with 20% methyl and gentian violet, be mixed with the B mixed liquor; The A mixed liquor is slowly poured in the B mixed liquor, and the limit bevelling stirs, to mixing not stratified till, both obtained this protection therapeutic agent.
Embodiment 3:
At first take by weighing each raw material, 27% high fat film emulsion 49.9g during every 100g comprises, 40% Flusilazole missible oil 0.4g, auxiliary agent 2.1g, 20% methyl pulvis 0.2g, 1% gentian violet 47.4g according to the shared percentage by weight of each raw material components.
With 27% high fat film emulsion and 40% Flusilazole missible oil and auxiliary agent heated and stirred, mixing in 60 ℃ of water-baths, be mixed with the A mixed liquor subsequently; Till in addition heated and stirred mixing to 20% methyl dissolves fully in 60 ℃ of water-baths with 20% methyl and gentian violet, be mixed with the B mixed liquor; The A mixed liquor is slowly poured in the B mixed liquor, and the limit bevelling stirs, to mixing not stratified till, both obtained this protection therapeutic agent.
When using this medicament, at first remove and remove fruit tree disease portion dead skin, breach and blob of viscose, draw in length and breadth at sick cortex place with scraper, dip in scrub-brush then and get this medicament brushing, scope slightly exceeds disease portion, firmly, makes this medicament can infiltrate sick portion during brush, after treating that soup is slightly done, skin is coated with one deck again and seals water-loss reducer.
Toxicity Determination:
The toxicity test method adopts growth rate method.Every kind of medicament established 7 concentration gradients by a times interpretation of the law, under aseptic technique, the medicament (concentration ratio is high 50 times for surveying concentration) of the above-mentioned different gradient concentrations of 1mm is evenly mixed with 49ml PSA medium respectively, pouring 4 diameters into is in the sterile petri dish of 9cm, after solidifying, and every ware inoculation diameter 0.5cm pathogenic bacteria (pure culture) (grape seat chamber bacterium), 25 ± 1 ℃ of constant temperatures were cultivated 48,72 hours down, measuring colony diameter, is contrast with what add sterile water, calculates bacteriostasis rate.
Statistical method: the logarithm value with drug concentration (mg/Kg) is an independent variable, and the probit value of bacteriostasis rate is a dependent variable, sets up virulence regression equation, obtains the ED (EC50) of each medicament.
Table 1: gummosis protective therapeutic agent is to gummosis germ (grape seat chamber bacterium) toxicity test result
| Medicament | Virulence regression equation | r | EC 50(mg/Kg ) |
| 40% Flusilazole missible oil | y＝5.5757+0.7714x y＝5.4607+0.5492x | 0.9642 0.9766 | 0.1794 0.1450 |
| Gummosis protective therapeutic agent | y＝5.7435+0.6489x y＝5.5608+0.6605x | 0.9919 0.9883 | 0.0715 0.1035 |
| Would rather Wettable Powder more than 77% | y＝4.0319+0.7791x y＝1.7673+1.3336x | 0.9716 0.9405 | 17.4840 265.5452 |
| 4% pesticide corrosion 120 aqua | y＝3.1708+1.9035x y＝3.7857+0.8073x | 0.9843 0.9970 | 9.1398 31.9177 |
| 45% lime sulfur wetting powder | y＝3.1013+1.1816x y＝2.3576+1.2233x | 0.9678 0.9539 | 40.4517 144.6017 |
| 40% asomate wetting powder | y＝4.0808+1.3618x y＝3.4407+1.6164x | 0.9999 0.9996 | 4.7316 9.2179 |
Annotate: measure 25 ± 1 ℃ of temperature, last hurdle is 48 hours measurement results, and following hurdle is 72 hours results
Toxicity test the results are shown in Table 1.Gummosis protective therapeutic agent EC
50Minimum, indoor bacteriostatic activity is the highest, bactericide such as copper agent commonly used in the current production (more than 77% would rather Wettable Powder), pesticide corrosion 120, lime sulfur, asomate; And the gummosis protective therapeutic agent bacteriostatic activity also is higher than Flusilazole, shows that this prescription has synergistic effect.
Field gummosis therapeutic test:
Test is carried out in Tai Ningyang magnetic kiln almond base, and test tree is 4 years dense Pa Er of lifes (Nonpareil), chooses tree vigo(u)r is suitable, the state of an illness is close, control measures are consistent plant as trying material.Designing 6 tests handles: (1) gummosis protective therapeutic agent; 50 times of liquid of (2) 40% asomate wetting powders+80 times of liquid of 95% machine oil emulsion; (3) 5 Baume degrees lime sulfurs; 100 times of liquid of (4) 4% pesticide corrosion 120 aquas; Would rather 50 times of liquid of Wettable Powder more than (5) 77%; (6) blank is only removed colloid without chemicals treatment.Per 4 strains tree is a sub-district, repeats randomized arrangement 3 times.
Remove prevent or cure a disease portion's dead skin, breach and blob of viscose on April 17th, 2006 (before beginning rainy season), draw in length and breadth at sick cortex place with scraper, dip in scrub-brush then and get the paint brushing, scope slightly exceeds disease portion, exert oneself during brush, make medicament can infiltrate sick portion, treat that soup is slightly done after, skin is coated with one deck again and seals water-loss reducer.Be coated with once again in 7 days at interval.
Every sub-district is coated with 20 gummosis scabs (area, position close), and every processing amounts to 60.Be coated with back 60 days and observe cure (no longer gummosis) number and gummosis place healing state (stop gummosis, extremely organize to be replaced) on every side by cambium, with cure rate as control efficiency.
Table 2 field gummosis therapeutic test result
| Handle | The processing gummosis is counted | Cure number | Cure rate (%) | The significance of difference |
| How peaceful 50 times of 100 times of liquid of 80 times of 5 Baume degrees lime sulfur of 50 times+machine oil emulsion of gummosis protective therapeutic agent asomate, 4% pesticide corrosion 120 aqua | 60 60 60 60 60 | 56 49 40 44 35 | 93.3 81.7 66.7 73.3 58.3 | aA bAB bcBC bB cC |
| Contrast | 60 | 5 | 8.3 | dD |
By table 2 as seen, the gummosis protective therapeutic agent field control effect is best, and cure rate significantly be better than other each conventional treatment, and wound healing is fast more than 90.0%, and is smooth smooth.
The field demonstration:
On the basis of test in 2006, we carried out demonstration test at orchard, Qi man beach, Tai'an, Shandong application gummosis protective therapeutic agent in 2007, and seeds are respectively fruit trees such as peach, almond, apricot.
Using method: on April 14th, 2007 affected area blob of viscose and tertia are removed to exposing neocortex, drawn in length and breadth at sick cortex place with scraper, dip in scrubbing brush then and get gummosis protective therapeutic agent and firmly smear, scope slightly exceeds disease portion.After treating that soup is slightly done, skin is coated with one deck again and seals water-loss reducer.Be coated with once again in 7 days at interval.Every 100g gummosis protective therapeutic agent can be coated with gummosis scab 100-200 about diameter 10cm.
On June 20th, 2007, gummosis was contained the phase of sending out investigation; the average cure rate of gummosis protective therapeutic agent control demonstration area gummosis is more than 90.0%-95.0%; effect is significantly better than Routine Management district (average cure rate is at 70.0%-80.0%); and recurrence rate is low; wound healing is good, smooth in appearance is smooth, has obtained desirable control efficiency.
Claims (3)
1. protection therapeutic agent of preventing and treating fruit tree gummosis; the percentage by weight that it is characterized in that each raw material components is: 27% high fat film emulsion 40-50%, 40% Flusilazole missible oil 0.4-0.6%, auxiliary agent 1.9-2.1%; 20% methyl pulvis 0.1-0.3%, 1% gentian violet 47.4-57.4%.
2. the protection therapeutic agent of control fruit tree gummosis according to claim 1; the percentage by weight that it is characterized in that each raw material components is: 27% high fat film emulsion, 45%, 40% Flusilazole missible oil 0.5%, auxiliary agent 2%; 20% methyl pulvis, 0.1%, 1% gentian violet 52.4%.
3. the preparation method of the protection therapeutic agent of the described control fruit tree gummosis of claim 1, it is characterized in that, take by weighing each raw material according to the shared percentage by weight of each raw material components: 27% high fat film emulsion 40-50%, 40% Flusilazole missible oil 0.4-0.6%, auxiliary agent 1.9-2.1%, 20% methyl pulvis 0.1-0.3%, 1% gentian violet 47.4-57.4%; With 27% high fat film emulsion and 40% Flusilazole missible oil and auxiliary agent heated and stirred, mixing in 60 ℃ of water-baths, be mixed with the A mixed liquor; Till in addition heated and stirred mixing to 20% methyl dissolves fully in 60 ℃ of water-baths with 20% methyl and 1% gentian violet, be mixed with the B mixed liquor; The A mixed liquor is slowly poured in the B mixed liquor, and the limit bevelling stirs, to mixing not stratified till.
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| CN104782684B (en) * | 2015-04-30 | 2017-11-28 | 宜宾云辰乔木园林有限责任公司 | A kind of trees Wound protective agent and its preparation, application method |
| CN108294010A (en) * | 2018-04-10 | 2018-07-20 | 山东棉花研究中心 | A kind of medicament composition of containing trifloxystrobin, pesticide and its application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1157099A (en) * | 1996-02-13 | 1997-08-20 | 畅鹏举 | Preventing agent for fruit tree rot and its preparation method |
| CN1792150A (en) * | 2005-12-27 | 2006-06-28 | 云南农业大学 | High lipid membrane phytoncide contg. |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1157099A (en) * | 1996-02-13 | 1997-08-20 | 畅鹏举 | Preventing agent for fruit tree rot and its preparation method |
| CN1792150A (en) * | 2005-12-27 | 2006-06-28 | 云南农业大学 | High lipid membrane phytoncide contg. |
Non-Patent Citations (4)
| Title |
|---|
| 张冰，王宪德.甜樱桃树流胶病防治试验.落叶果树 5.2003,(5),58-59. |
| 张冰,王宪德.甜樱桃树流胶病防治试验.落叶果树 5.2003,(5),58-59. * |
| 李树流胶病防治技术研究初报.刘琪等.中国南方果树32 3.2003,32(3),67-69. |
| 李树流胶病防治技术研究初报.刘琪等.中国南方果树32 3.2003,32(3),67-69. * |
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