CN103601844A - Novel animal specimen preservation gel as well as preparation method and use thereof - Google Patents
Novel animal specimen preservation gel as well as preparation method and use thereof Download PDFInfo
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- CN103601844A CN103601844A CN201310567766.3A CN201310567766A CN103601844A CN 103601844 A CN103601844 A CN 103601844A CN 201310567766 A CN201310567766 A CN 201310567766A CN 103601844 A CN103601844 A CN 103601844A
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Abstract
The invention relates to a novel animal specimen preservation gel as well as a preparation method and use thereof and belongs to the field of environment-friendly novel materials. The gel is prepared from the raw materials in percentage by mass: 1-10% of acrylamide compound, 15-30% of Tris-HCl, 0.1-0.5% of N,N-methylene-bis-acrylamide, 5-20% of sodium lauryl sulfate, 0.5-1.0% of ammonium persulfate and 38.5-78.5% of solution which is prepared from water and has the pH of 3.5-5.0. The pH is 3.5-5.0, so that animals and plants are effectively inhibited from breeding bacteria and molds; the animal specimen preservation gel disclosed by the invention has good effects on preservation, fixation, sterilization and corrosion prevention; the preservation gel is simple and convenient to prepare, the raw materials are all low-toxicity environment-friendly chemicals, and the injury/harm to the human/environment is little, so that the gel is a very good product for producing an animal/plant specimen and is convenient to transport.
Description
Technical field
The present invention relates to a kind of novel zoological specimens and preserve gel, belong to environmental friendly material field.
Technical background
As everyone knows, sample is kept in transparent liquid, sample is kept intact as far as possible, be conducive to viewing and admiring of scientific research, teaching and people, current used liquid is mainly formaldehyde solution and has the allotment of volatile alcohol solution and form, but formaldehyde has strong impulse smell, in making and use procedure, not only contaminate environment, the more important thing is and be detrimental to health, also comparatively inconvenience while transporting in addition.
Ethanol medically can be used as sterilizing agent and sterilant, be subject to the inspiration of this traditional method, develop in recent years and using the preservation liquid for animal body specimen of ethanol as effective constituent, as Chinese patent CN88105221A discloses a kind of biological sample, protect look sanitas, it is that the materials such as ethanol, potassium acetate, sodium sulfate, glycerol, water are mixed with to mixed solution in proportion.But yet this preservation liquid life-time service animal proteinum hardening distortion.
Summary of the invention
The object of the present invention is to provide a kind of zoological specimens that do not contain formaldehyde or alcohols to preserve gel.Another object of the present invention is to provide a kind of method of preserving zoological specimens better effects if.
The inventor is surprised to find that: by selecting suitable component proportion can make a kind of transparent gel, animal is sealed up for safekeeping, not only overcome the above-mentioned shortcoming causing of formaldehyde and alcohols specimen preserving liquid, and there is better preservation effect, and this composition final state is solid gel, well preserved moisture and the state of sample, made again transportation become simple and convenient.
The preparation method that a kind of novel zoological specimens provided by the present invention are preserved gel, its desired raw material component and quality proportioning are as follows:
The preparation method that described novel zoological specimens are preserved gel is:
By acrylamide, Tris-HCl, N, the PH that N-methylene-bisacrylamide, sodium lauryl sulphate and water form is that 3.5-5.0 solution prepares and puts in previously prepd sample bottle, add ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel.Obtain novel zoological specimens and preserve gel.
The simultaneously claimed novel zoological specimens that utilize present method and make of the present invention are preserved gel.
In actual use, by acrylamide, Tris-HCl, N, the PH that N-methylene-bisacrylamide, sodium lauryl sulphate and water form is that 3.5-5.0 solution prepares and puts in previously prepd sample bottle, by animal to be preserved or careful the putting into wherein of carcase, add ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel again.
Preferably, carcase is cooked sterilization pretreatment, is expected to obtain better effect.
The gel that adopts above-mentioned technique means and form is to the figurate effect of animal tool, and compared to liquid preservative solution, gel is as clear as crystal, has no irritating odor, and transports also very convenient.The solution producing in process for preparation be PH be 3.5-5.0 acidic solution, there is sterilization, rot-resistant effect.Facts have proved, preserve after 2 years, the state of zoological specimens (color and luster, shape etc.) does not all change.
The present invention need not add any sanitas, because this gel self is just isolated by materials such as oxygen, and the gel ultimate constituent is polymer inorganic polymer, and PH 3.5-5.0 has effectively suppressed growing of animals and plants bacterium and mould.
Zoological specimens of the present invention preserve gel and have that preparation is simple, raw material is easy to get, to advantages such as human body and environment have no side effect, can be widely used in preparation of specimen's work in the scientific popularization places such as museum, school.Also the urgent preservation that can be applicable to zoologist's field acquisition sample, has broad application prospects and social value.
Accompanying drawing explanation
The present invention has accompanying drawing 4 width, for obtained the photo of sample according to embodiment 1.Wherein:
Fig. 1 is upright front view I;
Fig. 2 is upright front view II;
Fig. 3 is upright side-view I;
Fig. 4 is upright side-view II.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but these embodiment do not limit the scope of the invention.
Embodiment 1
The acrylamide compound that is 1%% by mass ratio, Tris-HCl of 30%, 0.1% N, the solution that the PH that N-methylene-bisacrylamide, 20% sodium lauryl sulphate and 38.5% water form is 5.0 prepares to be put in previously prepd sample bottle, small goldfish corpse is put into and wherein added 0.5% ammonium persulphate to wait for 3 minutes, and solution solidifies becomes gel.Obtain small goldfish gel sample.
Sample was observed in 1 month, 3 months, 6 months, 12 months, 24 months after sample preparation, finds that state, color and luster, the shape of small goldfish all changes without obvious respectively.
Latter 24th month of sample preparation, small goldfish taking-up is rinsed to the rear observation of taking out, the state of discovery small goldfish, color and luster, shape, all without any change, are placed in normal indoor environment afterwards, within 3 days, observe afterwards discovery small goldfish and have just started to decay rotten.
Embodiment 2
The acrylamide compound that is 10% by mass ratio, Tris-HCl of 15%, 0.5% N, the solution that the PH that N-methylene-bisacrylamide, 5% sodium lauryl sulphate and 78.5% water form is 3.5 prepares to be put in previously prepd sample bottle, little conch corpse is put into and wherein added 1.0% ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel after 2 years, and little conch state, pool become, shape does not all change.
Little conch sample was observed in 1 month, 3 months, 6 months, 12 months, 24 months after sample preparation respectively, finds that the state, color and luster, shape of little conch are all without any change.After sample preparation 24th month, little conch is taken out after rinsing and takes out and observe, find that the state, color and luster, shape of little conch, all without any change, are placed in normal indoor environment afterwards, 3 days afterwards the little conch of observation discovery start to decay rotten.
Zoological specimens of the present invention are preserved gel and are had good preservation, fixing, sterilization, preservative activity.And inferior preservation gel making is simple and convenient and raw material is low toxic and environment-friendly chemical, very little to human body and environmental hazard, be to make the good product of animal or plant specimen and be convenient to transportation.Can be widely used in preparation of specimen's work in the scientific popularization places such as museum, school.Also the urgent preservation that can be applicable to zoologist's field acquisition sample, has broad application prospects and social value.
Claims (3)
1. novel zoological specimens are preserved a preparation method for gel, and its desired raw material component and quality proportioning are as follows:
Its preparation process is:
By acrylamide, Tris-HCl, N, the PH that N-methylene-bisacrylamide, sodium lauryl sulphate and water form is that 3.5-5.0 solution prepares and puts in previously prepd sample bottle, add ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel, obtains novel zoological specimens and preserves gel.
2. novel zoological specimens are preserved a gel, it is characterized in that: be to prepare in accordance with the method for claim 1 and obtain.
3. novel zoological specimens are preserved a purposes for gel, are the making for zoological specimens, and its desired raw material component and quality proportioning are as follows:
By acrylamide, Tris-HCl, N, the PH that N-methylene-bisacrylamide, sodium lauryl sulphate and water form is that 3.5-5.0 solution prepares and puts in previously prepd sample bottle, by careful the putting into wherein of carcase to be preserved, add ammonium persulphate to wait for 3-5 minutes, solution solidifies becomes gel again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310567766.3A CN103601844B (en) | 2013-11-14 | 2013-11-14 | A kind of zoological specimens preserve gel and its production and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310567766.3A CN103601844B (en) | 2013-11-14 | 2013-11-14 | A kind of zoological specimens preserve gel and its production and use |
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| Publication Number | Publication Date |
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| CN103601844A true CN103601844A (en) | 2014-02-26 |
| CN103601844B CN103601844B (en) | 2016-02-10 |
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| CN201310567766.3A Expired - Fee Related CN103601844B (en) | 2013-11-14 | 2013-11-14 | A kind of zoological specimens preserve gel and its production and use |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042622A (en) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | A kind of guarantor's color fixing agent and preparation method thereof and the application in prepared by sample |
| CN109166439A (en) * | 2018-10-30 | 2019-01-08 | 河南科技大学 | A kind of production method of the stomach section silica gel model of sheep |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3428470A (en) * | 1966-06-09 | 1969-02-18 | Dow Chemical Co | Biological specimen in a cross-linked water-swellable acrylamide type resin and the incorporation of the specimen therein |
| US3638709A (en) * | 1969-12-11 | 1972-02-01 | Dow Chemical Co | Method of suspending immobilized biological specimens in a transparent gel in a transparent container |
| CN88105221A (en) * | 1988-03-08 | 1988-10-19 | 王向前 | Preparation method of color-preserving preservative for biological specimens |
| CN101718745A (en) * | 2009-12-07 | 2010-06-02 | 南京农业大学 | Dimensional electrophoresis method for total protein of jute root system |
-
2013
- 2013-11-14 CN CN201310567766.3A patent/CN103601844B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3428470A (en) * | 1966-06-09 | 1969-02-18 | Dow Chemical Co | Biological specimen in a cross-linked water-swellable acrylamide type resin and the incorporation of the specimen therein |
| US3638709A (en) * | 1969-12-11 | 1972-02-01 | Dow Chemical Co | Method of suspending immobilized biological specimens in a transparent gel in a transparent container |
| CN88105221A (en) * | 1988-03-08 | 1988-10-19 | 王向前 | Preparation method of color-preserving preservative for biological specimens |
| CN101718745A (en) * | 2009-12-07 | 2010-06-02 | 南京农业大学 | Dimensional electrophoresis method for total protein of jute root system |
Non-Patent Citations (3)
| Title |
|---|
| 刘仰斌等: ""改良人体标本保存液配方的效果"", 《中国组织工程研究与临床康复》 * |
| 李成文: "《现代免疫化学技术》", 30 April 1992, 上海科学技术出版社 * |
| 王廷华等: "《抗体理论技术》", 31 March 2005, 北京：科学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042622A (en) * | 2018-09-28 | 2018-12-21 | 河南科技大学 | A kind of guarantor's color fixing agent and preparation method thereof and the application in prepared by sample |
| CN109166439A (en) * | 2018-10-30 | 2019-01-08 | 河南科技大学 | A kind of production method of the stomach section silica gel model of sheep |
| CN109166439B (en) * | 2018-10-30 | 2020-11-13 | 河南科技大学 | Method for manufacturing sheep gastric section silica gel model |
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| CN103601844B (en) | 2016-02-10 |
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Inventor after: Li Chunbin Inventor after: Ma Hongfeng Inventor after: Zhang Zhongjun Inventor before: Ma Hongfeng Inventor before: Li Chunbin Inventor before: Zhang Zhongjun |
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Effective date of registration: 20160113 Address after: 116600 Dalian economic and Technological Development Zone, Liaohe Road, No. 18, Liaoning Applicant after: Dalian ethnic university Applicant after: Dalian Jinguiyuan Technology Development Co., Ltd. Address before: 116600 Harbin Road, Liaoning city of Dalian Province Economic and Technological Development Zone No. 34 Dalian Jingui edge technology development Co. Ltd. Applicant before: Dalian Jinguiyuan Technology Development Co., Ltd. |
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