CN104782684B - A kind of trees Wound protective agent and its preparation, application method - Google Patents
A kind of trees Wound protective agent and its preparation, application method Download PDFInfo
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Abstract
The present invention relates to ornamental trees and shrubs Conservative restoration field, and in particular to a kind of protective agent and its preparation, use for being used to damage trees wound reparation, counts, is made up of following components in parts by weight：9 ~ 11 parts of sulphur colloidal suspension agent, 9 ~ 11 parts of trimethylglycine, 0.4 ~ 0.6 part of benzac, 10 ~ 20 parts of alcohol, 0.15 ~ 0.25 part of high fat film emulsion, 0.1 ~ 0.2 part of bactericide, 900 ~ 1100 parts of pepper water.The present invention is to trees wound, rotten place, because of insect pest or caused by rotting at hole, bark damage cracking or the place that comes off can play the effect of long-acting disinfection sterilization and anticorrosion, accelerate Callus formation, the superinfection of germ insect pest is prevented, the band girdle mouth for promoting to cut saw kerf, do not heal heals rapidly, prevents disease pest from infecting.
Description
Technical field
The present invention relates to ornamental trees and shrubs Conservative restoration field, and in particular to a kind of guarantor for being used to damage trees wound reparation
Protect agent and its preparation, use.
Background technology
In trees daily management, wound, bark breakage easily make viral germ or insect pest invade people and disease occurs, rainwater
Elution make again wound occur it is wooden rot, physiological disturbance occurs for bad skin, gently then influences tree grafting growth and survives and grow, heavy then make
Into tree death, economic loss is brought to operator.Trimming (after saw) in the speed 1 year of the general edge skin of wound only
In a cm, the wound of branch small area, could heal within 1 year.Therefore, trees repairing, cut the wound after rear or trunk broken skin
Mouth protection processing, is a very important technical measures in cultivation and management of tree.
But at present, various wound healing agent for plants that in the market is sold, healing ointment etc., not only commodity price is high, uses
Wound healing and sterilizing disease-preventing are ineffective afterwards, and some, which does not have even, promotes wound healing and sterilization.
And plants wound protective agent on the market, consolidant, healing ointment etc. at present, when often declaring the maintenance after smearing
Between at 7 ~ 30 days or so, but typically at 7 days ~ 10 days or so, be unable to maintain that preferably protection, healing effect, bacterium for killing insect effect
Decline, wound is easily dry and cracked so that trees can not heal completely.
In addition, cause trunk to rot trees position that is more serious and producing hole for disease pest harm because insect pest eat into
Xylem causes to have on trunk that hole is deeper, although applying insecticide control kills insect, because worm channel bacteria infection causes
Bark and xylem partial decomposition, the infection area that rots is larger, while has hole again, easy to be dry and cracked, foregoing on the market each
Kind wound healing agent for plants, healing ointment can not play a preferable healing effect to such case
In summary, it is now known that the product such as trees Wound protective agent, for trees because of insect pest, rot, trimming wound
A more complete good protecting effect is unable to reach Deng processing.
If a kind of safe and efficient and practical trees Wound protective agent can be developed, it can be directed to because of insect pest, rot, trimming
All kinds of situations such as wound carry out preferably protection and promote healing, while can be directed to the larger rotten region of trees and/or more
Worm hole situation can equally play protection healing effect, energy acute activation plant immune system, promote cell division and growth metabolism,
Suppress germ hereby raw procreation, rehabilitation plant limb thickness wound, accelerating wound healing, asked with solving woods fruit tree wound healing
Topic, the cultivation of garden fruit tree wood will be very beneficial for.
The content of the invention
It is an object of the invention to provide a kind of trees Wound protective agent and its preparation, application method, to solve the above-mentioned back of the body
The problem of in scape technology.
To realize the object of the invention, the technical scheme that uses for：
A kind of trees Wound protective agent, it is characterised in that：Count, be made up of following components in parts by weight：Sulphur colloidal suspension agent 9
~ 11 parts, 9 ~ 11 parts of trimethylglycine, 0.4 ~ 0.6 part of benzac, 10 ~ 20 parts of alcohol, high fat film emulsion 0.15 ~ 0.25
Part, 0.1 ~ 0.2 part of bactericide, 900 ~ 1100 parts of pepper water；
The pepper water is counted in parts by weight, is made up of following components：95 ~ 105 parts of water, 4 ~ 6 parts of Chinese prickly ash.
Further, the bactericide includes one in octicin solution Fluoxastrobin, thiophanate methyl, Bravo, copper rosinate
Kind.
Further, a kind of preparation method of trees Wound protective agent is as follows：
A. prepared by pepper water：Count in parts by weight, water intaking, Chinese prickly ash, water is poured into pot, is put into Chinese prickly ash, boiled after boiled to
Go out pepper flavor, when pot reclaimed water is left 45% ~ 55%, filtering discards standby after residue cools down；
B. by bactericide, dissolved with Part I pepper water standby；
C. by trimethylglycine, dissolved with Part II pepper water standby；
D. by 50% sulphur colloidal suspension agent, dissolved with Part III pepper water standby；
E. it is standby after being dissolved with alcohol by benzac；
F. 27% high fat film emulsion is diluted with Part IV pepper water；
G. mix：Standby bactericide, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film are fallen successively
Enter container, add surplus pepper water constant volume, fully whisk mixing, solution is produced into viscoloid shape.
A kind of application method of trees Wound protective agent as described above, it is characterised in that：Trees wound is applied to, it is rotten
Rotten place, because of insect pest or caused by rotting at hole, bark damage cracking or the place that comes off.
Further, the trees wound that is applied to is specially the band girdle mouth for newly being cut to trees, sawing wound, not healing,
20 ~ 100ml Wound protective agents, even spread plants wound are taken with the hairbrush libation at an ancient wedding ceremony.
Further, the rotten place that is applied to is specially to xylophyta trunk rot, dry rot, ring spot knife
Deep standardized long circle, intersects at middle part and draws a number knife around along sick scar, as deep as health tissues or xylem, then take 50 with the hairbrush libation at an ancient wedding ceremony ~
300ml Wound protective agents smear sick scar, every 18 ~ 22 days once, after continuous 2 times check whether the sick scar of necrosis comes off, new tissue
Whether grow, according to circumstances smeared again.
Further, it is described to be applied to because of insect pest or be specially to cause trunk for disease pest harm at hole caused by rotting
Rot position that is more serious and producing hole, first hole portion wooden slag of rotting is wiped off, after being rinsed with clear water, then with the hairbrush libation at an ancient wedding ceremony takes 50
~ 500ml Wound protective agents smear whole sick portion and hole, carry out single treatment after 15 days again.
Further, it is described to be applied to bark damage cracking or the place that comes off is specially locally de- to bark cracking or bark
Fall, exposed the ancient tree of xylem, first cut away the bark of dry rot along exposed xylem edge with knife, then carve the exposed portion of wound
The xylem divided, take 50 ~ 500ml Wound protective agents to smear with the hairbrush libation at an ancient wedding ceremony, once smeared again after 28 ~ 30 days.
The principle of the present invention is as follows：
Sulphur in the sulphur colloidal suspension agent being related in the present invention, is a kind of ancient desinsection, bactericide, inexpensive, and from nothing
Resistance, it is lasting over over thousands of year.Sulphur colloidal suspension agent is a kind of novel protective agent in inorganic thiophanate fungicide, to a variety of germs and
The pest and disease damages such as worm's ovum, mite class have preferable prevention effect, and have concurrently has killing action to bacterium, worm.Its main function mechanism is logical
Cross interference germ mitosis during spindle formation, so as to influence germ cell division, reach suppression and kill germ.
Sulphur colloidal suspension agent fine size, suspensibility are high, caking property is strong, have good dispersion, adherence and anti-rain drop erosion.
High fat film：It is a kind of pure white, fine and smooth cloudy aqueous material, has one layer of very thin fat using rear plant surface
Fat sorrel, germ can not extend or seldom extend after invaded plantses body and cause harm, so as to reach diseases prevention purpose because there is film control.
High fat film has preferable prevention effect to most of fungal diseases, and its diseases prevention is physical action, and germ will not develop immunity to drugs, this
Body is nontoxic, harmless, nontoxic to people, plant, fish.
The preferred octicin solution of bactericide of the present invention：It is a kind of amino acids systemic fungicide, active ingredient takes for glycine
For derivative, there is efficient, low toxicity, noresidue, and have preferable permeability, have to the latent germ in intrusion bark
Certain eradicant action, a variety of fungies, bacterium and disease caused by virus can be prevented and treated.Sterilization mechanism is solidification germ protein,
Suppress mycelia and the spore growth of germ, destroy germ cell membrane, suppress germ breathing, be denatured germ enzyme system, become enzyme
Property and rise antibacterial and bactericidal action, reach kill germ purpose.
Trimethylglycine（Amino acid humectant）：It is a kind of natural structure material amphoteric ion type moisturizing ingredient, extremely
It is soluble in water, energy activated cell vigor, improve trees body surface moisture holding capacity, keep moistening to prevent drying.It is very heat-resisting, it is acidproof
With it is alkaline-resisting, and use range is wide, easy to operate and safety and stability.
Benzac：It is a kind of basic element of cell division, cell division can be promoted to break up, delays plant vivo protein to drop
Solution, break plant tip advantage, promote collateral generation, delaying senility, extend plant life, its activity is all more fast than 6- benzyl amino
Purine, cytokinin are high.Plant easily absorbs, and can not be transported again in plant rapidly, after handling plants wound, favorable incentive office
Portion's cell division, accelerate wound healing effect.
Pepper water：There is the effect such as sterilization, sterilization, as the solvent of wound healing protective agent, can strengthen and improve protective agent
Disinfection, disinsection efficiency.
Applicant has been surprisingly found that, sulphur colloidal suspension agent and high fat film emulsion, trimethylglycine in pepper water as solvent, one
Rise when using with prominent effect, there is splendid dissolubility in pepper water, and can effectively extend the effect of sulphur colloidal suspension agent
Timeliness, and in the presence of high fat film emulsion, bark damage cracking or the place that comes off can be in the guarantor of trimethylglycine for a long time
Under wet effect, it is further dry and cracked to effectively prevent bark.
In trees wound, rotten place, because of insect pest or caused by rotting at hole when, surrounding bark is more or less
There is certain damage cracking or have come off, can be while protecting wound, promoting to heal using the present invention, surrounding bark
Cracking will not further be developed, germ, insect pest etc. is avoided and caused damage from the entrance of other approach.
Wound first employs trimethylglycine（Amino acid humectant）Added as NMF composition in trees Wound protection
In agent, in bark cracking, come off, and at the wound with sizing depth, long-acting can maintain certain humidity, and in high fat
Under the fatty sorrel that membrane component is formed, cooperated with fatty sorrel, both extended the fatty sorrel duration, and do not interfered with fat again
The membrane permeability of fat sorrel, fatty sorrel are not easy in a short time（1 ~ 7 day）Forfeiture acts on, in mid-term（7 ~ 15 days）It is more slow
Gradually air-dry, while benzac and bactericide were not easy in 7 ~ 15 days because fatty sorrel under the moisture retention effect
The reasons such as dry and cracked, weathering, gradual forfeiture act on, and effectively extend the acting duration of Wound protective agent of the present invention.
The invention has the advantages that：
First, the present invention is to trees wound, rotten place, because of insect pest or caused by rotting at hole, bark damage cracking or
The place that comes off can play the effect of long-acting disinfection sterilization and anticorrosion, accelerate Callus formation, prevent the superinfection of germ insect pest, promote
The band girdle mouth for cut saw kerf, not healing can be used rapidly and closed, and prevent disease pest from contaminating.
2nd, diaphragm can be quickly formed in plant otch after the present invention smears, the membrane permeability with soil moisture retention, was both prevented
Sealing point, the loss of nutrient, and tree body of not burning.
3rd, the present invention effectively facilitates the power of regeneration of callus, promotes trees damage location quickly to heal.
4th, the present invention is viscoloid shape, is conveniently applied to any position of trees, and cost is relatively low, and preparation method is simple,
Suitable for promoting.
Embodiment
The present invention is described in further detail below by specific examples of the implementation.
Embodiment 1：
A kind of trees Wound protective agent：
A. prepared by pepper water：Count in parts by weight, take 95 parts of water, 4 portions of Chinese prickly ashes, water is poured into pot, is put into Chinese prickly ash, it is boiled
After boil to going out pepper flavor, pot reclaimed water it is remaining 45% when, filtering discards standby after residue cooling, and the above-mentioned pepper waters of 900g are made；
B. by 0.1g octicin solutions, dissolved with 100g pepper waters standby；
C. by 9g trimethylglycines, dissolved with 100g pepper waters standby；
D. by 9g 50% sulphur colloidal suspension agent, dissolved with 100g pepper waters standby；
E. it is standby after being dissolved with 10g alcohol by 0.4g benzac；
F. 0.15g 27% high fat film emulsion is diluted with 100g pepper waters；
G. mix：Standby octicin solution, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film are fallen successively
Enter container, add surplus 500g pepper water constant volumes, fully whisk mixing, solution is produced into viscoloid shape.
The cinnamomum camphora tree newly transplanted, 45 ~ 50 centimeters of rice footpath, in order to reduce leaf transpiration dehydration during transplanting, leave behind trees bone
Frame, remaining branches and leaves, which is all cut, to be amputated, and takes the present invention to carry out smearing protection to trees overground part sub-cut saw wound with the libation at an ancient wedding ceremony, each wound
Mouthful smear 20 ~ 50ml the present invention, until new branches and leaves germination and growth had no cut saw wound occur obfuscation go mouldy it is rotten.
Embodiment 2：
A kind of trees Wound protective agent：
A. prepared by pepper water：Count in parts by weight, take 105 parts of water, 6 portions of Chinese prickly ashes, water is poured into pot, is put into Chinese prickly ash, burn
Boiled after opening to going out pepper flavor, pot reclaimed water it is remaining 55% when, filtering discards standby after residue cooling, and the above-mentioned pepper waters of 1100g are made；
B. by 0.2g octicin solutions, dissolved with 100g pepper waters standby；
C. by 11g trimethylglycines, dissolved with 100g pepper waters standby；
D. by 11g 50% sulphur colloidal suspension agent, dissolved with 100g pepper waters standby；
E. it is standby after being dissolved with 20g alcohol by 0.6g benzac；
F. 0.25g 27% high fat film emulsion is diluted with 100g pepper waters；
G. mix：Standby octicin solution, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film are fallen successively
Enter container, add surplus 700g pepper water constant volumes, fully whisk mixing, solution is produced into viscoloid shape.
For the rot of perennation trees, with knife along deep standardized circle around sick scar, intersect at middle part and draw a number knives, as deep as
Health tissues or xylem, take 50 ~ 100ml of protective agent of the present invention to be applied to disease scar position with the hairbrush libation at an ancient wedding ceremony, one was smeared every 20 days
It is secondary, the sick scar of necrosis is checked after continuous 2 times, it is most of to have come off, new tissue is grown, bad local area carries out one again to healing
Secondary brushing, it is almost complete to be cured.
Embodiment 3：
A kind of trees Wound protective agent：
A. prepared by pepper water：Count in parts by weight, take 100 parts of water, 5 portions of Chinese prickly ashes, water is poured into pot, is put into Chinese prickly ash, burn
Boiled after opening to going out pepper flavor, pot reclaimed water it is remaining 50% when, filtering discards standby after residue cooling, and the above-mentioned pepper waters of 1000g are made；
B. by 0.15g Fluoxastrobins, dissolved with 100g pepper waters standby；
C. by 10g trimethylglycines, dissolved with 100g pepper waters standby；
D. by 10g 50% sulphur colloidal suspension agent, dissolved with 100g pepper waters standby；
E. it is standby after being dissolved with 15g alcohol by 0.5g benzac；
F. 0.2g 27% high fat film emulsion is diluted with 100g pepper waters；
G. mix：Standby Fluoxastrobin, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film are fallen successively
Enter container, add surplus 600g pepper water constant volumes, fully whisk mixing, solution is produced into viscoloid shape.
Ammonium alum method Shu Age 200 years, 1.5 meters of trees rice footpath, on 50 centimeters away from ground trunks, because old carpenter worm is eaten into wooden
Portion causes have hole on trunk, applies insecticide control and kills insect, but because worm channel bacteria infection causes bark and xylem
Partial decomposition is wide 50 centimeters, as deep as 15 ~ 25 centimeters.After checking position of rotting, to rotting, position is handled, and is first removed and is wiped corruption off
Rotten wooden slag after clear water is rinsed well, takes 100 ~ 300ml of protective agent of the present invention carefully to smear until health tissues with the hairbrush libation at an ancient wedding ceremony, and 15
Checked after it, rotten symptom is controlled, then has been carried out continuously 2 ~ 3 processing, and every minor tick 20 days, empty position is healed substantially
Afterwards, with hole is filled up after yellow mud bar and pulverized limestone blending, improve and view and admire and commodity value by trunk as former state.
Embodiment 4：
A kind of trees Wound protective agent：
A. prepared by pepper water：Count in parts by weight, take 100 parts of water, 5 portions of Chinese prickly ashes, water is poured into pot, is put into Chinese prickly ash, burn
Boiled after opening to going out pepper flavor, pot reclaimed water it is remaining 50% when, filtering discards standby after residue cooling, and the above-mentioned pepper waters of 1000g are made；
B. by 0.15g Bravos, dissolved with 100g pepper waters standby；
C. by 10g trimethylglycines, dissolved with 100g pepper waters standby；
D. by 10g 50% sulphur colloidal suspension agent, dissolved with 100g pepper waters standby；
E. it is standby after being dissolved with 15g alcohol by 0.5g benzac；
F. 0.2g 27% high fat film emulsion is diluted with 100g pepper waters；
G. mix：Standby Bravo, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film are fallen successively
Enter container, add surplus 600g pepper water constant volumes, fully whisk mixing, solution is produced into viscoloid shape.
Have 12 plants of , Shu Age of ginkgo in 70 ~ 85 years in certain university campus, 70 ~ 80 centimeters of trunk rice footpath, due to it is artificial because
Element（Teachers and students, which take exercise, all the year round pats trees, nails, pulling cord airing clothes etc.）, cause 1.2 ~ 1.5 meters away from ground eminences of trunk
Bark local shedding, Naked expose xylem, and it is attractive in appearance both to have influenceed trunk, more influence arboreal growth.For this symptom, instruct campus green
Chemical industry people, the bark for the withered dry rot that sustained damage is cut away along exposed xylem edge with knife, then firmly carve and hinder exposed portion
Divide as deep as health tissues or xylem, take 200 ~ 300ml of protective agent of the present invention to be applied to damaged part with the hairbrush libation at an ancient wedding ceremony, apply again within 30 days
Smear once, twice in succession after, the bark that xylem edge is revealed along Naked is formed callus, and gradually inwardly extended.Hang up
Warning sign, the education for carrying out love trees in the students and faculty is incorporated in, rare trees is effectively protected.
It is present invention contrast common trees Wound protective agent effect table on the market below：
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (7)
- A kind of 1. trees Wound protective agent, it is characterised in that：Count, be made up of following components in parts by weight：Sulphur colloidal suspension agent 9 ~ 11 parts, 9 ~ 11 parts of trimethylglycine, 0.4 ~ 0.6 part of benzac, 10 ~ 20 parts of alcohol, high fat film emulsion 0.15 ~ 0.25 Part, 0.1 ~ 0.2 part of bactericide, 900 ~ 1100 parts of pepper water；The pepper water is counted in parts by weight, is made up of following components：Water 95 ~ 105 parts, 4 ~ 6 parts of Chinese prickly ash；The bactericide is included in octicin solution, Fluoxastrobin, thiophanate methyl, Bravo, copper rosinate It is a kind of.
- A kind of 2. trees Wound protective agent according to claim 1, it is characterised in that：A kind of trees Wound protective agent Preparation method it is as follows：A. prepared by pepper water：Count in parts by weight, water intaking, Chinese prickly ash, water is poured into pot, is put into Chinese prickly ash, boiled after boiled to going out flower Green pepper taste, when pot reclaimed water is left 45% ~ 55%, filtering discards standby after residue cools down；B. by bactericide, dissolved with Part I pepper water standby；C. by trimethylglycine, dissolved with Part II pepper water standby；D. by 50% sulphur colloidal suspension agent, dissolved with Part III pepper water standby；E. it is standby after being dissolved with alcohol by benzac；F. 27% high fat film emulsion is diluted with Part IV pepper water；G. mix：Standby bactericide, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film are poured into appearance successively Device, add surplus pepper water constant volume, fully whisk mixing, solution is produced into viscoloid shape.
- A kind of 3. application method of trees Wound protective agent as described in claim 1,2 any one, it is characterised in that：It is applied to Trees wound, rotten place, because of insect pest or caused by rotting at hole, bark damage cracking or the place that comes off.
- A kind of 4. application method of trees Wound protective agent according to claim 3, it is characterised in that：It is described to be applied to tree Wooden wound is specially that trees are newly cut, saw wound, the band girdle mouth not healed, and 20 ~ 100ml Wound protective agents are taken with the hairbrush libation at an ancient wedding ceremony, Even spread plants wound.
- A kind of 5. application method of trees Wound protective agent according to claim 3, it is characterised in that：It is described to be applied to corruption Rotten place is specially that xylophyta trunk rot, dry rot, ring spot knife are handed over along deep standardized long circle around sick scar at middle part Fork draws a number knives, takes 50 ~ 300ml Wound protective agents to smear sick scar as deep as health tissues or xylem, then with the hairbrush libation at an ancient wedding ceremony, every 18 ~ 22 days once, checks whether the sick scar of necrosis comes off after continuous 2 times, and whether new tissue grows, and is according to circumstances smeared again.
- A kind of 6. application method of trees Wound protective agent according to claim 3, it is characterised in that：It is described be applied to because Insect pest is specially to cause trunk to rot position that is more serious and producing hole for disease pest harm at hole caused by rotting, first Hole portion wooden slag of rotting is wiped off, after being rinsed with clear water, then with the hairbrush libation at an ancient wedding ceremony takes 50 ~ 500ml Wound protective agents to smear whole sick portion And hole, single treatment is carried out after 15 days again.
- A kind of 7. application method of trees Wound protective agent according to claim 3, it is characterised in that：It is described to be applied to tree Skin damage cracking or the place that comes off are specially to bark cracking or bark local shedding, have exposed the ancient tree of xylem, first with knife edge Exposed xylem edge to cut away the bark of dry rot, then carve the xylem for hindering exposed part, 50 ~ 500ml is taken with the hairbrush libation at an ancient wedding ceremony Wound protective agent is smeared, and is once smeared again after 28 ~ 30 days.
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| CN106258443A (en) * | 2016-09-06 | 2017-01-04 | 合肥元政农林生态科技有限公司 | A kind of high-quality mountain cultural method |
| CN106665240A (en) * | 2016-11-30 | 2017-05-17 | 蚌埠市众星蔬果科技专业合作社联合社 | Method for restoring rotten part of ancient metasequoia |
| CN107771870A (en) * | 2017-11-23 | 2018-03-09 | 广西垚宇贸易有限公司 | A kind of cinnamon bark protective agent and preparation method |
| CN107950602A (en) * | 2017-12-14 | 2018-04-24 | 柳州市象行教育科技有限责任公司 | A kind of special consolidant of callus and preparation method thereof |
| CN109041971A (en) * | 2018-07-27 | 2018-12-21 | 南京林业大学 | A kind of trees wound dermatoplastic method |
| CN109258177B (en) * | 2018-11-12 | 2021-03-30 | 王君 | Method for repairing wound of lacquer tree |
| CN111955490A (en) * | 2020-09-01 | 2020-11-20 | 开封市劳恩格润农业科技有限公司 | Fruit tree wound healing agent and application method thereof |
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| CN103621338A (en) * | 2013-10-27 | 2014-03-12 | 大连东芳果菜专业合作社 | New method for treating wounds of fruit trees |
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| CN101120678A (en) * | 2007-09-07 | 2008-02-13 | 山东省果树研究所 | Gummosis protective therapeutic agent and its preparation method |
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| CN102617224A (en) * | 2012-03-07 | 2012-08-01 | 海南正业中农高科股份有限公司 | Plant wound protective agent containing chitosan oligosaccharide |
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