CN107950602A - A kind of special consolidant of callus and preparation method thereof - Google Patents
A kind of special consolidant of callus and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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Abstract
The present invention relates to consolidant processing technique field, more particularly to a kind of special consolidant of callus and preparation method thereof, the consolidant of the application is made of potato composite essential oil, pepper extract, pepper extract, Folium Artemisiae Argyi extract, borax and sodium chloride solution；In consolidant, potato composite essential oil includes potato derived essential oil and auxiliary material derived essential oil, by potato essential oil and auxiliary material essential oil compounding use, can improve the DPPH free radical scavenging activities of potato derived essential oil, improve the inoxidizability of essential oil；Contain abundant capsaicine and capsaicine in the pepper extract of consolidant；Contain the unrighted acids such as abundant Zanthoxylum essential oil, alpha linolenic acid in pepper extract；There are anti-inflammatory, sterilizing and promote cell differentiation, the effect of metabolism；Sterilization is played to the vegetable material of vegetative propagation containing abundant Flavonoid substances, folium eucalypti alkanes material and triterpene substance in Folium Artemisiae Argyi extract, promotees differentiation, improves the effect of germination percentage.
Description
【Technical field】
The present invention relates to consolidant processing technique field, the special consolidant of more particularly to a kind of callus and its preparation side
Method.
【Background technology】
The vegetative propagation of plant can make the plant of breeding keep its maternal all excellent hereditary shape, therefore, vegetative propagation
Method is widely used in the breeding of high quality tree species, and at present, the common method of vegetative propagation mainly has cuttage, grafting and tissue training
Support, but either cuttage, grafting or tissue cultures are required for intercepting propagating materials from female parent, and have in propagating materials scarce
Mouthful, wound infection is be easy to cause, the survival rate of propagating materials can be influenced, it is at present, more during these propagating materials are handled
It is to handle propagating materials using steroids root-inducing promoter, although being improved to some extent to the survival rate of propagating materials,
But still it is undesirable, this is because steroids root-growing agent does not have the effect of sterilizing, it is impossible to suppress the growth of pathogen, and
And multi-purpose disinfectant is Hg disinfectants, Hg in plant can be caused to remain, nor be adapted to the growth of certain plants, for this reason,
It is necessary to find out a kind of consolidant of suitable plant callus growth, the chemical residues such as Hg will not be caused, can promote to plant
Thing wound healing, cell differentiation, improves the oxidation resistance of wound, prevents plant notch from being aoxidized and causing the low skill of germination percentage
Art defect.
【The content of the invention】
In view of the above, it is necessary to find out a kind of consolidant of suitable plant callus growth, Hg etc. will not be caused
Chemical residue, can promote plant wound heeling, cell differentiation, improves the oxidation resistance of wound, prevent plant notch quilt
Aoxidize and cause the low technological deficiency of germination percentage.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
A kind of special consolidant of callus, the special consolidant comprise the following components in parts by weight：11 parts -13 parts
Potato composite essential oil, 12 parts -18 parts of pepper extract, 15 parts -25 parts of pepper extract, 20 parts -25 parts of folium artemisiae argyi
Extract, 5 parts -7 parts of borax and 110 parts -120 parts of mass concentration are 2% sodium chloride solution.
Further, potato essential oil and weight of the potato composite essential oil by percentage by weight for 70%-90%
The auxiliary material essential oil that percentage is 10%-30% forms.
Further, the potato essential oil is made by potato；The auxiliary material essential oil by following parts by weight component system
：17 parts -29 parts of Chinese toon wood skin, 13 parts -31 parts of Radix agastaches, 24 parts -32 parts of Semen sesami nigrum and 34 parts -46 parts of the leaf of bamboo.
Further, the potato composite essential oil preparation method include potato extraction of essential oil, auxiliary material extraction of essential oil and
Essential oil mixes three steps and is made, and comprises the following steps that：
(1) potato extraction of essential oil：
Step S1：Potato is puffing：Potato is cut into the potato block that thickness is 1-3mm, then potato block is put into
Temperature is to soak 1-2h in 0-2 DEG C of frozen water, potato block is taken out drain be totally submerged keep 1-2s in rapeseed oil after take
Go out, be put into steamer cook, mashed into paste, it is 9MPa-10MPa that mashed potato then is put into pressure condition, temperature 100
Keep 10min-15min in DEG C -105 DEG C of hot wind, pressure release, then it is 0MPa-2MPa that mashed potato is put into pressure condition, temperature
It is puffing to keep 5min-10min to complete potato in 0 DEG C -5 DEG C of cold wind；
Step S2：Cellulase treatment：By potatos of the step S1 after puffing and biology enzyme I solution according to solid-liquid mass ratio
For 1:1-2 mixing immersions, 20min-30min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes to remove water
Get potato flour；Wherein, biology enzyme I solution is made of the raw material of following parts by weight：30 parts -35 parts of amylase, 20
Parts -25 parts carbohydrase, 5 parts -10 parts of aspergillus oryzae, 10 parts -20 parts of barbaloin and 250 parts of -300 parts of mass percents are
1% sodium hydroxide solution；The enzyme activity of the amylase is 800u/g-1000u/g；The enzyme activity of the carbohydrase is
1000u/g-1200u/g；The living bacteria count of the aspergillus oryzae is 3.1 × 107~6.7 × 107CFU/g；
Step S3：Microwave treatment：After the potato flour of step S2 is cooled to room temperature, it is laid in plate, tiling is thick
Spend for 1mm-3mm, be placed on microwave oven turntable and carry out microwave treatment, the frequency of the microwave treatment is 2300MHZ, and power is
1000W, total duration 180s；
Step S4：Supercritical CO2Extraction：By in the potato flour input extraction kettle after step S3 microwave treatments, pressing
When power is 15-20MPa, with liquid CO2Static extracting 30-40min, then progressively discharges pressure, carries out supercritical CO2Dynamic extracts
Take, dynamic extraction technological parameter is：When temperature is heated to 40-50 DEG C, pressurize to system, pressure reaches 40-50MPa
Afterwards, CO is adjusted2Flow is 15-25L/min, keeps constant temperature and pressure to discharge after carrying out cycling extraction 2-4h, in 800-1000r/min
Under conditions of be centrifuged to obtain potato essential oil.
(2) auxiliary material extraction of essential oil：
Step A1：Auxiliary material is puffing：Each raw material is weighed by above-mentioned parts by weight to be crushed respectively, by -50 mesh sieve net of 30 mesh
Screening, is then 1 according to solid-liquid mass ratio by ethanol solution that Chinese toon wood skin powder and percentage by volume are 75%:2 mixing；By the leaves of pulse plants
The butanol solution that spiceleaf powder is 10% with percentage by volume is 1 according to solid-liquid mass ratio:1 mixing；By Semen sesami nigrum powder and body
The ethanol solution that product percentage is 75% is 1 according to solid-liquid mass ratio:1 mixing；By leaf of bamboo powder and water according to solid-liquid mass ratio
For 1:1 mixing；Then each powder soak is placed in the refrigerator-freezer that temperature is -2 DEG C~0 DEG C and refrigerates 24h-26h；Filtering, takes each
Powder mixes, and it is 8MPa-9MPa to be put into after being infiltrated in rapeseed oil mixed-powder being put into pressure condition, and temperature is 110 DEG C -120
DEG C hot wind in keep 20min-25min, pressure release, then it is 0MPa-2MPa that mashed potato is put into pressure condition, temperature for 0 DEG C-
15min-20min is kept to complete auxiliary material in 5 DEG C of cold wind puffing；
Step A2：Cellulase treatment：By auxiliary materials of the step S1 after puffing and biology enzyme II solution according to solid-liquid mass ratio
For 1:1-2 mixing immersions, 20min-30min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes to remove water
Get auxiliary material dry powder；Wherein, biology enzyme II solution is made of the raw material of following parts by weight：15 parts -25 parts of pectase, 30
Cellulase, 20 parts -30 parts of amylase, 10 parts -20 parts of aspergillus flavus and 300 parts of -350 parts of mass percents of -50 parts of part
For 2% sodium chloride solution；The enzyme activity of the pectase is 1200u/g-1500u/g；The enzyme activity of the cellulase is
1500u/g-2000u/g；The enzyme activity of the amylase is 900u/g-1200u/g；The living bacteria count of the aspergillus flavus is
3.9×108~6.7 × 108CFU/g；
Step A3：Supercritical CO2Extraction：The auxiliary material dry powder of step S2 is put into extraction kettle, is 15-20MPa in pressure
When, with liquid CO2Static extracting 20-30min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic extraction work
Skill parameter is：When temperature is heated to 50-60 DEG C, pressurize to system, after pressure reaches 30-40MPa, adjust CO2Flow
For 15-25L/min, keep constant temperature and pressure to discharge after carrying out cycling extraction 3-5h, carried out under conditions of 800-1000r/min from
The isolated auxiliary material essential oil of the heart.
(3) essential oil mixes：
Potato essential oil and auxiliary material essential oil are weighed according to percentage by weight, is 75% by potato essential oil and percentage by volume
Ethanol solution according to 2:1 is mixed, and 5min-7min is stirred under the speed conditions of 500-800r/min, is then added
Auxiliary material essential oil, continues to stir 6min-8min, rotary evaporation concentration goes moisture removal that potato composite precision described herein is made
Oil.
Present invention also provides a kind of preparation method for preparing the special consolidant of callus, this method includes following step
Suddenly：
(1) each material composition is weighed by weight；
(2) after potato composite essential oil is mixed with the sodium chloride that mass concentration is 2%, it is put into homogenizer and stirs
Emulsion is made, and the pH value of emulsion is adjusted to 6.8-7.5, is placed in the refrigerator-freezer that temperature is -2 DEG C~0 DEG C and refrigerates
30min-50min obtains potato composite essential oil emulsion, and the mixing time is 20min-30min；Speed of agitator is 400r/
min-600r/min；
(3) borax is added into the potato composite essential oil emulsion of step (2), and it is right that pH value is adjusted to 5.2-5.9
After add after pepper extract, pepper extract and Folium Artemisiae Argyi extract be sufficiently stirred, be put into Ultrasound Instrument and be ultrasonically treated,
The time of supersound process is 40min-50min, and it is 40 DEG C~50 DEG C to be ultrasonically treated temperature, obtains the special healing of the callus
Agent.
The present invention has the advantages that：
1st, the special consolidant of the callus of the application is by potato composite essential oil, pepper extract, pepper extract, Chinese mugwort
The sodium chloride solution that leaf extract, borax and mass concentration are 2% forms, and in consolidant, potato composite essential oil is included from horse
The derived essential oil extracted in bell potato and the auxiliary material derived essential oil extracted from auxiliary material, are compounded by potato essential oil and auxiliary material essential oil
Use, the DPPH free radical scavenging activities of potato derived essential oil can be improved, improve the inoxidizability of essential oil.Carried in potato essential oil
The essential oil taken contains abundant lactone component, can effectively improve the essential oil extracted in essential oil fragrance Chinese toon wood skin and relatively be enriched with oxygen-containing chemical combination
Thing, can improve the oxidation resistance of essential oil；The essential oil extracted in Radix agastaches can improve the sterilization of essential oil compared with beneficiation of hydrocarbons compound
Ability, so as to improve oxidation resistance；The essential oil extracted in Semen sesami nigrum is relatively enriched with oxygenatedchemicals component, so as to improve anti-oxidant
Ability；The essential oil extracted in the leaf of bamboo is relatively enriched with oxygenatedchemicals component, so as to improve oxidation resistance.When in auxiliary material derived essential oil
After being used in conjunction with each other containing Chinese toon wood skin, Semen sesami nigrum and several components of the leaf of bamboo, the oxygenatedchemicals component of essential oil can be significantly improved
Improve the oxidation resistance of essential oil；There are anti-inflammatory, sterilizing containing abundant capsaicine and capsaicine in pepper extract and promote thin
Born of the same parents' differentiation, the effect of metabolism；Contain the unrighted acids such as abundant Zanthoxylum essential oil, alpha-linolenic acid in pepper extract, its
Middle Zanthoxylum essential oil can play the effect of bactericidal antiphlogistic, and unrighted acid can provide one layer of oily protective film for wound, can effectively hinder
The only superinfection problem of harmful bacteria；It can promote the cell differentiation of plant in Folium Artemisiae Argyi extract containing abundant Flavonoid substances,
Promote plant establishment, folium eucalypti alkanes material and triterpene substance can kill the pathogenic bacteria such as anthracnose, have broad-spectrum sterilization effect.
2nd, the application potato first carries out puffing, microwave treatment and cellulase treatment during essential oil is extracted, due to
Its essential oil of the potato of the application and Vitamin C content ratio starch and protein content component are low, and starch, protein material
With viscosity, the small-molecule substance such as essential oil and vitamin C cannot be separated out effectively, if, it is impossible to its potato is pre-processed
The output capacity of essential oil will be largely effected on, is soaked in the present frozen water of potato, can remove most starch material, Ma Ling
Potato chips will cause most starch chain and protein hydrogen bonds to be broken after high pressure, low pressure are puffing, with biology after crushing
Enzyme and microwave are handled, and can effectively remove content of starch, destroy the molecular structure of starch and protein, and this temperature, pressure
Power will not destroy derived essential oil again, will can effectively facilitate the precipitation efficiency of essential oil, improve the essential oil yield of potato；Meanwhile this
Apply for the supercritical CO used2Extraction prepares potato essential oil, and this method is easy to operate, ripe, and preparation rate is high, purity
It is high miscellaneous few；The characteristics of the application is directed to starch in potato, protein content is more is configured with biology enzyme I solution, forms sediment in enzyme solutions
Powder enzyme can effectively starch-splitting, carbohydrase starch can be decomposed into the glucose of small molecule, aspergillus oryzae containing abundant viable bacteria into
Point, can effective decomposing protein, barbaloin can effectively improve aspergillus oryzae activity, sodium hydroxide solution can be biology enzyme I solution carry
For enzymolysis environment preferably；The characteristics of it is mostly plant component that the application, which is directed in auxiliary material, and content of cellulose is high is configured with biology
Enzyme II solution, pectase can effectively decompose the pectin composition in plant in enzyme solutions, and cellulase can be decomposed effectively in plant
Cellulose components, amylase can effectively decompose starch enzyme component in plant, in aspergillus flavus containing abundant viable bacteria component, can be effective
Promote the decomposition of cellulose, sodium chloride solution can provide enzymolysis environment preferably for biology enzyme II solution.
【Embodiment】
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1：
The special consolidant of the present embodiment callus comprises the following components in parts by weight：11 parts of potato composite precision
Oil, 12 parts of pepper extract, 15 parts of pepper extract, 20 parts of Folium Artemisiae Argyi extract, 5 parts of borax and 110 parts of quality
Concentration is 2% sodium chloride solution.
The extracting method of pepper extract is in the present embodiment：By moisture content to be smashed to pieces after 5% chilli fruit remove seed,
Then it is 1 according to solid-liquid mass ratio with the acetone soln of 1000mg/g:4 are mixed, 60 DEG C water-bath medium temperatures soak 4 times, often
Secondary 1h, filtering, merging filtrate, is recovered under reduced pressure acetone, and to paste, the relative density of thick paste thing is 2.76g/cm3Obtain described
Pepper extract, the content of capsaicine is 143.76mg/g in extract, and the content of capsaicine is 95.65mg/g.
The extracting method of pepper extract is in the present embodiment：Smash the dried pepper that moisture content is 5% to pieces rear and volume hundred
The ethanol solution that fraction is 75% is according to solid-liquid mass ratio 1:2 are mixed, and are carried out in the refluxing extraction device that temperature is 115 DEG C
Refluxing extraction, refluxing extraction 5h, filters to take filtrate and is evaporated under reduced pressure to paste, the relative density of paste is 2.54g/cm3
Obtain the pepper extract, the content of Zanthoxylum essential oil is 128.07mg/g in extract, and alpha-linolenic acid content is 83.09mg/g.
The extracting method of Folium Artemisiae Argyi extract is in the present embodiment：By the dry moxa leaf and the oil of 480mg/g that moisture content is 5%
Ether is 1 according to mass ratio:3 are mixed, and are extracted 4h in the water bath with thermostatic control that temperature is 55 DEG C, are filtered to take filtrate afterwards and subtracted
Pressing distillation and concentration, the relative density of paste is 1.98g/cm to paste3Obtain the Folium Artemisiae Argyi extract, flavonoids in extract
Content of material is 90.83mg/g, and folium eucalypti alkanes content of material is 67.92mg/g, triterpenes content 123.09mg/g.
Wherein, the potato essential oil and percentage by weight that potato composite essential oil is 70% by percentage by weight are 30%
Auxiliary material essential oil forms, and the potato essential oil of the present embodiment is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：
17 parts of Chinese toon wood skin, 13 parts of Radix agastaches, 24 parts of Semen sesami nigrum and 34 parts of the leaf of bamboo.
In the present embodiment, potato composite essential oil preparation method includes potato extraction of essential oil, auxiliary material extraction of essential oil and essence
Three steps of oil mixing are made, and comprise the following steps that：
(1) potato extraction of essential oil：
Step S1：Potato is puffing：Potato is cut into the potato block that thickness is 1mm, then potato block is put into temperature
Spend in the frozen water for 0 DEG C and soak 1h, potato block is taken out to drain to be totally submerged in rapeseed oil and is taken out after holding 1s, is put into steaming
Cooked in pot, mashed into paste, it is 9MPa that mashed potato then is put into pressure condition, and temperature is to be kept in 100 DEG C of hot wind
10min, pressure release, then it is 0MPa that mashed potato is put into pressure condition, keeps 5min to complete potato in the cold wind that temperature is 0 DEG C
It is puffing；
Step S2：Cellulase treatment：By potatos of the step S1 after puffing and biology enzyme I solution according to solid-liquid mass ratio
For 1:1 mixing immersion, 20min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes moisture removal to obtain horse
Bell potato dry powder；Wherein, biology enzyme I solution is made of the raw material of following parts by weight：30 parts of amylase, 20 parts of carbohydrase, 5 parts
Aspergillus oryzae, 10 parts of barbaloin and 250 parts of mass percents be 1% sodium hydroxide solution；The enzyme activity of the amylase
For 800u/g；The enzyme activity of the carbohydrase is 1000u/g；The living bacteria count of the aspergillus oryzae is 3.1 × 107；
Step S3：Microwave treatment：After the potato flour of step S2 is cooled to room temperature, it is laid in plate, tiling is thick
Spend for 1mm, be placed on microwave oven turntable and carry out microwave treatment, the frequency of the microwave treatment is 2300MHZ, and power is
1000W, total duration 180s；
Step S4：Supercritical CO2Extraction：By in the potato flour input extraction kettle after step S3 microwave treatments, pressing
When power is 15-20MPa, with liquid CO2Static extracting 30min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction,
Dynamic extraction technological parameter is：When temperature is heated to 40 DEG C, pressurize to system, after pressure reaches 40MPa, adjust CO2
Flow is 15L/min, keeps constant temperature and pressure to discharge after carrying out cycling extraction 2h, is centrifuged under conditions of 800r/min
Obtain potato essential oil.
(2) auxiliary material extraction of essential oil：
Step A1：Auxiliary material is puffing：Each raw material is weighed by above-mentioned parts by weight to be crushed respectively, is selected by 30 mesh sieve mesh screens,
Then it is 1 according to solid-liquid mass ratio by ethanol solution that Chinese toon wood skin powder and percentage by volume are 75%:2 mixing；By Radix agastaches
The butanol solution that powder is 10% with percentage by volume is 1 according to solid-liquid mass ratio:1 mixing；By Semen sesami nigrum powder and volume hundred
The ethanol solution that fraction is 75% is 1 according to solid-liquid mass ratio:1 mixing；According to solid-liquid mass ratio it is 1 by leaf of bamboo powder and water:1
Mixing；Then each powder soak is placed in the refrigerator-freezer that temperature is -2 DEG C and refrigerates 24h；Filtering, takes each powder to mix, is put into dish
It is 8MPa that mixed-powder is put into pressure condition after being infiltrated in seed oil, and temperature is that 20min is kept in 110 DEG C of hot wind, pressure release, then
It is 0MPa that mashed potato is put into pressure condition, keeps 15min to complete auxiliary material in the cold wind that temperature is 0 DEG C puffing；
Step A2：Cellulase treatment：By auxiliary materials of the step S1 after puffing and biology enzyme II solution according to solid-liquid mass ratio
For 1:1 mixing immersion, 20min is stood in temperature is 27 DEG C of insulating box, is then concentrated, dry to go moisture removal to obtain auxiliary
Expect dry powder；Wherein, biology enzyme II solution is made of the raw material of following parts by weight：15 parts of pectase, 30 parts of cellulase, 20
The sodium chloride solution that the amylase, 10 parts of aspergillus flavus and 300 parts of mass percents of part are 2%；The enzyme activity of the pectase
For 1200u/g；The enzyme activity of the cellulase is 1500u/g；The enzyme activity of the amylase is 900u/g；The aspergillus flavus
Living bacteria count be 3.9 × 108；
Step A3：Supercritical CO2Extraction：The auxiliary material dry powder of step S2 is put into extraction kettle, when pressure is 15MPa,
With liquid CO2Static extracting 20min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic extraction technological parameter
For：When temperature is heated to 50 DEG C, pressurize to system, after pressure reaches 30MPa, adjust CO2Flow is 15L/min, is protected
Hold after constant temperature and pressure carries out cycling extraction 3h and discharge, be centrifuged to obtain auxiliary material essential oil under conditions of 800r/min.
(3) essential oil mixes：
Potato essential oil and auxiliary material essential oil are weighed according to percentage by weight, is 75% by potato essential oil and percentage by volume
Ethanol solution according to 2:1 is mixed, and 5min is stirred under the speed conditions of 500r/min, then adds auxiliary material essential oil,
Continue to stir 6min, rotary evaporation concentration goes moisture removal to be made.
The preparation method of the special consolidant of the present embodiment callus includes the following steps：
(1) each material composition is weighed by weight；
(2) after potato composite essential oil is mixed with the sodium chloride that mass concentration is 2%, it is put into homogenizer and stirs
Emulsion is made, and the pH value of emulsion is adjusted to 6.8, is placed on refrigeration 30min in the refrigerator-freezer that temperature is -2 DEG C and obtains horse
Bell potato composite essential oil emulsion, the mixing time are 20min；Speed of agitator is 400r/min；
(3) borax is added into the potato composite essential oil emulsion of step (2), and then pH value is adjusted to 5.2 again
After addition pepper extract, pepper extract and Folium Artemisiae Argyi extract are sufficiently stirred, it is put into Ultrasound Instrument and is ultrasonically treated, ultrasound
The time of processing is 40min, and it is 40 DEG C to be ultrasonically treated temperature, obtains the special consolidant of the callus.
Embodiment 2：
The special consolidant of the present embodiment callus comprises the following components in parts by weight：13 parts of potato composite precision
Oil, 18 parts of pepper extract, 15 parts -25 parts of pepper extract, 25 parts of Folium Artemisiae Argyi extract, 7 parts of borax and 120 parts
Mass concentration is 2% sodium chloride solution.
The extraction of the extracting method of pepper extract, the extracting method of pepper extract, Folium Artemisiae Argyi extract in the present embodiment
Method and embodiment 1 are completely the same.
Wherein, the potato essential oil and percentage by weight that potato composite essential oil is 90% by percentage by weight are 10%
Auxiliary material essential oil forms, and the potato essential oil of the present embodiment is made by potato；The auxiliary material essential oil of the present embodiment is by following parts by weight
Component be made：29 parts of Chinese toon wood skin, 31 parts of Radix agastaches, 32 parts of Semen sesami nigrum and 46 parts of the leaf of bamboo.
In the present embodiment, potato composite essential oil preparation method includes potato extraction of essential oil, auxiliary material extraction of essential oil and essence
Three steps of oil mixing are made, and comprise the following steps that：
(1) potato extraction of essential oil：
Step S1：Potato is puffing：Potato is cut into the potato block that thickness is 3mm, then potato block is put into temperature
Spend in the frozen water for 2 DEG C and soak 2h, potato block is taken out to drain to be totally submerged in rapeseed oil and is taken out after holding 2s, is put into steaming
Cooked in pot, mashed into paste, it is 10MPa that mashed potato then is put into pressure condition, and temperature is to be kept in 105 DEG C of hot wind
15min, pressure release, then it is 2MPa that mashed potato is put into pressure condition, keeps 5min-10min to complete in the cold wind that temperature is 5 DEG C
Potato is puffing；
Step S2：Cellulase treatment：By potatos of the step S1 after puffing and biology enzyme I solution according to solid-liquid mass ratio
For 1:2 mixing immersions, 30min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes moisture removal to obtain horse
Bell potato dry powder；Wherein, biology enzyme I solution is made of the raw material of following parts by weight：35 parts of amylase, 25 parts of carbohydrase, 10
The sodium hydroxide solution that the aspergillus oryzae, 20 parts of barbaloin and 300 parts of mass percents of part are 1%；The enzyme activity of the amylase
Power is 1000u/g；The enzyme activity of the carbohydrase is 1200u/g；The living bacteria count of the aspergillus oryzae is 6.7 × 107CFU/
g；
Step S3：Microwave treatment：After the potato flour of step S2 is cooled to room temperature, it is laid in plate, tiling is thick
Spend for 3mm, be placed on microwave oven turntable and carry out microwave treatment, the frequency of the microwave treatment is 2300MHZ, and power is
1000W, total duration 180s；
Step S4：Supercritical CO2Extraction：By in the potato flour input extraction kettle after step S3 microwave treatments, pressing
When power is 20MPa, with liquid CO2Static extracting 40min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic
Extraction process parameter is：When temperature is heated to 50 DEG C, pressurize to system, after pressure reaches 50MPa, adjust CO2Flow
For 25L/min, keep constant temperature and pressure to discharge after carrying out cycling extraction 4h, be centrifuged under conditions of 1000r/min
To potato essential oil.
(2) auxiliary material extraction of essential oil：
Step A1：Auxiliary material is puffing：Each raw material is weighed by above-mentioned parts by weight to be crushed respectively, is selected by 50 mesh sieve mesh screens,
Then it is 1 according to solid-liquid mass ratio by ethanol solution that Chinese toon wood skin powder and percentage by volume are 75%:2 mixing；By Radix agastaches
The butanol solution that powder is 10% with percentage by volume is 1 according to solid-liquid mass ratio:1 mixing；By Semen sesami nigrum powder and volume hundred
The ethanol solution that fraction is 75% is 1 according to solid-liquid mass ratio:1 mixing；According to solid-liquid mass ratio it is 1 by leaf of bamboo powder and water:1
Mixing；Then each powder soak is placed in the refrigerator-freezer that temperature is 0 DEG C and refrigerates 26h；Filtering, takes each powder to mix, is put into dish
It is 9MPa that mixed-powder is put into pressure condition after being infiltrated in seed oil, and temperature is that 25min is kept in 120 DEG C of hot wind, pressure release, then
It is 2MPa that mashed potato is put into pressure condition, keeps 20min to complete auxiliary material in the cold wind that temperature is 5 DEG C puffing；
Step A2：Cellulase treatment：By auxiliary materials of the step S1 after puffing and biology enzyme II solution according to solid-liquid mass ratio
For 1:2 mixing immersions, 30min is stood in temperature is 27 DEG C of insulating box, is then concentrated, dry to go moisture removal to obtain auxiliary
Expect dry powder；Wherein, biology enzyme II solution is made of the raw material of following parts by weight：25 parts of pectase, 50 parts of cellulase, 30
The sodium chloride solution that the amylase, 20 parts of aspergillus flavus and 350 parts of mass percents of part are 2%；The enzyme activity of the pectase
For 1500u/g；The enzyme activity of the cellulase is 2000u/g；The enzyme activity of the amylase is 1200u/g；It is described yellow bent
Mould living bacteria count is 6.7 × 108CFU/g；
Step A3：Supercritical CO2Extraction：The auxiliary material dry powder of step S2 is put into extraction kettle, when pressure is 20MPa,
With liquid CO2Static extracting 30min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic extraction technological parameter
For：When temperature is heated to 60 DEG C, pressurize to system, after pressure reaches 40MPa, adjust CO2Flow is 25L/min, is protected
Hold after constant temperature and pressure carries out cycling extraction 5h and discharge, be centrifuged to obtain auxiliary material essential oil under conditions of 1000r/min.
(3) essential oil mixes：
Potato essential oil and auxiliary material essential oil are weighed according to percentage by weight, is 75% by potato essential oil and percentage by volume
Ethanol solution according to 2:1 is mixed, and 7min is stirred under the speed conditions of 800r/min, then adds auxiliary material essential oil,
Continue to stir 8min, rotary evaporation concentration goes moisture removal to be made.
The preparation method of the special consolidant of the present embodiment callus includes the following steps：
(1) each material composition is weighed by weight；
(2) after potato composite essential oil is mixed with the sodium chloride that mass concentration is 2%, it is put into homogenizer and stirs
Emulsion is made, and the pH value of emulsion is adjusted to 7.5, is placed on refrigeration 50min in the refrigerator-freezer that temperature is 0 DEG C and obtains Ma Ling
Potato composite essential oil emulsion, the mixing time are 30min；Speed of agitator is 600r/min；
(3) borax is added into the potato composite essential oil emulsion of step (2), and then pH value is adjusted to 5.9 again
After addition pepper extract, pepper extract and Folium Artemisiae Argyi extract are sufficiently stirred, it is put into Ultrasound Instrument and is ultrasonically treated, ultrasound
The time of processing is 50min, and it is 50 DEG C to be ultrasonically treated temperature, obtains the special consolidant of the callus.
Embodiment 3：
The special consolidant of the present embodiment callus comprises the following components in parts by weight：12 parts of potato composite precision
Oil, 15 parts of pepper extract, 20 parts of pepper extract, 22 parts of Folium Artemisiae Argyi extract, 6 parts of borax and 115 parts of quality
Concentration is 2% sodium chloride solution.
The extraction of the extracting method of pepper extract, the extracting method of pepper extract, Folium Artemisiae Argyi extract in the present embodiment
Method and embodiment 1 are completely the same.
Wherein, the potato essential oil and percentage by weight that potato composite essential oil is 80% by percentage by weight are 20%
Auxiliary material essential oil forms, and the potato essential oil of the present embodiment is made by potato；The auxiliary material essential oil of the present embodiment is by following parts by weight
Component be made：21 parts of Chinese toon wood skin, 21 parts of Radix agastaches, 24 parts of Semen sesami nigrum and 40 parts of the leaf of bamboo.
In the present embodiment, potato composite essential oil preparation method includes potato extraction of essential oil, auxiliary material extraction of essential oil and essence
Three steps of oil mixing are made, and comprise the following steps that：
(1) potato extraction of essential oil：
Step S1：Potato is puffing：Potato is cut into the potato block that thickness is 2mm, then potato block is put into temperature
Spend in the frozen water for 1 DEG C and soak 1.5h, potato block is taken out to drain to be totally submerged in rapeseed oil and is taken out after holding 2s, is put into
Cooked in steamer, mashed into paste, it is 9MPa that mashed potato then is put into pressure condition, and temperature is to be kept in 103 DEG C of hot wind
12min, pressure release, then it is 1MPa that mashed potato is put into pressure condition, keeps 8min to complete potato in the cold wind that temperature is 2 DEG C
It is puffing；
Step S2：Cellulase treatment：By potatos of the step S1 after puffing and biology enzyme I solution according to solid-liquid mass ratio
For 1:2 mixing immersions, 25min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes moisture removal to obtain horse
Bell potato dry powder；Wherein, biology enzyme I solution is made of the raw material of following parts by weight：32 parts of amylase, 22 parts of carbohydrase, 8 parts
Aspergillus oryzae, 15 parts of barbaloin and 270 parts of mass percents be 1% sodium hydroxide solution；The enzyme activity of the amylase
For 900u/g；The enzyme activity of the carbohydrase is 1100u/g；The living bacteria count of the aspergillus oryzae is 4.7 × 107CFU/g；
Step S3：Microwave treatment：After the potato flour of step S2 is cooled to room temperature, it is laid in plate, tiling is thick
Spend for 2mm, be placed on microwave oven turntable and carry out microwave treatment, the frequency of the microwave treatment is 2300MHZ, and power is
1000W, total duration 180s；
Step S4：Supercritical CO2Extraction：By in the potato flour input extraction kettle after step S3 microwave treatments, pressing
When power is 18MPa, with liquid CO2Static extracting 35min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic
Extraction process parameter is：When temperature is heated to 45 DEG C, pressurize to system, after pressure reaches 45MPa, adjust CO2Flow
For 20L/min, keep constant temperature and pressure to discharge after carrying out cycling extraction 3h, be centrifuged to obtain under conditions of 900r/min
Potato essential oil.
(2) auxiliary material extraction of essential oil：
Step A1：Auxiliary material is puffing：Each raw material is weighed by above-mentioned parts by weight to be crushed respectively, is selected by 40 mesh sieve mesh screens,
Then it is 1 according to solid-liquid mass ratio by ethanol solution that Chinese toon wood skin powder and percentage by volume are 75%:2 mixing；By Radix agastaches
The butanol solution that powder is 10% with percentage by volume is 1 according to solid-liquid mass ratio:1 mixing；By Semen sesami nigrum powder and volume hundred
The ethanol solution that fraction is 75% is 1 according to solid-liquid mass ratio:1 mixing；According to solid-liquid mass ratio it is 1 by leaf of bamboo powder and water:1
Mixing；Then each powder soak is placed in the refrigerator-freezer that temperature is -1 DEG C and refrigerates 25h；Filtering, takes each powder to mix, is put into dish
It is 9MPa that mixed-powder is put into pressure condition after being infiltrated in seed oil, and temperature is that 23min is kept in 115 DEG C of hot wind, pressure release, then
It is 1MPa that mashed potato is put into pressure condition, keeps 18min to complete auxiliary material in the cold wind that temperature is 3 DEG C puffing；
Step A2：Cellulase treatment：By auxiliary materials of the step S1 after puffing and biology enzyme II solution according to solid-liquid mass ratio
For 1:1 mixing immersion, 25min is stood in temperature is 27 DEG C of insulating box, is then concentrated, dry to go moisture removal to obtain auxiliary
Expect dry powder；Wherein, biology enzyme II solution is made of the raw material of following parts by weight：20 parts of pectase, 40 parts of cellulase, 25
The sodium chloride solution that the amylase, 15 parts of aspergillus flavus and 330 parts of mass percents of part are 2%；The enzyme activity of the pectase
For 1300u/g；The enzyme activity of the cellulase is 1800u/g；The enzyme activity of the amylase is 1100u/g；It is described yellow bent
Mould living bacteria count is 5.7 × 108CFU/g；
Step A3：Supercritical CO2Extraction：The auxiliary material dry powder of step S2 is put into extraction kettle, when pressure is 17MPa,
With liquid CO2Static extracting 25min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic extraction technological parameter
For：When temperature is heated to 55 DEG C, pressurize to system, after pressure reaches 35MPa, adjust CO2Flow is 20L/min, is protected
Hold after constant temperature and pressure carries out cycling extraction 4h and discharge, be centrifuged to obtain auxiliary material essential oil under conditions of 900r/min.
(3) essential oil mixes：
Potato essential oil and auxiliary material essential oil are weighed according to percentage by weight, is 75% by potato essential oil and percentage by volume
Ethanol solution according to 2:1 is mixed, and 6min is stirred under the speed conditions of 700r/min, then adds auxiliary material essential oil,
Continue to stir 7min, rotary evaporation concentration goes moisture removal to be made.
The preparation method of the special consolidant of the present embodiment callus includes the following steps：
(1) each material composition is weighed by weight；
(2) after potato composite essential oil is mixed with the sodium chloride that mass concentration is 2%, it is put into homogenizer and stirs
Emulsion is made, and the pH value of emulsion is adjusted to 7.0, is placed on refrigeration 40min in the refrigerator-freezer that temperature is -1 DEG C and obtains horse
Bell potato composite essential oil emulsion, the mixing time are 25min；Speed of agitator is 500r/min；
(3) borax is added into the potato composite essential oil emulsion of step (2), and then pH value is adjusted to 5.5 again
After addition pepper extract, pepper extract and Folium Artemisiae Argyi extract are sufficiently stirred, it is put into Ultrasound Instrument and is ultrasonically treated, ultrasound
The time of processing is 45min, and it is 45 DEG C to be ultrasonically treated temperature, obtains the special consolidant of the callus.
(1) the research control experiment of potato composite essential oil characteristic：
Control group 1：
The potato composite essential oil of this control group contains only potato essential oil, without using auxiliary material essential oil, other preparation methods with
Embodiment 1 is identical.
Control group 2：
The potato composite essential oil of this control group contains only auxiliary material essential oil, without potato essential oil, other preparation methods and reality
It is identical to apply example 1.
Control group 3：
The auxiliary material essential oil of this control group potato composite essential oil only contains Radix agastaches, Semen sesami nigrum and several components of the leaf of bamboo, i.e.,
The auxiliary material essence that the potato essential oil and percentage by weight that the potato essential oil of this control group is 70% by percentage by weight are 30%
Oil composition, wherein, potato essential oil is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：13 parts of wrinkled giant hyssop
Leaf, 24 parts of Semen sesami nigrum and 34 parts of leaf of bamboo composition, other preparation methods are identical with embodiment 1.
Control group 4：
The auxiliary material essential oil of this control group potato composite essential oil only contains Chinese toon wood skin, Semen sesami nigrum and several components of the leaf of bamboo, i.e.,
The auxiliary material essence that the potato essential oil and percentage by weight that the potato essential oil of this control group is 70% by percentage by weight are 30%
Oil composition, wherein, potato essential oil is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：17 parts of Chinese toon wood
Skin, 24 parts of Semen sesami nigrum and 34 parts of leaf of bamboo composition, other preparation methods are identical with embodiment 1.
Control group 5：
The auxiliary material essential oil of this control group potato composite essential oil only contains Chinese toon wood skin, Radix agastaches and several components of the leaf of bamboo, i.e.,
The auxiliary material essence that the potato essential oil and percentage by weight that the potato essential oil of this control group is 70% by percentage by weight are 30%
Oil composition, wherein, potato essential oil is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：17 parts of Chinese toon wood
Skin, 13 parts of Radix agastaches and 34 parts of leaf of bamboo composition, other preparation methods are identical with embodiment 1.
Control group 6：
The auxiliary material essential oil of this control group potato composite essential oil only contains Chinese toon wood skin, Radix agastaches and several components of Semen sesami nigrum,
That is the auxiliary material that the potato essential oil and percentage by weight that the potato essential oil of this control group is 70% by percentage by weight are 30%
Essential oil forms, wherein, potato essential oil is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：17 parts of Chinese toon
Wood skin, 13 parts of Radix agastaches and 24 parts of Semen sesami nigrum composition, other preparation methods are identical with embodiment 1.
Control group 7：
Without expanding treatment in the potato essential oil producing process of this control group potato composite essential oil, but directly will
Potato flour is digested, supercritical CO2Extraction, other preparation methods are identical with embodiment 1.
Control group 8：
Without enzymolysis in the potato essential oil producing process of this control group potato composite essential oil, but carry out potato
Puffing, supercritical CO2Extraction, other preparation methods are identical with embodiment 1.
Control group 9：
Only pass through supercritical CO in the potato essential oil producing process of this control group potato composite essential oil2Extraction, it is other
Preparation method is identical with embodiment 1.
Control group 10：
Without expanding treatment in the auxiliary material essential oil producing process of this control group potato composite essential oil, but directly will be auxiliary
Feed powder end is digested, supercritical CO2Extraction, other preparation methods are identical with embodiment 1.
Control group 11：
Without enzymolysis in the auxiliary material essential oil producing process of this control group potato composite essential oil, but carry out auxiliary material powder
Through puffing, supercritical CO2Extraction, other preparation methods are identical with embodiment 1.
Control group 12：
Only pass through supercritical CO in the auxiliary material essential oil producing process of this control group potato composite essential oil2Extraction, other systems
Preparation Method is identical with embodiment 1.
Test experiments 1：
Measure in embodiment 1-3 and control group 1-12, the yield of the potato composite essential oil of preparation, Vitamin C content with
And DPPH radical scavenging activities, concrete condition are shown in Table 1：
Table 1
| Group | Refined oil rate (%) | Vitamin C content (%) | DPPH free radical scavenging activities (%) |
| Embodiment 1 | 78.36 | 18.22 | 87.36 |
| Embodiment 2 | 79.32 | 17.36 | 88.94 |
| Embodiment 3 | 76.35 | 17.31 | 90.16 |
| Control group 1 | 76.52 | 0.22 | 61.03 |
| Control group 2 | 77.48 | 12.36 | 38.26 |
| Control group 3 | 74.51 | 13.02 | 72.36 |
| Control group 4 | 75.26 | 12.03 | 71.02 |
| Control group 5 | 76.11 | 13.21 | 73.06 |
| Control group 6 | 75.03 | 12.02 | 68.26 |
| Control group 7 | 51.02 | 8.36 | 45.61 |
| Control group 8 | 53.21 | 6.32 | 38.56 |
| Control group 9 | 55.31 | 7.25 | 36.14 |
| Control group 10 | 47.26 | 17.03 | 46.98 |
| Control group 11 | 41.06 | 17.22 | 37.68 |
| Control group 12 | 50.44 | 17.30 | 34.16 |
As seen from the above table, the refined oil rate of embodiment 1-3 is suitable with control group 1-6 higher than control group 7-12, illustrates this Shen
Please puffing, biological enzymolysis, supercritical CO in method of extraction of essential oil2Extraction phase combination can effectively improve refined oil rate：Embodiment 1-3
In ascorbic content apparently higher than control group 1, illustrate to contain more rich vitamin C component in potato essential oil；Embodiment
The Vitamin C content of 1-3 apparently higher than control group 7-9 and control group 10-12, illustrate in potato method of extraction of essential oil it is puffing,
Biological enzymolysis, supercritical CO2Extraction phase combination can effectively improve Vitamin C content；The DPPH free radical scavenging activities of embodiment 1-3
Apparently higher than control group 1-12, illustrate that the essential oil of the application and method of extraction of essential oil can effectively remove the raising of DPPH free radicals
The antioxygen rate of essential oil.
Test experiments 2：
Measure in embodiment 1 and control group 1-12, the oily substance component content of potato composite essential oil, concrete condition is shown in
Table 2：
Table 2
| Group | Terpenoid (%) | Ketone compounds (%) | Ester type compound (%) | Hydrocarbon compound (%) | Oxygenatedchemicals (%) |
| Embodiment 1 | 16.25 | 9.36 | 54.36 | 11.06 | 8.97 |
| Control group 1 | 17.26 | 11.36 | 48.36 | 13.25 | 9.77 |
| Control group 2 | 19.40 | 46.35 | 13.25 | 12.36 | 8.64 |
| Control group 3 | 23.02 | 16.25 | 47.15 | 8.25 | 5.33 |
| Control group 4 | 21.25 | 17.26 | 46.29 | 5.36 | 9.84 |
| Control group 5 | 26.35 | 17.06 | 45.98 | 9.36 | 1.25 |
| Control group 6 | 24.25 | 16.34 | 46.09 | 10.03 | 3.29 |
| Control group 7 | 26.35 | 16.97 | 31.02 | 8.64 | 17.02 |
| Control group 8 | 24.19 | 17.36 | 33.06 | 9.13 | 16.26 |
| Control group 9 | 23.14 | 17.09 | 31.02 | 11.31 | 17.44 |
| Control group 10 | 26.35 | 16.88 | 32.54 | 9.26 | 14.97 |
| Control group 11 | 28.35 | 16.46 | 17.25 | 23.25 | 14.69 |
| Control group 12 | 30.25 | 14.28 | 13.25 | 24.26 | 17.96 |
As seen from the above table, the ester type compound component of embodiment 1 is above control group 1-12, and ketone compounds component is low
In control group 1-12, illustrate, the ester-containing amount of potato essential oil of the application is high, and amount containing ketone is low, illustrates the potato essential oil of the application
Its fragrance does not have other control groups to be pungent.
(2) the special consolidant characteristic test experiment of callus：
Test experiments：Consolidant according to the configuration of following test group is applied to the callus tissue culture in Banana Tissue culture
Culture medium in, in the culture medium applied to the callus tissue culture in guava tissue cultures, applied to soaking grapes cuttage
The grape branch of process clip, and the 5d rooting rates of callus and cuttage branch, 5d root longs, 30d survival rates are measured, it is specifically shown in
Table 3：
Test group 1：Consolidant is produced according to the method for embodiment 1.
Test group 2：Consolidant is configured according to following component：11 parts of the potato composite essential oil (potato of this test group
Composite essential oil is free of auxiliary material derived essential oil, is only made of potato essential oil), 12 parts of pepper extract, the extraction of 15 parts of Chinese prickly ash
Thing, 20 parts of Folium Artemisiae Argyi extract, 5 parts of borax and 110 parts of mass concentration are 2% sodium chloride solution.Other preparation methods
It is completely the same with embodiment 1.
Test group 3：Consolidant is configured according to following component：12 parts of pepper extract, 15 parts of pepper extract, 20 parts
Folium Artemisiae Argyi extract, 5 parts of borax and 110 parts of mass concentration be 2% sodium chloride solution.Other preparation methods and embodiment
1 is completely the same.
Test group 4：Consolidant is configured according to following component：11 parts of potato composite essential oil, 15 parts of pepper extract,
20 parts of Folium Artemisiae Argyi extract, 5 parts of borax and 110 parts of mass concentration are 2% sodium chloride solution.Other preparation methods and reality
It is completely the same to apply example 1.
Test group 5：Consolidant is configured according to following component：11 parts of potato composite essential oil, 12 parts of pepper extract,
20 parts of Folium Artemisiae Argyi extract, 5 parts of borax and 110 parts of mass concentration are 2% sodium chloride solution.Other preparation methods and reality
It is completely the same to apply example 1.
Test group 6：Consolidant is configured according to following component：11 parts of potato composite essential oil, 12 parts of pepper extract,
15 parts of pepper extract, 5 parts of borax and 110 parts of mass concentration are 2% sodium chloride solution.Other preparation methods and reality
It is completely the same to apply example 1.
Test group 7：Consolidant is configured according to following component：11 parts of potato composite essential oil, 12 parts of pepper extract,
15 parts of pepper extract, 20 parts of Folium Artemisiae Argyi extract and 110 parts of mass concentration are 2% sodium chloride solution.Other are prepared
Method and embodiment 1 are completely the same.
Control group：Without using any consolidant.
Table 3
As seen from the above table, the 5d rooting rates of test group, 5d root longs, 30d survival rates are all higher than test group 2-7, more than control
Group, illustrate using the consolidant of the application can effectively improve the rooting rate of tissue culture and cuttage seeding, root long and into
Motility rate.
In conclusion being prepared using the method for the present invention, potato composite essential oil can improve refined oil rate, raising essential oil resists
Oxidability, and its fragrance is more comfortable；Meanwhile being cured for plants wound can be promoted using potato composite essential oil in consolidant
Close, so as to lift the rooting rate of callus and cuttage seeding, root long and survival rate.
Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (7)
1. a kind of special consolidant of callus, it is characterised in that the special consolidant comprises the following components in parts by weight：
11 parts -13 parts of potato composite essential oil, 12 parts -18 parts of pepper extract, 15 parts -25 parts of pepper extract, 20 part -25
The Folium Artemisiae Argyi extract, 5 parts -7 parts of borax and 110 parts -120 parts of mass concentration of part are 2% sodium chloride solution.
2. the special consolidant of callus according to claim 1, it is characterised in that the potato composite essential oil is by weight
Measure the potato essential oil that percentage is 70%-90% and the auxiliary material essential oil composition that percentage by weight is 10%-30%.
3. the special consolidant of callus according to claim 2, it is characterised in that the potato essential oil is by potato
It is made；The auxiliary material essential oil is made by the component of following parts by weight：17 parts -29 parts of Chinese toon wood skin, 13 parts -31 parts of Radix agastaches,
24 parts -32 parts of Semen sesami nigrum and 34 parts -46 parts of the leaf of bamboo.
4. the special consolidant of callus according to claim 2, it is characterised in that prepared by the potato composite essential oil
Method includes potato extraction of essential oil, three steps of auxiliary material extraction of essential oil and essential oil mixing are made.
5. the special consolidant of callus according to claim 4, it is characterised in that the potato extraction of essential oil passes through
Potato is puffing, cellulase treatment, microwave treatment and supercritical CO2Four steps of extraction obtain；The cellulase treatment uses
Biology enzyme I solution be made of the raw material of following parts by weight：30 parts -35 parts of amylase, 20 parts -25 parts of carbohydrase, 5 parts -
The sodium hydroxide solution that 10 parts of aspergillus oryzae, 10 parts -20 parts of barbaloin and 250 parts of -300 parts of mass percents are 1%；It is described
The enzyme activity of amylase is 800u/g-1000u/g；The enzyme activity of the carbohydrase is 1000u/g-1200u/g；The aspergillus oryzae
Living bacteria count be 3.1 × 107~6.7 × 107CFU/g。
6. the special consolidant of callus according to claim 4, it is characterised in that the auxiliary material extraction of essential oil is by auxiliary
Expect puffing, cellulase treatment and supercritical CO2Four steps of extraction obtain；The biology enzyme II solution that the cellulase treatment uses
It is made of the raw material of following parts by weight：15 parts -25 parts of pectase, 30 parts -50 parts of cellulase, 20 parts -30 parts of starch
The sodium chloride solution that enzyme, 10 parts -20 parts of aspergillus flavus and 300 parts of -350 parts of mass percents are 2%；The enzyme activity of the pectase
Power is 1200u/g-1500u/g；The enzyme activity of the cellulase is 1500u/g-2000u/g；The enzyme activity of the amylase
For 900u/g-1200u/g；The living bacteria count of the aspergillus flavus is 3.9 × 108~6.7 × 108CFU/g。
7. a kind of preparation method for preparing the special consolidant of any one callus as described in claim 1-6, its feature exist
In described method includes following steps：
(1) each material composition is weighed by weight；
(2) after potato composite essential oil is mixed with the sodium chloride that mass concentration is 2%, it is put into stirring in homogenizer and is made
Emulsion, and the pH value of emulsion is adjusted to 6.8-7.5, it is placed in the refrigerator-freezer that temperature is -2 DEG C~0 DEG C and refrigerates 30min-
50min obtains potato composite essential oil emulsion, and the mixing time is 20min-30min；Speed of agitator is 400r/min-
600r/min；
(3) borax is added into the potato composite essential oil emulsion of step (2), and then pH value is adjusted to 5.2-5.9 again
After addition pepper extract, pepper extract and Folium Artemisiae Argyi extract are sufficiently stirred, it is put into Ultrasound Instrument and is ultrasonically treated, ultrasound
The time of processing is 40min-50min, and it is 40 DEG C~50 DEG C to be ultrasonically treated temperature, obtains the special consolidant of the callus.
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| CN112806393A (en) * | 2021-02-04 | 2021-05-18 | 朝阳康泉纳米材料有限公司 | Traditional Chinese medicine extract for preventing and treating plant bacterial diseases and application thereof |
| CN114557205A (en) * | 2022-04-12 | 2022-05-31 | 云南省农业科学院茶叶研究所 | Method for improving grafting rapid propagation survival rate of tea tree germplasm resources |
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