CN106927944A - It is a kind of to promote consolidant of longan grafting wound healing and preparation method thereof - Google Patents
It is a kind of to promote consolidant of longan grafting wound healing and preparation method thereof Download PDFInfo
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- CN106927944A CN106927944A CN201710052942.8A CN201710052942A CN106927944A CN 106927944 A CN106927944 A CN 106927944A CN 201710052942 A CN201710052942 A CN 201710052942A CN 106927944 A CN106927944 A CN 106927944A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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Abstract
The present invention relates to fruit tree cultivation technical field, more particularly to a kind of to promote consolidant of longan grafting wound healing and preparation method thereof, healing agent prescription of the invention repairs composition including liquid and solid repairs composition；Liquid repairs composition and is mainly the active component extracted from plant, wherein, these plants include cactus, the red sage root, flaccid knotweed herb, Lysimachia foenum-graecum, felwort and ganoderma lucidum；These liquid components derive from plant source, without toxicity, do not endanger ecological environment；Solid of the invention repairs composition to be included：Analysis for soybean powder, tapioca, bean straw powder end and bone meal these compositions provide abundant nutrition can to grafting growth；Consolidant of the invention is also prepared into paste simultaneously, can form biomembrane in the wound of grafting, makes the drug effect of consolidant and has slow releasing function, the wound healing of promotion grafting, the effect of antibacterial that can be long-acting.
Description
【Technical field】
The present invention relates to fruit tree cultivation technical field, more particularly to a kind of consolidant of promotion longan grafting wound healing
And preparation method thereof.
【Background technology】
Longan is a kind of subtropical fruit, and the age of tree is long, and strain yield is high, is good economic fruit, but longan is suitable
Answering property is not strong, and in order to strengthen the adaptability of tree-longan, Planting household carries out nursery to longan and changes often through the mode of grafting
Enter, plant graft needs to connect with another plant again by grafting knife destruction plant, during this, due to plant
Wound is formd, after often facing Railway Project (1) plant injury, it may occur that defense reaction, if grafting and anvil
Wooden affinity is remote, it will cause the stock can not to cause rootstock seedling depauperation effectively by nutriment transport in grafting
It is even dead；(2) after stock and grafting form wound, easy breed bacteria and attract worm ant, nursery stock can be accelerated dead.
【The content of the invention】
In view of the above, it is necessary to provide a kind of consolidant for promoting longan grafting wound healing and its preparation side
Method, can accelerate the wound healing of grafting, prevent bacterium infection, make stock shoot survival percent high, long slightly fast, promote the life of rootstock seedling
It is long.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
A kind of consolidant of promotion longan grafting wound healing, including liquid repairs composition and solid repairs composition, institute
It is 30-40 to state liquid and repair composition and the ratio of solid reparation composition:10-13；
The liquid is repaired composition and is made up of the raw material of following weight portion：
36 parts -49 parts of cactus extraction, 14 parts -32 parts of red sage root extract, 11 parts -18 parts of flaccid knotweed herb are extracted
Liquid, 12 parts -29 parts of Lysimachia foenum-graecum extract solution, 19 parts -28 parts of felwort extract solution and 23 parts -31 parts of lucidum extracting liquid；
The solid is repaired composition and is made up of the raw material of following weight portion：
8 parts -15 parts of analysis for soybean powder, 18 parts -29 parts of tapioca, 16 parts -24 parts of bean straw powder end and 7 parts -15 parts
Bone meal.
Further, the cactus extraction preparation method is：By fresh cactus deburring, smash to pieces, be subsequently adding same
Percentage by volume etc. mass parts is filtered off to be crossed after the ethyl acetate solution refluxing extraction 2h-3h of 40% (v/v) -50% (v/v)
Except filter residue obtains filtrate, filtrate is carried out into vacuum distillation and removes ethyl acetate, then concentrated, be concentrated to concentrate for stoste
1/4 stopping of volume is concentrated to give cactus extraction.
Further, the red sage root extract preparation method is：By moisture content for the red sage root of 5%-10% is cut into slices, Ran Houjia
Enter 3-5 times of mass parts ethanol volumn concentration to be soaked in the yellow rice wine of 60% (v/v) -70% (v/v), after 30d-35d,
Danshen Tablets are poured into marmite together with yellow rice wine, infusion are carried out with slow fire after boiling, cooled down after 2h-3h, filter removal filter residue,
Filtrate is proceeded into concentration, until concentrate for 1/3 stopping of stoste volume is concentrated to give red sage root extract.
Further, the flaccid knotweed herb extraction liquid and preparation method thereof is：The fresh whole strain of flaccid knotweed herb is ground, Ran Houjia
Enter 2-3 times of mass parts water boil after turn slow fire and carry out infusion, flaccid knotweed herb extract solution is filtrated to get after infusion 3h-4h.
Further, the Lysimachia foenum-graecum extraction liquid and preparation method thereof is：Fresh Lysimachia foenum-graecum blade is won, 2- is added after shredding
4 times of percentage by volumes of mass parts are filtered to filter removal after the butanol solution refluxing extraction 2h-3h of 20% (v/v) -30% (v/v)
Slag obtains filtrate, and filtrate is carried out into vacuum distillation removes butanol, is then concentrated, and it is the 1/ of stoste volume to be concentrated to concentrate
5 stoppings are concentrated to give Lysimachia foenum-graecum extract solution.
Further, the felwort extraction liquid and preparation method thereof is：By moisture content for the felwort blade of 5%-10% is cut
It is broken, it is subsequently adding the acetone soln refluxing extraction 1h- that the 3-5 times of percentage by volume of mass parts is 10% (v/v) -20% (v/v)
Removal filter residue is filtered after 2h and obtains filtrate, filtrate is carried out into vacuum distillation removes acetone, is then concentrated to concentrate for primary liquid
1/2 long-pending stopping is concentrated to give felwort extract solution.
Further, the lucidum extracting liquid preparation method is：Whole strain is put after moisture content is cleaned for the ganoderma lucidum of 2%-5%
Immersion 30d-40d then, will in entering the ethanol solution that the 5-6 times of percentage by volume of mass parts is 70% (v/v) -80% (v/v)
Ganoderma lucidum pours into after carrying out refluxing extraction 1h-2h in return tank together with ethanol solution, is then filtered, and filtrate is depressurized
Ethanol is distilled off, then extraction raffinate is concentrated, until concentrate stops being concentrated to give ganoderma lucidum extraction for 1/5 of stoste volume
Liquid.
Further, the consolidant preparation method of described promotion longan grafting wound healing comprises the following steps：
(1) by the weight portion weigh cactus extraction, red sage root extract, flaccid knotweed herb extract solution, Lysimachia foenum-graecum extract solution,
Felwort extract solution and lucidum extracting liquid, then carry out being mixed to get liquid reparation constituents mixt；
(2) carry out being mixed to get solid after weighing celestial analysis for soybean powder, tapioca, bean straw powder end and bone meal by the weight portion
Mixture, is then processed into the powder that granularity is 200-300 mesh by solid mixture, obtains solid and repairs constituents mixt；
(3) liquid of step (1) is repaired into constituents mixt and repairs constituents mixt by described heavy with the solid of step (2)
After amount is than uniform mixing, the vaseline of liquid is added, be adjusted to paste and obtain consolidant.
The present invention has the advantages that：
(1) healing agent prescription of the invention includes that liquid repairs composition and solid repairs composition；It is main that liquid repairs composition
Be from plant extract active component, wherein, these plants include cactus, the red sage root, flaccid knotweed herb, Lysimachia foenum-graecum, felwort and
Ganoderma lucidum；Cactus extraction mainly takes from the fleshy stem of cactus, by containing abundant celestial being after ethyl acetate solution extraction
The palm polysaccharide and several amino acids and flavones, with the effect for repairing, improving cell differentiation；Red sage root extract is mainly taken from
The stem root of the red sage root, immersion by yellow rice wine solution and refluxing extraction after drying, can separate out flavonoids, triterpenes, sterol etc. into
Point, the effect that these compositions have bactericidal antiphlogistic, reparation, improve cell differentiation；Flaccid knotweed herb extract solution is mainly taken from fresh
The steaming strain of flaccid knotweed herb, by fully separating out active component therein after water infusion, there is polygonum flaccidum plant desinsection, food refusal, repellent to live
Performance effectively improves the sterilization of consolidant, anthelmintic action；Lysimachia foenum-graecum has the plain aromatic oil of similar tonka-bean, the work with expelling parasite in itself
With the fragrance of plain aromatic oil is denseer after butanol refluxing extraction, is more easy to play anthelmintic action；Felwort extract solution is mainly taken from dry
Dry felwort blade, by after acetone extraction, its gentiopicrin, Swertiamarin, sweroside, trilobatin, amarogentin, tetrem
Acyl gentiopicrin, amaroswerin；The active ingredients such as gentioflavine, erythricine, cupreol are fully enriched with, withered to knurl bacterium
The inhibitory action such as careless bacillus, Agrobacterium tumefaciens；Plant can be protected not receive pest and disease damage；The ganoderma lucidum that lucidum extracting liquid is mainly taken from
Whole strain, ganoderma lucidum soaked by high concentration ethanol, after refluxing extraction, wherein containing abundant GL-B, can play antioxygen
Change is acted on, and can promote the healing of grafting wound；In sum, formula of the invention derives from plant source, without toxicity, no
Harm ecological environment, while raising grafting immunity can also be played, accelerates wound healing, sterilization, sterilization, the effect of expelling parasite.
(2) solid of the invention is repaired composition and is made up of following raw material：Analysis for soybean powder, tapioca, bean straw powder end and bone meal；
Wherein, contain abundant nitrogen source and trace element in analysis for soybean powder and bean straw powder, supplement nutrient can to grafting, tapioca is to form sediment
Powder class material, and oxidation of the air to wound can be completely cut off with film forming, abundant phosphorus and trace element, energy are contained in bone meal
Promote the plant life of grafting, consolidant of the invention is also prepared into paste, can form biomembrane in the wound of grafting, makes to heal
The drug effect of mixture has slow releasing function, the wound healing of promotion grafting, antibacterial that can be long-acting.
【Specific embodiment】
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine by any way.
Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated otherwise, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1：
Healing agent prescription and preparation method including embodiment：
1st, the healing agent prescription of the present embodiment：
Consolidant includes that liquid repairs composition and solid repairs composition, wherein, liquid repairs composition and repairs composition with solid
Ratio be 30:10
(1) liquid is repaired composition and is made up of the raw material of following weight portion：36 parts of cactus extraction, 14 parts of the red sage root are carried
Liquid, 11 parts of flaccid knotweed herb extract solution, 12 parts of Lysimachia foenum-graecum extract solution, 19 parts of felwort extract solution and 23 parts of ganoderma lucidum is taken to extract
Liquid；
Wherein, people's palm extraction liquid and preparation method thereof is：By fresh cactus deburring, smash to pieces, be subsequently adding equal quality part
Percentage by volume obtains filtrate to filter removal filter residue after the ethyl acetate solution refluxing extraction 2h of 40% (v/v), and filtrate is entered
Row vacuum distillation removes ethyl acetate, is then concentrated, and is concentrated to 1/4 stopping that concentrate is stoste volume and is concentrated to give
Cactus extraction；
Wherein, red sage root extract preparation method is：By the red sage root section that moisture content is 5%, 3 times of mass parts second are subsequently adding
Danshen Tablets after 30d, are poured into marmite by alcohol volumn concentration to be soaked in the yellow rice wine of 60% (v/v) together with yellow rice wine
It is interior, infusion is carried out with slow fire after boiling, cooled down after 2h, filter removal filter residue, filtrate is proceeded into concentration, until concentrate is
1/3 stopping of stoste volume is concentrated to give red sage root extract；
Wherein, flaccid knotweed herb extraction liquid and preparation method thereof is：The fresh whole strain of flaccid knotweed herb is ground, 2 times of matter are subsequently adding
The water of amount part turns slow fire after boiling carries out infusion, and flaccid knotweed herb extract solution is filtrated to get after infusion 3h；
Wherein, Lysimachia foenum-graecum extraction liquid and preparation method thereof is：Fresh Lysimachia foenum-graecum blade is won, 2 times of mass parts are added after shredding
Percentage by volume for 20% (v/v) butanol solution refluxing extraction 2h after filter removal filter residue obtain filtrate, filtrate is carried out
Vacuum distillation removes butanol, is then concentrated, and is concentrated to 1/5 stopping that concentrate is stoste volume and is concentrated to give Lysimachia foenum-graecum
Extract solution；
Wherein, felwort extraction liquid and preparation method thereof is：The felwort blade that moisture content is 5% is shredded, 3 times are subsequently adding
The percentage by volume of mass parts obtains filtrate to filter removal filter residue after the acetone soln refluxing extraction 1h of 10% (v/v), will filter
Liquid carries out vacuum distillation and removes acetone, is then concentrated to concentrate and is carried for 1/2 stopping of stoste volume is concentrated to give felwort
Take liquid；
Wherein, lucidum extracting liquid preparation method is：Whole strain is put into 5 times of mass parts after the ganoderma lucidum that moisture content is 2% is cleaned
Percentage by volume for 70% (v/v) ethanol solution in soak 30d then, ganoderma lucidum is poured into return tank together with ethanol solution
After inside carrying out refluxing extraction 1h, then filtered, filtrate is carried out into vacuum distillation removes ethanol, then extraction raffinate is concentrated,
Until concentrate for 1/5 stopping of stoste volume is concentrated to give lucidum extracting liquid；
(2) solid is repaired composition and is made up of the raw material of following weight portion：8 parts of analysis for soybean powder, 18 parts of tapioca, 16 parts
Bean straw powder end and 7 parts of bone meal.
2nd, the consolidant preparation method of the present embodiment：
The method comprises the following steps：
(1) cactus extraction, red sage root extract, flaccid knotweed herb extract solution, Lysimachia foenum-graecum is weighed by the weight portion of formula to extract
Liquid, felwort extract solution and lucidum extracting liquid, then carry out being mixed to get liquid reparation constituents mixt；
(2) after weighing celestial analysis for soybean powder, tapioca, bean straw powder end and bone meal by the weight portion of formula be mixed to get admittedly
Body mixture, is then processed into the powder that granularity is 200 by solid mixture, obtains solid and repairs constituents mixt；
(3) liquid of step (1) is repaired into constituents mixt and repairs constituents mixt by weight with the solid of step (2)
For：35:After 12 uniform mixing, the vaseline of liquid is added, be adjusted to paste and obtain consolidant.
Embodiment 2：
Healing agent prescription and preparation method including embodiment：
1st, the healing agent prescription of the present embodiment：
Consolidant includes that liquid repairs composition and solid repairs composition, wherein, liquid repairs composition and repairs composition with solid
Ratio be 40:13
(1) liquid is repaired composition and is made up of the raw material of following weight portion：49 parts of cactus extraction, 32 parts of the red sage root are carried
Liquid, 18 parts of flaccid knotweed herb extract solution, 29 parts of Lysimachia foenum-graecum extract solution, 28 parts of felwort extract solution and 31 parts of ganoderma lucidum is taken to extract
Liquid；
Wherein, people's palm extraction liquid and preparation method thereof is：By fresh cactus deburring, smash to pieces, be subsequently adding equal quality part
Percentage by volume obtains filtrate to filter removal filter residue after the ethyl acetate solution refluxing extraction 3h of 50% (v/v), and filtrate is entered
Row vacuum distillation removes ethyl acetate, is then concentrated, and is concentrated to 1/4 stopping that concentrate is stoste volume and is concentrated to give
Cactus extraction；
Wherein, red sage root extract preparation method is：By the red sage root section that moisture content is 10%, 5 times of mass parts are subsequently adding
Danshen Tablets after 35d, are poured into sand by ethanol volumn concentration to be soaked in the yellow rice wine of 70% (v/v) together with yellow rice wine
In pot, infusion is carried out with slow fire after boiling, cooled down after 3h, filter removal filter residue, filtrate is proceeded into concentration, until concentrate
For 1/3 stopping of stoste volume is concentrated to give red sage root extract；
Wherein, flaccid knotweed herb extraction liquid and preparation method thereof is：The fresh whole strain of flaccid knotweed herb is ground, 3 times of matter are subsequently adding
The water of amount part turns slow fire after boiling carries out infusion, and flaccid knotweed herb extract solution is filtrated to get after infusion 4h；
Wherein, Lysimachia foenum-graecum extraction liquid and preparation method thereof is：Fresh Lysimachia foenum-graecum blade is won, 4 times of mass parts are added after shredding
Percentage by volume for 30% (v/v) butanol solution refluxing extraction 3h after filter removal filter residue obtain filtrate, filtrate is carried out
Vacuum distillation removes butanol, is then concentrated, and is concentrated to 1/5 stopping that concentrate is stoste volume and is concentrated to give Lysimachia foenum-graecum
Extract solution；
Wherein, felwort extraction liquid and preparation method thereof is：The felwort blade that moisture content is 10% is shredded, 5 are subsequently adding
The percentage by volume of times mass parts obtains filtrate to filter removal filter residue after the acetone soln refluxing extraction 2h of 20% (v/v), will
Filtrate carries out vacuum distillation and removes acetone, is then concentrated to 1/2 stopping that concentrate is stoste volume and is concentrated to give felwort
Extract solution；
Wherein, lucidum extracting liquid preparation method is：Whole strain is put into 6 times of mass parts after the ganoderma lucidum that moisture content is 5% is cleaned
Percentage by volume for 80% (v/v) ethanol solution in soak 40d then, ganoderma lucidum is poured into return tank together with ethanol solution
After inside carrying out refluxing extraction 2h, then filtered, filtrate is carried out into vacuum distillation removes ethanol, then extraction raffinate is concentrated,
Until concentrate for 1/5 stopping of stoste volume is concentrated to give lucidum extracting liquid；
(2) solid is repaired composition and is made up of the raw material of following weight portion：15 parts of analysis for soybean powder, 29 parts of tapioca, 24
The bean straw powder end of part and 15 parts of bone meal.
2nd, the consolidant preparation method of the present embodiment：
The method comprises the following steps：
(1) cactus extraction, red sage root extract, flaccid knotweed herb extract solution, Lysimachia foenum-graecum is weighed by the weight portion of formula to extract
Liquid, felwort extract solution and lucidum extracting liquid, then carry out being mixed to get liquid reparation constituents mixt；
(2) after weighing celestial analysis for soybean powder, tapioca, bean straw powder end and bone meal by the weight portion of formula be mixed to get admittedly
Body mixture, is then processed into the powder that granularity is 300 mesh by solid mixture, obtains solid and repairs constituents mixt；
(3) liquid of step (1) is repaired into constituents mixt and repairs constituents mixt by weight with the solid of step (2)
For：35:After 12 uniform mixing, the vaseline of liquid is added, be adjusted to paste and obtain consolidant.
Embodiment 3：
Healing agent prescription and preparation method including embodiment：
1st, the healing agent prescription of the present embodiment：
Consolidant includes that liquid repairs composition and solid repairs composition, wherein, liquid repairs composition and repairs composition with solid
Ratio be 35:12
(1) liquid is repaired composition and is made up of the raw material of following weight portion：40 parts of cactus extraction, 20 parts of the red sage root are carried
Liquid, 15 parts of flaccid knotweed herb extract solution, 21 parts of Lysimachia foenum-graecum extract solution, 23 parts of felwort extract solution and 27 parts of ganoderma lucidum is taken to extract
Liquid；
Wherein, people's palm extraction liquid and preparation method thereof is：By fresh cactus deburring, smash to pieces, be subsequently adding equal quality part
Percentage by volume obtains filtrate to filter removal filter residue after the ethyl acetate solution refluxing extraction 2.5h of 45% (v/v), by filtrate
Carry out vacuum distillation and remove ethyl acetate, then concentrated, be concentrated to 1/4 stopping that concentrate is stoste volume and be concentrated to give
To cactus extraction；
Wherein, red sage root extract preparation method is：By the red sage root section that moisture content is 8%, 4 times of mass parts second are subsequently adding
Danshen Tablets after 33d, are poured into marmite by alcohol volumn concentration to be soaked in the yellow rice wine of 65% (v/v) together with yellow rice wine
It is interior, infusion is carried out with slow fire after boiling, cooled down after 2.5h, filter removal filter residue, filtrate is proceeded into concentration, until concentrate
For 1/3 stopping of stoste volume is concentrated to give red sage root extract；
Wherein, flaccid knotweed herb extraction liquid and preparation method thereof is：The fresh whole strain of flaccid knotweed herb is ground, 2-3 times is subsequently adding
The water of mass parts turns slow fire after boiling carry out infusion, and flaccid knotweed herb extract solution is filtrated to get after infusion 3h-4h；
Wherein, Lysimachia foenum-graecum extraction liquid and preparation method thereof is：Fresh Lysimachia foenum-graecum blade is won, 3 times of mass parts are added after shredding
Percentage by volume for 25% (v/v) butanol solution refluxing extraction 2.5h after filter removal filter residue obtain filtrate, filtrate is entered
Row vacuum distillation removes butanol, is then concentrated, and is concentrated to 1/5 that concentrate is stoste volume and stops being concentrated to give clever perfume
Careless extract solution；
Wherein, felwort extraction liquid and preparation method thereof is：The felwort blade that moisture content is 8% is shredded, 4 times are subsequently adding
The percentage by volume of mass parts obtains filtrate to filter removal filter residue after the acetone soln refluxing extraction 1.5h of 15% (v/v), will
Filtrate carries out vacuum distillation and removes acetone, is then concentrated to 1/2 stopping that concentrate is stoste volume and is concentrated to give felwort
Extract solution；
Wherein, lucidum extracting liquid preparation method is：Whole strain is put into 6 times of mass parts after the ganoderma lucidum that moisture content is 3% is cleaned
Percentage by volume for 75% (v/v) ethanol solution in soak 35d then, ganoderma lucidum is poured into return tank together with ethanol solution
After inside carrying out refluxing extraction 1.5h, then filtered, filtrate is carried out into vacuum distillation removes ethanol, then extraction raffinate is carried out dense
Contracting, until concentrate for 1/5 stopping of stoste volume is concentrated to give lucidum extracting liquid；
(2) solid is repaired composition and is made up of the raw material of following weight portion：12 parts of analysis for soybean powder, 23 parts of tapioca, 20
The bean straw powder end of part and 12 parts of bone meal.
2nd, the consolidant preparation method of the present embodiment：
The method comprises the following steps：
(1) cactus extraction, red sage root extract, flaccid knotweed herb extract solution, Lysimachia foenum-graecum is weighed by the weight portion of formula to extract
Liquid, felwort extract solution and lucidum extracting liquid, then carry out being mixed to get liquid reparation constituents mixt；
(2) after weighing celestial analysis for soybean powder, tapioca, bean straw powder end and bone meal by the weight portion of formula be mixed to get admittedly
Body mixture, is then processed into the powder that granularity is 250 mesh by solid mixture, obtains solid and repairs constituents mixt；
(3) liquid of step (1) is repaired into constituents mixt and repairs constituents mixt by weight with the solid of step (2)
For：35:After 12 uniform mixing, the vaseline of liquid is added, be adjusted to paste and obtain consolidant.
Control group 1：
The preparation method of this control group consolidant is carried out in strict accordance with embodiment 1, but does not use solid to repair in formula
Composition.
Control group 2：
The preparation method of this control group consolidant is carried out in strict accordance with embodiment 1, but does not use liquid to repair in formula
Composition.
Control group 3：
The preparation method of this control group consolidant is carried out in strict accordance with embodiment 1, but liquid repairs composition only in formula
Use cactus extraction and red sage root extract.
Control group 4：
The preparation method of this control group consolidant is carried out in strict accordance with embodiment 1, but liquid repairs composition not in formula
Use cactus extraction and red sage root extract.
Control group 5：
The preparation method of this control group consolidant is carried out in strict accordance with embodiment 1, but liquid repairs composition not in formula
Use flaccid knotweed herb extract solution, Lysimachia foenum-graecum extract solution and felwort extract solution.
Control group 6：
The preparation method of this control group consolidant is carried out in strict accordance with embodiment 1, but liquid repairs composition not in formula
Use lucidum extracting liquid.
Control group 7：
The formula of this control group consolidant is carried out in strict accordance with embodiment 1, but is not prepared into paste, and liquid is repaired into
Divide to be repaired after composition mixes with solid and used directly as consolidant.
Checking test：
Carried out in the Experimental Base of our company, choose the rootstock seedling 1000 that size is basically identical, physiological situation is good, healthy and strong
Strain, is divided into 10 groups, every group 100 plants, respectively at after corresponding grafting twig grafting, by embodiment 1-3's and control group 1-7
Consolidant is coated on the grafting wound of rootstock seedling and grafting respectively, and bondage, Ran Houzai are carried out to wound with the polybag of black
Grafting is cultivated under equal conditions, statistics grafting 10d, 20d, 30d survive situation, and measurement 30d's is new
Tip length, opens black plastic bag to 30d, observes the shape and smoothness of grafting wound, and concrete condition is shown in Table 1：
The grafting shoot survival percent of table 1 and wound characterize table：
As seen from the above table, the 10d of embodiment 1-3 survives the quantity of grafting apparently higher than control group 2 and control group 4, with
Control group 1, control group 3, control group 5-7 difference are little；To 20d and 30d control groups 1-7 survive nursery stock number it is obvious under
Drop；Illustrate using the survival rate for being formulated and grafting being significantly improved with preparation method of the invention, and the time ratio for maintaining
Conventional meanses will be grown；In terms of interface controlled observation, the interface of control group 2 and control group 6 is all relatively rough, illustrates that ganoderma lucidum is extracted
Liquid energy repairs grafting interface well, and control group 2, control group 5, control group 6 interface all occur and fester, and illustrates that flaccid knotweed herb is extracted
Liquid, Lysimachia foenum-graecum extract solution and felwort extract solution have good bactericidal action, have slow releasing function after being processed into paste and smearing
Effect can just manifest, and to the new-tip length of 30d embodiments 1-3 apparently higher than control group 1-7, the young sprout of wherein control group 1 is long
Degree is most short, illustrates that solid repairs composition and can be obviously promoted the young sprout growth of grafting.
In sum, prepare consolidant and can greatly improve surviving for grafting using formula of the invention and preparation method
Rate, accelerates the growth of grafting young sprout, promotes wound healing.
Embodiment described above only expresses several embodiments of the invention, and its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection model of the invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (8)
1. a kind of consolidant of promotion longan grafting wound healing, including liquid repairs composition and solid reparation composition, and it is special
Levy and be, it is 30-40 that the liquid repairs composition and the ratio of solid reparation composition:10-13；
The liquid is repaired composition and is made up of the raw material of following weight portion：
36 parts -49 parts of cactus extraction, 14 parts -32 parts of red sage root extract, 11 parts -18 parts of flaccid knotweed herb extract solution, 12
- 29 parts of Lysimachia foenum-graecum extract solution of part, 19 parts -28 parts of felwort extract solution and 23 parts -31 parts of lucidum extracting liquid；
The solid is repaired composition and is made up of the raw material of following weight portion：
8 parts -15 parts of analysis for soybean powder, 18 parts -29 parts of tapioca, 16 parts -24 parts of bean straw powder end and 7 parts -15 parts of bone meal.
2. it is according to claim 1 it is a kind of promote longan grafting wound healing consolidant, it is characterised in that the celestial being
People's palm extracts liquid and preparation method thereof：By fresh cactus deburring, smash to pieces, the percentage by volume for being subsequently adding equal quality part is
Removal filter residue is filtered after the ethyl acetate solution refluxing extraction 2h-3h of 40% (v/v) -50% (v/v) and obtains filtrate, filtrate is entered
Row vacuum distillation removes ethyl acetate, is then concentrated, and is concentrated to 1/4 stopping that concentrate is stoste volume and is concentrated to give
Cactus extraction.
3. it is according to claim 1 it is a kind of promote longan grafting wound healing consolidant, it is characterised in that the pellet
Ginseng extracts liquid and preparation method thereof：By moisture content for the red sage root of 5%-10% is cut into slices, 3-5 times of mass parts ethanol volume hundred is subsequently adding
Point content after 30d-35d, Danshen Tablets is fallen together with yellow rice wine to be soaked in the yellow rice wine of 60% (v/v) -70% (v/v)
Enter in marmite, infusion is carried out with slow fire after boiling, cooled down after 2h-3h, filter removal filter residue, filtrate is proceeded into concentration, directly
To concentrate for 1/3 stopping of stoste volume is concentrated to give red sage root extract.
4. it is according to claim 1 it is a kind of promote longan grafting wound healing consolidant, it is characterised in that it is described peppery
Knotweed grass extracts liquid and preparation method thereof：The fresh whole strain of flaccid knotweed herb is ground, is subsequently adding after the water of 2-3 times of mass parts boils
Turning slow fire carries out infusion, and flaccid knotweed herb extract solution is filtrated to get after infusion 3h-4h.
5. it is according to claim 1 it is a kind of promote longan grafting wound healing consolidant, it is characterised in that the spirit
Vanilla extracts liquid and preparation method thereof：Fresh Lysimachia foenum-graecum blade is won, the percentage by volume that 2-4 times of mass parts are added after shredding is
Removal filter residue is filtered after the butanol solution refluxing extraction 2h-3h of 20% (v/v) -30% (v/v) and obtains filtrate, filtrate is subtracted
Pressure is distilled off butanol, is then concentrated, and is concentrated to concentrate and is carried for 1/5 stopping of stoste volume is concentrated to give Lysimachia foenum-graecum
Take liquid.
6. it is according to claim 1 it is a kind of promote longan grafting wound healing consolidant, it is characterised in that the dragon
Courage grass extracts liquid and preparation method thereof：By moisture content for the felwort blade of 5%-10% is shredded, 3-5 times of mass parts are subsequently adding
Percentage by volume obtains filtrate to filter removal filter residue after the acetone soln refluxing extraction 1h-2h of 10% (v/v) -20% (v/v),
Filtrate is carried out into vacuum distillation and removes acetone, be then concentrated to 1/2 stopping that concentrate is stoste volume and be concentrated to give rough gentian
Careless extract solution.
7. it is according to claim 1 it is a kind of promote longan grafting wound healing consolidant, it is characterised in that the spirit
Sesame extracts liquid and preparation method thereof：Whole strain is put into the 5-6 times of volume basis of mass parts after moisture content is cleaned for the ganoderma lucidum of 2%-5%
Number pours into back ganoderma lucidum to soak 30d-40d then in the ethanol solution of 70% (v/v) -80% (v/v) together with ethanol solution
After carrying out refluxing extraction 1h-2h in stream tank, then filtered, filtrate is carried out into vacuum distillation removes ethanol, then extraction raffinate is entered
Row concentration, until concentrate for 1/5 stopping of stoste volume is concentrated to give lucidum extracting liquid.
8. according to claim 1-7 any one promotion longan grafting wound healing consolidant preparation method, its
It is characterised by, methods described comprises the following steps：
(1) cactus extraction, red sage root extract, flaccid knotweed herb extract solution, Lysimachia foenum-graecum extract solution, rough gentian are weighed by the weight portion
Careless extract solution and lucidum extracting liquid, then carry out being mixed to get liquid reparation constituents mixt；
(2) carry out being mixed to get solid mixing after weighing celestial analysis for soybean powder, tapioca, bean straw powder end and bone meal by the weight portion
Thing, is then processed into the powder that granularity is 200-300 mesh by solid mixture, obtains solid and repairs constituents mixt；
(3) liquid of step (1) is repaired into constituents mixt and repairs constituents mixt by the weight ratio with the solid of step (2)
After uniform mixing, the vaseline of liquid is added, be adjusted to paste and obtain consolidant.
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| CN201710052942.8A CN106927944A (en) | 2017-01-22 | 2017-01-22 | It is a kind of to promote consolidant of longan grafting wound healing and preparation method thereof |
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| CN201710052942.8A CN106927944A (en) | 2017-01-22 | 2017-01-22 | It is a kind of to promote consolidant of longan grafting wound healing and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107950602A (en) * | 2017-12-14 | 2018-04-24 | 柳州市象行教育科技有限责任公司 | A kind of special consolidant of callus and preparation method thereof |
| CN111713283A (en) * | 2020-06-30 | 2020-09-29 | 广西壮族自治区农业科学院 | Rootstock replacing method without influencing grape harvest |
| CN114391413A (en) * | 2022-01-27 | 2022-04-26 | 保亭智农农业发展有限责任公司 | Rapid cultivation method of durian seedlings |
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| CN102336610A (en) * | 2011-09-01 | 2012-02-01 | 安徽师范大学 | Chinese medicine composition for promoting plant wound heeling |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950602A (en) * | 2017-12-14 | 2018-04-24 | 柳州市象行教育科技有限责任公司 | A kind of special consolidant of callus and preparation method thereof |
| CN111713283A (en) * | 2020-06-30 | 2020-09-29 | 广西壮族自治区农业科学院 | Rootstock replacing method without influencing grape harvest |
| CN114391413A (en) * | 2022-01-27 | 2022-04-26 | 保亭智农农业发展有限责任公司 | Rapid cultivation method of durian seedlings |
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Application publication date: 20170707 |