CN108260608A - A kind of plant source growth regulator and its application in promoting crop growth long side - Google Patents
A kind of plant source growth regulator and its application in promoting crop growth long side Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/30—Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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Abstract
The invention discloses a kind of plant source growth regulator and its applications in promoting crop growth long side.The plant source growth regulator of the present invention includes 0.5 1 times of Chinese medicine composition, the cosolvent of 23 times of amounts of Chinese medicine composition gross mass and Chinese medicine composition gross mass emulsifiers measured；The Chinese medicine composition according to the mass fraction, including 20 35 mass parts of tea seed cake extract, 10 20 mass parts of yam extract, 10 20 mass parts of Semen Litchi extract, 10 15 mass parts of Rhizoma Smilacis Chinensis extract, 5 10 mass parts of Radix Flemingiae Philippinensis extract, 8 20 mass parts of Ginger P.E, 15 mass parts of 5 10 mass parts of radix-polygoni multiflori extract, 15 mass parts of shrubby sophora extract and purslane extract.The plant source growth regulator of the present invention can effectively avoid using single chemical constituent, the problem of forming drug resistance directly uses growth hormone, the problem of causing crop quality poor, with safe and non-toxic, it is easily absorbed and utilized, noresidue pollution is environmentally protective, the characteristics of promoting the growth of crops, improving crop yield and quality.
Description
Technical field：
The invention belongs to growth regulator fields, and in particular to a kind of plant source growth regulator and its long in promoting crop growth
The application of aspect.
Background technology：
Secondary metabolites be plant under adverse environmental factor, in order to response environment stress or pest and disease damage etc. threaten
A kind of chemical substance of generation, such substance for enhance plant resistance, prevent pest and disease damage invasion etc. play it is huge
Effect.Secondary metabolites include volatile oil, terpene, Phenylpropanoid Glycosides, steroidal, flavones, alkaloid and quinones etc., have growth
The effects that adjusting, expelling parasite, desinsection and raising immunity.The mankind have found certain plants during search of food and drug
Position or fruit are edible, have the function of full abdomen, it is often more important that find that it can be used for treating certain diseases, Huo Zheke
So that human body is strong, and it is full of vitality, delay too fast aging of organ etc..The discovery of these effects makes the mankind more pay attention to plant
The research of secondary metabolite illustrates the secondary generation of plant by the investigative techniques such as chemistry, biology and pharmacology and method
It thanks to product component, studies its medical value and the mechanism of action, actively make to improve the quality of life of the mankind and ensureing that health plays
With.
When scientist actively has found new plant origin and new active function, to explore new Plant Secondary Materials generation
It thanks to product and generates these chemical substances when preventing the difficult and complicated cases currently occurred, but ignoring plant and plant is developed in itself
With the effect of defence.By a large amount of theoretical research and activity experiment, research finds the secondary metabolite of most plants all
Containing flavones, phenols, triterpene and steroid chemical composition, such substance is for promoting the development of plant and enhancing the degeneration-resistant of plant
Property particularly significant, other composition of alkaloids and bitter principle constituents, have Antifeedant Effects, prevent shadow of the plant by insects
Ring, and quinones substance can effective gathering trace metallic element, it is significant for growing up strong and sturdy for crop.
Based on this principle, research is thought, the mankind are obtaining secondary metabolites from plant, while enhancing is healthy, also may be used
Think the secondary metabolite of plant addition plant source, crop is effectively adjusted by artificial addition secondary metabolites
Growth improves the resistance of crop, improves yield and the ability of disease and insect resistance.And the chemical substance of these plant sources is source
In crop in itself, it is easily absorbed by crops, noresidue, to the mankind more without toxic side effect, existing chemical drugs can be substituted completely
The effect of object, and can be the only way of Development of Organic Agriculture and green agriculture with environmental protection, it complies fully with nuisanceless new
The research direction of pesticide.
Invention content：
The object of the present invention is to provide a kind of plants that can enhance crop disease-resistant ability while plant growth is promoted
Source growth regulator, the plant source growth regulator can effectively avoid, using single chemical constituent, forming asking for drug resistance
Topic or directly using growth hormone the problem of causing crop quality poor, has safe and non-toxic, is easily absorbed and utilized, noresidue is dirty
Dye, it is environmentally protective, the characteristics of promoting the growth of crops, improve crop yield and quality.
China's Chinese herbal medicine resource is quite abundant, therefore this patent invents a kind of plant source growth just using Chinese herbal medicine as raw material
Conditioning agent.The Chinese herbal medicine of this patent design is as follows：
Tea seed cake：Tea seed cake is also tea bran, tea seed cake, is a blob of slag of the tea seed after oil expression, and active ingredient is Tea Saponin, tea
Tea Saponin containing 12-18% in withered.Tea Saponin has pest good stomach toxicity and action of contace poison, can be extensively in vegetables, water
Rice fruit tree, tealeaves, is used in flowers and other crops, prevention snail, river snail, leech, planthopper, rice leafhopper, the first-class evil of sweet potato baby elephant
Worm.The protein content of tea seed cake is higher, is a kind of efficient organic fertilizer, is widely used in crops and fruit tree plantation, effect pole
It is good.Containing the ingredients such as Tea Saponin, Residual oil, protein, starch and carbohydrate, cellulose in tea seed cake, have to the growth of plant and promote
Effect.
Chinese yam：Rhizoma dioscoreae are the stem tuber of Dioscoreaceae plant Chinese yam, belong to perennial voluble herb plant.It is former
Name Chinese yam, also known as Chinese yam rhizome, RHIIZOMA DIOSCOREAE from Henan of China, Chinese yam, Chinese yam, beautiful taro etc..Chinese yam for the most popular Traditional health care food in China it
One, other than a small number of torrid areas, almost there are cultivation in various regions, and main product is in Henan Province, the ground such as Hunan, Jiangnan.Its sweet in flavor and neutral in nature,
Returns spleen, lung, kidney channel, primary efficacy are supplementing qi and nourishing yin, tonifying spleen lung kidney.The content of K, P, Na are very high in Chinese yam, have apparent rich
Collection acts on.The main starch-containing, protein of Chinese yam stem tuber, Free Amino Acids and polysaccharide, allantoin, amylase, choline, wheat
The various actives substance such as angle sterol, campesterol, cupreol, polyphenol oxidase.Chinese yam main component Chinese yam polysaccharide sprays work
It after object, can digest as oligosaccharide molecular segment, and smoothly participate in the ability of crop into the biosynthesis of crop and metabolic system
Conversion, promotes the growth of crop and the accumulation of nutriment.
Chinaroot greenbrier：Smilax china L. are Liliaceae chinaroot greenbrier platymiscium, perennial liana fallen leaves climber.Root-like stock
It is thick thick, it is hard, it is irregular bulk.It is born in the hayashishita below 2000 meters of height above sea level, in shrubbery, on roadside, river valley or hillside.Point
It is distributed in drought various regions, production Southwest China, Central-South and East China, Korea, Japan, Burma, Vietnam, Thailand, Philippine etc.
Ground is also distributed.There is dispelling wind and eliminating dampness, dissipate the stasis of blood, promoting blood circulation and detoxication, relieving convulsion ceases wind, anticancer and other effects；It is clinically used for treatment rheumatic waist
Skelagia, child convulsion, enteritis, boil, scrofula, cancer etc..In chinaroot greenbrier in addition to containing nutritional ingredients such as carbohydrate, fat, protein,
Also containing bioactive ingredients such as steroid saponin, flavonoids, Anthraquinones, phenols, Coumarins, alkaloids.The spy of chinaroot greenbrier
Sign property ingredient, smilacin have the structure of similar brassinosteroid, therefore show plant growth regulator effect, low dense
Under the action of degree, plant growth can be promoted, enhance the resistance of crop, improve yield.
Semen litchi：Dry mature seed for sapindaceous plant lichee Litchi chinensis Sonn..Lichee category
Aiphyllium is distributed in the ground such as Fujian, Guangdong, Guangxi.Semen litchi is warm-natured, has qi-regulating, dispelling cold, analgesic, hypoglycemic, reducing blood lipid
The effect of, available for gastral cavilty long pain, stagnation of QI due to depression of the liver, hernia pain, women qi stagnation blood stasis abdominal pain, testiclar gall etc..In semen litchi
Contain the bioactive ingredients such as organic acid, fatty acid, amino acids, saponins, flavonoids, carbohydrate.Semen litchi main component
Amino acid is natural origin amino acid, can supplement the needs with the maturity period to amino acid as growth, improves the quality of product.
Waras：Flemingia leguminosae are pulse family Moghania plant, and alias Flemingia philippinensis is entered with root
Medicine is grown in slight slope thick grass, is distributed in the ground such as Fujian, Taiwan, Hubei, Hunan, Guangxi, Hainan, Guizhou, Yunnan, is had only
The effect of cough eliminating the phlegm, qi-restoratives labor, strengthens the bone at removing damp-heat, wind-damp dispelling, strong waist and knee.The isolated compound from waras
Have flavonoids, Anthraquinones, Coumarins etc. he.The prenyl isoflavones being wherein rich in have it is anti-inflammatory, improve immunity, be anti-
The multiple biological activities such as virus.Prenyl isoflavones contained by waras have strong resistant effect, common to plant
Fungal disease have inhibiting effect.
Ginger：Ginger is the new fresh quench of Zingiber herbaceos perennial ginger (Zingiber officinale Roscoe)
Stem, alias have race, hundred peppery clouds, hook dress refer to, because ground is pungent, hot and cold boy, fresh ginger, honey toast ginger.Its acrid flavour is warm-natured, is in the world
One of important condiment product has plantation in Asia, Africa and Latin America.Ginger has relieving exterior syndrome and dispelling cold, warming middle energizer to arrest vomiting, resolving sputum
The effect of cough-relieving, cures mainly anemofrigid cold, gastrofrigid vomiting, cold productive cough and coughs.Modern research shows that ginger has anti-oxidant, the anti-inflammatory, heart
Vascular protection, hypoglycemic, reduction cholesterol and antineoplastic action.Main active and characteristic taste compound in ginger
For gingerol, gingerol can be divided into the different types such as gingerol, salad oil, secondary zingerone；Ginger also contains abundant nutriment,
Carbohydrate, fat, protein, cellulose, carrotene, vitamin C etc..Gingerol contained by ginger is natural pest-resistant ingredient, is led to
The stomach effect of touching is crossed, insect pest is resisted and food is invaded to plant.
The fleece-flower root：Polygonum muliflorum derive from the dried root of polygonum multiflorum thunb.The Song dynasty《Solar corona
Book on Chinese herbal medicine》Record " its medicine book on Chinese herbal medicine is unknown, because the fleece-flower root is shown in that rattan night hands over, just searches for food active, because to adopt artificial name ear, " traditional Chinese medicine is recognized
It is warm-natured for, fleece-flower root bitter, puckery.Return liver, the heart, kidney channel.Life removing toxicity for eliminating carbuncles, relaxes bowel, for filling liver kidney, benefiting essence-blood, crow
Beard and hair, strengthening the bones and muscles.Modern research shows that the fleece-flower root mainly contains the ingredients such as anthraquinone, Stibene-glucoside, phosphatide, have by blood fat,
Delay atherosclerosis, anti-aging, improve the effects that immunity, intelligence development.In the fleece-flower root containing abundant Mg, Fe, Mn, Zn and
The trace elements such as Al.The anthraquinone component of the fleece-flower root such as Physcion etc. has toxic action to insect, is natural desinsection
Agent.
Kuh-seng：Sophora flavescens are the dry roots of pulse family Sophora kuh-seng, are distributed in all parts of the country.The change of kuh-seng
Study point research shows that, kuh-seng contains two major class chemical compositions：Alkaloid and flavone compound and kuh-seng pharmacological action
Main component.Kuh-seng alkaloid is quinolizidine kind mostly, and only a few is Bispiperidines.Kuh-seng have anti parasitic, antibacterial,
It the effects that antiviral, antiallergy, is widely used in clinic.Matrine is one of main component of biological pesticide, is had to insect
There is stomach poison function, be pure natural insecticide.
Purslane：Portulaca oleracea L. be the annual meat herbaceous plant of Portulacaceae Portulaca also known as
Five-element's grass, locust dish, longevity greens/mustard green, horse step on dish etc., and Chinese north and south various regions are produced, and it is moist fertile to be born in vegetable garden, farmland and mountain area
Place abounds in Sichuan, spreads all over the country.Not only can be used as medicine, but also edible.The effect of with clearing heat and detoxicating, cooling blood and hemostasis.For controlling
It treats under hot dysentery purulence blood, band, erysipelas etc..In recent years pharmacological research shows：Purslane also has reducing blood lipid, hypoglycemic, anti-athero- dynamic
Arteries and veins hardening and other effects.A plurality of types of chemical compositions such as alkaloid, steroidal, terpene are mainly contained in purslane.Purslane is extracted
Object has the apparent bioactivity for promoting plant drought effect, can effectively improve the drought tolerance and drought-resistant ability of plant.
The purpose of the present invention is achieved through the following technical solutions：
The plant source growth regulator of the present invention, the hydrotropy measured including Chinese medicine composition, 2-3 times of Chinese medicine composition gross mass
The emulsifier that 0.5-1 times of agent and Chinese medicine composition gross mass are measured；
The Chinese medicine composition according to the mass fraction, including tea seed cake extract 20-35 mass parts, yam extract 10-
20 mass parts, Semen Litchi extract 10-20 mass parts, Rhizoma Smilacis Chinensis extract 10-15 mass parts, Radix Flemingiae Philippinensis extract 5-10 mass
Part, Ginger P.E 8-20 mass parts, radix-polygoni multiflori extract 5-10 mass parts, shrubby sophora extract 1-5 mass parts and purslane carry
Take object 1-5 mass parts.
The cosolvent is the ethanol water of volume fraction 95%.
The emulsifier is Tween 80, polysorbate60, polyoxyethylene or the single hard fatty acids of glycerine 400.
The plant source growth regulator is prepared by the following method, according to the mass fraction, by tea seed cake extract
20-35 mass parts, yam extract 10-20 mass parts, Semen Litchi extract 10-20 mass parts, Rhizoma Smilacis Chinensis extract 10-15 mass
Part, Radix Flemingiae Philippinensis extract 5-10 mass parts, Ginger P.E 8-20 mass parts, radix-polygoni multiflori extract 5-10 mass parts, kuh-seng carry
Object 1-5 mass parts and purslane extract 1-5 mass parts is taken to be uniformly mixed to obtain Chinese medicine composition, then in Chinese medicine composition add
Enter 0.5-1 times of the ethanol water for the volume fraction 95% that 2-3 times of Chinese medicine composition gross mass is measured and Chinese medicine composition gross mass
The emulsifier of amount.
The tea seed cake extract, yam extract, Semen Litchi extract are obtained using hot water boiling method：Specifically by Chinese medicine
Raw material crushes after drying in the shade, and is added in hot water extractor, extraction 3.5-4.5h is boiled with hot water, by residue reusable heat water after filtering
Extraction 2.5-3.5h is boiled, is filtered, merging filtrate, after concentrating filter liquor, then it is freeze-dried, you can obtain extract.
The addition of each water is 8-10 times of Chinese medicine material gross weight during the extraction.
The Rhizoma Smilacis Chinensis extract, Radix Flemingiae Philippinensis extract, Ginger P.E, radix-polygoni multiflori extract, shrubby sophora extract and dent
Amaranth extract is obtained using solvent extraction method：It crushes after specifically Chinese medicine material is dried in the shade, is eaten with volume fraction 60-95%
Alcohol at normal temperature extracts 16-48h, and residue is extracted 12-36h, mistake with volume fraction 60-95% ethanol waters room temperature again after filtering
Filter, merging filtrate, after concentrating filter liquor, then it is freeze-dried, you can obtain extract.
The addition of each solvent is 8-10 times of Chinese medicine material gross weight during the solvent extraction.
It is preferred that the Rhizoma Smilacis Chinensis extract, radix-polygoni multiflori extract are extracted with volume fraction 60%-70% ethanol waters；
Ginger P.E, shrubby sophora extract are extracted with volume fraction 70%-80% ethanol waters；Radix Flemingiae Philippinensis extract, purslane carry
Object volume fraction 80%-95% ethanol waters is taken to extract.
The freeze-drying, process conditions are：Precooling temperature is -20 DEG C~-50 DEG C, the precooling time for 15~
30h, then vacuumize drying under the decompression of 4Pa~107Pa so that product temperature during dry completion is less than 30 DEG C.It obtains
Extract powder moisture≤5%.
Another object of the present invention is just to provide the plant source growth regulator in terms of crop growth is promoted
Using.The plant source growth regulator has multi-functional and broad spectrum activity, is particularly suitable for modern organic agriculture, forestry, animal husbandry,
It is not influenced by natural environment and weather conditions, type, the kind of unlimited fabrication, use scope is extensive.The plant source is grown
Conditioning agent can effectively adjust the growth of crop, improve the dry-matter accumulation of crop, enhance the resistance of crop, enhance farming
The ability that object is withstood natural calamities, the effective yield for improving crop, and there is prevention to make various plants fungal disease, insect pest
With.
The effect innovative point of the present invention：
The plant source growth regulator of the present invention is the active secondary metabolite extracted in traditional Chinese medicine, freeze-dried
It is formed afterwards with cosolvent and emulsifier mixed preparing.The simple production process of the present invention is ripe, and operating process is short, edible second used
Alcohol can recycle again, and production cost is low, be easy to industrialization production.
Plant used in the present invention is traditional Chinese medicine, there is cultivation throughout the country, resourceful, be easy to get and into
This is relatively low, and Chinese medicine is renewable resource, can realize Substantial evaluation.Chinese herbal medicine used is nontoxic, uses peace
Entirely.
The plant source growth regulator of the present invention is derived from special biology of the Secondary metabolites in plant
Function and design invention.The plant source growth regulator of the present invention is rich in amino acid, polysaccharide, flavones, sterol, anthraquinone, biology
The ingredients such as alkali and trace element realize Multiple components efficient combination, synergistic, and function and effect protrude notable.And biology
Alkali components have the function of antibacterial, desinsection, in the disease resistance for promoting can to enhance while plant growth crop, effectively
The problem of avoiding using single chemical constituent, forming drug resistance.Or directly cause crop quality poor using growth hormone
Defect.
Specific embodiment：
For ease of more fully understanding the present invention, it is illustrated by following instance.These examples belong to the protection of the present invention
Range, but do not limit the scope of the invention.
Embodiment 1：The preparation method of Chinese medical extract
Tea seed cake extract, yam extract, Semen Litchi extract are obtained using hot water boiling method：By Chinese medicine material (tea seed cake,
Chinese yam or semen litchi) dry in the shade after crush, according to quality of material than 1:10 are added in hot water extractor, boil extraction with hot water
4h, after filtering by residue according still further to quality of material than 1:8 add in water, boil extraction 3h, filtering with hot water, merging filtrate will be filtered
After liquid is concentrated, then it is freeze-dried, you can obtain extract.
Rhizoma Smilacis Chinensis extract, Radix Flemingiae Philippinensis extract, Ginger P.E, radix-polygoni multiflori extract, shrubby sophora extract and purslane carry
Object is taken to be obtained using solvent extraction method：It is crushed after Chinese medicine material (chinaroot greenbrier, waras, ginger or the fleece-flower root) is dried in the shade, according to
Quality of material is than 1:10 add in volume fraction 60-95% edible ethanols in extractor, and room temperature is extracted for 24 hours, by residue after filtering
Again with quality of material than 1:8 volume fraction 60-95% alcohol at normal temperature extraction 18h, filtering, merging filtrate, by concentrating filter liquor
Afterwards, it is then freeze-dried, you can to obtain extract.
The Rhizoma Smilacis Chinensis extract, radix-polygoni multiflori extract are extracted with 65% ethanol water of volume fraction；Ginger P.E,
Shrubby sophora extract is extracted with 75% ethanol water of volume fraction；Radix Flemingiae Philippinensis extract, purslane extract volume fraction
85% ethanol water extracts.
The process conditions of the freeze-drying are：Precooling temperature is -20 DEG C~-50 DEG C, the precooling time for 15~
30h carries out vacuumizing drying under the decompression of 4Pa~107Pa so that product temperature during dry completion is 30 DEG C hereinafter, obtaining
Extract powder moisture≤5% arrived.
Thus tea seed cake extract, yam extract, Semen Litchi extract, Rhizoma Smilacis Chinensis extract, Radix Flemingiae Philippinensis extract, life are obtained
Ginger extract, radix-polygoni multiflori extract, shrubby sophora extract and purslane extract.
Embodiment 2：The preparation method of plant source growth regulator
Plant source growth regulator A：It is measured by scientific experiment proportioning and active ingredient interaction effect, gained extraction
Object mixed proportion is：Tea seed cake extract 30g, yam extract 15g, Semen Litchi extract 15g, Rhizoma Smilacis Chinensis extract 10g, waras
Extract 8g, Ginger P.E 8g, radix-polygoni multiflori extract 8g, shrubby sophora extract 3g, purslane extract 3g are consolidated after mixing
Body mixture of powders.Solid powder mixture in mass ratio 1：It is water-soluble that 2.5 ratio is added to 95% edible ethanol of volume fraction
In liquid, emulsifier tween -80 for 0.8 times of amount of solid powder mixture quality are then added, are sufficiently stirred to get plant source
Growth regulator A.In use, being diluted with water 300-500 times to get to the aqueous emulsion of plant source growth regulator A, directly act on
In crops.
Plant source growth regulator B：It is measured by scientific experiment proportioning and active ingredient interaction effect, gained extraction
Object mixed proportion is：Tea seed cake extract 25g, yam extract 10g, Semen Litchi extract 10g, Rhizoma Smilacis Chinensis extract 15g, waras
Extract 10g, Ginger P.E 10g, radix-polygoni multiflori extract 5g, shrubby sophora extract 5g, purslane extract 1g are obtained after mixing
Solid powder mixture.Solid powder mixture in mass ratio 1：It is water-soluble that 2 ratio is added to 95% edible ethanol of volume fraction
In liquid, emulsifier tween -60 for 1 times of amount of solid powder mixture quality are then added, is sufficiently stirred and is given birth to get plant source
Long conditioning agent B.In use, being diluted with water 300-500 times to get to the aqueous emulsion of plant source growth regulator B, directly act on
Crops.
Plant source growth regulator C：It is measured by scientific experiment proportioning and active ingredient interaction effect, gained extraction
Object mixed proportion is：Tea seed cake extract 20g, yam extract 20g, Semen Litchi extract 20g, Rhizoma Smilacis Chinensis extract 12g, waras
Extract 5g, Ginger P.E 20g, radix-polygoni multiflori extract 10g, shrubby sophora extract 1g, purslane extract 5g are obtained after mixing
Solid powder mixture.Solid powder mixture in mass ratio 1：It is water-soluble that 3 ratio is added to 95% edible ethanol of volume fraction
In liquid, emulsifier tween -60 for 0.5 times of amount of solid powder mixture quality are then added, are sufficiently stirred to get plant source
Growth regulator C.In use, being diluted with water 300-500 times to get to the aqueous emulsion of plant source growth regulator, directly act on
In crops.
Embodiment 3：Main component, content and the assay method of plant source growth regulator：
The present invention plant source growth regulator constituent contain amino acid, aliphatic acid, polysaccharide, flavones, alkaloid,
The ingredients such as anthraquinone, trace element.Plant source growth regulator main component of the invention is after measured：Organic acid 300-500mg/
L (neutralization titration measure), free amino acid 200-300mg/L (ninhydrin method measure), soluble sugar 500-800mg/L (anthracenes
Ketone method measures), general flavone 100-200mg/L (ultraviolet spectrophotometry), general anthraquinone 100-200mg/L (ultraviolet specrophotometers
Method), total alkaloid 10-20mg/L (volumetric method), micro- 0.5-1mg/L (flame atomic absorption spectrometry).
Embodiment 4：The experiment that plant source growth regulator A sprouts green cucumber seed
Green cucumber seed sprouts experiment using culture dish filter paper method.Take the culture dish of diameter 10cm, interior 2 layers of filter paper of paving, difference
With the plant source growth regulator A (CK, 0.1,0.2,0.5,1,2mg/L) of each concentration, Rhizoma Smilacis Chinensis extract (0.2,0.5mg/L),
Radix Flemingiae Philippinensis extract (0.2,0.5mg/L), Semen Litchi extract (0.2,0.5mg/L), yam extract (0.2,0.5mg/L) and
Positive control gibberellin (30ppm) moistens, and 20 cucumber are uniformly placed on filter paper, and culture dish is kept to moisten.It will culture
Ware is positioned in incubator, and set temperature is 25 DEG C, adds alignment processing liquid 5mL into culture dish per 12h, is kept in culture dish
Moistening.Per chitting piece number is recorded for 24 hours, germinating energy and germination percentage are calculated.The influence that plant source growth stimulator A sprouts green cucumber seed
It is shown in Table 1.CK is water.
The influence that 1 plant source growth stimulator A of table sprouts green cucumber seed
As it can be seen from table 1 the plant source growth regulator A of suitable concentration is promoting green cucumber seed to sprout than single extract
Originating party face is better, illustrates there is synergistic effect between each extract, is preferably used in mixed way with suitable ratio.Also may be used from table 1
Knowing, the plant source growth regulator A of various concentration shows the trend for first increasing and reducing afterwards when green cucumber seed is promoted to sprout,
The feature of rush is lowered with height.Green cucumber seed is handled in a concentration of 0.2mg/L, significantly seed can be promoted to sprout, kind
Sub- germination percentage is 95%, suitable with gibberellin effect；Reduce the sprouting impetus of seed instead when concentration is increased to 2mg/L,
Germination percentage drops to 85%, identical with control group.Illustrate that plant source growth regulator A can significantly improve seed in 0.2mg/L
Germination percentage, and be then unfavorable for seed sprouting during high concentration, need to control plant source growth regulator A in practical agricultural production
Activity use.
Embodiment 5：Influences of the plant source growth regulator A to green cucumber growth of seedling
The experiment of green cucumber seed seedling is using culture dish filter paper method.Take the culture dish of diameter 10cm, interior 2 layers of filter paper of paving, difference
With each concentration (CK, 0.1,0.2,0.5,1,2mg/L) of plant source growth regulator A, Rhizoma Smilacis Chinensis extract (0.2,0.5mg/L),
Radix Flemingiae Philippinensis extract (0.2,0.5mg/L), Semen Litchi extract (0.2,0.5mg/L), yam extract (0.2,0.5mg/L) and
Positive control gibberellin (30ppm) moistens, and 20 cucumber are uniformly placed on filter paper, and culture dish is kept to moisten.It will culture
Ware is positioned in incubator, and set temperature is 25 DEG C, adds alignment processing liquid 5mL into culture dish per 12h, is kept in culture dish
Moistening.When its seedling grows to a leaf wholeheartedly, thick its plant height, stem, leaf area, fresh weight and dry weight are measured.Plant source growth regulating
Treated that green cucumber growth of seedling situation is shown in Table 2 by agent A.CK is water.
Influences of the 2 plant source growth regulator A of table to green cucumber growth of seedling
From table 2 it can be seen that the plant source growth regulator A of suitable concentration is promoting green cucumber seedling to give birth to than single extract
Long side is better, has synergistic effect between each extract.It will also realize that from table 2, with the plant source growth regulating of various concentration
Agent A, compared with the control, green cucumber Growth of seedling are dramatically different.Aggregate analysis, seedling stem is thick, leaf area, seedling fresh weight and dry weight
Show the trend for first increasing and reducing afterwards, the green cucumber seedling of the plant source growth regulator A processing of wherein 0.2-0.5mg/L, children
Seedling growing way is best, and stem is thick, leaf area, seedling fresh weight and dry weight effect are significantly higher than other processing, suitable with gibberellin；And
For the stem length of seedling then present to be that concentration more high effect is better in plant source growth regulator A institutes test concentrations become
Gesture.Therefore it during use, grows tall as far as possible if necessary to plant, then can suitably increase concentration.
Embodiment 6：Influence of the plant source growth regulator A foliage-sprays to growth of seedling
Influence of the plant source growth regulator A foliage-sprays to growth of seedling is measured using potted plant experiment, and experiment sets 15
Processing group, respectively：CK, plant source growth regulator A (0.1,0.2,0.5,1,2mg/L), it is chinaroot greenbrier (02,0.5mg/L), very heavy
Pull out (02,0.5mg/L), semen litchi (02,0.5mg/L), Chinese yam (02,0.5mg/L), gibberellin 30ppm, each processing group 6
It repeats, soil 5kg is filled per basin, per the green cucumber seedling 2 of pot transplanting nursery about 4cm high, place it under natural environment and cultivated.
Treat that green cucumber growth of seedling to one heart stage of three leaves progress foliage-spray, measures thick its plant height, stem, the number of blade, fresh weight and does after five days
Weight.Treated that green cucumber growth of seedling situation is shown in Table 3 for plant source growth regulator A foliage-sprays.CK is water.
Influence of the 3 plant source growth regulator A foliage-sprays of table to green cucumber single plant growth of seedling
From table 3 it can be seen that the plant source growth regulator A of suitable concentration is being promoted than single extract using spraying method
It is better in terms of green cucumber growth of seedling, there is synergistic effect between each extract.It will also realize that from table 3, plant source growth regulating
The growth of seedling index of correlation that agent A is sprayed shows well, shows the trend for first increasing and reducing afterwards, wherein 0.2-0.5mg/L
Plant source growth regulator A processing green cucumber seedling effect it is the most apparent, each index be all significantly higher than control and other processing,
Plant height, stem are thick, the number of blade, root long, fresh weight, dry weight when 0.2,0.5mg/L handle respectively than control raising 25.04%,
26.22%th, 20.00%, 374.63%, 60.37%, 81.63% and 27.04%, 30.00%, 40.00%, 293.39%,
43.53%th, 40.82%, it is suitable with gibberellin.Although effect is also above control when concentration is increased to 2mg/L, effect is not
It is prominent, therefore when in use it is noted that the control of concentration.
7 plant source growth regulator A roots of embodiment spray the influence grown to green cucumber
Plant source growth regulator A roots, which are sprayed, measures the influence that green cucumber is grown using potted plant experiment, and experiment is set at 2
Reason group (CK, plant source growth regulator A0.3mg/L), each 6 repetitions of processing group fill soil 5kg per basin, per each concentration of basin
Plant source growth regulator A 100mL are fitted into after each concentration plant source growth regulator A of 100mL are mixed with soil in basin.Often
The green cucumber seedling of pot transplanting nursery about 4cm high 2, places it under natural environment and is cultivated.Fertilising in every 15 days later is primary,
It uproots plants after their edible portions have been harvested after 2 months and measures its ground fresh weight, ground dry weight, underground fresh weight, underground dry weight, melon fresh weight, melon dry weight, fruits aqueous
Rate, knot melon number, yield.Plant source growth regulator A roots spray that treated, and green cucumber growing state is shown in Table 4.CK is water.
4 plant source growth regulator A roots of table spray the influence grown to green cucumber
From table 4, it can be seen that the green cucumber growth index of correlation that plant source growth regulator A roots spray shows well,
Each index is all significantly higher than control, and the fresh weight and dry weight of the root of plant source growth regulator A processing are that control group improves respectively
9.73%th, 50.77%, illustrate that plant source growth regulator A can promote the growth of green cucumber root system；Plant source growth regulator A
The photosynthetic rate of processing group improves 25.32% than control group, while observes plant source growth regulator A processing groups blade more
Greatly, color is greener, illustrates that plant source growth regulator A can improve the content of green cucumber chlorophyll, improves the photosynthetic rate of green cucumber.
Plant source growth regulator A can promote the comprehensive function of root and photosynthetic rate the accumulation of green cucumber dry matter, improve green cucumber
Yield (improves 53.97%).
7 organic vegetable simulation experiments of embodiment
Vegetables green cucumber, tomato, capsicum, the fresh kidney beans that this experiment mainly selects family's daily consumption more are research object,
Each vegetables sets 2 processing (CK, plant source growth regulator A), each handles 1 mu of ground, in the critical stage of growth, including battalion
Health length, bud differentiation, fruit development and fruit maturation stage spray processing group spray on plants source growth regulator A, CK groups
Moisture.Its disease-resistant insect resistance capacity is observed during vegetable growth, the weight of each processing group vegetables is weighed when fruiting period receives fruit every time
The sum of amount, total weight of vegetables are the yield of vegetables.Plant source growth regulator A is shown in the effect of increasing production of each organic vegetable
Table 5.
5 plant source growth regulator A of table is to the effect of increasing production of organic vegetable
As can be seen from Table 5, plant source growth regulator A of the invention is to common are machine vegetables tomato, fresh kidney beans, eggplant
Son, the growth of capsicum and green cucumber and output increased have obvious action, particularly with the output increased of tomato and green cucumber extremely
Significantly, it has been respectively increased 26.98%, 23.18%；It is also improved to some extent for the yield of fresh kidney beans and capsicum, carries respectively
It is high by 14.20%, 14.09%；It is for the increase rate of eggplant yield smaller, but also make the output increased 8.4% of eggplant.Together
When and contrast ratio, can effectively improve the anti-adversity ability of plant, there is certain defense reaction to disease.Therefore plant source growth
Conditioning agent A can be used for the popularization of agricultural production.
8 ginger of embodiment, kuh-seng, tea seed cake and radix-polygoni multiflori extract are to the toxicity test of bean aphid
Using Airbrush spray-on processes, cabbage leaves with card punch are broken into the leaf butterfly of diameter 1cm, each extract is diluted
Into the solution of 10mg/L, per the spraying of leaf dish positive and negative, spouting liquid 0.5ml, 10 test worms of often processing access, are often handled after drying in the shade
It is repeated 3 times.24 degrees Celsius, relative humidity 60%-70% are put into after processing, survival worm is investigated in the indoor culture of no light afterwards for 24 hours
Number calculates the death rate, and carries out Virulent Analysis.Ginger, kuh-seng, tea seed cake and radix-polygoni multiflori extract are to the toxicity test of bean aphid
It is shown in Table 6.
6 ginger of table, kuh-seng, tea seed cake and radix-polygoni multiflori extract are to the toxicity test of bean aphid
Table 6 tests ginger, kuh-seng, tea seed cake and radix-polygoni multiflori extract and the toxicity of bean aphid is tried under concentration 10mg/L
It tests, the experimental results showed that, after ginger, kuh-seng, tea seed cake and radix-polygoni multiflori extract handle bean aphid for 24 hours under 10mg/L, to silkworm
Bean aphid worm have must toxic action, wherein shrubby sophora extract murders rate highest, the death of bean aphid to bean aphid
Rate is 23.33%.
8 plant source growth regulator A of embodiment is to the toxicity test of bean aphid
Using Airbrush spray-on processes, cabbage leaves with card punch are broken into the leaf butterfly of diameter 1cm, reagent agent is diluted
Into a series of concentration, per the spraying of leaf dish positive and negative, spouting liquid 0.5ml, 10 test worms of often processing access, often handle weight after drying in the shade
It is 3 times multiple.24 degrees Celsius, relative humidity 60%-70% are put into after processing, the indoor culture of no light, investigation in every 12 hours is survived
Borer population calculates the death rate, and carry out Virulent Analysis after 48h.Plant source growth regulator A is shown in Table the toxicity test of bean aphid
7-9。
7 plant source growth regulator A of table is to the toxicity test one (1mg/L) of bean aphid
8 plant source growth regulator A of table is to the toxicity test two (5mg/L) of bean aphid
9 plant source growth regulator A of table is to the toxicity test three (10mg/L) of bean aphid
Table 6-8 tests A pairs of the plant source growth regulator of the present invention from tri- kinds of activities of 1mg/L, 5mg/L and 10mg/L
The toxicity of bean aphid, the experimental results showed that, when plant source growth regulator A of the invention shows the toxicity of bean aphid
Between dependence and concentration dependent, with concentration increase and action time increase, the toxicity of bean aphid is also increased,
The death rate gradually increases, and in 48h, the death rate of bean aphid is respectively under tri- kinds of activities of 1mg/L, 5mg/L and 10mg/L
23.33%th, 33.33% and 36.67%.Illustrate that plant source growth regulator A has bean aphid certain toxic action.
It is found through experiment that plant source growth regulator B and C are also similar with plant source growth regulator A such as embodiment 4-8
As effect.
Claims (10)
1. a kind of plant source growth regulator, which is characterized in that measured including Chinese medicine composition, 2-3 times of Chinese medicine composition gross mass
Cosolvent and the emulsifier measured of 0.5-1 times of Chinese medicine composition gross mass；
The Chinese medicine composition according to the mass fraction, including tea seed cake extract 20-35 mass parts, yam extract 10-20 matter
Measure part, Semen Litchi extract 10-20 mass parts, Rhizoma Smilacis Chinensis extract 10-15 mass parts, Radix Flemingiae Philippinensis extract 5-10 mass parts, life
Ginger extract 8-20 mass parts, radix-polygoni multiflori extract 5-10 mass parts, shrubby sophora extract 1-5 mass parts and purslane extract 1-
5 mass parts.
2. plant source growth regulator according to claim 1, which is characterized in that the cosolvent is volume fraction
95% ethanol water.
3. plant source growth regulator according to claim 1, which is characterized in that the emulsifier is Tween 80, spits
Temperature 60, polyoxyethylene or the single hard fatty acids of glycerine 400.
4. plant source growth regulator according to claim 1, which is characterized in that the plant source growth regulator is
It being prepared by the following method, Chinese medical extract mass fraction is uniformly mixed to obtain Chinese medicine composition according to claim 1,
The ethanol water and Chinese medicine group of Chinese medicine composition 2-3 times of volume fraction 95% measured of gross mass are added in Chinese medicine composition again
It closes object 0.5-1 times of emulsifier measured of gross mass, is uniformly mixed to obtain the final product.
5. Chinese medicine composition according to claim 1, which is characterized in that the tea seed cake extract, yam extract, lichee
Nuclear extract is obtained using hot water boiling method, is crushed, is added in hot water extractor after specifically Chinese medicine material is dried in the shade, with heat
Boiling boiling extraction 3.5-4.5h, boils extraction 2.5-3.5h, filtering, merging filtrate, by filtrate after filtering by the boiling of residue reusable heat
After concentrated, then it is freeze-dried, you can obtain extract.
6. Chinese medicine composition according to claim 5, it is characterised in that：The addition of each water is Chinese medicine during the extraction
8-10 times of raw material gross weight.
7. Chinese medicine composition according to claim 1, which is characterized in that the Rhizoma Smilacis Chinensis extract, Radix Flemingiae Philippinensis extract, life
Ginger extract, radix-polygoni multiflori extract, shrubby sophora extract and purslane extract are obtained using solvent extraction method, specifically by
Medicine raw material crushes after drying in the shade, and extracts 16-48h with volume fraction 60-95% ethanol waters room temperature, residue is used body again after filtering
Fraction 60-95% ethanol waters room temperature extracts 12-36h, filters, merging filtrate, after concentrating filter liquor, then it is chilled dry
It is dry, you can to obtain extract.
8. Chinese medicine composition according to claim 7, which is characterized in that the addition of each solvent during the solvent extraction
It is 8-10 times of Chinese medicine material gross weight, the Rhizoma Smilacis Chinensis extract, radix-polygoni multiflori extract volume fraction 60%-70% ethanol waters
Solution extracts；Ginger P.E, shrubby sophora extract are extracted with volume fraction 70%-80% ethanol waters；Radix Flemingiae Philippinensis extract,
Purslane extract is extracted with volume fraction 80%-95% ethanol waters.
9. according to the Chinese medicine composition described in claim 5 and 7, it is characterised in that：The freeze-drying, process conditions
For：Precooling temperature is -20 DEG C~-50 DEG C, and the precooling time is 15~30h, is freezed under the decompression of 4Pa~107Pa
It is dry.
10. application of the Chinese medicine composition of any one of claim 1-9 in terms of crop growth is promoted.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109430305A (en) * | 2018-11-29 | 2019-03-08 | 淳安县蚕桑管理总站 | It is a kind of for the insecticide and its preparation process of diaphania and application |
| CN109938055A (en) * | 2019-04-10 | 2019-06-28 | 江西鑫邦科技有限责任公司 | It is a kind of for preventing and treating the composition pesticide of rice sheath blight disease |
| CN113545266A (en) * | 2021-07-23 | 2021-10-26 | 扬州大学 | Method for promoting growth of taro and expansion of corm |
| CN113951285A (en) * | 2021-10-13 | 2022-01-21 | 湖南省农业环境生态研究所 | Plant growth regulator and preparation method thereof |
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| CN1817140A (en) * | 2006-02-05 | 2006-08-16 | 南京信息工程大学 | Plant-source growth regulator and use for improving crop growth |
| CN101258861A (en) * | 2008-04-24 | 2008-09-10 | 西南大学 | Use of southernwood extract as plant growth regulator and adverse-resistant agent |
| CN105519612A (en) * | 2015-12-16 | 2016-04-27 | 罗永城 | Botanical insecticide for fruit trees, preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1817140A (en) * | 2006-02-05 | 2006-08-16 | 南京信息工程大学 | Plant-source growth regulator and use for improving crop growth |
| CN101258861A (en) * | 2008-04-24 | 2008-09-10 | 西南大学 | Use of southernwood extract as plant growth regulator and adverse-resistant agent |
| CN105519612A (en) * | 2015-12-16 | 2016-04-27 | 罗永城 | Botanical insecticide for fruit trees, preparation method and application thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109430305A (en) * | 2018-11-29 | 2019-03-08 | 淳安县蚕桑管理总站 | It is a kind of for the insecticide and its preparation process of diaphania and application |
| CN109938055A (en) * | 2019-04-10 | 2019-06-28 | 江西鑫邦科技有限责任公司 | It is a kind of for preventing and treating the composition pesticide of rice sheath blight disease |
| CN113545266A (en) * | 2021-07-23 | 2021-10-26 | 扬州大学 | Method for promoting growth of taro and expansion of corm |
| CN113951285A (en) * | 2021-10-13 | 2022-01-21 | 湖南省农业环境生态研究所 | Plant growth regulator and preparation method thereof |
| CN113951285B (en) * | 2021-10-13 | 2023-05-30 | 湖南省农业环境生态研究所 | Plant growth regulator and preparation method thereof |
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Application publication date: 20180710 |