CN1817140A - Plant-source growth regulator and use for improving crop growth - Google Patents
Plant-source growth regulator and use for improving crop growth Download PDFInfo
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Abstract
A vegetative growth regulator for increasing the yield of agricultural crops and improving their quality and the resistance to disease and pest is prepared from mugwort or shrubby flase indigo through destroying their tissue, immersing in organic solvent, extracting for 12-24 hr 2-3 times, concentrating the liquid extract, dissolving in organic solvent, adding emulsifier, stirring, and adding water.
Description
Technical field
The present invention relates to a kind of plant resource growth regulator (transient name), it is a raw material with argy wormwood (Arte misia argyi Levl.EtVant) or shrubby flase indigo (Amorphya fruticos) plant, a kind of low toxicity of development, efficient neoformation product, mode dispenser with foliage spray, be applied to farming, woods, animal husbandry field, the yield-increasing efficiency height can improve quality, noresidue is polluted, and has a extensive future.Meet the direction of agricultural chemicals development, but possessed the characteristics of advance, practicality generalization and the environmentally friendly machine of new product development, significant for development organic farming and green agriculture.
Background technology
At present, the main plant growth regulator that uses is chemosynthesis on agricultural both at home and abroad, as gibberellin, methyl, heteroauxin, and the basic element of cell division, CPPU etc.The growth regulator that derives from plant has the brassin lactones, jasmonic etc., but as product, still be to use chemosynthesis, can both significantly improve output, promote precocity, but most function ratio is more single, can not improve quality.If any medicine it is mixed with certain concentration, the fruit or the vegetables that soak to give birth to can make surface discolouration, seem ripe, interior flesh is still living, mouthfeel is very poor, is of low nutritive value, even also has certain toxicity.Residual medicine enters in the body, has side effects, grow as the teenager too fast, the side effect that early sexual maturity etc. bring, increasingly significant.In recent years, complex preparation is more, can solve to improve production problems, but still have the pollution to environment.So, the little biopesticide of research toxic and side effect is the direction and the development priority of research both at home and abroad, the external many advanced countries such as the U.S., Holland, Japan, Russia, France, Australia, Israel, India etc. greatly develop and use biopesticide, little by little use the biopesticide substituting chemical pesticide.This research product-plant resource growth regulator, the direct sample preparation of the mix ingredients that utilization is extracted from plant, made full use of the synergistic function of various active active principle in the plant corpus, make crop when significantly improving output, also improve the quality and quality, do not change original flavor, do not have the single hormone function of market chemosynthesis product, avoided the defective of the single component effect of chemosynthesis.This research product is a biologic product, and raw material is renewable, and product is easily degraded after use, and noresidue is polluted, and to protection environment, significant for raising human health, development organic farming and green agriculture, meets nuisanceless novel pesticide research direction fully.
Summary of the invention
One of purpose of the present invention provides a plant growth regulators, and this conditioning agent is all pollution-free in production and use, and product is easily degraded, and noresidue is polluted, and value of environmental protection height promotes the growth of crops, improves the yield and quality of crops.
Two of purpose of the present invention is the application aspect the promotion crop growth of this plant growth regulator, this conditioning agent has multi-functional and broad spectrum activity, is suitable for multiple farming, woods, animal husbandry crop, and growth-promoting functions is more comprehensive, be not subjected to geographical environment, crop varieties effects limit, the scope of application is extensive.This plant growth regulator can improve the yield and quality of crops effectively, simultaneously multiple damage by disease and insect is had certain function of preventing and treating, and also can strengthen crops opposing natural calamity ability.
The objective of the invention is to realize by following measure:
A kind of plant resource growth regulator, it is characterized in that it is a raw material with argy wormwood or shrubby flase indigo, with after the raw material disorganize (with juice extractor squeeze broken raw tissue or with other method disorganize), put into and extract still, add the heavy organic solvent (benzinum or hexane) of 2-2.5 times of raw material, room temperature condition soaks down and extracted 12-24 hour, so extracts 2-3 time; Extract is concentrated into solid shape concentrate.
Solid shape concentrate is dissolved in the organic solvent (benzinum or hexane) for its 0.5-1.5 times of weight, be incorporated as the doubly heavy emulsifier of solid shape concentrate 0.1-0.15 again, fully stir, be incorporated as the doubly heavy water of solid shape concentrate 80-120 again, promptly make the aqueous emulsion of plant resource growth regulator.
Described raw material is preferably selected the argy wormwood plant or the shrubby flase indigo plant of fresh wild or plantation for use;
During the raw material disorganize, there is juice to extrude, can earlier juice be collected, when adding water preparation aqueous emulsion, add.
Organic solvent is selected benzinum or all nonhazardouss of hexane (technical grade) (is that edible oil extracts solvent as hexane) for use.
Emulsifier is selected Tween-80, Tween-60 or polyoxyethylene glycerine 400 monostearates for use, all is to use the surfactant in food, all nonhazardouss.
Beneficial effect of the present invention:
Plant growth regulator of the present invention is the method that the mixture taking to extract from plant is directly made product, and production technology is simple, and flow process is short; Solvent can reclaim repeated use, greatly reduces production cost, easily industrialization, economic benefit height.
Plant growth regulator of the present invention is that the mixture that extracts from plant under the condition of gentle (below 50 ℃) is made, destroy less to the various active composition in the plant, in use, made full use of the synergy of multiple growth components in the plant, improved drug effect, reduce the toxic and side effect of single hormone, remedied the defective of the single hormone composition of market chemosynthesis effect.Reached the effect that improves the yield and quality.
Raw material of the present invention is shrubby flase indigo and argy wormwood (all containing part desinsection anthelmintic ingredient in two plant species), mixture extracting method in them is basic identical, the growth promotion effective ingredient of their extracts is basic identical, its growth promotion to crop has similar effect, and all crop disease and insect is had certain preventive and therapeutic effect.
Embodiment
Embodiment 1
Raw material: fresh wild or plantation argy wormwood plant.Organic solvent: hexane (technical grade).Emulsifier: Tween-80.
Bright argy wormwood cauline leaf 100kg is squeezed broken its tissue with juice extractor, extrude juice and reservation.Obtain the slag behind the juice, get slag and put into and extract still, add the hexane of 250kg, room temperature condition soaks down and extracted 12-24 hour, so extracts 2-3 time; With extract behind 50 ℃ of following temperature concentrating under reduced pressure, obtain solid shape concentrate 0.9kg, add 1 part of 1.3kg hexane, after dissolving under the water-bath heated and stirred, add the 0.09kg Tween-80, fully stir, add the juice that keeps, add entry again to 90kg, promptly make 1% plant resource growth regulator aqueous emulsion.
Embodiment 2
Raw material: fresh wild or plantation argy wormwood plant.Organic solvent: hexane (technical grade).Emulsifier: Tween-60.
Bright argy wormwood cauline leaf 100kg is squeezed broken its tissue with juice extractor, extrude juice and reservation.Obtain the slag behind the juice, get slag and put into and extract still, add the hexane of 250kg, room temperature condition soaks down and extracted 24 hours, so extracts 3 times; With extract behind 50 ℃ of following temperature concentrating under reduced pressure, obtain solid shape concentrate 1.0kg, add the 1.5kg hexane, after dissolving under the water-bath heated and stirred, add the 0.15kg Tween-60, fully stir, add the juice that keeps, add entry again to 80kg, promptly make 1.2% plant resource growth regulator aqueous emulsion.
Embodiment 3
Raw material: fresh wild or plantation argy wormwood plant.Organic solvent: benzinum (technical grade).Emulsifier: polyoxyethylene glycerine 400 monostearates.
Bright argy wormwood cauline leaf 100kg is squeezed broken its tissue with juice extractor, put into and extract still, add the 200kg benzinum, room temperature condition soaks down and extracted 15 hours, so extracts 2 times; With extract behind 50 ℃ of following temperature concentrating under reduced pressure, obtain solid shape concentrate 0.8kg, add the 0.45kg benzinum, after dissolving under the water-bath heated and stirred, add 0.085kg polyoxyethylene glycerine 400 monostearates, fully stir, add entry again, promptly make 0.8% plant resource growth regulator aqueous emulsion to 100kg.
Embodiment 4:
Raw material: fresh wild or plantation shrubby flase indigo plant.Organic solvent: benzinum (technical grade).Emulsifier: Tween-80.
Amorpha fruticosa leaf and stem 100kg are squeezed broken its tissue with juice extractor, extrude juice and keep.Obtain the slag behind the juice, get slag and put into and extract still, add the benzinum of 200kg, room temperature condition soaks down and extracted 20 hours, so extracts 3 times; With extract behind 50 ℃ of following temperature concentrating under reduced pressure, obtain the solid shape concentrate of 1.5kg, add the 1.0kg benzinum again, after dissolving under the water-bath heated and stirred, the Tween-80 that adds 0.15kg fully stirs, and adds the juice that keeps, add entry again to 150kg, promptly make 1% plant resource growth regulator aqueous emulsion.
Embodiment 5:
Raw material: fresh wild or plantation shrubby flase indigo plant.Organic solvent: benzinum (technical grade).Emulsifier: Tween-60.
Amorpha fruticosa leaf and stem 100kg are squeezed broken its tissue with juice extractor, extrude juice and keep.Obtain the slag behind the juice, get slag and put into and extract still, add the benzinum of 250kg, room temperature condition soaks down and extracted 24 hours, so extracts 2 times; With extract behind 50 ℃ of following temperature concentrating under reduced pressure, obtain the solid shape concentrate of 1.0kg, add the 1.5kg benzinum again, after dissolving under the water-bath heated and stirred, add the 0.1kg Tween-60, fully stir, add the juice that keeps, add entry again to 83kg, promptly make 1.2% plant resource growth regulator aqueous emulsion.
Embodiment 6:
Raw material: fresh wild or plantation shrubby flase indigo plant.Organic solvent: hexane (technical grade).Emulsifier: polyoxyethylene glycerine 400 monostearates.
Amorpha fruticosa leaf and stem 100kg are squeezed broken tissue with juice extractor or additive method, put into and extract still, add the hexane (technical grade) of 200kg, room temperature condition soaks down and extracted 20 hours, so extracts 2 times; With extract behind 50 ℃ of following temperature concentrating under reduced pressure, obtain the solid shape concentrate of 0.8kg, add 1.0kg hexane (technical grade) again, after dissolving under the water-bath heated and stirred, add 0.08kg polyoxyethylene glycerine 400 monostearates, fully stir, add entry again, promptly make 0.8% plant resource growth regulator aqueous emulsion to 100kg.
1. the main component of plant resource growth regulator, content and assay method:
The mixture content that with the argy wormwood is the raw material extraction after measured is that the main component of 1% plant resource growth regulator is: organic acid 200-300mg/L (neutralization titration mensuration), free amino acid 50-100mg/L (ninhydrin method mensuration), soluble sugar 500-800mg/L (anthrone method mensuration), general flavone 100-200mg/L (ultraviolet spectrophotometry), gibberellin 5-20mg/L (liquid chromatography), mitogen and heteroauxin 5-10mg/L (liquid chromatography) abscisic acid (ABA) 1-5mg/L etc.(also measuring with the shrubby flase indigo is that the mixture content that the raw material extraction is made is 1% plant resource growth regulator, and the above composition index content of surveying differs not obvious, provides in the data area above-mentioned basically).
2. the test of selecting materials
2.1 feverfew
Selecting fresh argy wormwood, cloudy old wormwood artemisia, chrysanthemum cinesariaefolium base of leaf respectively is raw material, extracts with above-mentioned 1 method, makes mixture content and be 1% aqueous emulsion.Test with the wheat germination rate and to choose raw material.It is 100mg.L that 1% aqueous emulsion of above-mentioned three plant species extracts is made into concentration respectively
-1Solution, stand-by.Choose plump-eared wheat (peaceful wheat No. 2) seed and be placed in the bottom and have in the culture dish (φ 12cm) of one deck filter paper, 50 in every ware, adding above-mentioned three plant species concentration respectively is 100mg.L
-1Solution 20ml, it is the soup of raw material that the 1-6 ware adds with the argy wormwood, the soup that it is raw material that the 7-12 ware adds cloudy old wormwood artemisia, 13-18 number adds chrysanthemum cinesariaefolium is the soup of raw material, adds clear water, is contrast for 18-24 number.Putting into 23-24 ℃ the biochemical incubator of TF-1A cultivates.Day by day add up germinative number, it is long to measure bud, and root is long, plant height, and root bar number is added up root dry weight at last, strain dry weight, root volume, root activity coefficient.Investigate 12 days altogether, every index determining method such as following table 1:
Table 1 argy wormwood, cloudy old wormwood artemisia, the test of chrysanthemum cinesariaefolium germination rate
| Handle | Germination rate % | The long cm of bud | The long cm of root | Plant height cm | Root dry weight mg | Strain dry weight mg | Root system activity m 2/ml | Root volume ml |
| The cloudy old wormwood artemisia chrysanthemum cinesariaefolium of clear water argy wormwood | 88 92 90 91 | 1.72 2.12 2.00 1.96 | 29.08 36.66 27.58 24.34 | 9.70 10.18 10.24 9.28 | 4.37 4.64 4.41 4.39 | 7.05 9.66 7.46 7.93 | 0.0020 0.0024 0.0024 0.0023 | 0.49 0.572 0.511 0.535 |
By table 1 statistics as can be seen, wheat germination rate measurement result, overall target, argy wormwood is better than other plant, is raw material so select argy wormwood.
Selecting argy wormwood, cloudy old wormwood artemisia, chrysanthemum cinesariaefolium respectively is raw material, extracts with said method, makes mixture content and be 1% aqueous emulsion.(spraying concentration is 100mg.L to be used for the wheat test
-1) show: being that the medicine rate of growth that raw material is made reaches 15% with the argy wormwood, is the highest rate of growth 10.5% of medicine that raw material is made with the old wormwood artemisia of the moon, is the highest rate of growth 8.6% of medicine that raw material is made with the chrysanthemum cinesariaefolium, and three kinds of raw material differences of prevention and elimination of disease and pests effect are not obvious.So selecting argy wormwood is that raw material ratio is better.
2.2 leguminous plant
Selecting shrubby flase indigo, yellowwood, acacia leaf respectively is raw material, measures identical method with above-mentioned with composite family, does the test of paddy rice germination rate, result such as following table 2:
Table 2 shrubby flase indigo, yellowwood, the test of acacia leaf germination rate
| Handle | Germination rate % | The long cm of bud | The long cm of root | Plant height cm | Root dry weight mg | Strain dry weight mg | Root system activity m 2/ml | Root volume ml |
| Clear water amorpha fruticosa leaf yellowwood leaf | 88.7 90.15 90.00 | 2.31 3.92 3.01 | 4.88 5.59 5.11 | 3.38 3.01 3.04 | 0.00303 0.00313 0.00300 | 0.00251 0.00309 0.00278 | 0.0013 0.0020 0.0017 | 0.28 0.30 0.30 |
| The acacia leaf | 90.00 | 3.00 | 5.14 | 3.12 | 0.00306 | 0.00269 | 0.0014 | 0.29 |
By table 2 statistics as can be seen, paddy rice germination rate measurement result, overall target, shrubby flase indigo is better than other plant.A lot of when in addition acacia and yellowwood borrow are taken a lot of work than shrubby flase indigo, the shrubby flase indigo aboundresources is shrub plant, easily pluck, but and continued growth after tapping, the north 1 year can borrow 2 times, and the 2-3 defective material can be adopted in south, is first-class selection so comparative result selects shrubby flase indigo as raw material.
Selecting shrubby flase indigo, yellowwood, acacia respectively is raw material, extracts with said method, makes mixture content and be 1% aqueous emulsion.(spraying concentration is 100mg.L to be used for the wheat test
-1) show: being the rate of growth 15.4% of the medicine made of raw material with the shrubby flase indigo, is the highest rate of growth 8.4% of the medicine made of raw material with the yellowwood, is the highest rate of growth 9.1% of the medicine made of raw material with the acacia, and prevention and elimination of disease and pests effect shrubby flase indigo is better than other plant.So it is first-class selection as raw material that comparative result selects shrubby flase indigo.
3 advantages of the present invention:
3.1 hypotoxicity, low-residual
The plant resource growth regulator derives from the extract in nonhazardous plant argy wormwood or the shrubby flase indigo, and formulated, sintetics non-chemically, after measured, chmice acute per os: LD
50＞20000mg/kg, rat acute per os: LD
50＞5000mg/kg belongs to the micro-virus kind agricultural chemicals, and all pollution-free in production and the use, product is easily degraded, and noresidue is polluted, value of environmental protection height.
3.2 the short function of giving birth to is remarkable
Growth-promoting functions is the major function of plant resource growth regulator, also is principal character.Test shows mainly and shows:
(1) after the seed soaking, strengthens seed vitality, improve germination vigor, germination rate, emergence rate.Strengthen root system development, have strong seedling, effect in strong sprout.Increase main root growth degree and secondary root, fibrous root bar number.Improve the seedling replanting survival rate, seedling-slowing stage shortens, and growing way is vigorous.(2) foliage spray.Generally, promote photosynthesis, increase chlorophyll and plant dry matter content, increase base portion neck bar rugosity, improve resistant to lodging and diseases and insect pests resistance.For cereal crops, promote effective tiller, branch, improve grouting speed, the percentage of earbearing tiller and grain number per spike, increase thousand corn weight and improve nutritive index, volume increase 11-18%.For vegetables, promotion is grown and fruit expands, precocity, and volume increase is at 12-26%.To fruit, improve fruit-setting rate, promote fruit to expand and painted and photosynthesis, improve single fruit weight, volume increase at 16-23%.Oil plant volume increase 10-14%, flue-cured tobacco volume increase 20%, and can improve nicotine content, the economic benefit height.
3.3 raising quality
Measuring shows that the plant resource growth regulator is influential to multinomial quality index, and compositions such as the sugar of multiple fruit, starch, amino acid, fat, cellulose, flavones are had in various degree raising respectively.Make increasing of grain, vegetables, fruit and cigarette quality generally, have higher commodity value.
3.4 diseases prevention, insect protected opposing natural calamity function
Field examination experiment shows that 1. the plant resource growth regulator has certain preventive and therapeutic effect to vegetables fusarium wilt, fruit scale disease, paddy rice rice warm disease, leaf class disease etc.2. various pests such as aphid, tortrix moth are prevented and treated function, make it be difficult for forming the large tracts of land insect pest.3. drought resisting, cold-resistant function.Fruit protected spend, bears fruit, very valuable to the slight frostbite of big paulin dish resisting cold alleviation.4. certain enhancing crop function resistant to lodging is arranged.
3.5 broad spectrum activity
Show that by extensive experimentation on various crop production-increasing function is in various degree all arranged, be applicable to various plants such as grain, vegetables, fruit, oil plant, flue-cured tobacco, herbage, flowers, applied range.Show that by the test of multizone multiple spot the plant resource growth regulator is not influenced by territorial environment, the South and the North all can use, and is better than like product.
3.6 comparison with like product
At present, the main like product of using is rue tire element lactones, gibberellin, methyl, the heteroauxin etc. that synthesize in the domestic production, and the plant resource growth regulator is that raw material is made with argy wormwood, shrubby flase indigo respectively in comparable situation such as the following table 3:(table 3)
The comparison of table 3 plant resource growth regulator and other conditioning agent
| Title | Action function | Toxicity |
| Gibberellin methyl α-naphthyl acetate heteroauxin brassin lactones plant source growth regulator | Promote to grow, germinate, break seed dormancy, improve fruit-setting rate.Irritation cell division and tissue differentiation, induced parthenocarpy prevents fruit drop, improves cold-resistant, saline-alkaline tolerance.Promote cell division, elongation, expansion, RNA to synthesize, induced parthenocarpy improves fruit-setting rate.Increase vegetation growth of plant, promote amphigamy, have cold-resistant, drought resisting, anti-salt action.Promote seed sprouting and well developed root system, promote to nourish and grow and reproductive growth, improve fruit-setting rate and fruit weight, quality, promote precocity, tiller to have diseases prevention, insect protected, drought resisting, anti-cold effect. | Chmice acute per os: LD 50＞25000mg/kg chmice acute per os: LD 50＞1456mg/kg mouse peritoneal injection: LD 50＞150mg/kg chmice acute per os: LD 50＞1456mg/kg chmice acute per os: LD 50＞20000mg/kg |
Show from the function comparative result: the plant resource growth regulator is more than above-mentioned synthetic product function, and toxicity compares less (gibberellin exception).
(the plant resource growth regulator is that raw material makes spraying medicine concentration: 100mg/L with the shrubby flase indigo to be compared as follows table 4 with the effect of increasing production of brassin lactones; Brassin lactones 0.05mg/L.)
The effect of increasing production of table 4 plant resource growth regulator and brassin lactones relatively
| The crop class | Brassin lactones effect of increasing production (%) | Plant resource growth regulator effect of increasing production (%) |
| Grain vegetables fruit oil plant flue-cured tobacco | 10-14 15-20 12-16 10-20 15-25 | 11-18 12-26 16-23 10-14 20-25 |
Comparative trial shows that the effect of increasing production of plant resource growth regulator and brassin lactones effect of increasing production differ not too big.And the plant resource growth regulator is biologic product, more meets environmental requirement.And quality is increased, and economic benefit and social benefit are good.
4. application benefit analysis
4.1 the city's capacity and the economic benefit of product
According to Chinese agriculture report in 2005, China's agricultural planting gross area was 153,553 thousand hectares in 2004, various chief crop areas, price statistics situation such as following table 5:
Table 5 China in 2004 agricultural planting situation statistics
| Kind | Cultivated area (thousand hectares) | Gross yield (ten thousand tons) | Price (unit/ton) |
| Paddy wheat and corn soybean oil plant vegetable and fruit | 28,379 21,62 25,446 9,589 14,431 17,560 9,76 | 17,909 9,195 13,029 1,740 3,066 15,341 | 2,288 1,554 232.4 3,256.9 10000 (estimations) 15000 (estimation) |
Studies show that, the about 0.3-0.4kg of mu dosage of plant resource growth regulator, if the cultivated area of grain, vegetables, fruit crop 10% use this product, year need ton surplus the dose 5800.As seen city's capacity is quite big.Through the effort in 3 to 5 years, open up the outer market of founding state, demand is bigger.According to this product characteristics, have tangible technical advantage and competitive strength, product also can be lasting, as can manufacture, and can be subjected to peasant's welcome, promise well.Through cost accounting, profit of sales 66.59%, economic benefit height.
4.2 society and environmental benefit analysis
The raw material of this product is directly adopted in wild argy wormwood, can not plant.Even plantation, do not need to take the good farmland yet, the contradiction of striving the field with grain not, utilize deserted mountain, Ye Po, river levee, roadside extensively to plant, both can satisfy the production needs of plant growth regulator, do not destroy ecotope, for agricultural development brings enormous benefits and motive force, again can greening environment, conserve water and soil, to keeping the ecological balance, purifying the human lives certain contribution is arranged.The product low toxicity, from raw material planting, production does not all produce the three wastes (slag after the extraction is also recycling), free from environmental pollution to using, and does not stay residual hazard.Has certain effect to improving human health.Thereby environment, soil are not had the effect of destruction yet, really accomplished innoxious plantation, production and use.The production cost is low, the rate of growth height, and indirect economic effect is remarkable.
5. the innovation point of product:
(1) is raw material directly first, studies successful plant resource growth regulator, opened up the new application of such plant with aboundresources, cheapness, the argy wormwood plant that is easy to get, shrubby flase indigo plant.
(2) adopt directly sample preparation of mixture (than extracting under the temperate condition) first, fully keep and utilized the synergistic function of the multiple short liveliness proof composition of inside plants, improved drug effect, reduced the toxic and side effect of single hormone.
(3) plant resource growth regulator product function many, use wide spectrum, obvious effect of increasing production, can improve quality, and multiple damage by disease and insect is had certain function of preventing and treating, also can strengthen crops opposing natural calamity ability, be better than the chemosynthesis product.
(4) plant resource growth regulating agent producing process is simple, and flow process is short, easily industrialization, and solvent can reclaim repeated use, and it is low to produce input cost, profit of sales height, remarkable in economical benefits.
(5) plant resource growth regulator product belongs to biologic product, and low toxicity, nuisanceless is for improving human health, improving the using value height of environment, significant for development organic farming and green agriculture.Echo and use situation:
6 effects
Plant resource growth regulator field trial was being carried out 5 years altogether inside and outside the province, and is annual in that the peripheral hardware test site is a plurality of inside the province.Mu surplus overall test, the demonstration area 20,000.The experimental field is divided into: organic agronomic crop, green crops, common crops.Being studied thing is five big classes: the grain class mainly contains: paddy rice, wheat, corn, malting barley.Greengrocery mainly contains: green soy bean, French bean, broccoli, cabbage, cucumber, tomato, Chinese cabbage, cabbage, capsicum etc.Fruits: mainly be apple.Oil plant class: rape, peanut.Tobacco: flue-cured tobacco.Test method is divided into two kinds of multiple multiple lab scale, demonstration tests.
6.1 the test effect on organic agronomic crop
Test site, Si Yuan organic food production base, Nanjing.
(1) test on organic vegetable (the plant resource growth regulator is that raw material is made with the argy wormwood)
Investigate by experiment and show that this product shows mainly effective pod is increased that bright pod weight increases, thereby forms volume increase.As French bean concentration is 100mg.L
-1Plant resource growth regulator foliage-spray after, on average become 18.75/strain of pod, the heavy 87.5g/ strain of bright pod becomes 15.5/strain of pod, the heavy 73.0g/ strain of bright pod and contrast is average.Handle comparison according to increasing by 3.25/strain and 4.5g/ strain respectively.
By being investigated, damage by disease and insect shows that the French bean gray mold is had certain control action.Invalid to the green soy bean insect.Output is investigated the result adds up in following subordinate list 1.
Subordinate list 1 plant resource growth regulator is in organic vegetable class result of the test
| Kind | Spraying medicine concentration mg.L -1 | Spraying times | Bright output Kg | Increase output Kg | Rate of growth % | Increment unit/mu | Unit price unit/Kg |
| French bean 75 green soy beans | 100 contrasts, 120 contrasts | 3 3 3 3 | 720.15 600.80 416.95 323.0 | 119.35 - 93.50 - | 19.87 - 29.09 - | 179.03 - 59.34 - | 1.50 - 2.04 - |
By output statistics as can be seen, it is 29.09% that this product makes organic fresh vegetables rate of growth, 179 yuan of mu increments, remarkable in economical benefits.Relevant attributional analysis result adds up in following subordinate list 2.
Subordinate list 2 organic vegetable attributional analysis results
| Project | French bean (fresh and tender product) | 75 green soy beans (fresh and tender dry product) | ||||
| mg/g | Handle | Contrast | Increase (±) | Handle | Contrast | Increase (±) |
| Soluble sugar starch flavones crude fibre aminoacid protein ash subtotaling | 0.685 6.237 62.68 0.302 69.904 | 0.151 8.009 45.71 0.314 54.18 | +0.534 -1.772 +16.97 -0.012 +15.72 | 4.99 3.50 0.1322 0.81 124.48 10.82 144.73 | 4.46 5.50 0.1545 1.06 85.27 11.37 107.82 | +0.53 -2.00 -0.0223 -0.25 39.21 -0.55 +36.92 |
From part index of quality analysis result, different indexs has to increase to have and subtracts, but total still increasing.(2) (this plant resource growth regulator is that raw material is made with the argy wormwood to the result of the test on organic paddy rice, foliage spray 3 times, concentration 100mg.L
-1)
By spraying 100mg.L in rice seedling phase, booting stage, fringe phase beginning
-1The plant resource growth regulator of concentration, investigation shows: the effect of tangible stimulation rice tillering is arranged.Handling comparison increases by 1.8 seedlings according to average every cave, and the every cave of peak seedling increases by 2.8 seedlings, and it is several 30.39 ten thousand to handle every mu of total seedling, and contrast is 25.72 ten thousand, mu increases seedling 4.67 ten thousand, increases seedling rate 15.37%.Fringe portion investigates the following subordinate list 3 of result:
Subordinate list 3 organic products paddy rice fringes are investigated result's statistics (average every cave)
| Handle mg.L -1 | Total grain number | Empty grain number | Real grain number | Ripening rate % | Cave dry weight g | Thousand kernel weight g |
| 100 compare | 112.4 115.4 -3.0 | 19.1 20.4 -1.3 | 93.3 95.0 -1.7 | 83.0 82.3 0.7 | 29.02 24.56 4.46 | 23.2 23.2 0 |
Find out that by subordinate list 3 thousand kernel weight of handling and contrasting does not change, but ripening rate, the cave dry weight handled improve obviously.In this experiment, owing to the influence of meeting typhoon and rice fulgorid disease at rice ear sprouting period, so certain influence is arranged for plant resource growth regulator field test effect.The output investigation result is added up in following subordinate list 4:
Subordinate list 4 organic products rice yields are investigated the result
| Handle mg.L -1 | Basic seedling ten thousand/mu | Gao Miaowan/mu | Spike number fringe/cave | Spike number fringe/mu | Actual production Kg | Rate of growth % |
| 100 compare | 8.35 8.35 | 30.39 25.72 4.67 | 14.2 12.2 2 | 23.71 20.37 14.08 | 960.2 820.3 148.9 | 15.36 |
Find out that by above experiment statistics result the plant resource growth regulator increases production by impelling organic products paddy rice effective tiller raising spike number.Relevant attributional analysis is added up in following subordinate list 5.
The quality determination of subordinate list 5 organic products paddy rice brown rice
| Project | Handle | Contrast | Increase (± %) |
| Total reducing sugar, (mg/g) soluble starch, (mg/g) amino acid, (mg/g) crude fat quality, (g) protein, (g) raw fiber, (g) add up to | 1.039 76.31 0.64 2.23 8.25 2.62 | 0.899 74.72 0.54 3.45 10.60 4.58 | +15.57 +1.59 +18.52 -1.22 -22.17 -1.95 +10.34 |
The result shows from attributional analysis, handle with respect to the every index increase and decrease of contrast difference, but overall performane numerical value has certain increase, shows that the plant resource growth regulator is to the rice quality effect of improving.
6.2 the test effect on green crops
(1) on malting barley result of the test (this plant resource growth regulator is that raw material is made with the argy wormwood, foliage spray 2 times, concentration 100mg.L
-1)
Shown by following test statistics: the plant resource growth regulator can promote plant growth, increases the crop dry-matter accumulation; Increase base portion neck bar rugosity, suppress the growth of basal internode, capacity for the resistance to lodging strengthens; Spike length, real grain number, thousand kernel weight increase, and empty flat grain reduces, and mu increases output 82kg, and input-output ratio increases.
Subordinate list 6 green product malting barley result of the tests statistics
| Project | Handle | Contrast | Increase (±) |
| Thick (cm) base portion first segment length (cm) base portion second section length (cm) base portion the 3rd joint length (cm) base portion of the real grain of the every fringe of Basic Seedling (ten thousand) peak seedling (ten thousand) mu Cheng Sui (ten thousand) number setting percentage (%) mass of 1000 kernel (g) per mu yield (kg) plant height (cm) base portion neck the 4th joint length (cm) base portion the 5th joint length (cm) individual plant economic flow rate (g) | 42.4 73.0 56.6 20.5 94.45 43.31 531.0 72.7 0.38 3.46 8.90 11.9 13.72 14.64 1.91 | 43.0 73.4 56.0 19.5 93.49 41.08 449.0 77.7 0.33 5.42 10.6 11.98 13.63 14.17 1.582 | -0.6 -0.4 +0.6 1.0 +0.96 +2.23 +82.0 -5.0 +0.05 -1.96 -1.70 -0.08 +0.09 +0.47 +0.228 |
(2) on flue-cured tobacco result of the test (this plant resource growth regulator is that raw material is made with the shrubby flase indigo, foliage spray 3 times, concentration 100mg.L
-1)
Find out that by table 7 statistics rate of growth is obvious, quality improves, considerable benefit.Show that by the field trial investigation this medicine can obviously promote the tobacco root prosperity, make tobacco leaf be fold, have the contrast of ripe spot many that exterior quality such as gloss are more intense.From part attributional analysis result, every index all has in various degree to be increased, and particularly nicotine content increases by 0.62%, most important to the quality that improves cigarette.
Subordinate list 7 green product flue-cured tobacco (NC-89) result of the tests statistics
| Project | Handle | Contrast | Increase (±) |
| Plant height (cm) but the roasting back of long (cm) * wide (cm) stem girth (cm) pitch (cm) of waist leaf cured leaf per mu yield (kg) is counted in picking leaves | 111.6 18.07 63.75×26.52 8.35 6.02 149.48 | 100.38 16.98 59.86×23.66 7.84 5.92 128.74 | +11.22 +1.09 +(4.89×2.86) 0.51 0.4 20.74 |
| Average price (upper medium grade cigarette (%) output value of unit/kg) (unit/mu) total reducing sugar (%) reduced sugar (%) total nitrogen (%) protein (%) nicotine (%) schmuck value | 7.5 85.05 1121.1 22.08 18.12 1.76 6.97 1.54 3.08 | 7.5 80.8 965.55 19.19 14.60 1.12 6.19 0.92 3.09 | +4.25 +155.55 +2.89 +3.96 +0.64 +0.78 0.62 -0.01 |
6.3 the test effect on common crops
The field trial investigation result is added up in table 8.(the plant resource growth regulator is that raw material is made with the argy wormwood in the table 8, foliage spray 3 times, concentration 100mg.L
-1) plant resource growth regulator raising cereal crops output, mainly increase production by effective tiller, branch, increase fringe (angle) grain number, thousand kernel weight.The first, increase the 1-2 grain as paddy rice percentage of earbearing tiller raising 3.8%, the equal grain of fringe number, fringe heavily increases 0.14g, and thousand kernel weight increases 0.8-1.4g.Rape is handled and is made effective branch increase 0.3-0.8, and individual plant angle fruit number increases 1.8-9.3, and seed number per pod increases 0.9, and thousand kernel weight increases 0.05g.Corn (No. 2, middle list) is handled 607 of grain number per spikes, and contrast is 600, and fringe increases 7, and thousand kernel weight increases 9.08g.The second, units increased in production is relevant with spraying times, and generally respectively relatively good with a medicine in three main vegetative period of crop, spraying times is few, the corresponding minimizing of rate of growth.The 3rd, relevant with spraying time, if spraying time not in three main vegetative period of crop, effect is not really remarkable.The 4th, from investigation, mainly be to promote to tiller and increase a number in growth dispenser in early stage, growth later stage dispenser mainly is that the influence grain is heavy.The 5th, some damage by disease and insect of crop there is certain preventive and therapeutic effect, certain cold-resistant drought resisting function is also arranged.
Test effect statistics on subordinate list 8 grains, the oil plant
| Time | The place | Project | Handle (kg/ mu) | Contrast (kg/ mu) | Increase (± %) |
| 2004 2005 2005 2004 2004 2005 2005 2005 | Yan'an, Nanjing, Yan'an, Nanjing, Nanjing, Weinan, Nanjing, Nanjing | Poplar wheat 158 wheat wheats (the black ton of moral) paddy rice 9520 paddy rice 9526 paddy rice 90-1 rape rapes | 362.05 475.69 465.0 670.5 693.7 443.7 835.1 143.1 | 306.8 409.46 400.0 612.5 622.8 387.0 743.4 126.5 | +18.0 +16.2 +16.25 +9.5 +11.38 +14.65 +12.35 +13.1 |
| 2005 2005 2005 | Yan'an, Yan'an, Yan'an | Peanut corn (No. 2, middle list) corn (single No. 2 of family) | 276.4 495.0 420.0 | 246.5 427.6 365.0 | +12.13 +15.76 +15.69 |
Test effect on vegetables, fruit is added up in following subordinate list 9.(table 9 plant resource growth regulator is that raw material is made with the shrubby flase indigo, foliage spray 3 times, concentration 100mg.L
-1)
Test effect statistics on subordinate list 9 vegetables, the fruit
| Time | The place | Project | Handle (kg/ mu) | Contrast (kg/ mu) | Increase (± %) |
| 2004 2004 2005 2005 2005 2005 2005 2004 2005 2005 | Yan'an, Yan'an, Yan'an, Yan'an, Yan'an, Yan'an, Yan'an, Yan'an, Nanjing, Nanjing | Cabbage autumn in onion summer cabbage autumn cabbage capsicum tomato cucumber apple (Fuji apple) apple (red marshal) apple (Qin Guan) | 2267.8 2038.4 6221.0 9010.0 4056.0 4168.5 1708.6 765.5 3212 4017 | 1800.9 1723.4 5555.6 8010.0 3440.0 3642.9 1439.4 659.4 2750 3250 | +26.0 +18.28 +11.98 +12.48 +17.91 +14.38 +18.94 +16.0 +16.8 +23.6 |
Part fruit, quality of vegetable analytic statistics are in subordinate list 10.
Subordinate list 10 fruit, cabbage attributional analysis result
| Kind | Cabbage | Apple (Fuji apple) | |||||
| Content (mg/g) | Handle | Contrast | Increase (±) | Content (%) | Handle | Contrast | Increase (±) |
| Soluble sugar starch crude fat raw fiber adds up to | 39.76 12.94 0.0189 0.0482 | 41.88 6.35 0.0357 0.0421 | -2.12 +6.59 -0.019 0.006 +4.45 | Soluble sugar total acid tinctorial yield hardness fruit shape index | 12.33 0.26 71.5 | 12.19 0.29 55.0 | + 0.14-0.03+16.5 reduce raising |
The result shows according to pilot survey, and the plant resource growth regulator mainly expands, improves single fruit weight and increase production by raising fruit-setting rate, promotion fruit on fruits and vegetables.Very low as the Hongyuanshuai apple fruit-setting rate, be the principal element that influences output,, can improve fruit-setting rate about 30% if spray the plant resource growth regulator one time at full-bloom stage, and have and necessarily resist the frost effect, this bears fruit extremely important in the north for keeping apricot, peach, apple.
Evidence on tomato and cucumber can improve fruit-setting rate 4%.In apple and the dispenser of vegetables fruit expanding period once, can significantly increase single fruit weight.In earlier stage spray medicine once at fruit maturation, obvious effect is arranged reducing scale and raising fruit top layer wax layer thickness and raising fruit gloss and sugar content.Vegetables spray medicine once before transplanting, can significantly shorten seedling-slowing stage and improve survival rate and to the resistivity of damage by disease and insect.
Claims (6)
1, a kind of plant resource growth regulator is characterized in that it is a raw material with argy wormwood or shrubby flase indigo, after the raw material disorganize, puts it into and extracts in the still, adds the heavy organic solvent of 2-2.5 times of raw material, and room temperature condition soaks down and extracted 12-24 hour, extracts 2-3 time; Extract is concentrated into solid shape concentrate, reclaims solvent;
Solid shape concentrate is dissolved in the organic solvent for its 0.5-1.5 times of weight, is incorporated as the doubly heavy emulsifier of solid shape concentrate 0.1-0.15, fully stir, be incorporated as the doubly heavy water of solid shape concentrate 80-120 again, promptly make the aqueous emulsion of plant resource growth regulator.
2, plant resource growth regulator according to claim 1 is characterized in that described raw material selects the argy wormwood plant or the shrubby flase indigo plant of fresh wild or plantation for use.
3, plant resource growth regulator according to claim 1 and 2 is characterized in that described organic solvent selects benzinum or hexane for use.
4, plant resource growth regulator according to claim 1 and 2 is characterized in that described emulsifier selects Tween-80, Tween-60 or polyoxyethylene glycerine 400 monostearates for use.
5, plant resource growth regulator according to claim 1 is characterized in that in the described raw material disorganize process, and the juice of extruding is collected and kept to get up, and adds when adding water preparation aqueous emulsion.
6, the application of the described plant resource growth regulator of claim 1 aspect the promotion crop growth.
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