CN107836291A - A kind of interplanting method of wing pod Cassia tora and khuskhus - Google Patents

A kind of interplanting method of wing pod Cassia tora and khuskhus Download PDF

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Publication number
CN107836291A
CN107836291A CN201711134702.9A CN201711134702A CN107836291A CN 107836291 A CN107836291 A CN 107836291A CN 201711134702 A CN201711134702 A CN 201711134702A CN 107836291 A CN107836291 A CN 107836291A
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khuskhus
cassia tora
wing pod
pod cassia
parts
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CN107836291B (en
Inventor
易显凤
姚娜
丘金花
赖志强
梁永良
曾繁泉
黄志朝
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Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
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Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00—Methods for working soil
    • A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
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    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
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    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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    • A01N65/08—Magnoliopsida [dicotyledons]
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    • A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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    • A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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Abstract

The present invention relates to planting technology field, the interplanting method of more particularly to a kind of wing pod Cassia tora and khuskhus, the present invention is interplanted using khuskhus with wing pod Cassia tora, and the purpose for improving cadmium content in soil is reached using the superpower rich cadmium ability of khuskhus；The application is also reached for soil using the superpower nitrogen fixing capacity of wing pod Cassia tora and provides enough nitrogens simultaneously, ensure that khuskhus growth carries out symbiosis with wing pod Cassia tora, the application pre-processes to khuskhus branch and wing pod Cassia tora seed, the rooting rate of wing pod Cassia tora germination percentage and khuskhus can be greatly improved, effectively facilitate the growth of wing pod Cassia tora and khuskhus, reach wing pod Cassia tora and khuskhus symbiosis, accelerate supplement soil fertility so as to reach, further improve the purpose of cadmium content in soil.

Description

A kind of interplanting method of wing pod Cassia tora and khuskhus
【Technical field】
The present invention relates to planting technology field, the interplanting method of more particularly to a kind of wing pod Cassia tora and khuskhus.
【Background technology】
In recent years, because industrial activity is more and more frequent, the pollution that soil is subject to is increasingly severe, and cadmium is that human body is nonessential Element, often exist in nature with compound state, general content is very low, under home state, does not interfere with human body and is good for Health, after environment is by cadmium pollution, cadmium can be enriched with vivo, cause slow poisoning, cadmium quilt into human body by food chain After absorption of human body, cadmium sulfoprotein is formed in vivo, is optionally accumulated in liver, kidney, wherein, kidney is absorbable to enter internal nearly 1/ 3 cadmium, it is that cadmium poisoning " target organ ", other internal organs such as spleen, pancreas, thyroid gland and hair etc. also has a certain amount of accumulation；Due to Cadmium damages renal tubule, and glycosuria, albuminuria and acidaminuria occurs in patient.Especially tool makes the metabolism of bone be obstructed, and causes sclerotin to dredge The a series of symptoms such as pine, atrophy, deformation.It has been investigated that legume can effectively improve the accumulation ability of cadmium, for example, application Number be 201019050049.7, application it is entitled《A kind of method using mesquite remediating heavy metal cadmium pollution soil》Just have Utilize wing pod Cassia tora (scientific name：Cassia alata) it is planted in the soil of cadmium pollution, reach restoring cadmium polluted purpose, but Be through applicants have found that, only plant wing pod Cassia tora can't quick rich cadmium, it is restoring cadmium polluted.
Khuskhus (Stylosanthes) also known as the energy fast-growth such as Brazilian clover, tropical clover, also there is certain reparation cadmium The ability of pollution, it is the herbage that a kind of south is planted extensively；Its growth ability is extremely strong, but khuskhus growth ability is vigorous, no , if khuskhus and wing pod Cassia tora can be carried out into symbiosis, can quickly it improve the problem of cadmium pollution with other plant symbiosis.Together When, wing pod Cassia tora has very strong nitrogen fixing capacity, can effectively supplement soil fertility, further improve soil property.
【The content of the invention】
In view of the above, it is necessary to a kind of wing pod Cassia tora and khuskhus interplanting method are provided, can accomplish wing pod Cassia tora and Khuskhus symbiosis, so as to reach rehabilitating soil cadmium pollution, soil fertility is effectively supplemented, further improve the purpose of soil property.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
The interplanting method of a kind of wing pod Cassia tora and khuskhus, methods described comprise the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1kg/m2-2kg/m2Applied amount thrown into pre- kind ground Antivirus powder is spilt, according to 500g/m2-800g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, cover Mulch film is opened after 20d-30d, then applies organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 1kg/m2-2kg/m2, fertilising Obtain planting plot by 15d-20d curing afterwards；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, by wing pod Cassia tora seed and sterilization Liquid is 1 according to mass ratio:2-3 is mixed, and is washed after soaking 5min-10min with distilled water, by wing pod Cassia tora seed after washing With seed-soaking liquid according to mass ratio be 1:3-4 soaks 24h-26h in the case where temperature is 25 DEG C -30 DEG C of constant temperature, is carried out after immersion Filtering, is uniformly placed in culture cup after seed is drained, and is 25 DEG C -30 DEG C in temperature, relative humidity 10%- 15%, intensity of illumination is cultivated under conditions of being 800Lx-1000Lx, until growing the high wing pod Cassia tora seedling of 5cm-7cm；It is described The mode of washing of distilled water is：Wing pod Cassia tora seed is filtered to taking-up from thimerosal, then according to wing pod Cassia tora and distilled water Mass ratio is 1:2 mixing, distilled water is discarded after stirring 1min, then by wing pod Cassia tora and distilled water mass ratio is 1:2 mixing, stir Mix, so wash 5-7 times repeatedly；
(3) wing pod Cassia tora is planted：Plant by the wing pod Cassia tora seedling of step (2) according to 30cm-40cm × 30cm-40cm Away from bunch planting on the plantation plot of step (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 20%-30%；
(4) khuskhus branch pre-processes：Clip length is 10cm-15cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1-2 branch, 2-3 piece blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch is grown The khuskhus branch pre-processed is obtained during the coring of 2cm-5cm length；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 30d-40d, khuskhus that step (4) has been pre-processed Branch is colonized between two adjacent row wing pod Cassia toras, and according to 500g/m2-600g/m2The amount of spraying into whole plantation plot Foliar fertilizer is sprayed, is spaced after 15d-17d according still further to the same amount of spraying sprinkling once.
Further, step (1) the antivirus powder comprises the following components in parts by weight：15 parts -25 parts of calcium oxide, 8 Parts -13 parts of eucalyptus extracts, 9 parts -15 parts of Artemisinin, 7 parts -13 parts of chrysanthemum root bark extract and 5 parts -11 parts Chitosan.
Further, step (1) the organic fertilizer I is comprised the following components in parts by weight：5 parts -10 parts of vermiculite, 15 The manioc waste, 13 parts -23 parts of mulberry leaf ferment, 14 parts -26 parts of soybean peel and 23 parts -35 parts of peanut press pulp of -25 parts of part.
Further, step (2) thimerosal is made up of the composition of following percentage by weight：0.1%-0.3%'s HgCl2, 1%-3% aloe extract, 2%-5% extract of willow bark, surplus be water.
Further, step (2) seed-soaking liquid is made up of the composition of following percentage by weight：1%-3% permanganic acid Potassium, 1%-3% gibberellin, 1%-3% sodium chloride, 1%-3% Abelmoschus Esculentus Linn extract, surplus are water.
Further, nutrient solution is also equipped with step (2) culture cup, the nutrient solution is by following percentage by weight Composition forms：5%-7% manioc waste soak I, 2%-5% trehalose, 1%-3% spirulina extract, 1%-3% Stems of dragon fruits extract, surplus be water.
Further, step (4) the branch processing water is made up of the composition of following percentage by weight：5%-7% wood Potato slag soaking solution II, 2%-5% humic acid, 1%-3% heteroauxin, 1%-3% extract of soybean, surplus are water.
Further, step (5) foliar fertilizer is made up of the composition of following percentage by weight：5%-7% urine, 2%-5% potassium nitrate, 1%-3% sodium dihydrogen phosphate, 1%-3% extract of soybean, surplus are water.
Gibberellin used in this application is (GA3).
The present invention has the advantages that：
1st, the present invention can realize that the interplanting of khuskhus and wing pod Cassia tora, symbiosis reach and utilize the superpower fixed nitrogen energy of wing pod Cassia tora Power and be reached for soil and enough nitrogen be provided, ensure to accomplish and khuskhus symbiosis, energy in the case of khuskhus growth Being reached using the superpower rich cadmium ability of khuskhus improves the purpose of cadmium content in soil；Different from general planting, the application exists Plantation plot is carried out disinfection using disinfectant powder in advance before plantation, can effectively kill and be not suitable for having for white flower French beans growth in soil Evil pathogenic bacteria, antivirus powder are made up of calcium oxide, eucalyptus extracts, Artemisinin, chrysanthemum root bark extract and chitosan, are formulated In except calcium oxide is inorganic matter, other is all organic matter extract, except active composition itself can kill it is pathogenic micro- Biology plays a part of outside antivirus, turned in the later stage by soil bury, the effect such as stack retting can also be decomposed into organic matter, will not be to environment Pollute；The application applies organic fertilizer I, organic fertilizer I is by leech in planting process in advance according to the growth characteristics of wing pod Cassia tora Stone, manioc waste, mulberry leaf ferment, soybean peel and peanut press pulp composition, wherein, except having containing abundant in manioc waste, mulberry leaf ferment There is abundant probiotics outside machine matter, can effectively decompose the macromolecular substances in organic fertilizer I, particularly vegetable protein, will can have Machine thing is converted into small-molecule substance, more conducively plant absorption, contains abundant protein in soybean peel and peanut press pulp, is organic fertilizer The main source of nitrogen source, vermiculite improve the effect of organic fertilizer porosity during organic fertilizer fermentation, increase organic fertilizer I porositys Greatly, promote decomposition of the microorganism to organic fertilizer I, improve soil property before being adapted to plantation.
2nd, the application also pre-processes to the seed of wing pod Cassia tora, using thimerosal to wing pod Cassia tora seed in pretreatment Carry out disinfection；Thimerosal is by HgCl2, aloe extract, extract of willow bark composition, in thimerosal in addition to containing Hg, also contain There are abundant barbaloin, aloe polysaccharide, bigcatkin willow glucoside and tannin constituents, broad-spectrum bactericidal action can be played, can effectively kill wing pod Harmful microorganism in Cassia seed；Invention is also pre-processed with seed-soaking liquid to wing pod Cassia tora seed, and seed-soaking liquid is by permanganic acid Potassium, gibberellin, sodium chloride and Abelmoschus Esculentus Linn extract composition；Wherein potassium permanganate, gibberellin, okra polysaccharide, Flavonoid substances The conversion ratio of wing pod Cassia tora seed organic matter can be effectively improved, the germination percentage of wing pod Cassia tora seed can be effectively improved；Wing pod Cassia tora Nutrient solution is contained by vitamin C in manioc waste soak I, trehalose, spirulina extract, stems of dragon fruits extract, dragon fruit is more Sugar can significantly improve the growth ability of wing pod Cassia tora seed.
3rd, the application also pre-processes to khuskhus, and khuskhus are located in advance using branch processing water in pretreatment Reason, branch processing water are made up of manioc waste soaking solution II, humic acid, heteroauxin, extract of soybean；Wherein, manioc waste soaks Liquid II, humic acid can provide fertility for khuskhus, and heteroauxin can promote the conversion of Stylo seed, total Huang of extract of soybean Ketone, amino acids material can effectively improve the rootability of khuskhus；The application is also applied fertilizer using foliar fertilizer to khuskhus, Foliar fertilizer is made up of general flavone, amino acids thing in extract of soybean urine, potassium nitrate, sodium dihydrogen phosphate, extract of soybean Matter can effectively improve the growth ability of khuskhus, and it can be that khuskhus carry that wherein urine can provide N element, potassium nitrate for khuskhus P element can be provided for N, K element, sodium dihydrogen phosphate for khuskhus.
【Embodiment】
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive Feature and/or step beyond, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated otherwise, each Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1：
The interplanting method of a kind of wing pod Cassia tora and khuskhus, methods described comprise the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1kg/m2Applied amount shed antivirus into pre- kind ground Powder, according to 500g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, open ground after covering 20d Film, then apply organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 1kg/m2, planted after fertilising by 15d curing Plant plot；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, by wing pod Cassia tora seed and sterilization Liquid is 1 according to mass ratio:2 mixing, are washed after soaking 5min with distilled water, by wing pod Cassia tora seed and seed-soaking liquid after washing It is 1 according to mass ratio:3 soak 24h in the case where temperature is 25 DEG C of constant temperature, are filtered after immersion, after seed is drained Even is placed in culture cup, and is 25 DEG C in temperature, and relative humidity 10%, intensity of illumination is cultivated under conditions of being 800Lx, Until growing the high wing pod Cassia tora seedling of 5cm；The mode of washing of the distilled water is：By wing pod Cassia tora seed from thimerosal mistake Leaching goes out, and is then 1 according to wing pod Cassia tora and distilled water mass ratio:2 mixing, distilled water is discarded after stirring 1min, then by wing pod Cassia is 1 with distilled water mass ratio:2 mixing, stirring, are so washed 5-7 times repeatedly；
(3) wing pod Cassia tora is planted：By the wing pod Cassia tora seedling of step (2) according to 30cm × 30cm seeding row spacing bunch planting in step Suddenly on the plantation plot of (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 20%；
(4) khuskhus branch pre-processes：Clip length is 10cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1 point Branch, 2 blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch grows the root of 2cm length The khuskhus branch pre-processed is obtained during palpus；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 30d, khuskhus branch that step (4) has been pre-processed It is colonized between two adjacent row wing pod Cassia toras, and according to 500g/m2The amount of spraying spray foliar fertilizer into whole plantation plot, It is spaced after 15d according still further to the same amount of spraying sprinkling once.
Wherein, step (1) antivirus powder comprises the following components in parts by weight：15 parts of calcium oxide, the extraction of 8 parts of eucalyptus Thing, 9 parts of Artemisinin, 7 parts of chrysanthemum root bark extract and 5 parts of chitosan.
1. the extracting method of eucalyptus extracts is as follows in powder of killing virus：By moisture content be 5% eucalyptus leaves and press bark according to Mass ratio is 1:Crush after 2 mixing and screened by 100 eye mesh screens, is then divided into two parts powder, a copy of it is put into 3 times of quality Part, refluxing extraction 22h in the butanol solution that butanol percentage by volume is 20%, filter to take solvent be evaporated under reduced pressure, dry it is straight To moisture content eucalyptus extracts I is obtained for 3%；The oil that another is put into 4 times of mass parts, petroleum ether percentage by volume is 10% Refluxing extraction 24h in ethereal solution, filter to take solvent and be evaporated under reduced pressure, dried until moisture content is 3% to obtain eucalyptus extracts II.In this implementation eucalyptus extracts, ter penoidses content is 45.36mg/g, flavones ingredient content is 187.14mg/g, tannin Component content is 25.87mg/g, phloroglucinol derivatives component content is 121.32mg/g, glycoside component content is 109.76mg/ g。
2. the extracting method of Artemisinin is as follows in powder of killing virus：Fresh sweet wormwood is smashed to pieces, then adds 3 times of mass parts, The ethanol solution that ethanol percentage by volume is 80% is uniformly mixed, and mixture is put into supersonic extractors and carries out ultrasonic extraction, Ultrasonic extraction power is 20kHz, 90W ultrasonic wave, and Extracting temperature is 50 DEG C, after single effect 20min, stop 1min being further continued for into Row single acts on 20min, repeats single and acts on 5 times, filters to take filtrate progress rotary evaporation concentrate drying until moisture content is 4% Artemisinin is obtained, qinghaosu composition is 87.88% in extract.
3. the extracting method of chrysanthemum root bark extract is as follows in powder of killing virus：Chrysanthemum root skin is dried, until moisture content is 4%, it is then 1 according to mass ratio by chrysanthemum root and chrysanthemum skin:Crush after 2 mixing and screened by 40 eye mesh screens, then powder is divided Into two parts, refluxing extraction 30h, filtering in the butanol solution that a copy of it is put into 3 times of mass parts, butanol percentage by volume is 25% Take solvent to be evaporated under reduced pressure, dry until moisture content obtains chrysanthemum root bark extract I for 3%；Another is put into 6 times of quality Part, refluxing extraction 32h in the acetone soln that acetone percentage by volume is 10%, filter to take solvent be evaporated under reduced pressure, dry it is straight To moisture content chrysanthemum root bark extract II is obtained for 3%.In the present embodiment chrysanthemum root bark extract, patuletin -3-O- grapes Glycosides Contents are 361.24mg/g, flavones ingredient content is 518.03mg/g.
4. the collocation method of antivirus powder is：By calcium oxide, eucalyptus extracts, Artemisinin, chrysanthemum root bark extract and Chitosan screens by 200 eye mesh screens respectively, then weighs each composition by above-mentioned parts by weight and is produced again by mixing.
Wherein, step (1) organic fertilizer I is comprised the following components in parts by weight：5 parts of vermiculite, 15 parts of manioc waste, 13 parts Mulberry leaf ferment, 14 parts of soybean peel and 23 parts of peanut press pulp.
1. manioc waste source is in organic fertilizer I：The waste residue obtained after Cassava alcohol fermentation, the content of organics in manioc waste 90% is can reach, wherein, COD contents are 200mg/L.
2. mulberry leaf ferment is in organic fertilizer I：Product of the mulberry leaf after probiotics fermention, new fresh mulberry leaf is put into temperature is 25 DEG C, relative humidity is to carry out stack retting in 15% ferment at constant temperature room, is sprayed into mulberry leaf according to 20mg/g addition during stack retting Sucrose water is spilt, the wherein concentration of sucrose water is 3mol/L；After stack retting 10d, dry ferment is added according to 5mg/g addition into mulberry leaf Mother, above-mentioned mulberry leaf ferment is obtained after fermenting cellar is sealed by fermentation into 40d after stirring.
3. organic fertilizer I production method is：Weighed by the parts by weight and mixture is put into temperature after each raw material is well mixed Spend for 28 DEG C, relative humidity is to be fermented in 15% anaerobic fermentation room, fermentation time 10d.
Wherein, step (2) thimerosal is made up of the composition of following percentage by weight：0.1% HgCl2, 1% aloe carry It is water to take thing, 2% extract of willow bark, surplus.
1. the extracting method of aloe extract is in thimerosal：Fresh aloe belt leather is pulverized, then according to aloe and second Alcohol mass ratio is 1:4 add the ethanol solution that percentage by volume is 70%, and 24h is stood in the case where temperature is -3 DEG C of freezing conditions, Then ultrasonic extraction is carried out in supersonic extractors are put into, ultrasonic extraction power is 500w, and Extracting temperature is 50 DEG C, extraction time For 30min, place into refluxing extraction is carried out in refluxing extraction device afterwards, Extracting temperature is 100 DEG C, extraction time 6h, finally Filter to take filtrate and carry out rotary evaporation concentration, drying, until moisture content obtains aloe extract for 3%.Barbaloin in extract Content is 132.45mg/g；Aloe polysaccharide content is 245.98mg/g.
2. the extracting method of extract of willow bark is in thimerosal：By willow bark dry until bark moisture content be 5%, Then willow bark crushed, cross 100 mesh sieves and obtain willow bark powder, according to mass ratio be 1 by powder and ethanol solution:6 are carried out Mixing stands 24h, and the wherein percentage by volume of ethanol solution is 95%；After standing, mixture is put into refluxing extraction device Row refluxing extraction, reflux extracting time 12h, solvent is filtered to take afterwards and is evaporated under reduced pressure, dried until moisture content obtains for 3% To chrysanthemum root bark extract II.In extract, the content of bigcatkin willow glucoside is 541.26mg/g, tannin constituents content is 42.03mg/ g。
3. the collocation method of thimerosal is：HgCl is weighed by above-mentioned percentage by weight2, aloe extract, willow bark carry Thing and water is taken to be mixed, constant temperature stirring 2min is cooled to room temperature and is sterilized liquid after being heated slowly to 50 DEG C.
Wherein, step (2) seed-soaking liquid is made up of the composition of following percentage by weight：1% potassium permanganate, 1% it is red mould Element, 1% sodium chloride, 1% Abelmoschus Esculentus Linn extract, surplus are water.
1. the extracting method of Abelmoschus Esculentus Linn extract is in seed-soaking liquid：Fresh okra belt leather is pulverized, then according to the yellow autumn Certain herbaceous plants with big flowers is 1 with ethanol mass ratio:5 add the ethanol solution that percentage by volume is 95%, quiet in the case where temperature is -3 DEG C of freezing conditions 12h is put, ultrasonic extraction is then carried out in supersonic extractors are put into, ultrasonic extraction power is 800w, and Extracting temperature is 60 DEG C, is carried It is 50min to take the time, places into refluxing extraction is carried out in refluxing extraction device afterwards, and Extracting temperature is 95 DEG C, extraction time 8h, Finally filter to take filtrate and carry out rotary evaporation concentration, drying, until moisture content obtains Abelmoschus Esculentus Linn extract for 3%.In extract Okra polyoses content is 368.32mg/g；Flavonoid substances content is 465.54mg/g.
2. the collocation method of seed-soaking liquid is：Potassium permanganate, gibberellin, sodium chloride, Huang are weighed by above-mentioned percentage by weight Gumbo extract and water are mixed, and constant temperature stirring 2min is cooled to room temperature and obtains seed-soaking liquid after being heated slowly to 60 DEG C.
Wherein, it is also equipped with nutrient solution in step (2) culture cup, the nutrient solution is by following percentage by weight into packet Into：5% manioc waste soak I, 2% trehalose, 1% spirulina extract, 1% stems of dragon fruits extract, surplus For water.
1. manioc waste soak I immersion process is in nutrient solution：The waste residue of cassava fermentation wine-making essence is dried to and contained It is 2 according to the mass ratio of manioc waste and distilled water after water rate is 10%:1 is mixed, is heated to 60 DEG C and carries out constant temperature immersion Manioc waste soak I is obtained after 48h, filtering.In soak, the content of organic matter can reach 534.32mg/g.
2. the extracting method of spirulina extract is in nutrient solution：According to mass ratio it is 1 by spirulina and ethanol:8 and body The ethanol that product percentage is 95% is mixed, and 8h is stood in the case where temperature is -3 DEG C of freezing conditions, is then carried being put into ultrasound Take and ultrasonic extraction is carried out in device, ultrasonic extraction power is 800w, and Extracting temperature is 60 DEG C, extraction time 60min, is then carried out Filter to take filtrate and carry out rotary evaporation concentration, drying, until moisture content obtains spirulina extract for 3%.Flax in extract Oleic-acid content of material is 416.28mg/g；Vitamin content is 95.54mg/g
3. the extracting method of stems of dragon fruits extract is in nutrient solution：It is ground after stems of dragon fruits is peeled, then presses It is 1 according to stems of dragon fruits and ethanol mass ratio:4 add the ethanol solution that percentage by volume is 70%, in the freezing that temperature is -3 DEG C Under the conditions of stand 18h, then Extracting temperature be 100 DEG C under conditions of constant temperature carry out refluxing extraction, extraction time 6h, finally Filter to take filtrate and carry out rotary evaporation concentration, drying, until moisture content obtains aloe extract for 3%.Vitamin C in extract Content is 154.25mg/g；Dragon fruit polyoses content is 365.25mg/g.
4. the collocation method of nutrient solution is：Manioc waste soak I, trehalose, spiral are weighed by above-mentioned percentage by weight Algae extract, stems of dragon fruits extract and water are mixed, and constant temperature stirring 2min is cooled to room temperature and obtained after being heated slowly to 55 DEG C To nutrient solution.
Wherein, step (4) branch processing water is made up of the composition of following percentage by weight：5% manioc waste soaking solution II, 2% humic acid, 1% heteroauxin, 1% extract of soybean, surplus are water.
1. the immersion process of manioc waste soaking solution II is in branch processing water：The waste residue of cassava fermentation wine-making essence is dried It is 1 according to manioc waste and alcohol quality ratio after being 10% to moisture content:1, which adds the alcohol that percentage by volume is 75%, stirs 1min is cleaned, filtering drying remove alcohol after according to the mass ratio of manioc waste and distilled water be 3:1 is mixed, is heated to 60 DEG C and carry out constant temperature immersion 54h, filtering after obtain manioc waste soaking solution II.In soak, the content of organic matter can reach 754.03mg/g。
2. the extracting method of extract of soybean is in branch processing water：Soybeans soaking is stayed overnight, after filtering according to soybean with The mass ratio of water is 1:1 is mixed, and mixed liquor is obtained after grinding, by the ethanol solution that mixed liquor and percentage by volume are 95% It is 1 according to mass ratio:1 is mixed, and then carries out Microwave Extraction, and Microwave Extraction power is 1000w, and temperature is 90 DEG C, extraction Time is 3h, is filtered off afterwards except filter residue, takes filtrate to carry out rotary evaporation concentration, drying, until moisture content obtains soybean for 3% Extract, general flavone content is 340.34mg/g in extract, and amino acids material total content is 213mg/g.
3. the collocation method of branch processing water is：By above-mentioned percentage by weight weigh manioc waste soaking solution II, humic acid, Heteroauxin, extract of soybean and water are mixed, and constant temperature stirring 2min is cooled to room temperature and obtains branch after being heated slowly to 55 DEG C Bar handles water.
Wherein, step (5) foliar fertilizer is made up of the composition of following percentage by weight：5% urine, 2% potassium nitrate, 1% sodium dihydrogen phosphate, 1% extract of soybean, surplus are water.
1. the source of urine is in foliar fertilizer：It is 1 according to urine and water quality ratio after people, animal urine stoste is mixed:3 After being mixed, being filtered to remove impurity, filtrate is taken to obtain urine in foliar fertilizer.
2. the extracting method of extract of soybean is in foliar fertilizer：Soybeans soaking is stayed overnight, according to soybean and water after filtering Mass ratio is 1:1 is mixed, and mixed liquor is obtained after grinding, by ethanol solution that mixed liquor and percentage by volume are 95% according to Mass ratio is 1:1 is mixed, and then carries out Microwave Extraction, and Microwave Extraction power is 1000w, and temperature is 90 DEG C, extraction time For 3h, filter off afterwards except filter residue, take filtrate to carry out rotary evaporation concentration, drying, until moisture content obtains soybean extraction for 3% Thing, general flavone content is 340.34mg/g in extract, and amino acids material total content is 213mg/g.
3. the collocation method of foliar fertilizer is：Manioc waste soaking solution II, humic acid, indoles are weighed by above-mentioned percentage by weight Acetic acid, extract of soybean and water are mixed, and constant temperature stirring 2min is cooled to room temperature and is sterilized liquid after being heated slowly to 55 DEG C
Embodiment 2：
The interplanting method of a kind of wing pod Cassia tora and khuskhus, methods described comprise the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 2kg/m2Applied amount shed antivirus into pre- kind ground Powder, according to 800g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, open ground after covering 30d Film, then apply organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 2kg/m2, planted after fertilising by 20d curing Plant plot；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, by wing pod Cassia tora seed and sterilization Liquid is 1 according to mass ratio:3 mixing, are washed after soaking 10min with distilled water, by wing pod Cassia tora seed and seed soaking after washing Liquid is 1 according to mass ratio:4 soak 26h in the case where temperature is 30 DEG C of constant temperature, are filtered after immersion, after seed is drained Uniformly it is placed in culture cup, and is 30 DEG C in temperature, relative humidity 15%, intensity of illumination is trained under conditions of being 1000Lx Support, until growing the high wing pod Cassia tora seedling of 7cm；The mode of washing of the distilled water is：By wing pod Cassia tora seed from thimerosal Middle filtering is taken out, and is then 1 according to wing pod Cassia tora and distilled water mass ratio:2 mixing, distilled water is discarded after stirring 1min, then will Wing pod Cassia tora is 1 with distilled water mass ratio:2 mixing, stirring, are so washed 7 times repeatedly；
(3) wing pod Cassia tora is planted：By the wing pod Cassia tora seedling of step (2) according to 40cm × 40cm seeding row spacing bunch planting in step Suddenly on the plantation plot of (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 30%；
(4) khuskhus branch pre-processes：Clip length is 15cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 2 points Branch, 3 blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch grows the root of 5cm length The khuskhus branch pre-processed is obtained during palpus；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 40d, khuskhus branch that step (4) has been pre-processed It is colonized between two adjacent row wing pod Cassia toras, and according to 600g/m2The amount of spraying spray foliar fertilizer into whole plantation plot, It is spaced after 17d according still further to the same amount of spraying sprinkling once.
Wherein, step (1) antivirus powder comprises the following components in parts by weight：25 parts of calcium oxide, the extraction of 13 parts of eucalyptus Thing, 15 parts of Artemisinin, 13 parts of chrysanthemum root bark extract and 11 parts of chitosan.
(the extraction of the extracting method, the extracting method of Artemisinin, chrysanthemum root bark extract of antivirus powder eucalyptus extracts Method, the collocation method for powder of killing virus and embodiment 1 are completely the same.)
Wherein, step (1) organic fertilizer I is comprised the following components in parts by weight：10 parts of vermiculite, 25 parts of manioc waste, 23 The mulberry leaf ferment, 26 parts of soybean peel and 35 parts of peanut press pulp of part.
(manioc waste source, mulberry leaf ferment preparation method, organic fertilizer I production method and embodiment 1 are complete in organic fertilizer I Unanimously.)
Wherein, step (2) thimerosal is made up of the composition of following percentage by weight：0.3% HgCl2, 3% aloe carry It is water to take thing, 5% extract of willow bark, surplus.
(extracting method of aloe extract, the extracting method of extract of willow bark, the collocation method of thimerosal in thimerosal It is completely the same with embodiment 1.)
Wherein, step (2) seed-soaking liquid is made up of the composition of following percentage by weight：3% potassium permanganate, 3% it is red mould Element, 3% sodium chloride, 3% Abelmoschus Esculentus Linn extract, surplus are water.
(extracting method of Abelmoschus Esculentus Linn extract, the collocation method of seed-soaking liquid and embodiment 1 are completely the same in seed-soaking liquid.)
Wherein, it is also equipped with nutrient solution in step (2) culture cup, the nutrient solution is by following percentage by weight into packet Into：7% manioc waste soak I, 5% trehalose, 3% spirulina extract, 3% stems of dragon fruits extract, surplus For water.
(manioc waste soak I immersion process, the extracting method of spirulina extract, stems of dragon fruits extraction in nutrient solution The extracting method of thing, the collocation method of nutrient solution and embodiment 1 are completely the same.)
Wherein, step (4) branch processing water is made up of the composition of following percentage by weight：7% manioc waste soaking solution II, 5% humic acid, 3% heteroauxin, 3% extract of soybean, surplus are water.
(immersion process of manioc waste soaking solution II, the extracting method of extract of soybean, branch handle water in branch processing water Collocation method and embodiment 1 it is completely the same.)
Wherein, step (5) foliar fertilizer is made up of the composition of following percentage by weight：7% urine, 5% potassium nitrate, 3% sodium dihydrogen phosphate, 3% extract of soybean, surplus are water.
(source of urine, the extracting method of extract of soybean, the collocation method of foliar fertilizer and embodiment 1 are complete in foliar fertilizer It is complete consistent.)
Embodiment 3：
The interplanting method of a kind of wing pod Cassia tora and khuskhus, methods described comprise the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1.5kg/m2Applied amount shed and kill into pre- kind ground Malicious powder, according to 600g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, open ground after covering 25d Film, then apply organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 1.5kg/m2, obtained after fertilising by 17d curing Plant plot；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, by wing pod Cassia tora seed and sterilization Liquid is 1 according to mass ratio:2.5 mixing, are washed after soaking 7min with distilled water, by wing pod Cassia tora seed and seed soaking after washing Liquid is 1 according to mass ratio:3.5 soak 25h in the case where temperature is 27 DEG C of constant temperature, are filtered after immersion, seed is drained Uniformly it is placed on afterwards in culture cup, and is 27 DEG C in temperature, relative humidity 12%, under conditions of intensity of illumination is 900Lx Culture, until growing the high wing pod Cassia tora seedling of 6cm；The mode of washing of the distilled water is：By wing pod Cassia tora seed from sterilization Filter and take out in liquid, be then 1 according to wing pod Cassia tora and distilled water mass ratio:2 mixing, distilled water is discarded after stirring 1min, then It is 1 by wing pod Cassia tora and distilled water mass ratio:2 mixing, stirring, are so washed 5-7 times repeatedly；
(3) wing pod Cassia tora is planted：By the wing pod Cassia tora seedling of step (2) according to 35cm × 35cm seeding row spacing bunch planting in step Suddenly on the plantation plot of (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 25%；
(4) khuskhus branch pre-processes：Clip length is 12cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1-2 Branch, 3 blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch grows 4cm length The khuskhus branch pre-processed is obtained during coring；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 35d, khuskhus branch that step (4) has been pre-processed It is colonized between two adjacent row wing pod Cassia toras, and according to 550g/m2The amount of spraying spray foliar fertilizer into whole plantation plot, It is spaced after 16d according still further to the same amount of spraying sprinkling once.
Wherein, step (1) antivirus powder comprises the following components in parts by weight：20 parts of calcium oxide, the extraction of 10 parts of eucalyptus Thing, 12 parts of Artemisinin, 8 parts of chrysanthemum root bark extract and 6 parts of chitosan.
(the extraction of the extracting method, the extracting method of Artemisinin, chrysanthemum root bark extract of antivirus powder eucalyptus extracts Method, the collocation method for powder of killing virus and embodiment 1 are completely the same.)
Wherein, step (1) organic fertilizer I is comprised the following components in parts by weight：7 parts of vermiculite, 20 parts of manioc waste, 18 parts Mulberry leaf ferment, 20 parts of soybean peel and 30 parts of peanut press pulp.
(manioc waste source, mulberry leaf ferment preparation method, organic fertilizer I production method and embodiment 1 are complete in organic fertilizer I Unanimously.)
Wherein, step (2) thimerosal is made up of the composition of following percentage by weight：0.2% HgCl2, 2% aloe carry It is water to take thing, 3% extract of willow bark, surplus.
(extracting method of aloe extract, the extracting method of extract of willow bark, the collocation method of thimerosal in thimerosal It is completely the same with embodiment 1.)
Wherein, step (2) seed-soaking liquid is made up of the composition of following percentage by weight：2% potassium permanganate, 2% it is red mould Element, 2% sodium chloride, 2% Abelmoschus Esculentus Linn extract, surplus are water.
(extracting method of Abelmoschus Esculentus Linn extract, the collocation method of seed-soaking liquid and embodiment 1 are completely the same in seed-soaking liquid.)
Wherein, it is also equipped with nutrient solution in step (2) culture cup, the nutrient solution is by following percentage by weight into packet Into：6% manioc waste soak I, 4% trehalose, 2% spirulina extract, 2% stems of dragon fruits extract, surplus For water.
(manioc waste soak I immersion process, the extracting method of spirulina extract, stems of dragon fruits extraction in nutrient solution The extracting method of thing, the collocation method of nutrient solution and embodiment 1 are completely the same.)
Wherein, step (4) branch processing water is made up of the composition of following percentage by weight：6% manioc waste soaking solution II, 4% humic acid, 2% heteroauxin, 2% extract of soybean, surplus are water.
(immersion process of manioc waste soaking solution II, the extracting method of extract of soybean, branch handle water in branch processing water Collocation method and embodiment 1 it is completely the same.)
Wherein, step (5) foliar fertilizer is made up of the composition of following percentage by weight：6% urine, 3% potassium nitrate, 2% sodium dihydrogen phosphate, 2% extract of soybean, surplus are water.
(source of urine, the extracting method of extract of soybean, the collocation method of foliar fertilizer and embodiment 1 are complete in foliar fertilizer It is complete consistent.)
Control group 1：
This control group is identical with the implantation methods of embodiment 1, but plot is pre-processed without using antivirus powder, Other methods are identical with embodiment 1.
I.e.：This method comprises the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, backward pre- kind ground in apply organic fertilizer I, organic fertilizer I application Measure as 1kg/m2, plantation plot is obtained by 15d curing after fertilising；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, by wing pod Cassia tora seed and sterilization Liquid is 1 according to mass ratio:2 mixing, are washed after soaking 5min with distilled water, by wing pod Cassia tora seed and seed-soaking liquid after washing It is 1 according to mass ratio:3 soak 24h in the case where temperature is 25 DEG C of constant temperature, are filtered after immersion, after seed is drained Even is placed in culture cup, and is 25 DEG C in temperature, and relative humidity 10%, intensity of illumination is cultivated under conditions of being 800Lx, Until growing the high wing pod Cassia tora seedling of 5cm；The mode of washing of the distilled water is：By wing pod Cassia tora seed from thimerosal mistake Leaching goes out, and is then 1 according to wing pod Cassia tora and distilled water mass ratio:2 mixing, distilled water is discarded after stirring 1min, then by wing pod Cassia is 1 with distilled water mass ratio:2 mixing, stirring, are so washed 5-7 times repeatedly；
(3) wing pod Cassia tora is planted：By the wing pod Cassia tora seedling of step (2) according to 30cm × 30cm seeding row spacing bunch planting in step Suddenly on the plantation plot of (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 20%；
(4) khuskhus branch pre-processes：Clip length is 10cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1 point Branch, 2 blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch grows the root of 2cm length The khuskhus branch pre-processed is obtained during palpus；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 30d, khuskhus branch that step (4) has been pre-processed It is colonized between two adjacent row wing pod Cassia toras, and according to 500g/m2The amount of spraying spray foliar fertilizer into whole plantation plot, It is spaced after 15d according still further to the same amount of spraying sprinkling once.
Control group 2：
This control group is identical with the implantation methods of embodiment 1, but plot is applied fertilizer without using organic fertilizer I, Other methods are identical with embodiment 1.
I.e.：This method comprises the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1kg/m2Applied amount shed antivirus into pre- kind ground Powder, according to 500g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, open ground after covering 20d Film, obtain planting plot；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, by wing pod Cassia tora seed and sterilization Liquid is 1 according to mass ratio:2 mixing, are washed after soaking 5min with distilled water, by wing pod Cassia tora seed and seed-soaking liquid after washing It is 1 according to mass ratio:3 soak 24h in the case where temperature is 25 DEG C of constant temperature, are filtered after immersion, after seed is drained Even is placed in culture cup, and is 25 DEG C in temperature, and relative humidity 10%, intensity of illumination is cultivated under conditions of being 800Lx, Until growing the high wing pod Cassia tora seedling of 5cm；The mode of washing of the distilled water is：By wing pod Cassia tora seed from thimerosal mistake Leaching goes out, and is then 1 according to wing pod Cassia tora and distilled water mass ratio:2 mixing, distilled water is discarded after stirring 1min, then by wing pod Cassia is 1 with distilled water mass ratio:2 mixing, stirring, are so washed 5-7 times repeatedly；
(3) wing pod Cassia tora is planted：By the wing pod Cassia tora seedling of step (2) according to 30cm × 30cm seeding row spacing bunch planting in step Suddenly on the plantation plot of (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 20%；
(4) khuskhus branch pre-processes：Clip length is 10cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1 point Branch, 2 blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch grows the root of 2cm length The khuskhus branch pre-processed is obtained during palpus；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 30d, khuskhus branch that step (4) has been pre-processed It is colonized between two adjacent row wing pod Cassia toras, and according to 500g/m2The amount of spraying spray foliar fertilizer into whole plantation plot, It is spaced after 15d according still further to the same amount of spraying sprinkling once.
Control group 3：
This control group is identical with the implantation methods of embodiment 1, but wing pod Cassia tora seed is not pre-processed, its Its method is identical with embodiment 1.
I.e.：This method comprises the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1kg/m2Applied amount shed antivirus into pre- kind ground Powder, according to 500g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, open ground after covering 20d Film, then apply organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 1kg/m2, planted after fertilising by 15d curing Plant plot；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, wing pod Cassia tora seed is planted in In sand ground, until growing the high wing pod Cassia tora seedling of 5cm；
(3) wing pod Cassia tora is planted：By the wing pod Cassia tora seedling of step (2) according to 30cm × 30cm seeding row spacing bunch planting in step Suddenly on the plantation plot of (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 20%；
(4) khuskhus branch pre-processes：Clip length is 10cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1 point Branch, 2 blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch grows the root of 2cm length The khuskhus branch pre-processed is obtained during palpus；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 30d, khuskhus branch that step (4) has been pre-processed It is colonized between two adjacent row wing pod Cassia toras, and according to 500g/m2The amount of spraying spray foliar fertilizer into whole plantation plot, It is spaced after 15d according still further to the same amount of spraying sprinkling once.
Control group 4：
This control group is identical with the implantation methods of embodiment 1, but khuskhus is not pre-processed, other methods It is identical with embodiment 1.
I.e.：This method comprises the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1kg/m2Applied amount shed antivirus into pre- kind ground Powder, according to 500g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, open ground after covering 20d Film, then apply organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 1kg/m2, planted after fertilising by 15d curing Plant plot；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, by wing pod Cassia tora seed and sterilization Liquid is 1 according to mass ratio:2 mixing, are washed after soaking 5min with distilled water, by wing pod Cassia tora seed and seed-soaking liquid after washing It is 1 according to mass ratio:3 soak 24h in the case where temperature is 25 DEG C of constant temperature, are filtered after immersion, after seed is drained Even is placed in culture cup, and is 25 DEG C in temperature, and relative humidity 10%, intensity of illumination is cultivated under conditions of being 800Lx, Until growing the high wing pod Cassia tora seedling of 5cm；The mode of washing of the distilled water is：By wing pod Cassia tora seed from thimerosal mistake Leaching goes out, and is then 1 according to wing pod Cassia tora and distilled water mass ratio:2 mixing, distilled water is discarded after stirring 1min, then by wing pod Cassia is 1 with distilled water mass ratio:2 mixing, stirring, are so washed 5-7 times repeatedly；；
(3) wing pod Cassia tora is planted：By the wing pod Cassia tora seedling of step (2) according to 30cm × 30cm seeding row spacing bunch planting in step Suddenly on the plantation plot of (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 20%；
(4) khuskhus branch is handled：Clip length is 10cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1 point Branch, 2 blades obtain khuskhus branch；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 30d, khuskhus branch that step (4) has been pre-processed It is colonized between two adjacent row wing pod Cassia toras, and according to 500g/m2The amount of spraying spray foliar fertilizer into whole plantation plot, It is spaced after 15d according still further to the same amount of spraying sprinkling once.
Control group 5：
This control group is identical with the implantation methods of embodiment 1, but only plants wing pod Cassia tora, and other methods are with implementing Example 1 is identical.
I.e.：This method comprises the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1kg/m2Applied amount shed antivirus into pre- kind ground Powder, according to 500g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, open ground after covering 20d Film, then apply organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 1kg/m2, planted after fertilising by 15d curing Plant plot；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, by wing pod Cassia tora seed and sterilization Liquid is 1 according to mass ratio:2 mixing, are washed after soaking 5min with distilled water, by wing pod Cassia tora seed and seed-soaking liquid after washing It is 1 according to mass ratio:3 soak 24h in the case where temperature is 25 DEG C of constant temperature, are filtered after immersion, after seed is drained Even is placed in culture cup, and is 25 DEG C in temperature, and relative humidity 10%, intensity of illumination is cultivated under conditions of being 800Lx, Until growing the high wing pod Cassia tora seedling of 5cm；The mode of washing of the distilled water is：By wing pod Cassia tora seed from thimerosal mistake Leaching goes out, and is then 1 according to wing pod Cassia tora and distilled water mass ratio:2 mixing, distilled water is discarded after stirring 1min, then by wing pod Cassia is 1 with distilled water mass ratio:2 mixing, stirring, are so washed 5-7 times repeatedly；；
(3) wing pod Cassia tora is planted：By the wing pod Cassia tora seedling of step (2) according to 30cm × 30cm seeding row spacing bunch planting in step Suddenly on the plantation plot of (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 20%
Control group 6：
This control group is identical with the implantation methods of embodiment 1, but only plants khuskhus, other methods and embodiment 1 It is identical.
I.e.：This method comprises the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1kg/m2Applied amount shed antivirus into pre- kind ground Powder, according to 500g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, open ground after covering 20d Film, then apply organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 1kg/m2, planted after fertilising by 15d curing Plant plot；
(2) khuskhus branch pre-processes：Clip length is 10cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1 point Branch, 2 blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch grows the root of 2cm length The khuskhus branch pre-processed is obtained during palpus；
(3) khuskhus are interplanted：Khuskhus are planted according to 20cm × 20cm seeding row spacing, and according to 500g/m2's The amount of spraying sprays foliar fertilizer into whole plantation plot, is spaced after 15d according still further to the same amount of spraying sprinkling once.
Testing experiment 1：
Experiment is completed in the Experimental Base of this research institute, experiment plot is artificially fabricated to rich cadmium (Cd) environment cadmium content and reached 27.89mg/kg, planted according to embodiment 1-3 and control group 1-6 method, after planting 1 year, uniformly scrape surface impurity Layer, test 2cm thickness soil horizon, 5cm thickness soil horizon, the nitrogen of 10cm thickness soil horizon and 20cm thickness soil horizons, phosphorus, cadmium (Cd) content and The content of organic matter, specifically it is shown in Table 1：
Table 1
As seen from the above table, the thick soil of embodiment 1-3 2cm thickness soil horizon, 5cm thickness soil horizon, 10cm thickness soil horizon and 20cm Nitrogen, phosphorus and the content of organic matter of earth layer are above control group 1-6, and in control group 1-6, control group 5, the thick soil of the 2cm of control group 6 Earth layer, 5cm thickness soil horizon, the nitrogen of 10cm thickness soil horizon and 20cm thickness soil horizons, phosphorus and content of organics are minimum, illustrate this Shen Please used in khuskhus and wing pod Cassia tora interplanting method, planting khuskhus or wing pod Cassia tora than single has stronger fixed nitrogen energy Power, it can also significantly improve phosphorus and the content of organic matter in soil；And embodiment 1-3 2cm thickness soil horizon, 5cm thickness soil horizon, 10cm Cadmium (Cd) content of thick soil horizon and 20cm thickness soil horizon is significantly lower than control group 1-6, and in control group 1-6, control group 5, right According to the 2cm thickness soil horizon of group 6,5cm thickness soil horizon, 10cm thickness soil horizon and 20cm thickness soil horizons cadmium (Cd) Content most Height, illustrate khuskhus used in this application and wing pod Cassia tora interplanting method, planting khuskhus or wing pod Cassia tora than single has more The purpose of cadmium (Cd) content in strong reduction soil.
Testing experiment 2：
Experiment is completed in the Experimental Base of this research institute, is planted according to embodiment 1-3 and control group 1-6 method, After khuskhus plant 10d, each processing randomly selects 3 plants of seedling, determines the children of wing pod Cassia tora and khuskhus respectively with ruler Seedling height of seedling.Concrete condition is shown in Table 2：
Table 2
As seen from the above table：Embodiment 1-3 seedling illustrates antivirus powder, the organic fertilizer of the application apparently higher than control group 1-6 I, wing pod Cassia tora Seeds preprocess, khuskhus are pre-processed, the method for wing pod Cassia tora and khuskhus interplanting can effectively improve post Flowers and plants and the growth ability of wing pod Cassia tora.
Testing experiment 3：
Single factor experiment is carried out to wing pod Cassia tora Seeds preprocess：Wing pod Cassia tora seed is divided into 9 groups, according to such as table 3 processing mode is handled：
Table 3
After wing pod Cassia tora seed is handled according to test group 1-9 processing method, in the bar that intensity of illumination is 800Lx 7d is cultivated under part, daily from after planting 1d regularly to count day germination number, after planting 7d terminates to germinate, and calculates germinating energy, germination Rate, each processing randomly select 3 plants of seedling, determine seedling height of seedling with ruler, main root is grown, statistics lateral root number.Concrete condition is shown in Table 4：
Table 4
Group Germinating energy (%) Germination percentage (%) Seedling height of seedling (cm) Main root grows (cm) Lateral root number (bar)
Test group 1 41.67 31.67 8.73 7.86 15.31
Test group 2 41.98 31.98 8.87 8.11 15.48
Test group 3 46.67 31.25 9.20 9.16 15.67
Test group 4 47.98 32.56 10.34 9.31 15.94
Test group 5 48.29 32.87 9.34 9.56 16.11
Test group 6 39.14 23.33 8.49 9.21 16.00
Test group 7 43.30 33.20 8.26 8.63 15.16
Test group 8 44.14 34.17 9.23 9.04 15.50
Test group 9 44.98 35.14 9.84 8.52 15.82
Control group 53.26 61.02 13.02 10.21 17.03
As seen from the above table, test group 1-9 germinating energy, germination percentage, seedling height of seedling, main root be long and radical all significantly lower than pair According to group, illustrate that thimerosal used in this application, seed-soaking liquid, culture liquid energy significantly improve the germinating energy of wing pod Cassia tora seedling, germination Rate, seedling height of seedling, main root length and radical；Main active is wherein played in thimerosal is aloe extract and willow bark extraction Thing；Main active is played in seed-soaking liquid is Abelmoschus Esculentus Linn extract；Main active is played in nutrient solution is manioc waste leaching Steep liquid I, spirulina extract and stems of dragon fruits extract.
In summary, interplanted using the method for the present invention using wing pod Cassia tora and khuskhus, raising can be effectively played Nitrogen, phosphorus and the content of organic matter in soil, improve soil environment, while can also effectively reduce cadmium content and play the work for improving soil With, during interplanting, a series of used processing methods：Antivirus powder, organic fertilizer I, wing pod Cassia tora Seeds preprocess and post flower Grass, which carries out pretreatment, can be effectively improved the germination percentage of wing pod Cassia tora seed, accelerate the growth of wing pod Cassia tora and khuskhus.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims (8)

1. the interplanting method of a kind of wing pod Cassia tora and khuskhus, it is characterised in that methods described comprises the following steps：
(1) readjustment of field plot：Pre- kind is ploughed, afterwards according to 1kg/m2-2kg/m2Applied amount shed and kill into pre- kind ground Malicious powder, according to 500g/m2-800g/m2Irrigation amount watered into pre- kind ground, then cover mulch film into pre- kind ground, cover 20d- Mulch film is opened after 30d, then applies organic fertilizer I into pre- kind ground, organic fertilizer I applied amount is 1kg/m2-2kg/m2, passed through after fertilising The curing for crossing 15d-20d obtains planting plot；
(2) wing pod Cassia tora Seeds preprocess：The full wing pod Cassia tora seed of health is selected, wing pod Cassia tora seed is pressed with thimerosal It is 1 according to mass ratio:2-3 is mixed, and is washed after soaking 5min-10min with distilled water, by wing pod Cassia tora seed and leaching after washing Kind liquid is 1 according to mass ratio:3-4 soaks 24h-26h in the case where temperature is 25 DEG C -30 DEG C of constant temperature, is filtered after immersion, Uniformly it is placed on after seed is drained in culture cup, and is 25 DEG C -30 DEG C, relative humidity 10%-15% in temperature, illumination Intensity is cultivated under conditions of being 800Lx-1000Lx, until growing the high wing pod Cassia tora seedling of 5cm-7cm；The distilled water is washed The mode of washing is：Wing pod Cassia tora seed is filtered to taking-up from thimerosal, is then 1 according to wing pod Cassia tora and distilled water mass ratio:2 Mixing, distilled water is discarded after stirring 1min, then by wing pod Cassia tora and distilled water mass ratio be 1:2 mixing, stirring, are so washed repeatedly Wash 5-7 times；
(3) wing pod Cassia tora is planted：Seeding row spacing cave by the wing pod Cassia tora seedling of step (2) according to 30cm-40cm × 30cm-40cm Broadcast on the plantation plot of step (1), and irregularly to the watering of plantation plot, the relative humidity for keeping plantation plot is 20%- 30%；
(4) khuskhus branch pre-processes：Clip length is 10cm-15cm khuskhus branch, cuts off unnecessary branches and leaves, only stays 1-2 Individual branch, 2-3 piece blades；Then it will be soaked in the bottom insertion branch processing water of khuskhus branch, treat that branch is grown The khuskhus branch pre-processed is obtained during the coring of 2cm-5cm length；
(5) khuskhus are interplanted：After step (3) plantation wing pod Cassia tora 30d-40d, khuskhus branch that step (4) has been pre-processed It is colonized between two adjacent row wing pod Cassia toras, and according to 500g/m2-600g/m2The amount of spraying sprayed into whole plantation plot Foliar fertilizer, it is spaced after 15d-17d according still further to the same amount of spraying sprinkling once.
A kind of 2. interplanting method of wing pod Cassia tora and khuskhus according to claim 1, it is characterised in that the step (1) Antivirus powder comprises the following components in parts by weight：15 parts -25 parts of calcium oxide, 8 parts -13 parts of eucalyptus extracts, 9 parts -15 parts Artemisinin, 7 parts -13 parts of chrysanthemum root bark extract and 5 parts -11 parts of chitosan.
A kind of 3. interplanting method of wing pod Cassia tora and khuskhus according to claim 1, it is characterised in that the step (1) Organic fertilizer I is comprised the following components in parts by weight：5 parts -10 parts of vermiculite, 15 parts -25 parts of manioc waste, 13 parts -23 parts of mulberry Leaf ferment, 14 parts -26 parts of soybean peel and 23 parts -35 parts of peanut press pulp.
A kind of 4. interplanting method of wing pod Cassia tora and khuskhus according to claim 1, it is characterised in that the step (2) Thimerosal is made up of the composition of following percentage by weight：0.1%-0.3% HgCl2, 1%-3% aloe extract, 2%- 5% extract of willow bark, surplus are water.
A kind of 5. interplanting method of wing pod Cassia tora and khuskhus according to claim 1, it is characterised in that the step (2) Seed-soaking liquid is made up of the composition of following percentage by weight：1%-3% potassium permanganate, 1%-3% gibberellin, 1%-3% Sodium chloride, 1%-3% Abelmoschus Esculentus Linn extract, surplus are water.
A kind of 6. interplanting method of wing pod Cassia tora and khuskhus according to claim 1, it is characterised in that the step (2) Nutrient solution is also equipped with culture cup, the nutrient solution is made up of the composition of following percentage by weight：5%-7% manioc waste immersion Liquid I, 2%-5% trehalose, 1%-3% spirulina extract, 1%-3% stems of dragon fruits extract, surplus are water.
A kind of 7. interplanting method of wing pod Cassia tora and khuskhus according to claim 1, it is characterised in that the step (4) Branch processing water is made up of the composition of following percentage by weight：5%-7% manioc waste soaking solution II, 2%-5% humic acid, 1%-3% heteroauxin, 1%-3% extract of soybean, surplus are water.
A kind of 8. interplanting method of wing pod Cassia tora and khuskhus according to claim 1, it is characterised in that the step (5) Foliar fertilizer is made up of the composition of following percentage by weight：5%-7% urine, 2%-5% potassium nitrate, 1%-3% phosphoric acid Sodium dihydrogen, 1%-3% extract of soybean, surplus are water.
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CN108966985A (en) * 2018-05-31 2018-12-11 广西浪伏茶业股份有限公司 A kind of method of Ling Yaoxing and plum interplanting
CN109749753A (en) * 2019-01-25 2019-05-14 甘肃省农业科学院旱地农业研究所 soil conditioner and preparation method thereof
CN109749753B (en) * 2019-01-25 2022-09-20 甘肃省农业科学院旱地农业研究所 Soil conditioner and preparation method thereof

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