CN104782684A - Tree wound protective agent, and preparation and application methods thereof - Google Patents

Tree wound protective agent, and preparation and application methods thereof Download PDF

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Publication number
CN104782684A
CN104782684A CN201510215792.9A CN201510215792A CN104782684A CN 104782684 A CN104782684 A CN 104782684A CN 201510215792 A CN201510215792 A CN 201510215792A CN 104782684 A CN104782684 A CN 104782684A
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protective agent
trees
water
wound
parts
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CN104782684B (en
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何素芬
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Sichuan Yunran Landscape Engineering Co ltd
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YIBIN YUNCHEN ARBOR GARDEN CO Ltd
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Abstract

The invention relates to the field of garden tree protection and restoration, particularly a protective agent for restoring damaged tree wounds, and preparation and application thereof. The protective agent is composed of the following components in parts by weight: 9-11 parts of sulfur colloidal suspension agent, 9-11 parts of trimethylglycine trimethylglycocoll, 0.4-0.6 part of polychlorobenzoic acid, 10-20 parts of alcohol, 0.15-0.25 part of high fat film emulsion, 0.1-0.2 part of bactericide and 900-1100 parts of pepper water. The protective agent can perform long-term disinfecting, sterilizing and antiseptic functions on tree wounds and rots, holes due to insects or rotting, and cracking or shedding at the bark damage, accelerate the formation of callus, prevent the secondary infection of pathogenic bacteria and insects, promote the quick healing of saw bites and unhealed annular cuts and prevent diseases and insects from infection.

Description

A kind of trees Wound protective agent and preparation thereof, using method
Technical field
The present invention relates to ornamental trees and shrubs Conservative restoration field, being specifically related to a kind of for damaging protectant that trees wound repairs and preparation thereof, use.
Background technology
In trees daily management, wound, bark breakage easily make viral germ or insect pest invade people and disease occurs, rainwater drip washing makes again wound that wooden rotten, bad skin generation physiological disturbance occurs, light then affect tree grafting growth and survive and grow, heavy then cause tree death, bring economic loss to operator.Only at a cm in the speed 1 year of pruning (saw after) wound general edge skin, the wound of branch small size, ability healing in a year.Therefore, the Wound protection process of trees after repairing, cutting or after trunk broken skin is the very important technical measures of in cultivation and management of tree one.
But at present, various wound healing agent for plants, healing ointment etc. that market is sold, not only commodity price is high, uses rear wound healing and sterilizing disease-preventing poor effect, and even not having of having promotes wound healing and sterilization.
And current plants wound protectant, consolidant, healing ointment etc. on the market; often declare to hold time at about 7 ~ 30 days after smearing; but it is general 7 days ~ about 10 days time; good protection, healing effect cannot be maintained; bacterium for killing insect effect declines; wound is easily dry and cracked, and trees cannot be healed completely.
In addition, cause trunk to rot comparatively seriously for sick worm harm and produce the trees position of hole, because insect pest is eaten into xylem and is caused on trunk and have hole darker, although execute insecticide to control to kill insect, but because worm channel bacteria infection causes bark and xylem partial decomposition, rot to infect area comparatively large, again there is hole simultaneously, easily dry and cracked, aforementioned various wound healing agent for plants on the market, healing ointment can not play a good healing effect to this situation
In sum, the products such as now known trees Wound protective agent, for trees because of insect pest, rot, prune the process such as wound and cannot reach a more complete good protected effect.
If a kind of safe and efficient and practical trees Wound protective agent can be developed; can for because of insect pest, rot, prune all kinds of situation such as wound and protect Promotive union preferably; simultaneously can play protection healing effect equally for the larger rotten region of trees and/or more worm hole situation; energy acute activation plant immune system; promote cell division and growth metabolism; suppress germ hereby raw procreation; rehabilitation plant limb thickness wound; accelerating wound healing; to solve woods fruit tree wound healing problem, the cultivation of garden fruit tree wood will be very beneficial for.
Summary of the invention
The object of the present invention is to provide a kind of trees Wound protective agent and preparation thereof, using method, to solve the problem in above-mentioned background technology.
For realizing the object of the invention, the technical scheme of employing is:
A kind of trees Wound protective agent, it is characterized in that: count by weight, be made up of following component: sulphur colloidal suspension agent 9 ~ 11 parts, trimethylglycine 9 ~ 11 parts, benzac 0.4 ~ 0.6 part, alcohol 10 ~ 20 parts, high fat film emulsion 0.15 ~ 0.25 part, 0.1 ~ 0.2 part, bactericide, pepper water 900 ~ 1100 parts;
Described pepper water is counted by weight, is made up of following component: 95 ~ 105 parts, water, 4 ~ 6 parts, Chinese prickly ash.
Further, described bactericide comprises the one in octicin solution Fluoxastrobin, thiophanate methyl, tpn, copper rosinate.
Further, the preparation method of described a kind of trees Wound protective agent is as follows:
A. pepper water preparation: count by weight, water intaking, Chinese prickly ash, pouring into water in pot, put into Chinese prickly ash, boiling to going out pepper flavor after boiled, when in pot, water is left 45% ~ 55%, filters after discarding residue cooling for subsequent use;
B. by bactericide, dissolve for subsequent use with Part I pepper water;
C. by trimethylglycine, dissolve for subsequent use with Part II pepper water;
D. by 50% sulphur colloidal suspension agent, dissolve for subsequent use with Part III pepper water;
E. by benzac, for subsequent use after dissolving with alcohol;
F. 27% high fat film emulsion Part IV pepper water is diluted;
G. mix: pour bactericide for subsequent use, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film into container successively, add surplus pepper water constant volume, fully whisk mixing, make solution become viscoloid shape, to obtain final product.
The using method of a kind of trees Wound protective agent as described above, is characterized in that: spread upon trees wound, rotten place, because of the hole place that insect pest or rot causes, and bark damage cracking or the place that comes off.
Further, described in spread upon trees wound and be specially band girdle mouth trees newly cut, saws wound, do not heal, get 20 ~ 100ml Wound protective agent with the hairbrush libation at an ancient wedding ceremony, even spread plants wound.
Further; the described rotten place that spreads upon is specially woody plant trunk rot, dry rot, ring spot cutter dark standardized long circle around sick scar; intersect at middle part and draw number cutter; deeply reach health tissues or xylem; get 50 ~ 300ml Wound protective agent with the hairbrush libation at an ancient wedding ceremony again and smear disease scar, every 18 ~ 22 days once, after continuous 2 times, check whether downright bad sick scar comes off; whether new organization grows, and according to circumstances smears again.
Further; described spreading upon causes trunk rot comparatively serious and produce the position of hole because insect pest or the hole place of rotting to cause are specially for sick worm harm; the wooden slag that first rotted in hole portion is wiped off; after rinsing with clear water; get 50 ~ 500ml Wound protective agent with the hairbrush libation at an ancient wedding ceremony again and smear whole sick portion and hole, after 15 days, carry out single treatment again.
Further; described bark damage cracking or the place that comes off of spreading upon is specially bark cracking or bark local shedding; expose the ancient tree of xylem; first the bark of dry rot is cut away along exposed xylem edge with cutter; carve the xylem of hindering exposed part again; get 50 ~ 500ml Wound protective agent with the hairbrush libation at an ancient wedding ceremony to smear, once smear again after 28 ~ 30 days.
Principle of the present invention is as follows:
Sulphur in the sulphur colloidal suspension agent related in the present invention is a kind of ancient desinsection, bactericide, inexpensive, and from non-resistant, lasting over over thousands of year.Sulphur colloidal suspension agent is a kind of novel protective agent in inorganic sulfur bactericide, all has good control efficiency, have concurrently and have killing action to bacterium, worm multiple germ and the damage by disease and insect such as worm's ovum, mite class.Its Main Function mechanism is the formation by spindle in interference germ mitosis process, thus affects germ cell division, reaches suppression and killing pathogenic bacteria.Sulphur colloidal suspension agent fine size, suspensibility are high, cohesiveness is strong, have good dispersion, adhesivity and anti-rain drop erosion.
High fat film: be a kind of pure white, fine and smooth cloudy aqueous material, use the fatty sorrel that rear plant corpus surface has one deck very thin, germ controls because there being film, can not expand or seldom expand to cause harm after invaded plants body, thus reaches diseases prevention object.High fat film has good control efficiency to most of fungal disease, and its diseases prevention is physical action, and germ can not develop immunity to drugs, and itself is nontoxic, harmless, nontoxic to people, plant, fish.
The preferred octicin solution of bactericide of the present invention: be a kind of amino acids systemic fungicide, active ingredient is glycine substitutive derivative, there is the features such as efficient, low toxicity, noresidue, and have good permeability, there is certain eradicant action to the germ that hides invaded in bark, the disease that multiple fungi, bacterium and virus cause can be prevented and treated.Sterilization mechanism solidifies germ protein, suppresses mycelia and the spore growth of germ, destroy germ cell membrane, suppresses germ to breathe, make the sex change of germ enzyme system, makes enzyme denaturation and play antibacterial and bactericidal action, reaching and kill germ object.
Trimethylglycine (amino acid humectant): be a kind of natural structure material amphoteric ion type moisturizing ingredient, extremely soluble in water, energy activated cell vigor, improves trees body surface moisture holding capacity, keeps moistening and prevent drying.Very heat-resisting, acidproof and alkaline-resisting, and the scope of application is wide, easy to operate and safety and stability.
Benzac: be a kind of basic element of cell division, can promote that cell division is broken up, and delays the degraded of plant corpus internal protein, break plant tip advantage, promote collateral generation, delaying senility, extend plant life, its activity is all high than 6-benzyl aminopurine, cytokinin.Plant easily absorbs, and can not transport in plant corpus rapidly again, and after process plants wound, favorable incentive local cells divides, accelerating wound effect.
Pepper water: have the effect such as sterilization, sterilization, as the solvent of wound healing protective agent, can strengthen and improve protectant disinfection, disinsection efficiency.
Applicant surprisingly finds, sulphur colloidal suspension agent and high fat film emulsion, trimethylglycine at pepper water as solvent, when using together, there is outstanding effect, there is splendid dissolubility in pepper water, and effectively can extend the action effective of sulphur colloidal suspension agent, and under the effect of high fat film emulsion, under bark damage cracking or the place that comes off can be in the moistening effect of trimethylglycine for a long time, effectively prevent bark dry and cracked further.
In trees wound; rotten place; because of in the situations such as the hole place that insect pest or rot causes; bark there is certain damage cracking or have and come off more or less around; utilize the present invention can at protection wound, while Promotive union; around bark can not further develop cracking, avoids germ, insect pest etc. and enters from other approach and cause damage.
Wound first have employed trimethylglycine (amino acid humectant) and is added in trees Wound protective agent as humectant composition, ftracture at bark, come off, and there is the dark wound place of a sizing, can the certain humidity of long-acting maintenance, and under the fatty sorrel of high fat film component formation, cooperatively interact with fatty sorrel, both the fatty sorrel duration had been extended, the membrane permeability of fatty sorrel can not be affected again, fat sorrel is not easy in a short time (1 ~ 7 day) forfeiture effect, within mid-term, (7 ~ 15 days) are air-dry gradually comparatively slowly, simultaneously under moisture retention effect benzac and bactericide to be not easy because of fatty sorrel in 7 ~ 15 days dry and cracked, the reasons such as weathering, forfeiture effect gradually, effectively extend the acting duration of Wound protective agent of the present invention.
Tool of the present invention has the following advantages:
One, the present invention is to trees wound, rotten place, because of the hole place that insect pest or rot causes, the effect of long-acting disinfection sterilization and anticorrosion can be played by bark damage cracking or the place that comes off, accelerate Callus formation, preventing the superinfection of germ insect pest, promoting that cutting saw kerf, the band girdle mouth that do not heal can, with closing, prevent disease worm from contaminating rapidly.
Two, the present invention can form diaphragm rapidly at plant otch after smearing, and has the membrane permeability of soil moisture retention, both prevents moisture, the loss of nutrient, tree body of not burning again.
Three, the present invention effectively promotes the regeneration capacity of callus, impels trees damage location quickly-healing.
Four, the present invention is viscoloid shape, and conveniently spread upon arbitrary position of trees, cost is lower, and preparation method is simple, is suitable for promoting.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment 1:
A kind of trees Wound protective agent:
A. pepper water preparation: count by weight, getting 95 parts of water, 4 portions of Chinese prickly ashes, poured into by water in pot, put into Chinese prickly ash, boiling to going out pepper flavor after boiled, when water is left 45% in pot, filters after discarding residue cooling for subsequent use, makes the above-mentioned pepper water of 900g;
B. by 0.1g octicin solution, dissolve for subsequent use with 100g pepper water;
C. by 9g trimethylglycine, dissolve for subsequent use with 100g pepper water;
D. by 50% sulphur colloidal suspension agent of 9g, dissolve for subsequent use with 100g pepper water;
E. by 0.4g benzac, for subsequent use after dissolving with 10g alcohol;
F. the 27% high fat film emulsion 100g pepper water of 0.15g is diluted;
G. mix: pour octicin solution for subsequent use, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film into container successively, add surplus 500g pepper water constant volume, fully whisk mixing, make solution become viscoloid shape, to obtain final product.
The fragrant camphor tree of new transplanting; rice 45 ~ 50 centimeters, footpath; in order to reduce leaf transpiration dehydration during transplanting; only leave trees skeleton; remaining branches and leaves is all cut and is amputated; get the present invention with the libation at an ancient wedding ceremony and smear protection to trees overground part sub-cut saw wound, each wound smears 20 ~ 50ml the present invention, until new branches and leaves germination and growth has had no and cut saw wound and occur that obfuscation is gone mouldy rotten.
Embodiment 2:
A kind of trees Wound protective agent:
A. pepper water preparation: count by weight, getting 105 parts of water, 6 portions of Chinese prickly ashes, poured into by water in pot, put into Chinese prickly ash, boiling to going out pepper flavor after boiled, when water is left 55% in pot, filters after discarding residue cooling for subsequent use, makes the above-mentioned pepper water of 1100g;
B. by 0.2g octicin solution, dissolve for subsequent use with 100g pepper water;
C. by 11g trimethylglycine, dissolve for subsequent use with 100g pepper water;
D. by 50% sulphur colloidal suspension agent of 11g, dissolve for subsequent use with 100g pepper water;
E. by 0.6g benzac, for subsequent use after dissolving with 20g alcohol;
F. the 27% high fat film emulsion 100g pepper water of 0.25g is diluted;
G. mix: pour octicin solution for subsequent use, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film into container successively, add surplus 700g pepper water constant volume, fully whisk mixing, make solution become viscoloid shape, to obtain final product.
For the rot of perennation trees; with cutter dark standardized circle around sick scar; intersect at middle part and draw number cutter, deeply reach health tissues or xylem, get protectant 50 ~ 100ml of the present invention with the hairbrush libation at an ancient wedding ceremony and be applied in disease scar position; smeared once every 20 days; check downright bad sick scar after continuous 2 times, major part comes off, grows new organization; the bad local area of healing is once brushed again, almost reaches complete.
Embodiment 3:
A kind of trees Wound protective agent:
A. pepper water preparation: count by weight, getting 100 parts of water, 5 portions of Chinese prickly ashes, poured into by water in pot, put into Chinese prickly ash, boiling to going out pepper flavor after boiled, when water is left 50% in pot, filters after discarding residue cooling for subsequent use, makes the above-mentioned pepper water of 1000g;
B. by 0.15g Fluoxastrobin, dissolve for subsequent use with 100g pepper water;
C. by 10g trimethylglycine, dissolve for subsequent use with 100g pepper water;
D. by 50% sulphur colloidal suspension agent of 10g, dissolve for subsequent use with 100g pepper water;
E. by 0.5g benzac, for subsequent use after dissolving with 15g alcohol;
F. the 27% high fat film emulsion 100g pepper water of 0.2g is diluted;
G. mix: pour Fluoxastrobin for subsequent use, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film into container successively, add surplus 600g pepper water constant volume, fully whisk mixing, make solution become viscoloid shape, to obtain final product.
Ammonium alum method Shu Age 200 years, 1.5 meters, trees rice footpath, on the trunk of distance 50 centimeters, ground, cause on trunk have hole because old carpenter worm eats into xylem, execute insecticide control kill insect, but due to worm channel bacteria infection cause bark and xylem partial decomposition wide 50 centimeters, deeply reach 15 ~ 25 centimeters.After checking position of rotting, rotten position is processed, first remove and wipe rotten wooden slag off until health tissues; after clear water is rinsed well; get protectant 100 ~ 300ml of the present invention with the hairbrush libation at an ancient wedding ceremony carefully to smear, check after 15 days, rotten symptom is controlled; 2 ~ 3 process are carried out again continuously; every minor tick 20 days, after healing substantially in empty position, fills up hole after concocting with yellow mud bar with pulverized limestone; by trunk former state, improve and view and admire and commodity value.
Embodiment 4:
A kind of trees Wound protective agent:
A. pepper water preparation: count by weight, getting 100 parts of water, 5 portions of Chinese prickly ashes, poured into by water in pot, put into Chinese prickly ash, boiling to going out pepper flavor after boiled, when water is left 50% in pot, filters after discarding residue cooling for subsequent use, makes the above-mentioned pepper water of 1000g;
B. by 0.15g tpn, dissolve for subsequent use with 100g pepper water;
C. by 10g trimethylglycine, dissolve for subsequent use with 100g pepper water;
D. by 50% sulphur colloidal suspension agent of 10g, dissolve for subsequent use with 100g pepper water;
E. by 0.5g benzac, for subsequent use after dissolving with 15g alcohol;
F. the 27% high fat film emulsion 100g pepper water of 0.2g is diluted;
G. mix: pour tpn for subsequent use, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film into container successively, add surplus 600g pepper water constant volume, fully whisk mixing, make solution become viscoloid shape, to obtain final product.
Ginkgo 12 strain Shu Age is had all in 70 ~ 85 years in certain university campus, 70 ~ 80 centimeters, trunk rice footpath, due to human factor (all the year round teachers and students take exercise and pat trees, nail, pulling cord airing clothes etc.), cause trunk apart from 1.2 ~ 1.5 meters, ground eminence bark local shedding, Naked exposes xylem, has both affected trunk attractive in appearance, more affect arboreal growth.For this symptom; instruct afforestation design workman; bark with cutter along exposed xylem edge by the withered dry rot that sustains damage cuts away; firmly hinder exposed part quarter again and deeply reach health tissues or xylem; get protectant 200 ~ 300ml of the present invention with the hairbrush libation at an ancient wedding ceremony and be applied in damaged part, within 30 days, smear again once, after double; the bark revealing xylem edge along Naked just defines callus, and inwardly expands gradually.Hang up warning sign, be combined in the students and faculty the education carrying out love trees, famous and precious trees are effectively protected.
Below that the present invention contrasts common trees Wound protective agent effect table on the market:
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (8)

1. a trees Wound protective agent, it is characterized in that: count by weight, be made up of following component: sulphur colloidal suspension agent 9 ~ 11 parts, trimethylglycine 9 ~ 11 parts, benzac 0.4 ~ 0.6 part, alcohol 10 ~ 20 parts, high fat film emulsion 0.15 ~ 0.25 part, 0.1 ~ 0.2 part, bactericide, pepper water 900 ~ 1100 parts;
Described pepper water is counted by weight, is made up of following component: 95 ~ 105 parts, water, 4 ~ 6 parts, Chinese prickly ash.
2. a kind of trees Wound protective agent according to claim 1, is characterized in that: described bactericide comprises the one in octicin solution, Fluoxastrobin, thiophanate methyl, tpn, copper rosinate.
3. a kind of trees Wound protective agent according to claim 1, is characterized in that: the preparation method of described a kind of trees Wound protective agent is as follows:
A. pepper water preparation: count by weight, water intaking, Chinese prickly ash, pouring into water in pot, put into Chinese prickly ash, boiling to going out pepper flavor after boiled, when in pot, water is left 45% ~ 55%, filters after discarding residue cooling for subsequent use;
B. by bactericide, dissolve for subsequent use with Part I pepper water;
C. by trimethylglycine, dissolve for subsequent use with Part II pepper water;
D. by 50% sulphur colloidal suspension agent, dissolve for subsequent use with Part III pepper water;
E. by benzac, for subsequent use after dissolving with alcohol;
F. 27% high fat film emulsion Part IV pepper water is diluted;
G. mix: pour bactericide for subsequent use, trimethylglycine, sulphur colloidal suspension agent, benzac, high fat film into container successively, add surplus pepper water constant volume, fully whisk mixing, make solution become viscoloid shape, to obtain final product.
4. the using method of a kind of trees Wound protective agent as described in any one of claim 1 ~ 3, is characterized in that: spread upon trees wound, rotten place, because of the hole place that insect pest or rot causes, and bark damage cracking or the place that comes off.
5. the using method of a kind of trees Wound protective agent according to claim 4; it is characterized in that: described in spread upon trees wound and be specially band girdle mouth trees newly cut, saws wound, do not heal; 20 ~ 100ml Wound protective agent is got, even spread plants wound with the hairbrush libation at an ancient wedding ceremony.
6. the using method of a kind of trees Wound protective agent according to claim 4; it is characterized in that: described in spread upon rotten place and be specially woody plant trunk rot, dry rot, ring spot cutter dark standardized long circle around sick scar; intersect at middle part and draw number cutter; deeply reach health tissues or xylem; get 50 ~ 300ml Wound protective agent with the hairbrush libation at an ancient wedding ceremony again and smear disease scar; every 18 ~ 22 days once; check after continuous 2 times whether downright bad sick scar comes off; whether new organization grows, and according to circumstances smears again.
7. the using method of a kind of trees Wound protective agent according to claim 4; it is characterized in that: described in spread upon and cause trunk rot comparatively serious and produce the position of hole because insect pest or the hole place of rotting to cause are specially for sick worm harm; the wooden slag that first rotted in hole portion is wiped off; after rinsing with clear water; get 50 ~ 500ml Wound protective agent with the hairbrush libation at an ancient wedding ceremony again and smear whole sick portion and hole, after 15 days, carry out single treatment again.
8. the using method of a kind of trees Wound protective agent according to claim 4; it is characterized in that: described in spread upon bark damage cracking or the place that comes off and be specially bark cracking or bark local shedding; expose the ancient tree of xylem; first the bark of dry rot is cut away along exposed xylem edge with cutter; carve the xylem of hindering exposed part again; get 50 ~ 500ml Wound protective agent with the hairbrush libation at an ancient wedding ceremony to smear, once smear again after 28 ~ 30 days.
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CN106665240A (en) * 2016-11-30 2017-05-17 蚌埠市众星蔬果科技专业合作社联合社 Method for restoring rotten part of ancient metasequoia
CN107771870A (en) * 2017-11-23 2018-03-09 广西垚宇贸易有限公司 A kind of cinnamon bark protective agent and preparation method
CN107950602A (en) * 2017-12-14 2018-04-24 柳州市象行教育科技有限责任公司 A kind of special consolidant of callus and preparation method thereof
CN109041971A (en) * 2018-07-27 2018-12-21 南京林业大学 A kind of trees wound dermatoplastic method
CN109258177A (en) * 2018-11-12 2019-01-25 王君 A kind of restorative procedure of lacquer tree wound
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Publication number Priority date Publication date Assignee Title
CN106258443A (en) * 2016-09-06 2017-01-04 合肥元政农林生态科技有限公司 A kind of high-quality mountain cultural method
CN106665240A (en) * 2016-11-30 2017-05-17 蚌埠市众星蔬果科技专业合作社联合社 Method for restoring rotten part of ancient metasequoia
CN107771870A (en) * 2017-11-23 2018-03-09 广西垚宇贸易有限公司 A kind of cinnamon bark protective agent and preparation method
CN107950602A (en) * 2017-12-14 2018-04-24 柳州市象行教育科技有限责任公司 A kind of special consolidant of callus and preparation method thereof
CN109041971A (en) * 2018-07-27 2018-12-21 南京林业大学 A kind of trees wound dermatoplastic method
CN109258177A (en) * 2018-11-12 2019-01-25 王君 A kind of restorative procedure of lacquer tree wound
CN111955490A (en) * 2020-09-01 2020-11-20 开封市劳恩格润农业科技有限公司 Fruit tree wound healing agent and application method thereof

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