CN106070359B - A kind of plant seed germination box sterilization coating agent - Google Patents
A kind of plant seed germination box sterilization coating agent Download PDFInfo
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- CN106070359B CN106070359B CN201610525112.8A CN201610525112A CN106070359B CN 106070359 B CN106070359 B CN 106070359B CN 201610525112 A CN201610525112 A CN 201610525112A CN 106070359 B CN106070359 B CN 106070359B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/08—Amines; Quaternary ammonium compounds containing oxygen or sulfur
- A01N33/10—Amines; Quaternary ammonium compounds containing oxygen or sulfur having at least one oxygen or sulfur atom directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/0008—Organic ingredients according to more than one of the "one dot" groups of C08K5/01 - C08K5/59
- C08K5/0058—Biocides
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D105/00—Coating compositions based on polysaccharides or on their derivatives, not provided for in groups C09D101/00 or C09D103/00
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/14—Paints containing biocides, e.g. fungicides, insecticides or pesticides
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D7/00—Features of coating compositions, not provided for in group C09D5/00; Processes for incorporating ingredients in coating compositions
- C09D7/40—Additives
- C09D7/60—Additives non-macromolecular
- C09D7/63—Additives non-macromolecular organic
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of plant seed germination box sterilization coating agent, including 10 20 parts of Dopamine hydrochloride, 20 30 parts of pulullan polysaccharide, 50 60 parts of 95% ethyl alcohol, 20 30 parts of Herba Moslae Chinensis extract, 20 30 parts of Black Box Tracing extract.The coating agent is applied to germination box inboard wall of cartridge:Dopamine hydrochloride and pulullan polysaccharide are first dissolved in appropriate amounts of sterilized water, wiring solution-forming is brushed or dip-coating obtains primer coating in germination box inner wall.Then Herba Moslae Chinensis extract, Black Box Tracing extract are dissolved in 95% ethyl alcohol, brush or dip-coating obtains inter coat in germination box inner wall, is brushed again after dry or dip-coating obtains face coat.Coating agent of the present invention is harmless to vegetable seeds, can to germination box in bactericide-release, maintain germination box in cleaning sterile, effectively prevent seed go mouldy.
Description
Technical field
The present invention relates to a kind of plant seed germination boxes to sterilize coating agent, belongs to agricultural biological technical field.
Background technology
Seed germination experiment is the important content examined in seed chamber, is sowed to seed quality control and agricultural production
With meaning of crucial importance.Seed germination experiment is also a kind of method being commonly used in seed scientific research, such as degeneration-resistant experiment,
Cause experiment etc. to be required to grow up situation by germination and seedling to judge effect.In the paper bed germination test of conventional,
It is the germination paper of moistening to be laid on germination cassette bottom portion, then seed is placed on paper and is germinateed.
It is easily mouldy and the problem of infect due to often occurring seed in indoor seed germination experiment.Therefore it needs to seed and hair
The vessel such as bud box carry out disinfection processing.General common bactericidal agent has：75% alcohol 30s, 0.1% mercuric chloride 2-15min, 1%-
4% sodium hypochlorite 10-20min etc., or with 75% alcohol 30s+1% sodium hypochlorite 10min combined methods.100% or
The ability that alcohol of 95% alcohol than 70% or 75% penetrates bacterial spore is weak, and is not easy to kill them, therefore sterilizing is preferably selected
Select 70% or 75% alcohol.Mercuric chloride sterilizing power is strong, and irritation and corrosivity are big, and toxicity is also big, small to the permeability of bacterium, right
Metalware has corrosiveness, has severe toxicity to people and animals.Sodium hypochlorite sterilizing principle be to hydrolyze to form hypochlorous acid, hypochlorous acid again into
One step is decomposed to form nascent oxygen, and the extremely strong oxidisability of nascent oxygen makes the denaturation of the substances such as the protein on thalline and virus, from
And keep sex pheromone lethal.
It will be placed on germinating bed by the seed of sterilization, seed soaking, be then put into germination box cover in germinating box well
It germinates.Although by disinfecting, due to seed ingress of air during germination, it also will produce and go mouldy, it is general light
Micro- mouldy kind grain can be sorted out rinsed with clear water after put back to former germinating bed.Mouldy kind of grain it is more will replace germinating bed in time
Or germination container.This makes troubles to practical study.And existing fungicide is mostly chemical bactericide, long-term a large amount of uses
The problems such as there are potential toxic side effect and residual and environmental pollutions.
Invention content
For this purpose, the present invention provides a kind of plant seed germination box sterilization coating agent, it is applied to germination box inboard wall of cartridge, to plant
Seed is harmless, can to germination box in bactericide-release, maintain germination box in cleaning sterile, effectively prevent seed go mouldy.
For this purpose, the present invention is as follows using technical solution, a kind of plant seed germination box sterilization coating agent, including hydrochloric acid DOPA
10-20 parts of amine, 20-30 parts of pulullan polysaccharide, 50-60 parts of 95% ethyl alcohol, 20-30 parts of Herba Moslae Chinensis extract, Black Box Tracing
20-30 parts of extract.
The Herba Moslae Chinensis extract extracting method is：Herba Moslae Chinensis drying and crushing sieves with 100 mesh sieve, with heat at 65 DEG C of absolute methanol
Refluxing extraction 3 times, each 2h, solid-liquid ratio 1:15, merge extracting solution and is concentrated under reduced pressure at medicinal extract shape to constant weight, with acetone water 1:1
It after being completely dissolved, then is extracted successively with isometric petroleum ether, chloroform, ethyl acetate, n-butanol, extract liquor is concentrated under reduced pressure
At medicinal extract to constant weight, it is dried and crushed into extract powder.
The Black Box Tracing extract extracting method is：Black Box Tracing fruit and/or blade drying and crushing mistake
100 mesh sieve, with without circumfluence distillation 3 times, each 2h at 65 DEG C of 70% ethyl alcohol, solid-liquid ratio 1:10, it is dense to merge extracting solution decompression
Medicinal extract shape is shortened into constant weight, with acetone water 1:After 1 is completely dissolved, then successively with isometric petroleum ether, chloroform, ethyl acetate, just
Butanol is extracted, and extract liquor is concentrated under reduced pressure into medicinal extract to constant weight, extract powder is dried and crushed into.
Dopamine hydrochloride and pulullan polysaccharide are first dissolved in appropriate amounts of sterilized water, wiring solution-forming is brushed or dip-coating is in germination box
Wall obtains primer coating.Then Herba Moslae Chinensis extract, Black Box Tracing extract are dissolved in 95% ethyl alcohol, brushing or dip-coating
Inter coat is obtained in germination box inner wall, is brushed again after dry or dip-coating obtains face coat.
Herba Moslae Chinensis is a kind of Chinese medicine, is worked hard, temperature；Major function：Driving away summer heat, promoting blood circulation, qi-regulating, dampness elimination.Xia Yue flu are controlled,
Heatstroke evil of vomitting, abdominal pain diarrhea, bruise stasis caused pain, eczema, furuncle.Research shows that Herba Moslae Chinensis contains volatile oil about 0.7%, carvacrol
65%, carvacrol acetic acid esters 6% still has cymol, α-thujene, linalool, borneol, α-carypohyllene etc..
Black Box Tracing also known as the wild cherry certain kind of berries, the not old certain kind of berries, the rose family, gland Aronia, perennial machaka.Its fruit
And its extract has special curative effect to cardiovascular and cerebrovascular diseases such as heart disease, hypertension, is widely used in curing in European and American areas
Medicine and functional food industry.Anthocyanidin, flavones (fresh fruit content is up to 0.25%-0.35%), polyphenol are known plants in fruit
Middle content is highest.Fruit also contains the substances such as multivitamin and mineral element.More Black Box Tracing fruits rich in Vc and
Antioxidant (Beta Carotene (carrotene)).Fruit extract has well treatment Radiation sickness and heavy metal poisoning disease
Curative effect.
Present invention research finds that Herba Moslae Chinensis extract has the function of inhibiting and killing mould well.It is spent with black fruit gland rib
Chinese catalpa extract is used together with good bactericidal anti-oxidation efficacy.When coating agent of the present invention is applied to germination box inner wall, adopt
Sterilization coating agent can be made to adhere to well using Dopamine hydrochloride and pulullan polysaccharide as bottom brushing with layering painting way
On germination box inner wall, Herba Moslae Chinensis extract, Black Box Tracing extract brush at twice make it have it is lasting good
Bactericidal and bacteriostatic effect.
The present invention has the advantages that：
(1) existing fungicide is mostly chemical bactericide, but its own there are potential toxic side effect and residual and
Environmental pollution.And fungicide of the present invention is biological bactericide, has excellent sterilization, antioxygenic property, it is safer, have
Effect.It is safe and harmless to vegetable seeds, it does not influence to germinate.
(2) using the germination box of present invention sterilization coating agent, it is possible to reduce the time of seed disinfection processing early period, accelerate examination
Test process.It is demonstrated experimentally that seed without disinfecting for a long time, is put into the germination for being coated with sterilization coating agent after simple process
In box, you can prevent the mold of seed well, improve experiment accuracy rate and efficiency.
Specific implementation mode
Technical scheme of the present invention and its generated technique effect are done further with reference to specific implementation method
Illustrate, the following description is only intended to explain the invention, but is not limited in any way to the present invention, be based on present invention teach that
Made by it is any transform or replace, all belong to the scope of protection of the present invention.Method used in the present invention unless otherwise specified,
It is this field conventional method.Test material as used in the following examples, reagent etc. unless otherwise specified can be from business
Approach obtains.
A kind of 1. plant seed germination box of embodiment sterilizes coating agent, including 15 parts of Dopamine hydrochloride, pulullan polysaccharide 25
Part, 95% 55 parts of ethyl alcohol, 25 parts of Herba Moslae Chinensis extract, 25 parts of Black Box Tracing extract.
Herba Moslae Chinensis extract extracting method is：Herba Moslae Chinensis drying and crushing sieves with 100 mesh sieve, and is flowed back with heat at 65 DEG C of absolute methanol
Extraction 3 times, each 2h, solid-liquid ratio 1:15, merge extracting solution and is concentrated under reduced pressure at medicinal extract shape to constant weight, with acetone water 1:1 completely
It after dissolving, then is extracted successively with isometric petroleum ether, chloroform, ethyl acetate, n-butanol, extract liquor is concentrated under reduced pressure into leaching
Cream is dried and crushed into extract powder to constant weight.
Black Box Tracing extract extracting method is：Black Box Tracing fruit and/or blade drying and crushing cross 100 mesh
Sieve, with without circumfluence distillation 3 times, each 2h at 65 DEG C of 70% ethyl alcohol, solid-liquid ratio 1:10, merge extracting solution and is concentrated under reduced pressure into leaching
Paste is to constant weight, with acetone water 1:After 1 is completely dissolved, then successively with isometric petroleum ether, chloroform, ethyl acetate, n-butanol into
Row extraction, extract liquor is concentrated under reduced pressure into medicinal extract to constant weight, extract powder is dried and crushed into.
Coating agent of the present invention is in the box that germinates：Dopamine hydrochloride and pulullan polysaccharide are first dissolved in appropriate amounts of sterilized water, matched
It is brushed at solution or dip-coating obtains primer coating in germination box inner wall.Then Herba Moslae Chinensis extract, Black Box Tracing are extracted
Object is dissolved in 95% ethyl alcohol, brushes or dip-coating obtains inter coat in germination box inner wall, brushed again after dry or dip-coating obtains
Face coat.
A kind of 2. plant seed germination box of embodiment sterilizes coating agent, including 25 parts of Dopamine hydrochloride, pulullan polysaccharide 20
Part, 95% 50 parts of ethyl alcohol, 20 parts of Herba Moslae Chinensis extract, 20 parts of Black Box Tracing extract.
Method of preparation and use is the same as embodiment 1.
A kind of 3. plant seed germination box of embodiment sterilizes coating agent, including 20 parts of Dopamine hydrochloride, pulullan polysaccharide 30
Part, 95% 60 parts of ethyl alcohol, 30 parts of Herba Moslae Chinensis extract, 30 parts of Black Box Tracing extract.
Method of preparation and use is the same as embodiment 1.
A kind of 4. plant seed germination box of embodiment sterilizes coating agent, including 16 parts of Dopamine hydrochloride, pulullan polysaccharide 28
Part, 95% 56 parts of ethyl alcohol, 27 parts of Herba Moslae Chinensis extract, 22 parts of Black Box Tracing extract.
Method of preparation and use is the same as embodiment 1.
1. Herba Moslae Chinensis extract of test example studies the killing effect of mould
One, method
1, Herba Moslae Chinensis extracting solution prepares
Herba Moslae Chinensis through drying, crush, 5 grams are taken after being fully ground, with circumfluence distillation at 65 DEG C of absolute methanol 3 times, every time
2h, solid-liquid ratio 1:15, merge extracting solution and is concentrated under reduced pressure at medicinal extract shape to constant weight, with acetone water 1:After 1 is completely dissolved, then successively
It is extracted with isometric petroleum ether, chloroform, ethyl acetate, n-butanol, extract liquor, which is concentrated under reduced pressure into extracting liquid volume, is
50ml。
2, the gradient dilution of Herba Moslae Chinensis extracting solution
Dilute 5 concentration gradients according to this with sterile water, i.e., concentration be respectively 100,50,25,12.5,6.25mg/ml
3, the preparation of mycotic spore suspension
The mould silk of head mold, Mucor, mould, aspergillus is inoculated on culture medium respectively, cultivates 72h.It waits being paved with entire training
It after supporting ware, is rinsed 3 times with PBS, through filtered through gauze, M199 cell culture fluids is added and adjust separately spore concentration through cell count
1×104、1×106、1×108A/ml
4, mycotic spore inhibits experiment
The Herba Moslae Chinensis extracting solution 2ml of various concentration adds to PAD culture mediums of the 10ml through dissolving, and shakes up, is poured into training rapidly
It supports for use in ware.The 1/10 of a concentration of former experimental liquid concentration of PAD tablets.
1ul spores bacterium solution on the culture medium of solidification, 5d is cultivated at 25 DEG C by multipoint inoculation.It is every in incubation period to see for 24 hours
It examines 1 time.Each concentration sets two parallel controls, while setting and the blank PAD tablets of sterile water is added to do blank control.
5, fungal hyphae kills experiment
Will be after 25 DEG C of culture 72h of PAD culture mediums, the mould for being paved with tablet is cut into about 1cm with sterile razor blade2It is small
Block, the fritter cut is put into the test tube for the Herba Moslae Chinensis extracting solution for filling any of the above various concentration gradient impregnate 0.5h, 1h,
2h takes out the fritter impregnated with aseptic nipper and is placed in culture, 25 DEG C of culture observation 5d, primary per observing for 24 hours in incubation period,
Visually judgement is not born without mould long, indicates that extracting solution has killing effect under the concentration action time to mould.
Each concentration sets 2 parallel controls, while setting and doing blank control through the fritter that sterile water impregnated.
Two, result
1, inhibition of the Herba Moslae Chinensis extracting solution to mycotic spore
Herba Moslae Chinensis extracting solution has good inhibiting effect in tested concentration range to four kinds of mycotics spore, and is testing
Fungistatic effect indifference within the scope of spore suspension.It the results are shown in Table 1.
MIC of the 1. Herba Moslae Chinensis extracting solution of table to different mycotics spore
| 1×104A/ml | 1×106A/ml | 1×108A/ml | |
| Head mold | 1.2-2.5 | 1.2-2.5 | 1.2-2.6 |
| Mucor | 1.5-2.8 | 1.5-2.9 | 1.5-3.0 |
| Mould | 1.25-2.45 | 1.25-2.45 | 1.25-2.5 |
| Aspergillus | 2.1-3.2 | 2.2-3.2 | 2.2-3.3 |
2, killing effect of the Herba Moslae Chinensis extracting solution to fungal hyphae
Herba Moslae Chinensis extracting solution has good killing effect to fungal hyphae in this experimental concentration range.As a result such as table 2
It is shown.
The killing concentration of 2. Herba Moslae Chinensis extracting solution pair of table, four kinds of mould silks and action time
Note：+ indicate fungus growth；Indicate that mould does not grow
Three, it discusses
Mainly caused by the moulds such as head mold, Mucor, aspergillus, mould, this experiment proves Herba Moslae Chinensis extraction for the mold of seed
Liquid has good inhibition and killing effect to above-mentioned four kinds of moulds.Illustrate that Herba Moslae Chinensis extracting solution can effectively prevent the mold of seed
And it rots.
Test example two, the different influences disinfected to seed disinfection effect and sprouting
One, method
1, using 958 seeds of Zheng Dan as experiment material, using method between BP paper：According to《Crop seeds inspection procedure》Standard is sent out
Bud test method carries out.(1) two layers of filter paper is laid in germination box, is soaked completely with 20ml aqua sterilisas.(2) 50 are uniformly sowed
Grain seed.(3) the big filter paper such as one is covered.(4) it is put into incubator and cultivates.Every other day supplement 20ml aqua sterilisas.
2, it is grouped
Totally four groups, every group of 2 repetitions.
I groups：Seed was with 30% hypochlorite disinfectant 30 minutes, and commonly germinate box；
II groups：Seed is coated with the germination box of present invention sterilization coating agent with 30% hypochlorite disinfectant 30 minutes；
III groups：Seed was with 30% hypochlorite disinfectant 5 minutes, and commonly germinate box；
IV groups：Seed was with 30% hypochlorite disinfectant 5 minutes；It is coated with the germination box of present invention sterilization coating agent.
Other processing all sames.
3, counting statistics
According to《Crop seeds inspection procedure》Defined method of counting, after 7 days, to normal seedling, lopsided seedling, mouldy
Seed, rot seed and chitting piece does not count respectively, and test result is indicated with the percentage of the total grain number of seed.
Two, result
1, percentage of seedgermination situation is shown in Table 3
Table 3, influence of the different disposal to percentage of seedgermination
It can be seen from the results above that I groups and II groups, III groups and IV groups compare two-by-two, with coating agent of the present invention
The test group of germination box, mouldy and mildew and rot seed is minimum, germination percentage highest.I groups are compared with III groups, are illustrated with 30% hypochlorous acid
Sodium disinfecting time is reduced, and the mold of seed and mildew and rot quantity increase.And II groups are compared with IV groups, although with 30% hypochlorite disinfectant
Time is reduced, but does not influence percentage of seedgermination.I groups are compared with IV groups, and IV groups are reduced with hypochlorite disinfectant's time, but
Because use the present invention sterilization coating kit, can during germination sustained release Fungicidal substance, so ensure
Clean environment in germination box, the mold of seed or mildew and rot quantity is few, and whole germination percentage and group I are similar.
The studies above shows coating agent of the present invention in the box that germinates, both can effectively prevent the mold of seed and go rotten, also
The seed disinfection time can be greatly reduced, save the time, improve test efficiency.
Claims (4)
1. a kind of plant seed germination box sterilizes coating agent, characterized in that including 10-20 parts of Dopamine hydrochloride, pulullan polysaccharide
20-30 parts, 50-60 parts of 95% ethyl alcohol, 20-30 parts of Herba Moslae Chinensis extract, 20-30 parts of Black Box Tracing extract.
2. plant seed germination box as described in claim 1 sterilizes coating agent, characterized in that Herba Moslae Chinensis extract extraction side
Method is：Herba Moslae Chinensis drying and crushing sieves with 100 mesh sieve, with circumfluence distillation 3 times, each 2h at 65 DEG C of absolute methanol, solid-liquid ratio 1:
15, merge extracting solution and is concentrated under reduced pressure at medicinal extract shape to constant weight, with acetone water 1:After 1 is completely dissolved, then successively with isometric oil
Ether, chloroform, ethyl acetate, n-butanol are extracted, and extract liquor is concentrated under reduced pressure into medicinal extract to constant weight, extract is dried and crushed into
Powder.
3. plant seed germination box as described in claim 1 sterilizes coating agent, characterized in that the Black Box Tracing extract
Extracting method is：Black Box Tracing fruit and/or blade drying and crushing sieve with 100 mesh sieve, and are returned with without heat at 65 DEG C of 70% ethyl alcohol
Stream extraction 3 times, each 2h, solid-liquid ratio 1:10, merge extracting solution and is concentrated under reduced pressure at medicinal extract shape to constant weight, with acetone water 1:1 is complete
After fully dissolved, then extracted successively with isometric petroleum ether, chloroform, ethyl acetate, n-butanol, by extract liquor be concentrated under reduced pressure at
Medicinal extract is dried and crushed into extract powder to constant weight.
4. plant seed germination box sterilization coating agent described in claim 1 is applied in germination box, characterized in that step is such as
Under：Dopamine hydrochloride and pulullan polysaccharide are first dissolved in appropriate amounts of sterilized water, wiring solution-forming is brushed or dip-coating is obtained in germination box inner wall
To primer coating；Then Herba Moslae Chinensis extract, Black Box Tracing extract are dissolved in 95% ethyl alcohol, brush or dip-coating is being sent out
Bud box inner wall obtains inter coat, is brushed again after dry or dip-coating obtains face coat.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101233867A (en) * | 2008-02-26 | 2008-08-06 | 天津市恒源伟业生物科技发展有限公司 | Matrine agent plant pesticide and preparation thereof |
| CN103783093A (en) * | 2014-03-13 | 2014-05-14 | 四川龙蟒福生科技有限责任公司 | Composition having functions of sterilization and nematode prevention and control |
| CN104524615A (en) * | 2014-12-11 | 2015-04-22 | 东莞市日友汽车用品有限公司 | Air freshener and preparation method thereof |
| CN105767029A (en) * | 2014-12-22 | 2016-07-20 | 重庆市万盛经开区黑山镇供销合作社有限公司 | Chinese herbal disinfector formulation |
-
2016
- 2016-07-05 CN CN201610525112.8A patent/CN106070359B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101233867A (en) * | 2008-02-26 | 2008-08-06 | 天津市恒源伟业生物科技发展有限公司 | Matrine agent plant pesticide and preparation thereof |
| CN103783093A (en) * | 2014-03-13 | 2014-05-14 | 四川龙蟒福生科技有限责任公司 | Composition having functions of sterilization and nematode prevention and control |
| CN104524615A (en) * | 2014-12-11 | 2015-04-22 | 东莞市日友汽车用品有限公司 | Air freshener and preparation method thereof |
| CN105767029A (en) * | 2014-12-22 | 2016-07-20 | 重庆市万盛经开区黑山镇供销合作社有限公司 | Chinese herbal disinfector formulation |
Non-Patent Citations (2)
| Title |
|---|
| "响应面优化黑果腺肋花楸多酚提取工艺及抗氧化活性研究";李斌，;《中国食品科学技术学会第十二届年会暨第八届中美食品业高层论坛论文摘要集》;20151021;第104页 * |
| "石香薷提取物抑制黄曲霉机制的初步研究";蒋红梅 等，;《中国食品学报》;20070815;第2.1.2节，第3.1节，表1 * |
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| Publication number | Publication date |
|---|---|
| CN106070359A (en) | 2016-11-09 |
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