CN106070359A - A kind of plant seed germination box sterilization coating agent - Google Patents
A kind of plant seed germination box sterilization coating agent Download PDFInfo
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- CN106070359A CN106070359A CN201610525112.8A CN201610525112A CN106070359A CN 106070359 A CN106070359 A CN 106070359A CN 201610525112 A CN201610525112 A CN 201610525112A CN 106070359 A CN106070359 A CN 106070359A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/08—Amines; Quaternary ammonium compounds containing oxygen or sulfur
- A01N33/10—Amines; Quaternary ammonium compounds containing oxygen or sulfur having at least one oxygen or sulfur atom directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/0008—Organic ingredients according to more than one of the "one dot" groups of C08K5/01 - C08K5/59
- C08K5/0058—Biocides
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D105/00—Coating compositions based on polysaccharides or on their derivatives, not provided for in groups C09D101/00 or C09D103/00
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/14—Paints containing biocides, e.g. fungicides, insecticides or pesticides
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D7/00—Features of coating compositions, not provided for in group C09D5/00; Processes for incorporating ingredients in coating compositions
- C09D7/40—Additives
- C09D7/60—Additives non-macromolecular
- C09D7/63—Additives non-macromolecular organic
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Materials Engineering (AREA)
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- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of plant seed germination box sterilization coating agent, including dopamine hydrochloride 10 20 parts, pulullan polysaccharide 20 30 parts, 95% ethanol 50 60 parts, stone perfume (or spice) extract 20 30 parts, Black Box Tracing extract 20 30 parts.This coating agent is applied to germination box inboard wall of cartridge: dopamine hydrochloride and pulullan polysaccharide are first dissolved in appropriate amounts of sterilized water, and wiring solution-forming is brushed or dip-coating obtains primer coating at germination box inwall.Then stone perfume (or spice) extract, Black Box Tracing extract are dissolved in 95% ethanol, brush or dip-coating obtains inter coat at germination box inwall, the most again brush or dip-coating obtains face coat.Coating agent of the present invention is harmless to plant seed, can in germination box bactericide-release, maintain cleaning sterile in germination box, effectively prevention seed goes mouldy.
Description
Technical field
The present invention relates to a kind of plant seed germination box sterilization coating agent, belong to agricultural biological technical field.
Background technology
Seed germination experiment is an important content of the interior inspection of seed chamber, sows seed quality control and agricultural production
There is meaning of crucial importance.Seed germination experiment is also a kind of method commonly used in seed scientific research, such as degeneration-resistant test,
Cause test etc. to be required to by germination and seedling growth situation and judge effect.In the paper bed germination test of conventional,
It is germination cassette bottom portion that moistening germination paper is laid on, then seed is placed on paper germination.
Owing to often there is the problem that seed is the most mouldy and infects in indoor seed germination experiment.It is thus desirable to seed and send out
The vessel disinfections such as bud box.General conventional biocide has: 75% ethanol 30s, 0.1% mercuric chloride 2-15min, 1%-
4% sodium hypochlorite 10-20min etc., or the ethanol 30s+1% sodium hypochlorite 10min combined method with 75%.100% or
The ability that the ethanol of 95% ethanol than 70% or 75% penetrates bacterial spore is weak, and is difficult to kill them, therefore sterilizing is preferably selected
Select the ethanol of 70% or 75%.Mercuric chloride sterilizing power is strong, and zest and corrosivity are big, and toxicity is also big, little to the permeability of antibacterial, right
Metalware has corrosiveness, and people and animals are had severe toxicity.The principle of sodium hypochlorite sterilizing is to hydrolyze to form hypochlorous acid, and hypochlorous acid enters again
One step is decomposed to form nascent oxygen, and the extremely strong oxidisability of nascent oxygen makes the material degeneration such as the protein in thalline and virus, from
And make sex pheromone lethal.
Seed through sterilization, seed soaking is placed on germinating bed, then germination lid is put into germinating box well
Germinate.Although through disinfecting, due to seed ingress of air during germinateing, also producing and go mouldy, the lightest
Micro-mouldy kind grain can be sorted out after rinsing with clear water and put back to former germinating bed.What mouldy kind grain was more will change germinating bed in time
Or germination container.This makes troubles to practical study.And existing antibacterial is mostly chemical bactericide, long-term a large amount of use
There is potential toxic and side effects and the problem such as residual and environmental pollution.
Summary of the invention
To this end, the present invention provides a kind of plant seed germination box sterilization coating agent, it is applied to germination box inboard wall of cartridge, to plant
Seed is harmless, can in germination box bactericide-release, maintain cleaning sterile in germination box, effectively prevention seed goes mouldy.
To this end, the present invention uses technical scheme as follows, a kind of plant seed germination box sterilization coating agent, including hydrochloric acid DOPA
Amine 10-20 part, pulullan polysaccharide 20-30 part, 95% ethanol 50-60 part, stone perfume (or spice) extract 20-30 part, Black Box Tracing
Extract 20-30 part.
Described stone perfume (or spice) extract extracting method is: stone perfume (or spice) drying and crushing crosses 100 mesh sieves, by heat at absolute methanol 65 DEG C
Reflux, extract, 3 times, each 2h, solid-liquid ratio is 1:15, united extraction liquid concentrating under reduced pressure become extractum shape to constant weight, with acetone water 1:1
After being completely dissolved, then extract with equal-volume petroleum ether, chloroform, ethyl acetate, n-butyl alcohol successively, by extract concentrating under reduced pressure
Become extractum to constant weight, be dried and crushed into extract powder.
Described Black Box Tracing extract extracting method is: Black Box Tracing fruit and/or blade drying and crushing mistake
100 mesh sieves, with circumfluence distillation 3 times, each 2h at nothing 70% ethanol 65 DEG C, solid-liquid ratio is 1:10, and the decompression of united extraction liquid is dense
Shorten extractum shape into constant weight, after being completely dissolved with acetone water 1:1, more successively by equal-volume petroleum ether, chloroform, ethyl acetate, just
Butanol extracts, and becomes extractum to constant weight extract concentrating under reduced pressure, is dried and crushed into extract powder.
Dopamine hydrochloride and pulullan polysaccharide are first dissolved in appropriate amounts of sterilized water, and wiring solution-forming is brushed or dip-coating is in germination box
Wall obtains primer coating.Then stone perfume (or spice) extract, Black Box Tracing extract are dissolved in 95% ethanol, brush or dip-coating
Obtain inter coat at germination box inwall, the most again brush or dip-coating obtains face coat.
Shi Xiang is a kind of Chinese crude drug, and it is worked hard, temperature；Function cures mainly: drive away summer heat, and invigorates blood circulation, and regulates the flow of vital energy, removing dampness.Control Xia Yue flu,
Heatstroke vomiting and nausea, stomachache has loose bowels, traumatic injury stasis of blood pain, eczema, furuncle and phyma.Research shows that Shi Xiang contains volatile oil about 0.7%, carvacrol
65%, carvacrol acetas 6%, still has cymol, α-thujene, linalool, Borneolum Syntheticum, α-caryophyllene etc..
Black Box Tracing has another name called the wild cherry certain kind of berries, Aronia melanocarpa, Rosaceae, gland Aronia, perennial machaka.Its fruit
And extract has special curative effect to the cardiovascular and cerebrovascular disease such as heart disease, hypertension, it is widely used in doctor in European and American areas
Medicine and functional food industry.In fruit, anthocyanidin, flavone (fresh fruit content is up to 0.25%-0.35%), polyphenol are known plants
Middle content is the highest.Fruit is possibly together with the material such as multivitamin and mineral element.Many Black Box Tracing fruit rich in Vc and
Antioxidant (Beta Carotene (carotene)).Treatment Radiation sickness and heavy metal poisoning disease are had well by fruit extract
Curative effect.
The present invention studies discovery stone perfume (or spice) extract and has the effect well suppressing and killing mycete.With Radix osteomelis schwerinais gland rib flower
Chinese catalpa extract is used together has good bactericidal anti-oxidation efficacy.When coating agent of the present invention is applied to germination box inwall, adopt
By layering painting way, using dopamine hydrochloride and pulullan polysaccharide as bottom brushing, sterilization coating agent can be made well to adhere to
On germination box inwall, stone perfume (or spice) extract, Black Box Tracing extract brush at twice make it have lasting good
Bactericidal and bacteriostatic effect.
There is advantages that
(1) existing antibacterial is mostly chemical bactericide, but himself exist potential toxic and side effects and residual and
Environmental pollution.And antibacterial of the present invention is biofungicide, there is the sterilization of excellence, antioxygenic property, safer, have
Effect.Safe and harmless to plant seed, do not affect germination.
(2) present invention is used to sterilize the germination box of coating agent, it is possible to reduce the time that early stage seed disinfection processes, to accelerate examination
Test process.It is demonstrated experimentally that seed is without disinfecting for a long time, after simple process, put into the germination scribbling sterilization coating agent
In box, the mold of seed be can well prevent, test accuracy rate and efficiency improved.
Detailed description of the invention
Below in conjunction with specific implementation method, technical scheme and produced technique effect thereof are done further
Illustrating, the description below is merely to explain the present invention, but is any limitation as the present invention never in any form, based on present invention teach that
Any conversion made or replacement, belong to protection scope of the present invention.Method used in the present invention if no special instructions,
It is this area conventional method.Test material used in following embodiment, reagent etc., if no special instructions, all can be from business
Approach obtains.
1. 1 kinds of plant seed germination box sterilization coating agents of embodiment, including dopamine hydrochloride 15 parts, pulullan polysaccharide 25
Part, 95% ethanol 55 parts, stone perfume (or spice) extract 25 parts, Black Box Tracing extract 25 parts.
Stone perfume (or spice) extract extracting method is: stone perfume (or spice) drying and crushing crosses 100 mesh sieves, with hot reflux at absolute methanol 65 DEG C
Extracting 3 times, each 2h, solid-liquid ratio is 1:15, united extraction liquid concentrating under reduced pressure become extractum shape to constant weight, complete with acetone water 1:1
After dissolving, then extract with equal-volume petroleum ether, chloroform, ethyl acetate, n-butyl alcohol successively, extract concentrating under reduced pressure is become leaching
Cream, to constant weight, is dried and crushed into extract powder.
Black Box Tracing extract extracting method is: Black Box Tracing fruit and/or blade drying and crushing cross 100 mesh
Sieve, with circumfluence distillation 3 times, each 2h at nothing 70% ethanol 65 DEG C, solid-liquid ratio is 1:10, and united extraction liquid concentrating under reduced pressure becomes leaching
Paste is to constant weight, after being completely dissolved with acetone water 1:1, then enters with equal-volume petroleum ether, chloroform, ethyl acetate, n-butyl alcohol successively
Row extraction, becomes extractum to constant weight extract concentrating under reduced pressure, is dried and crushed into extract powder.
Coating agent of the present invention is used for germinateing in box: first dopamine hydrochloride and pulullan polysaccharide is dissolved in appropriate amounts of sterilized water, joins
Become solution to brush or dip-coating obtains primer coating at germination box inwall.Then stone perfume (or spice) extract, Black Box Tracing are extracted
Thing is dissolved in 95% ethanol, brushes or dip-coating obtains inter coat at germination box inwall, the most again brushes or dip-coating obtains
Face coat.
2. 1 kinds of plant seed germination box sterilization coating agents of embodiment, including dopamine hydrochloride 25 parts, pulullan polysaccharide 20
Part, 95% ethanol 50 parts, stone perfume (or spice) extract 20 parts, Black Box Tracing extract 20 parts.
Method of preparation and use is with embodiment 1.
3. 1 kinds of plant seed germination box sterilization coating agents of embodiment, including dopamine hydrochloride 20 parts, pulullan polysaccharide 30
Part, 95% ethanol 60 parts, stone perfume (or spice) extract 30 parts, Black Box Tracing extract 30 parts.
Method of preparation and use is with embodiment 1.
4. 1 kinds of plant seed germination box sterilization coating agents of embodiment, including dopamine hydrochloride 16 parts, pulullan polysaccharide 28
Part, 95% ethanol 56 parts, stone perfume (or spice) extract 27 parts, Black Box Tracing extract 22 parts.
Method of preparation and use is with embodiment 1.
The killing action of mycete is studied by test example 1. stone perfume (or spice) extract
One, method
1, stone perfume (or spice) extracting solution prepares
Stone perfume (or spice) drying, pulverizing, take 5 grams after being fully ground, with circumfluence distillation 3 times at absolute methanol 65 DEG C, every time
2h, solid-liquid ratio is 1:15, united extraction liquid concentrating under reduced pressure become extractum shape to constant weight, after being completely dissolved with acetone water 1:1, more successively
Extracting with equal-volume petroleum ether, chloroform, ethyl acetate, n-butyl alcohol, extract is evaporated to extracting liquid volume is
50ml。
2, the gradient dilution of stone perfume (or spice) extracting solution
Dilute according to this with sterilized water 5 Concentraton gradient, i.e. concentration be respectively 100,50,25,12.5,6.25mg/ml
3, the preparation of mycotic spore suspension
Respectively by rhizopus, Mucor, penicillium sp, aspergillosis mould mycelium inoculation in culture medium, cultivate 72h.Wait to be paved with whole training
After supporting ware, rinse 3 times with PBS, through filtered through gauze, add M199 cell culture fluid, through cell counting, adjust spore concentration respectively
1×104、1×106、1×108Individual/ml
4, mycotic spore inhibition test
The stone perfume (or spice) extracting solution 2ml of variable concentrations adds to the 10ml PAD culture medium through dissolving, and shakes up, is poured into rapidly training
Support in ware stand-by.PAD flat board concentration is the 1/10 of former experimental liquid concentration.
1ul spore bacterium solution in the culture medium of solidification, is cultivated 5d by multipoint inoculation at 25 DEG C.In incubation period, every 24h sees
Examine 1 time.Each concentration sets two parallel controls, sets simultaneously and adds the blank PAD flat board of sterilized water and do blank.
5, fungal hyphae kills test
Will be after PAD culture medium 25 DEG C to cultivate 72h, the mycete sterile razor blade being paved with flat board is cut into about 1cm2Little
Block, the fritter cut is put in the test tube of the stone perfume (or spice) extracting solution filling any of the above variable concentrations gradient soak 0.5h, 1h,
2h, takes out, with aseptic nipper, the fritter soaked and is placed in cultivation, cultivates for 25 DEG C and observes 5d, and in incubation period, every 24h observes once,
Naked eyes judge not without mould colony growth, represent that extracting solution has killing action to mycete under this concentration this action time.
Each concentration sets 2 parallel controls, sets the fritter soaked through sterilized water simultaneously and does blank.
Two, result
1, the stone perfume (or spice) extracting solution suppression to mycotic spore
Stone perfume (or spice) extracting solution has good inhibiting effect in tested concentration range to four kinds of mycotics spore, and in test
Fungistatic effect indifference in the range of spore suspension.The results are shown in Table 1.
The table 1. stone perfume (or spice) extracting solution MIC to different mycotics spore
| 1×10<sup>4</sup>Individual/ml | 1×10<sup>6</sup>Individual/ml | 1×10<sup>8</sup>Individual/ml | |
| Rhizopus | 1.2-2.5 | 1.2-2.5 | 1.2-2.6 |
| Mucor | 1.5-2.8 | 1.5-2.9 | 1.5-3.0 |
| Penicillium sp | 1.25-2.45 | 1.25-2.45 | 1.25-2.5 |
| Aspergillosis | 2.1-3.2 | 2.2-3.2 | 2.2-3.3 |
2, the stone perfume (or spice) extracting solution killing action to fungal hyphae
In the range of this experimental concentration, stone perfume (or spice) extracting solution has good killing action to fungal hyphae.Result such as table 2
Shown in.
Four kinds of mould mycelia are killed concentration and action time by table 2. stone perfume (or spice) extracting solution
Note :+represent fungus growth；-represent that mycete does not grow
Three, discuss
The mold of seed is mainly mould microbial by rhizopus, Mucor, aspergillosis, penicillium sp etc., and this test proves that stone perfume (or spice) extracts
Liquid all has good suppression and killing action to above-mentioned four kinds of mycetes.Illustrate that stone perfume (or spice) extracting solution can effectively prevent the mold of seed
And rot.
Test example two, difference disinfect the impact on seed disinfection effect and sprouting
One, method
1, with Zheng Dan 958 seed as experiment material, method between BP paper is used: send out according to " crop seeds inspection procedure " standard
Bud test method is carried out.(1) two layers of filter paper is laid in germination box, soaks completely with 20ml aquesterilisa.(2) 50 are uniformly sowed
Grain seed.(3) a big filter paper such as grade is covered.(4) put in incubator and cultivate.Every other day supplement 20ml aquesterilisa.
2, packet
Totally four groups, often 2 repetitions of group.
I group: seed was with 30% hypochlorite disinfectant 30 minutes, and commonly germinate box；
II group: seed with 30% hypochlorite disinfectant 30 minutes, scribbles the present invention and sterilizes the germination box of coating agent；
III group: seed was with 30% hypochlorite disinfectant 5 minutes, and commonly germinate box；
IV group: seed was with 30% hypochlorite disinfectant 5 minutes；Scribble the present invention to sterilize the germination box of coating agent.
Other process are the most identical.
3, counting statistics
The method of counting specified according to " crop seeds inspection procedure ", after 7 days, to normal seedling, deformity seedling, mouldy
Seed, rot seed and not chitting piece count respectively, result of the test represents with the percentage rate of seed total grain number.
Two, result
1, percentage of seedgermination situation, is shown in Table 3
Table 3, the different disposal impact on percentage of seedgermination
It can be seen from the results above that I group and II group, III group and IV group contrast two-by-two, with coating agent of the present invention
The test group of germination box, mouldy and mildew and rot seed is minimum, germination percentage is the highest.I group is compared with III group, illustrates to use 30% hypochlorous acid
Sodium disinfecting time reduces, the mold of seed and mildew and rot increasing number.And II group is compared with IV group, although use 30% hypochlorite disinfectant
Time reduces, but does not have influence on percentage of seedgermination.I group is compared with IV group, and IV group reduced with hypochlorite disinfectant's time, but
Because have employed the present invention to sterilize the test kit of coating, it is possible to sustained release Fungicidal substance during germination, so ensureing
Clean environment in germination box, the mold of seed or mildew and rot quantity is few, and overall germination percentage is with to organize I similar.
The studies above shows that coating agent of the present invention, in the box that germinates, both can effectively have been prevented and treated the mold of seed and go rotten, also
The seed disinfection time can be greatly reduced, save the time, improve test efficiency.
Claims (4)
1. a plant seed germination box sterilization coating agent, is characterized in that, including dopamine hydrochloride 10-20 part, pulullan polysaccharide
20-30 part, 95% ethanol 50-60 part, stone perfume (or spice) extract 20-30 part, Black Box Tracing extract 20-30 part.
2. plant seed germination box sterilization coating agent as claimed in claim 1, is characterized in that, described stone perfume (or spice) extract extraction side
Method is: stone perfume (or spice) drying and crushing crosses 100 mesh sieves, and with circumfluence distillation 3 times at absolute methanol 65 DEG C, each 2h, solid-liquid ratio is 1:
15, united extraction liquid concentrating under reduced pressure becomes extractum shape to constant weight, after being completely dissolved with acetone water 1:1, then uses equal-volume oil successively
Ether, chloroform, ethyl acetate, n-butyl alcohol extract, and become extractum to constant weight extract concentrating under reduced pressure, are dried and crushed into extract
Powder.
3. plant seed germination box sterilization coating agent as claimed in claim 1, is characterized in that, described Black Box Tracing extract
Extracting method is: Black Box Tracing fruit and/or blade drying and crushing cross 100 mesh sieves, returns with without heat at 70% ethanol 65 DEG C
Stream extract 3 times, each 2h, solid-liquid ratio is 1:10, united extraction liquid concentrating under reduced pressure become extractum shape to constant weight, complete with acetone water 1:1
After CL, then extract with equal-volume petroleum ether, chloroform, ethyl acetate, n-butyl alcohol successively, extract concentrating under reduced pressure is become
Extractum, to constant weight, is dried and crushed into extract powder.
4. being applied in germination box by plant seed germination box sterilization coating agent described in claim 1, it is characterized in that, step is such as
Under: dopamine hydrochloride and pulullan polysaccharide are first dissolved in appropriate amounts of sterilized water, and wiring solution-forming is brushed or dip-coating obtains at germination box inwall
To primer coating；Then stone perfume (or spice) extract, Black Box Tracing extract are dissolved in 95% ethanol, brush or dip-coating is being sent out
Bud box inwall obtains inter coat, the most again brushes or dip-coating obtains face coat.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155551A (en) * | 2017-04-22 | 2017-09-15 | 枞阳县横山生态农业有限公司 | A kind of seed propagation method of jonquil |
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| CN103783093A (en) * | 2014-03-13 | 2014-05-14 | 四川龙蟒福生科技有限责任公司 | Composition having functions of sterilization and nematode prevention and control |
| CN104524615A (en) * | 2014-12-11 | 2015-04-22 | 东莞市日友汽车用品有限公司 | Air freshener and preparation method thereof |
| CN105767029A (en) * | 2014-12-22 | 2016-07-20 | 重庆市万盛经开区黑山镇供销合作社有限公司 | Chinese herbal disinfector formulation |
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| CN103783093A (en) * | 2014-03-13 | 2014-05-14 | 四川龙蟒福生科技有限责任公司 | Composition having functions of sterilization and nematode prevention and control |
| CN104524615A (en) * | 2014-12-11 | 2015-04-22 | 东莞市日友汽车用品有限公司 | Air freshener and preparation method thereof |
| CN105767029A (en) * | 2014-12-22 | 2016-07-20 | 重庆市万盛经开区黑山镇供销合作社有限公司 | Chinese herbal disinfector formulation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107155551A (en) * | 2017-04-22 | 2017-09-15 | 枞阳县横山生态农业有限公司 | A kind of seed propagation method of jonquil |
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| CN106070359B (en) | 2018-09-14 |
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