CN105053015A - Biopesticide for controlling cercospora varrireolorWinter, and preparation method for biopesticide - Google Patents
Biopesticide for controlling cercospora varrireolorWinter, and preparation method for biopesticide Download PDFInfo
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- CN105053015A CN105053015A CN201510412155.0A CN201510412155A CN105053015A CN 105053015 A CN105053015 A CN 105053015A CN 201510412155 A CN201510412155 A CN 201510412155A CN 105053015 A CN105053015 A CN 105053015A
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- biopesticide
- bacillus subtilis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the field of biotechnology, in particular to a biopesticide for controlling cercospora varrireolorWinter, and a preparation method for the biopesticide. Through the compounding of bacillus subtilis HS5B5 which has the antagonism to pathogenic fungi of cercospora varrireolorWinter, biological fertilizer and a Bordeaux mixture, growth and reproduction of cladosporium paeoniae can be effectively inhibited, so that the purpose of cercospora varrireolorWinter control can be realized; meanwhile, the Bordeaux mixture also has the inhibiting effect on other fungus diseases of peony, so that the control effect on the fungus diseases of peony is improved after the Bordeaux mixture is compounded with bacillus subtilis and the biological fertilizer. According to the biopesticide, as the biological fertilizer and the Bordeaux mixture are added into the bacillus subtilis HS5B5, the florescence is prolonged by 2-3 days averagely as compared with that of conventional planting. The biopesticide is adopted to control cercospora varrireolorWinter, and is safe to people and animals, free of environment pollution, efficient and broad in spectrum, and the possibility of drug resistance generation is low.
Description
Technical field
The invention belongs to biological technical field, being specifically related to a kind of biopesticide for preventing and treating tree peony brown spot and preparation method thereof.
Background technology
Tree peony is the famous ornamental flower of China, is growing and in During Overwintering Period, is easily being subject to the dip-dye of pathogenic microorganism and causes pathology.Peony disease species survey is many, and harm is serious, and these disease great majority are fungal diseases, such as brown spot, gray mold, rust, powdery mildew and violet root rot etc.They can cause peony leaves local necrosis or whole leaf withered, the harm leaf of tree peony, branch and rhizome portion, make tree peony fall leaves in advance, affect it and nourish and grow and ornamental value.Tree peony brown spot is one of the most serious disease, and main harm blade, also can infect stem and flower.Disease is many to be occurred before flowering afterwards, continues up to the dead leaf phase.Disease fungus be tree peony branch spore mould (
cladosporiumpaeoniae), belong to Deuteromycotina, Haplomycetes, Moniliales, cladosporium belongs to.Cause of disease is survived the winter in dead leaf or soil with mycelium or conidium, and propagate early summer by wind and rain and start to occur, autumn, harm was serious, and high temperature, high humidity, illumination deficiency, improper ventilation, continuous cropping etc. are all conducive to disease and occur.
The control of tree peony brown spot mainly relies on the use of chemicals now, and this is not only harmful to health and ecological environment security, and easily causes pathogenic microorganism pesticide resistance.Biopesticide utilizes microorganism or its metabolite to control diseases and pests of agronomic crop or regulates the prevention and controls of crop growth, but its investigation and application in the control of tree peony fungal disease is also little.In order to strengthen the fast development of tree peony industry, develop health and ecological environment security is harmless, not easily cause the drug-fast biopesticide of cause of disease extremely urgent, bacillus subtilis HS5B5 has obvious inhibition by Ant agonism to the mould tree peony brown spot for disease fungus of tree peony branch spore, to other fungal diseases as tree peony powdery mildew also has certain inhibition but DeGrain, and bio-fertilizer and bordeaux mixture are having positive effect by microbial nutrition competition and chemical action in the suppression of tree peony powdery mildew and plant growth-promoting, kind of blooming period prolonging.
Summary of the invention
The object of this invention is to provide a kind of biopesticide for preventing and treating tree peony brown spot and preparation method thereof, can not only effectively preventing tree peony brown spot, environmental sound, and can effectively promote growing of tree peony, kind of blooming period prolonging, prevents and treats some other Common fungi disease.
The technical solution used in the present invention is: a kind of biopesticide for preventing and treating tree peony brown spot, described biopesticide is made up of bacillus subtilis, bio-fertilizer and bordeaux mixture, and preparation process each composition weight number used is respectively: bacillus subtilis 1-2 part, bio-fertilizer 8-10 part and bordeaux mixture 4-5 part, described bacillus subtilis called after bacillus subtilis (
bacillussubtilis) HS5B5, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, preserving number is: CGMCCNo.6088.
The preparation method of described biopesticide, comprises the following steps:
The preparation of step one, described bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, fermenting cellar fermentation is put into after stirring, take out after 6-7 days, obtained mixture, add colza cake, and the mass ratio of mixture and colza cake is 2:1 in mixture, airing after stirring, obtained bio-fertilizer;
The preparation of step 2, described bordeaux mixture
Copper sulphate is put into dilution with water, and the mass ratio of copper sulphate and water is 1:80, after stirring, obtained dilute sulphuric acid copper, puts into water by quicklime, and the mass ratio of quicklime and water is 1:40, after stirring, obtained milk of lime, pours in milk of lime by dilute sulphuric acid copper, limit bevelling stirs, obtained bordeaux mixture;
Step 3, described bacillus subtilis HS5B5, bio-fertilizer and bordeaux mixture are mixed after to stir i.e. obtained biopesticide, and in biopesticide, the parts by weight of each composition are the parts by weight of bacillus subtilis HS5B5 is 1-2 part, the parts by weight of bio-fertilizer are 8-10 part, and the parts by weight of bordeaux mixture are 4-5 part.
The present invention is by using the bordeaux mixture of autogamy, and there is extremely low inhibitory action to the gemma of the bacillus subtilis HS5B5 in biopesticide, survival rate is 92-95%.
Bacillus subtilis in the present invention (
bacillussubtilis) HS5B5, on May 9th, 2012 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, deposit number: CGMCCNo.6088.Saccharomycete in the present invention (
trichosporoncapitatum) buy in source, Shaanxi one bio tech ltd, this culture presevation is in China typical culture collection center, and preservation bacterium number is CCTCCAY93140.Tree peony branch spore in experimentation of the present invention mould (
cladosporiumpaeoniae) purchased from Chinese agriculture Microbiological Culture Collection administrative center, bacterial strain preserving number is ACCC36063.
Beneficial effect
The present invention adopt to the mould bacillus subtilis HS5B5 of tree peony brown spot disease fungus tree peony branch spore and bio-fertilizer and bordeaux mixture composite, more effectively can suppress the growth and breeding that tree peony branch spore is mould, and then reach the object of control tree peony brown spot, bordeaux mixture has inhibitory action to tree peony powdery mildew simultaneously, by with bacillus subtilis and bio-fertilizer composite after, enhance the control efficiency to tree peony powdery mildew.The present invention adds bio-fertilizer and bordeaux mixture in bacillus subtilis HS5B5, can also kind of blooming period prolonging, on average compared with general planting extends 2-3 days.The method that the present invention adopts biopesticide to prevent and treat, to person poultry safety, environmentally safe, high-efficiency broad spectrum, not easily develops immunity to drugs.
Embodiment
A kind of biopesticide for preventing and treating tree peony brown spot, described biopesticide is made up of bacillus subtilis, bio-fertilizer and bordeaux mixture, and preparation process each composition weight number used is respectively: bacillus subtilis 1-2 part, bio-fertilizer 8-10 part and bordeaux mixture 4-5 part, described bacillus subtilis is bacillus subtilis HS5B5.
The preparation method of described biopesticide, comprises the following steps:
The preparation of step one, described bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, fermenting cellar fermentation is put into after stirring, take out after 6-7 days, obtained mixture, add colza cake, and the mass ratio of mixture and colza cake is 2:1 in mixture, airing after stirring, obtained bio-fertilizer;
The preparation of step 2, described bordeaux mixture
Copper sulphate is put into dilution with water, and the mass ratio of copper sulphate and water is 1:80, after stirring, obtained dilute sulphuric acid copper, puts into water by quicklime, and the mass ratio of quicklime and water is 1:40, after stirring, obtained milk of lime, pours in milk of lime by dilute sulphuric acid copper, limit bevelling stirs, obtained bordeaux mixture;
Step 3, described bacillus subtilis HS5B5, bio-fertilizer and bordeaux mixture are mixed after to stir i.e. obtained biopesticide, and in biopesticide, the parts by weight of each composition are the parts by weight of bacillus subtilis HS5B5 is 1-2 part, the parts by weight of bio-fertilizer are 8-10 part, and the parts by weight of bordeaux mixture are 4-5 part.
Embodiment 1
A kind of biopesticide for preventing and treating tree peony brown spot, described biopesticide is made up of bacillus subtilis, bio-fertilizer and bordeaux mixture, and preparation process each composition weight number used is respectively: bacillus subtilis 2 parts, bio-fertilizer 10 parts and bordeaux mixture 5 parts, described bacillus subtilis called after bacillus subtilis HS5B5.
The preparation method of described biopesticide, comprises the following steps:
The preparation of step one, described bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, fermenting cellar fermentation is put into after stirring, take out after 7 days, obtained mixture, add colza cake, and the mass ratio of mixture and colza cake is 2:1 in mixture, airing after stirring, obtained bio-fertilizer;
The preparation of step 2, described bordeaux mixture
Copper sulphate is put into dilution with water, and the mass ratio of copper sulphate and water is 1:80, after stirring, obtained dilute sulphuric acid copper, puts into water by quicklime, and the mass ratio of quicklime and water is 1:40, after stirring, obtained milk of lime, pours in milk of lime by dilute sulphuric acid copper, limit bevelling stirs, obtained bordeaux mixture;
Step 3, described bacillus subtilis HS5B5, bio-fertilizer and bordeaux mixture are mixed after to stir i.e. obtained biopesticide, and in biopesticide, the parts by weight of each composition are the parts by weight of bacillus subtilis HS5B5 is 2 parts, the parts by weight of bio-fertilizer are 10 parts, and the parts by weight of bordeaux mixture are 5 parts.
Embodiment 2
A kind of biopesticide for preventing and treating tree peony brown spot, described biopesticide is made up of bacillus subtilis, bio-fertilizer and bordeaux mixture, and preparation process each composition weight number used is respectively: bacillus subtilis 1 part, bio-fertilizer 8 parts and bordeaux mixture 4 parts, described bacillus subtilis called after bacillus subtilis HS5B5.
The preparation method of described biopesticide, comprises the following steps:
The preparation of step one, described bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, fermenting cellar fermentation is put into after stirring, take out after 6 days, obtained mixture, add colza cake, and the mass ratio of mixture and colza cake is 2:1 in mixture, airing after stirring, obtained bio-fertilizer;
The preparation of step 2, described bordeaux mixture
Copper sulphate is put into dilution with water, and the mass ratio of copper sulphate and water is 1:80, after stirring, obtained dilute sulphuric acid copper, puts into water by quicklime, and the mass ratio of quicklime and water is 1:40, after stirring, obtained milk of lime, pours in milk of lime by dilute sulphuric acid copper, limit bevelling stirs, obtained bordeaux mixture;
Step 3, described bacillus subtilis HS5B5, bio-fertilizer and bordeaux mixture are mixed after to stir i.e. obtained biopesticide, and in biopesticide, the parts by weight of each composition are the parts by weight of bacillus subtilis HS5B5 is 1 part, the parts by weight of bio-fertilizer are 8 parts, and the parts by weight of bordeaux mixture are 4 parts.
Test one
On April 6th, 2015, tree peony, as subjects, is divided into A, B, C, D tetra-groups by the 400 strain tree peonies choosing international Peony growing district, peony garden, Luoyang, and mould to the tree peony branch spore of the tree peony seedling inoculation equivalent in A, B, C, D tetra-.A group uses biopesticide of the present invention, sprays once every two weeks; B group uses the bacillus subtilis HS5B5 bacterium liquid in the present invention, sprays once every two weeks; Biopesticide after C group uses the present invention to remove bacillus subtilis HS5B5, sprayed once every two weeks; D group sprayed a water every two weeks, in contrast.
Daily inspection in June 5 result in 2015: occur without tree peony brown spot and tree peony powdery mildew in A group, plant strain growth is healthy and strong, B group has 6 strain tree peony generation brown spots, has 17 strain tree peony generation powdery mildews, and plant is obviously be not as healthy and strong as A group, 32 strain tree peony generation brown spots in C group, 2 strain tree peony generation powdery mildews, though plant is healthy and strong not as A group obviously healthy and strong than B group, 79 strain tree peony generation brown spots in D group, 14 strain tree peony generation powdery mildews simultaneously, plant strain growth is small and weak.
Result shows: bacillus subtilis HS5B5 can prevent tree peony brown spot, also there is certain inhibitory action to tree peony powdery mildew simultaneously, bio-fertilizer and bordeaux mixture is used in combination can increase soil fertility, stimulating plant grows, promote that tree peony is to the absorption of nutrient, though have certain effect but DeGrain to prevention tree peony brown spot and tree peony powdery mildew, and biopesticide of the present invention more effectively can not only prevent tree peony brown spot, and can effectively prevent tree peony powdery mildew, the growth of plant can also be promoted simultaneously.
Test two
Choose the tree peony that 300 strain growing ways are identical, be divided into A, B, C tri-groups, wherein A group uses biopesticide of the present invention, and B group uses the bio-fertilizer in the present invention, and C group does not add any composition.Observe and record growing state.
Result of the test: the A group average whole florescence is 11 days, at B group average 9 days whole florescences, the C group average whole florescence is 6 days.
Result shows: bio-fertilizer can play and extend the effect of peony forescence, add bacillus subtilis HS5B5 and bordeaux mixture form biopesticide of the present invention after more obvious to the prolongation effect of peony forescence.
The result of test one and test two shows, bacillus subtilis HS5B5 leads to has obvious inhibition to the mould tree peony brown spot for disease fungus of tree peony branch spore, also have certain inhibition to tree peony powdery mildew, bio-fertilizer and bordeaux mixture have positive effect in the suppression of tree peony powdery mildew and plant growth-promoting, kind of blooming period prolonging.After passing ratio mixing, biopesticide of the present invention can suppress the activity of tree peony brown spot and tree peony powdery mildew disease fungus, increase premunition and the resistance of tree peony, promote plant strain growth, its effect be not only bacillus subtilis, bio-fertilizer and bordeaux mixture simple cumulative, also have obvious synergistic function between composite rear each composition.
Claims (1)
1. one kind for preventing and treating the biopesticide of tree peony brown spot, it is characterized in that: described biopesticide is made up of bacillus subtilis, bio-fertilizer and bordeaux mixture, and preparation process each composition weight number used is respectively: bacillus subtilis 1-2 part, bio-fertilizer 8-10 part and bordeaux mixture 4-5 part, described bacillus subtilis called after bacillus subtilis (
bacillussubtilis) HS5B5, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, and preserving number is: CGMCCNo.6088;
The preparation method of described biopesticide, comprises the following steps:
The preparation of step one, described bio-fertilizer
Saccharomycete is added in excrement of animals, and the ratio of excrement of animals and saccharomycete quality is 500:1, fermenting cellar fermentation is put into after stirring, take out after 6-7 days, obtained mixture, add colza cake, and the mass ratio of mixture and colza cake is 2:1 in mixture, airing after stirring, obtained bio-fertilizer;
The preparation of step 2, described bordeaux mixture
Copper sulphate is put into dilution with water, and the mass ratio of copper sulphate and water is 1:80, after stirring, obtained dilute sulphuric acid copper, puts into water by quicklime, and the mass ratio of quicklime and water is 1:40, after stirring, obtained milk of lime, pours in milk of lime by dilute sulphuric acid copper, limit bevelling stirs, obtained bordeaux mixture;
Step 3, described bacillus subtilis HS5B5, bio-fertilizer and bordeaux mixture are mixed after to stir i.e. obtained biopesticide, and in biopesticide, the parts by weight of each composition are the parts by weight of bacillus subtilis HS5B5 is 1-2 part, the parts by weight of bio-fertilizer are 8-10 part, and the parts by weight of bordeaux mixture are 4-5 part.
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Cited By (2)
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| CN106116883A (en) * | 2016-06-27 | 2016-11-16 | 河南科技大学 | A kind of preparation method of Paeonia suffruticosa pouring root composite microbic bacterial fertilizer |
| CN108315281A (en) * | 2018-04-17 | 2018-07-24 | 山东省林业科学研究院 | One bacillus subtilis and its application in preventing Red Spot Disease of Peony Tree |
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