CN105053021B - For preventing the preparation of rice water weevil and preparation method - Google Patents
For preventing the preparation of rice water weevil and preparation method Download PDFInfo
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- CN105053021B CN105053021B CN201510487856.0A CN201510487856A CN105053021B CN 105053021 B CN105053021 B CN 105053021B CN 201510487856 A CN201510487856 A CN 201510487856A CN 105053021 B CN105053021 B CN 105053021B
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- rice
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- rhabditis axei
- water weevil
- rice water
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Abstract
The present invention relates to the control method for invading root pest, applications of more particularly to rhabditis axei R. (Oscheius) chongmingensisTumian in rice water weevil prevention.Preparation is made in rhabditis axei R. (Oscheius) chongmingensisTumian by the present inventor, it is applied in rice terrace, the result shows that the significant effect of the control of nematode rice water weevil, larva effect is prevented up to more than 75% (correction effective percentage 75.76%), Revision insect recluced rate is up to more than 90% (92.67%).
Description
Technical field
The present invention relates to a kind of technical field of biological control, and rhabditis axei Rhabditis is utilized more particularly to a kind of
(Oscheius) preparation of chongmingensisTumian preventions rice water weevil and its application and preparation method.
Background technology
Rice water weevil (LissorhoptrusoryzophilusKuschel) originates in U.S. Mississippi Valley, belongs to
Coleoptera (Coleoptera) Culculionidae (Curculionidae).Its single-female generation type was passed to Asian countries in 1976 for the first time
Japan, 1988 for the first time be passed to China Tanghai County, Hebei Province, hereafter gradually Shaanxi, Jiangsu, Fujian, Jiangxi, Yunnan, Guangdong and
The ground such as Guangxi continue to spread, and harm aggravates, and occurring area is more than 400,000 hm2.Rice water weevil main harm rice and with corn, sweet
Sugarcane, wheat, barley, herbage, grassy weed etc. are host, can be propagated, had by straw, paddy, rice husk and the vehicles
The features such as harm time is long, and breeding spread speed is fast, drug resistance and strong resistance.Rice water weevil adult gnaws rice leaf, larva moth
Rice root is eaten, typically results in the rice underproduction 15%~20%, serious more than 50% underproduction, or even total crop failure, is the important inspection of rice
Epidemic disease pest.Economic loss caused by rice water weevil not only shows that paddy loses directly caused by harm, is also embodied by preventing
The increase of expense.
Adult overwintering survival rate is reduced mainly by winter Soil tillage for the prevention of this class pest for a long time, quarantine is sealed
Lock control anthropochory and rice terrace sprinkling high concentration, high toxicity chemical pesticide.But these control effects are unsatisfactory, and right
Dispenser personnel and ecological environment toxicity are larger.
Rhabditis (Oscheius) sp is resourceful, widely distributed, is easily obtained and cultivates.Conventionally small bar belongs to Rhabditis
(Oscheius) nematode is food bacterium class nematode, so far both at home and abroad there is not yet with rhabditis axei R. (Oscheius)
ChongmingensisTumian prevents the report of rice water weevil, more without the report of field control effect.
Invention content
The present invention is directed to the blank in above-mentioned field, provides rhabditis axei R. (Oscheius) chongmingensisTumian
Application in rice water weevil is prevented to solve the problems, such as in production, widens biological control approach.
Applications of rhabditis axei R. (Oscheius) chongmingensisTumian in rice grub is prevented.
The rice grub is rice water weevil.
The application is that the rhabditis axei preparation is imposed on to the plant endangered by rice water weevil in the growth and development of plants phase
Root system water and Soil Interface.
The plant is rice.
The prevention is to kill Larva of Rice Water Weevil.
The rhabditis axei preparation is rhabditis axei moisturizer constituent, and a concentration of 10000IJs/ml, amount of application is
2500-40000IJs/ plants of rice.
A kind of biological pesticide for preventing rice water weevil, which is characterized in that its active ingredient is rhabditis axei R.
(Oscheius)chongmingensisTumian。
Above-mentioned biological pesticide, is the moisturizer constituent of the rhabditis axei, a concentration of 10000IJs/ml.
The preparation method of the biological pesticide of above-mentioned prevention rice water weevil, includes the following steps：By the rhabditis axei R.
(Oscheius) chongmingensisTumian is packaged in the packing material for being labeled with prevention rice water weevil.
The source of biomaterial and record source
Rhabditis axei R. (Oscheius) chongmingensisTumian is recorded in Liu Qizhi, Zden ˇ ek
M.Zhang Lijuan,VladimírP.and Dong Lamei.2012.Re-description of
Oscheiuschongmingensis(Zhang etal.,2008)(Nematoda:Rhabditidae)and its
entomopathogenicity.Nematology,14(2):139-149. in a text；
The rhabditis axei that the present invention uses is to acquire, detach from the Cotton Soil of Inner Mongol, purifying, expanding numerous gained, in application
There is preservation in people laboratory, can external disclosure granting.
Inventor has found that rhabditis axei R. (Oscheius) chongmingensisTumian have prevention to rice water weevil
Activity, based on the discovery, the present invention provides a kind of new entomopathogenic nematode preparation and application thereof, using patent first of the present invention
Applicant collected rhabditis axei R. (Oscheius) chongmingensisTumian from the Cotton Soil of Inner Mongol is made
Agent is applied in rice terrace, the results showed that the significant effect of the control of nematode Larva of Rice Water Weevil prevents larva effect up to 75%
(correction effective percentage 75.76%) above.
Based on the present invention, rhabditis axei R. (Oscheius) chongmingensisTumian can be prepared into biological agriculture
Medicine is used to prevent rice water weevil.Therefore, the present invention is claimed preparation method to the biological pesticide, it is any using commercial distribution as
The process that purpose prepares the biological pesticide belongs to the claimed range of the present invention, and preparing the process of the biological pesticide, refer to will be small
Rhabditida R. (Oscheius) chongmingensisTumian is packaged in the packing material for being labeled with prevention rice water weevil.
Description of the drawings
Fig. 1 is each dosage (concentration) rhabditis axei R. (Oscheius) chongmingensisTumian to rice water weevil children
The correction of worm is efficient.
Specific embodiment
The present invention is described in further detail With reference to embodiment, but is not intended to limit the model of the present invention
It encloses.Unless otherwise specified, the operation used in following embodiments is conventional method, and used reagent commercially available can obtain
.
The source of biomaterial and record source
Rhabditis axei R. (Oscheius) chongmingensisTumian is recorded in Liu Qizhi, Zden ˇ ek
M.Zhang Lijuan,VladimírP.and Dong Lamei.2012.Re-description of
Oscheiuschongmingensis(Zhang etal.,2008)(Nematoda:Rhabditidae)and its
entomopathogenicity.Nematology,14(2):139-149. in a text；
The rhabditis axei that the present invention uses is to acquire, detach from the Cotton Soil of Inner Mongol, purifying, expanding numerous gained, in application
There is preservation in people laboratory, can external disclosure granting.
The field effect of embodiment one, rhabditis axei R. (Oscheius) chongmingensisTumian prevention rice water weevils
Fruit case
1 materials and methods
1.1 test material
Reagent agent：Rhabditis axei after collection is suspended in aqueous solution, in 4 DEG C of preservations, is applied in 20 days.Nematode is applied
With for example following test methods of concentration.
1.2 test method
In May, 2015 chooses the aggrieved serious water of one mu of rice water weevil in Guangxi province Guilin City Quanzhou County Quanzhou town July 1st village
Rice field, without other preventions.Experimental plot, experimental plot 0.5m are divided in this mu of rice terrace2(there are about 20 plants of rice).
Rhabditis axei R. (Oscheius) chongmingensisTumian is made into the insect of a concentration of 10000IJs/mL
Nematode moisturizer constituent, according to 1 × 105IJs/m2、2×105IJs/m2、4×105IJs/m2、8×105IJs/m2、16×
105IJs/m2(i.e. 2.5 × 103The strain of IJs/ rice, 5 × 103The strain of IJs/ rice, 10 × 103The strain of IJs/ rice, 20 × 103The strain of IJs/ rice, 40
×103The strain of IJs/ rice) five concentration gradients apply Insectivorous nematode to the rice strain root of experimental plot.Method of administration：Needed for taking-up
Itself and 5 liters of water are packed into 16L backpack Insecticide sprayer mixings, are equably sprayed on by the Insectivorous nematode moisturizer constituent of amount of application
0.25 liter of nematode liquid is sprayed around the root damage area of every plant of rice in corresponding experimental plot, averagely every plant of rice root.
Equivalent clear water is applied in each concentration processing 3 repetitions (i.e. 3 cells) of setting, control, and remaining area is set as crop field control, into
Row Routine Management.It extracts with root 3 plants of rice in each cell respectively within 7th day, the 18th day, observes and records every plant of rice root
Larva of Rice Water Weevil survival number.
1.3 computational methods
It is calculated by following equation：
Revision insect recluced rate %=(insect population number after before processing insect population number-processing)/before processing insect population number × 100%
Correct effective percentage %=(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(1-check plot insect population decline
Rate) × 100%
2 results and analysis
The Revision insect recluced rate of 2.1 rhabditis axei Tumian field control Larva of Rice Water Weevil
As seen from Table 1：It is young to rice water weevil for rhabditis axei R. (Oscheius) chongmingensisTumian of examination
Worm has a very high infecting potential, 5 kinds of dosage of application after 18 days Revision insect recluced rate between 60-93%.Wherein, administration dosage is
4×105IJs/m2(i.e. 10 × 103IJs/ rice strain) when control effect it is best, nearly 84% (83.78%) of Revision insect recluced rate after 7 days,
Nearly 93% (92.67%) of Revision insect recluced rate after 18 days.
Table 1. rhabditis axei R. (Oscheius) chongmingensisTumian prevents the Revision insect recluced rate of rice water weevil
The correction of 2.2 rhabditis axei Tumian field control Larva of Rice Water Weevil is efficient
As can be seen from Figure 1, rice water weevil children is infected for examination rhabditis axei R. (Oscheius) chongmingensisTumian
Worm the 18th day, correction effective percentage is 4 × 105IJs/m2(i.e. 10 × 103The strain of IJs/ rice) it is best under nematode dosage, it reaches
75.76%；From the point of view of overall trend, the excessively high too low control effect of application concentration is bad.
The present invention relates to rhabditis axei R. (O.) chongmingensisTumian (abbreviation Tumian) preventions to invade root pest
In application.Rhabditis axei Tumian is made preparation and is applied in rice terrace by the present inventor, the results showed that rhabditis axei
Tumian prevents the significant effect of rice water weevil, and prevention larva effect is up to 75% more than (correction effective percentage 75.76%).
Claims (2)
1. rhabditis axei R. (Oscheius) chongmingensisTumian invade answering in root pest rice water weevil in prevention
With,
The application includes rhabditis axei rhabditis axei preparation is made, and the rhabditis axei preparation is applied to plant
Growing environment in,
The plant is rice,
The rhabditis axei preparation is rhabditis axei moisturizer constituent, a concentration of 10000IJs/ml, amount of application for 1 ×
105IJs/m2-16×105IJs/m2。
2. application according to claim 1, the application is to apply the rhabditis axei preparation in the growth and development of plants phase
In the root system of plant water endangered by rice water weevil, in Soil Interface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510487856.0A CN105053021B (en) | 2015-08-10 | 2015-08-10 | For preventing the preparation of rice water weevil and preparation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510487856.0A CN105053021B (en) | 2015-08-10 | 2015-08-10 | For preventing the preparation of rice water weevil and preparation method |
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| Publication Number | Publication Date |
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| CN105053021A CN105053021A (en) | 2015-11-18 |
| CN105053021B true CN105053021B (en) | 2018-06-26 |
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Families Citing this family (1)
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| CN111990138B (en) * | 2020-09-09 | 2022-05-06 | 湖南省水稻研究所 | Method for reducing rice field pest base number by using entomopathogenic nematodes |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222023A (en) * | 2014-08-30 | 2014-12-24 | 北京安和亿泰生物工程技术有限公司 | Steinernema batch breeding and culture method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| AU2009222557B2 (en) * | 2009-06-17 | 2015-06-11 | Monash University | Modified arthropod and method of use |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222023A (en) * | 2014-08-30 | 2014-12-24 | 北京安和亿泰生物工程技术有限公司 | Steinernema batch breeding and culture method |
Non-Patent Citations (2)
| Title |
|---|
| 小杆线虫Rhabditis sp.品系Tumian的初步鉴定、生物学及应用评价;曹海锋;《中国农业大学硕士学位论文》;20070723;10、43 * |
| 稻水象甲生物学及药剂防治初步研究;凌云;《作物研究》;20091130;83 * |
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