CN105053021A - Preparation used for prevention and control of LissorhoptrusoryzophilusKuschel and preparation method - Google Patents
Preparation used for prevention and control of LissorhoptrusoryzophilusKuschel and preparation method Download PDFInfo
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- CN105053021A CN105053021A CN201510487856.0A CN201510487856A CN105053021A CN 105053021 A CN105053021 A CN 105053021A CN 201510487856 A CN201510487856 A CN 201510487856A CN 105053021 A CN105053021 A CN 105053021A
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- oscheius
- chongmingensistumian
- rice
- preparation
- rhabditis
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Abstract
The invention relates to a prevention and control method for root-attacking pests, specifically to application of Rhabditis (Oscheius) chongmingensisTumian in the prevention and control of LissorhoptrusoryzophilusKuschel. Accordingn to the invention, Rhabditis (Oscheius) chongmingensisTumian is prepared into preparation which is applied in a paddy field. Results show that the prevention and control of the Rhabditis (Oscheius) chongmingensisTumian to the LissorhoptrusoryzophilusKuschel has significant effects, wherein prevention and treatment rate of LissorhoptrusoryzophilusKuschel reaches more than 75% (correction rate reaches 75.76%), and population decline rate of the LissorhoptrusoryzophilusKuschel reaches more than 90% (92.67%).
Description
Technical field
The present invention relates to a kind of technical field of biological control, particularly relate to one and utilize rhabditis axei Rhabditis (Oscheius) chongmingensisTumian to prevent and treat preparation and the applications and preparation method thereof of rice water weevil.
Background technology
Rice water weevil (LissorhoptrusoryzophilusKuschel) originates in U.S. Mississippi Valley, belongs to coleoptera (Coleoptera) Culculionidae (Curculionidae).Its parthenogenesis type imported Japan of Asian countries into first in 1976, within 1988, import China Tanghai County, Hebei Province first into, after this gradually in Shaanxi, Jiangsu, Fujian, Jiangxi, Yunnan, the ground such as Guangdong and Guangxi continues to spread, harm increases the weight of, and occurring area is more than 400,000 hm
2.Rice water weevil main harm paddy rice with corn, sugarcane, wheat, barley, herbage, grassy weed etc. for host, propagate by straw, paddy, rice husk and the vehicles, have harm time long, breeding spread speed is fast, the feature such as pesticide resistance and strong stress resistance.Rice water weevil adult gnaws rice leaf, and larva moth food rice root, generally cause the paddy rice underproduction 15% ~ 20%, the serious underproduction more than 50%, even has no harvest, and is the important quarantine pest of paddy rice.The economic loss that rice water weevil causes not only shows the paddy loss directly endangering and cause, and also shows as the increase of expenses for prevention and control.
Control for a long time for this class pest mainly reduces adult overwintering survival rate by Soil tillage in winter, control anthropochory is blocked in quarantine and paddy field sprays high concentration, high toxicity chemical pesticide.But these control efficiency are unsatisfactory, and to dispenser personnel and ecotope toxicity larger.
Rhabditis (Oscheius) sp aboundresources, widely distributed, be easy to obtain and cultivate.Conventionally little bar belongs to Rhabditis (Oscheius) nematode is food bacterium class nematode, there is not yet the report preventing and treating rice water weevil with this rhabditis axei R. (Oscheius) chongmingensisTumian up to now both at home and abroad, more there is no the report of field control effect.
Summary of the invention
The present invention is directed to the blank in above-mentioned field, the application of rhabditis axei R. (Oscheius) chongmingensisTumian in control rice water weevil is provided, to solve the difficult problem in production, widens biological control approach.
The application of rhabditis axei R. (Oscheius) chongmingensisTumian in water prevention rice pest.
Described rice grub is rice water weevil.
Described application is that described rhabditis axei preparation is imposed on the root system of plant water and Soil Interface that are endangered by rice water weevil at grow up develop of plant.
Described plant is paddy rice.
Larva of Rice Water Weevil is killed in described control.
Described rhabditis axei preparation is rhabditis axei humectant constituent, and concentration is 10000IJs/ml, and amount of application is 2500-40000IJs/ strain paddy rice.
Prevent and treat a biopesticide for rice water weevil, it is characterized in that, its active ingredient is rhabditis axei R. (Oscheius) chongmingensisTumian.
Above-mentioned biopesticide, be described rhabditic humectant constituent, concentration is 10000IJs/ml.
The preparation method of the biopesticide of above-mentioned control rice water weevil, comprises the steps: described rhabditis axei R. (Oscheius) chongmingensisTumian to be packaged in the packing material being labeled with control rice water weevil.
the source of biomaterial and record source
Rhabditis axei R. (Oscheius) chongmingensisTumian is recorded in LiuQizhi, Zden ˇ ekM.ZhangLijuan, Vladim í rP.andDongLamei.2012.Re-descriptionofOscheiuschongmingen sis (Zhangetal., 2008) (Nematoda:Rhabditidae) anditsentomopathogenicity.Nematology, in 14 (2): 139-149. mono-literary compositions;
The rhabditis axei that the present invention uses is collection from the Cotton Soil of Inner Mongol, separation, purifying, the numerous gained of expansion, has preservation in applicant laboratory, can external disclosure granting.
It is active that inventor finds that rhabditis axei R. (Oscheius) chongmingensisTumian has control to rice water weevil, based on this discovery, the invention provides a kind of new entomopathogenic nematode preparation and uses thereof, rhabditis axei R. (Oscheius) chongmingensisTumian adopting patent first applicant of the present invention to collect from the Cotton Soil of Inner Mongol makes preparation, be applied in paddy field, result shows the Be very effective of this nematoda control Larva of Rice Water Weevil, and control larva effect reaches more than 75% (correcting efficient 75.76%).
Based on the present invention, rhabditis axei R. (Oscheius) chongmingensisTumian can be prepared into biopesticide for preventing and treating rice water weevil.Therefore; the preparation method of request protection of the present invention to this biopesticide; any process preparing this biopesticide for the purpose of commercial distribution all belongs to the scope of request of the present invention protection, and the process preparing this biopesticide refers to rhabditis axei R. (Oscheius) chongmingensisTumian to be packaged in the packing material being labeled with control rice water weevil.
Accompanying drawing explanation
Fig. 1 is that the correction of each dosage (concentration) rhabditis axei R. (Oscheius) chongmingensisTumian to Larva of Rice Water Weevil is efficient.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but do not limit the scope of the invention.If no special instructions, the operation used in following embodiment is conventional method, and the reagent adopted all can be commercially available.
the source of biomaterial and record source
Rhabditis axei R. (Oscheius) chongmingensisTumian is recorded in LiuQizhi, Zden ˇ ekM.ZhangLijuan, Vladim í rP.andDongLamei.2012.Re-descriptionofOscheiuschongmingen sis (Zhangetal., 2008) (Nematoda:Rhabditidae) anditsentomopathogenicity.Nematology, in 14 (2): 139-149. mono-literary compositions;
The rhabditis axei that the present invention uses is collection from the Cotton Soil of Inner Mongol, separation, purifying, the numerous gained of expansion, has preservation in applicant laboratory, can external disclosure granting.
Embodiment one, rhabditis axei R. (Oscheius) chongmingensisTumian prevent and treat the field efficacy case of rice water weevil
1 materials and methods
1.1 test material
Reagent agent: the rhabditis axei after collecting is suspended in aqueous, 4 DEG C of preservations, uses in 20 days.Nematode application concentration is as following test method.
1.2 test method
Choose one mu of rice water weevil in May, 2015 to be injured serious paddy field in village's July 1st, Quanzhou town, Quanzhou County, Guangxi province Guilin City, do not carry out other control.In this mu of paddy field, divide experimental plot, experimental plot is 0.5m
2(about having 20 strain paddy rice).
Rhabditis axei R. (Oscheius) chongmingensisTumian is made into the Insectivorous nematode humectant constituent that concentration is 10000IJs/mL, according to 1 × 10
5iJs/m
2, 2 × 10
5iJs/m
2, 4 × 10
5iJs/m
2, 8 × 10
5iJs/m
2, 16 × 10
5iJs/m
2(namely 2.5 × 10
3iJs/ rice strain, 5 × 10
3iJs/ rice strain, 10 × 10
3iJs/ rice strain, 20 × 10
3iJs/ rice strain, 40 × 10
3the strain of IJs/ rice) five concentration gradients use Insectivorous nematode to the rice strain root of experimental plot.Application process: the Insectivorous nematode humectant constituent taking out required amount of application, itself and 5 premium on currency are loaded 16L backpack Insecticide sprayer mix, be sprayed on the root damage area of every strain paddy rice in corresponding experimental plot equably, around average every strain rice root, spray 0.25 liter of nematode liquid.Each concentration process arranges 3 repetitions (i.e. 3 communities), and equivalent clear water is used in contrast, and remaining area is set to land for growing field crops contrast, carries out Routine Management.Within 7th day, the 18th day, extract with root 3 strain paddy rice respectively in each cell, observe and record every strain rice root Larva of Rice Water Weevil survival number.
1.3 computational methods
By following formulae discovery:
Insect population number × 100% before Revision insect recluced rate %=(before process the rear insect population number of insect population number-process)/process
Correct efficient %=(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(1 – check plot Revision insect recluced rate) × 100%
2 results and analysis
The Revision insect recluced rate of 2.1 rhabditis axei Tumian field control Larva of Rice Water Weevils
As seen from Table 1: for examination rhabditis axei R. (Oscheius) chongmingensisTumian to Larva of Rice Water Weevil, there is very high infecting potential, the 5 kinds of dosage used after 18 days Revision insect recluced rate between 60-93%.Wherein, application dosage is 4 × 10
5iJs/m
2(namely 10 × 10
3the strain of IJs/ rice) time control efficiency best, Revision insect recluced rate nearly 84% (83.78%) after 7 days, Revision insect recluced rate nearly 93% (92.67%) after 18 days.
Table 1. rhabditis axei R. (Oscheius) chongmingensisTumian prevents and treats the Revision insect recluced rate of rice water weevil
The correction of 2.2 rhabditis axei Tumian field control Larva of Rice Water Weevils is efficient
As can be seen from Figure 1, infect Larva of Rice Water Weevil the 18th day for examination rhabditis axei R. (Oscheius) chongmingensisTumian, its correction is efficient 4 × 10
5iJs/m
2(namely 10 × 10
3the strain of IJs/ rice) best under nematode dosage, reach 75.76%; From overall trend, the too high too low control efficiency of application concentration is all not good.
The present invention relates to rhabditis axei R. (O.) chongmingensisTumian (being called for short Tumian) and prevent and treat the application of invading in root insect.Rhabditis axei Tumian is made preparation and is applied in paddy field by the present inventor, and result shows that rhabditis axei Tumian prevents and treats the Be very effective of rice water weevil, and control larva effect reaches more than 75% (correcting efficient 75.76%).
Claims (8)
1. rhabditis axei R. (Oscheius) chongmingensisTumian is preventing and treating the application of invading in root insect.
2. application according to claim 1, described in invade root insect be rice water weevil.
3. application according to claim 2, described application be by described rhabditis axei preparation grow up develop of plant impose on endangered by rice water weevil root system of plant water, in Soil Interface.
4. application according to claim 3, described plant is paddy rice.
5., according to the arbitrary described application of claim 1-4, described rhabditis axei preparation is rhabditis axei humectant constituent, and concentration is 10000IJs/ml, and amount of application is 1 × 10
5iJs/m
2-16 × 10
5iJs/m
2.
6. prevent and treat a biopesticide for rice water weevil, it is characterized in that, its active ingredient is rhabditis axei R. (Oscheius) chongmingensisTumian.
7. biopesticide according to claim 6, be described rhabditic humectant constituent, concentration is 10000IJs/ml.
8. the preparation method of the biopesticide of the control rice water weevil described in claim 6 or 7, comprises the steps: described rhabditis axei R. (Oscheius) chongmingensisTumian to be packaged in the packing material being labeled with control rice water weevil.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510487856.0A CN105053021B (en) | 2015-08-10 | 2015-08-10 | For preventing the preparation of rice water weevil and preparation method |
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|---|---|---|---|
| CN201510487856.0A CN105053021B (en) | 2015-08-10 | 2015-08-10 | For preventing the preparation of rice water weevil and preparation method |
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| Publication Number | Publication Date |
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| CN105053021A true CN105053021A (en) | 2015-11-18 |
| CN105053021B CN105053021B (en) | 2018-06-26 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111990138A (en) * | 2020-09-09 | 2020-11-27 | 湖南省水稻研究所 | Method for reducing rice field pest base number by using entomopathogenic nematodes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140298501A1 (en) * | 2009-06-17 | 2014-10-02 | Monash University | Modified arthropod and method of use |
| CN104222023A (en) * | 2014-08-30 | 2014-12-24 | 北京安和亿泰生物工程技术有限公司 | Steinernema batch breeding and culture method |
-
2015
- 2015-08-10 CN CN201510487856.0A patent/CN105053021B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140298501A1 (en) * | 2009-06-17 | 2014-10-02 | Monash University | Modified arthropod and method of use |
| CN104222023A (en) * | 2014-08-30 | 2014-12-24 | 北京安和亿泰生物工程技术有限公司 | Steinernema batch breeding and culture method |
Non-Patent Citations (2)
| Title |
|---|
| 凌云: "稻水象甲生物学及药剂防治初步研究", 《作物研究》 * |
| 曹海锋: "小杆线虫Rhabditis sp.品系Tumian的初步鉴定、生物学及应用评价", 《中国农业大学硕士学位论文》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111990138A (en) * | 2020-09-09 | 2020-11-27 | 湖南省水稻研究所 | Method for reducing rice field pest base number by using entomopathogenic nematodes |
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| CN105053021B (en) | 2018-06-26 |
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