CN109662103A - A kind of composite biological pesticidal preparations for preventing and treating fruit tree ring spot - Google Patents
A kind of composite biological pesticidal preparations for preventing and treating fruit tree ring spot Download PDFInfo
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- CN109662103A CN109662103A CN201811616488.5A CN201811616488A CN109662103A CN 109662103 A CN109662103 A CN 109662103A CN 201811616488 A CN201811616488 A CN 201811616488A CN 109662103 A CN109662103 A CN 109662103A
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- biological pesticide
- tea seed
- seed cake
- fruit tree
- ring spot
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention aims to provide a kind of method can using tea seed cake as primary raw material, mix different vegetable materials according to a certain percentage, extracted after degreasing with ethanol solution, composite extract be made using the methods of filter, reduced pressure, crystallization.Product of the invention has the function of significantly inhibiting fruit tree Target spot pathogen also have strong inhibiting effect to cucumber phytophthora root rot bacterium and Monilinia fructicola.
Description
Technical field
The invention belongs to field of biological pesticide, are related to a kind of biology of compound tea seed cake prevention and treatment fruit trees ring spot such as apple and pears
Pesticidal preparations production method and application.
Background technique
Apple and pears are one of the fruit being loved by consumers in world wide, and China is that apple and pears are most in the world
Mass production state, currently, China's cultivated area of the apple and total output all occupy the first in the world.Cultivated area, yield, the export volume of pears
And kind quantity etc. is also at the forefront in the world, in China, pears are only second to apple, citrus, occupy third.
Ring spot is also tumor skin disease, scurf, be China's apple, a kind of serious disease in pears producing region, all over the world
The area Chan Li is also typical, also there is certain harm, especially high temperature, high humidity and coastal fruit to tree species such as apple, peach, Lee, apricots
Producing region is even more serious, and some time fields diseased fruit rate is up to 80% or more, is up to 70%-80%, individual orchard diseased plant rates
Influence the crucial disease of domestic planting fruit trees industry.Target spot pathogen main harm Tree Fruit, limb and blade, can especially make fruit
It rots, very big influence is produced on the quality of fruit.
Apple, black rot of pear pathogenic bacteria belong to same kind, ring spot cause of disease Perfect stage is Botryosphaeria
Dothidea, belongs to sac fungus, and Invisible element is tree shell shuttle spore bacterium (Fusicoccum aesculi).Infection of Botryosphaeria berengriana f. sp. piricola pears or apple
Fruit causes fruit wheel line decayed fruit, and Target spot pathogen (Macrophoma kuwatsukai Hara) is that the fruits industry such as apple and pears is important
The disease fungus of disease ring spot.
Disease symptom shows that limb is aggrieved mostly, and the water stain shape spot of crineous is originally formed centered on hole skin, around
Hole skin diffusion forms oblate brown dot, and rounded or oblate, central elevation is in warty, and material is hard.So
The scab further expansion grown afterwards, scab peripheral indentation, the place that infection site has a common boundary with not infected position gradually divide,
Color paleness ash to dark brown, next year generates pycnidia, black color dots grain occurs.With bark calli induction, scab
Crack occurs for surrounding protuberance, the strong intersection of disease, and scab edge tilts such as saddle, several scabs and connects together, and is formed irregular
Big spot, when serious, sick tree growing way is weak, and branch is withered.Mostly in nearly maturity period and storage period, infected position can be big for fruit morbidity
Area morbidity, infected fruit show as spot shape dot in morbidity at the beginning.There is hole skin at dot center, is just with hole skin
It is centrally formed the water stain shape spot of brown, flaring is big, and in chocolate to light brown, tool clearly crineous concentric wheel stripe waits scabs
After growth expands, entire infected position will form wheel line.Infection site rots to soften, and running down can cause full fruit to rot, hair
Acid smell out, and dark brown mucus is oozed out, gradually dehydration becomes black mummy to disease fruit, and surface is covered with black grain point.The leaf sense of fruit tree
Dye initial stage can then generate taupe scab in irregular shape, and wheel line is obvious, and scab gradually becomes grey, and meeting after a period of time
Phenomena such as scab surface grows black dot, fruit tree leaf is caused to wither and fall in advance.
For a long time, the means for preventing and treating ring spot, which mainly have, reasonably selects disease-resistant variety or disease-free nursery stock；Reinforce orchard pipe
Reason enhances tree vigo(u)r, and increasing the p application rate raises fertilizer, controls the dosage of nitrogen；Before winter and early spring rudiment, the diseases such as disease skin, sick tumor are finely struck off
Tissue；Bagging diseases prevention etc..On the basis of strengthening management, chemical control is carried out, wherein chemical pesticide is good by its control efficiency,
Quick, wide sterilization spectrum, the characteristics such as inexpensive and easy to use become a kind of most wide means of use scope.Domestic scholars are being taken turns
Line disease prevention and treatment aspect has carried out a lot of research work successively, and achieves good result.Yuan Zigeng is anti-from field management, medicament
Control and fruit storage etc. the prevention and control measure of ring rot of apple has been discussed in detail.Chi Fumei etc. is tried by indoor sterilization agent virulence
It tests and shows that 10% micro-emulsion of phenyl ether methyl cyclic-azole is best to the fungistatic effect of ring rot of apple pathogen, be secondly 43% Tebuconazole
Suspending agent.Ye Changfei etc. with lucky star missible oil (effective component is Flusilazole), (effective component is phenyl ether methyl cyclic to Difenoconazole
Azoles), Amici is up to suspending agent (effective component is Fluoxastrobin), emerald green shellfish dry suspending agent (effective component is kresoxim-methyl)), A meter Miao receives
7 kinds of medicaments such as suspending agent (effective component is benzene first Fluoxastrobin), thiophanate methyl wettable powder and carbendazol wettable powder
Prevention and treatment black rot of pear is carried out, good effect is achieved.Currently, the administrated method of domestic fruit tree ring spot generally uses and sprays wave
That more liquid, carbendazim, thiophanate-methyl, open up the chemical agents such as good fortune, Jin Nuo at Mancozeb.
Chemical pesticide, which is used for a long time, will appear that drug ingedient toxicity is big, and the residence time is long, not degradable, environmental disruption, disease
The harm such as balance that bacterium develops drug resistance, destroys plant internal and external environment.As people's awareness of environmental protection continues to increase and to food
The concern of product safety problem, and to the intensification that the problems such as pesticide residue, pathogen drug resistance recognizes, more and more researchers
Start to be dedicated to finding and develop safe and efficient biological pesticide.Zhao Baige etc. separates a kind of withered grass obtained from apple bark
Bacillus B-903 has apparent fungistatic effect in vitro ring rot of apple bacterial strain, sprays protection and fruit through field
Soaking fruit before storage processing result shows that the bacterial strain has apparent inhibiting effect to Target spot pathogen.Ji Zhaolin etc. is separated from soil and is obtained
Bacillus licheniformis W10, inhibition and efficiency test to Botryosphaeria berengeriana f. sp the result shows that, W10 bacterium solution, culturing filtrate,
Antibacterial protein all has apparent destruction or inhibiting effect to the form of germ, mycelia growth, production spore and conidial sprouting.
Antagonistic bacterium No.1 and biocontrol actinomycetes that separation screening obtains etc. also have good antagonism, Er Qiesheng to Target spot pathogen
Fungi-proofing and NaHCO3The effect prevented and treated after biocontrol microorganisms adopt ring spot can be improved in synergistic effect.Meanwhile kuh-seng alkali extract and plant
The botanical pesticides such as object endogenous metabolism object Bao Man rhzomorph also have favorable effect to ring spot.
Plant is the natural treasure-house of antibacterial substance.Really the plant antimicrobial of investment application is few in number at present, has
Tea polyphenols, allicin have very strong inhibiting effect to germ spore germination and mycelia growth, in prevention and treatment sweet potato disease, cotton in seedling stage
Disease and rape disease etc. have better effects；Carvacrol is the tool separated from Lamiaceae plant thyme aromatic oil
There is the plant-source antibacterial agent of bactericidal activity, for preventing and treating apple, peach, pears, mandarin orange bucket, peanut, the bent dew disease of cotton and fruit, potato wedge
Trichoderma disease；Soybean lecithin derives from soybean oil, can make powdery mildew gemma tubbiness, and hyphal elongation continues slowly, postpones and being formed
Conidiophore, conidium cannot also disperse propagation, and soybean lecithin can prevent and treat the powdery mildew of the vegetables such as melon, eggplant, and
Such compound is very safe to people, animal；(in vitro does not show apparent bactericidal activity to Acibenzolar, but can induce work
Object generate antibacterial immunity function) and by Secondary Metabolism of Plant generate configurations the compound with Lethal Photosensitization,
Including furocoumarin class, polyacetylene class, thiophene-based, alkaloids and quinones etc..
Plant source resistance fruit Target spot pathogen active constituent is easy to degrade because being botanical secondary metabolite after application, environment
Compatibility is good；Chemical structure with a variety of novel and uniques forms the double site even mode of action of multidigit point, is difficult to disease
Form drug resistance etc..But often there is the disadvantages of activity is lower, duration of efficacy is short, high production cost in botanical pesticide.
The technical problems to be solved by the present invention are: a kind of production method of biological pesticide compound preparation is provided, for preventing
Fruit tree ring spot is controlled, the biological pesticide of this method preparation is to be process using natural materials such as tea seed cakes as primary raw material through science,
The pesticide is nontoxic to people and animals, environmentally friendly, and alternative chemical, chemical industry synthesis pesticide used at present is widely used,
It can meet to other crops, fruit tree, vegetables, the sterilization of flowers, the demand removed the evil.
Tea seed cake is also tea bran, tea seed cake, and it is camellia oleifera fruit remaining oil-tea camellia husks blob of slag after extracting oil that particle, which is in puce,.
Tea Saponin content in tea seed cake is very high, inhibits or kill various plants disease fungus, in terms of the prevention and treatment that crops infect disease
With good effect, additionally it is possible to crop is avoided pollution caused by pesticide residue occur, improve well crop yield and
Quality.Oil tea is China one of main oilseed plant at present, the annual by-product produced after about 1,000,000 t of tea seed, oil expression
About 680,000 t of object --- Extracted From Oil-tea-cake, therefore the leftover bits and pieces of these agricultural products processing is recycled, it may have sustainable development
Ecological significance.
Summary of the invention
It is a kind of for preventing and treating the pure natural compound based on tea seed cake of fruit tree ring spot present invention aim to provide
Biological pesticide preparation and preparation method thereof application method, biological pesticide operation easy to produce, efficient, low toxicity are environmental-friendly easy
The features such as degrading, being not likely to produce drug resistance.
The vegetable materials such as tea seed cake, tealeaves, herba fibraureae recisae root or stem, Schima superba blade, Leaf of Magnolia officinalis, rule grass, ginkgo leaf are placed in baking oven 70
It DEG C dries to constant weight, then weighs tea seed cake 50-100 parts by weight, tealeaves 20-30 parts by weight, Schima superba blade 20-30 parts by weight, Cortex Magnoliae Officinalis
It is crushed after fresh leaf 7-10 parts by weight, rule grass 20-30 parts by weight, the mixing of ginkgo leaf 7-10 parts by weight, 40 meshes excessively, degreasing, then plus
Enter 70% alcohol steep 3 times, every time extraction 8-12 hours, respectively through alcohol reflux extraction, filtering, reduced pressure, the behaviour such as freeze-drying
It obtains slightly promoting powder, as compound tea seed cake biological pesticide main effect component.
Herba fibraureae recisae root or stem are ground into coarse powder, and 0.2% sulfuric acid solution is added to extract 3 times, every time extraction 8-12 hours, filter, and receive
Collect filtrate, filtrate adds 7% to saltout to obtain thick promotion powder containing salt compounded of iodine.
Compound tea seed cake biological pesticide main effect component is promoted powder with herba fibraureae recisae root or stem thickness to mix by 10: 1-5: 1,40~50
Stirred at low speed at DEG C, be mixed to prepare finished product, in it is closed, be protected from light under the conditions of save or transport.
The characteristics of compound tea seed cake biological pesticide preparation is with tea seed cake, tealeaves, herba fibraureae recisae root or stem, Schima superba blade, Leaf of Magnolia officinalis, rule
Grass, ginkgo biloba p.e are main sterilization component, and wettable powder is made.
The drug administration process for the treatment of fruit tree ring spot provided by the invention is to be watered spraying or brushing trunk, compound tea seed cake
The ratio that biological pesticide preparation is watered is 1: 200-500.The dose for brushing trunk is that compound tea seed cake biological pesticide 100g is watered 10-
30kg brushes trunk.The dosage that blade face and branch spray is the biological pesticide 5-10kg sprayed per acre after being watered dilution every time.
Technical effect
The present invention has the advantage that
1, there is efficient disease-resistance activity to the fruit trees Target spot pathogen such as apple and pears.The anti-Target spot pathogen preparation of compound tea seed cake can have
Effect ground suppression kills the fruit trees ring spot mycelia such as apple and pears growth and spore germination and control its diffusion of stretching, inhibiting rate 80% with
On, as depicted in figs. 1 and 2.In addition said preparation also has effect preventive and therapeutic effect to scab of apple, peach brown rot etc..
2, germ is difficult to develop drug resistance.The anti-Target spot pathogen preparation of compound tea seed cake is a kind of pure natural, plant source preparation, together
When its complicated components, Target spot pathogen is difficult to develop drug resistance to it.
3, environmental protection, low toxicity.The primary chemical active constituent of the anti-Target spot pathogen preparation of compound tea seed cake is in growing process
The secondary metabolites of generation is easy to degrade in the environment.
4, raw materials for production are extensive, cheap, sustainable development.Convenient for comprehensive utilization of resources, it is suitble to large-scale production.This system
Plant used in agent is tea seed cake, tealeaves, herba fibraureae recisae root or stem, Schima superba blade, Leaf of Magnolia officinalis, rule grass, ginkgo leaf, and these types of plant is at me
State-owned relatively broad distribution.In addition, tea seed cake and tealeaves are the forestry by-product and leftover bits and pieces after oil plant or tea utilization.
5, acquired tea seed cake, tealeaves, herba fibraureae recisae root or stem, Schima superba blade, Leaf of Magnolia officinalis, rule grass, ginkgo leaf after being extracted using alcohol
Deng production waste residue can be used for producing organic fertilizer, it is not only environmentally friendly, but also be conducive to waste utilization.
6, the solvent in the technical process, eluant, eluent are reusable, and process costs are low, easy to industrialized production.
Detailed description of the invention
Fig. 1 is the inhibiting effect figure that grows to black rot of pear bacterium of various concentration Extracted From Oil-tea-cake extract solution, a, b, c in figure,
D, e, f, g respectively indicate Extracted From Oil-tea-cake extract concentrations be 0mg/mL, 5mg/mL, 6.6mg/mL, 10mg/mL, 13.3mg/mL,
15mg/mL, 20mg/mL.
Fig. 2 is tea seed cake extract to black rot of pear bacterium mycelia processing result figure, and wherein CK, a, b, c, d respectively indicate oil tea
Dregs of rice extract concentrations are 0mg/mL, 5mg/mL, 10mg/mL, 15mg/mL, 20mg/mL.
Specific embodiment
The present invention is further explained in the light of specific embodiments, following embodiment be intended to illustrate invention rather than
Limitation of the invention further.
Embodiment 1
The vegetable materials such as tea seed cake, tealeaves, herba fibraureae recisae root or stem, Schima superba blade, Leaf of Magnolia officinalis, rule grass, ginkgo leaf are placed in baking oven 70
It DEG C dries to constant weight, then weighs tea seed cake 50-100g, 20-30g parts of tealeaves, Schima superba blade 20-30g, Cortex Magnoliae Officinalis fresh leaf 7-10g, rule grass
It being crushed after 20-30g, ginkgo leaf 7-10g mixing, crosses 40 meshes, degreasing adds 70% alcohol steep of 1500-2000mL 3 times,
Extraction 8-12 hours every time, respectively through alcohol reflux extraction, filtering, reduced pressure, the operations such as freeze-drying obtain slightly promoting powder, as
Compound tea seed cake biological pesticide main effect component.
Herba fibraureae recisae root or stem are ground into coarse powder, and 0.2% sulfuric acid solution is added to extract 3 times, every time extraction 8-12 hours, filter, and receive
Collect filtrate, filtrate adds 7% to saltout to obtain thick promotion powder containing salt compounded of iodine.
Compound tea seed cake biological pesticide main effect component is promoted powder with herba fibraureae recisae root or stem thickness to mix by 10: 1-5: 1,40~50
Stirred at low speed at DEG C, be mixed to prepare finished product, in it is closed, be protected from light under the conditions of save or transport.
The ratio that drug is watered is 1: 200-500.
Embodiment 2
The resulting compound tea seed cake biological pesticide main effect component of Example 1, by extract mass gradient be diluted to 5mg/mL,
6.6 mg/mL, 10mg/mL, 13.3mg/mL, 15mg/mL, 20mg/mL, with mycelial growth rate method measurement extract to fruit tree
The inhibiting rate of Target spot pathogen mycelial growth.Each 1mL of preparation of 6 concentration gradients, control group are separately added into sterilizes culture dish
Add equivalent sterile water, pours into the PSA culture medium of preference temperature (the pushing time contacts non-scald on hand) in each culture dish respectively
9mL gently shakes up immediately, to be solidified.It is beaten with punch in 5 big eugonic fruit tree Target spot pathogen colony edges of culture and takes bacterium
Disk (diameter 9mm), one piece of each picking moves into corresponding plate, and the one side for having mycelia overturning is tipped upside down on culture base table downward
Face, tweezers press slightly to be made to be adjacent to, and is placed in 28 DEG C of insulating boxs and is cultivated 5d, 3 repetitions of every group of processing.Each processing group is observed daily
Colony diameter, and measured with cross phase multiplication and record and photograph, until stopping observation when control group almost covers with culture dish.
As a result as shown in Figure 1, compound tea seed cake preparation has clearly the growth of Target spot pathogen mycelia as can be seen from Figure 1
Inhibiting effect the inhibitory effect of Target spot pathogen is also enhanced, 0~10mg/mL's is antibacterial with the increase of preparation solution concentration
Effect increasing degree is maximum, and when concentration is in 6.6mg/mL, inhibiting rate reaches 70%, when more than 13.3mg/mL, antibacterial effect
Fruit increasing degree becomes smaller, and inhibiting rate is about 83%, and in 20mg/mL processing group, bacteriostasis rate reaches maximum.With cultivated days
Extend, in low concentration compound tea seed cake preparation solution processing group, Target spot pathogen is still in very slowly growth, to Target spot pathogen
Fungistatic effect have a little decrease, inhibiting rate is on a declining curve；And line is taken turns in high concentration compound tea seed cake preparation solution processing group
Although germ is also growing, inhibitory effect is then to continue with reinforcement, and fungistatic effect is preferable, handles in the 5th day 20 mg/mL
In group, inhibiting rate reaches maximum, is 86.54%.Wherein, the concentration of 13.3mg/mL processing group is not high and fungistatic effect compares
Steadily, for several days fungistatic effects 80% or more, this shows that the compound tea seed cake preparation of 13.3mg/mL concentration has Target spot pathogen
Good inhibitory effect.
Embodiment 3
The resulting compound tea seed cake biological pesticide main effect component of Example 1, by extract mass gradient be diluted to 20,15,10,
5 and 0mg/mL totally 5 concentration gradients mix.It is punched on cultured fungus culture vessel with punch, in each test tube
A piece of bacterium disk is respectively added, is cultivated three days in 28 DEG C.Mycelia smear is taken, sarranine redyes liquid dyeing immediately after being allowed to dry, under microscope
Observation.3 repetitions of every group of processing.The mycelium growthform (Fig. 2) of microexamination processing.
Fig. 2 is shown, in control group (CK), mycelia grows intact, Non Apparent Abnormality situation；In processing group, with oil tea
Phenomena such as raising of dregs of rice extract solution concentration, mycelia gradually appear swelling, fracture；Especially in 20mg/mL processing group, mycelia
Fragment into segment not of uniform size.Thus, it is indicated, that the elongation that compound tea seed cake biological pesticide can not only inhibit mycelia to grow
And sprouting, it may also lead to cellular lysate, plasm caused to leak.
Claims (7)
1. it is a kind of prevent and treat fruit tree ring spot biological pesticide, which is characterized in that the biological pesticide the preparation method is as follows:
The vegetable materials such as tea seed cake, tealeaves, herba fibraureae recisae root or stem, Schima superba blade, Leaf of Magnolia officinalis, rule grass, ginkgo leaf are placed in 70 DEG C of baking oven bakings
To constant weight, tea seed cake 50-100 parts by weight, tealeaves 20-30 parts by weight, Schima superba blade 20-30 parts by weight, Cortex Magnoliae Officinalis fresh leaf are then weighed
It is crushed after 7-10 parts by weight, rule grass 20-30 parts by weight, the mixing of ginkgo leaf 7-10 parts by weight, crosses 40 meshes, degreasing adds
70% alcohol steep 3 times, extraction 8-12 hours, extract through alcohol reflux respectively, filtering, are concentrated under reduced pressure every time, the operation such as freeze-drying
It obtains slightly promoting powder, as compound tea seed cake biological pesticide main effect component.
Herba fibraureae recisae root or stem are ground into coarse powder, and 0.2% sulfuric acid solution is added to extract 3 times, every time extraction 8-12 hours, and filter is collected in filtering
Liquid, filtrate add 7% to saltout to obtain thick promotion powder containing salt compounded of iodine.
Compound tea seed cake biological pesticide main effect component is promoted powder with herba fibraureae recisae root or stem thickness to mix by 10: 1-5: 1, at 40~50 DEG C
Stir at low speed, be mixed to prepare finished product, in it is closed, be protected from light under the conditions of save or transport.
The characteristics of compound tea seed cake biological pesticide preparation be with tea seed cake, tealeaves, herba fibraureae recisae root or stem, Schima superba blade, Leaf of Magnolia officinalis, rule grass,
Ginkgo biloba p.e is main sterilization component, and wettable powder is made.
2. a kind of application method for the compound tea seed cake biological pesticide for preventing and treating fruit tree ring spot, it is characterised in that: the prevention and treatment fruit tree
The biological pesticide of ring spot is used for fruit tree bud phase, growth period, period and Wintering Period, the drug of the prevention and treatment fruit tree ring spot
Method of administration is to be watered spraying or brush trunk, and the ratio that compound tea seed cake biological pesticide preparation is watered is 1: 200-500.Brush tree
Dry dose is that compound tea seed cake biological pesticide 100g is watered 10-30kg, brushes trunk.The dosage that blade face and branch spray is each
The biological pesticide 5-10kg after being watered dilution is sprayed per acre.It is primary every 15 days investigation fruit tree gardens, such as find fruit tree ring spot,
After removing diseased region, it will be sprayed on fruit tree branches and leaves or brush in trunks of fruit trees after the dilution of above-mentioned compound tea seed cake biological pesticide.
3. the preparation method of the biological pesticide of prevention and treatment fruit tree ring spot according to claim 1, it is characterised in that: the tea
The vegetable materials such as withered, tealeaves, herba fibraureae recisae root or stem, Schima superba blade, Cortex Magnoliae Officinalis fresh leaf, rule grass, ginkgo leaf should be current year new production, without mould
Become.It is placed in 70 DEG C of baking oven to dry to constant weight, then weighs tea seed cake, tealeaves, Schima superba blade, Cortex Magnoliae Officinalis fresh leaf, restrains weight described in grass, ginkgo leaf
Part is measured, is crushed after mixing, 40 meshes are crossed, degreasing adds 70% alcohol steep 3 times, every time extraction 8-12 hours, respectively through second
Alcohol reflux extraction, is concentrated under reduced pressure filtering, and the operations such as freeze-drying obtain slightly promoting powder, as compound tea seed cake biological pesticide main effect component.
Herba fibraureae recisae root or stem are ground into coarse powder, and 0.2% sulfuric acid solution is added to extract 3 times, every time extraction 8-12 hours, and filter is collected in filtering
Liquid, filtrate add 7% to saltout to obtain thick promotion powder containing salt compounded of iodine.
Compound tea seed cake biological pesticide main effect component is promoted powder with herba fibraureae recisae root or stem thickness and is mixed by 10: 1-5: 1, low at 40~50 DEG C
Speed stirring, be mixed to prepare finished product, in it is closed, be protected from light under the conditions of save or transport.
4. the preparation method of the biological pesticide of prevention and treatment fruit tree ring spot according to claim 1, it is characterised in that: described multiple
Square tea seed cake biological pesticide main effect component, need to it is closed, be protected from light under the conditions of save or transport.
5. the application method of the biological pesticide of prevention and treatment fruit tree ring spot according to claim 2, it is characterised in that: described multiple
Square tea seed cake biological pesticide method of administration is the dosage being administered after diluting are as follows: the compound tea seed cake biology agriculture of the prevention and treatment fruit tree ring spot
Medicine is 1: 200-500 for the ratio that when being watered spraying, compound tea seed cake biological pesticide preparation is watered, the use that blade face and branch spray
Amount is each biological pesticide 5-10kg sprayed after being watered dilution per acre.
6. the application method of the biological pesticide of prevention and treatment fruit tree ring spot according to claim 2, it is characterised in that: described anti-
Controlling dose of the compound tea seed cake biological pesticide of fruit tree ring spot for when brushing trunk, brushing trunk is compound tea seed cake biological pesticide
100g is watered 10-30kg, brushes trunk.
7. the application method of the biological pesticide of prevention and treatment fruit tree ring spot according to claim 2, it is characterised in that: described anti-
The compound tea seed cake biological pesticide of fruit tree ring spot is controlled in fruit tree bud phase, growth period, period and Wintering Period, every 15-30 days
It is primary to investigate fruit tree garden, such as finds fruit tree ring spot, after removing diseased region, after the dilution of above-mentioned compound tea seed cake biological pesticide
It is sprayed on fruit tree branches and leaves or brushes in trunks of fruit trees.
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Cited By (1)
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| CN114938808A (en) * | 2022-06-24 | 2022-08-26 | 贵州大学 | Magnolia officinalis alkali and nuciferine medicinal composition and preparation method thereof |
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| CN109042759A (en) * | 2018-09-11 | 2018-12-21 | 苏州科技大学 | A kind of biological pesticide preparation of compound tea seed cake blast resisting |
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| CN114938808B (en) * | 2022-06-24 | 2023-05-26 | 贵州大学 | Magnolol and nuciferine pharmaceutical composition and preparation method thereof |
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