CN101422162A - Plant source preparation for preventing and treating Meloidogyne incognita disease and preparation method thereof - Google Patents
Plant source preparation for preventing and treating Meloidogyne incognita disease and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a plant source preparation for controlling root-knot nematode and a preparation method thereof, which belongs to the technical field of plant source pesticide. According to the weight percentage, the preparation thereof consists of 30 to 80 percent of plant carbon, 10 to 50 percent of Chinese medicinal herb diluent as well as 1 to 20 percent of degreasing and degumming bone powder. The preparation method thereof is that the plant carbon is cracked and passes a screen of 2mm; 10 to 40 percent of marigold, 10 to 40 percent of barberry, 10 to 40 percent of sheep hoof, 10 to 30 percent of groundsel and 10 to 40 percent of china root are cracked and mixed into a mixed Chinese medicinal herb; the mixed Chinese medicinal herb is dipped by table vinegar liquid for 150 to 160 hours; and then the filter liquid is distilled for 10 to 20 times, thus obtaining the Chinese medicinal herb diluent. After the plant carbon and the degreasing and degumming bone powder are fully mixed, and then the Chinese medicinal herb diluent is added and fully stirred. The relative control effect to the root-knot nematode is remarkable and achieves 86 to 100 percent; the crop yield is improved by 19 to 82 percent; and the transplanting surviving rate of seedlings is improved by 53 percent.
Description
Technical field
The present invention relates to the botanical pesticide technical field, be specifically related to a kind of root-knot nematode diseases such as silkworm and mulberry, vegetables, banana, konjaku, flue-cured tobacco, lawn, green plants that are used to effect a radical cure, can promote beneficial microorganism growth and breeding in the soil again, and have plant resource new formulation of certain fertilizer efficiency and preparation method thereof.
Background technology
For a long time, the control of plant root-knot nematode is a global difficult problem always.It also is the external quarantine object of countries in the world.Root-knot nematode to plant, the harm of crops has been reached the lighter's underproduction, the extents of injury that weight person is all dead.The crop root-knot nematode also can be caused more serious harm to crop with the compound crop root that infects of other pathogenic pathogen.After crops were subjected to root-knot nematode harm, plant showed dwarfing, yellow and withered gradually, the plant husbandry industry that has, and the harm that is subjected to root-knot nematode owing to crop is uncontrollable, even has reached the degree of bankruptcy.At present, the generation area of root-knot nematode enlarges year by year, the crop of serious harm has important economic crops such as flue-cured tobacco, silkworm and mulberry, banana, fruit tree, vegetables, flowers, medicinal material, and the control of root-knot nematode has become crops and produced a problem and a difficult problem that presses for solution.Root-knot nematode has host crop kind more than 3000, and generally between 10%-40%, high reaches 70% to the extent of injury.Crop in Yunnan Province's serious harm has important economic crops such as flue-cured tobacco, silkworm and mulberry, banana, fruit tree, vegetables, flowers, medicinal material, area takes place reach more than 1,500 ten thousand mu.
The harm of control root-knot nematode in the prior art, main iron go out gram, the contour poison of furans pellet, the high residue chemical pesticide of using, the defective of its existence is the polluted underground water source, produce residue of pesticide, cause environmental pollution, not only to environment, health is brought certain harm, because agricultural chemicals is difficult to directly arrive in the body of soil Rigen tie lines worm, often do not reach the effect of thorough control simultaneously.
In recent years, also develop microbial bacterial agent (mixture that comprises microorganism and botanical material) and plant in the prior art and mix mematocide (mixture of botanical material) control root-knot nematode, but also have problem and defective such as the relatively poor or control efficiency instability of control efficiency.
Therefore, seeking a kind ofly can have favorable effect to root-knot nematode, the control medicine that environment is not polluted again, non-chemically prevent and treat the research and development of medicine, it is plant protection scientific and technical personnel's research direction, also be the research direction of control root-knot nematode, develop such plant resource control root-knot nematode preparation and also have most application prospect and value.
Summary of the invention
The present invention seeks to overcome high poison, high residue contaminated environment, polluted underground water source that chemical pesticide control root-knot nematode in the prior art exists, be detrimental to health and be difficult to directly arrive in the soil Rigen tie lines polypide, do not reach the defective of thorough control efficiency, and the control efficiency difference and the unsettled defective of preventive effect of microbial bacterial agent and other control root-knot nematode existence, provide a kind of have lasting control efficiency, pollution-free, control efficiency is remarkable, can promote novel plant source preparation of plant growing and preparation method thereof again.
The plant resource pulvis of gained of the present invention, it is preparation through the control root-knot nematode of developing after inventor's experiment (test), demonstration and experience accumulation for many years, can be used for effecting a radical cure root-knot nematode diseases such as silkworm and mulberry, vegetables, banana, flue-cured tobacco, lawn, green plants, can promote beneficial microorganism growth and breeding in the soil again.
The principle that preparation of the present invention is killed root-knot nematode is: utilize the plant charcoal that is applied in the soil, constantly discharge the medium-height grass the effective elements of the medicine.In several Chinese herbal medicines of being prepared, each Chinese herbal medicine all has anti-inflammatory, the effect of the pain that disappears and desinsection, Aztec marigold particularly, it contains special metabolite α-terthienyl, it can be in elegans cell oxidation cell membrane lipid, protein, amino acid, sterols, its function is affected, cause cell death, finally make nematode death, this effective ingredient material all has remarkable preventive effect to various plants insect and nematode.In addition, the insecticidal action of other several Chinese herbal medicines is also slowly discharged by plant charcoal, causes nematode constantly to suck traditional Chinese medicine ingredients in large quantities, and the material such as protein, amino acid of elegans cell is damaged, and causes nematode death at last.When killing nematode, suppress the breeding of pathogen in the soil, the protective plant root is not subjected to or lacks the invasion and attack of nematode or pathogen, makes peripheral root-knot nematode be difficult to invade the dispenser position, thereby makes the growth of crop root health.
The present invention compared with prior art has following beneficial effect:
1, all raw materials of this preparation are the plant resource preparations all from plant, and the advantage that compares with microbial bacterial agent control root-knot nematode is that control efficiency is stable.
2, all raw materials of this preparation are the plant resource preparations all from plant, can not pollute environment, also can not produce residually, can not work the mischief to environment and people and animals.
3, according to the control test of product of the present invention to the root-knot nematode of Different Crop, this preparation has reached 86%-100% to the relative control efficiency of root-knot nematode, and the control efficiency highly significant used the soil of this preparation, the root-knot nematode storage seldom, the plant growth stalwartness.
4, the reasonable auxiliary material of the reasonably combined and preparation of various Chinese herbal medicine sterilizations, antiseptic property in this preparation, make and be applied in behind the crop root also having good sterilization, disinfective action around the crop root, can increase beneficial microorganism breeding and growth in the soil environment, can effectively decompose nitrogen sulphur ammoniate in the soil, poisonous metal element in the absorption soil, reduce the residual of agricultural chemicals, chemical fertilizer, strengthen the permeability of soil, promote and the adjusting plant growth.
5, after this preparation uses, when killing Plant nematode, have good fertilizer efficiency, used the crop of this preparation, need not use other chemical fertilizer again, also can satisfy the fertilizer needs of crop substantially.It can promote the well developed root system of plant, can effectively increase survival rate to crop, nursery stock transplanting, and the efficacy of a drug, the fertility term of validity had the characteristics of water conservation, slowly-releasing for eight months.Its mulberry leaf output has increased production 19% approximately, and tomato yield has increased production 69% approximately, and yield of flue-cured tobacco has increased production 82% approximately, and the transplanting survival rate of nursery stock improves 53%.
6, the raw material abundance of this preparation, it is more to originate.
Concrete technical scheme of the present invention is as follows:
One, the plant resource preparation of control root-knot nematode of the present invention, form by following component and percentage by weight:
Plant charcoal 30%-80%, Chinese herbal medicine dilution 10%-50%, degreasing degelatinized bone meal 1%-20%; Described Chinese herbal medicine dilution is by vinegar liquid: mixing Chinese herbal medicine is 3: part by weight, soak with vinegar liquid and to mix Chinese herbal medicine after 150-160 hour, remove by filter the dregs of a decoction, get its filtrate dilution 10-20 and doubly get; Described mixing Chinese herbal medicine is that the Chinese herbal medicine of Aztec marigold 10%-40%, barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40% is formed by percentage by weight and component, and its Chinese herbal medicine is cut into pure piece or powder are pure to become the composite mixing Chinese herbal medicine of chaff shape.
Above-mentioned 5 flavor herbal mediciments can be buied from pharmacy.The degreasing degelatinized bone meal also is commercially available.
Two, the preparation method of the plant resource preparation of control root-knot nematode of the present invention, carry out according to the following steps:
1, by weight percentage, get plant charcoal 30%-80%, and with pure 2 mm sieve of crossing of plant powdered carbon.
The concrete preparation method of described plant charcoal according to Chinese patent, its number of patent application of this part inventor application is: 200610163868.9, denomination of invention is carried out for the technical scheme of " a kind of plant refuse comprehensive utilization method ", and the crop straw carbide that is made into by this technical scheme is plant charcoal of the present invention.
2, preparation Chinese herbal medicine dilution, 1. be that the Chinese herbal medicine of Aztec marigold 10%-40%, barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40% is cut into respectively that pure piece or powder are pure to become the chaff shape with component by weight percentage, and be mixed with into the mixing Chinese herbal medicine; 2. press vinegar liquid: mix the part by weight of Chinese herbal medicine=3:1, the mixing Chinese herbal medicine with 1. vinegar liquid soaking step prepares soaked 150-160 hour, removed by filter the dregs of a decoction, got its filtrate dilution 10-20 and doubly was described Chinese herbal medicine dilution;
3, by weight percentage, extracting degreasing degelatinized bone meal 1%-20% is mixed into compound with above-mentioned plant charcoal 30%-80% and degreasing degelatinized bone meal 1%-20%;
4, be the Chinese herbal medicine dilution of step 2 preparation that 10%-50% ratio adds in the compound of step 3 by weight percentage again, stir, pack, promptly get the plant resource preparation of preventing and treating root-knot nematode of the present invention.
Above-mentioned 5 flavor Chinese herbal medicines can be buied from pharmacy, and the degreasing degelatinized bone meal also is commercially available.
The using method of product of the present invention:
When 1, being used for woody plant, plant root annular trench digging, ditch depth with can see the stem root, fibrous root is as the criterion, furrow width is generally 15-30cm according to tree vigo(u)r (size of tree, tree crown situation).Product of the present invention is evenly spread fertilizer over the fields in annular ditch, and earthing gets final product.
2, be used for flowers crops such as Chinese rose, lily, carnation, can after crop two is surveyed the trench digging row replacement, get final product by earthing.
3, be used for flue-cured tobacco, tomato, Chinese cabbage etc. crops, carry out the pool and execute when transplanting, every strain 30-50 gram can effectively be prevented and treated root-knot nematode, and promotes the crop healthy growth.
Embodiment
Below in conjunction with specific embodiment, can further be well understood to the present invention, but it is not the qualification to protection domain of the present invention.
Embodiment 1
One, the plant resource preparation of control root-knot nematode of the present invention, form by following component and percentage by weight:
Plant charcoal 70%, bone meal 5%, Chinese herbal medicine dilution 25%; Described Chinese herbal medicine dilution is by vinegar liquid: mix the part by weight of Chinese herbal medicine=3:1, soak with vinegar liquid and mix Chinese herbal medicine after 150 hours, remove by filter the dregs of a decoction, get its 10 times of filtrate dilution and get; Described mixing Chinese herbal medicine by percentage by weight and component be that Aztec marigold accounts for 10%, the Chinese herbal medicine of barberry 40%, sheep hoof 25%, climbing groundsel 10%, smilax 15% forms, and its Chinese herbal medicine is cut into pure piece or powder are pure to become the composite mixing Chinese herbal medicine of chaff shape.
Two, the preparation method of above-mentioned plant resource preparation, carry out according to the following steps:
1, the percentage by weight of getting plant charcoal is 70%, and with pure 2 mm sieve of crossing of plant powdered carbon.
The concrete preparation method of described plant charcoal according to Chinese patent, its number of patent application of this part inventor application is: 200610163868.9, denomination of invention is carried out for the technical scheme of " a kind of plant refuse comprehensive utilization method ", and the crop straw carbide that is made into by this technical scheme is plant charcoal of the present invention.
2, used Chinese herbal medicine dilution among preparation the present invention, 1. be with component that Aztec marigold accounts for 10% by weight percentage, the Chinese herbal medicine of barberry 40%, sheep hoof 25%, climbing groundsel 10%, smilax 15% is cut into pure piece respectively or powder is pure becomes the chaff shape, and is mixed with into the mixing Chinese herbal medicine; 2. press vinegar liquid: mix the part by weight of Chinese herbal medicine=3:1, the mixing Chinese herbal medicine with 1. vinegar liquid soaking step prepares soaked 150 hours, removed by filter the dregs of a decoction, got its filtrate dilution and was Chinese herbal medicine dilution used in the invention described above for 10 times.
3, by weight percentage, extracting degreasing degelatinized bone meal 5% is mixed into compound with above-mentioned plant charcoal 70% and degreasing degelatinized bone meal 5%;
4, be the Chinese herbal medicine dilution of step 2 preparation that 25% ratio adds in the compound of step 3 by weight percentage again, stir, pack, promptly get the plant resource preparation that the invention described above is prevented and treated root-knot nematode.
Above-mentioned 5 flavor Chinese herbal medicines can be buied from pharmacy, and the degreasing degelatinized bone meal also is commercially available.
Three, the application of above-mentioned plant resource preparation
Plant resource formulation products with above-mentioned preparation, entrust silkworm and mulberry station, Luliang County, Yunnan Province that the silkworm and mulberry root-knot nematode is prevented and treated test, mulberry tree field piece (mulberry tree breed is Mi Sang) at the age that root-knot nematode is taken place, arrange to handle with two processing of contrast and carry out with the field comparative trial, that handles uses preparation 50 gram/strains of the present invention on every side at the mulberry tree root, do not execute other fertilizer, any medicament is not used in contrast, conventional fertilizer application, after a season of growth (8 months), carry out the control efficiency investigation of root nematode, the result, the root nematode diseased plant rate of using preparation processing of the present invention is 14.3%, control treatment root nematode diseased plant rate is 100%, and the nematode occurrence degree is serious, with diseased plant rate, use preparation of the present invention the relative control effect rate of mulberry tree root-knot nematode is reached 85.7%, investigate the output of mulberry leaf, the sub-district area is 21 square metres, the mulberry leaf output of handling the sub-district is 53.6 kilograms, and the mulberry leaf output of control plot is 45.2 kilograms, handles the mulberry leaf output of sub-district silkworm and mulberry and has increased production 18.58%, the efficacy of a drug, fertility has continued about 8 months, utilization benefit fairly obvious (embodiment 1-4 also can be referring to table 1 to the root-knot nematode control efficiency).
Embodiment 2
One, the plant resource preparation of control root-knot nematode of the present invention, form by following component and percentage by weight:
Plant charcoal 80%, bone meal 10%, Chinese herbal medicine dilution 10%; Described Chinese herbal medicine dilution is by vinegar liquid: mix the part by weight of Chinese herbal medicine=3:1, soak with vinegar liquid and mix Chinese herbal medicine after 160 hours, remove by filter the dregs of a decoction, get its 20 times of filtrate dilution and get; Described mixing Chinese herbal medicine is that the Chinese herbal medicine of Aztec marigold 40%, barberry 10%, sheep hoof 10%, climbing groundsel 30%, smilax 10% is formed by percentage by weight and component, and its Chinese herbal medicine is cut into pure piece or powder are pure to become the composite mixing Chinese herbal medicine of chaff shape.
Two, the preparation method of above-mentioned plant resource preparation, remove component and percentage by weight in the used plant resource preparation, vinegar liquid soak time, mix component and percentage by weight in the Chinese herbal medicine and press the component of the foregoing description 2 and weight percent when outside the vinegar liquid soak time, all the other preparation methods are identical with the preparation method of embodiment 1 described plant resource preparation, repeat no more.
Three, the application of above-mentioned plant resource preparation
Plant resource formulation products with above-mentioned preparation, entrust Yunnan Province Yuanmou County means of agricultural production specialty cooperative society that tomato root-knot eelworm is prevented and treated test, at the tomato field piece (tomato variety is tomato No. 4) that root-knot nematode is taken place, arrange to handle with two processing of contrast and carry out with the field comparative trial, preparation 30 gram/strains of the present invention are used in transplanting in the pool tomato of handling, any medicament is not used in contrast, after a season of growth, carry out the control efficiency investigation of root nematode, the result, the average diseased plant rate of root nematode of using the processing of preparation of the present invention is 5.33%, and disease index is 1.3, and the root nematode diseased plant rate of control treatment is 100%, and occurrence degree is serious, disease index is 27.65, uses preparation of the present invention the relative control effect of tomato root-knot eelworm is reached 95.3%, and the sub-district area is 30 square metres, the tomato yield of handling the sub-district is 269.86 kilograms, the tomato yield of control plot is 159.87 kilograms, and the tomato yield of handling the sub-district has increased production 68.8%, and utilization benefit is very remarkable.
Embodiment 3
One, the plant resource preparation of control root-knot nematode of the present invention, form by following component and percentage by weight:
Plant charcoal 30%, bone meal 20%, Chinese herbal medicine dilution 50%; Described Chinese herbal medicine dilution is by vinegar liquid: mix the part by weight of Chinese herbal medicine=3:1, soak with vinegar liquid and mix Chinese herbal medicine after 155 hours, remove by filter the dregs of a decoction, get its 15 times of filtrate dilution and get; Described mixing Chinese herbal medicine is that the Chinese herbal medicine of Aztec marigold 10%, barberry 10%, sheep hoof 40%, climbing groundsel 30%, smilax 10% is formed by percentage by weight and component, and its Chinese herbal medicine is cut into pure piece or powder are pure to become the composite mixing Chinese herbal medicine of chaff shape.
Two, the preparation method of above-mentioned plant resource preparation, remove component and percentage by weight in the used plant resource preparation, vinegar liquid soak time, mix component and percentage by weight in the Chinese herbal medicine and press the component of the foregoing description 3 and weight percent when outside the vinegar liquid soak time, all the other preparation methods are identical with the preparation method of embodiment 1 described plant resource preparation, repeat no more.
Three, the application of above-mentioned plant resource preparation
Utilize the plant resource formulation products of above-mentioned preparation, entrust Yunnan Provine Tobacco Science Inst. that the flue-cured tobacco root-knot nematode is prevented and treated test, plant vega piece (tobacco bred is a cloud and mist 87) what root-knot nematode throughout the year took place, arrange to handle with two processing of contrast and carry out with the field comparative trial, that handles uses preparation 30 gram/strains of the present invention in the flue-cured tobacco transplanting pool, any medicament is not used in contrast, after a season of growth, carry out the control efficiency investigation of root nematode, the result, use the diseased plant rate 21.1% of the root root-knot nematode of preparation processing of the present invention, disease index is 2.72, and the root nematode diseased plant rate of control treatment is 83.3%, and occurrence degree is serious, disease index is 46.89, uses preparation of the present invention the relative control effect of flue-cured tobacco root-knot nematode is reached 94.2%.The sub-district area is 36 square metres, and the flue-cured tobacco per mu yield of handling the sub-district is 173.8 kilograms, and the flue-cured tobacco per mu yield of control plot is 95.39 kilograms, handles the flue-cured tobacco per mu yield of sub-district and has increased production 82.2%, and utilization benefit is very remarkable.
Embodiment 4
One, the plant resource preparation of control root-knot nematode of the present invention, form by following component and percentage by weight:
Plant charcoal 80%, bone meal 1%, Chinese herbal medicine dilution 19%; Described Chinese herbal medicine dilution is by vinegar liquid: mix the part by weight of Chinese herbal medicine=3:1, soak with vinegar liquid and mix Chinese herbal medicine after 158 hours, remove by filter the dregs of a decoction, get its 18 times of filtrate dilution and get; Described mixing Chinese herbal medicine is that the Chinese herbal medicine of Aztec marigold 10%, barberry 20%, sheep hoof 20%, climbing groundsel 10%, smilax 40% is formed by percentage by weight and component, and its Chinese herbal medicine is cut into pure piece or powder are pure to become the composite mixing Chinese herbal medicine of chaff shape.
Two, the preparation method of above-mentioned plant resource preparation, remove component and percentage by weight in the used plant resource preparation, vinegar liquid soak time, mix component and percentage by weight in the Chinese herbal medicine and press the component of the foregoing description 4 and weight percent when outside the vinegar liquid soak time, all the other preparation methods are identical with the preparation method of embodiment 1 described plant resource preparation, repeat no more.
Three, the application of above-mentioned plant resource preparation
Plant resource formulation products with above-mentioned preparation, entrust Wuhua District afforestation place of Kunming, Yunnan Province that ornamental trees and shrubs are carried out root-knot nematode control test, (the nursery stock kind is Yunnan Piao to 10000 strains of plantation greening seedling on the barren hill vacant land, plant height 40-50 centimetre), arrange to handle with two processing of contrast and compare test, wherein handle 9900 strains, contrast 100 strains, that handles uses preparation 50 gram/strains of the present invention in the transplanted plantlet pool, any medicament is not used in contrast, compare through transplanting latter two month, the nursery stock of handling has survived 9405 strains, transplanting survival rate has reached 95%, and growth potential is good, the glossy sense of leaf look dark green, 100 young plant wood of contrast have only survived 42 strains, transplanting survival rate only is 42%, and a little less than the growth potential, the control efficiency selective examination (100 strain) of root nematode is carried out in the tarnish of leaf look pale yellow, and the root nematode diseased plant rate of using preparation processing of the present invention is 0, the root nematode incidence of control treatment is 16%, use preparation of the present invention the relative control effect of nursery stock root-knot nematode is reached 100%, the transplanting survival rate of nursery stock has improved 53%, and application benefit is very remarkable.
Processing that table 1 embodiment 1-4 uses preparation of the present invention and root-knot nematode control efficiency with the check plot, field
Root nematode diseased plant rate | Disease index | Relative control effect rate to root-knot nematode | The cell production (kilogram) that the line enemy handles | Crop yield | |
Embodiment 1 uses the processing (silkworm and mulberry) of preparation of the present invention | 14.3% | 1.12 | 85.7% | 53.6 | 18.58% |
The same field contrast of embodiment 1 | 100% | 7.83 | - | 45.2 | - |
Embodiment 2 uses the processing (tomato) of preparation of the present invention | 5.33% | 1.3 | 95.3% | 269.86 | 68.8% |
The same field contrast of embodiment 2 | 100% | 27.65 | - | 159.87 | - |
Embodiment 3 uses the processing (flue-cured tobacco) of preparation of the present invention | 21.1% | 2.72 | 94.2% | 173.8 | 82.2% |
The same field contrast of embodiment 3 | 83.3% | 46.89 | - | 95.39 | - |
Embodiment 4 uses the processing (sapling) of preparation of the present invention | 0 | 0 | 100% | The nursery stock transplanting survival rate reaches 95% | The nursery stock transplanting survival rate has improved 53% |
The same field contrast of embodiment 4 | 16% | 1.54 | The nursery stock transplanting survival rate is 42% |
Above data computing method or formula are as follows:
Claims (2)
1, a kind of plant resource preparation of preventing and treating root-knot nematode is characterized in that, forms by following component and percentage by weight:
Plant charcoal 30%-80%, Chinese herbal medicine dilution 10%-50%, degreasing degelatinized bone meal 1%-20%; Described Chinese herbal medicine dilution is by vinegar liquid: mixing Chinese herbal medicine is the part by weight of 3:1, soaks with vinegar liquid and mixes Chinese herbal medicine after 150-160 hour, removes by filter the dregs of a decoction, gets its filtrate dilution 10-20 and doubly gets; Described mixing Chinese herbal medicine is that the Chinese herbal medicine of Aztec marigold 10%-40%, barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40% is formed by percentage by weight and component, and its Chinese herbal medicine is cut into pure piece or powder are pure to become the composite mixing Chinese herbal medicine of chaff shape.
2, the preparation method of the described plant resource preparation of claim 1 is characterized in that, carries out according to the following steps:
(1) by weight percentage, get plant charcoal 30%-80%, and with pure 2 mm sieve of crossing of plant powdered carbon;
(2) preparation Chinese herbal medicine dilution, 1. be that the Chinese herbal medicine of Aztec marigold 10%-40%, barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40% is cut into respectively that pure piece or powder are pure to become the chaff shape with component by weight percentage, and be mixed with into the mixing Chinese herbal medicine; 2. press vinegar liquid: mix the part by weight of Chinese herbal medicine=3:1, the mixing Chinese herbal medicine with 1. vinegar liquid soaking step prepares soaked 150-160 hour, removed by filter the dregs of a decoction, got its filtrate dilution 10-20 and doubly was described Chinese herbal medicine dilution;
(3) by weight percentage, extracting degreasing degelatinized bone meal 1%-20% is mixed into compound with above-mentioned plant charcoal 30%-80% and degreasing degelatinized bone meal 1%-20%;
(4) be the Chinese herbal medicine dilution of step (2) preparation that 10%-50% ratio adds in the compound of step (3) by weight percentage again, stir, pack, promptly get a kind of plant resource preparation of preventing and treating root-knot nematode of the present invention.
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