CN103314814B - A kind of application of plant resource preparation in control notoginseng root rot preventing and treating root-knot nematode - Google Patents

A kind of application of plant resource preparation in control notoginseng root rot preventing and treating root-knot nematode Download PDF

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CN103314814B
CN103314814B CN201310289581.0A CN201310289581A CN103314814B CN 103314814 B CN103314814 B CN 103314814B CN 201310289581 A CN201310289581 A CN 201310289581A CN 103314814 B CN103314814 B CN 103314814B
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herbal medicine
chinese herbal
root
knot nematode
plant
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CN103314814A (en
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何英杰
赵振玲
刘春美
彭秀玲
薛淑昆
陈忠华
杨昕
杨自琼
杨亮宇
李周兴
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KUNMING XIANDI BIOTECHNOLOGY Co Ltd
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KUNMING XIANDI BIOTECHNOLOGY Co Ltd
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Abstract

The present invention discloses a kind of application of plant resource preparation in control notoginseng root rot preventing and treating root-knot nematode, and the plant resource preparation of described control root-knot nematode has plant charcoal 30%-80%, Chinese herbal medicine dilution 10%-50%, degreasing degelatinized bone meal 1%-20%; Described Chinese herbal medicine has Aztec marigold 10%-40%, barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40%.Application control notoginseng root rot of the present invention, its diseased plant rate average out to 7.267% is that a large amount of economic loss has been retrieved in seven agricultures.The inventive method have pollution-free, without residual hazard, non-killed natural enemies, do not develop immunity to drugs, be conducive to the advantage such as person poultry safety and environmental protection, cost is low, anti-double controlling of holding concurrently, and is a kind of nuisanceless plant protection new method.

Description

A kind of application of plant resource preparation in control notoginseng root rot preventing and treating root-knot nematode
Technical field
The present invention relates to control of plant disease technology, be specifically related to apply a kind of plant resource preparation control notoginseng root rot preventing and treating root-knot nematode.
Background technology
Pseudo-ginseng (Panax notoginseng (Burk.) F.H.Chen.) is Araliaceae (Araliaceae) Panax (Panax) perennial plant, it is Chinese distinctive rare traditional Chinese medicine, also be the main component of Yunnan Baiyao, there are hemostasis, the effect such as promoting blood circulation and removing blood stasis, can be used for treating various haemorrhage and various cardio-cerebrovascular diseases.Pseudo-ginseng is typical cloudy raw C 3plant, needs to cultivate under the condition of sheltering from heat or light, and growing environment is shady, wet, can cause the occurrence injury of multiple diseases, particularly root rot, become the serious hindrance that notoginseng planting industry develops at present.Notoginseng root rot morbidity wide, all there is generation in all producing regions, and disease causes overground part plant Huang to wither, underground part stem tuber cracking, rot.The principal causative pathogen of notoginseng root rot is Pseudomonas alba (Pseudomonas sp.), fusarium solani (Fusarium solani), alternaria tenuis bacterium (Alternaria tenuis), Phoma herbarum (Phoma herbarum), Phytophthora cactorum (Phytophthora cactorum), Rhizoctonia solani Kuhn (Rhizoctonia solani), post spore mould (Cylindrocarpon sp.) etc., cause rotten pathogen and be mainly Fusariumsp, post spore is mould, Phytophthora cactorum, pratylenchus (Pratylenchus Filipev), pythium spp (Pythium Pringsheim), water mo(u)ld (Saprolegnia Nees) etc.
Notoginseng root rot study on prevention to be carried out for many years, in selection of land, crop rotation, anosis healthy and strong seedling application, cool canopy material and light transmittance are selected, seven gardens are cleaned, liquid manure reasonable management, prevention & protection (ditching draining, dispenser prevents), the aspects such as chemical control obtain multinomial achievement, are that corresponding economic loss has been retrieved in seven agricultures, but still there are problems in administering: 1, though effect good processing soil treatment agent Celfume is efficient, wide spectrum, soil penetration power is strong, residual few soil fumigant, to the bacterium in soil, fungi, nematode, killing of weeds is very competent, other soil disinfectant cannot be by comparison, but it is again a kind of material of serious ozone layer depletion, calendar year 2001 has been prohibited to produce and use, its substitute products are dazomet and Carbathiin, though particularly nematicide ability is strong in the raw effect of going out of these two kinds of materials, control sickle-like bacteria, Verticillium bacterium, pythium spp, the ability of the deep subsoil pathogens such as water mo(u)ld, in addition must pour water in use to help its liquid to spread in soil, be awkward like this, it is have more than is needed that hillside fields and arid there are not water area to make, and pseudo-ginseng is multiple plants at hillslope, so the application that pseudo-ginseng produces is little, 2, the bio-bacterial manure such as mixture, bacillus subtilis of chitin kind, microorganism and botanical material also has a certain amount of application on producing, but control efficiency is not obvious.3, conventional control is main still chemically at present.Conventional following various medicaments mixture uses: the bactericide such as 200ppm agricultural streptomycin, 25% Yekuling, 58% metalaxyl-mn-zn, 50% carbendazim, 64% Sandofan pulvis, carbendazim, metalaxyl, tmtd, zineb, Yekuling, bacillus subtilis.But the effect of these chemical agents or biologic product control is all poor, so, since pseudo-ginseng artificial planting, notoginseng root rot always is that pseudo-ginseng produces a upper main restricting factor, finds a kind of good method, good this disease of technology prevention become pseudo-ginseng and produced and go up problem in the urgent need to address and technical barrier.
Summary of the invention
The technical problem to be solved in the present invention is the technical barrier overcoming prior art control notoginseng root rot weak effect.
For solving the problems of the technologies described above, the invention provides a kind of application of plant resource preparation in control notoginseng root rot preventing and treating root-knot nematode, described a kind of plant resource preparation preventing and treating root-knot nematode forms by following component and percentage by weight: plant charcoal 30%-80%, Chinese herbal medicine dilution 10%-50%, degreasing degelatinized bone meal 1%-20%; Described Chinese herbal medicine dilution is by vinegar liquid: mixing Chinese herbal medicine is the part by weight of 3:1, after 150-160 hour, crosses and filters the dregs of a decoction, get its filtrate dilution 10-20 and doubly obtain with vinegar immersion bubble mixing Chinese herbal medicine; Described mixing Chinese herbal medicine by percentage by weight and component be Aztec marigold 10%-40%, the Chinese herbal medicine of barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40% forms, and its Chinese herbal medicine is cut into pure block or shatters into the mixing Chinese herbal medicine that chaff shape is mixed into.
The described a kind of application of plant resource preparation in control notoginseng root rot preventing and treating root-knot nematode, can carry out according to the following steps:
(1), when transplanting panax notoginseng bar, moisture in the soil face is spread fertilizer over the fields the plant resource preparation of described control root-knot nematode, and consumption is 80kg/ mu;
(2), after group bar is emerged, the plant resource imposing described control root-knot nematode in leaf stretching period and green sub-phase is respectively formed on plantation pool soil table once, and each consumption is 80kg/ mu;
(3) Second Year: impose the plant resource preparation of described control root-knot nematode respectively in pseudo-ginseng leaf stretching period and green sub-phase in plantation pool soil table once, each consumption is 80kg/ mu.
Described a kind of plant resource preparation preventing and treating root-knot nematode is that the applicant has applied for Chinese patent on December 9th, 2008 and authorized, its patent No. is: ZL200810233694.8, denomination of invention is: a kind of plant resource preparation preventing and treating root-knot nematode and preparation method thereof "; publication date: on 05 06th, 2009, publication number: CN101422162A.
Compared with prior art, the invention has the beneficial effects as follows:
1, instant invention overcomes the technical barrier of prior art control notoginseng root rot weak effect.Adopt the present invention, notoginseng root rot average onset strain rate is 7.267%, and the average onset strain rate of existing control notoginseng root rot is 41.8%, the inventive method is than the diseased plant rate decreased average 82.6% of existing control notoginseng root rot, decreased average loses 55253 yuan/mu, i.e. decreased average economic loss 82.6%, creates unforeseeable technique effect.
The plant resource preparation of the control root-knot nematode 2, described in the inventive method is Eco-friendly product; be applied to the control of notoginseng root rot; belong to ecological management plant pest category; have pollution-free, without residual hazard, non-killed natural enemies, do not develop immunity to drugs; be conducive to the advantage such as person poultry safety and environmental protection; cost is low, anti-double controlling of holding concurrently; and create unforeseeable technique effect; it is a kind of nuisanceless plant protection new method; significant economic benefit, social benefit and ecological benefits can be realized, meet the development of sustainable development and the ecological agriculture.
Embodiment
1, used a kind of of the present invention prevents and treats formula of the plant resource preparation of root-knot nematode and preparation method thereof
Used a kind of of the present invention prevents and treats formula of the plant resource preparation of root-knot nematode and preparation method thereof and has applied for Chinese patent by the applicant on December 9th, 2008 and authorized, its patent No. is: ZL200810233694.8, denomination of invention is: a kind of plant resource preparation preventing and treating root-knot nematode and preparation method thereof "; publication date: on 05 06th, 2009, publication number: CN101422162A.
Used a kind of of the present invention prevents and treats the plant resource preparation of root-knot nematode by following component and percentage by weight composition: plant charcoal 30%-80%, Chinese herbal medicine dilution 10%-50%, degreasing degelatinized bone meal 1%-20%; Described Chinese herbal medicine dilution is by vinegar liquid: mixing Chinese herbal medicine is the part by weight of 3:1, after 150-160 hour, crosses and filters the dregs of a decoction, get its filtrate dilution 10-20 and doubly obtain with vinegar immersion bubble mixing Chinese herbal medicine; Described mixing Chinese herbal medicine by percentage by weight and component be Aztec marigold 10%-40%, the Chinese herbal medicine of barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40% forms, and its Chinese herbal medicine is cut into pure block or shatters into the mixing Chinese herbal medicine that chaff shape is mixed into.
Described a kind of preparation method preventing and treating the plant resource preparation of root-knot nematode, carries out according to the following steps:
(1) by weight percentage, get plant charcoal 30%-80%, and plant charcoal was shattered 2 mm sieve;
(2) Chinese herbal medicine dilution is prepared, 1. be Aztec marigold 10%-40% with component by weight percentage, the Chinese herbal medicine of barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40% is cut into pure block respectively or shatters into chaff shape, and being mixed with into mixing Chinese herbal medicine; 2. press vinegar liquid: the part by weight of mixing Chinese herbal medicine=3:1, the mixing Chinese herbal medicine 1. prepared with vinegar liquid soaking step, soaks 150-160 hour, crosses and filters the dregs of a decoction, gets its filtrate dilution 10-20 and is doubly described Chinese herbal medicine dilution;
(3) by weight percentage, extracting degreasing degelatinized bone meal 1%-20%, is mixed into compound by above-mentioned plant charcoal 30%-80% and degreasing degelatinized bone meal 1%-20%;
(4) ratio being 10%-50% by weight percentage Chinese herbal medicine dilution prepared by step (2) again adds in the compound of step (3), stirs, packs, and obtains described a kind of plant resource preparation preventing and treating root-knot nematode.
2, pseudo-ginseng field experiment
Below test is conventional method (notoginseng planting and contrast thereof are conventional method) without special instruction.
In same cool canopy, carry out same field comparative trial, process of the present invention and control treatment are set.
The plant resource preparation of the control root-knot nematode that process of the present invention is used, is made up of plant charcoal 80%, Chinese herbal medicine dilution 19% and degreasing degelatinized bone meal 1% by weight percentage; Described Chinese herbal medicine dilution is by vinegar liquid: the part by weight of mixing Chinese herbal medicine=3:1, with vinegar immersion bubble mixing Chinese herbal medicine after 155 hours, crosses and filters the dregs of a decoction, gets its filtrate and dilutes 15 times and obtain; Described mixing Chinese herbal medicine by percentage by weight and component be Aztec marigold 10%, the Chinese herbal medicine of barberry 10%, sheep hoof 40%, climbing groundsel 30%, smilax 10% forms, and its Chinese herbal medicine is cut into the mixing Chinese herbal medicine that pure block is mixed into.
The preparation of the plant resource preparation of the control root-knot nematode that process of the present invention is used by weight percentage, plant charcoal is 80%, Chinese herbal medicine dilution is 19%, degreasing degelatinized bone meal be 1% ratio and following steps preparation;
(1) get plant charcoal, and plant charcoal was shattered 2 mm sieve;
(2) prepare Chinese herbal medicine dilution, be 1. Aztec marigold 10% with component by weight percentage, the Chinese herbal medicine of barberry 10%, sheep hoof 40%, climbing groundsel 30%, smilax 10% is cut into pure block respectively, and is mixed with into mixing Chinese herbal medicine; 2. press vinegar liquid: the part by weight of mixing Chinese herbal medicine=3:1, the mixing Chinese herbal medicine 1. prepared with vinegar liquid soaking step, soaks 155 hours, and mistake filters the dregs of a decoction, gets its filtrate and dilutes 15 times and be described Chinese herbal medicine dilution;
(3) plant charcoal that extracting degreasing degelatinized bone meal and step (1) obtain is mixed into compound;
(4) again Chinese herbal medicine dilution prepared by step (2) is added in the compound of step (3), stir, pack, obtain the plant resource preparation of process of the present invention control root-knot nematode used.
The field practice step of process of the present invention is:
(1), when transplanting panax notoginseng bar, moisture in the soil face is spread fertilizer over the fields the plant resource preparation of process of the present invention control root-knot nematode used, and consumption is 80kg/ mu;
(2), after group bar is emerged, impose the plant resource preparation of process of the present invention control root-knot nematode used respectively in leaf stretching period and green sub-phase in plantation pool soil table once, each consumption is 80kg/ mu;
(3) Second Year: impose the plant resource preparation of process of the present invention control root-knot nematode used respectively in pseudo-ginseng leaf stretching period and green sub-phase in plantation pool soil table once, each consumption is 80kg/ mu.
With conventional control method for contrast: namely during transplanting panax notoginseng bar, sub-bar seedling mixed by 200ppm agricultural streptomycin+25% Yekuling+58% metalaxyl-mn-zn+50% carbendazim+64% Sandofan pulvis, Zhan Yehou of emerging at least monthly uses the bactericide mixed atomizing such as carbendazim, metalaxyl, tmtd, zineb, Yekuling, bacillus subtilis, and Second Year Zhan Yehou of emerging also in kind processes.
Above-mentioned plot area is unit with control (often control area 2.0 × 2.7m, plant 150 seedling panax notoginseng bars), amounts to investigation 10 and controls.Investigation pseudo-ginseng root-rot incidence before pseudo-ginseng results.The same conventional method of the management such as water, fertilizer of Panax notoginseng Growth phase.Result of the test is in table 1.
Notoginseng root rot incidence after the plant resource preparation land for growing field crops application of the control root-knot nematode described in table 1
Note: namely percent of loss equals diseased plant rate.
Table 1 result shows: in same notoginseng planting canopy, conventional control method survives strain rate average out to 58.2%, notoginseng root rot average onset strain rate 41.8%, namely pseudo-ginseng percent of loss is 41.8%, by average yield per mu 200 kilograms calculation, every mu of loss pseudo-ginseng 83.6 kilograms, calculates with nearly 2 years pseudo-ginseng prices 800 yuan/per kilogram, adds up to average loss: 66880 yuan/mu; The inventive method survives strain rate average out to 92.7%, and notoginseng root rot average onset strain rate is 7.267%, and namely pseudo-ginseng percent of loss is 7.267%, every mu of average loss pseudo-ginseng 14.5 kilograms, adds up to average loss: 11627 yuan/mu.The inventive method is than the notoginseng root rot diseased plant rate decreased average 82.6% of contrast, and decreased average loses 55253 yuan/mu, i.e. decreased average economic loss 82.6%, creates unforeseeable technique effect.
The plant resource preparation of described control root-knot nematode is Eco-friendly product; be applied to the control of notoginseng root rot; belong to ecological management plant pest category; have pollution-free, without residual hazard, non-killed natural enemies, do not develop immunity to drugs; be conducive to the advantage such as person poultry safety and environmental protection, cost is low, double prevents double controlling.

Claims (2)

1. prevent and treat the application of plant resource preparation in control notoginseng root rot for root-knot nematode, the plant resource preparation of described control root-knot nematode is by following component and percentage by weight composition: plant charcoal 30%-80%, Chinese herbal medicine dilution 10%-50%, degreasing degelatinized bone meal 1%-20%; Described Chinese herbal medicine dilution is by vinegar liquid: mixing Chinese herbal medicine is the part by weight of 3:1, after 150-160 hour, crosses and filters the dregs of a decoction, get its filtrate dilution 10-20 and doubly obtain with vinegar immersion bubble mixing Chinese herbal medicine; Described mixing Chinese herbal medicine by percentage by weight and component be Aztec marigold 10%-40%, the Chinese herbal medicine of barberry 10%-40%, sheep hoof 10%-40%, climbing groundsel 10%-30%, smilax 10%-40% forms, and its Chinese herbal medicine is cut into pure block or shatters into the mixing Chinese herbal medicine that chaff shape is mixed into.
2. application according to claim 1, is characterized in that carrying out according to the following steps:
(1), when transplanting panax notoginseng bar, moisture in the soil face is spread fertilizer over the fields the plant resource preparation of described control root-knot nematode, and consumption is 80kg/ mu;
(2), after group bar is emerged, the plant resource imposing described control root-knot nematode in leaf stretching period and green sub-phase is respectively formed on plantation pool soil table once, and each consumption is 80kg/ mu;
(3) Second Year: impose the plant resource preparation of described control root-knot nematode respectively in pseudo-ginseng leaf stretching period and green sub-phase in plantation pool soil table once, each consumption is 80kg/ mu.
CN201310289581.0A 2013-07-10 2013-07-10 A kind of application of plant resource preparation in control notoginseng root rot preventing and treating root-knot nematode Active CN103314814B (en)

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CN104642399B (en) * 2015-01-23 2017-11-17 广西大学 The purposes of southern biennial wormwood extract preventing and treating notoginseng root rot
CN105265052A (en) * 2015-09-18 2016-01-27 昆明线敌生物科技有限公司 Biological control method for soft rot of amorphophallus
CN105594771A (en) * 2016-02-22 2016-05-25 云南笃行生物科技有限公司 Pseudo-ginseng root rot prevention and control preparation
CN106034438B (en) * 2016-05-27 2018-02-06 靖西地源生物科技有限公司 A kind of method for preventing and treating pseudo-ginseng disease
CN111512915A (en) * 2020-04-30 2020-08-11 西南林业大学 Seedling growing method for preventing and treating pseudo-ginseng root knot nematode disease

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