CN103238633A - Prevention solution for preharvest disease of Huping jujube and application thereof - Google Patents
Prevention solution for preharvest disease of Huping jujube and application thereof Download PDFInfo
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- CN103238633A CN103238633A CN201310183467XA CN201310183467A CN103238633A CN 103238633 A CN103238633 A CN 103238633A CN 201310183467X A CN201310183467X A CN 201310183467XA CN 201310183467 A CN201310183467 A CN 201310183467A CN 103238633 A CN103238633 A CN 103238633A
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Abstract
The invention discloses a prevention solution for preharvest disease of Huping jujube and application thereof and discloses a fruit disease prevention solution. The provided fruit disease prevention solution contains mineral elements, chitosan and cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner), wherein the mineral elements comprises the elements of calcium, boron, zinc and silicon; the ratio of the calcium to the boron to the zinc to the silicon to the chitosan to the cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is (18.3-36.7g):(1.4-7.1g):(2.3-4.5g):(0.99-4.9g):(50-200g):(1*10<10>-1*10<12>CFU); and the calcium comes from organic calcium. According to the prevention solution, the amount of chemical pesticide and residual hazard of the pesticide on the fruits thereof are reduced, the prevention solution has the obvious effect of preventing the preharvest diseases of Huping jujube, and compared with the control group, the preharvest disease rate of Huping jujube can be reduced by 69.9 percent, and the prevention solution is convenient to use.
Description
Technical field
The present invention relates to a kind of fruit disease control liquid, control liquid and the application thereof of disease before particularly a kind of tall bottle with spout jujube is adopted.
Background technology
The tall bottle with spout jujube is one of China's ten your name's jujubes, in Shanxi, there is establishing in large scale on ground such as Shaanxi, Xinjiang, occupies important status in local peasant's agricultural production and life.Only the area under cultivation of Jinzhong Prefecture, Shanxi tall bottle with spout jujube just surpasses 1,000,000 mu, is the column support type industry of local agro based economic development.Yet in recent years, disease was on the rise before the tall bottle with spout date fruit was adopted, and had caused large tracts of land loss and the underproduction, had extremely seriously influenced the development of tall bottle with spout jujube industry.Disease mainly showed as fruit top or dark red scab in irregular shape appears in umbilical region before the tall bottle with spout date fruit was adopted, scab just is that yellow or pale red, edge are irregular, after be expanded into ellipse or circular dark red scab, the downright bad browning of sick pericarp undertissue, go deep into pulp, flavor is extremely bitter.Morbidity later stage fruit site of pathological change is organized gradually and is rotted.
At present, the orchard worker mainly by spray various chemical pesticides to the tall bottle with spout jujube adopt before disease prevent and treat, but protection effect is very undesirable.Opinions vary for the cause of disease of this disease, by system investigation and the research to disease pests occurrence rule and phenotype, the microorganism that finds the cause of disease not is the principal element of bringing out this disease, behind healthy date fruit, can not cause similar symptom because separate the various pathogen tiebacks that obtain from date fruit, and incidence tissue directly is inoculated in healthy pulp, fruit is not also fallen ill; It is cotton-shaped that incidence tissue becomes, similar to observed physiological disturbance on other fruits.Infecting of date fruit morbidity back pathogenic microorganism is to increase the weight of the main cause that disease takes place.If will effectively prevent and treat the tall bottle with spout jujube adopts preceding disease, need take all factors into consideration the various factors that causes this disease.
Summary of the invention
An object of the present invention is to provide a kind of fruit disease control liquid.
Fruit disease control liquid provided by the present invention contains mineral element, shitosan and Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner); Described mineral element is calcium constituent, boron element, zinc element and element silicon; The proportioning of described calcium constituent, described boron element, described zinc element, described element silicon, described shitosan and described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is 18.3～36.7g:1.4～7.1g:2.3～4.5g:0.99～4.9g:50～200g:1 * 10
10～1 * 10
12CFU; Described calcium constituent derives from organic calcium.
In the present invention, described organic calcium is specially calcium lactate; Described boron element derives from potassium borate; Described zinc element derives from zinc sulphate; Described element silicon derives from sodium silicate;
In described control liquid, the proportioning of described calcium lactate, described potassium borate, described zinc sulphate, described sodium silicate, described shitosan and described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) can be 100～200g:10～50g:10～20g:10～50g:50～200g:1 * 10
10～1 * 10
12CFU is as 200g:50g:10～20g:20～50g:100～200g:1 * 10
11～1 * 10
12CFU is as 200g:50g:20g:30～50g:100～200g:1 * 10
12CFU, 200g:50g:20g:50g:200g:1 * 10 for another example
12CFU.
In described control liquid, also contain the medium of water and the described Cryptococcus laurentii of cultivation (Cryptococcus laurentii (Kuffer.) Skinner).
More concrete, the described control liquid of every 1L composed as follows: 10～20g(is as 10g or 20g) as described in calcium lactate, 1～5g(as 1g or 5g) as described in potassium borate, 1～2g(as 1g or 2g) as described in zinc sulphate, 1～5g(as 1g, 2g, 3g or 5g) as described in sodium silicate, 5～20g(as 5g, 10g or 20g) as described in shitosan, 1 * 10
9～1 * 10
11CFU(is as 1 * 10
9CFU, 1 * 10
10CFU or 1 * 10
11CFU) described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner), surplus are that volume ratio is the water of 1:1 and the medium of cultivating described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner).
The solvent of the medium of described cultivation Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is water, and solute and concentration are as follows: yeast extract 10g/L, peptone 20g/L, glucose 20g/L, pH5.8.
In the present invention, described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is specially Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029.
Another object of the present invention provides the preparation method of described control liquid.
The preparation method of described control liquid can comprise the steps:
(1) calcium lactate, potassium borate, zinc sulphate, sodium silicate, shitosan and water are mixed according to the ratio of 100～200g:10～50g:10～20g:10～50g:50～200g:5L, preparation obtains solution I;
(2) get and the isopyknic Cryptococcus laurentii of water described in the step (1) (Cryptococcus laurentii (Kuffer.) Skinner) bacteria suspension, mix with the described solution I that step (1) obtains, make that Cryptococcus laurentii described in the mixed liquor (Cryptococcus laurentii (Kuffer.) Skinner) and the ratio of described calcium lactate are 1 * 10
10～1 * 10
12CFU:100～200g, gained mixed liquor are described control liquid.
In the present invention, the preparation method of described control liquid specifically comprises the steps:
(a1) with 100～200g(such as 100g or 200g) calcium lactate, 10～50g(such as 10g or 50g) potassium borate, 10～20g(such as 10g or 20g) zinc sulphate, 10～50g(such as 10g, 20g, 30g or 50g) sodium silicate, 50～200g(such as 50g, 100g or 200g) shitosan is dissolved in the 5L water, obtains solution I;
(a2) getting the 5L bacterial content is 2 * 10
6～2 * 10
8CFU/mL(is as 2 * 10
6CFU/mL, 2 * 10
7CFU/mL or 2 * 10
8CFU/mL) the described solution I that Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) bacteria suspension and step (a1) obtain is mixed, and obtains described control liquid.
In said method, the bacteria suspension of described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) bacteria suspension for obtaining after described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) being placed following medium cultivate: solvent is water, solute and concentration are as follows: yeast extract 10g/L, peptone 20g/L, glucose 20g/L, pH5.8.
In said method, described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is specially Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029.
The application in the disease before control tall bottle with spout jujube is adopted of described control liquid also belongs to protection scope of the present invention.
Another purpose of the present invention provides a kind of method that the tall bottle with spout jujube is adopted preceding disease of preventing and treating.
The method of disease before control tall bottle with spout jujube provided by the present invention is adopted is to begin from tall bottle with spout jujube young fruit period or expanding stage, regularly described control liquid is directly sprayed on the fruit and blade of described tall bottle with spout jujube, when gathering preceding 3 weeks only.
In said method, it is 0.5-1.0cm(such as 0.5cm that described young fruit period can be described tall bottle with spout date fruit major diameter) time; Described expanding stage is that described tall bottle with spout date fruit major diameter is 2-3cm(such as 3cm) time.
In said method, the described frequency that sprays be 2-4 week/time (as 2 the week/time or 4 the week/time).
In said method, the described dosage that sprays is that every tree sprays 2L at every turn.
In the present invention, described shitosan is that molecular weight is the shitosan of 350kDa.In one embodiment of the invention, described shitosan is specially Qingdao Honghai Biotechnology Co., Ltd.'s product, and its catalog number is: YZ080115.
In the present invention, the disease of disease is characterized as before described disease or described the adopting: the dark red scab appears in fruit top or umbilical region.
More concrete, described disease is characterized as: dark red scab in irregular shape appears in fruit top or umbilical region, and scab just is that yellow or pale red, edge are irregular, after be expanded into ellipse or circular dark red scab, the downright bad browning of sick pericarp undertissue gos deep into pulp, and flavor is extremely bitter; Morbidity later stage fruit site of pathological change tissue rots.
Fruit disease control liquid provided by the present invention, wherein calcium lactate, potassium borate and zinc sulphate can effectively replenish the date fruit required mineral element that grows; Calcium lactate is organic calcium, plant is not produced poisoning; Sodium silicate can improve self disease resistance of fruit; Shitosan can form diaphragm at fruit surface as fruit glaze agent, hinders infecting of pathogenic microorganism; And Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) can effectively reduce the superinfection of pathogenic microorganism as the biological control saccharomycete, plays the effect of integrated control.Experiment showed, with control liquid control tall bottle with spout jujube provided by the present invention and adopt preceding disease effect significantly (compare with control group, the disease rate reduces by 69.9% before the tall bottle with spout jujube can being adopted), and easy to use.The present invention is under the situation of not using chemical pesticide; comprehensive mineral element and the antagonist of using; can effectively prevent and treat the tall bottle with spout jujube and adopt preceding disease, reduce the consumption of chemical pesticide and the residual hazard on fruit thereof, play protection health and antipollution effect.
Embodiment
Employed experimental technique is conventional method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Calcium lactate (Xilong Chemical Co., Ltd; Catalog number: 814-80-2), potassium borate (Chemical Reagent Co., Ltd., Sinopharm Group; Catalog number: 12045-78-2), zinc sulphate (Xilong Chemical Co., Ltd; Catalog number: 7446-20-0), sodium silicate (Xilong Chemical Co., Ltd; Catalog number: 13517-24-3).
Shitosan: molecular formula is [C6H13NO5] n, and molecular weight is 350kDa, is Qingdao Honghai Biotechnology Co., Ltd.'s product, and its catalog number is: YZ080115.
Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3 bacterial strain: this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on November 12nd, 2003 and (is called for short CGMCC, the address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City), the preservation center numbering of registering on the books: CGMCC No.1029.This bacterial strain has been on November 19th, 2003 in the applying date, and application number is to disclose in 200310115336.4 the patent application, and patent publication No. is CN1544614A.
The Yeast Cultivation base: solvent is water, and solute and concentration are as follows: yeast extract 10g/L, peptone 20g/L, glucose 20g/L, pH5.8.Wherein, yeast extract is that Beijing bispin microorganism is cultivated company's product, and its catalog number is CM02-12A; Peptone Beijing bispin microorganism is cultivated company's product, and its catalog number is CM02-31.
Each test among the following embodiment is all carried out in experiment orchard, Shanxi province Taiyuan city Qingxu County.In the test according to following criterion, assert whether fruit is the disease fruit: sick fruit shows as: dark red scab in irregular shape appears in fruit top or umbilical region, scab just is that yellow or pale red, edge are irregular, after be expanded into ellipse or circular dark red scab, the downright bad browning of sick pericarp undertissue, go deep into pulp, flavor is extremely bitter; Morbidity later stage fruit site of pathological change is organized gradually and is rotted.
Embodiment 1:
One, the preparation of fruit disease control liquid
1, with Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029, is inoculated in the Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hour time, obtain seed liquor; Concentration with Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 described in the whole described seed liquor of described Yeast Cultivation keynote is 2 * 10
6CFU/mL obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 100g, potassium borate 10g, zinc sulphate 10g, sodium silicate 10g, shitosan 50g and water 5L being mixed, be stirred to dissolving fully, is 2 * 10 with 5L bacterial strain concentration then
6The Cryptococcus laurentii of CFU/mL (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension mixes, and stirs, and obtains fruit disease control liquid.
Two, the control of disease before the tall bottle with spout jujube is adopted
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter) beginning, the control liquid every 2 weeks with the step 1 preparation directly sprays on tall bottle with spout date fruit and blade, ends when gathering preceding 3 weeks, and spraying dosage is that each 2 liters of control liquid (experimental group) are set in every strain.Every processing 10 strain jujube trees are to spray clear water in contrast.Before fruit maturation was plucked, the tall bottle with spout date fruit of statistical experiment group and control group was adopted preceding diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity * 100%.
The result shows, diseased fruit rate was 51.8% before the experimental group tall bottle with spout date fruit that sprays control liquid was adopted, and the control group diseased fruit rate that sprays clear water is 85.2%, and diseased fruit rate has reduced 33.4% before adding up to experimental group to compare with control group to adopt.
Embodiment 2:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in the Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hour time, obtain seed liquor; Concentration with Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 described in the whole described seed liquor of described Yeast Cultivation keynote is 2 * 10
7CFU/mL obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 10g, sodium silicate 20g, shitosan 100g and water 5L being mixed, be stirred to dissolving fully, is 2 * 10 with 5L bacterial strain concentration then
7The Cryptococcus laurentii of CFU/mL (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension mixes, and stirs, and obtains fruit disease control liquid.
Two, the control of disease before the tall bottle with spout jujube is adopted
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter) beginning, the control liquid every 2 weeks with the step 1 preparation directly sprays on tall bottle with spout date fruit and blade, ends when gathering preceding 3 weeks, and spraying dosage is that each 2 liters of control liquid (experimental group) are set in every strain.Every processing 10 strain jujube trees are to spray clear water in contrast.Before fruit maturation was plucked, the tall bottle with spout date fruit of statistical experiment group and control group was adopted preceding diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity * 100%.
The result shows, diseased fruit rate was 25.6% before the experimental group tall bottle with spout date fruit that sprays control liquid was adopted, and the control group diseased fruit rate that sprays clear water is 85.2%, and diseased fruit rate has reduced 59.6% before adding up to experimental group to compare with control group to adopt.
Embodiment 3:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in the Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hour time, obtain seed liquor; Concentration with Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 described in the whole described seed liquor of described Yeast Cultivation keynote is 2 * 10
8CFU/mL obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 20g, sodium silicate 30g, shitosan 100g and water 5L being mixed, be stirred to dissolving fully, is 2 * 10 with 5L bacterial strain concentration then
8The Cryptococcus laurentii of CFU/mL (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension mixes, and stirs, and obtains fruit disease control liquid.
Two, the control of disease before the tall bottle with spout jujube is adopted
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter) beginning, the control liquid every 2 weeks with the step 1 preparation directly sprays on tall bottle with spout date fruit and blade, ends when gathering preceding 3 weeks, and spraying dosage is that each 2 liters of control liquid (experimental group) are set in every strain.Every processing 10 strain jujube trees are to spray clear water in contrast.The tall bottle with spout date fruit of statistical experiment group and control group is adopted preceding diseased fruit rate before fruit maturation is plucked.Diseased fruit rate=morbidity fruit quantity/fruit total quantity * 100%.
The result shows, diseased fruit rate was 17.8% before the experimental group tall bottle with spout date fruit that sprays control liquid was adopted, and the control group diseased fruit rate that sprays clear water is 85.2%, and diseased fruit rate has reduced 67.4% before adding up to experimental group to compare with control group to adopt.
Embodiment 4:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in the Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hour time, obtain seed liquor; Concentration with Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 described in the whole described seed liquor of described Yeast Cultivation keynote is 2 * 10
8CFU/mL obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 20g, sodium silicate 50g, shitosan 200g and water 5L being mixed, be stirred to dissolving fully, is 2 * 10 with 5L bacterial strain concentration then
8The Cryptococcus laurentii of CFU/mL (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension mixes, and stirs, and obtains fruit disease control liquid.
Two, the control of disease before the tall bottle with spout jujube is adopted
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter) beginning, the control liquid every 2 weeks with the step 1 preparation directly sprays on tall bottle with spout date fruit and blade, ends when gathering preceding 3 weeks, and spraying dosage is that each 2 liters of control liquid (experimental group) are set in every strain.Every processing 10 strain jujube trees are to spray clear water in contrast.Before fruit maturation was plucked, the tall bottle with spout date fruit of statistical experiment group and control group was adopted preceding diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity * 100%.
The result shows, diseased fruit rate was 15.3% before the experimental group tall bottle with spout date fruit that sprays control liquid was adopted, and the control group diseased fruit rate that sprays clear water is 85.2%, and diseased fruit rate has reduced 69.9% before adding up to experimental group to compare with control group to adopt.
Embodiment 5:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in the Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hour time, obtain seed liquor; Concentration with Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 described in the whole described seed liquor of described Yeast Cultivation keynote is 2 * 10
8CFU/mL obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 20g, sodium silicate 50g, shitosan 200g and water 5L being mixed, be stirred to dissolving fully, is 2 * 10 with 5L bacterial strain concentration then
8The Cryptococcus laurentii of CFU/mL (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension mixes, and stirs, and obtains fruit disease control liquid.
Two, the control of disease before the tall bottle with spout jujube is adopted
From tall bottle with spout date fruit young fruit period (0.5 centimetre of fruit major diameter) beginning, the control liquid every 4 weeks with the step 1 preparation directly sprays on tall bottle with spout date fruit and blade, ends when gathering preceding 3 weeks, and spraying dosage is that each 2 liters of control liquid (experimental group) are set in every strain.Every processing 10 strain jujube trees are to spray clear water in contrast.Before fruit maturation was plucked, the tall bottle with spout date fruit of statistical experiment group and control group was adopted preceding diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity * 100%.
The result shows, diseased fruit rate was 49.5% before the experimental group tall bottle with spout date fruit that sprays control liquid was adopted, and the control group diseased fruit rate that sprays clear water is 85.2%, and diseased fruit rate has reduced 35.7% before adding up to experimental group to compare with control group to adopt.
Embodiment 6:
One, the preparation of fruit disease control liquid
1, Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 is inoculated in the Yeast Cultivation base, under 28 ° of C conditions, cultivates 36 hour time, obtain seed liquor; Concentration with Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 described in the whole described seed liquor of described Yeast Cultivation keynote is 2 * 10
8CFU/mL obtains Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension.
2, calcium lactate 200g, potassium borate 50g, zinc sulphate 20g, sodium silicate 50g, shitosan 200g and water 5L being mixed, be stirred to dissolving fully, is 2 * 10 with 5L bacterial strain concentration then
8The Cryptococcus laurentii of CFU/mL (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029 bacteria suspension mixes, and stirs, and obtains fruit disease control liquid.
Two, the control of disease before the tall bottle with spout jujube is adopted
From tall bottle with spout date fruit expanding stage (3 centimetres of fruit major diameters) beginning, the control liquid every 2 weeks with the step 1 preparation directly sprays on tall bottle with spout date fruit and blade, ends when gathering preceding 3 weeks, and spraying dosage is that each 2 liters of control liquid (experimental group) are set in every strain.Every processing 10 strain jujube trees are to spray clear water in contrast.Before fruit maturation was plucked, the tall bottle with spout date fruit of statistical experiment group and control group was adopted preceding diseased fruit rate.Diseased fruit rate=morbidity fruit quantity/fruit total quantity * 100%.
The result shows, diseased fruit rate was 56.7% before the experimental group tall bottle with spout date fruit that sprays control liquid was adopted, and the control group diseased fruit rate that sprays clear water is 85.2%, and diseased fruit rate has reduced 28.5% before adding up to experimental group to compare with control group to adopt.
The result of comprehensive above each embodiment, as can be seen, the effect of disease before using fruit control liquid control tall bottle with spout date fruit provided by the present invention to adopt, with mineral element, fruit glaze agent, the saccharomycetic working concentration of biological control in the control liquid, fruit developmental stage when control liquid uses, use blanking time etc. relevant, with embodiment 4 best results, with control group phase specific energy the tall bottle with spout date fruit is adopted preceding disease rate and reduce by 69.9%.
Claims (9)
1. a fruit disease control liquid contains mineral element, shitosan and Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner); Described mineral element is calcium constituent, boron element, zinc element and element silicon; The proportioning of described calcium constituent, described boron element, described zinc element, described element silicon, described shitosan and described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is 18.3～36.7g:1.4～7.1g:2.3～4.5g:0.99～4.9g:50～200g:1 * 10
10～1 * 10
12CFU; Described calcium constituent derives from organic calcium.
2. control liquid according to claim 1, it is characterized in that: described organic calcium is calcium lactate; Described boron element derives from potassium borate; Described zinc element derives from zinc sulphate; Described element silicon derives from sodium silicate;
In described control liquid, the proportioning of described calcium lactate, described potassium borate, described zinc sulphate, described sodium silicate, described shitosan and described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is 100～200g:10～50g:10～20g:10～50g:50～200g:1 * 10
10～1 * 10
12CFU.
3. the preparation method of the described control liquid of claim 2 comprises the steps:
(1) calcium lactate, potassium borate, zinc sulphate, sodium silicate, shitosan and water are mixed according to the ratio of 100～200g:10～50g:10～20g:10～50g:50～200g:5L, preparation obtains solution I;
(2) get and the isopyknic Cryptococcus laurentii of water described in the step (1) (Cryptococcus laurentii (Kuffer.) Skinner) bacteria suspension, mix with the described solution I that step (1) obtains, make that Cryptococcus laurentii described in the mixed liquor (Cryptococcus laurentii (Kuffer.) Skinner) and the ratio of described calcium lactate are 1 * 10
10～1 * 10
12CFU:100～200g, gained mixed liquor are the described control liquid of claim 2.
4. control liquid according to claim 1 and 2, or the described method of claim 3, it is characterized in that: described Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) is Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC No.1029.
5. claim 1 or the 2 described control liquid application in the disease before control tall bottle with spout jujube is adopted.
6. the method for disease was to begin from tall bottle with spout jujube young fruit period or expanding stage before control tall bottle with spout jujube was adopted, regularly claim 1 or 2 described control liquid are directly sprayed on the fruit and blade of described tall bottle with spout jujube, up to gather preceding 3 when all till.
7. method according to claim 6 is characterized in that: in said method, described young fruit period is that described tall bottle with spout date fruit major diameter is when being 0.5-1.0cm; Described expanding stage is that described tall bottle with spout date fruit major diameter is when being 2.0-3.0cm.
8. according to claim 6 or 7 described methods, it is characterized in that: the described frequency that sprays be 2-4 week/time.
9. according to arbitrary described method among the claim 6-8, it is characterized in that: the described dosage that sprays is that each 2 liters of described control liquid are set in every strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310183467.XA CN103238633B (en) | 2013-05-17 | 2013-05-17 | Prevention solution for preharvest disease of Huping jujube and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310183467.XA CN103238633B (en) | 2013-05-17 | 2013-05-17 | Prevention solution for preharvest disease of Huping jujube and application thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN103238633A true CN103238633A (en) | 2013-08-14 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105660267A (en) * | 2016-03-21 | 2016-06-15 | 贵州大学 | Novel method and protective film agent for preventing and controlling soft rot of actinidia and preparation method for protective film agent |
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| CN1543796A (en) * | 2003-11-19 | 2004-11-10 | 中国科学院植物研究所 | Method for preventing and treating postharvest disease of fruit and vegetable using sodium silicate or potassium silicate and liquid for preventing and treating postharvest disease of fruit and vegeta |
| CN1544614A (en) * | 2003-11-19 | 2004-11-10 | 中国科学院植物研究所 | Bacteria for biocontrol of disease and its application |
| CN101491273A (en) * | 2009-03-04 | 2009-07-29 | 中国科学院植物研究所 | Grape postharvest diseases or fruit abscission prevention and control method and special prevention and control agent |
| CN101914459A (en) * | 2010-04-20 | 2010-12-15 | 浙江大学 | Peach fruit disease biological antiseptic preservative and application and used cryptococcus laurentii |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1543796A (en) * | 2003-11-19 | 2004-11-10 | 中国科学院植物研究所 | Method for preventing and treating postharvest disease of fruit and vegetable using sodium silicate or potassium silicate and liquid for preventing and treating postharvest disease of fruit and vegeta |
| CN1544614A (en) * | 2003-11-19 | 2004-11-10 | 中国科学院植物研究所 | Bacteria for biocontrol of disease and its application |
| CN101491273A (en) * | 2009-03-04 | 2009-07-29 | 中国科学院植物研究所 | Grape postharvest diseases or fruit abscission prevention and control method and special prevention and control agent |
| CN101914459A (en) * | 2010-04-20 | 2010-12-15 | 浙江大学 | Peach fruit disease biological antiseptic preservative and application and used cryptococcus laurentii |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660267A (en) * | 2016-03-21 | 2016-06-15 | 贵州大学 | Novel method and protective film agent for preventing and controlling soft rot of actinidia and preparation method for protective film agent |
| CN105660267B (en) * | 2016-03-21 | 2019-03-29 | 贵州大学 | The new method of prevention and control Kiwi berry soft rot and protection film and preparation method thereof |
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