CN101416642A - Fruit-tree aphides and red mites prevention and treatment preparation and production method and use method thereof - Google Patents

Fruit-tree aphides and red mites prevention and treatment preparation and production method and use method thereof Download PDF

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CN101416642A
CN101416642A CNA2008103054741A CN200810305474A CN101416642A CN 101416642 A CN101416642 A CN 101416642A CN A2008103054741 A CNA2008103054741 A CN A2008103054741A CN 200810305474 A CN200810305474 A CN 200810305474A CN 101416642 A CN101416642 A CN 101416642A
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urine
preparation
milliliters
synergist
extract
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CN101416642B (en
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阿地力·沙塔尔
潘存德
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Xinjiang Agricultural University
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Xinjiang Agricultural University
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention relates to the technical field against forest and fruit tree pest, particularly to a preparation against aphis of fruit trees and red mite, preparing method and application thereof. The preparation against aphis of fruit trees and red mite is bovine urine. The bovine urine may be raw bovine urine which is unfermented bovine urine, fermented bovine urine, supernatant of fermented bovine urine, deposited liquid of bovine urine, or composite preparation of bovine urine. The raw materials of invention can be obtained easily with low cast and the technical processe is easily operated. Using the preparation can effectively prevent and treat different kinds of red mites and aphis on different kinds of fruit trees, flowers, and vegetables. The invention causes little pollution to the environment and no drug resistance, in addition, the invention will not kill natural enemy of pest by mistake.

Description

Control fruit aphid and two spotted spider mite preparation and production method and using method
One, technical field
The present invention relates to the Prevention Technique of woods fruit insect, is a kind of control fruit aphid and two spotted spider mite preparation and production method and using method.
Two, background technology
Industry of planting forest or fruit tress has obtained unprecedented attention, especially economic forest and has planted the rapid expansion of planting area in Xinjiang in social economy's sustainable development, economy, ecology and social benefit are obvious day by day.But the insect pest of woods fruit disease seriously affects the performance of the quality and the function of industry of planting forest or fruit tress, has become the sound development one big obstacle of restriction woods fruit industry and ecological two big systems.
At present, the loss that Xinjiang fruit tree primary pest is caused is everlasting more than 20%, some areas and time even reach more than 90%.According to Xinjiang forestry department investigation statistics in 2007, area takes place woods fruit pest is 5,800,000 mu, and the area that causes disaster reaches 2,300,000 mu.Area takes place piercing mouth parts such as aphid, two spotted spider mite class pest is 2,750,000 mu, and the disaster area reaches 1,230,000 mu.Advance in the past few years sucking pest such as two spotted spider mite in the harm aggravation of area, South Sinkiang, it is wider that damaging range takes place, be injured the heaviest with seeds such as walnut, red date, apple, Ba Danmu, apricot, pomegranates, red date, apricot are endangered by two spotted spider mite, cause less than the fructescence blade and come off with regard to having turned to be yellow, fruit dries up, comes off, part apricot, almond, red date garden 8, September the blade light that falls, the normal growth that has a strong impact on fruit tree is grown.For a long time because a large amount of chemical pesticides that use spectrum, caused environmental pollution, insect to develop immunity to drugs and manslaughtered problem such as pest natural enemy.
Three, summary of the invention
The invention provides a kind of control fruit aphid and two spotted spider mite preparation and production method and using method, the defective that it has solved effectively that the prior art environmental pollution is serious, insect easily develops immunity to drugs etc., it is a raw material with one of ox urine or ox urine, environment is not polluted, do not manslaughter pest natural enemy, insect effects such as control fruit aphid and two spotted spider mite are better.
One of technical scheme of the present invention is achieved in that a kind of control fruit aphid and two spotted spider mite preparation, and its this control fruit aphid and two spotted spider mite preparation are the ox urine.
Above-mentioned ox urine is an ox urine zymotic fluid, and it obtains as follows: with the filtration of ox urine, the decontamination of gathering, in the transparent container of packing into, be to ferment 15 to 30 days between 25 to 30 degrees centigrade in temperature.
Above-mentioned ox urine obtains as follows: with the filtration of ox urine, the decontamination of gathering, pack in the transparent container, in temperature is to ferment 15 to 30 days between 25 to 30 degrees centigrade, after fermentation finishes, in temperature is under 20 degrees centigrade, left the heart 5 minutes with per minute 6000, obtaining ox urine supernatant is the ox urine.
Above-mentioned ox urine obtains as follows: with the filtration of ox urine, the decontamination of gathering, pack in the transparent container, in temperature is to ferment 15 to 30 days between 25 to 30 degrees centigrade, after fermentation finishes, in temperature is under 20 degrees centigrade, left the heart 5 minutes with per minute 6000, obtaining ox urinary precipitation liquid is the ox urine.
Two of technical scheme of the present invention is achieved in that a kind of compound formulation of preventing and treating fruit aphid and two spotted spider mite preparation, and it consists of ox urine and synergist by raw material.
Above-mentioned synergist is a urea, and ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, and the ox urine after per 500 milliliters of dilutions adds 50 gram urea, is mixed with the compound formulation that contains 10% urea;
Above-mentioned synergist is a carboxymethyl cellulose, and ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, and the ox urine after per 500 milliliters of dilutions adds 0.05 gram carboxymethyl cellulose, is mixed with the compound formulation that contains 0.01% carboxymethyl cellulose;
Above-mentioned synergist is one can be good, and ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, and the ox urine after per 500 milliliters of dilutions adds one of 0.5 gram can be good, be mixed with contain 0.1% 1 can be good compound formulation;
Above-mentioned synergist is the leek extract, leek smashed to pieces put into gauze bag and directly get juice and obtain the leek extract, ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, and the ox urine after per 450 milliliters of dilutions adds 50 milliliters of leek extracts, is mixed with the compound formulation that contains 10% leek extract;
Above-mentioned synergist is a ginger extract, ginger smashed to pieces put into gauze bag and directly get juice and obtain ginger extract, ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, and the ox urine after per 450 milliliters of dilutions adds 50 milliliters of ginger extracts, is mixed with the compound formulation that contains 10% ginger extract;
Above-mentioned synergist is a garlic extract, garlic peeling smashed to pieces put into gauze bag and directly get juice and obtain garlic extract, ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, ox urine after per 450 milliliters of dilutions adds 50 milliliters of garlic extracts, is mixed with the compound formulation that contains 10% garlic extract;
Three of technical scheme of the present invention is achieved in that a kind of using method of preventing and treating fruit aphid and two spotted spider mite preparation, aurochs urine or ox are urinated zymotic fluid or ox urine supernatant or ox urinary precipitation liquid to fruit tree sprinkling use, using interval is 3 days, and spray in promptly per 3 days once connects and sprays 3 times.
Four of technical scheme of the present invention is achieved in that a kind of using method of preventing and treating the compound formulation of fruit aphid and two spotted spider mite preparation, compound formulation is sprayed to fruit tree use, and using interval is 3 days, and spray in promptly per 3 days once connects and sprays 3 times.
Raw material of the present invention is easy to obtain, cost is low, technical process is convenient to operation, uses this reagent can prevent and treat the various two spotted spider mite and the aphid of taking place on each planting fruit-trees, flowers and the vegetables effectively, and environmental pollution is few, insect is developed immunity to drugs, nor can manslaughter pest natural enemy.
Four, description of drawings
Accompanying drawing 1 is the control efficiency contrast of ox urine zymotic fluid of the present invention to three kinds of insects.
Accompanying drawing 2 is the control efficiency contrast of the ox urine zymotic fluid of variable concentrations of the present invention to rose aphid.
Accompanying drawing 3 is the influence of ox urine of the present invention to the two spotted spider mite insect density.
Accompanying drawing 4 is the control efficiency of ox urine different disposal of the present invention to two spotted spider mite.
Accompanying drawing 5 is the influence of ox urine of the present invention to the aphid insect density.
Accompanying drawing 6 is the control efficiency of ox urine different disposal of the present invention to aphid.
Accompanying drawing 7 is the control efficiency of six kinds of synergist of the present invention to cinnabar liquid mite.
Accompanying drawing 8 is the control efficiency of ox urine synergist of the present invention to aphid.
Accompanying drawing 9 is the control efficiency of four kinds of synergist of the present invention to Tetranychus cinnabarinus.
Accompanying drawing 10 is the control efficiency of ox urine synergist of the present invention to aphid.
Five, specific embodiment
The present invention is not subjected to the restriction of following embodiment, can determine concrete embodiment according to the technical scheme and the actual conditions of the invention described above.
Embodiment 1, and this control fruit aphid and two spotted spider mite preparation are the ox urine.
Embodiment 2, the ox urine of this control fruit aphid and two spotted spider mite preparation is an ox urine zymotic fluid, this ox urine zymotic fluid obtains as follows: with the filtration of ox urine, the decontamination of gathering, in the transparent container of packing into, be to ferment 15 to 30 days between 25 to 30 degrees centigrade in temperature.
Be that ox urine zymotic fluid is tested two spotted spider mite, aphid isothermal chamber flowers pest controling effect below:
Control efficiency adopts following computational methods:
Control efficiency
P ( % ) = ( T 0 - T n T 0 ) × 100
Corrected mortality
P ′ ( % ) = ( 1 - T n × CK 0 T 0 × CK n ) × 100
Wherein: T 0Insect population quantity T before the expression control nExpression control back insect population quantity
CK 0Insect population quantity CK before handle the expression check plot nInsect population quantity when effect is checked in the expression check plot
Test method: ox is urinated zymotic fluid and water is mixed with solution by the volume ratio of 1:3,1:5,1:7 respectively.By the ox urine zymotic fluid of 1:3 volume ratio dilution on the plant leaf that evenly was sprayed on Tetranychus cinnabarinus harm in three days; By the ox urine zymotic fluid of 1:3,1:5,1:7 volume ratio dilution on the plant leaf that evenly was sprayed on rose aphid harm in three days.More than each is handled and to establish repetition and contrast respectively.Test data is as shown in table 1.
From table 1 and accompanying drawing 1 as can be seen, 1: 3 volume ratio dilution of ox urine zymotic fluid is all fine to the control efficiency of rose aphid and Tetranychus cinnabarinus, has reached 100% respectively at the control efficiency of the 4th day after the dispenser and the 6th day.But then not as the ideal to rose aphid and Tetranychus cinnabarinus, ten days control efficiency just reaches 78.78% to the control efficiency of Trialeurodes vaporariorum Westwood.
By table 2 and accompanying drawing 2 as can be seen, fine to the control efficiency of aphid through ox urine zymotic fluid and water with the solution that the volume ratio of 1:3,1:5 is mixed with, reach 100%, but the ox of 1:3 urine dilution produces to a certain degree poisoning to the flowers blade, i.e. the burnt limit of flowers leaf margin, phenomenon such as withered.
This test supplies 1:3, the 1:5 of examination, ox urine zymotic fluid dilution and the essential wind oil of 1:7 that Tetranychus cinnabarinus, rose aphid are all had good control efficiency.Wherein ox urine zymotic fluid 1:3,1:5 dilution have reached 100% to Tetranychus cinnabarinus, 6 days control efficiency of rose aphid, can also make killed blade return to green, the recovery of the plant that helps to be injured.Essential wind oil has also reached 100% to the control efficiency of rose aphid, Trialeurodes vaporariorum Westwood, and can not produce poisoning to flowers.
In process of the test, though the ox of 1:3 urine zymotic fluid dilution has good control efficiency to aphid, the flowers blade is produced to a certain degree poisoning, i.e. the burnt limit of flowers leaf margin, phenomenon such as withered.Especially show thin blade, or through the blade of sun direct projection, and after being diluted to 1:7 concentration, not only the control efficiency to the plant that is injured reduces, and the death time of insect also prolonged, so suggestion should be determined dilution ratio according to the actual conditions of flowers before using.
Embodiment 3, the ox urine of this control fruit aphid and two spotted spider mite preparation is the ox urine supernatant of ox urine zymotic fluid, this ox urine supernatant obtains as follows: with the filtration of ox urine, the decontamination of gathering, pack in the transparent container, in temperature is to ferment 15 to 30 days between 25 to 30 degrees centigrade, after fermentation finishes, is under 20 degrees centigrade in temperature, left the heart 5 minutes with per minute 6000, obtaining supernatant is ox urine supernatant.
Embodiment 4, the ox urine of this control fruit aphid and two spotted spider mite preparation is the ox urinary precipitation liquid of ox urine zymotic fluid, this ox urinary precipitation liquid obtains as follows: with the filtration of ox urine, the decontamination of gathering, pack in the transparent container, in temperature is to ferment 15 to 30 days between 25 to 30 degrees centigrade, after fermentation finishes, is under 20 degrees centigrade in temperature, left the heart 5 minutes with per minute 6000, obtaining precipitated liquid is ox urinary precipitation liquid.
Be ox urine supernatant, ox urinary precipitation liquid, ox urine zymotic fluid below to two spotted spider mite, the aphid test of pesticide effectiveness:
Control efficiency adopts following computational methods:
Control efficiency
P ( % ) = ( T 0 - T n T 0 ) × 100
Corrected mortality
P ′ ( % ) = ( 1 - T n × CK 0 T 0 × CK n ) × 100
Wherein: T 0: insect population quantity T before the expression control n: expression control back insect population quantity
CK 0: insect population quantity CK before handle the expression check plot n: control back, check plot insect population quantity
Test method: ox urine supernatant, ox urinary precipitation liquid and ox urine zymotic fluid water are respectively pressed the dilution of 1:5 volume ratio, are sprayed onto respectively on the plant corpus of two spotted spider mite, aphid.Each handles 3 repetitions, establishes contrast again.Disposing back was that unit is every 1 insect death toll of 1 day statistics, until the 8th day with the blade since the 2nd day.
Result of the test proves, 3 kinds of ox urines of the examination that supplies to twice control of insects such as aphid, two spotted spider mite after the 7th day control efficiency all reached more than 94%.But ox urine supernatant exceeds about 30% than not centrifugal ox urine zymotic fluid controlling effect the early stage of these 2 kinds of insects in three kinds of ox urines.In addition, 3 kinds of ox urines are to the lasting period of insects such as aphid, two spotted spider mite, the lasting period of ox urine supernatant compared long about 1 day than ox urine zymotic fluid and ox urinary precipitation liquid phase, the 4th day insect population density is continuing decline after the dispenser, and the insect density of urinating zymotic fluid and ox urinary precipitation liquid processed group with ox rose since the 4th day.
By table 3 and accompanying drawing 3 as can be seen, three kinds of ox urines: ox urine zymotic fluid, ox urine supernatant, ox urinary precipitation liquid are in the 2nd day observation, and the two spotted spider mite insect density rate of going down is respectively 37.4%, 41.6%, 60%.The 3rd day the two spotted spider mite insect density rate of going down is respectively 27.9%, 49.6%, 18.5%.The 4th day the two spotted spider mite insect density rate of going down is respectively-22.8%, 9.4% ,-9.5%.This shows that the lasting period of ox urine supernatant urinates zymotic fluid than ox and ox urinary precipitation liquid phase ratio is long, the 4th day insect population density descends lasting after the dispenser, and the insect density of urinating zymotic fluid and ox urinary precipitation liquid processed group with ox was since rising in the 4th day.The insect density of check plot is constantly increasing.
By table 4 and accompanying drawing 4 result of the tests as can be seen, three kinds of ox urines: in ox urine zymotic fluid, ox urine supernatant, the 3rd day the observation of ox urinary precipitation liquid after the reagent first time, the control efficiency of two spotted spider mite is reached for 40.8%, 70.3%, 68.9% respectively.This shows that the control efficiency of ox urine supernatant and ox urinary precipitation liquid is best after the dispenser for the first time, is reached for 70.3%, 68.9% respectively.3 days control efficiency of ox urine zymotic fluid is relatively poor, is 40.8%.After the dispenser for the second time, each control efficiency of handling has all reached more than 94%.The control efficiency of check plot is-77%, and promptly insect density is constantly increasing.
By table 5 and accompanying drawing 5 as can be seen, three kinds of ox urines: ox urine zymotic fluid, ox urine supernatant, ox urinary precipitation liquid are in the time of the 1st day, and the aphid insect density rate of going down is respectively 18.5%, 41.2%, 35.9%.So after the dispenser for the second time, the aphid insect density sharply drops to 18.8%, 34.3%, 32% respectively.The insect population quantity of check plot constantly increases, and rises to 217 (head/leaves) from preventing and treating preceding insect density 193 (head/leaf).This shows that the aphid of the supernatant processed group rate that goes down is higher in the middle of twice dispenser.
By table 6 and accompanying drawing 6 as can be seen, three kinds of ox urines: ox urine zymotic fluid, ox urine supernatant, ox urinary precipitation liquid are after the dispenser first time, and the control efficiency to aphid in the time of the 3rd day is reached for 48.9%, 80.7%, 59.5% respectively.So for the second time after the dispenser, three kinds of ox urines the 4th day control efficiency all reached more than 98%.The control efficiency of check plot is-12%, and promptly insect density is constantly increasing.
Result of the test proves: three kinds of ox urines of the examination that supplies are all desirable to total control efficiency of insects such as aphid, two spotted spider mite, promptly reached the control efficiency more than 94%.But supernatant exceeds about 30% than ox urine zymotic fluid controlling effect the early stage of these 2 kinds of insects in three kinds of treatment fluids.
Three kinds of ox urines are to the lasting period of insects such as aphid, two spotted spider mite, the lasting period of ox urine supernatant is compared length than ox urine zymotic fluid with the ox urinary precipitation, the 4th day insect population density is continuing decline after the dispenser, and the insect density of urinating zymotic fluid and ox urinary precipitation liquid processed group with ox rose since the 4th day.
Embodiment 5, the compound formulation of this control fruit aphid and two spotted spider mite preparation, it consists of ox urine and urea by raw material, ox is urinated zymotic fluid and water is prepared by 1:3 to 1:7 volume ratio, ox urine zymotic fluid after per 500 milliliters of dilutions adds 50 gram urea, is mixed with the compound formulation that contains 10% urea.
Embodiment 6, the compound formulation of this control fruit aphid and two spotted spider mite preparation, it consists of ox urine and carboxymethyl cellulose by raw material, ox is urinated zymotic fluid and water is prepared by 1:3 to 1:7 volume ratio, ox urine zymotic fluid after per 500 milliliters of dilutions adds 0.05 gram carboxymethyl cellulose, is mixed with the compound formulation that contains 0.01% carboxymethyl cellulose.
Embodiment 7, the compound formulation of this control fruit aphid and two spotted spider mite preparation, it consists of ox urine and by raw material can be good, ox is urinated zymotic fluid and water is prepared by 1:3 to 1:7 volume ratio, ox after per 500 milliliters of dilutions urine zymotic fluid adds 0.5 gram one can be good, be mixed with contain 0.1% 1 can be good compound formulation.
Embodiment 8, the compound formulation of this control fruit aphid and two spotted spider mite preparation, it consists of ox urine and leek extract by raw material, leek smashed to pieces put into gauze bag and directly get juice and obtain the leek extract, ox is urinated zymotic fluid and water is prepared by 1: 3 to 1: 7 volume ratio, ox urine zymotic fluid after per 450 milliliters of dilutions adds 50 milliliters of leek extracts, is mixed with the compound formulation that contains 10% leek extract.
Embodiment 9, the compound formulation of this control fruit aphid and two spotted spider mite preparation, it consists of ox urine and ginger extract by raw material, ginger smashed to pieces put into gauze bag and directly get juice and obtain ginger extract, ox urine and water are prepared by 1: 3 to 1: 7 volume ratio, ox urine after per 450 milliliters of dilutions adds 50 milliliters of ginger extracts, is mixed with the compound formulation that contains 10% ginger extract.
Embodiment 10, the compound formulation of this control fruit aphid and two spotted spider mite preparation, it consists of ox urine and garlic extract by raw material, garlic peeling smashed to pieces put into gauze bag and directly get juice and obtain garlic extract, ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, ox urine after per 450 milliliters of dilutions adds 50 milliliters of garlic extracts, is mixed with the compound formulation that contains 10% garlic extract.
In the foregoing description 5 to 10, the ox urine is ox urine zymotic fluid preferably, and effect is preferable like this.
Be the control efficiency and single comparison with ox urine control efficiency of compound formulation below to Tetranychus cinnabarinus and rose aphid:
With one can be good, six kinds of materials such as carboxymethyl cellulose, urea, leek extract, ginger extract, garlic extract prevent and treat test as the synergist of ox urine to Tetranychus cinnabarinus.Result of the test shows: six kinds of synergist for examination all have significant in various degree synergistic effect to the ox urine zymotic fluid that dilutes for 1:3 to 1:7.Wherein one can be good, the adding of four kinds of synergist such as carboxymethyl cellulose, garlic extract, leek extract compares with ox urine zymotic fluid with single, and the Tetranychus cinnabarinus control efficiency has been improved 23.81%, 20.22%, 18.86% and 17.14% respectively.
The preparation of synergist
Garlic extract: smash to pieces with 500 gram garlics peelings and to put into gauze bag directly to get juice 55ml standby.
Ginger extract: smash to pieces with 500 gram gingers and to put into gauze bag directly to get juice 55ml standby.
Leek extract: smash to pieces with 500 gram leek and to put into gauze bag directly to get juice 70ml standby.
After the ox urine thin up of the two weeks that ferments in the sun is the ox urine of 1:7, accurately measure the ox urine of 3 parts of 450ml dilution 1:3 to 1:7, the leek extract, ginger extract, the garlic extract that wherein add 50ml respectively promptly are made into ox urine, the ox urine that contains 10% leek extract that contains 10% garlic extract, the ox urine that contains 10% ginger extract.
In addition, accurately measure the ox urine of 3 parts of 500ml dilution 1:7 again, wherein adding one of 50 gram urea, 0.5 gram respectively can be good, the carboxymethyl cellulose of 0.05 gram, be made into contain 10% urea, 0.1% one can three kinds of ox urines of carboxymethyl cellulose mixture good, 0.05% standby.Other prepares a ox urine and clear water compares standby.
2.2 test method
2.2.1 the primary election of synergist
Select 24 basin Tetranychus cinnabarinus and rose aphid that serious flowers label respectively takes place in greenhouse, Xinjiang Agricultural Univ forestry institute respectively, and investigate Tetranychus cinnabarinus and the anti-preceding insect population number of rose aphid quantity conduct on it.Above-mentioned 6 kinds of different oxen urine synergist dilutions (urea, carboxymethyl cellulose, one can be good, leek extract, ginger extract, garlic extract) evenly are sprayed on the plant, and each handles 3 repetitions, and establishes contrast with ox urine and clear water.Handling the back is unit statistics Tetranychus cinnabarinus and rose aphid death toll since 1d (d represents the sky) with the blade up to 3d (d represents the sky).
2.2.2 the final election of synergist
According to the control efficiency of just choosing for examination Tetranychus cinnabarinus and rose aphid, select 4 kinds of significant synergist of synergistic effect, 4 kinds of synergist selecting are done same sprinkling by the method among the 2.2.1 handle.
2.2.3 inspection as a result
It is the death toll (dead borer population was added up 1 time every day, up to the 3rd day) of unit inspection statistics Tetranychus cinnabarinus and rose aphid with the blade up to 3d (d represents the sky) since 1d (d represents the sky) that the back is handled in sprinkling.Calculate control efficiency and correcting controling effect.Calculate its control efficiency with following formula:
Formula (1)
P ( % ) = [ 1 - Tn × CKo To × CKn ] × 100
In the formula: P (%) is a control efficiency; T 0Be insect population quantity before preventing and treating; T nFor preventing and treating back insect population quantity; CK 0For handling preceding insect population quantity in the check plot; CK nBe anti-back, check plot insect population quantity.
Correcting controling effect:
Pa ( % ) = P - Po 1 - Po × 100
In the formula: Pa is a correcting controling effect; P is control district control efficiency; P 0Be the check plot control efficiency.
3 results and analysis
3.1 the primary election result and the analysis of synergist
From table 7 and accompanying drawing 7 as seen, 6 kinds of synergist for examination have obvious synergistic effect to the ox urine, especially garlic extract, carboxymethyl cellulose, one can be good, four kinds of synergist such as leek extract, the effect of inhibition Tetranychus cinnabarinus breeding is in various degree all arranged under 1:3 to 1:7 concentration level.Compare with the ox urine with single, the control insecticidal effect has improved 25.48%, 18.99%, 17.97% and 6.9% respectively, and is remarkable to the control efficiency of Tetranychus cinnabarinus.
Data to above 6 kinds of synergist control efficiency are carried out variance analysis, and the result shows F=127.05〉F 0.01=5.06, illustrate that then the control efficiency to Tetranychus cinnabarinus has utmost point significant difference between 6 kinds of medicaments.Especially four kinds of synergist effects are better, that is: carboxymethyl cellulose, can be good, leek extract, garlic extract.
3.2 the primary election result and the analysis of synergist
From table 8 and accompanying drawing 8 as seen, result of the test shows: 6 kinds of synergist for examination have obvious synergistic effect to the ox urine, especially garlic extract, carboxymethyl cellulose, one can be good, four kinds of synergist such as leek extract, inhibition aphid breeding effect was in various degree all arranged under the concentration level at 1: 3 to 1: 7.Compare with the ox urine with single, the control insecticidal effect you can well imagine height 17.18%, 16.5%, 14.7% and 12%.
3.3 the final election result and the analysis of synergist
By table 9 and accompanying drawing 9 as can be known, in 1: 3 to 1: 7 concentration range, the control efficiency of raising ox urine that all can be in various degree wherein one can good synergistic effect highly significant to the ox urine for 4 kinds of synergist of examination.Compare with the ox urine with single, control efficiency has improved 23.81% in final election, and carboxymethyl cellulose, leek extract, garlic extract have improved 20.22%, 18.86% and 17.14% respectively.
Synergistic effect data to above 4 kinds of synergist are carried out variance analysis, and the The results of analysis of variance after the data transaction shows F=329.99〉F 0.01=6.99, then illustrating between 4 kinds of synergist synergistic effects has utmost point significant difference.
The final election result and the analysis of synergist
Select 4 kinds of significant synergist of synergistic effect in 6 kinds of synergist that supply to try according to just choosing, 4 kinds of synergist selecting are done same sprinkling according to the method for just choosing to be handled, the control efficiency of 4 kinds of synergist of computing in 3 days scopes, average control efficiency and correcting controling effect the results are shown in Table 10 and accompanying drawing 10.
From the final election result, for 4 kinds of synergist of examination in 1:3 to 1:7 concentration range, all can be by the control efficiency that improves the ox urine in various degree, wherein one is can good synergistic effect to the ox urine more remarkable.Compare control efficiency with the ox urine and in final election, improved 14.06%, carboxymethyl cellulose in addition, garlic extract, ginger extract have reached 9.97%, 10.57%, 9.77% respectively.
Data to above 4 kinds of synergist control efficiency are carried out variance analysis, and The results of analysis of variance shows F after the data transaction〉F 0.05, illustrate that then the control efficiency to aphid has significant difference between 4 kinds of medicaments.
4 conclusions and discussion
(1) 6 kinds of synergist for examination have obvious synergistic effect to the ox urine, and from the primary election result: carboxymethyl cellulose, can be good, the synergistic effect of garlic extract, leek extract etc. is the most remarkable.Compare with the ox urine with single, carboxymethyl cellulose, one can be good, the Tetranychus cinnabarinus control insecticidal effect of garlic extract, leek extract has improved 18.99%, 17.97%, 25.48% and 6.9% respectively, to control of aphids insecticidal effect aspect: with compare with the ox urine, one can be good, carboxymethyl cellulose, garlic extract, ginger extract have improved 17.18%, 16.5%, 14.7% respectively, 12%, remarkable to the control efficiency of aphid, lasting effect good.
(2) from the final election result, in 1:3 to 1:7 concentration range, raising ox urine that all can be in various degree is to the insecticidal effect of Tetranychus cinnabarinus for 4 kinds of synergist of examination, and wherein one is can good synergistic effect to the ox urine the highest.Compare with the ox urine with single, one can be good control efficiency in final election, improved 23.81%, carboxymethyl cellulose, leek extract, garlic extract have improved 20.22%, 18.86% and 17.14% respectively.But The result of multiple comparisons does not have significant difference between these four kinds of synergist.To aphid insecticidal effect aspect: compare with the ox urine with single, one can be good control efficiency in final election, improved 14.06%, carboxymethyl cellulose, leek extract garlic extract have improved 9.97%, 10.57%, 9.77% respectively.
(3) evidence: the adding of synergist helps improving the control efficiency of ox urine, improves the lethality of insect, once sprays its control efficiency and just can reach more than 90% basically.In the test of being done early stage, the control efficiency of ox urine 1:3 to 1:7 dilution if make reaches more than 90%, at least need spray in 3 days once to connect 3 medicines of spray, just bring up to more than 90% but this test institute supplies 4 kinds of synergist trying that the dilution of ox urine 1:7 is sprayed the 3rd day of medicine for the first time to Tetranychus cinnabarinus and control of aphids effect.This shows, the adding of synergist obviously accelerated the ox urine to Tetranychus cinnabarinus cause death speed, improved control efficiency, reduced the consumption and the spray medicine number of times of ox urine in addition.
Be the test of pesticide effectiveness contrast of ox urine compound formulation and other insecticide below to red date, walnut primary pest:
In the walnut garden respectively to aphid and two spotted spider mite with eight kinds of medicaments: 2000 times of liquid of 48% Le Siben missible oil (being chlopyrifos), 1000 times of liquid of 20% mite gram missible oil, ox urine compound formulation, 2000 times of liquid of 1.8% abamectin emulsifiable concentrate, 1000 times of liquid of 1.2% bitter cigarette emulsion, 5% pyridine worm narrow 2500 times of liquid, 1500 times of liquid of 10% azacyclotin, 1000 times of liquid of 20% dicofol and prevent and treat.The control pilot survey the results are shown in Table 11, table 12.
On August 15th, 2008 is 6 groups, 5 squad jujube tree demonstration gardens in Top Lu Ke township, endanger investigation and control test thereof at the jujube tree two spotted spider mite, selected medicament has: 2000 times of liquid of 48% Le Siben missible oil (being chlopyrifos), 1000 times of liquid of mite gram missible oil, ox urine mixture, 2000 times of liquid of 1.8% abamectin emulsifiable concentrate, 1000 times of liquid of 1.2% bitter cigarette emulsion, 1500 times of liquid of 10% azacyclotin.The control pilot survey the results are shown in Table 13.
In sum, in the present invention, ox urine is that the ox urine can be ox urine supernatant or the ox urinary precipitation liquid of ox urine zymotic fluid or the compound formulation of ox urine that aurochs urine is unfermentable ox urine or ox urine zymotic fluid or ox urine zymotic fluid, the compound formulation of this ox urine consists of ox urine and synergist by raw material, it all has certain effect to control fruit aphid and two spotted spider mite, but effect strengthens in regular turn relatively, can select according to actual needs to use, as: factors such as the quantity of insect, cost.
Table 1 ox urine zymotic fluid (1:3) dilution is to the control efficiency of three kinds of insects
Figure A200810305474D00151
The dilution of table 2 ox urine zymotic fluid variable concentrations is to the control efficiency of rose aphid
Three kinds of ox urines of table 3 are to two spotted spider mite control back insect density variation tendency
Figure A200810305474D00153
Three kinds of ox urines of table 4 are to the control efficiency of two spotted spider mite
Medicament Insect density (head/leaf) before anti- Anti-back insect density (head/leaf) 3d control efficiency (%) Insect density (head/leaf) before anti- Anti-back insect density (head/leaf) 7d control efficiency (%) Total control efficiency (%) Correcting controling effect (%)
Ox urine zymotic fluid 162 101 40.8 90 26.3 94.4 95.9 97.7
Ox urine supernatant 82 42 70.3 19.3 14 94.5 94.5 96.9
Ox urinary precipitation liquid 43 21 68.9 40.3 8 99.6 98.8 99.3
Contrast -33 -41 -63 -67 -88 -77 — —
Table 5 is used medicament aphid insect density variation tendency
Figure A200810305474D00154
Three kinds of ox urines of table 6 are to the anti-pool effect of aphid
Medicament Insect density (head/leaf) before anti- Anti-back insect density (head/leaf) 3d control efficiency (%) Insect density (head/leaf) before anti- Anti-back insect density (head/leaf) 7d control efficiency (%) General mortality rate (%) Corrected mortality (%)
Ox urine zymotic fluid 100 82 48.9 46 13 98.3 98.4 98.6
Ox urine supernatant 194 114 80.7 37 8 99.1 99.0 99.1
Ox bends precipitation and tucks in 109 75 59.5 49 10 98.9 98.8 98.9
Contrast -1 -1 -6 -6 -8 -12 — —
Six kinds of medicaments of table 7 are to the control efficiency of Tetranychus cinnabarinus
Figure A200810305474D00161
Table 8 ox urine synergist is to the control efficiency of aphid
Figure A200810305474D00162
Four kinds of medicaments of table 9 are to the control efficiency of Tetranychus cinnabarinus
Table 10 ox urine synergist is to the preventive effect of aphid
Figure A200810305474D00172
The different medicaments of table 11 are to walnut two spotted spider mite control efficiency
Figure A200810305474D00181
The different medicaments of table 12 are to walnut control of aphids effect
Figure A200810305474D00182
The different medicaments of table 13 are to jujube tree two spotted spider mite control efficiency
Figure A200810305474D00191

Claims (10)

  1. [claim 1] 1, a kind of control fruit aphid and two spotted spider mite preparation is characterized in that this control fruit aphid and two spotted spider mite preparation are the ox urine.
  2. [claim 2] 2, control fruit aphid according to claim 1 and two spotted spider mite preparation, it is characterized in that the ox urine is an ox urine zymotic fluid, this ox urine zymotic fluid obtains as follows: with the filtration of ox urine, the decontamination of gathering, packing in the transparent container, is to ferment 15 to 30 days between 25 to 30 degrees centigrade in temperature.
  3. [claim 3] 3, control fruit aphid according to claim 1 and two spotted spider mite preparation, it is characterized in that the ox urine is the ox urine supernatant of ox urine zymotic fluid, this ox urine supernatant obtains as follows: with the filtration of ox urine, the decontamination of gathering, packing in the transparent container, is to ferment 15 to 30 days between 25 to 30 degrees centigrade in temperature; After fermentation finishes, be under 20 degrees centigrade, left the heart 5 minutes, obtain supernatant and be ox urine supernatant with per minute 6000 in temperature.
  4. [claim 4] 4, control fruit aphid according to claim 1 and two spotted spider mite preparation, it is characterized in that the ox urine is the ox urinary precipitation liquid of ox urine zymotic fluid, this ox urinary precipitation liquid obtains as follows: with the filtration of ox urine, the decontamination of gathering, pack in the transparent container, in temperature is to ferment 15 to 30 days between 25 to 30 degrees centigrade, after fermentation finishes, is under 20 degrees centigrade in temperature, left the heart 5 minutes with per minute 6000, obtaining precipitated liquid is ox urinary precipitation liquid.
  5. [claim 5] 5, a kind of compound formulation according to claim 1 or 2 or 3 or 4 described control fruit aphids and two spotted spider mite preparation is characterized in that consisting of ox urine and synergist by raw material, wherein:
    Synergist is a urea, and ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, and the ox urine after per 500 milliliters of dilutions adds 50 gram urea, is mixed with the compound formulation that contains 10% urea;
    Perhaps, synergist is a carboxymethyl cellulose, and ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, and the ox urine after per 500 milliliters of dilutions adds 0.05 gram carboxymethyl cellulose, is mixed with the compound formulation that contains 0.01% carboxymethyl cellulose;
    Perhaps, synergist is one can be good, and ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, and the ox urine after per 500 milliliters of dilutions adds one of 0.5 gram can be good, be mixed with contain 0.1% 1 can be good compound formulation;
    Perhaps, synergist is the leek extract, leek is smashed to pieces put into gauze bag and directly get juice and obtain the leek extract, and ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, ox urine after per 450 milliliters of dilutions adds 50 milliliters of leek extracts, is mixed with the compound formulation that contains 10% leek extract;
    Perhaps, synergist is a ginger extract, ginger is smashed to pieces put into gauze bag and directly get juice and obtain ginger extract, and ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, ox urine after per 450 milliliters of dilutions adds 50 milliliters of ginger extracts, is mixed with the compound formulation that contains 10% ginger extract;
    Perhaps, synergist is a garlic extract, garlic peeling smashed to pieces put into gauze bag and directly get juice and obtain garlic extract, ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, ox urine after per 450 milliliters of dilutions adds 50 milliliters of garlic extracts, is mixed with the compound formulation that contains 10% garlic extract.
  6. The production method of [claim 6] 6, a kind of control fruit aphid according to claim 2 and two spotted spider mite preparation, it is characterized in that carrying out as follows: with the filtration of ox urine, the decontamination of gathering, packing in the transparent container, is that fermentation obtained ox urine zymotic fluid in 15 to 30 days between 25 to 30 degrees centigrade in temperature.
  7. [claim 7] 7, a kind of production method according to claim 3 or 4 described control fruit aphids and two spotted spider mite preparation, it is characterized in that carrying out as follows: with the filtration of ox urine, the decontamination of gathering, packing in the transparent container, is to ferment 15 to 30 days between 25 to 30 degrees centigrade in temperature; After fermentation finishes, be under 20 degrees centigrade, left the heart 5 minutes, obtain supernatant and be ox urine supernatant, obtain precipitated liquid and be ox urinary precipitation liquid with per minute 6000 in temperature
  8. The production method of [claim 8] 8, a kind of compound formulation according to described control fruit aphid of claim 5 and two spotted spider mite preparation is characterized in that carrying out as follows:
    When synergist is urea, earlier ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, the ox urine after per then 500 milliliters of dilutions adds 50 gram urea, is mixed with the compound formulation that contains 10% urea;
    Perhaps, when synergist is carboxymethyl cellulose, earlier ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, the ox urine after per then 500 milliliters of dilutions adds 0.05 gram carboxymethyl cellulose, is mixed with the compound formulation that contains 0.01% carboxymethyl cellulose;
    Perhaps, synergist is one can be good the time, earlier ox urine and water press the preparation of 1:3 to 1:7 volume ratio, and what the ox urine adding 0.5 after per then 500 milliliters of dilutions restrained one can be good, be mixed with contain 0.1% 1 can be good compound formulation;
    Perhaps, when synergist is the leek extract, earlier leek is smashed to pieces and put into gauze bag and directly get juice and obtain the leek extract, ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, ox urine after per then 450 milliliters of dilutions adds 50 milliliters of leek extracts, is mixed with the compound formulation that contains 10% leek extract;
    Perhaps, when synergist is ginger extract, earlier ginger is smashed to pieces and put into gauze bag and directly get juice and obtain ginger extract, ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, ox urine after per then 450 milliliters of dilutions adds 50 milliliters of ginger extracts, is mixed with the compound formulation that contains 10% ginger extract;
    Perhaps, synergist is a garlic extract, earlier the garlic peeling is smashed to pieces and put into gauze bag and directly get juice and obtain garlic extract, ox urine and water are pressed the preparation of 1:3 to 1:7 volume ratio, ox urine after per then 450 milliliters of dilutions adds 50 milliliters of garlic extracts, is mixed with the compound formulation that contains 10% garlic extract.
  9. [claim 9] 9, a kind of using method according to claim 1 or 2 or 3 or 4 described control fruit aphids and two spotted spider mite preparation, it is characterized in that aurochs urine or ox are urinated zymotic fluid or ox urine supernatant or ox urinary precipitation liquid to fruit tree sprinkling use, using interval is 3 days, be spray in per 3 days once, connect and to spray 3 times.
  10. The using method of [claim 10] 10, a kind of control fruit aphid according to claim 5 and two spotted spider mite preparation is characterized in that compound formulation is sprayed use to fruit tree, and using interval is 3 days, and spray in promptly per 3 days once connects and sprays 3 times.
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CN103880563A (en) * 2014-02-28 2014-06-25 广东省农业科学院果树研究所 Culture medium as well as preparation method and application thereof
CN103891781A (en) * 2013-04-02 2014-07-02 徐兆焕 Efficient pesticide with low toxicity and broad spectrum and preparation method of pesticide
CN103999886A (en) * 2014-05-06 2014-08-27 上海交通大学 Botanical biopesticide for prevention and control of chrysanthemum morifolium aphid and preparation method thereof
CN108698949A (en) * 2015-12-23 2018-10-23 百奥博恩有限公司 Plant quarantine composition

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CN1363219A (en) * 2000-12-31 2002-08-14 张俊山 Efficient biologic germicide and its preparing process
CN1214719C (en) * 2003-09-02 2005-08-17 中国科学院武汉植物研究所 Locust inductive killing agent and its preparing method

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Publication number Priority date Publication date Assignee Title
CN103891781A (en) * 2013-04-02 2014-07-02 徐兆焕 Efficient pesticide with low toxicity and broad spectrum and preparation method of pesticide
CN103880563A (en) * 2014-02-28 2014-06-25 广东省农业科学院果树研究所 Culture medium as well as preparation method and application thereof
CN103880563B (en) * 2014-02-28 2016-01-27 广东省农业科学院果树研究所 A kind of cultivation matrix and preparation method thereof and application
CN103999886A (en) * 2014-05-06 2014-08-27 上海交通大学 Botanical biopesticide for prevention and control of chrysanthemum morifolium aphid and preparation method thereof
CN103999886B (en) * 2014-05-06 2016-09-07 上海交通大学 Botanical biological pesticide of preventing and treating FLOS CHRYSANTHEMI ALBA from Haizhou of China aphid and preparation method thereof
CN108698949A (en) * 2015-12-23 2018-10-23 百奥博恩有限公司 Plant quarantine composition

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