CN101233865A - Matrine agent plant pesticide special for killing aphids and preparation thereof - Google Patents
Matrine agent plant pesticide special for killing aphids and preparation thereof Download PDFInfo
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Abstract
The invention discloses vegetable insecticide of soluble matrine special for killing plant louses and a preparation method thereof, which aims at providing the vegetable insecticide which adopts Chinese herbal medicine as raw material, has high matrine content and low toxin but high efficiency, is harmless to human and livestock as well as special for killing plant louses and the preparation method thereof. The invention comprises 11-13 portions of Chinese herbal medicine extract containing the matrine, 10-20 portions of emulsifier, 4-8 portions of penetrating agent, 0.1 portions of antioxidant and 57-64 portions of deionized water; the Chinese herbal medicine extract containing the matrine is the extract of the mixture by weight of 15-25 portions of sophora flavescens ait, 10-17 portions of chinaberry tree, 3-4 portions of argy wormwood leaf, 6-8 portions of celastrus angulatus maxim, 4-8 portions of derris, 2-4 portions of gardenia, 7-9 portions of herba artemisiae, 10-14 portions of gelsemium elegans, 5-7 portions of orange seed and 7-10 portions of Chinese wingnut leaf. The insecticide of the invention comprises the matrine with super high content about 1.3 percent and is special for killing the plant louses through scientific preparation of a plurality of Chinese herbal medicines and has powerful penetrating performance, high efficiency and insignificant toxin and is in favor of absorption by plants.
Description
Technical field
The invention belongs to pesticide field, in particular, relating to a kind of Chinese herbal medicine that adopts is the matrine agent plant pesticide and preparation method thereof of the special for killing aphids of raw material.
Background technology
China is the maximum country of world population, is again a large agricultural country.Developing rural economy, exploitation agricultural and related industry are one of important policies of China.For a long time, China's vast rural area farm produce production base, because of a large amount of chemical pesticides that use cause the serious three disasters, the pesticide resistance that damage by disease and insect produced has become the latent disease of crops, plant.Residue of pesticide are outstanding day by day to the pollution of food chain and environment.Three disasters problem has not only restricted Agricultural Development, seriously jeopardizes the human life simultaneously with healthy, and the harm that it causes is immeasurable.The long-term harm of using the chemicals agricultural chemicals to environment and related industry displays in people's each side of living.In order to restrict these harm, five kinds of chemical pesticides were destroyed by the Ministry of Agriculture in 2007.The contour malicious organophosphorous pesticide of acephatemet, parathion-methyl, parathion, nuvacron and thiamines will be referred to 288 pesticidal preparations and stops on the crops and use.Therefore, green plant pesticide, bactericide will become main developing direction, and the vast market development prospect is arranged.
Consumption was big when existing vegetable insecticide used on crops, mu cost height, and every mu of ground will use the 250-500 gram, and the labour pays height, and desinsection is not directed special killing.
Summary of the invention
The present invention is in order to overcome weak point of the prior art, and providing a kind of is raw material with the Chinese herbal medicine, to person poultry harmless, low toxicity super-high-efficient, matrine content height, the vegetable insecticide of the special for killing aphids that use cost is low.
Another object of the present invention provides the preparation method of a vegetative insecticide.
The present invention is achieved through the following technical solutions:
A kind of matrine agent plant pesticide of special for killing aphids, it is characterized in that, by weight by Chinese medicine extract 11-13 part of containing matrine, emulsifier 10-20 part, bleeding agent 4-8 part, 0.1 part in antioxidant, deionized water 57-64 part is formed, the described Chinese medicine extract that contains matrine for by weight with kuh-seng 15-25 part, China tree 10-17 part, tarragon 3-4 part, Celastrus angulatus 6-8 part, trifoliate jewelvine 4-8 part, cape jasmine 2-4 part, oriental wormwood 7-9 part, gelsemium elegan 10-14 part, oranges and tangerines seed 5-7 part, it is the extract of raw material that Chinese wingnut leaf 7-10 part is mixed the back, wherein every milliliter of Chinese medicine extract that contains matrine is equivalent to 1.25 gram crude drugs.
Described emulsifier is the 4209-J that Nanjing Teva-Chem. Co., Ltd. produces, and described bleeding agent is high golden thiophene ketone, and described antioxidant is the FF-04 antioxidant that prosperousization great achievement Beijing, Beijing three nine-day periods after the winter solstice chemical industry Co., Ltd produces.
In above-mentioned prescription, can also add dimethyl sulfoxide (DMSO) 1-5 part by weight as disappearing anti-agent.
A kind of preparation method of matrine agent plant pesticide of above-mentioned special for killing aphids is characterized in that, comprises the steps:
(1) takes by weighing kuh-seng, China tree, trifoliate jewelvine, tarragon, Celastrus angulatus, cape jasmine, oriental wormwood, gelsemium elegan, oranges and tangerines seed, Chinese wingnut leaf by weight, in water, soak after 1.5 hours, be heated to 40-50 ℃, kept 20 minutes, add cellulase and carry out enzymolysis, make the abundant stripping of matrine, filter afterwards and obtain herb liquid; Wherein, kuh-seng 15-25 part, China tree 10-17 part, tarragon 3-4 part, Celastrus angulatus 6-8 part, trifoliate jewelvine 4-8 part, cape jasmine 2-4 part, oriental wormwood 7-9 part, gelsemium elegan 10-14 part, oranges and tangerines seed 5-7 part, Chinese wingnut leaf 7-10 part.The consumption of described cellulase can determine that according to conventional production process be preferably 0.3-0.4 part by weight, the unit of activity of cellulase is preferably 100U, and enzymatic hydrolysis condition is that 40 ℃ of constant temperature kept 2.2 hours.
(2) add papain in above-mentioned herb liquid and clarify, the liquid after the clarification leaves standstill and filters the 55-60 ℃ of cryogenic vacuum in back and be concentrated into every milliliter of soup and be equivalent to 1.25 gram crude drugs, obtains containing the Chinese medicine extract of matrine.The consumption of fining agent papain can be determined according to conventional production process.
(3) getting above-mentioned Chinese medicine extract 11-13 part of containing matrine by weight drops in the jar, begin to stir at 20 ℃, and add emulsifier 10-20 part, bleeding agent 4-8 part, 0.1 part in antioxidant, dimethyl sulfoxide (DMSO) 1-5 part successively, deionized water 57-64 part slowly is warming up to 40 ℃ and continues to stir 20 minutes, be cooled to 20 ℃ then, continue stirring again and obtained vegetable insecticide of the present invention in 40 minutes.Described emulsifier is the 4209-J that Nanjing Teva-Chem. Co., Ltd. produces, and described bleeding agent is high golden thiophene ketone, and described antioxidant is the FF-04 antioxidant that prosperousization great achievement Beijing, Beijing three nine-day periods after the winter solstice chemical industry Co., Ltd produces.
The present invention has following technique effect:
Kuh-seng is a pure natural plant, has function clearing heat and detoxicating, desinsection.Contain percentage by weight in the insecticide of the present invention and be about 1.3% superelevation content matrine, science compatibility by various traditional Chinese medicine ingredients, directed special for killing aphids, and, by selecting auxiliary agents such as suitable emulsifier, bleeding agent, antioxidant for use, make the matrine performance mix the efficacy of a drug to greatest extent, permeability is strong, help the absorption of plant, the mu cost is low, and the 23-30 milliliter is only used on every mu of ground, and efficient, low toxicity, the no three disasters, noresidue, pollution-free, no pesticide resistance, not injuring pest natural enemy, is the fully natural green plant pesticide.On crops are used, be suitable for various vegetables, fruit tree, medicinal material, tobacco, tea tree, flowers and other crops, special efficacy arranged killing aphid, nontoxic, harmless to people and animals, have no side effect, can growth promotion, reach the effect of volume increase 10-15%.
Can effectively guarantee the activity of each traditional Chinese medicine ingredients in the insecticide effect of various traditional Chinese medicine ingredients to be not fully exerted by the vegetable insecticide of method of the present invention preparation, reach purpose efficiently.
Embodiment
Below in conjunction with specific embodiment to the detailed description of the invention.
Embodiment 1
To remove impurity through kuh-seng, China tree, trifoliate jewelvine, tarragon, Celastrus angulatus, cape jasmine, oriental wormwood, gelsemium elegan, oranges and tangerines seed, the Chinese wingnut leaf of screening, be ground into meal.Get kuh-seng 25kg, China tree 10kg, trifoliate jewelvine 4Kg, tarragon 4kg, Celastrus angulatus 8kg, cape jasmine 4kg, oriental wormwood 7kg, gelsemium elegan 14kg, oranges and tangerines seed 5kg, Chinese wingnut leaf 7kg put in the extractor, the water logging bubble that adds 4 times of volumes of raw material is after 1.5 hours, be heated to 40-50 ℃, kept 20 minutes.Adding mass percent concentration is that 2% unit of activity is the cellulase 20kg of 100U, and 40 ℃ of constant temperature keep carrying out in 2.2 hours enzymolysis, filter afterwards and obtain herb liquid.Adding mass percent concentration and be 8% papain 6.4kg in above-mentioned herb liquid clarifies, liquid after the clarification leaves standstill and filters back 55-60 degree cryogenic vacuum and be concentrated into every milliliter of soup and be equivalent to 1.25 gram crude drugs, obtains containing the Chinese medicine extract of matrine.
Get the above-mentioned Chinese medicine extract 11kg that contains matrine, be fed in the synthesis tank.Temperature is controlled at 20 ℃ and begins to stir, and add the high golden thiophene ketone 4kg of emulsifier 4209-J 20kg, bleeding agent, antioxidant FF-04 0.1kg, dimethyl sulfoxide (DMSO) 1kg, deionized water 64kg successively, slowly be warming up to 40 ℃, continue to stir 20 minutes, reduce to 20 ℃ then.Continue to stir discharging in 40 minutes packing again and can obtain vegetable insecticide of the present invention.
Embodiment 2
The preparation method is identical with embodiment 1, wherein, kuh-seng 15kg, China tree 17kg, trifoliate jewelvine 8Kg, tarragon 3kg, Celastrus angulatus 6kg, cape jasmine 3kg, oriental wormwood 9kg, gelsemium elegan 12kg, oranges and tangerines seed 7kg, Chinese wingnut leaf 10kg, mass percent concentration is 2% cellulase 20kg, and mass percent concentration is 8% papain 6.4kg.
The Chinese medicine extract 13kg that contains matrine, emulsifier 4209-J 16kg, bleeding agent high golden thiophene ketone 8kg, antioxidant FF-04 0.1kg, deionized water 63kg.
Embodiment 3
The preparation method is identical with embodiment 1, wherein gets kuh-seng 18kg, China tree 15kg, trifoliate jewelvine 4Kg, tarragon 3kg, Celastrus angulatus 7kg, cape jasmine 2kg, oriental wormwood 8kg, gelsemium elegan 10kg, oranges and tangerines seed 6kg, Chinese wingnut leaf 8kg.Mass percent concentration is that 2% unit of activity is the cellulase 20kg of 100U, and mass percent concentration is 8% papain 6.4kg.
The Chinese medicine extract 12kg that contains matrine, emulsifier 4209-J 10kg, the high golden thiophene ketone 8kg of bleeding agent, antioxidant FF-04 0.1kg, dimethyl sulfoxide (DMSO) 5kg, deionized water 64kg.
Embodiment 4
Preparation process is identical with embodiment 1, wherein gets kuh-seng 20kg, China tree 12kg, trifoliate jewelvine 6Kg, tarragon 4kg, Celastrus angulatus 8kg, cape jasmine 4kg, oriental wormwood 8kg, gelsemium elegan 14kg, oranges and tangerines seed 7kg, Chinese wingnut leaf 10kg.Mass percent concentration is that 2% unit of activity is the cellulase 20kg of 100U, and mass percent concentration is 8% papain 6.4kg.The Chinese medicine extract 11kg that contains matrine, emulsifier 4209-J 20kg, the high golden thiophene ketone 7kg of bleeding agent, antioxidant FF-04 0.1kg, dimethyl sulfoxide (DMSO) 5kg, deionized water 57kg.
Embodiment 5
Preparation process is identical with embodiment 1, wherein gets kuh-seng 20kg, China tree 11kg, trifoliate jewelvine 5Kg, tarragon 3kg, Celastrus angulatus 7kg, cape jasmine 3kg, oriental wormwood 7kg, gelsemium elegan 11kg, oranges and tangerines seed 6kg, Chinese wingnut leaf 7kg.Mass percent concentration is that 2% unit of activity is the cellulase 20kg of 100U, and mass percent concentration is 8% papain 6.4kg.Matrine Chinese medicine extract 12kg, emulsifier 4209-J 18kg, the high golden thiophene ketone 5kg of bleeding agent, antioxidant FF-04 0.1kg, dimethyl sulfoxide (DMSO) 2kg, deionized water 63kg.
With vegetable insecticide called after 1.3% aqueous matrine solution of the foregoing description 1 preparation and to carry out the result of field control effectiveness test and toxicity test as follows:
The control cabbage aphid field control effectiveness test report of Inst. of Plant Protection, Jilin Prov. Academy of Agricultural Sciences:
1 test objective
Under various dose, prevent and treat the control efficiency of brassicaceous vegetable cabbage aphid for clear and definite 1.3% aqueous matrine solution (being the vegetable insecticide of the embodiment of the invention 1), determine best field using dosage, concentration, safety and operation technique etc., for the registration of agricultural chemicals, promote scientific basis is provided.
2 experimental conditions
2.1 subjects, crop and breed selection
Aphid comprises black peach aphid (MyZus persicae Sulzer), radish aphid (LipaphiserysimiKalt) and cabbage aphid (Brevicoryne brassicae Linn.)
Wild cabbage (No. 2, iron head)
2.2 environmental condition
This test be selected in Gongzhuling City suburb, Jilin Province to Hua Cunsan society as experimental field, be the main growing vegetables area of Gongzhuling City here, physical features is smooth, is wild cabbage, green onion, Chinese cabbage and other vegetable crops around experimental field.The management of field management and other vegetable fields (weeding and loosen the soil etc. as fertilising, shovel) is consistent.
3 experimental scheme and arrangement
3.1 medicament
3.1.1 test medicine
1.3% aqueous matrine solution (being the vegetable insecticide of the embodiment of the invention 1) (Tianjin Hengyuan Biological Technology Development Co., Ltd.'s production)
3.1.2 contrast medicament
2.5% high-penetrative imidan emulsion (production of Baofeng, Shijiazhuang chemical industry Co., Ltd)
3.1.3 dosing is as shown in table 1 with numbering.
3.2 arrange the sub-district
Sub-district area: 35.0m
2(5 ridges * 0.7m * 10m)
Number of repetition: 4 repetitions, 20 sub-districts are counted in 5 processing.
3.3 application method
3.3.1 using method
Conventional spraying
3.3.2 spraying equipment
Use Singapore's " 16 liters of knapsack sprayers of sharp farming ".
3.3.3 spraying time and number of times
17:30 dispenser on August 23 in 2007 once grows into early stage in heading stage at wild cabbage, and if aphid grows not to be waited the length of time, becomes the aphid occurrence in peak period.
3.3.4 usage amount
Use the conventional sprayer of capacity as 16L, mu is respectively 23.0ml with amount of liquid medicine, 30.8ml, and 38.5ml, the medication multiple is respectively 1700,1300,1000 times. mu water consumption 40L.
3.3.5 prevent and treat the medicament data of other damage by disease and insect
Duration of test and before not used other damage by disease and insect of other chemical control etc.
4 investigation, record and measuring method
4.1 meteorology and soil information
4.1.1 meteorological data
Dispenser same day, cloudy turn to fine for weather, and temperature is 28-15 ℃; Wind-force is the 1-2 level, and wind direction is a southwester.
4.1.2 soil information
This experimental field is positioned at flat area, middle part, Jilin Province, black loam, and pH value 6.5-6.8, the soil is fertile, uses a large amount of farmyard manures, and the humus content height has good granular structure, and soil property is comparatively loose, condition basically identicals such as cultivating and growing and field management.
4.2 investigation method, time and number of times
4.2.1 control time and number of times
1 day, 3 days and investigation respectively in 7 days are investigated 4 times altogether before dispenser, after the dispenser.
4.2.2 investigation method
On the basis of " criterion " investigation method, we are heavy according to aphid generation in this year, density is big, suitable adjustment investigation strain number; Handle 3 ridges, centre on 5 ridges, sub-district during investigation at each, the fixedly 3-4 strain of every ridge, 10 strains are investigated altogether as the reagent target in each sub-district, carry out 4 investigation of dispenser front and back respectively, and a record individual plant worm number.
4.2.3 drug effect computational methods
Insect population rate (%)=(the worm radix of living before the worm radix/dispenser of living after the worm radix-dispenser of living before the dispenser) * 100 of going down
Control efficiency (%)=[1-(CK
0* PT
1)/(CK
1* PT
0)] * 100
PT
0, PT
1An aphid alive number in chemicals treatment district before representing dispenser respectively, after the dispenser
CK
0, CK
1An aphid alive number in blank district before representing dispenser respectively, after the dispenser
4.3 direct influence to crop
Under the dosage of test, to the no poisoning performance of trial crops (wild cabbage).
4.4 to other biotic influence
4.4.1 influence to other damage by disease and insect
Other vegetable-crop pest-insects such as anti-tomato moth, cabbage caterpillar of can holding concurrently.
4.4.2 influence to other non-target organism
Other non-target organism there is not influence substantially.
5 interpretations of result
Suitable dispenser period and consumption:
Suitable dispenser period is that wild cabbage grows into balling early stage (8-10 leaf), and aphid adult and nymph emergence period are dispenser under the situation of peak period and occurrence degree basically identical.The suggestion usage amount is 23.0-30.8ml/ mu (active ingredient 4.5-6.0g/hm
2).
Using method:
Conventional spraying shakes up back and water mixing with reagent agent, then even spraying on cabbage leaves tow sides and cabbage heart, a dispenser, the drug effect phase can reach 7 days.
Safety:
Under test dose to plant growth (wild cabbage) safety.
Statistical form is as shown in table 2 as a result for 1.3% aqueous matrine solution control cabbage aphid field control effectiveness test.Use the Duncan duncan's new multiple range method, it is as shown in table 3 that the borer population remaining alive of 1 day, 3 days and 7 days each treatment regions after the dispenser is carried out statistical analysis, and the control efficiency difference between 1.3% aqueous matrine solution is handled behind (between different dosages) medicine is all not remarkable; With the blank significant difference, reach 0.05 level.
From the statistics of table 2 as can be seen, 1.3% aqueous matrine solution control cabbage aphid effect is fairly obvious.Between each was handled, no matter the dosage height, control efficiency was all above 71.65%, and except that 24h preventive effect 71.65-73.13%, 3 days and 7 days control efficiency all meet or exceed 80%; It is long that the preventive effect of middle and high dosage surpassed for 72.05% drug effect phase, and 7 days insecticidal effects are all above 90% after the dispenser.This pharmacy control efficacy near or surpass the control efficiency of local contrast medicine commonly used.Because this medicament belongs to biologic product, the effect drug effect is slower, and 24h and 72h preventive effect are lower, be lower than the control efficiency of contrast medicine, but after 7 days, control efficiency rises obviously, surpasses contrast medicine preventive effect.
In sum, 1.3% aqueous matrine solution is a kind of effective agent of preventing and treating the full dish cabbage aphid of Cruciferae.Under test dose to plant growth safety.The suggestion usage amount is 4.5-6.0g/hm
2All can (the commodity consumption be a 23.0-30.8ml/ mu).
Tianjin chemicals toxicological detection toxicity test report:
1. rat acute per os toxicity:
According to agricultural chemicals acute oral toxicity grade scale, originally tried the female LD of thing rat acute per os toxicity
50＞5000mg/kgBW, male LD
50＞5000mg/kgBW belongs to low toxicity.
2. rat acute percutaneous toxicity:
According to agricultural chemicals acute dermal toxicity grade scale, originally tried the female LD of thing rat acute percutaneous toxicity
50＞2000mg/kgBW, male LD
50＞2000mg/kgBW belongs to low toxicity.
3. eye stimulates:
According to agricultural chemicals eye irritation grade scale, originally being tried thing eye stimulus intensity is moderate excitant level.
4. acute skin irritation:
According to agricultural chemicals acute skin irritation grade scale, originally being tried thing skin irritatin intensity is slight excitant level.
5. skin allergic reaction:
According to agricultural chemicals skin allergic reaction (sensitization) strength grading standard, originally being tried thing skin allergic reaction (sensitization) intensity is the I level, belongs to weak sensitizer.
Comprehensive above-mentioned result of the test, vegetable insecticide control cabbage aphid effect of the present invention is fairly obvious.The mu cost is low, and the 23-30 milliliter is only used on every mu of ground, and efficient, low toxicity, the no three disasters, noresidue, pollution-free, no pesticide resistance are the fully natural green plant pesticides.
Table 1
| Handle numbering | Medicament | Dispenser dosage (amount of formulation) ml/mu | Formulation rate (active ingredient amount) g/hm 2 |
| 1 | 1.3% aqueous matrine solution | 23.0 | 4.5 |
| 2 | ″ | 30.8 | 6.0 |
| 3 | ″ | 38.5 | 7.5 |
| 4 | 2.5% high-penetrative imidan emulsion (contrast medicament) | 20 | 7.5 |
| 5 | Clear water is handled |
Table 2
| Chemicals treatment | Repeat | Insect population radix (head/10 strains) | Borer population | Preventive effect (%) | Borer population | Preventive effect (%) | Borer population | Preventive effect (%) |
| After the dispenser 1My god | After the dispenser 3My god | After the dispenser 7My god | ||||||
| 23.0ml/mu | 1 | 1579 | 239 | 104 | 35 | |||
| 2 | 1684 | 262 | 117 | 37 | ||||
| 3 | 1694 | 274 | 131 | 43 | ||||
| 4 | 1586 | 260 | 102 | 32 | ||||
| On average | 1635.75 | 258.75 | 71.65 | 113.5 | 78.94 | 36.75 | 90.23 | |
| 30.8ml/mu | 1 | 1704 | 268 | 108 | 36 | |||
| 2 | 1766 | 268 | 114 | 35 | ||||
| 3 | 1754 | 278 | 142 | 51 | ||||
| 4 | 1792 | 280 | 98 | 27 | ||||
| On average | 1754 | 273.5 | 72.05 | 115.50 | 80.01 | 37.25 | 90.76 | |
| 38.5ml/mu | 1 | 1914 | 330 | 115 | 31 | |||
| 2 | 1954 | 305 | 106 | 41 | ||||
| 3 | 1779 | 235 | 130 | 48 | ||||
| 4 | 1865 | 256 | 99 | 25 | ||||
| On average | 1878 | 281.5 | 73.13 | 112.5 | 81.82 | 36.25 | 91.61 | |
| 20ml/mu (contrast medicament) | 1 | 1476 | 180 | 55 | 39 | |||
| 2 | 1411 | 201 | 75 | 45 | ||||
| 3 | 1526 | 195 | 56 | 36 | ||||
| 4 | 1405 | 192 | 53 | 31 | ||||
| On average | 1454.5 | 192 | 76.34 | 59.75 | 87.53 | 37.75 | 88.71 | |
| Blank (clear water processing) | 1 | 1653 | 963 | 583 | 386 | |||
| 2 | 1773 | 948 | 543 | 387 | ||||
| 3 | 1663 | 904 | 553 | 408 | ||||
| 4 | 1670 | 956 | 548 | 373 | ||||
| On average | 1689.75 | 942.75 | 556.75 | 388.5 | ||||
Table 3
| Handle numbering | Behind the medicine 1 day | Behind the medicine 3 days | Behind the medicine 7 days | Behind the medicine _ _ day | ||||
| Preventive effect (%) | The significance of difference | Preventive effect (%) | The significance of difference 0.05 | Preventive effect (%) | The significance of difference 0.05 | Preventive effect (%) | The significance of difference | |
| ck | - | a | a | a | ||||
| 1 | 71.65 | b | 78.94 | b | 90.23 | b | ||
| 2 | 72.05 | b | 80.01 | b | 90.76 | b | ||
| 3 | 73.13 | b | 81.82 | b | 91.61 | b | ||
| 4 | 76.34 | b | 87.53 | b | 88.71 | b | ||
Claims (6)
1, a kind of matrine agent plant pesticide of special for killing aphids, it is characterized in that, comprise Chinese medicine extract 11-13 part of containing matrine by weight, emulsifier 10-20 part, bleeding agent 4-8 part, 0.1 part in antioxidant, deionized water 57-64 part, the described Chinese medicine extract that contains matrine for by weight with kuh-seng 15-25 part, China tree 10-17 part, tarragon 3-4 part, Celastrus angulatus 6-8 part, trifoliate jewelvine 4-8 part, cape jasmine 2-4 part, oriental wormwood 7-9 part, gelsemium elegan 10-14 part, oranges and tangerines seed 5-7 part, it is the extract of raw material that Chinese wingnut leaf 7-10 part is mixed the back, wherein every milliliter of Chinese medicine extract that contains matrine is equivalent to 1.25 gram crude drugs.
2, the matrine agent plant pesticide of special for killing aphids according to claim 1 is characterized in that, described emulsifier is 4209-J, and described bleeding agent is high golden thiophene ketone, and described antioxidant is FF-04.
3, the matrine agent plant pesticide of special for killing aphids according to claim 1 and 2 is characterized in that, also contains dimethyl sulfoxide (DMSO) 1-5 part by weight.
4, the preparation method of the matrine agent plant pesticide of the described special for killing aphids of a kind of claim 1 is characterized in that, comprises the steps:
(1) takes by weighing kuh-seng, China tree, trifoliate jewelvine, tarragon, Celastrus angulatus, cape jasmine, oriental wormwood, gelsemium elegan, oranges and tangerines seed, Chinese wingnut leaf by weight, in water, soak after 1.5 hours, be heated to 40-50 ℃, kept 20 minutes, add cellulase and carry out enzymolysis, filter and obtain herb liquid; Wherein, kuh-seng 15-25 part, China tree 10-17 part, tarragon 3-4 part, Celastrus angulatus 6-8 part, trifoliate jewelvine 4-8 part, cape jasmine 2-4 part, oriental wormwood 7-9 part, gelsemium elegan 10-14 part, oranges and tangerines seed 5-7 part, Chinese wingnut leaf 7-10 part;
(2) add papain in above-mentioned herb liquid and clarify, the liquid after the clarification leaves standstill and filters the back cryogenic vacuum and be concentrated into every milliliter of soup and be equivalent to 1.25 gram crude drugs, obtains containing the Chinese medicine extract of matrine;
(3) getting above-mentioned Chinese medicine extract 11-13 part of containing matrine by weight drops in the jar, begin to stir at 20 ℃, and add emulsifier 10-20 part, bleeding agent 4-8 part, 0.1 part in antioxidant, dimethyl sulfoxide (DMSO) 1-5 part successively, deionized water 57-64 part slowly is warming up to 40 ℃ and continues to stir 20 minutes, be cooled to 20 ℃ then, continue stirring again and obtained vegetable insecticide of the present invention in 40 minutes.
5, the preparation method of vegetable insecticide according to claim 4 is characterized in that, the parts by weight of described cellulase are 0.3-0.4 part, and the unit of activity of cellulase is 100U, and enzymatic hydrolysis condition is that 40 ℃ of constant temperature kept 2.2 hours.
6, the preparation method of vegetable insecticide according to claim 4 is characterized in that, described emulsifier is 4209-J, and described bleeding agent is high golden thiophene ketone, and described antioxidant is FF-04.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204577A (en) * | 2011-03-28 | 2011-10-05 | 山东大学威海分校 | Compound ailanthus preparation and applications thereof |
| CN102972451A (en) * | 2012-12-10 | 2013-03-20 | 大连创达技术交易市场有限公司 | Novel alkaline water |
| CN103222469A (en) * | 2013-05-16 | 2013-07-31 | 王月顺 | Method for producing sophocarpidine water aqua by using enzymolysis method |
| CN104365687A (en) * | 2014-11-26 | 2015-02-25 | 曾少兰 | Sophocarpidine insecticide aqueous agent and preparation method thereof |
| CN104446855A (en) * | 2013-09-23 | 2015-03-25 | 丹阳恒安化学科技研究所有限公司 | Production technology for efficient environment-friendly pesticide |
| CN106342844A (en) * | 2016-08-31 | 2017-01-25 | 周翠华 | Organic and no-residue pesticide sprayed by unmanned aerial vehicle |
| CN109776214A (en) * | 2018-12-25 | 2019-05-21 | 金华市野泉园艺技术有限公司 | A kind of composition of plant disease control |
-
2008
- 2008-02-26 CN CNA2008100523244A patent/CN101233865A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204577A (en) * | 2011-03-28 | 2011-10-05 | 山东大学威海分校 | Compound ailanthus preparation and applications thereof |
| CN102204577B (en) * | 2011-03-28 | 2013-05-29 | 山东大学威海分校 | Compound ailanthus preparation and applications thereof |
| CN102972451A (en) * | 2012-12-10 | 2013-03-20 | 大连创达技术交易市场有限公司 | Novel alkaline water |
| CN103222469A (en) * | 2013-05-16 | 2013-07-31 | 王月顺 | Method for producing sophocarpidine water aqua by using enzymolysis method |
| CN103222469B (en) * | 2013-05-16 | 2014-07-02 | 山西安顺生物科技有限公司 | Method for producing sophocarpidine water aqua by using enzymolysis method |
| CN104446855A (en) * | 2013-09-23 | 2015-03-25 | 丹阳恒安化学科技研究所有限公司 | Production technology for efficient environment-friendly pesticide |
| CN104365687A (en) * | 2014-11-26 | 2015-02-25 | 曾少兰 | Sophocarpidine insecticide aqueous agent and preparation method thereof |
| CN106342844A (en) * | 2016-08-31 | 2017-01-25 | 周翠华 | Organic and no-residue pesticide sprayed by unmanned aerial vehicle |
| CN109776214A (en) * | 2018-12-25 | 2019-05-21 | 金华市野泉园艺技术有限公司 | A kind of composition of plant disease control |
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