CN101491273A - Grape postharvest diseases or fruit abscission prevention and control method and special prevention and control agent - Google Patents
Grape postharvest diseases or fruit abscission prevention and control method and special prevention and control agent Download PDFInfo
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Abstract
The invention discloses a method for preventing and controlling postharvest disease or berry falling of a grape fruit and a special preventing agent thereof. The method comprises a process for treating a picked grape by the substances: 1) borate; or 2) the borate and a biological bactericide. The use concentration of the borate can be between 0.1 and 1 percent; and the use concentration of the biological bactericide can be a bacterial liquid of 1*10<4>-1*10<9>CFU Cryptococcus laurentii/ml. The invention also discloses the preventing agent for postharvest disease or berry falling of the grape fruit, wherein active ingredients of the preventing agent comprise the borate, and further the biological bactericide. The preventing method and the special preventing agent thereof not only can prevent and control the postharvest disease and the berry falling of the grape fruit, but also can reduce dosage of chemical pesticide and toxic residue on fruit and vegetable products.
Description
Technical field
The present invention relates to the control of crops postharvest disease or fruit abscission, be specifically related to the method for preventing and treating and the control agent of postharvest disease of fruits and vegetables or fruit abscission.
Background technology
Grape is one of fruit of liking of people.Adopt that rot in the back and fruit abscission is a modal problem in the grape fruit preservation and freshness, and this two aspect exists close ties.On the one hand, pathogen infection is one of reason that causes fruit abscission.On the other hand, after the fruit abscission, because the generation of wound can be quickened pathogen infecting fruit.Therefore, coming off and the generation of disease of control grape fruit interknits, and complements each other.To effective control of grape disease, with the control that greatly promotes fruit abscission.The conventional method of control fruit disease and shattering is to use chemical pesticide and hormone respectively.Yet, use chemical agent to cause many pathogens to produce the resistance to the action of a drug for a long time, make the protection effect of chemical pesticide reduce, working concentration increases year by year.Simultaneously, the use agricultural chemicals of high concentration contaminated environment not only in the production, the residual hazard of fruit surface exceeds standard and is also threatening human health.And for hormone, long-term use can bring many drawbacks, influences the normal mature and the quality of fruit.
Corresponding, as the alternative measure of chemical pesticide, biological bactericide becomes the focus of countries in the world research owing to characteristics such as safe, efficient, easy-to-use.Yet, though biological bactericide has been showed good prospects for application in the control fruit postharvest diseases, but because bacteria agent is based on viable bacteria, be different from chemical bactericide, it presses down sick effect and is subjected to all multifactor influences, its limitation and ageing is arranged, use the antagonism bacterium that the prevention effect of disease is difficult to compare favourably with chemical agent separately.Therefore, need improve the biological and ecological methods to prevent plant disease, pests, and erosion effectiveness of antagonism bacterium by the whole bag of tricks and means, this has become the emphasis and the hot issue of current biological control research field.
Boron be between metal and nonmetal between a kind of element, can become various borides with non-metallic symphysis again with metal.Boron and boride have wide application prospect, are widely used in fields such as industry, agricultural, national defence, the most advanced branches of science, medical science.In fields such as agriculturals, boron is the indispensable micronutrient element of plant normal growth.It is present in organic substance, various soil mineral (as tourmaline) and is adsorbed in the soil particle soil liquid surperficial and balance with it.The result who uses boron fertilizer both at home and abroad shows that rape, wheat, cotton, fruit tree etc. are had tangible effect of increasing production, the soil of serious boron deficiency, and using back output can be doubled and redoubled.Studies show that the low concentration borate solution does not produce harm to human body, can use safely.
Summary of the invention
The object of the present invention is to provide the method for preventing and treating of a kind of grape postharvest diseases or fruit abscission.
The method of preventing and treating of grape postharvest diseases provided by the present invention or fruit abscission comprises the grape after plucking with following 1) or 2) the process handled of material:
1) borate;
2) borate and biological bactericide.
Described borate is the solubility borate, is preferably Boratex or potassium borate.
Described boratory working concentration can be 0.1～1g/100ml.
In actual use, can be with the grape after plucking with described borate aqueous solution soaking 3-20 minute, as immersion 3-5 minute, or 10-20 minute.
Described biological bactericide can be the biological bactericide of control postharvest disease of fruits and vegetables, as the biological bactericide of control grape postharvest disease.The active component of the biological bactericide of described grape postharvest disease can be Cryptococcus laurentii (Cryptococcus laurentii).The working concentration of this biological bactericide can be 1 * 10
4-1 * 10
9CFU Cryptococcus laurentii (Cryptococcus laurentii)/ml bacterium liquid.Prevent and treat in the process, borate and biological bactericide compounding application in one embodiment, can be soaked the control agent that mixes that the grape after plucking is formed with the described borate aqueous solution and Cryptococcus laurentii (Cryptococcus laurentii); In another embodiment, the grape after plucking can be soaked with described borate solution earlier, use described Cryptococcus laurentii (Cryptococcus laurentii)/ml bacterium liquid to soak afterwards again.
Another object of the present invention is to provide the control agent of a kind of grape postharvest diseases or fruit abscission.
The control agent of grape postharvest diseases provided by the present invention or fruit abscission, its active component comprises borate, is preferably the solubility borate, further can be Boratex or potassium borate.
Further, described active component also can comprise described biological bactericide.
Described control agent also can comprise other assistant agent commonly used.
Described biological bactericide can be the bacteria agent of control postharvest disease of fruits and vegetables, as the biological bactericide of control grape postharvest disease.The active component of the biological bactericide of described grape postharvest disease can be Cryptococcus laurentii (Cryptococcus laurentii).
But borate in the described control agent and described biological bactericide independent packaging also can mix.
When the borate in the described control agent and described biological bactericide mixed, the proportioning of described borate and Cryptococcus laurentii (Cryptococcus laurentii) was 2.62 * 10
-4-2.62 * 10
-3Mol borate ion: 1 * 10
6-1 * 10
11CFU Cryptococcus laurentii (Cryptococcus laurentii).
Certainly, the active component of described control agent specifically can be described borate and described biological bactericide.
Experiment showed, that the method for preventing and treating of the present invention can be used for preventing and treating grape postharvest diseases with the control agent, as gray mold, anthracnose and white rot.
The present invention provides the method for preventing and treating and the control agent of grape postharvest diseases or fruit abscission according to the new purposes of borate in control grape postharvest diseases or fruit abscission.
Control agent of the present invention is easy to use, both can also can use by the method that sprays by soaking, and effect is remarkable, and especially active component comprises the control agent of borate and biological bactericide.Experiment shows: the grape that 1% the Boratex aqueous solution is handled is separately compared with undressed grape, and the total incidence of its gray mold, anthracnose and white rot has dropped to 37.8% from 83.9%; Borate cooperates the grape of handling to compare with undressed grape with biological bactericide, and the total incidence of its gray mold, anthracnose and white rot has dropped to 9.8% from 81.8%.
Use control agent of the present invention to prevent and treat postharvest disease of fruits and vegetables and fruit abscission, can reduce the consumption of chemical pesticide and the residual hazard on fruit and vegetable food, to protecting health and preventing the pollution of the environment all very important.Simultaneously, also help the foreign exchange earning of China's high-quality fruit and vegetable food, economic benefit and social benefit very are remarkable.
Description of drawings
Fig. 1 handles the prevention effect figure that grape fruit is adopted back gray mold and anthracnose for Boratex under the room temperature;
Fig. 2 is that Boratex is handled the prevention effect figure that grape fruit is adopted back gray mold and anthracnose under the low temperature (0 ℃);
Fig. 3 adopts the prevention effect figure of back gray mold and anthracnose to grape fruit for Boratex under the room temperature and the processing of antagonism bacterium;
Fig. 4 is that Boratex and antagonism bacterium are handled the prevention effect figure that grape fruit is adopted back gray mold and anthracnose under the low temperature (0 ℃);
Fig. 5 adopts the prevention effect figure of back fruit abscission to grape fruit for Boratex under the room temperature and the processing of antagonism bacterium;
Fig. 6 is that Boratex and antagonism bacterium are handled the prevention effect figure that grape fruit is adopted the back fruit abscission under the low temperature (0 ℃);
Fig. 7 handles the prevention effect figure that grape fruit is adopted back gray mold and anthracnose for potassium borate under the room temperature;
Fig. 8 handles for Boratex under the room temperature wood is received the prevention effect figure of gray mold and anthracnose after the lattice grape harvest.
The specific embodiment
Percentage composition among the following embodiment if no special instructions, is the quality percentage composition.
Control treatment among the present invention is exactly only to add H
2O, and do not add the processing that other prevents and treats agent.
The fruit incidence of disease=refer to that the fruit sum of the fruit number/investigation of the visible scab of naked eyes appears in the surface.
Embodiment 1: Boratex is handled the control of grape fruit being adopted back gray mold and anthracnose under the room temperature.
1.1 experiment material
Fruit: (Vitis vinifera L.cv.Wuhebai) is test material with Xinjiang thompson seedless grape fruit.Fruit derives from Xinjiang.Precooling under 0 ℃ of condition immediately behind the fruit harvesting, and transport the Institute of Botany, Chinese Academy of Sciences laboratory to refrigerator car.
Pathogen: ash arrhizus bacteria (Botrytis cinerea) and anthrax bacteria (Colletotrichumgloeosporioides), directly separate from the morbidity fruit surface.Working concentration all is 1 * 10
4CFU/ml.
1.2 processing method
1) grape fruit is 1 * 10 in concentration
4Immersion treatment is 10 minutes in the ash arrhizus bacteria solution of CFU/ml, treats to soak 15 minutes with following control agent respectively after fruit surface dries: A, 0.1% the Boratex aqueous solution; B, 0.5% the Boratex aqueous solution; C, 1% the Boratex aqueous solution; D, H
2O.
2) grape fruit is 1 * 10 in concentration
4Immersion treatment 10 minutes respectively in the anthrax bacteria solution of CFU/ml, treat fruit surface dry after respectively with following control agent immersion 15 minutes: A, 0.1% the Boratex aqueous solution; B, 0.5% the Boratex aqueous solution; C, 1% the Boratex aqueous solution; D, H
2O.
After grape fruit handled according to above-mentioned two kinds of methods, preserve under room temperature, observe its incidence after 3 days, 3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
1.3 experimental result:
Gray mold and anthracnose had significant inhibition effect after Boratex was adopted grape fruit under the normal temperature, referring to Fig. 1, showed the raising along with the Boratex working concentration, and its prevention effect also significantly improves.
Embodiment 2. low temperature (0 ℃) Boratex are down handled the control of grape fruit being adopted back gray mold and anthracnose.
2.1 experiment material
Fruit: (Vitis vinifera L.cv.Wuhebai) is test material with Xinjiang thompson seedless grape fruit.Fruit derives from Xinjiang.Precooling under 0 ℃ of condition immediately behind the fruit harvesting, and transport the Institute of Botany, Chinese Academy of Sciences laboratory to refrigerator car.
Pathogen: ash arrhizus bacteria (Botrytis cinerea) and anthrax bacteria (Colletotrichumgloeosporioides), directly separate from the morbidity fruit surface.Working concentration all is 1 * 10
4CFU/ml.
2.2 processing method
After the two kind methods processing of grape fruit,, observe its incidence after 3 days in 0 ℃ of storage down according to embodiment 1.3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
2.3 experimental result:
Gray mold and anthracnose had significant inhibition effect after Boratex was adopted grape fruit under the low temperature (0 ℃), and referring to Fig. 2, along with the raising of Boratex working concentration, its prevention effect also improves.
Above-mentioned experiment confirm the prevention effect of Boratex to grape postharvest diseases.
The embodiment 3:1% Boratex aqueous solution is used to prevent and treat the grape postharvest disease
Take by weighing 100 gram Boratexes, it is stand-by to be mixed with 1% the aqueous solution.3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
Get 2 kilograms of Xinjiang thompson seedless grape fruits (Vitis vinifera L.cv.Wuhebai), wherein half soaked 3～5 minutes in the 1% Boratex aqueous solution, taking out the back dries naturally, be placed in the carton, second half does not do any processing, be placed in the same carton and preserve under low temperature (0 ℃), keep 95% relative humidity; Observe the incidence of disease of gray mold, anthracnose and white rot after 60 days.The result is as shown in table 1:
After table 1 Boratex was handled, grape fruit was adopted the incidence of disease (0 ℃) of the main disease in back
| Handle | Gray mold (%) | Anthracnose (%) | White rot (%) | Total incidence (%) |
| Contrast | 35.2±1.4 | 23.1±1.1 | 25.6±1.5 | 83.9±3.4 |
| Boratex (1%) | 15.1±0.9 | 9.2±0.2 | 13.5±0.7 | 37.8±2.3 |
By data in the table as can be seen, after depositing through 60 days, the sample total incidence of handling through Boratex is about 38%, and undressed sample total incidence reaches 83.9%, illustrates that this routine processing method and control agent have than positive effect the control grape postharvest diseases.
The sample that this example is handled through Boratex, with the clear water cleaning, after normally washing by rubbing with the hands and soaking 1 minute, the Boratex that remains on the fruit crust can be removed, and does not influence edible before edible.
Embodiment 4: Boratex and biological bactericide are handled the prevention effect of grape fruit being adopted back gray mold and anthracnose under the room temperature.
4.1 experiment material
Fruit: (Vitis vinifera L.cv.Wuhebai) is test material with Xinjiang thompson seedless grape fruit.Fruit derives from Xinjiang.Precooling under 0 ℃ of condition immediately behind the fruit harvesting, and transport the Institute of Botany, Chinese Academy of Sciences laboratory to refrigerator car.
Pathogen: ash arrhizus bacteria (Botrytis cinerea) and anthrax bacteria (Colletotrichumgloeosporioides), directly the fruit surface from morbidity separates.Working concentration all is 1 * 10
4CFU/ml.
Antagonism bacterium: Cryptococcus laurentii (Cryptococcus laurentii (Kuffer.) Skinner) BAY-3CGMCC № 1029, this bacterial strain separates from the apple fruit surface, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (being called for short CGMCC) on November 12nd, 2003, preserving number is CGMCC № 1029.
4.2 processing method
1) grape fruit is 1 * 10 in concentration
4Immersion treatment is 10 minutes in the ash arrhizus bacteria solution of CFU/ml, treats to soak 15 minutes with following control agent respectively after fruit surface dries: A, contrast; B, 0.5% the Boratex aqueous solution; C, 1 * 10
6The Cryptococcus laurentii of CFU/ml (Cryptococcus laurentii) aqueous solution; D, solute are 0.5% (final concentration) Boratex and 1 * 10
6The aqueous solution of CFU/ml (final concentration) Cryptococcus laurentii Cryptococcus laurentii, its ratio is 1.31 * 10
-3Mol Boratex: 1 * 10
8CFU Cryptococcus laurentii (Cryptococcuslaurentii).
2) grape fruit is 1 * 10 in concentration
4The difference immersion treatment is 10 minutes in the anthrax bacteria solution of CFU/ml, treats to soak 15 minutes with following control agent respectively after fruit surface dries: A, contrast; B, 0.5% the Boratex aqueous solution;
C, 1 * 10
6The Cryptococcus laurentii of CFU/ml (Cryptococcus laurentii) aqueous solution; D, solute are 0.5% (final concentration) Boratex and 1 * 10
6The aqueous solution of CFU/ml (final concentration) Cryptococcus laurentii, its ratio is 1.31 * 10
-3Mol Boratex: 1 * 10
8CFU Cryptococcus laurentii (Cryptococcuslaurentii).
Grape fruit respectively according to after above-mentioned two kinds of methods processing, is preserved under room temperature, observed its incidence after 3 days.3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
4.3 experimental result:
Gray mold and anthracnose all had significant inhibition effect (Fig. 3) after the independent use of Boratex or biological bactericide was adopted grape fruit under the normal temperature, and it is better that both are used effect.
Embodiment 5: Boratex and biological bactericide are handled the prevention effect of grape fruit being adopted back gray mold and anthracnose under the low temperature (0 ℃).
5.1 experiment material
Fruit: (Vitis vinifera L.cv.Wuhebai) is test material with Xinjiang thompson seedless grape fruit.Fruit derives from Xinjiang.Precooling under 0 ℃ of condition immediately behind the fruit harvesting, and transport Institute of Botany, Chinese Academy of Sciences to refrigerator car.
Pathogen: ash arrhizus bacteria (Botrytis cinerea) and anthrax bacteria (Colletotrichumgloeosporioides).Working concentration all is 1 * 10
4CFU/ml.
Antagonism bacterium: Cryptococcus laurentii (Cryptococcus laurentii)
5.2 processing method
1) grape fruit is 1 * 10 in concentration
4Immersion treatment is 10 minutes in the ash arrhizus bacteria solution of CFU/ml, treats to soak 15 minutes with following control agent respectively after fruit surface dries: A, contrast; B, 0.5% the Boratex aqueous solution; C, 1 * 10
9The Cryptococcus laurentii of CFU/ml (Cryptococcus laurentii) aqueous solution; D, solute are 0.1% (final concentration) Boratex and 1 * 10
9The aqueous solution of CFU/ml (final concentration) Cryptococcus laurentii, its ratio is 2.62 * 10
-4Mol Boratex: 1 * 10
11CFU Cryptococcus laurentii (Cryptococcus laurentii).
2) grape fruit is 1 * 10 in concentration
4The difference immersion treatment is 10 minutes in the anthrax bacteria solution of CFU/ml, treats to soak 15 minutes with following control agent respectively after fruit surface dries: A, contrast; B, 0.1% the Boratex aqueous solution; C, 1 * 10
9The Cryptococcus laurentii of CFU/ml (Cryptococcus laurentii) aqueous solution; D, solute are 0.5% (final concentration) Boratex and 1 * 10
9The aqueous solution of CFU/ml (final concentration) Cryptococcuslaurentii, its ratio is 2.62 * 10
-4Mol Boratex: 1 * 10
11CFU Cryptococcus laurentii (Cryptococcus laurentii).
After grape fruit handled according to above-mentioned two kinds of methods, preserve under low temperature (0 ℃), observe its incidence after 3 days.3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
5.3 experimental result:
Gray mold and anthracnose all had significant inhibition effect (Fig. 4) after the independent use of Boratex or biological bactericide was adopted grape fruit under the low temperature (0 ℃), and it is better that both are used effect.
The embodiment 6:1% Boratex aqueous solution and 1 * 10
6CFU/ml biological bactericide control grape postharvest diseases
Take by weighing 100 gram Boratexes, it is stand-by to be mixed with 1% the aqueous solution, and preparing concentration in addition is 1 * 10
6The Cryptococcus laurentii of CFU/ml (Cryptococcus laurentii) water slurry is as biological bactericide, and 3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
Get 2 kilograms of Xinjiang thompson seedless grape fruits (Vitis vinifera L.cv.Wuhebai), wherein half soaked 5 minutes in the Boratex aqueous solution, taking out the back dries naturally, in Cryptococcus laurentii (Cryptococcuslaurentii) water slurry, soaked 2 minutes again, be placed in the carton then; Second half does not do any processing, is placed in the same carton and preserves under low temperature (0 ℃), keeps 95% relative humidity; Observe its incidence of disease after 60 days.Record the results are shown in Table 3:
Table 3 Boratex and biological bactericide are to adopting the incidence of disease (0 ℃) of the main disease after the back grape fruit is handled
| Handle | Gray mold (%) | Anthracnose (%) | White rot (%) | Total incidence (%) |
| Contrast | 37.5±2.1 | 25.5±1.1 | 18.8±1.5 | 81.8±4.5 |
| Boratex+biological bactericide | 6.6±0.4 | 3.2±0.1 | 0±0 | 9.8±0.3 |
By table 3 data as can be seen, after depositing through 60 days, the treated sample incidence of disease is controlled in 10%, and undressed sample total incidence reaches more than 80%, illustrates that this routine processing method and control agent can obviously suppress the generation of grape postharvest diseases.
Boratex and biological bactericide are handled the prevention effect of grape fruit being adopted the back fruit abscission under embodiment 7. room temperatures.
7.1 experiment material
Fruit: (Vitis vinifera L.cv.Wuhebai) is test material with Xinjiang thompson seedless grape fruit.Fruit derives from Xinjiang.Precooling under 0 ℃ of condition immediately behind the fruit harvesting, and transport the Institute of Botany, Chinese Academy of Sciences laboratory to refrigerator car.
7.2 processing method
Grape fruit soaked 15 minutes in following control agent: A, contrast; B, 0.5% the Boratex aqueous solution; C, 1 * 10
4The Cryptococcus laurentii of CFU/ml (Cryptococcus laurentii) aqueous solution; D, solute are 0.5% (final concentration) Boratex and 1 * 10
4The aqueous solution of CFU/ml (final concentration) Cryptococcus laurentii, its ratio is 1.31 * 10
-3Mol Boratex: 1 * 10
6CFU Cryptococcus laurentii (Cryptococcus laurentii).Treat to preserve under room temperature after fruit surface dries.Observe the grape fruit dropping situations after 7 days.Embody the fruit abscission situation with fruit drop rate (fruit grain number that comes off/total fruit grain number to be measured).3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
7.3 experimental result:
The independent use of Boratex or biological bactericide is adopted the back fruit abscission to grape fruit under the normal temperature all certain prevention effect (Fig. 5), and it is better that both are used effect.
Boratex and biological bactericide are handled the prevention effect of grape fruit being adopted the back fruit abscission under embodiment 8. low temperature (0 ℃).
8.1 experiment material
Fruit: (Vitis vinifera L.cv.Wuhebai) is test material with Xinjiang thompson seedless grape fruit.Fruit derives from Xinjiang.Precooling under 0 ℃ of condition immediately behind the fruit harvesting, and transport the Institute of Botany, Chinese Academy of Sciences laboratory to refrigerator car.
8.2 processing method
Grape fruit soaked 15 minutes in following control agent: A, contrast; B, 1% the Boratex aqueous solution; C, 1 * 10
9The Cryptococcus laurentii of CFU/ml (Cryptococcus laurentii) aqueous solution; D, solute are 0.5% (final concentration) Boratex and 1 * 10
6The aqueous solution of CFU/ml (final concentration) Cryptococcus laurentii, its ratio is 1.31 * 10
-3Mol Boratex: 1 * 10
9CFU Cryptococcus laurentii (Cryptococcus laurentii).Treat to preserve under low temperature after fruit surface dries.Observe the grape fruit dropping situations after 30 days.3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
8.3 experimental result:
The independent use of Boratex or biological bactericide is adopted the back fruit abscission to grape fruit under the low temperature (0 ℃) all certain prevention effect (Fig. 6), and it is better that both are used effect.
Embodiment 9. potassium borates are adopted the prevention effect of back gray mold and anthracnose to grape fruit.
9.1 experiment material
Fruit: (Vitis vinifera L.cv.Wuhebai) is test material with Xinjiang thompson seedless grape fruit.Fruit derives from Xinjiang.Precooling under 0 ℃ of condition immediately behind the fruit harvesting, and transport the Institute of Botany, Chinese Academy of Sciences laboratory to refrigerator car.
Pathogen: ash arrhizus bacteria (Botrytis cinerea) and anthrax bacteria (Colletotrichum gloeosporioides).Working concentration all is 1 * 10
4The CFU/ml bacteria suspension.
9.2 processing method
Grape fruit is 1 * 10 in concentration
4Immersion treatment is 10 minutes in the ash arrhizus bacteria of CFU/ml and the anthrax bacteria solution, treats to soak 15 minutes with following control agent respectively after fruit surface dries: A, 0.1% the potassium borate aqueous solution; B, 0.5% the potassium borate aqueous solution; C, 1% the potassium borate aqueous solution; D, H
2O.Fruit is preserved under room temperature after handling.Observe its incidence after 3 days.3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
9.3 experimental result:
Gray mold and anthracnose had significant inhibition effect referring to Fig. 7 after potassium borate was adopted grape fruit under the normal temperature, and along with the raising of potassium borate working concentration, its prevention effect also significantly improves.
10.1 experiment material
Fruit: with Xinjiang Mu Na lattice grape is test material.Fruit derives from Xinjiang.Precooling under 0 ℃ of condition immediately behind the fruit harvesting, and transport the Institute of Botany, Chinese Academy of Sciences laboratory to refrigerator car.
Pathogen: ash arrhizus bacteria (Botrytis cinerea) and anthrax bacteria (Colletotrichum gloeosporioides).Working concentration all is 1 * 10
4CFU/ml.
10.2 processing method
It is 1 * 10 in concentration that wood is received the lattice grape
4Immersion treatment is 10 minutes in the ash arrhizus bacteria of CFU/ml and the anthrax bacteria solution, treats to soak 15 minutes with following control agent respectively after fruit surface dries: A, 0.1% Boratex; B, 0.5% Boratex; C, 1% Boratex; D, H
2O.Fruit is preserved under room temperature after handling.Observe its incidence after 3 days.3 repetitions are established in experiment, and each handles each replication 2.5kg grape.
10.3 experimental result:
Boratex receives to wood that gray mold and anthracnose have significant inhibition effect referring to Fig. 8 after the lattice grape harvest under the normal temperature, and along with the raising of Boratex working concentration, its prevention effect also significantly improves.
Claims (10)
1, the method for preventing and treating of grape postharvest diseases or fruit abscission is characterized in that, described method comprises the grape after plucking with following 1) or 2) the process handled of material:
1) borate;
2) borate and biological bactericide.
2, the method for preventing and treating according to claim 1 is characterized in that, described borate is the solubility borate, is preferably Boratex or potassium borate.
3, the method for preventing and treating according to claim 2 is characterized in that, described boratory working concentration is 0.1-1g/100ml.
According to arbitrary described method of preventing and treating among the claim 1-3, it is characterized in that 4, described biological bactericide is the biological bactericide of control postharvest disease of fruits and vegetables, as the biological bactericide of control grape postharvest disease.
5, the method for preventing and treating according to claim 4 is characterized in that, the active component of described biological bactericide is Cryptococcus laurentii (Cryptococcus laurentii).
6, the method for preventing and treating according to claim 5 is characterized in that, the use amount of described biological bactericide is 1 * 10
4-1 * 10
9CFU Cryptococcus laurentii (Cryptococcus laurentii)/ml bacterium liquid.
According to arbitrary described method of preventing and treating among the claim 1-6, it is characterized in that 7, described grape postharvest diseases is at least a in following three kinds of diseases: gray mold, anthracnose and white rot.
8, the control agent of a kind of grape postharvest diseases or fruit abscission is characterized in that, its active component comprises borate, is preferably the solubility borate, further is Boratex or potassium borate.
9, control agent according to claim 8 is characterized in that, described active component also comprises described biological bactericide, and described biological bactericide is the bacteria agent of control postharvest disease of fruits and vegetables, as the biological bactericide of control grape postharvest disease; The active component of described biological bactericide is Cryptococcus laurentii (Cryptococcus laurentii).
10, control agent according to claim 9 is characterized in that, described active component is described borate and described biological bactericide; In the described control agent, the proportioning of described borate and Cryptococcus laurentii (Cryptococcuslaurentii) is 2.62 * 10
-4-2.62 * 10
-3Mol borate ion: 1 * 10
6-1 * 10
11CFU Cryptococcus laurentii (Cryptococcus laurentii).
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101857843A (en) * | 2010-04-22 | 2010-10-13 | 浙江大学 | Method for improving prevention and control effects of biological control yeast on fruit diseases by induction and used culture medium |
| CN102210343A (en) * | 2010-04-02 | 2011-10-12 | 东北农业大学 | Cryptococcus albidus for fresh preservation of radish after picking |
| RU2472337C2 (en) * | 2011-03-29 | 2013-01-20 | Государственное научное учреждение Северо-Кавказский зональный научно-исследовательский институт садоводства и виноградарства Россельхозакадемии | Method of biologised protection of grape against diseases |
| CN103238633A (en) * | 2013-05-17 | 2013-08-14 | 中国科学院植物研究所 | Prevention solution for preharvest disease of Huping jujube and application thereof |
| CN103947746A (en) * | 2014-04-29 | 2014-07-30 | 中国科学院植物研究所 | Method for preserving grapes |
| CN106106721A (en) * | 2016-06-28 | 2016-11-16 | 浙江大学 | Induction fruit resistance controls the method for disease and preparation used |
| CN106167768A (en) * | 2016-07-15 | 2016-11-30 | 青岛农业大学 | The one pale yellow Cryptococcus of strain and in the application of the preventing and treating postharvest disease of fruits and vegetables such as blue berry |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1813549A (en) * | 2006-03-06 | 2006-08-09 | 江苏大学 | Method for preventing postharvest disease of strawberry |
| AR061582A1 (en) * | 2006-06-27 | 2008-09-03 | Innovacion Y Desarrollo S A | PRESERVANT PRODUCT FOR HARVESTED FRUITS AND VEGETABLES |
| CN101305748A (en) * | 2008-06-29 | 2008-11-19 | 中国海洋大学 | Active fruit and vegetable fresh-keeping film |
-
2009
- 2009-03-04 CN CN2009100788589A patent/CN101491273B/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210343A (en) * | 2010-04-02 | 2011-10-12 | 东北农业大学 | Cryptococcus albidus for fresh preservation of radish after picking |
| CN101857843A (en) * | 2010-04-22 | 2010-10-13 | 浙江大学 | Method for improving prevention and control effects of biological control yeast on fruit diseases by induction and used culture medium |
| RU2472337C2 (en) * | 2011-03-29 | 2013-01-20 | Государственное научное учреждение Северо-Кавказский зональный научно-исследовательский институт садоводства и виноградарства Россельхозакадемии | Method of biologised protection of grape against diseases |
| CN103238633A (en) * | 2013-05-17 | 2013-08-14 | 中国科学院植物研究所 | Prevention solution for preharvest disease of Huping jujube and application thereof |
| CN103238633B (en) * | 2013-05-17 | 2014-11-05 | 中国科学院植物研究所 | Prevention solution for preharvest disease of Huping jujube and application thereof |
| CN103947746A (en) * | 2014-04-29 | 2014-07-30 | 中国科学院植物研究所 | Method for preserving grapes |
| CN103947746B (en) * | 2014-04-29 | 2016-01-13 | 中国科学院植物研究所 | A kind of method of grape fresh-keeping |
| CN106106721A (en) * | 2016-06-28 | 2016-11-16 | 浙江大学 | Induction fruit resistance controls the method for disease and preparation used |
| CN106106721B (en) * | 2016-06-28 | 2020-03-17 | 浙江大学 | Method for controlling diseases by inducing fruit resistance and used preparation |
| CN106167768A (en) * | 2016-07-15 | 2016-11-30 | 青岛农业大学 | The one pale yellow Cryptococcus of strain and in the application of the preventing and treating postharvest disease of fruits and vegetables such as blue berry |
| CN106167768B (en) * | 2016-07-15 | 2019-02-19 | 青岛农业大学 | One plant of pale yellow Cryptococcus and its application in the prevention and treatment postharvest disease of fruits and vegetables such as blueberry |
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| CN101491273B (en) | 2011-11-09 |
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