CN104054729A

CN104054729A - Sterilization composition

Info

Publication number: CN104054729A
Application number: CN201410270446.6A
Authority: CN
Inventors: 卢小燕; 杨会营; 蒋勤桂; 刘晓娇; 吴勇
Original assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Current assignee: Yanhua Yongle (Leting) Biological Technology Co. Ltd.
Priority date: 2014-06-17
Filing date: 2014-06-17
Publication date: 2014-09-24
Anticipated expiration: 2034-06-17
Also published as: CN104054729B

Abstract

The invention relates to a sterilization composition which comprises two active components, namely pyraclostrobin and ningnanmycin, wherein the weight ratio of the two active components is (1 to 60)-(60 to 1). The composition has a large bacteriocidal spectrum, is high in efficiency, has an obvious synergism effect, and is safe to crops.

Description

A kind of bactericidal composition

Technical field

The invention belongs to technical field of pesticide, contain specifically the composite bactericide with synergistic effect of pyraclostrobin and Ningnanmycin.

Background technology

Pyraclostrobin (Pyraclostrobin) is novel low toxicity, efficient, wide-spectrum bactericide.This composition is mitochondrial respiratory inhibitor. by stoping electronics to shift, thereby reach the object of sterilization in cytochrome are synthetic.This composition has protection, treatment, blade infiltration conduction.Pyraclostrobin missible oil shows that through field control effectiveness test result powdery mildew of cucumber, downy mildew and banana freckle, leaf spot are had to good control efficiency.Through field efficacy, experiment shows cucumber, banana safety this composition, has no poisoning and occurs.

Ningnanmycin (ningnanmycin) is by the tunning that is isolated fungi at Ningnan County, Sichuan Province soil, is a kind of Cystosine nucleocide type antibiotics.This antibiotic is a kind of low toxicity, low-residual, without " three cause " and accumulation problems, novel pesticide free from environmental pollution, be 70% left and right to bacterial blight of rice relative control effect, high reaches 90%, effect of increasing production is 10%～20%, high reaches 35%, it is to wheat in addition, vegetables, the diseases prevention of the powdery mildews such as flowers, effect of increasing production is all very remarkable, to small round sclerotial disease of rice, Olive peacock's eye disease, shot hole and tobacco mosaic preventive effect are also fine, simultaneously at tobacco mosaic virus disease, tomato virus disease, pepper virus disease, rice seedling blight, root rot, stripe disease, alternaria leaf spot of apple, in powdery mildew of cucumber, obtain registration, this external control sclerotinia rot of colza, peronophythora litchi, other crop virosis, stem rot, climing rot, also large scale application in the multiple diseases such as powdery mildew.

Cucumber downy mildew is the more serious disease of cucumber, and seedling stage, strain all can be injured, mainly cause harm blade and stem, and tendril and bennet are injured less.Cucumber downy mildew optimum onset temperature is 16-24 DEG C, Seedling Stage morbidity, the irregular chlorisis yellowish-brown spot of the positive generation of cotyledon, strain morbidity, how, from forward position, greenhouse, there is water stain shape, light green fleck in morbidity strain before this middle and lower part blade reverse side, and front is aobvious, rear scab expands gradually, front exposed.This disease has larger impact to the output of cucumber, is badly in need of special efficacy, long medicament of lasting period to cucumber downy mildew on market.

Summary of the invention

The object of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection for preventing and treating cucumber downy mildew, compared with existing control medicament, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the bactericidal composition of pyraclostrobin and Ningnanmycin.

Utilize above-mentioned bactericidal composition, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, comprise suspending agent and water dispersible granules.

In above-mentioned bactericidal composition, the proportion of pyraclostrobin and Ningnanmycin is 1:60-60:1.

Bactericidal composition has following characteristics: the 1. advanced environmental protection of formulation, and environmental pollution is little, does not add organic principle in preparation; 2. bactericidal action is strong, is difficult for developing immunity to drugs; 3. two kinds of compositions are composite, have synergistic effect; 4. active ingredient is biogenic, strong to germ functionality.

Embodiment

For determining pyraclostrobin and two kinds of composite synergistic effects of active ingredient of Ningnanmycin, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:

First analyzed after the effect feature of two kinds of active ingredients, the raw object of surveying is decided to be Cuba artificial downy mildew (Pseudoperonospora cubensis) by we, and this Pseudomonas Mastigomycotina fungi, can cause the downy mildew of cucumber.

The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to cucumber downy mildew pathogen mycelial growth.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, the liquid that first the former medicine of 95% pyraclostrobin and the former medicine of 60% Ningnanmycin is made into series concentration taking methyl alcohol as solvent is for subsequent use, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour into and in the culture dish (Φ 90mm) of sterilizing, make band medicine flat board, every processing repeats for four times, to add the blank that is treated to of sterile water; With card punch (Φ 5mm) the picking bacterium of downy mildew of cucumber bacterium cake of sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 DEG C, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen ₅₀, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.

The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:

The theoretical toxicity index TTI=∑ (the toxicity index ATI × percentage of this pharmaceutically active ingredient in mixture of certain medicine) of mixture preparation.

It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition between 80-120 time.

Indoor biometrics test data sees the following form:

Table 1 pyraclostrobin, Ningnanmycin and the measurement result of different proportioning to cucumber downy mildew co-toxicity coefficient thereof

Analyze above-mentioned data, can obviously find out that the co-toxicity coefficient (CTC) of formula is all greater than 140, the prevention and control capability of cucumber downy mildew is had to the synergistic effect of different proportion in the time that pyraclostrobin and Ningnanmycin are composite in varing proportions.

By indoor biometrics evidence pyraclostrobin with two kinds of active ingredients of Ningnanmycin are composite has a good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 30.5% pyraclostrobin Ningnanmycin water dispersible granules

Example two, 31% pyraclostrobin Ningnanmycin suspending agent

Example three, 22% pyraclostrobin Ningnanmycin water dispersible granules

Example four, 18% pyraclostrobin Ningnanmycin suspending agent

Example five, 8% pyraclostrobin Ningnanmycin suspending agent

Example six, 12% pyraclostrobin Ningnanmycin suspending agent

Example seven, 33% pyraclostrobin Ningnanmycin water dispersible granules

Example eight, 15.5% pyraclostrobin Ningnanmycin suspending agent

Example nine, 36.6% pyraclostrobin Ningnanmycin suspending agent

Select above example to prevent and treat the field trial of cucumber downy mildew, determine the effect of formula control cucumber downy mildew; Simultaneously by with the Contrast on effect of pyraclostrobin and Ningnanmycin single dose, verify the synergistic effect after composite.Concrete test method is as follows:

8% pyraclostrobin Ningnanmycin suspending agent and other preparation do the test of pesticide effectiveness of field control cucumber downy mildew.Test site is Yang Ling area, Shaanxi Province, and the time is on May 25th, 2013, tests three repetitions of each processing, community randomized arrangement, and each community area is 50m ², adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method is: fixed 2 points of each community diagonal, and every some straight ahead is determined 20 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refers to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.

The grade scale of disease index:

0 grade: without scab;

1 grade: lesion area accounts for below 5% of whole leaf area;

3 grades: lesion area accounts for 6%～10% of whole leaf area;

5 grades: lesion area accounts for 11%～25% of whole leaf area;

7 grades: lesion area accounts for 26%～50% of whole leaf area;

9 grades: lesion area accounts for the more than 51% of whole leaf area.

Table 38% pyraclostrobin Ningnanmycin suspending agent and the preventive effect of different medicament to cucumber downy mildew

From above-mentioned data, can find out, pyraclostrobin and Ningnanmycin complex preparation 7 days preventive effects after medicine all exceed 70%, and pyraclostrobin single dose 7 days preventive effects after medicine are 46.91%, the preventive effect of Ningnanmycin single dose is 44.03%, compare pyraclostrobin and Ningnanmycin single dose, preventive effect difference between complex preparation and single dose is extremely remarkable, after visible two kinds of active ingredients are composite, has good synergistic effect.And after medicine 21 days, all complex preparations have all exceeded 72% to the preventive effect of cucumber downy mildew, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of cucumber downy mildew.Complex preparation can well improve drug effect, extends the lasting period, alleviates peasant's the amount of labour, has very high practicality.

Claims

1. a bactericidal composition, is characterized in that: active component is made up of pyraclostrobin and Ningnanmycin, and the weight ratio of described pyraclostrobin and Ningnanmycin is 1:60～60:1.

2. composition according to claim 1, is characterized in that: make suspending agent, water dispersible granules by active ingredient and auxiliary material.