CN106508937A - Bactericidal composition containing fenhexamid and Zhongshengmycin as well as application of bactericidal composition - Google Patents
Bactericidal composition containing fenhexamid and Zhongshengmycin as well as application of bactericidal composition Download PDFInfo
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- CN106508937A CN106508937A CN201611127180.5A CN201611127180A CN106508937A CN 106508937 A CN106508937 A CN 106508937A CN 201611127180 A CN201611127180 A CN 201611127180A CN 106508937 A CN106508937 A CN 106508937A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/22—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
- A01N37/24—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides containing at least one oxygen or sulfur atom being directly attached to the same aromatic ring system
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Abstract
The invention belongs to the technical field of pesticides and particularly relates to bactericidal composition containing fenhexamid and Zhongshengmycin as well as an application of the bactericidal composition. The pharmaceutical active ingredients of the bactericidal composition comprise fenhexamid and Zhongshengmycin in the weight ratio being (0.25-4):1. Fenhexamid and Zhongshengmycin are compounded, have a synergistic interaction instead of contradiction and have the control effect better than that of single agents. With the adoption of the composition, the dosage of a bactericide can be reduced, the efficiency is high, the drug resistance for diseases is avoided, the drug lasting period is prolonged, the sustainable development of the environment is facilitated, and the composition is safe to crops and the environment. The bactericidal composition has specific effects on botrytis, downy mildew, scab, early blight, late blight, black spot, anthrax, leaf cast, root rot and the like among crop, vegetable and fruit diseases.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of bactericidal composition containing fenhexamid and Zhongshengmycin and
Its application.
Background technology
Fenhexamid（Fenhexamid）For white powdery solid, fenhexamid is new amide-type systemic fungicide,
With the nothing such as existing bactericide benzimidazole, dicarboximide class, triazole type, aniline pyrimidine class, N- carbanilate classes
Cross resistance.Sclerotiniose, black spot to various gray molds and correlation has a good activity, such as preparation 50%WG, 50%SC, 50%
WP, but it is substantial amounts of research show its have uniqueness the mechanism of action, with oneself have bactericide benzimidazole, dicarboximide class,
The no interactions resistances such as triazole type, aniline pyrimidine class, N- carbanilate classes.To grape, xylocarp, strawberry, vegetables, oranges and tangerines,
The security of the crops such as ornamental plant, wheat.To crop, the mankind, Environmental security, it is that preferably comprehensive evil thing administers medication.It is anti-
Controlling object includes various gray molds, downy mildew, scab, early blight, late blight, black spot, anthracnose, defoliation, root rot
Deng.
Zhongshengmycin（1-N- glycosides base streptolidine base -2- amino -1B -2- deoxidation gulose amine）, it is that glucosides class resists
Raw element, aqua are dark brown, and pulvis is light yellow, free from extraneous odour.Belong to lower toxicity, low accumulation agricultural chemicals, category wide spectrum, efficiently, low toxicity,
Free of contamination agricultural antibiotic fungicide.Zhongshengmycin is alkaline, the water-soluble N- that a kind of load streptomycete Hainan mutation is produced
Glucosides class material, can suppress the synthesis of pathogen bacterium protein, and can make filamentous fungi deformity, suppress spore germination and kill
Spore, is generated by suppressing pathogenetic bacteria protein peptide bond, ultimately results in bacterial death, can stimulate protective plant protecting agent and wood in plant
The premise material of quality is generated, so as to improve the resistance against diseases of plant.Various bacteria and fungus-caused disease can be prevented and treated, such as
Bacterial blight of rice, cucumber bacterial angular leaf spot, bacterial wilt of tomato, green pepper shot hole, soft rot of cabbage, the bacillary epidemic disease of Kidney bean
Disease, apple fallen leaves spot disease, ring spot, anthracnose, root rotof flax etc..
Single bactericide is used for a long time in a large number, easily makes disease develop immunity to drugs, so as to cause dosage to increase, preventive effect drop
Low, the lasting period shortens, and is unfavorable for the sustainable development of environment.The compounding of medicament can not only delay resistance caused by single medicament institute
Generation, and fungicidal spectrum can be expanded, improve prevention effect.In prior art, the compounding of the element that mildews with regard to fenhexamid
At present at home and abroad there is not yet relevant report.
The content of the invention
It is an object of the invention to provide a kind of bactericidal composition containing fenhexamid and Zhongshengmycin, said composition sterilization
Spectrum is wide, and bactericidal effect is excellent.
Present invention also offers a kind of application containing fenhexamid and the bactericidal composition of Zhongshengmycin.
The technical scheme that adopted to achieve these goals of the present invention for：
The invention provides a kind of bactericidal composition containing fenhexamid and Zhongshengmycin, its active constituents of medicine is ring bacterium acid amides
And the weight ratio of Zhongshengmycin, fenhexamid and Zhongshengmycin is 0.25～4 1.
Further, the weight ratio of the optimization of the fenhexamid and Zhongshengmycin is 0.5～2:1.
Further, the optimized weight ratio of the fenhexamid and Zhongshengmycin is 1:1.
The preferred formulation of bactericidal composition that the present invention is provided is wettable powder；It is provided by the present invention containing fenhexamid
With the microbicide compositions of Zhongshengmycin, can be made into respectively with known auxiliary agent by method known in those skilled in the art
Plant any formulation for agriculturally allowing.
Further, the content of the active constituents of medicine is 15～80%.
Component of the bactericidal composition also including following percentage by weight：Urea 5%, sodium lignin sulfonate 0.9%, tartaric acid
0.1%th, polycarboxylate sodium 2%, ammonium sulfate 8.5%, balance of white carbon.
Present invention also offers a kind of mildew plain bactericidal composition in preventing and treating crop, vegetables, water with containing fenhexamid
Application in fruit disease evil.
Further, the disease is gray mold, downy mildew, black spot, anthracnose, defoliation, scab or late blight.
Beneficial effects of the present invention are：
1. the bactericide of the fenhexamid used by the present invention and two kinds of different mechanisms of action of Zhongshengmycin, mutual mixed synergies
Substantially, preventive effect is better than single dose；
2nd, composition of the invention can reduce bactericide usage amount, efficiency high, it is to avoid the resistance to the action of a drug of disease, extend medicine and hold
The effect phase, beneficial to the sustainable development of environment, to crop and Environmental security；
3. the microbicide compositions of the present invention be used to preventing and treating gray mold in crop, veterinary antibiotics disease, downy mildew, scab,
Early blight, late blight, black spot, anthracnose, defoliation, root rot etc. have special efficacy.
Specific embodiment
With reference to specific embodiment, the present invention is further illustrated, but the invention is not limited in this.
1 20% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（4:1）
Fill a prescription and be：Ring bacterium acid amides 16%, Zhongshengmycin 4%, urea 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, polycarboxylic acids
Sodium salt 2%, ammonium sulfate 8.5%, white carbon add to 100%.
Preparation method is：Raw material in formula is well mixed in mixer, Jing airslide disintegrating mills are crushed to 30 μm of particle diameter
Afterwards, amount of water 25% is mediated, carries out extruder grain, 40-50 DEG C of drying is obtained final product.
2 20% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（2:1）
Fill a prescription and be：Ring bacterium acid amides 13.4%, Zhongshengmycin 6.6%, urea 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, poly- carboxylic
Acid sodium-salt 2%, ammonium sulfate 8.5%, white carbon add to 100%.
Preparation method is with embodiment 1.
3 20% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（1:1）
Fill a prescription and be：Ring bacterium acid amides 10%, Zhongshengmycin 10%, urea 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, polycarboxylic acids
Sodium salt 2%, ammonium sulfate 8.5%, white carbon add to 100%.
4 20% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（1:2）
Fill a prescription and be：Ring bacterium acid amides 6.6%, Zhongshengmycin 13.4%, urea 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, poly- carboxylic
Acid sodium-salt 2%, ammonium sulfate 8.5%, white carbon add to 100%.
Preparation method is with embodiment 1.
5 20% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（1:4）
Fill a prescription and be：Ring bacterium acid amides 4%, Zhongshengmycin 16%, urea 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, polycarboxylic acids sodium
Salt 2%, ammonium sulfate 8.5%, white carbon add to 100%.
Preparation method is with embodiment 1.
6 20% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（1:1）
Fill a prescription and be：Ring bacterium acid amides 10%, Zhongshengmycin 10%, urea 5%, sodium lignin sulfonate 1%, polycarboxylate sodium 2%, ammonium sulfate
8.5%th, white carbon adds to 100%.
Preparation method is with embodiment 1.
Degradation after the bactericidal composition heat storage prepared in the embodiment, it may appear that part bulky grain.
7 15% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（1:1）
Fill a prescription and be：Ring bacterium acid amides 7.5%, Zhongshengmycin 7.5%, urea 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, poly- carboxylic
Acid sodium-salt 2%, ammonium sulfate 8.5%, white carbon add to 100%.
Preparation method is with embodiment 1.
8 40% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（1:1）
Fill a prescription and be：Ring bacterium acid amides 20%, Zhongshengmycin 20%, urea 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, polycarboxylic acids
Sodium salt 2%, ammonium sulfate 8.5%, white carbon add to 100%.
Preparation method is with embodiment 1.
9 80% ring bacterium acid amides of embodiment and Zhongshengmycin wettable powder（1:1）
Fill a prescription and be：Ring bacterium acid amides 40%, Zhongshengmycin 40%, urea 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, polycarboxylic acids
Sodium salt 2%, ammonium sulfate 8.5%, white carbon add to 100%.
Preparation method is with embodiment 1.
（One）Bactericidal composition prepared by above-described embodiment is to gray mold of cucumber indoor pharmacodynamic test
Indoor antibacterial measure is carried out using blade method, composition 5 is assembled into ratio, 50% ring bacterium acid amides and 1% Zhongshengmycin point respectively
The liquid that concentration is 100ppm, 50ppm, 10ppm, 1ppm, 0.1ppm is not configured to；Selection is sufficiently spread out, leaf age is consistent, carry
The healthy leaves of 1～2cm petioles, is placed in culture dish with wet cotton balls parcel petiole, and moisturizing is standby.Liquid is uniformly sprayed in
Vacuum side of blade, often processes 10 blades, is repeated 4 times.After liquid natural air drying, by process by each process vacuum side of blade upwards
Moisturizing in culture dish is emitted on after mark, and sets the process for active ingredient not being contained containing only solvent and surfactant do blank right
According to.The one side that diameter 5mm bacterial plaques have mycelia is inoculated in into process vacuum side of blade with inoculator.Ware lid is covered after inoculation, people is placed in
Work climate box, in 20 DEG C～22 DEG C, relative humidity 80%～90% and 12h alternation of light and darkness（Light intensity 5000Lux～10000 Lux）'s
Under the conditions of cultivate 7d.
Investigate depending on blank incidence.It is each once with right-angled intersection normal beam technique measurement lesion diameter with slide measure,
Average, unit is（mm）.
The fresh blade of cucumber simultaneously breaks into the leaf dish of a diameter of 1.5cm, is directly immersed in variable concentrations liquid, each concentration 10
Individual leaf dish；After drying, each leaf dish drips 60 μ l sporangia suspensions.21 DEG C of constant incubator moisturizing cultures are put into, are seen after 5d
Examine, the leaf dish number of scab occurs in record, calculate inhibiting rate, analyze EC50And its confidential interval.
Inhibiting rate（%）=（Control scab number-process scab number）/ control scab number × 100
Synergy type of the result of the test with the co-toxicity coefficient method evaluation of Sun Yunpei after mixed.
Toxicity index × the B of ratio+Bs of the toxicity index × A of the toxicity index theoretical value=A of mixture in mixture
Ratios of the toxicity index × C of the ratio+C in mixture in mixture
Criterion：Co-toxicity coefficient<80 is antagonism, is addition between 80-120,>It is synergistic effect when 120.
Suppression situation of 1 microbicide compositions of table to botrytis cinerea pers
By table as can be seen that weight ratio is 4:1、2:1、1:1、1:2 and 1:4 ring bacterium acid amides is with Zhongshengmycin composition to cucumber
In ash arrhizus bacteria indoor pharmacodynamic test, EC50Value is respectively 118.0512mg/L, 94.6543mg/L, 63.6814mg/L,
The EC of 95.5381mg/L, 96.3078mg/L, 50% ring bacterium acid amides wettable powder and 1% Zhongshengmycin wettable powder50Value
Respectively 163.9766mg/L and 122.4940mg/L, illustrates that ring bacterium acid amides is had to botrytis cinerea pers with Zhongshengmycin composition
There is a significant inhibitory action, and there is synergistic effect, and the 3rd to assemble than i.e. ring bacterium acid amides and Zhongshengmycin be 1:It is malicious altogether when 1
Coefficient reaches 220.11, and synergy is most obvious, illustrates that ring bacterium acid amides has suppression well with Zhongshengmycin composition to ash arrhizus bacteria
Effect, and both compound synergics are obvious.
（Two）Bactericidal composition preventing and treating gray mold of cucumber field control effectiveness test prepared by above-described embodiment 3
2 months-December in 2014 carries out gray mold of cucumber field control effectiveness test in Jinan Cui Zhaizhen Cai Ma villages booth, and arranging should
600 times of liquid of wettable powder, 800 times of liquid, 1000 times of liquid, medicament control is in 50% 1000 times of liquid of ring bacterium amide suspending agents and 1%
Raw rhzomorph 600 times of liquid of aqua, blank are sprayed for clear water.Respectively on spring stubble and two season of autumn stubble tomato.Cell random alignment,
Per plot area about 13m2, often process and be repeated 3 times, before spray, the 7th day after spray, investigate disease index and preventing and treating within the 14th day
Effect.
With reference to Ministry of Agriculture's agricultural chemicals field test criterion bactericidal agent for preventing and treating eliminating vegetable botrytis-cucumber, the investigation side of tomato pimento
Method：Sampled using 5 points per cell, per investigation 2-3 strain is investigated per plant of whole blades and whole fruits, calculates diseased fruit rate disease
Recorded according to following stage division point.
Leaf diseases stage division（In units of blade）：
0 grade：Disease-free spot；
1 grade：Monolithic leaf has scab 3；
3 grades：Monolithic leaf has scab 4-6；
5 grades：Monolithic leaf has scab 7-10；
7 grades：Monolithic leaf has scab 11-20, and part is intensive in flakes；
9 grades：Monolithic leaf has that scab is intensive to account for more than leaf area a quarter.
The killed stage division of cucumber fruits（In units of fruit）：
0 grade：Disease-free spot；
1 grade：Residual flower is without morbidity；
3 grades：Fall ill in areola portion；
5 grades：Scab length accounts for less than the 10% of fruit；
7 grades：Scab length accounts for the 11-25% of fruit；
9 grades：Scab length accounts for more than the 26% of fruit.
Statistical analysis formula is calculated:
Disease index=[Σ（The sick numbers of sheets at different levels × corresponding value of series）/（Investigation total number of sheets × 9）]×100
Rectifying efficacies (%)=[1-CK0×PT1/（CK1×PT0）]×100
In formula：CK0Disease index before blank district dispenser；
CK1Disease index after blank district dispenser；
PT0Disease index before chemicals treatment district dispenser；
PT1Disease index after chemicals treatment district dispenser.
2 microbicide compositions of table prevent and treat gray mold of cucumber field test disease index（Blade）
1. respectively process disease index and be 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
3 microbicide compositions of table are to gray mold of cucumber field test prevention effect
Each prevention effect that processes is 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
By table 2 and table 3 as can be seen that ring bacterium acid amides and Zhongshengmycin composition are to spring stubble and autumn stubble cucumber leaf portion gray mold
In preventing and treating, concentration be 600 times of liquid after administration the disease index of 14 days be respectively 11.82（Spring stubble cucumber）With 18.04（Autumn stubble is yellow
Melon）, prevention effect is respectively 89.31%（Spring stubble cucumber）With 91.47%（Autumn stubble cucumber）, concentration is 800 times of liquid after administration 14
It disease index is respectively 13.24（Spring stubble cucumber）With 22.08（Autumn stubble cucumber）, prevention effect is respectively 91.26%（Spring stubble
Cucumber）With 90.31%（Autumn stubble cucumber）, compared with the comparison medicament of peer-level, it is significantly better than 50% ring bacterium acid amides of comparison medicament
With 1% Zhongshengmycin, preventive effect significantly improves.
4 microbicide compositions of table prevent and treat gray mold of cucumber field test disease index（Fruit）
1. respectively process disease index and be 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
5 microbicide compositions of table are to gray mold of cucumber field test prevention effect（Fruit）
Each prevention effect that processes is 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
By table 4 and table 5 as can be seen that ring bacterium acid amides and Zhongshengmycin composition are to spring stubble and autumn stubble cucumber fruits gray mold
In preventing and treating, concentration be 600 times of liquid after administration the disease index of 14 days be respectively 9.08（Spring stubble cucumber）With 15.30（Autumn stubble is yellow
Melon）, prevention effect is respectively 89.95%（Spring stubble cucumber）With 82.34%（Autumn stubble cucumber）, concentration is 800 times of liquid after administration 14
It disease index is respectively 10.69（Spring stubble cucumber）With 19.34（Autumn stubble cucumber）, prevention effect is respectively 88.97%（Spring stubble
Cucumber）With 89.77%（Autumn stubble cucumber）, compared with the comparison medicament of peer-level, it is significantly better than 50% ring bacterium acid amides of comparison medicament
With 1% Zhongshengmycin, preventive effect significantly improves.Same detection is carried out to other embodiment.
（Three）Ring bacterium acid amides and Zhongshengmycin composition for preventing and controlling graw mold of tomato field control effectiveness test
2 months-December in 2014 carries out graw mold of tomato field control effectiveness test in Jinan Cui Zhaizhen Cai Ma villages booth, and arranging should
600 times of liquid of wettable powder, 800 times of liquid, 1000 times of liquid, medicament control is in 50% 1000 times of liquid of ring bacterium amide suspending agents and 1%
Raw rhzomorph 600 times of liquid of aqua, blank are sprayed for clear water.Respectively on spring stubble and two season of autumn stubble tomato.Cell random alignment,
Per plot area about 13m2, often process and be repeated 3 times, before spray, the 7th day after spray, investigate disease index and preventing and treating within the 14th day
Effect.
With reference to Ministry of Agriculture's agricultural chemicals field test criterion bactericidal agent for preventing and treating eliminating vegetable botrytis-cucumber, the investigation side of tomato pimento
Method：Sampled using 5 points per cell, per investigation 2-3 strain is investigated per plant of whole blades and whole fruits, calculates diseased fruit rate disease
Recorded according to following stage division point.
Leaf diseases stage division（In units of blade）：
0 grade：Disease-free spot；
1 grade：Monolithic leaf has scab 3；
3 grades：Monolithic leaf has scab 4-6；
5 grades：Monolithic leaf has scab 7-10；
7 grades：Monolithic leaf has scab 11-20, and part is intensive in flakes；
9 grades：Monolithic leaf has that scab is intensive to account for more than leaf area a quarter.
The killed stage division of tamato fruit（In units of fruit）：
0 grade：Disease-free spot；
1 grade：The morbidity of residual petal or column cap morbidity；
3 grades：Sepal rots or column cap morbidity spreads to areola portion；
5 grades：There is infiltration spot in areola portion without mould layer；
7 grades：There is mould layer in areola portion but does not expand to other positions；
9 grades：Mould layer expands to other positions of fruit.
Statistical analysis formula is calculated:
Disease index=[Σ（The sick numbers of sheets at different levels × corresponding value of series）/（Investigation total number of sheets × 9）]×100
Rectifying efficacies (%)=[1-CK0×PT1/（CK1×PT0）]×100
In formula：CK0Disease index before blank district dispenser；
CK1Disease index after blank district dispenser；
PT0Disease index before chemicals treatment district dispenser；
PT1Disease index after chemicals treatment district dispenser.
6 microbicide compositions of table prevent and treat graw mold of tomato field test disease index（Blade）
1. respectively process disease index and be 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
7 microbicide compositions of table are to graw mold of tomato field test prevention effect
Each prevention effect that processes is 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
By table 6 and table 7 as can be seen that ring bacterium acid amides and Zhongshengmycin composition are to spring stubble and autumn stubble tomato leaf portion gray mold
In preventing and treating, concentration be 600 times of liquid after administration the disease index of 14 days be respectively 14.68（Spring stubble tomato）With 20.09（Autumn stubble kind
Eggplant）, prevention effect is respectively 85.75%（Spring stubble tomato）With 75.14%（Autumn stubble tomato）, concentration is 800 times of liquid after administration 14
It disease index is respectively 16.10（Spring stubble tomato）With 25.50（Autumn stubble tomato）, prevention effect is respectively 77.40%（Spring stubble
Tomato）With 72.84%（Autumn stubble tomato）, compared with the comparison medicament of peer-level, it is significantly better than 50% ring bacterium acid amides of comparison medicament
With 1% Zhongshengmycin, preventive effect significantly improves.
8 microbicide compositions of table prevent and treat graw mold of tomato field test disease index（Fruit）
1. respectively process disease index and be 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
9 microbicide compositions of table are to graw mold of tomato field test prevention effect
1. respectively process prevention effect and be 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
By table 8 and table 9 as can be seen that ring bacterium acid amides and Zhongshengmycin composition are to spring stubble and autumn stubble tamato fruit gray mold
In preventing and treating, concentration be 600 times of liquid after administration the disease index of 14 days be respectively 12.37（Spring stubble tomato）With 18.59（Autumn stubble kind
Eggplant）, prevention effect is respectively 83.04%（Spring stubble tomato）With 80.84%（Autumn stubble tomato）, concentration is 800 times of liquid after administration 14
It disease index is respectively 13.79（Spring stubble tomato）With 22.63（Autumn stubble tomato）, prevention effect is respectively 76.66%（Spring stubble
Tomato）With 71.38%（Autumn stubble tomato）, compared with the comparison medicament of peer-level, it is significantly better than 50% ring bacterium acid amides of comparison medicament
With 1% Zhongshengmycin, preventive effect significantly improves.
（Four）Ring bacterium acid amides and Zhongshengmycin composition for preventing and controlling pear scab field control effectiveness test
By ring bacterium acid amides and Zhongshengmycin composition according to 1:1 weight ratio is made into wettable powder with known auxiliary agent.
In January, -2015 in March, 2014 carries out pear scab field control effectiveness test in Shandong Province's Laiyang City, arranges this wettable
Property 600 times of liquid of pulvis, 800 times of liquid, 1000 times of liquid, medicament control is raw bacterium in 50% 1000 times of liquid of ring bacterium amide suspending agents and 1%
600 times of liquid of plain aqua, blank are sprayed for clear water, totally 6 process.Test and 5 medicines are sprayed altogether in young fruit period in 2013, every 10
Its spray 1 time.5 medicines are sprayed altogether within 2014, sprayed 1 time every 10 days.Test is arranged using random district's groups, plot area about 24m2, often
Individual process is repeated 3 times, and carries out prevention effect investigation within 10 days in 2013 and 2014 after each last 1 spray.Per little during investigation
Area（Strain）20 leaves on current-year branch, 4 fruits are randomly selected by 5 orientation in East, West, South, North, per cell altogether
100 leaves of investigation, 20 fruits.
With reference to the investigation method of Ministry of Agriculture's agricultural chemicals field test criterion bactericidal agent for preventing and treating pear scab bactericide, occurring degree
Classification is as follows：
Blade stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than the 10% of whole leaf area；
3 grades：Lesion area accounts for the 10%～25% of whole leaf area；
5 grades：Lesion area accounts for the 26%～40% of whole leaf area；
7 grades：Lesion area accounts for the 41%～65% of whole leaf area；
9 grades：Lesion area accounts for more than the 65% of whole leaf area.
Fruit stage division：
0 grade：Disease-free spot on fruit；
1 grade：There is scab 1～2 on each fruit；
3 grades：There is scab 3～4 on each fruit；
5 grades：There is scab 5～6 on each fruit；
7 grades：There are 7～10 part scabs of scab to be connected on each fruit and account for fruit area 1/5th or so；
9 grades：There is scab more than 10 on each fruit, scab is connected and accounts for more than fruit area a quarter.
Statistical analysis formula is calculated：
Disease index={ ∑ ［ sick leaf (fruit) number × relative value of series at different levels ］/［ the total leaf (fruit) of investigation counts × 9 ］ } × 100
Pear scab prevention effect (%)=(disease index after disease index-treatment region dispenser after the dispenser of check plot)/process
Disease index after area's dispenser ］ × 100
Rectifying efficacies (%)=[1-CK0 × PT1/（CK1×PT0）]×100
In formula：Disease index before CK0 blank district dispensers；
Disease index after CK1 blank district dispensers；
Disease index before PT0 chemicals treatment district dispensers；
Disease index after PT1 chemicals treatment district dispensers.
10 composition for preventing and controlling pear scab field test effect of table
1. respectively process disease index and be 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
As can be seen from Table 10, during ring bacterium acid amides is prevented and treated to pear scab with Zhongshengmycin composition, concentration is 600 times of liquid
Disease index after dispenser is 2.72（2014）With 4.21（2015）, prevention effect is respectively 90.22%（2014）With
91.91（2015）, it is 3.77 that concentration is the disease index after 800 times of liquid dispensers（2014）With 7.27（2015）, preventing and treating
Effect is respectively 88.35%（2014）With 89.37（2015）, compared with the comparison medicament of peer-level, it is significantly better than control
50% ring bacterium acid amides of medicament and 1% Zhongshengmycin, preventive effect are significantly improved.
（Five）Ring bacterium acid amides is with Zhongshengmycin composition to early blight of tomato field control effectiveness test
By ring bacterium acid amides and Zhongshengmycin composition according to 1:1 weight ratio is made into wettable powder with known auxiliary agent.
2 months-December in 2014 carries out early blight of tomato field control effectiveness test in Jinan Cui Zhaizhen Cai Ma villages booth, if
Put 600 times of liquid of the wettable powder, 800 times of liquid, 1000 times of liquid, medicament control for 50% 1000 times of liquid of ring bacterium amide suspending agents with
1% 600 times of Zhongshengmycin aqua liquid, blank are sprayed for clear water.Cell random alignment, per plot area about 13m2, often process
It is repeated 3 times, before spray, the 7th day after spray, investigates disease index and prevention effect within the 14th day.
With reference to Ministry of Agriculture's agricultural chemicals field test criterion bactericidal agent for preventing and treating early blight of tomato and the investigation method of late blight：Per little
Area is sampled using 5 points, and per is investigated 2 plants, and 10 leaves are investigated in per plant point of upper, middle and lower, is accounted for the lesion area on per a piece of leaf
Being classified, while blade is investigated, investigation disease fruit number includes fallen fruits to the percentage of whole leaf area, represents anti-with diseased fruit rate
Control effect.
Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%-10% of whole leaf area；
5 grades：Lesion area accounts for the 11%-20% of whole leaf area；
7 grades：Lesion area accounts for the 21%-50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area；
Disease index=[Σ（The sick numbers of sheets at different levels × corresponding value of series）/（Investigation total number of sheets × 9）]×100
Rectifying efficacies (%)=[1-CK0×PT1/（CK1×PT0）]×100
In formula：CK0Disease index before blank district dispenser；
CK1Disease index after blank district dispenser；
PT0Disease index before chemicals treatment district dispenser；
PT1Disease index after chemicals treatment district dispenser.
11 composition for preventing and controlling early blight of tomato field test effect of table
1. respectively process disease index and be 3 repetition mean values；2. Notable difference test is carried out using " DMRT " method；3. difference
Conspicuousness compares using letter representation method, and all same letter persons represent that difference is not notable in same level.
As can be seen from Table 11, during ring bacterium acid amides is prevented and treated to spring stubble and autumn stubble early blight of tomato with Zhongshengmycin composition,
Concentration be 600 times of liquid after administration 14 days disease index be 4.08, prevention effect is 81.27%, and concentration is being applied for 800 times of liquid
After medicine, the disease index of 14 days is 5.37, and prevention effect is 74.77%, and compared with the comparison medicament of peer-level, it is right to be significantly better than
According to 50% ring bacterium acid amides of medicament and 1% Zhongshengmycin, preventive effect is significantly improved.
The a large amount of field tests of Jing prove that, when the bactericidal composition after present invention compounding is used compared with single dose, usage amount is reduced
25-30%。
Above-described embodiment is not limited by embodiment for the present invention preferably embodiment, but embodiments of the present invention
System, other any Spirit Essences and the changes, modification made under principle without departing from the present invention, combines, substitutes, simplifying and should be
Equivalence replacement mode, is included within protection scope of the present invention.
Claims (8)
1. a kind of bactericidal composition containing fenhexamid and Zhongshengmycin, it is characterised in that active constituents of medicine is ring bacterium acid amides
And the weight ratio of Zhongshengmycin, fenhexamid and Zhongshengmycin is 0.25～4 1.
2. bactericidal composition according to claim 1, it is characterised in that the weight ratio of the fenhexamid and Zhongshengmycin
For 0.5～2:1.
3. bactericidal composition according to claim 1 and 2, it is characterised in that the weight of the fenhexamid and Zhongshengmycin
Amount is than being 1:1.
4. bactericidal composition according to claim 3, it is characterised in that its formulation is wettable powder.
5. bactericidal composition according to claim 4, it is characterised in that the content of the active constituents of medicine is 15～
80%。
6. bactericidal composition according to claim 5, it is characterised in that also including the component of following percentage by weight：Urine
Element 5%, sodium lignin sulfonate 0.9%, tartaric acid 0.1%, polycarboxylate sodium 2%, ammonium sulfate 8.5%, balance of white carbon.
7. application of the bactericidal composition according to any one of claim 1～6 in preventing and treating crop, veterinary antibiotics disease.
8. application according to claim 7, it is characterised in that the disease is gray mold, downy mildew, scab, early epidemic
Disease, late blight, black spot, anthracnose, defoliation, root rot.
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