CN109566620B - Bactericidal composition, application thereof and bactericide - Google Patents
Bactericidal composition, application thereof and bactericide Download PDFInfo
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- CN109566620B CN109566620B CN201811641119.1A CN201811641119A CN109566620B CN 109566620 B CN109566620 B CN 109566620B CN 201811641119 A CN201811641119 A CN 201811641119A CN 109566620 B CN109566620 B CN 109566620B
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- bactericidal composition
- bactericide
- leaf spot
- copper complex
- oligosaccharide
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 54
- 239000000203 mixture Substances 0.000 title claims abstract description 32
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 22
- 230000001580 bacterial effect Effects 0.000 claims abstract description 33
- OICGFSUWXCJLCO-UHFFFAOYSA-N 1-ethylsulfonylsulfanylethane Chemical compound CCSS(=O)(=O)CC OICGFSUWXCJLCO-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000004936 Bromus mango Nutrition 0.000 claims abstract description 13
- 235000014826 Mangifera indica Nutrition 0.000 claims abstract description 13
- 235000009184 Spondias indica Nutrition 0.000 claims abstract description 13
- 229920001661 Chitosan Polymers 0.000 claims description 8
- 239000004480 active ingredient Substances 0.000 claims description 7
- 239000000839 emulsion Substances 0.000 claims description 3
- 239000004562 water dispersible granule Substances 0.000 claims description 3
- 230000000855 fungicidal effect Effects 0.000 claims description 2
- 240000007228 Mangifera indica Species 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 13
- 241001093152 Mangifera Species 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 7
- 244000052616 bacterial pathogen Species 0.000 abstract description 4
- 239000000575 pesticide Substances 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 9
- 238000000034 method Methods 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000001018 virulence Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 208000025865 Ulcer Diseases 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000000375 suspending agent Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 238000011179 visual inspection Methods 0.000 description 2
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- JNGZXGGOCLZBFB-IVCQMTBJSA-N compound E Chemical compound N([C@@H](C)C(=O)N[C@@H]1C(N(C)C2=CC=CC=C2C(C=2C=CC=CC=2)=N1)=O)C(=O)CC1=CC(F)=CC(F)=C1 JNGZXGGOCLZBFB-IVCQMTBJSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- -1 ethylicin compound Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
- A01N41/08—Sulfonic acid halides; alpha-Hydroxy-sulfonic acids; Amino-sulfonic acids; Thiosulfonic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
Abstract
The invention belongs to the field of pesticides, and particularly relates to a bactericidal composition, application thereof and a bactericide. The bactericidal composition provided by the invention comprises oligosaccharide-copper complex and ethylicin. According to the invention, the oligosaccharide-copper complex and ethylicin are compounded to be used as the bactericidal composition, so that the bactericidal composition has a very good control effect on bacterial angular leaf spot, especially mango bacterial angular leaf spot. The antibacterial effect test of mango bacterial angular leaf spot germs is carried out on the bactericidal composition provided by the invention, and the result shows that: effective intermediate concentration (EC)50) The lowest coefficient can reach 1.38, and the highest coefficient of Synergy (SR) can reach 2.48.
Description
Technical Field
The invention belongs to the field of pesticides, and particularly relates to a bactericidal composition, application thereof and a bactericide.
Background
Bacterial angular leaf spot, also known as bacterial black spot or ulcerative stain, is mainly found in the tip, leaves and fruits. The green-unchanged young shoot infected leaves are small black spots with the size of the needle, and then are expanded into small raised black spots, yellow halos are formed around the small raised black spots, the diseased spots are polygonal, the later diseased spots are fused into large irregular black spots, and the leaf stalks of the veins in the leaves generate longitudinal fissure spots. Needle-shaped black dots grow on the infected primordial pericarp of the fruit, and then the black dots expand into volcano-mouth-shaped dark brown ulcer spots, which are easy to induce the invasion of anthrax and pedicel rotting fungi.
The method is characterized in that typhoon and rainstorm frequently attack is caused in the late stage of fruit hanging of mangoes in coastal areas such as Guangdong, Guangxi, Hainan, Fujian and the like in China to the growth period of autumn tips, a large amount of mechanical injury is caused, pathogenic bacteria of bacterial angular leaf spot disease often invade by machine to cause morbidity, and serious economic loss can be caused to fruit growers if the prevention and the treatment are improper.
Disclosure of Invention
In view of the above, the present invention aims to provide a bactericidal composition, an application thereof and a bactericide, and the bactericidal composition provided by the present invention has a good control effect on bacterial angular leaf spot.
The invention provides a bactericidal composition which comprises oligosaccharide-copper complex and ethylicin.
Preferably, the oligosaccharide-copper complex is a chitosan oligosaccharide-copper complex.
Preferably, the mass ratio of the oligosaccharide-copper complex to the ethylicin is (0.1-10): 1.
preferably, the mass ratio of the oligosaccharide-copper complex to the ethylicin is (0.5-7): 1.
preferably, the mass ratio of the oligosaccharide-copper complex to the ethylicin is 0.6: 1.
The invention provides application of the bactericidal composition in the technical scheme in preventing and treating bacterial angular leaf spot.
Preferably, the bacterial angular leaf spot is mango bacterial angular leaf spot.
The invention provides a bactericide, wherein the active ingredient of the bactericide is the bactericidal composition as defined in any one of claims 1 to 5.
Preferably, the mass content of the active ingredient in the bactericide is 1-20 ppm.
Preferably, the dosage form of the bactericide is a suspending agent, an aqueous emulsion or water dispersible granules.
Compared with the prior art, the invention provides a bactericidal composition, application thereof and a bactericide. The bactericidal composition provided by the invention comprises oligosaccharide-copper complex and ethylicin. According to the invention, the oligosaccharide-copper complex and ethylicin are compounded to be used as the bactericidal composition, so that the bactericidal composition has a very good control effect on bacterial angular leaf spot, especially mango bacterial angular leaf spot. The antibacterial effect test of mango bacterial angular leaf spot germs is carried out on the bactericidal composition provided by the invention, and the result shows that: effective intermediate concentration (EC)50) The lowest coefficient can reach 1.38, and the highest coefficient of Synergy (SR) can reach 2.48.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a digital photographic image of bacterial colony growth provided by an example of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a bactericidal composition which comprises oligosaccharide-copper complex and ethylicin. Wherein the oligosaccharide-copper complex is preferably a chitosan oligosaccharide-copper complex; the mass ratio of the oligosaccharide-copper complex to the ethylicin is preferably (0.1-10): 1, more preferably (0.5 to 7): specifically, 1 may be 0.5:1, 0.6:1, 0.8:1, 1:1, 1.2:1, 1.5:1, 1.7:1, 2:1, 2.3:1, 2.5:1, 2.8:1, 3:1, 3.2:1, 3.5:1, 3.7:1, 4:1, 4.2:1, 4.5:1, 4.7:1, 5:1, 5.2:1, 5.5:1, 5.7:1, 6:1, 6.2:1, 6.5:1, 6.7:1 or 7: 1.
The sources of the oligosaccharide-copper complex and ethylicin are not particularly limited in the present invention, and they can be obtained by commercially available methods or by methods known to those skilled in the art.
The preparation method of the bactericidal composition is not particularly limited, and the raw materials are directly and uniformly mixed in proportion.
According to the invention, the oligosaccharide-copper complex and ethylicin are compounded to be used as the bactericidal composition, so that the bactericidal composition has a very good control effect on bacterial angular leaf spot, especially mango bacterial angular leaf spot. The antibacterial effect test of mango bacterial angular leaf spot germs is carried out on the bactericidal composition provided by the invention, and the result shows that: effective intermediate concentration (EC)50) The lowest coefficient can reach 1.38, and the highest coefficient of Synergy (SR) can reach 2.48.
The invention also provides application of the bactericidal composition in the technical scheme in preventing and treating bacterial angular leaf spot, in particular application in preventing and treating mango bacterial angular leaf spot. The application of the bactericidal composition provided by the invention has better prevention and treatment effects on bacterial angular leaf spot.
The invention also provides a bactericide, and the active ingredient of the bactericide is the bactericidal composition in the technical scheme. Wherein the mass content of the active component in the bactericide is preferably 1-20 ppm, and specifically can be 1ppm, 1.25ppm, 1.5ppm, 2ppm, 2.25ppm, 2.5ppm, 3ppm, 4ppm, 5ppm, 6ppm, 7ppm, 8ppm, 9ppm, 10ppm, 12ppm, 15ppm, 18ppm or 20 ppm; the dosage form of the bactericide comprises but is not limited to a suspending agent, an aqueous emulsion or water dispersible granules. In the present invention, other adjuvants or adjuvants contained in the fungicide are not particularly limited, and those skilled in the art may select them as appropriate according to the actual situation.
The bactericide provided by the invention takes the bactericidal composition as an active ingredient, so that the bactericide has excellent control effect on bacterial angular leaf spot, particularly mango bacterial angular leaf spot.
For the sake of clarity, the following examples are given in detail.
Examples
1. Test strains
Mango bacterial angular leaf spot (commercially available);
2. test agent
20 wt% of chitosan oligosaccharide-copper complex raw material, 5 wt% of ethylicin raw material;
3. determination of the bacteriostatic Effect
3.1 design of test protocol
Determining the bacteriostatic effect of the sample by adopting a flat plate dilution method; totally setting 5 chitosan oligosaccharide-copper complex and ethylicin compound samples with different proportions, setting 5 mass concentration gradients of active ingredients for each sample, wherein the mass concentration gradients are respectively 20ppm, 10ppm, 5ppm, 2.5ppm and 1.25ppm, additionally setting a single dose of chitosan oligosaccharide-copper complex, a single dose of ethylicin and control sample clear water, and totally setting 36 test samples, wherein each test sample is repeatedly measured for 3 times, and the detailed information of the test samples is shown in table 1 (the proportions of the chitosan oligosaccharide-copper complex and the ethylicin in table 1 are pure medicine proportions):
table 1 test sample information
3.2. Preparation of the respective test samples
Dissolving a test sample in sterilized water in a super clean bench to prepare a mother medicinal solution with the concentration of 1 wt%, fixing the volume to 10mL, and shaking up for later use.
3.3. Preparation of drug-containing plate
Cooling the freshly sterilized NA culture medium (50 mL/bottle) to about 45 ℃, sucking 0.5mL of mother liquor by using a pipette, adding the mother liquor into the culture medium, uniformly mixing, pouring the mixture into a flat plate, preparing medicament flat plates with the concentrations of 20ppm, 10ppm, 5ppm, 2.5ppm and 1.25ppm, and pouring 3 dishes of the culture medium in each bottle.
3.4. Inoculating bacteria
Preparing a bacterial liquid: by aseptic technique, the newly streaked bacteria are dissolved in 1mL of sterilized water, shaken, and diluted to 3X 106And (5) multiplying, for later use, removing 30uL of diluted bacterial liquid, adding into a medicine-containing culture medium to be coagulated, shaking up, finally placing in an incubator for culturing for 24h, and investigating the bacterial colony growth condition of the plate. The results are shown in FIG. 1, and FIG. 1 is a digital photograph of the growth of bacterial colonies provided by the examples of the present invention.
3.5. Data measurement
After 48h of incubation. Grading according to the growth condition of bacteria on the plate by adopting a visual method:
-: no growth occurs;
+: the growth of bacteria on the plate accounts for 1-10%;
++: the growth of bacteria on the plate accounts for 11-25%;
+++: the growth of bacteria on the plate accounts for 26-50%;
++++: the growth of bacteria on the plates was over 51%.
3.6. Analysis of test results (see tables 2 to 4)
TABLE 2 statistics of the growth of bacteria on the plates by visual inspection
TABLE 3 statistics of the number of bacterial colonies (unit: number) on the plate by visual inspection
TABLE 4 regression equation, EC50And SR results
| Regression equation | r | EC50 | x1 | x2 | SR (synergy) | |
| A | y＝4.1292+0.8086x | 0.8738 | 11.94 | 3.47 | 11.93 | 0.29 |
| B | y＝4.8603+0.3814x | 0.9960 | 2.32 | 3.43 | 2.3243 | 1.48 |
| C | y＝4.4142+1.2546x | 0.9942 | 2.93 | 3.36 | 2.9303 | 1.15 |
| D | y＝4.6349+0.4346x | 1 | 6.92 | 3.3 | 6.9196 | 0.48 |
| E | y＝4.6274+2.6436x | 0.9468 | 1.38 | 3.22 | 1.3 | 2.48 |
| F | y＝4.5380+0.8446x | 0.7543 | 3.52 | |||
| G | y＝4.4979+1.1251x | 1.0000 | 2.79 |
As can be seen from tables 2 and 3: the high concentration of 20ppm almost asepsis colony grows, A and F have a small amount of colony growth, it can be seen in the combination that E and D inhibit the virulence to mango bacterial angular leaf spot germ stronger, in the relatively high concentration of 20ppm, 10ppm, 5ppm asepsis colony growth, especially the combination D, E almost no bacterial growth of combination, E inhibits the bacterium better, better than single dose of ethylicin, from the combination, the higher the content of ethylicin, it inhibits the virulence stronger, it is difficult to find from the growth situation of visual observation colony, D, E inhibits the virulence stronger, and the colony number is less, especially the colony number of combination E is obviously less and control single dose of ethylicin.
As can be seen from table 4: the compound E has strong toxicity and has SP>1.5 has synergistic effect, and other compositions have no synergistic effect. The sequence of inhibiting virulence among the samples is as follows: e>B>G>C>F>D>A. The experiment finds that E is the best compound combination: chitosan oligosaccharide-copper complex: ethylicin is 3:5 (mass ratio), EC50Is 1.38, and has stronger effect than single dose of ethylicin.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (6)
1. A bactericidal composition comprising an oligosaccharide-copper complex and ethylicin;
the oligosaccharide-copper complex is a chitosan oligosaccharide-copper complex;
the mass ratio of the oligosaccharide-copper complex to the ethylicin is 0.6: 1.
2. Use of the fungicidal composition of claim 1 for the control of bacterial angular leaf spot.
3. Use according to claim 2, wherein the bacterial angular leaf spot is mango bacterial angular leaf spot.
4. A bactericide, characterized in that the active ingredient of the bactericide is the bactericidal composition according to claim 1.
5. The bactericide as claimed in claim 4, wherein the active ingredient is contained in the bactericide in an amount of 1 to 20ppm by mass.
6. The bactericide as claimed in claim 4, wherein the bactericide is in the form of suspension, aqueous emulsion or water dispersible granule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811641119.1A CN109566620B (en) | 2018-12-29 | 2018-12-29 | Bactericidal composition, application thereof and bactericide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811641119.1A CN109566620B (en) | 2018-12-29 | 2018-12-29 | Bactericidal composition, application thereof and bactericide |
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| Publication Number | Publication Date |
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| CN109566620A CN109566620A (en) | 2019-04-05 |
| CN109566620B true CN109566620B (en) | 2021-06-22 |
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| CN111084188B (en) * | 2019-12-30 | 2022-02-08 | 海南正业中农高科股份有限公司 | Composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof |
| CN112655711A (en) * | 2020-12-30 | 2021-04-16 | 江苏生久农化有限公司 | Mixed pesticide for preventing and controlling sanitary pests such as mosquitoes, flies and the like and use method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| EP2692232A1 (en) * | 2011-03-28 | 2014-02-05 | Bodegas Vega Sicilia, S.A. | Use of natural antifungal agents in order to prevent the infection of pruning wounds, grafts and lesions in woody plants by phytopathogenic fungi |
| CN103947671A (en) * | 2014-04-28 | 2014-07-30 | 牛赡光 | Chitosan oligosaccharide copper complex fungicide and application of chitosan oligosaccharide copper complex fungicide |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| EP2692232A1 (en) * | 2011-03-28 | 2014-02-05 | Bodegas Vega Sicilia, S.A. | Use of natural antifungal agents in order to prevent the infection of pruning wounds, grafts and lesions in woody plants by phytopathogenic fungi |
| CN103947671A (en) * | 2014-04-28 | 2014-07-30 | 牛赡光 | Chitosan oligosaccharide copper complex fungicide and application of chitosan oligosaccharide copper complex fungicide |
Non-Patent Citations (1)
| Title |
|---|
| 寡糖乙蒜素;农业部农药检定所;《农药登记公告》;20171031(第10期);134页 * |
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Denomination of invention: Bactericidal composition and its application and bactericide Effective date of registration: 20220304 Granted publication date: 20210622 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2019460000007 |
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Address after: Fuchang Village, Laocheng Development Zone, Chengmai County, Hainan Province, 571923 Patentee after: Hainan Zhengye Biotechnology Co.,Ltd. Address before: 571924 Fuchang village, Laocheng Development Zone, Chengmai County, Hainan Province Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. |