CN111084188B - Composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof - Google Patents
Composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof Download PDFInfo
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- CN111084188B CN111084188B CN201911391855.0A CN201911391855A CN111084188B CN 111084188 B CN111084188 B CN 111084188B CN 201911391855 A CN201911391855 A CN 201911391855A CN 111084188 B CN111084188 B CN 111084188B
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- ethylicin
- hymexazol
- chitosan oligosaccharide
- bacterial
- rice
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- LKPLKUMXSAEKID-UHFFFAOYSA-N pentachloronitrobenzene Chemical compound [O-][N+](=O)C1=C(Cl)C(Cl)=C(Cl)C(Cl)=C1Cl LKPLKUMXSAEKID-UHFFFAOYSA-N 0.000 description 1
- 229940044652 phenolsulfonate Drugs 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009165 saligot Nutrition 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229950009390 symclosene Drugs 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
- A01N41/08—Sulfonic acid halides; alpha-Hydroxy-sulfonic acids; Amino-sulfonic acids; Thiosulfonic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof, and particularly provides a pesticide composition containing chitosan oligosaccharide, wherein the chitosan oligosaccharide is the amino oligosaccharide, the hymexazol and the ethylicin, the pesticide composition contains the chitosan oligosaccharide, the hymexazol and the ethylicin, and the weight ratio of the chitosan oligosaccharide, the hymexazol and the ethylicin is 1-5:2-30: 5-40. The pesticide composition containing chitosan oligosaccharide, hymexazol and ethylicin provided by the invention can effectively control soil-borne diseases and bacterial diseases, and particularly has excellent effects in preventing and treating the soil-borne diseases and the bacterial diseases of rice in the seedling stage.
Description
Technical Field
The invention relates to the field of agriculture, in particular to a composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof.
Background
With the continuous development of the protected area and the continuous cultivation of crops with high added value, the problem of soil-borne diseases becomes more and more prominent and becomes an important factor for seriously restricting the development of the protected area. Bacterial and fungal diseases also present a tendency to develop more and more uncontrollable, especially in areas where part of the crop cultivation methods are unscientific and the planting is sparse to manage. Among them, the rice damping-off is the most likely soil-borne disease in the rice seedling bed stage, for example, the bud rot occurs before emergence of seedlings or immediately after emergence of the seedlings, the sprouts or the roots of the seedlings turn brown, and the diseased buds are twisted and rotten to die. A mould layer is grown on the base of the seeds or buds. For example, needle rot often occurs in the seedling stage from the needle stage to the 2-leaf stage, the heart leaves of the seedling are withered and yellow, the leaves are not unfolded, the base is browned, brown spots are generated on the leaf sheath sometimes, and the diseased roots are gradually brownish yellow. The root junction of the seeds and the seedlings has a mildew layer, the stem base is weak and easy to break, and the seedlings in the seedling bed are often clustered, become flaked and die. For example, yellow withering occurs around the 2.5 leaf stage of the seedling, the diseased seedling leaves do not spit water, the leaves are withered and yellow, wilting, and the diseased seedling leaves rapidly expand outwards in a cave-like manner, the base and the root of the seedling are pulled off at very late, and the leaves are strung. In a sunny day, the seedlings quickly appear withered, and the heart leaves and upper leaves are "tressed". The color of the seedling is dark green, and finally the whole plant wilts. The rice damping-off also belongs to soil-borne diseases and is caused by infection of various pathogenic bacteria, and pathogenic bacteria of the rice damping-off, such as rhizoctonia and fusarium, are widely existed in soil and are weak parasitic bacteria, and can generally survive in water or soil. The pathogenicity of the germs is not strong, the germs are not suitable for infecting robust seedlings generally, and various weak parasitic bacteria can enter and spread in a conquering way only after the seedlings grow weakly and the resistance is reduced due to bad weather and improper management. Therefore, poor quality, weak growth and poor resistance to diseases and adverse stresses of seedlings are the direct causes of the occurrence of damping off. The bacterial wilt of rice mostly occurs before and after the 3-leaf stage of dry-raised rice seedlings, the leaf tips of the diseased seedlings physiologically do not spit water, the leaves are grey green and wilting, the seedbed forms holes, the seedling bases and roots are easily broken, and the leaves are strung. In a sunny day, the seedlings quickly appear withered, and the heart leaves and upper leaves are strung. The color of the seedling is dark green, and finally the whole plant wilts.
The rice bacterial diseases mainly comprise bacterial blight, bacterial leaf streak and bacterial basal rot. The pesticide for preventing and treating the bacterial blight of the rice mainly comprises bismerthiazol, thiediazole copper, nonyl phenolsulfonate copper, thiazole zinc, zhongshengmycin, streptomycin, chlorobromoisocyanuric acid, iodine oligoate, fluorobenzyl oxazole sulfone and the like. The chemical prevention and control effect is influenced by the characteristics of the medicament, the application technology, the climatic conditions and other factors. The chemical control difficulty of the rice bacterial leaf blight is that the existing bactericide has short duration, the bacterial leaf blight has long occurrence period, and the bactericide is more likely to be outbreak in strong wind and heavy rain, so that the bactericide needs to be applied for many times, and the bactericide needs to be applied in a supplementary manner after disastrous weather. Bacterial streak disease can be treated by trichloroisocyanuric acid and dithiocyano-methane to kill germs carried in seeds, thereby achieving the purpose of controlling diseases; the preparation for preventing bacterial blight can be used for preventing bacterial streak, such as thiazole and antibiotic. In addition, the chemical control effect of bacterial leaf streak is also influenced by weather factors, and the aim of controlling diseases can be achieved only by continuously and repeatedly spraying the pesticide. At present, the disclosed prevention and control of bacterial basal rot is based on prevention, and the medicament is sprayed before or at the early stage of the disease, so that the effect is good by using 3 percent of benziothiazolinone wettable powder and 40 percent of Chunlei thiazole zinc suspending agent.
The pesticide active ingredient used in the invention is amino-oligosaccharide, also called chitosan oligosaccharide, chemical name is oligo-D glucosamine, the pesticide belongs to a bactericide with disease-resistant effect extracted by microorganism metabolism, and has the effects of immunizing and killing various fungi, bacteria and viruses by inducing plant to generate disease resistance and inhibiting gene expression of pathogenic bacteria. The chitooligosaccharide can also stimulate growth, so that the plant is strong and the root system is developed, and the effects of increasing the yield and improving the quality are achieved. The chitosan oligosaccharide may be prepared by any method, for example, the following method: chitosan with deacetylation degree above 70% is prepared into mixture of oligomeric chitosan with polymerization degree of 2-30 by physical degradation method, chemical degradation method, enzyme degradation method, glycosyl transfer method, composite degradation method, etc. Or extracting chitin from fungal cell wall or insect body wall, and preparing mixture of oligochitosan with polymerization degree of 2-30 by physical degradation method, chemical degradation method, enzyme degradation method, glycosyl transfer method, composite degradation method, etc. Alternatively, a mixture of polymers having a degree of polymerization of 2 to 30 is synthesized chemically from glucosamine via 1, 4-glycosidic bonds. At present, the chitosan oligosaccharide is a mixture of high molecular polymers of different polymers, namely, the chitosan oligosaccharide is not in the form of chitosan oligosaccharide with a single polymerization degree, and chitosan oligosaccharide monosaccharide with each polymerization degree can exist. Therefore, the chitosan oligosaccharide of the present invention can be prepared by any method or a commercially available product prepared by the above preparation method, as long as it is a mixture of high molecular polymers having a polymerization degree of 2 to 30 and an average molecular weight of 1000-.
Hymexazol is a broad-spectrum systemic fungicide. High efficiency, low toxicity, and obvious preventing and treating effect on various plant fungal diseases, such as rhizoctonia rot, root rot, withered and dead seedling caused by fusarium, saprophytic bacteria, pythium, rhizoctonia, etc. Is widely applicable to the crop diseases of rice, wheat, cotton, beet, tobacco, vegetables, nursery stocks, fruit trees, cereals, oilseed plants, melons, lawns, flowers and other crops caused by the pathogenic fungi. Strong soil sterilization and seed disinfection, and special effects on various soil-borne diseases; promoting the growth of crops and the development of root systems, and resisting various physiological diseases in seedling stage. The plant growth promoting agent has systemic property and conductivity, can be directly absorbed by plant roots and enter the plant body, and can move very quickly, the plant growth promoting agent moves to the stem part for 24 hours only after moving in the root system for 3 hours and moves to the whole plant body, and metabolites of the plant growth promoting agent in the plant body are two kinds of glucoside, so that the plant growth promoting agent has the effect of promoting the growth of plants. The hymexazol can improve the pesticide effect in soil, most of bactericides are used for soil disinfection, are easily absorbed by soil and have the tendency of reducing the pesticide effect, and the hymexazol still has bactericidal activity within two weeks. Hymexazol can promote plant growth, and the branching of root, the number of root hair and the activity of root are increased.
Ethylicin (ethylicin) is a broad-spectrum bactericide which is developed by our country, also called as an antibacterial agent 402, Kangjia and the like, and the main dosage forms are 20% of hypertonic ethylicin missible oil, 30% of ethylicin wettable powder, 80% of ethylicin missible oil, 90% of ethylicin crude oil and the like. Ethylicin is a plant bionic pesticide, and is an allicin extract and an ethyl homolog of allicin. Can effectively inhibit cotton verticillium wilt, fusarium wilt and verticillium wilt; rice blast, bacterial leaf blight, bakanae disease, seedling rot and sheath blight; the disease is characterized by comprising the following components of corn big and small leaf spot, yellow leaf, wheat scab, stripe disease, stinking smut, watermelon slow blight, watermelon seedling disease, cucumber seedling blight, gray mold, scab, downy mildew, Chinese cabbage soft rot, ginger blast, tomato gray mold, bacterial wilt, pepper blight and various diseases on crops such as strawberry, bighead atractylodes rhizome, ginseng, banana, apple, grape, pear tree, tea, water chestnut, flowers, peanut, soybean, sesame and the like, and the effect is remarkable.
Among the published documents, documents for controlling soil-borne diseases such as CN1260956A disclose a broad-spectrum fungicide mixed formulation for controlling soil-borne diseases, which employs a composition prepared from quintozene and sodium diuron, triadimefon, hymexazol, iprodione, etc. for controlling soil-borne diseases of crops such as vegetables, melons, strawberries, hemp, nursery stocks, tobacco, cotton, soybeans, peanuts, etc. For example, CN109497072A discloses a composition for controlling field soil-borne diseases of lily and a use method thereof, which adopts hymexazol, carbendazim and continuous cropping No. 1 to control field soil-borne diseases. For example, CN108821860A discloses a bacterial preparation capable of alleviating soil-borne diseases, promoting rooting and resisting continuous cropping, which uses a bacterial preparation prepared from humic acid, chitooligosaccharide, spores/gram of bacillus licheniformis, naphthylacetic acid, indolebutyric acid and other raw materials to alleviate soil-borne diseases and promote crops to root quickly. For example, CN108541720A discloses an application of ethylicin in controlling plant soil-borne diseases, wherein the ethylicin is mixed with tobacco stem waste activated carbon to prepare a mixture for controlling the plant soil-borne diseases.
At present, effective medicaments for preventing and treating rice seedling-stage soil-borne diseases and rice bacterial diseases are few in the market, and the invention develops products for solving the technical problems.
Disclosure of Invention
In order to solve the technical problems, the invention provides a pesticide composition containing chitosan oligosaccharide, hymexazol and ethylicin, which is characterized by comprising the chitosan oligosaccharide, the hymexazol and the ethylicin, wherein the weight ratio of the chitosan oligosaccharide, the hymexazol and the ethylicin is 1-5:2-30: 5-40.
Preferably, in the pesticide composition, the weight ratio of the chitosan oligosaccharide, the hymexazol and the ethylicin is 1-5:5-15:10-20, and the weight ratio of the chitosan oligosaccharide, the hymexazol and the ethylicin is 1-5:7-15: 10-18.
Preferably, in the pesticide composition, the sum of the weight of the chitosan oligosaccharide, the hymexazol and the ethylicin accounts for 2 to 80 percent of the weight of the pesticide composition; preferably, the sum of the weight of the chitosan oligosaccharide, the hymexazol and the ethylicin accounts for 5 to 80 percent of the weight of the pesticide composition, preferably, the sum of the weight of the chitosan oligosaccharide, the hymexazol and the ethylicin accounts for 10 to 60 percent of the weight of the pesticide composition, and preferably, the sum of the weight of the chitosan oligosaccharide, the hymexazol and the ethylicin accounts for 20 to 30 percent of the weight of the pesticide composition.
Preferably, in the pesticide composition, the pesticide composition is a pesticide preparation, and the pesticide preparation is one of granules, microcapsules, an aqueous emulsion, a suspending agent, water dispersible granules, wettable powder and a microemulsion.
The invention also provides application of the pesticide composition in preventing and treating plant diseases, which is characterized in that the plant diseases are plant fungal diseases or plant bacterial diseases.
Preferably, the plant bacterial disease is rice bacterial leaf streak, rice bacterial leaf blight, rice bacterial basal rot, rice bacterial brown strip disease, rice bacterial brown spot, potato bacterial wilt, potato soft rot, potato black shank, potato ring rot, sweet potato blast, corn stem rot, wheat glume smut, cassava bacterial wilt, citrus canker, Shatian pomelo canker, pear root canker, pear fire blight, peach bacterial perforation, apple root canker, pineapple heart canker, loquat bud blight, loquat cancerous protuberance, walnut black spot, kiwi canker, mango bacterial black spot or fruit tree bacterial root canker.
Preferably, the plant bacterial disease is one or more of bacterial blight of rice, bacterial leaf streak of rice or bacterial basal rot of rice.
Preferably, the fungal disease of the plant is rot, anthracnose, ring spot, powdery mildew, black spot or dry rot, and the plant is lawn grass, tomato, potato, leek, ginger, tobacco, pepper, camellia oleifera, wheat, rice, corn, spinach, kohlrabi, Chinese cabbage, rape, garlic, shallot, cucumber, balsam pear, loofah, watermelon, cantaloupe, wax gourd, celery, sugar beet, peanut, soybean, green soybean, pea, kidney bean, strawberry, blueberry, sugarcane, pear, banana, papaya, mango, rubber, coconut, citrus, kiwi, pineapple, apple, jujube, persimmon, grape, cherry, lychee, peach, plum, apricot, marigold, monthly rose, camellia, tea tree or coconut.
The invention also provides application of the pesticide composition in preventing and treating soil-borne diseases of plants.
Preferably, the plant soil-borne disease is rice damping off or rice bacterial wilt.
The invention also provides an application method of the pesticide composition, which is characterized in that the pesticide composition is diluted and then is subjected to seed soaking, root irrigation, spraying, pouring splashing, furrow application, toxic soil mixing or hole application, and preferably, the dilution multiple is 100-5000 times.
The invention has the beneficial effects that:
the pesticide composition containing chitosan oligosaccharide, hymexazol and ethylicin provided by the invention can effectively control soil-borne diseases and bacterial diseases, and particularly has excellent effects in preventing and treating the soil-borne diseases and the bacterial diseases of rice in the seedling stage.
In the pesticide composition, the chitosan oligosaccharide, the hymexazol and the ethylicin have mutual synergistic effect in preventing and treating the rice soil-borne diseases and the rice bacterial diseases, can realize crop safety while realizing reduced pesticide application, and has important significance in food safety because green pesticides are mainly used in the pesticide composition.
Detailed Description
In order to understand the present invention, the following formulation examples and test examples further illustrate the present invention, but do not limit the present invention.
First, preparation examples
The following preparations were all prepared by the conventional preparation method of the preparations from the high-tech company Limited in the Hainan Zhengye.
Agent Y01: 5% chitosan oligosaccharide aqueous solution
Agent Y02: 30% hymexazol water aqua
Agent Y03: 80% ethylicin emulsifiable concentrate
Agent Y04: 5% chitosan oligosaccharide and 10% hymexazol water aqua
Agent Y05: aqueous solution of 1% chitosan oligosaccharide and 8% hymexazol
Agent Y06: 2% chitosan oligosaccharide and 10% hymexazol water aqua
Agent Y07: suspension containing 10% hymexazol and 15% ethylicin
Agent Y08: suspension containing 10% hymexazol and 13% ethylicin
Agent Y09: 8% hymexazol and 11% ethylicin suspension
Agent Y10: 10% hymexazol and 18% ethylicin suspension
Agent Y11: 5% chitosan oligosaccharide and 15% ethylicin suspending agent
Agent Y12: 5% chitosan oligosaccharide and 13% ethylicin suspending agent
Agent Y13: 1% chitosan oligosaccharide and 11% ethylicin suspending agent
Agent Y14: 2% chitosan oligosaccharide and 18% ethylicin suspending agent
Agent Y15: suspending agent containing 5% of chitosan oligosaccharide, 10% of hymexazol and 15% of ethylicin
Agent Y16: suspending agent containing 5% of chitosan oligosaccharide, 10% of hymexazol and 13% of ethylicin
Agent Y17: suspending agent containing 1% of chitosan oligosaccharide, 8% of hymexazol and 11% of ethylicin
Agent Y18: suspending agent containing 2% of chitosan oligosaccharide, 10% of hymexazol and 18% of ethylicin
II, bioassay example:
bioassay example 1 control of soil-borne diseases in Rice seedling stage
The test field is a demonstration field in Wuchang county of Heilongjiang province, and the seedling blocks in the control area are three in number, and each in number is 1 square meter. The test of each medicament area is repeated for three times, each time is repeated for 1 square meter, the application time is root irrigation application in two-leaf period, the application concentration is as shown in the following tables 1 and 2, and the disease condition is counted during application. In order to realize the application concentration, the medicine Y01 is diluted 1000 times, the medicine Y02 is diluted 3000 times, the medicine Y03 is diluted 5300 times, the medicines Y04, Y07, Y11 and Y15 are respectively diluted 1000 times before application, and the root irrigation application is carried out after the dilution.
The statistical time of the control effect is 3 days before the rice seedlings are transplanted. The statistical method of the control effect of each repetition is a five-point sampling method, the midpoint of the diagonal line of each seedling planting block is firstly determined as a central sampling point, then four points with the same distance with the central sampling point are selected as sampling points on the diagonal line, and each sampling point samples 20 plants to count the control condition of the disease.
The disease condition base number and the disease condition after application are investigated by taking a plant as a unit, and a disease plant with the symptoms of damping off and bacterial wilt is counted as a disease-causing plant. Because the diseases of the damping-off and the bacterial wilt of rice seedlings are mainly prevented, and once the diseases are difficult to recover, the calculation method of the disease index before pesticide application and the disease index after pesticide treatment comprises the following steps:
the calculation method of the prevention and treatment effect comprises the following steps:
when the disease index before drug application is zero, the calculation method of the prevention and treatment effect is as follows:
the control effects on damping-off and bacterial wilt in the rice seedling stage are shown in the following tables 1 and 2.
TABLE 1 preventive and therapeutic effects of the agents on seedling blight of rice
| Medicament | Active ingredient | Application concentration (ppm) | Control effect (%) | Theoretical control effect (%) |
| Medicament Y01 | Chitosan oligosaccharide | 50 | 36.8 | -- |
| Medicament Y02 | Hymexazol | 100 | 62.3 | -- |
| Medicament Y03 | Ethylicin | 150 | 41.2 | -- |
| Medicament Y04 | Chitosan oligosaccharide and hymexazol | 50+100 | 63.4 | 76.2 |
| Medicament Y07 | Hymexazol + ethylicin | 100+150 | 60.4 | 77.8 |
| Medicament Y11 | Chitosan oligosaccharide + ethylicin | 50+150 | 68.5 | 62.8 |
| Medicament Y15 | Chitosan oligosaccharide, hymexazol and ethylicin | 50+100+150 | 91.2 | 75.0 |
As can be seen from the table 1, the compound composition of chitosan oligosaccharide, hymexazol and ethylicin has a good effect of preventing and treating the rice seedling blight, the three medicaments realize a synergistic effect, and the synergistic effect solves the problem of preventing and treating the rice seedling blight compared with the use effect of compounding two active ingredients.
TABLE 2 preventive and therapeutic effects of the agents on rice bacterial wilt
| Medicament | Active ingredient | Application concentration (ppm) | Control effect (%) | Theoretical control effect (%) |
| Medicament Y01 | Chitosan oligosaccharide | 50 | 56.7 | -- |
| Medicament Y02 | Hymexazol | 100 | 46.2 | -- |
| Medicament Y03 | Ethylicin | 150 | 65.5 | -- |
| Medicament Y04 | Chitosan oligosaccharide and hymexazol | 50+100 | 79.3 | 76.7 |
| Medicament Y07 | Hymexazol + ethylicin | 100+150 | 81.0 | 81.4 |
| Medicament Y11 | Chitosan oligosaccharide + ethylicin | 50+150 | 88.7 | 85.1 |
| Medicament Y15 | Chitosan oligosaccharide, hymexazol and ethylicin | 50+100+150 | 97.3 | 91.8 |
As can be seen from Table 2, the agent Y15 containing chitosan oligosaccharide, hymexazol and ethylicin can effectively prevent and treat rice bacterial wilt. Particularly, compared with the prevention effect of a single preparation and the prevention effect of a binary compound preparation, the composition can be used for soil root irrigation and can effectively prevent and treat the rice bacterial wilt.
Bioassay example 2 prevention and treatment of bacterial disease bacterial blight of Rice
The control test of bacterial disease bacterial leaf blight of rice is carried out in late rice fields in Hubei, the number of control test fields is three, each is 20 square meters, each medicament test field is three, each is 20 square meters, the medicament application time is spray application in a tillering stage, the medicament application concentration is shown in the following table 3, and the disease indexes of bacterial leaf blight of the control test fields and the medicament test fields are investigated and counted simultaneously during medicament application. In order to realize the application concentration, before application, the medicine Y01 is diluted by 500 times, the medicine Y02 is diluted by 1500 times, the medicine Y03 is diluted by 3200 times, and the medicine Y04, the medicine Y08, the medicine Y12 and the medicine Y16 are respectively diluted by 500 times. Spraying the diluted mixture for once, and examining the disease index of bacterial leaf blight of the bacterial leaf blight at the heading stage.
Disease grading, disease index statistics and prevention and control effect statistics are carried out according to a conventional grading method and a conventional statistical method of rice bacterial leaf blight.
TABLE 3 control Effect on bacterial leaf blight of Rice
| Medicament | Active ingredient | Application concentration (ppm) | Controlling effect | Theoretical prevention and treatment effect |
| Medicament Y01 | Chitosan oligosaccharide | 100 | 23.1 | -- |
| Medicament Y02 | Hymexazol | 200 | 35.4 | -- |
| Medicament Y03 | Ethylicin | 250 | 71.2 | -- |
| Medicament Y04 | Chitosan oligosaccharide and hymexazol | 100+200 | 51.2 | 50.3 |
| Medicament Y08 | Hymexazol + ethylicin | 200+250 | 80.7 | 81.4 |
| Medicament Y12 | Chitosan oligosaccharide + ethylicin | 100+250 | 80.6 | 77.9 |
| Medicament Y16 | Chitosan oligosaccharide, hymexazol and ethylicin | 100+200+250 | 93.8 | 85.2 |
From the results in table 3, it can be seen that the ternary combination of chitosan oligosaccharide, hymexazol and ethylicin is synergistic and can effectively prevent and treat bacterial blight of rice.
Bioassay example 3 prevention and treatment of bacterial leaf streak of Rice
The control test of the bacterial leaf streak of the rice is carried out in the late rice field in Hubei, the number of the control test fields is three, each is 20 square meters, each medicament test field is three, each is 20 square meters, the medicament application time is spray application in the tillering stage, the medicament application concentration is shown in the following table 4, and the disease indexes of the bacterial leaf streak of the control test fields and the medicament test fields are counted simultaneously during the medicament application. In order to realize the application concentration, before application, the medicine Y01 is diluted 2500 times, the medicine Y02 is diluted 2000 times, the medicine Y03 is diluted 3650 times, and the medicine Y05, the medicine Y09, the medicine Y13 and the medicine Y17 are respectively diluted 500 times. Spraying the medicine after dilution, wherein the medicine application frequency is once, and the disease index of the bacterial leaf streak is investigated at the heading stage. Disease grading, disease index statistics and prevention and control effect statistics are carried out according to a conventional grading method and a statistical method of the bacterial leaf streak of the rice.
TABLE 4 prevention and treatment effects on bacterial leaf streak of rice
| Medicament | Active ingredient | Application concentration (ppm) | Controlling effect | Theoretical prevention and treatment effect |
| Medicament Y01 | Chitosan oligosaccharide | 20 | 13.7 | -- |
| Medicament Y02 | Hymexazol | 150 | 48.6 | -- |
| Medicament Y03 | Ethylicin | 220 | 63.5 | -- |
| Medicament Y05 | Chitosan oligosaccharide and hymexazol | 20+150 | 56.8 | 55.6 |
| Medicament Y09 | Hymexazol + ethylicin | 150+220 | 83.5 | 81.2 |
| Medicament Y13 | Chitosan oligosaccharide + ethylicin | 20+220 | 70.2 | 68.5 |
| Medicament Y17 | Chitosan oligosaccharide, hymexazol and ethylicin | 20+150+220 | 92.4 | 85.8 |
From the results in table 4, it can be seen that the ternary complex formulation of chitosan oligosaccharide, hymexazol and ethylicin is synergistic in preventing and treating bacterial leaf streak of rice, and can effectively prevent and treat bacterial leaf streak of rice.
Bioassay example 4 control of bacterial basal rot of Rice
The control test of the bacterial basal rot of rice is carried out in the late rice field in Hubei, the number of the control test fields is three, each is 20 square meters, each medicament test field is three, each is 20 square meters, the medicament application time is spray application in the tillering stage, the medicament application concentration is shown in the following table 5, and the disease index of the bacterial basal rot is counted during the medicament application. In order to realize the application concentration, before application, the medicine Y01 is diluted 2500 times, the medicine Y02 is diluted 3000 times, the medicine Y03 is diluted 4400 times, and the medicine Y06, the medicine Y10, the medicine Y14 and the medicine Y18 are respectively diluted 1000 times. Spraying the medicine after dilution, wherein the medicine application frequency is once, and the disease index of the bacterial basal rot is counted by investigation at the heading stage. Disease grading, disease index statistics and prevention and control effect statistics are carried out according to a conventional grading method and a statistical method of the bacterial basal rot of rice.
TABLE 5 prevention and treatment effects on bacterial basal rot of rice
| Medicament | Active ingredient | Application concentration (ppm) | Controlling effect | Theoretical prevention and treatment effect |
| Medicament Y01 | Chitosan oligosaccharide | 20 | 18.6 | -- |
| Medicament Y02 | Hymexazol | 100 | 49.1 | -- |
| Medicament Y03 | Ethylicin | 180 | 62.8 | -- |
| Medicament Y06 | Chitosan oligosaccharide and hymexazol | 20+100 | 61.5 | 58.6 |
| Medicament Y10 | Hymexazol + ethylicin | 100+180 | 83.3 | 81.1 |
| Medicament Y14 | Chitosan oligosaccharide + ethylicin | 20+180 | 71.0 | 69.7 |
| Medicament Y18 | Chitosan oligosaccharide, hymexazol and ethylicin | 20+100+180 | 95.2 | 86.4 |
From the results in the table 5, the ternary complex formulation of chitosan oligosaccharide, hymexazol and ethylicin is synergistic in preventing and treating bacterial basal rot, and can effectively prevent and treat the bacterial basal rot of rice.
Bioassay example 5 control of other diseases
In the rice seed soaking test and the rice root irrigation test in a field, the medicament Y15-Y18 is applied, and the effect of preventing and treating bacterial diseases and fungal diseases can be found.
The above examples are not intended to limit the present invention, and the tests for other plant diseases including the application of different application methods are all technical solutions that can be popularized and verified to have effects by the above embodiments.
Claims (4)
1. A pesticide composition containing chitosan oligosaccharide, hymexazol and ethylicin is characterized by comprising the chitosan oligosaccharide, the hymexazol and the ethylicin, wherein the weight ratio of the chitosan oligosaccharide to the hymexazol to the ethylicin is 1-5:5-15: 10-20.
2. The pesticidal composition according to claim 1, characterized in that the weight ratio of chitosan oligosaccharide, hymexazol and ethylicin is 1-5:7-15: 10-18.
3. The pesticidal composition according to claim 1, characterized in that the sum of the weights of the chitosan oligosaccharide, hymexazol and ethylicin is 2-80% by weight of the pesticidal composition.
4. The pesticidal composition according to claim 1, characterized in that the pesticidal composition is a pesticidal formulation in the form of one of granules, microcapsules, an aqueous emulsion, a suspension, water dispersible granules, a wettable powder and a microemulsion.
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Denomination of invention: A composition containing amino oligosaccharides, chloramphenicol, and allicin and its use Effective date of registration: 20231020 Granted publication date: 20220208 Pledgee: Industrial Bank Co.,Ltd. Haikou Branch Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: Y2023980062064 |
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Address after: Fuchang Village, Laocheng Development Zone, Chengmai County, Hainan Province, 571923 Patentee after: Hainan Zhengye Biotechnology Co.,Ltd. Address before: 571924 Fuchang village, Laocheng Development Zone, Chengmai County, Hainan Province Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. |