CN103719087A - Sterilizing combination containing chunleimeisu and mushroom protein polysaccharide and application thereof - Google Patents
Sterilizing combination containing chunleimeisu and mushroom protein polysaccharide and application thereof Download PDFInfo
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- CN103719087A CN103719087A CN201310700020.5A CN201310700020A CN103719087A CN 103719087 A CN103719087 A CN 103719087A CN 201310700020 A CN201310700020 A CN 201310700020A CN 103719087 A CN103719087 A CN 103719087A
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- kasugarnycin
- mushroom
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- bactericidal composition
- proteoglycan
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Abstract
The invention belongs to the technical field of pesticide, and particularly relates to a compound sterilizing combination containing chunleimeisu and mushroom protein polysaccharide and the application thereof in field of prevention and treatment of crop bacterial and fungus diseases and growth adjusting function. According to the combination, the weight ratio of mushroom protein polysaccharide to chunleimeisu is 1:1 to 1:100, and the combination has the effect of efficacy enhancement through compounding, has a wide application range, low cost, good effects and more obvious treatment effects, and belongs to ideal agent for preventing and treating various bacterial and fungus diseases of crops, such as vegetables, melons, fruits, rice and the like. The combination has strong permeability and moves in plant bodies, is quick to become effective after being sprayed, rainwash resistant and long in lasting period, and can ensure that the color of leaves of melons to which the combination is applied is thick and green and prolong the harvest time. The combination is applied to rice, potatoes, cucumbers, celeries, sorghums, peppers, kidney beans, citruses and fruit trees.
Description
Technical field
The invention belongs to the technical field of agricultural chemicals, be specifically related to a kind ofly contain kasugarnycin and mushroom proteoglycan carries out composite bactericidal composition, and the application aspect control farm crop fungus disease.
Background technology
Kasugarnycin:
(kasugamycin; Kasumin; Kasurabcide; Kasumin-bordeaux; KSM) chemical name 5-amino-2-methyl-6-(2,3,4,5,6-hydroxy-cyclohexyl oxo) tetrahydropyran-3-base) amino-α-IMDA, belong to organic heterocyclic series bactericidal agent.Fusing point (℃): 236～239(decomposes); Hydrochloride: 202～204(decomposes) proterties: sterling is white crystals; Hydrochloride is white needles or flaky crystal, pleasantly sweet.Dissolving situation: sterling is indissoluble in organic solvent is dissolved 12.5%(W/V in 25 ℃ of water); Hydrochloride is soluble in water, is insoluble to the organic solvents such as methyl alcohol, ethanol, acetone, benzene.
Kasugarnycin is the metabolite that actinomycetes produce.In belonging to, inhale antibiotic, have treatment and prevention effect concurrently.
Kasugarnycin is the desirable medicament of preventing and treating various bacteria and fungal disease, has prevention, treatment, growth adjustment function.It is outside the special-purpose antibiotic of control rice blast, also to X. c. pv. oryzicola, and oranges and tangerines gummosis, rubber is sick, Prospect on Kiwifruit Bacterial Canker, pepper bacterial scab is sick, early blight of celery, the confused rot of Kidney bean, the control efficiency that the white helminthosporium leaf spot of water chestnut etc. has had.
This medicine of mushroom proteoglycan (Chitosan Oligosaccharide) is biologic product, is prevention type antivirotic.It is the proteoglycan that the metabolism of edible mushroom thalline produces that virus is played to inhibiting key component, and proteoglycan is pioneering as antivirotic at home.Because preparation includes abundant amino acid, so not only antivirally after dispenser also has obvious production-increasing function.
Mushroom proteoglycan, by passivation virus activity, destroys plant virus gene and virocyte effectively, suppresses virus replication.For preventative antiviral biologic product, the virus disease being caused by TMV, CMV etc. is had to significant control efficiency, should before occurring, virus disease use, and can make crop growth in the phase, not infect virus; And containing materials such as abundant proteoglycan, amino acid and trace elements, and growth and development of plants is had to good facilitation; Nontoxic to people and animals, free from environmental pollution, to plant safety.
Above-mentioned two kinds of medicaments are used separately due to long-term, easily cause the drug-fast generation of germ, cause drug effect obviously to decline, thereby cause dosage greatly to rise, the sprinkling cycle shortens, and result of use is poor, increased cost, the use of a large amount of agricultural chemicals, makes germ resistance more serious, also environment is affected, the medicament residue of agricultural product also increases, have influence on health and the normal existence of the mankind, animal etc., kasugarnycin and mushroom proteoglycan are carried out to composite use control corps diseases, there is not yet report both at home and abroad.
Summary of the invention
The object of the invention is to provide a kind of for the problems referred to above and carry out composite bactericidal composition and application thereof by kasugarnycin and mushroom proteoglycan, said composition plays synergistic effect by composite, applied widely, cost is low, effective, can be used to various bacteria and the fungal disease of the crops such as control control vegetables, melon and fruit and paddy rice.Permeability is strong and can in plant corpus, move, instant effect after spray medicine, and resistance of rainwater washing against, the lasting period is long, and can make melon leaf look dark green after dispenser and extend harvest time.Suitable crop paddy rice, horse official seal potato, cucumber, celery, Chinese sorghum, capsicum, Kidney bean, oranges and tangerines, fruit tree.
In the present invention, the weight of the kasugarnycin of mentioning and the weight of mushroom proteoglycan, active ingredient etc., all refer to the weight after folding hundred, i.e. the weight of active principle.
Bactericidal composition containing kasugarnycin and mushroom proteoglycan of the present invention, its active ingredient consists of kasugarnycin and mushroom proteoglycan, and described kasugarnycin and the weight ratio of mushroom proteoglycan be, few chitose: hymexazo=1:1～1:100.
As preferably, described kasugarnycin and the weight ratio of mushroom proteoglycan be, kasugarnycin and mushroom proteoglycan=1:5～1:20.
Wherein, in said composition, the weight percentage of active ingredient is 1%～99%.
Said composition formulation comprises wetting powder, microemulsion, aqua, adds auxiliary agent and excipient, and the production technology of all knowing according to those skilled in the art can be made into required formulation.
This bactericidal composition is used for preventing and treating that crops are bacillary, function is adjusted in fungal disease and growth, X. c. pv. oryzicola, oranges and tangerines gummosis, rubber is sick, Prospect on Kiwifruit Bacterial Canker, and pepper bacterial scab is sick, early blight of celery, the confused rot of Kidney bean, the control efficiency that the white helminthosporium leaf spot of water chestnut etc. has had, and strengthen the performance of resisting to multiple natural calamities such as low temperature, frost, arid, flooded stain, poisoning, fertilizer damages.
Beneficial effect of the present invention is: kasugarnycin and two kinds of bactericide of mushroom proteoglycan are carried out composite, because the mechanism of action between the two is different, kasugarnycin is the metabolite that actinomycetes produce.In belonging to, inhale antibiotic, have treatment and prevention effect concurrently.It is the proteoglycan that the metabolism of edible mushroom thalline produces that mushroom proteoglycan plays inhibiting key component to virus, and proteoglycan is pioneering as antivirotic at home.Because preparation includes abundant amino acid, so not only antivirally after dispenser also has obvious production-increasing function.The two is composite improves crop and resists various adverse circumstances; and strengthen plant growth gesture; make complex preparation have protection, interior suction conduction function concurrently; expanded fungicidal spectrum, thereby applied widely, composite composition has synergistic effect in certain proportion; not only control efficiency is good; reduce dosage and drug cost, reduced the harmful effect to environment, and greatly delayed the drug-fast generation of germ.In a word, combination sterilization composition of the present invention, has obvious economic benefit and environmental benefit.
The indoor virulence of the composite mixture of having studied kasugarnycin and the different proportionings of mushroom proteoglycan with mycelial growth rate method to cucumber fusarium axysporum, experimental technique adopts the abundant method of Sun Yun to calculate co-toxicity coefficient (CTC) and evaluates mixed effect, CTC value is greater than 120 o'clock mixtures and has synergistic effect, being less than at 80 o'clock is antagonism, has summation action in the time of between 80～120.
Concrete grammar is as follows:
Get the liquid preparing and be placed in 100ml50-60 ℃ of PDA sterilising medium, shake up, pour in the sterilizing culture dish of diameter 60mm, after cooled and solidified, move respectively the bacterium cake that length is consistent, diameter is 4mm of delivering a child, using and add the sterilising medium of equivalent coordinative solvent as blank, all operations all carries out sterile working at superclean bench, and 3 repetitions are established in every processing.Put into 27 ℃ of constant incubators and cultivate after 3-8 days, adopt right-angled intersection method to measure colony diameter, calculate the relevant parameters such as bacteriostasis rate, EC50, CTC, relevant calculating formulas is as follows:
Pure amount of growth=bacterium colony average diameter-bacterium cake diameter (0.4cm)
Bacteriostasis rate=[the pure amount of growth of (contrast pure amount of growth-process pure amount of growth) * 100/ contrast] * 100%
The EC50*100 of the EC50/B agent of toxicity index TI (B)=standard agent A
The EC50*100 of the EC50/AB of actual toxicity index ATI (AB)=A
The percentage of percentage+TI (B) the * B of theoretical toxicity index TTI (AB)=TI (A) * A in mixture in mixture
Co-toxicity coefficient CTC=ATI (AB)/TTI (AB) * 100
Table 1 kasugarnycin and the toxicity test table of mushroom proteoglycan list mixture to cucumber fusarium axysporum
From table 1, kasugarnycin and mushroom proteoglycan are composite, cucumber fusarium axysporum is had to good indoor virulence, the CTC value of seven proportionings is all over 120, show that these two bactericide have good synergistic function under this proportioning, also can find out that in composition, along with kasugarnycin is also different from different its synergistic effects of mushroom proteoglycan ratio, mixed ratio synergy between 1:5～1:20 is particularly remarkable simultaneously.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited thereto, and the formulation preparation method in embodiment is conventional method.
Embodiment 1:4.5% kasugarnycin mushroom derived protein amylase, comprises following component by weight:
By whole mixing of materials, stir and make 4.5% kasugarnycin mushroom derived protein amylase.
Embodiment 2:2.2% kasugarnycin mushroom proteoglycan microemulsion, comprises following component by weight:
The above-mentioned ratio in formula is mixed, obtain 2.2% kasugarnycin mushroom proteoglycan microemulsion.
Embodiment 3:6.3% kasugarnycin mushroom proteoglycan wetting powder, comprises following component by weight:
Said components is carried out to air-flow crushing, make 6.3% kasugarnycin mushroom proteoglycan wetting powder.
Test example: the test of pesticide effectiveness of the mixture control cucumber fusarium axysporum of the different proportionings of kasugarnycin mushroom proteoglycan
3 kinds of mixtures that the embodiment of the present invention is obtained carry out the test of pesticide effectiveness, adopt greenhouse pot culture cucumber seedling, the control efficiency of single mixture of mensuration kasugarnycin mushroom proteoglycan to cucumber fusarium axysporum.When cucumber grows to 3～4 leaves, select the blade of growing way uniformity to adopt manual sprayer to carry out foliar spray processing, after 24 hours, inoculate pathogen, 7d " Invest, Then Investigate " result is cultivated in moisturizing, the results are shown in Table 2.
The control efficiency table of table 2 pair cucumber fusarium axysporum
As can be seen from Table 2, the mixture preparation of kasugarnycin mushroom proteoglycan can effectively be prevented and treated cucumber fusarium axysporum, and is obviously better than the control efficiency of single dose.
Claims (7)
1. containing a bactericidal composition for hymexazo and few chitose, its active ingredient consists of kasugarnycin and mushroom proteoglycan, and described kasugarnycin and the weight ratio of mushroom proteoglycan be, few chitose: hymexazo=1:1～1:100.
2. bactericidal composition according to claim 1, is characterized in that, described kasugarnycin and the weight ratio of mushroom proteoglycan be, kasugarnycin: mushroom proteoglycan=1:5～1:20.
3. bactericidal composition according to claim 1 and 2, is characterized in that, the weight percentage of described active ingredient is 1%～99%.
4. bactericidal composition according to claim 1, is characterized in that, described mushroom proteoglycan main component is mushroom polysaccharide, and its structure is the complex by glucose, mannose, galactose, wood sugar and protein fragments.
5. bactericidal composition according to claim 1, is characterized in that, said composition formulation comprises wetting powder, microemulsion, aqua.
6. bactericidal composition as claimed in claim 1, for preventing and treating the bacillary and fungal disease on crop.
7. bactericidal composition as claimed in claim 1, for preventing and treating controlling object, the rice blast on paddy rice is had to excellent preventive effect, can prevent and treat the raw tail spore of beet on beet, carrot soft gull Wen bacterium on potato, the Kidney bean pseudomonas that dwells on Kidney bean, the pseudomonas that sheds tears on cucumber, as cladosporium leaf and fruit mould of tomato, rice blast, cucumber bacterial angular leaf spot etc.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310700020.5A CN103719087A (en) | 2013-12-18 | 2013-12-18 | Sterilizing combination containing chunleimeisu and mushroom protein polysaccharide and application thereof |
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| CN201310700020.5A CN103719087A (en) | 2013-12-18 | 2013-12-18 | Sterilizing combination containing chunleimeisu and mushroom protein polysaccharide and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922505A (en) * | 2017-02-24 | 2017-07-07 | 安徽榈源农业科技有限公司 | A kind of implantation methods for improving pumpkin bacteriostatic activity |
| CN107535529A (en) * | 2017-07-27 | 2018-01-05 | 张银花 | A kind of agriculturally useful compositions for preventing and treating bacterial leaf streak of rice |
| CN110074113A (en) * | 2019-04-28 | 2019-08-02 | 杭州市农业科学研究院 | Application, plant immune inducer and the application of Stropharia rugoso-annulata polysaccharide |
| CN115152831A (en) * | 2022-07-12 | 2022-10-11 | 湖南应用技术学院 | Cold-chain logistics ecological preservation method for vegetables |
-
2013
- 2013-12-18 CN CN201310700020.5A patent/CN103719087A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922505A (en) * | 2017-02-24 | 2017-07-07 | 安徽榈源农业科技有限公司 | A kind of implantation methods for improving pumpkin bacteriostatic activity |
| CN107535529A (en) * | 2017-07-27 | 2018-01-05 | 张银花 | A kind of agriculturally useful compositions for preventing and treating bacterial leaf streak of rice |
| CN110074113A (en) * | 2019-04-28 | 2019-08-02 | 杭州市农业科学研究院 | Application, plant immune inducer and the application of Stropharia rugoso-annulata polysaccharide |
| CN110074113B (en) * | 2019-04-28 | 2021-07-13 | 杭州市农业科学研究院 | Application of stropharia rugoso-annulata polysaccharide, plant immunity inducer and application |
| CN115152831A (en) * | 2022-07-12 | 2022-10-11 | 湖南应用技术学院 | Cold-chain logistics ecological preservation method for vegetables |
| CN115152831B (en) * | 2022-07-12 | 2023-09-05 | 湖南应用技术学院 | Vegetable cold-chain logistics ecological preservation method |
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Application publication date: 20140416 |