CN103651421A - Bactericidal composition containing tetramycin and bismerthiazol and application of bactericidal composition - Google Patents
Bactericidal composition containing tetramycin and bismerthiazol and application of bactericidal composition Download PDFInfo
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- CN103651421A CN103651421A CN201210334797.XA CN201210334797A CN103651421A CN 103651421 A CN103651421 A CN 103651421A CN 201210334797 A CN201210334797 A CN 201210334797A CN 103651421 A CN103651421 A CN 103651421A
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- tetramycin
- yekuzuo
- bactericidal composition
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Abstract
The invention belongs to the technical field of pesticides and specifically relates to compounded bactericidal composition missible oil containing tetramycin and bismerthiazol and application of the bactericidal composition in preventing rice bacterial leaf blight. In the composition, a weight ratio of the tetramycin to the bismerthiazol is 1:5-1:200; synergism is achieved by compounding; a charging sequence, operation, and the like are optimized by adopting an emulsion in water and a preparation method thereof, so that the original preparation technology is improved. Besides, the bactericidal composition has good prevention and treatment effect for rice bacterial leaf blight, and has a good effect in delaying drug resistance of pathogenic bacteria.
Description
Technical field
The invention belongs to the technical field of agricultural chemicals, be specifically related to a kind ofly contain tetramycin and Yekuzuo carries out composite bactericidal composition, and the application aspect bacterial blight of rice.
Background technology
Tetramycin has another name called tetramycin, 11371 antibiotic, derive from strain actinomycetes that separate in the soil sample of Wuzhou, Guangxi, streptomyces hygroscopicus Wuzhou subspecies (Streptomyces ahygroscopicn wuzhouensisn subsp.), do not belong to actinomycetes, streptomyces.The metabolite producing in conk process, generally by through engineering approaches fermenting and producing.This product fungicidal spectrum is wide, and three main kind of 26 kinds of known disease funguses such as mastigomycetes, sac fungi and Deuteromycotina fungi are all had to extremely strong killing action.The control of the applicable multiple fungal disease of various crops.Especially to fruit tree putrefaction disease, spot defoliation; Cotton yellow (withered) disease of withering; Root rot; Bacterial blight of rice and banded sclerotial blight, seedling blight; Ginseng and pseudo-ginseng black spot; Tealeaves tea gall; Fruit white rot of grape, the climing rot of watermelon, root rot, the rotten special efficacy of stem foot.Can obviously promote callus healing, promote weak seedling well developed root system, the recovery of aging root system, improve crop disease-resistant ability and optimize crop quality simultaneously.
Yekuzuo (bismerthlazol) is the interior suction series bactericidal agent of the autonomous initiative of China.Yekuzuo (bismerthlazol) does not have teratogenesis toxicity through animal experiment proof, and obtains national medicine inspection department and the registration of agricultural chemicals department.Novel germicide " Yekuzuo (bismerthlazol) " is the thiazoles new medicament of control bacterial disease, high-efficiency low-toxicity.1979 Nian Huo Zhejiang Province science conference second prizes.Nineteen eighty-two-1984 year are by joint study successes such as industry science Suo He Wenzhou City, the Wenzhou City, Zhejiang Province institutes of agricultural sciences.In land for growing field crops, use, obtain better control efficiency.Within 1984, go into operation, obtain Zhejiang Province's scientific and technological achievement second prize.The international pioneering new type bactericide agricultural chemicals of Ta Shi, efficient, low toxicity, low-residual, the lasting period is long, interior absorption is strong, to preventing and treating bacterial blight of rice, bacterial leaf spot, citrus ulcer etc., have good control efficiency.
Above-mentioned two kinds of medicaments are used separately due to long-term, easily cause the drug-fast generation of germ, cause drug effect obviously to decline, thereby cause dosage greatly to rise, the sprinkling cycle shortens, and result of use is poor, increased cost, the use of a large amount of agricultural chemicals, makes germ resistance more serious, also environment is affected, the medicament residue of agricultural product also increases, have influence on health and the normal existence of the mankind, animal etc., tetramycin and Yekuzuo are carried out to composite use control corps diseases, there is not yet report both at home and abroad.
Summary of the invention
The object of the invention is to provide a kind of for the problems referred to above and carry out composite bactericidal composition and application thereof by tetramycin and Yekuzuo, said composition plays synergistic effect by composite, bacterial blight of rice is had to good control efficiency, to delaying the pesticide resistance of pathogen, have good effect simultaneously.
In the present invention, the weight of the tetramycin of mentioning and the weight of Yekuzuo, active ingredient etc., all refer to the weight after folding hundred, i.e. the weight of active principle.
Bactericidal composition containing tetramycin and Yekuzuo of the present invention, its active ingredient consists of tetramycin and Yekuzuo, and described tetramycin and the weight ratio of Yekuzuo be, tetramycin: Yekuzuo=1:5～1:200.
As preferably, described tetramycin and the weight ratio of Yekuzuo be, tetramycin: Yekuzuo=1:10～1:100.
Wherein, in said composition, the weight percentage of active ingredient is 10.1%～22%.
This combination is the aqueous emulsion of bactericidal composition, comprises following component by weight: tetramycin 0.1-2%, and Yekuzuo 10-20%, emulsifier 5-15%, cosolvent 5-20%, antifreezing agent 3-7%, defoamer 0.5-3%, thickener 0-2.5%, surplus is water.
Described cosolvent is one or both in toluene, dimethylbenzene, solvent naphtha, cyclohexanone, dimethyl formamide, ethyl acetate, acetone or vegetable oil.
Described non-ionic surface active agent is one or more in phenethyl phenol polyethenoxy ether, phenethyl phenol polyethenoxy polyethenoxy ether, alkylphenol polyoxyethylene, alkylphenol polyoxyethylene formaldehyde condensation products, fatty alcohol-polyoxyethylene ether, castor oil polyoxyethylene ether, polystyrene-based phenol formaldehyde resin APEO, phenethyl phenol polyethenoxy ether phosphate or alkylphenol polyoxyethylene phosphate;
Described anion surfactant is one or more in alkyl benzene calcium sulfonate, sodium alkyl benzene sulfonate or C8-C20 sodium alkyl sulfate.
Described antifreezing agent is one or more in ethylene glycol, propane diols, glycerine, polyethylene glycol, sorbierite or urea.
Described defoamer is organic silicone oil or isooctanol.
Described thickener is one or more in xanthans, gum Arabic, carboxymethyl cellulose, CMC or polyvinyl alcohol.
This bactericidal composition is used for preventing and treating bacterial blight of rice.
Beneficial effect of the present invention is: tetramycin and two kinds of bactericide of Yekuzuo are carried out composite, because the mechanism of action between the two is different, tetramycin has interior suction bacteriostatic activity, stop germ to be invaded and expansion, Yekuzuo is efficient, low toxicity, low-residual, lasting period is long, interior absorption is strong, to preventing and treating bacterial blight of rice, bacterial leaf spot, citrus ulcers etc. have good control efficiency, composite composition has synergistic effect in certain proportion, not only control efficiency is good, dosage and drug cost have been reduced, reduced the harmful effect to environment, and greatly delayed the drug-fast generation of germ.This bactericidal composition, can be used to prevent and treat bacterial blight of rice, and can doublely control bacterial disease as cucumber bacterial angular leaf spot etc., there is good control efficiency, to delaying the pesticide resistance of pathogen, there is good effect simultaneously, in a word, combination sterilization composition of the present invention, has obvious economic benefit and environmental benefit.
The indoor virulence of the composite mixture of having studied tetramycin and the different proportionings of Yekuzuo with mycelial growth rate method to bacterial blight of rice germ, experimental technique adopts the abundant method of Sun Yun to calculate co-toxicity coefficient (CTC) and evaluates mixed effect, CTC value is greater than 120 o'clock mixtures and has synergistic effect, being less than at 80 o'clock is antagonism, has summation action in the time of between 80～120.
Concrete grammar is as follows:
Get the liquid preparing and be placed in 100ml 50-60 ℃ of PDA sterilising medium, shake up, pour in the sterilizing culture dish of diameter 60 mm, after cooled and solidified, move respectively the bacterium cake that length is consistent, diameter is 4mm of delivering a child, using and add the sterilising medium of equivalent coordinative solvent as blank, all operations all carries out sterile working at superclean bench, and 3 repetitions are established in every processing.Put into 27 ℃ of constant incubators and cultivate after 3-8 days, adopt right-angled intersection method to measure colony diameter, calculate bacteriostasis rate, EC
50, the relevant parameter such as CTC, relevant calculating formulas is as follows:
Pure amount of growth=bacterium colony average diameter-bacterium cake diameter (0.4cm)
Bacteriostasis rate=[the pure amount of growth of (contrast pure amount of growth-process pure amount of growth) * 100/ contrast] * 100%
The EC50*100 of the EC50/B agent of toxicity index TI(B)=standard agent A
The EC50*100 of the EC50/AB of actual toxicity index ATI (AB)=A
The percentage of percentage+TI (B) the * B of theoretical toxicity index TTI (AB)=TI (A) * A in mixture in mixture
Co-toxicity coefficient CTC=ATI(AB)/TTI(AB) * 100
Table 1 tetramycin and the toxicity test table of Yekuzuo list mixture to bacterial blight of rice germ
| Reagent agent | Active ingredient weight ratio | EC50（mg/L） | Co-toxicity coefficient (CTC) |
| Tetramycin | —— | 2 | —— |
| Yekuzuo | —— | 200 | —— |
| Tetramycin: Yekuzuo | 1：5 | 60 | 122.55 |
| Tetramycin: Yekuzuo | 1：10 | 220 | 240.55 |
| Tetramycin: Yekuzuo | 1：100 | 202 | 250.88 |
| Tetramycin: Yekuzuo | 1：200 | 201 | 188.94 |
From table 1, tetramycin and Yekuzuo are composite, bacterial blight of rice germ is had to good indoor virulence, the CTC value of five proportionings is all over 120, show that these two bactericide have good synergistic function under this proportioning, also can find out that its synergistic effect of difference along with tetramycin and Yekuzuo compound proportion is also different in composition, mixed ratio synergy between 1:10～1:100 is particularly remarkable simultaneously.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited thereto, and the formulation preparation method in embodiment is conventional method.
Embodiment 1:10.1% tetramycin Yekuzuo aqueous emulsion, comprises following component by weight:
Tetramycin 0.1%
Yekuzuo 10%
Alkyl naphthalene formaldehyde condensate sulfonates 3%
Polyoxyethylene nonylphenol ether 3%
Xanthans 0.3%
Benzoic acid 0.1%
Organosilan defoamer 0.5%
Water surplus
By whole mixing of materials, after stirring, material is joined to sand mill and carry out sand milling, make material reach 2～5 μ m fineness, make 10.1% tetramycin Yekuzuo aqueous emulsion.
Embodiment 2:22% tetramycin Yekuzuo aqueous emulsion, comprises following component by weight:
Tetramycin 2%
Yekuzuo 20%
Alkyl naphthalene formaldehyde condensate sulfonates 3%
Polyoxyethylene nonylphenol ether 4%
Xanthans 0.3%
Benzoic acid 0.1%
Organosilan defoamer 0.5%
Water surplus
By whole mixing of materials, after stirring, material is joined to sand mill and carry out sand milling, make material reach 2～5 μ m fineness, make 22% tetramycin Yekuzuo aqueous emulsion.
Test example: tetramycin and the mixture of the different proportionings of Yekuzuo are prevented and treated the test of pesticide effectiveness of bacterial blight of rice
2 kinds of mixtures that the embodiment of the present invention is obtained carry out the test of pesticide effectiveness, adopt greenhouse pot culture paddy rice, the control efficiency of single mixture of mensuration tetramycin and Yekuzuo to bacterial blight of rice.When paddy rice one leaf is wholeheartedly time, select the blade of growing way uniformity to adopt manual sprayer to carry out foliar spray processing, after 24 hours, inoculate pathogen, 7d " Invest, Then Investigate " result is cultivated in moisturizing, the results are shown in Table 2.
The control efficiency table of table 2 pair bacterial blight of rice
| Reagent agent | Dosage (μ g/mL) | Disease index | Control efficiency (%) |
| Embodiment 1 product | 200 | 12.44 | 82.77 |
| Embodiment 2 products | 200 | 15.76 | 78.17 |
| 20% tetramycin aqueous emulsion | —— | —— | —— |
| 20% Yekuzuo aqueous emulsion | 200 | 18.76 | 74.02 |
| Clear water contrast | —— | 72.21 | —— |
As can be seen from Table 2, the mixture preparation of tetramycin and Yekuzuo can effectively be prevented and treated bacterial blight of rice, and is obviously better than the control efficiency of single dose.
Claims (9)
1. containing a bactericidal composition aqueous emulsion for tetramycin and Yekuzuo, its active ingredient consists of tetramycin and Yekuzuo, and described tetramycin and the weight ratio of Yekuzuo be, tetramycin: Yekuzuo=1:5～1:200.
2. bactericidal composition according to claim 1, is characterized in that, described tetramycin and the weight ratio of Yekuzuo be, tetramycin: Yekuzuo=1:10～1:100.
3. bactericidal composition according to claim 1 and 2, is characterized in that, the weight percentage of described active ingredient is 10.1%～22%.
4. the aqueous emulsion of the bactericidal composition as described in right 1, comprises following component by weight: tetramycin 0.1-2%, and Yekuzuo 10-20%, emulsifier 5-15%, cosolvent 5-20%, antifreezing agent 3-7%, defoamer 0.5-3%, thickener 0-2.5%, surplus is water.
5. aqueous emulsion according to claim 4, is characterized in that, described cosolvent is one or both in toluene, dimethylbenzene, solvent naphtha, cyclohexanone, dimethyl formamide, ethyl acetate, acetone or vegetable oil.
6. aqueous emulsion according to claim 4, it is characterized in that, described non-ionic surface active agent is one or more in phenethyl phenol polyethenoxy ether, phenethyl phenol polyethenoxy polyethenoxy ether, alkylphenol polyoxyethylene, alkylphenol polyoxyethylene formaldehyde condensation products, fatty alcohol-polyoxyethylene ether, castor oil polyoxyethylene ether, polystyrene-based phenol formaldehyde resin APEO, phenethyl phenol polyethenoxy ether phosphate or alkylphenol polyoxyethylene phosphate; Described anion surfactant is one or more in alkyl benzene calcium sulfonate, sodium alkyl benzene sulfonate or C8-C20 sodium alkyl sulfate.
7. aqueous emulsion according to claim 4, is characterized in that, described antifreezing agent is one or more in ethylene glycol, propane diols, glycerine, polyethylene glycol, sorbierite or urea.
8. aqueous emulsion according to claim 4, is characterized in that, described defoamer is organic silicone oil or isooctanol.
9. aqueous emulsion according to claim 4, is characterized in that, described thickener is one or more in xanthans, gum Arabic, carboxymethyl cellulose, CMC or polyvinyl alcohol.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210334797.XA CN103651421A (en) | 2012-09-12 | 2012-09-12 | Bactericidal composition containing tetramycin and bismerthiazol and application of bactericidal composition |
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| CN201210334797.XA CN103651421A (en) | 2012-09-12 | 2012-09-12 | Bactericidal composition containing tetramycin and bismerthiazol and application of bactericidal composition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104770376A (en) * | 2015-04-24 | 2015-07-15 | 浙江世佳科技有限公司 | Tetramycin and azoxystrobin containing sterilizing aqueous emulsion and preparation method thereof |
| CN104770379A (en) * | 2015-04-24 | 2015-07-15 | 浙江世佳科技有限公司 | Tetramycin and difenoconazole containing sterilizing aqueous emulsion and preparation method thereof |
| CN107318855A (en) * | 2017-06-04 | 2017-11-07 | 中国农业科学院植物保护研究所 | Prevent and treat the composition of agricultural disease |
-
2012
- 2012-09-12 CN CN201210334797.XA patent/CN103651421A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104770376A (en) * | 2015-04-24 | 2015-07-15 | 浙江世佳科技有限公司 | Tetramycin and azoxystrobin containing sterilizing aqueous emulsion and preparation method thereof |
| CN104770379A (en) * | 2015-04-24 | 2015-07-15 | 浙江世佳科技有限公司 | Tetramycin and difenoconazole containing sterilizing aqueous emulsion and preparation method thereof |
| CN107318855A (en) * | 2017-06-04 | 2017-11-07 | 中国农业科学院植物保护研究所 | Prevent and treat the composition of agricultural disease |
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Application publication date: 20140326 |