CN113331191A - Biological control technology for citrus canker - Google Patents
Biological control technology for citrus canker Download PDFInfo
- Publication number
- CN113331191A CN113331191A CN202110525313.9A CN202110525313A CN113331191A CN 113331191 A CN113331191 A CN 113331191A CN 202110525313 A CN202110525313 A CN 202110525313A CN 113331191 A CN113331191 A CN 113331191A
- Authority
- CN
- China
- Prior art keywords
- citrus canker
- biological control
- wettable powder
- amino
- oligosaccharin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a biological control technology for citrus canker, which is characterized by being prepared by uniformly mixing 79-89% of oxytetracycline hydrochloride, 2-15% of amino-oligosaccharin and 1-12% of allicin in by weight percentage. Preferably 88% of oxytetracycline hydrochloride, 7% of amino-oligosaccharin and 5% of allicin. The wettable powder for biological control of citrus canker is applied to preparation of wettable powder for biological control of citrus canker. The invention can effectively enhance the prevention and treatment effects of citrus canker, has quick response and long duration in the initial stage of disease, and has no phytotoxicity influence on target and non-target crops; belongs to biological control, has no residue, is green and safe, is beneficial to environmental protection and the like.
Description
Technical Field
The invention relates to the field of biological control of citrus canker.
Background
1. Citrus canker is a disease in the world and is also one of the important diseases in citrus producing areas. Is caused by Xanthomonas carpi and occurs in citrus diseases. Leaves, branches and tips, fruits and sepals are mainly damaged, when serious diseases occur, fallen leaves, fallen fruits and branches and tips die, normal growth of plants is influenced, and when fruits are infected, the fruits are rotten and lose commodity value. At present, chemical control, single biological control, chemical biological mixed control and other control technologies are mostly adopted in the control technology for control.
2. Although the prevention and control have certain prevention and control effects, the prevention and control have the defects of environmental pollution, safety residue on agricultural products and food, easy generation of drug resistance, increased toxicity, influence by climate and temperature, slow and unobvious effect, influence on disease resistance of plants and the like. For the prevention and treatment of citrus canker, the requirements of green safety, difficult generation of drug resistance, obvious effect, small influence by climate and temperature, enhancement of plant disease resistance and improvement of agricultural product quality are required.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provide a biological control technology for citrus canker.
The invention aims to realize that the wettable powder for biologically preventing and controlling citrus canker is characterized by being prepared from 79-89% of oxytetracycline hydrochloride, 2-15% of amino-oligosaccharin and 1-12% of allicin by weight percent through uniformly mixing.
The wettable powder for biological control of citrus canker is characterized by comprising the following components in parts by weight: 88% of oxytetracycline hydrochloride, 7% of amino-oligosaccharin and 5% of allicin.
The wettable powder for biological control of citrus canker is applied to preparation of wettable powder for biological control of citrus canker.
The invention has the characteristics and advantages that:
1) the invention relates to a biological agent for preventing and treating plant bacterial diseases and compounding and application thereof. In particular to a compound preparation and an application for preventing and treating citrus canker, the dosage form is wettable powder, and the pesticide belongs to biological pesticides.
2) The oxytetracycline hydrochloride is mainly effective to plant bacteria, bacteroides, rickettsia and other diseases, and has strong inhibiting effect on bacterial protein synthesis. Can be absorbed by plant leaves and conducted to other tissues.
3) The amino-oligosaccharin can induce and activate the immune system of plants, enhance disease resistance and improve the antiviral ability of plants. Can inhibit the expression of virus gene, control virus propagation, repair the damaged plant, promote root growth, strengthen crop resistance and promote plant growth.
4) The allicin sterilization mechanism can penetrate through cell membranes of bacterial diseases to enter cytoplasm, and competitively inhibits compounds containing sulfydryl to inactivate the compounds, so that bacteria cannot carry out biological oxidation, metabolism is damaged, and the effect of inhibiting growth and reproduction of germs is achieved.
5) According to the invention, the optimized formula of oxytetracycline hydrochloride 88%, amino-oligosaccharide 0.5% and allicin 0.3% compounded wettable powder can synergistically exert respective bactericidal effects and delay the generation of drug resistance; the effects of preventing and treating citrus canker are effectively enhanced, the effect is quick in application in the initial stage of disease occurrence, the effect duration is long, and no phytotoxicity influence is caused on target and non-target crops; belongs to biological control, has no residue, is green and safe, is beneficial to environmental protection and the like.
Detailed Description
1) The components of the invention are 88 percent of oxytetracycline hydrochloride, 7 percent of amino-oligosaccharin and 5 percent of allicin; the preparation method comprises the following steps: 88 g of oxytetracycline hydrochloride, 7 g of amino-oligosaccharin and 5 g of allicin are mixed uniformly and then compounded into wettable powder.
2) The invention has the beneficial effect that the single-use medicine of citrus canker is compounded into wettable powder.
3) The invention has the effect of controlling citrus canker
(1) Design of experiments
The experiment was set to 5 treatments: 1) oxytetracycline hydrochloride 88% + 12% of clear water, 2) amino-oligosaccharin 7% + 93% of clear water, 3) allicin 5% + 95% of clear water; 4) 88% of oxytetracycline hydrochloride, 7% of amino-oligosaccharin and 5% of allicin; 5) and (3) 100% clear water Control (CK), spraying the navel oranges in the navel orange planting base after diluting the 5 formulas with clear water according to the mass ratio of 1:3500 of each formula and the clear water, repeating the treatment for 3 times, and designing random block groups, wherein the area of a cell is 333.33 square meters.
(2) Test method
The test site is in the navel orange planting base of Yuexicun mountain pool in xi tan town of Fuan city, Ning de City, Fujian province, the tested crop is the New Youer navel orange, the medicine is sprayed at the initial stage of the onset of the citrus canker disease, the medicine is sprayed once every 20 days and is continuously sprayed for 3 times, and the control effect is investigated after the last medicine is sprayed for 30 days.
(3) Investigation method
Sampling 5 points in each cell by adopting a five-point method, investigating 1 plant at each point, randomly selecting 5 leaves on the upper part, the lower part, the left part, the middle part, the right part and the back part of each plant, investigating the number of diseased leaves in each treatment cell, and calculating the diseased leaf rate, the disease index and the prevention and treatment effect. Diseased leaf rate (%) = diseased leaf number/total leaf number investigated × 100; disease index = (number of diseased leaves at each stage × disease grade value)/(number of investigated total leaves × highest grade value) × 100; control effect (%) = (blank control disease index-disease index after drug treatment area)/blank control disease index x 100
Citrus canker grading criteria were as follows: level 0: no disease; level 1: the area of the lesion spots accounts for less than 5% of the area of the whole leaf; and 3, level: the area of the lesion spots accounts for 6 to 25 percent of the area of the whole leaf; and 5, stage: the area of the lesion spots accounts for 26-50% of the area of the whole leaf; and 7, stage: the area of the lesion spots accounts for more than 51 percent of the area of the whole leaf.
TABLE 1 controlling effect of wettable powder of the present invention on citrus canker
(1) Test results show that the control effect of the wettable powder compounded by the oxytetracycline hydrochloride, the amino-oligosaccharin and the allicin on citrus canker is shown in table 1, and the control effect is 99.33% and is obvious from the table 1.
(2) Investigation of phytotoxicity
The visual inspection is carried out for 19 consecutive days after the first pesticide application, the visual inspection is carried out for 19 consecutive days after the second pesticide application, and the visual inspection is carried out for 30 consecutive days after the third pesticide application, so that no phytotoxicity influence is caused on the target crops and the non-target crops.
Claims (3)
1. A wettable powder for preventing and treating the ulcer of orange is prepared from oxytetracycline hydrochloride (79-89 wt.%), amino-oligosaccharin (2-15) and garlicin (1-12) through proportional mixing.
2. Wettable powder for the biological control of citrus canker diseases according to claim 1, characterized in that: 88% of oxytetracycline hydrochloride, 7% of amino-oligosaccharin and 5% of allicin.
3. Use of the wettable powder for biocontrol of citrus canker disease as claimed in any one of claims 1-2 for the preparation of wettable powder for biocontrol of citrus canker disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110525313.9A CN113331191A (en) | 2021-05-14 | 2021-05-14 | Biological control technology for citrus canker |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110525313.9A CN113331191A (en) | 2021-05-14 | 2021-05-14 | Biological control technology for citrus canker |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113331191A true CN113331191A (en) | 2021-09-03 |
Family
ID=77469774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110525313.9A Pending CN113331191A (en) | 2021-05-14 | 2021-05-14 | Biological control technology for citrus canker |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113331191A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114793716A (en) * | 2022-04-21 | 2022-07-29 | 福建省农业科学院果树研究所 | Method for preventing and treating chronic senescence of citrus |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884301A (en) * | 2010-04-08 | 2010-11-17 | 浙江省农业科学院 | Method for preventing pollution in plant tissue culture |
| CN101904338A (en) * | 2010-07-28 | 2010-12-08 | 江西农业大学 | Compound drug for preventing and curing citrus canker |
| CN102365945A (en) * | 2011-10-28 | 2012-03-07 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composition for resisting citrus canker disease as well as application and method thereof |
| CN110150278A (en) * | 2019-07-02 | 2019-08-23 | 山东亿嘉农化有限公司 | A kind of bactericidal composition containing copper acetate and amino-oligosaccharide and its application in citrus bacterial canker disease |
| CN111084188A (en) * | 2019-12-30 | 2020-05-01 | 海南正业中农高科股份有限公司 | Composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof |
-
2021
- 2021-05-14 CN CN202110525313.9A patent/CN113331191A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101884301A (en) * | 2010-04-08 | 2010-11-17 | 浙江省农业科学院 | Method for preventing pollution in plant tissue culture |
| CN101904338A (en) * | 2010-07-28 | 2010-12-08 | 江西农业大学 | Compound drug for preventing and curing citrus canker |
| CN102365945A (en) * | 2011-10-28 | 2012-03-07 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composition for resisting citrus canker disease as well as application and method thereof |
| CN110150278A (en) * | 2019-07-02 | 2019-08-23 | 山东亿嘉农化有限公司 | A kind of bactericidal composition containing copper acetate and amino-oligosaccharide and its application in citrus bacterial canker disease |
| CN111084188A (en) * | 2019-12-30 | 2020-05-01 | 海南正业中农高科股份有限公司 | Composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof |
Non-Patent Citations (6)
| Title |
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| 刘子龙: "农用抗生素在水果生产上的应用", 《果农之友》 * |
| 吴洪生等: "中药材常用生物农药种类及特性(下)", 《中药研究与信息》 * |
| 孙惠敏等: "几种杀菌剂对柑桔溃疡病的生物活性", 《江西农业大学学报》 * |
| 左经龙等: "细菌性病害主要化学防治药剂", 《吉林蔬菜》 * |
| 彭昌家等: "四川南充柑橘病虫害绿色防控技术", 《中国果树》 * |
| 李文娟: "砂糖桔保花保果技术要点", 《广西园艺》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114793716A (en) * | 2022-04-21 | 2022-07-29 | 福建省农业科学院果树研究所 | Method for preventing and treating chronic senescence of citrus |
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| RJ01 | Rejection of invention patent application after publication | ||
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Application publication date: 20210903 |