CN109874791A - A kind of lactic acid thimerosal and application thereof - Google Patents
A kind of lactic acid thimerosal and application thereof Download PDFInfo
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- CN109874791A CN109874791A CN201910133692.XA CN201910133692A CN109874791A CN 109874791 A CN109874791 A CN 109874791A CN 201910133692 A CN201910133692 A CN 201910133692A CN 109874791 A CN109874791 A CN 109874791A
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- lactic acid
- thimerosal
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- silkworm
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Abstract
The invention discloses a kind of lactic acid thimerosals, which is characterized in that the thimerosal by weight, including following component part: lactic acid content: 8.1wt%-12.3wt%；Neopelex 70.1wt%-90.1wt%.The invention also discloses the purposes of the lactic acid thimerosal.The present invention provides a kind of low toxicity for the inactivation of nosema bombycis, and inactivation ratio is high, the short lactic acid thimerosal of disinfecting time.Compared with existing inactivation product, the advantage of this product further include: disinfection way is simple, the natural noresidue of the product of use.
Description
Technical field
The present invention relates to thimerosal fields, and in particular to a kind of lactic acid thimerosal and application thereof.
Background technique
Nosema bombycis, scientific name Nosema bombycis are under the jurisdiction of mycota (Fungi), Microspora
(Microspora), Microsporidea (Microsporea), microsporidian mesh (Microsporida) are a kind of intracellular special
Property parasitic eucaryote.Carl Naggli isolated nosema bombycis in silkworm for the first time in 1857, can according to it
It is parasitic in silkworm, it is named as " nosema bombycis " then.
Nosema bombycis is that Naggli was separated in silkworm for the first time in 1857, can be posted in silkworm according to it
It is raw, it is named as " nosema bombycis " (Nosema bombycis) then.Since nosema bombycis can both eat lower infection
It can be infected again with embryo, fatal damage is caused to sericultural production, therefore micro- armful of sub- worm of silkworm is decided to be only on sericultural production
One legal quarantine object, and become the key points and difficulties of sericulture circle research.1845, the French continent Vaucluse the first explosion man
Granulosis is grasped, other European silkworm areas is then spread to rapidly, brings destruction to the European sericulture of the 17th century；Then particulate
Disease travels to Asian countries again, and such as Japan and China cause the loss of up to ten million members.
Therefore it is particularly important to study a kind of thimerosal that can effectively kill nosema bombycis.
Summary of the invention
In order to overcome drawbacks described above of the existing technology, one of the objects of the present invention is to provide a kind of disinfections of lactic acid
Liquid.
The second object of the present invention is the purposes of the lactic acid thimerosal.
One of in order to achieve the object of the present invention, used technical solution is:
A kind of lactic acid thimerosal, calculates by weight, including following component part:
Lactic acid content: 8.1wt%-12.3wt%；；
Neopelex 70.1wt%-90.1wt%.
In a preferred embodiment of the invention, the lactic acid includes L lactic acid or DL lactic acid.
In order to achieve the object of the present invention two, used technical solution is:
A kind of purposes of lactic acid thimerosal, wherein the purposes is the deactivation to nosema bombycis.
In a preferred embodiment of the invention, the deactivation is not surpass lactic acid thimerosal dilution
500 times are crossed, and carries out disinfection treatment not less than 60 minutes.
In a preferred embodiment of the invention, the deactivation is not surpass lactic acid thimerosal dilution
400 times are crossed, and carries out disinfection treatment not less than 5 minutes.
The beneficial effects of the present invention are: a kind of low toxicity is provided for the inactivation of nosema bombycis, inactivation ratio is high, disinfection
Time short lactic acid thimerosal.Compared with existing inactivation product, the advantage of this product further include: disinfection way is simple, uses
The natural noresidue of product.
Specific embodiment
Steps are as follows for specific embodiment:
(1) silkworm is used in test
1. kind: with the existing common four-moulter kind of bivoltine, silkworm egg used is bright moon × cyanines pine cenospecies.
2. source: qualified silkworm egg (breeding of autumn in 2017, the batch of seed station Co., Ltd, industry production
13)。
3. quantity: each test group sets 3rd area, every area silkworm 25.
(2) trial drug
1. test medicine and source: test medicine " lactic acid thimerosal (silkworm use) " is limited by extra large strategic point taste (Shanghai) biotechnology
Company develop, it is completely the same with the preparation of quasi- listing, 62.5ml/ bottles of packing specification, there is complete target level of product quality, have in accordance with
The specification of prescribed form.
(3) test grouping
It carries out disinfactant for silkworm and tests time-division following each group.
1. group (is not infected and is not handled) in blank control.
2. infection does not handle group (negative control group): disinfectant solution is replaced with sterile water, by the same method of test group
It is tested.
3. test group: using nosema bombycis as sterilization objects, with " lactic acid thimerosal (silkworm use) " various concentration and work
It is tested with the time.
Each group sets 3 repetitions, and each repetition (unit) is with silkworm from 25 2 ages.
(4) test method
1. cause of disease: the nosema bombycis purified using rejuvenation in 2018 is stored in Chinese Academy of Agricultural Sciences's sericulture research
Institute's silkworm physiology and pathological study room, original liquid concentration are 2.8 X 109/ ml, concentration is 2.8 X 10 when effect8/ml。
2. disinfectant minimizing technology: according to should disinfectant characteristic and sterilization objects, this test quantitatively killed using suspension
Test of going out carries out, since the disinfectant is currently without suitable neutralizer, so, disinfection knot is made using the method for centrifugation
Beam.That is: it 1 minute before the disinfecting time for reaching setting, is centrifuged 10 minutes with 3000rpm, outwells thimerosal, be added same amount of
Sterile water, concussion keep microsporidian evenly dispersed.
3. sterilization method: quantifying killing method using suspension and tested.The thimerosal 0.9ml of various concentration is first taken to be added to
In test tube, then being separately added into 0.1ml concentration is 2.8 X 109The microsporidian suspension of/ml, shakes up, and makes the final dense of microsporidian
Degree is 2.8 X 108/ ml is acted on 60,30,15,5 minutes respectively, is centrifuged within 1 minute before the action time for reaching setting,
The sterile water for falling to be added after thimerosal same amount gently cleans, and removes remaining disinfectant, adds 1ml sterile water, and concussion is mixed
After even, the liquid after drawing 40 μ l disinfection with pipettor is added to the round mulberry leaf on piece of diameter 2cm, is fed for 25 after smearing uniformly
Silkworm from 2 ages, 5, every area, each processing repeat 3rd area, change after silkworm eats up the mulberry leaf of the above processing and feed nontoxic new fresh mulberry leaf.Infection is not
Processing group replaces thimerosal with sterile water, handles by ibid method.Raising checks microsporidian sense with microscope in 7 days or so one by one
Situation is contaminated, statistics infection silkworm head number calculates each group disease incidence, inactivation ratio is then calculated as follows:
(5) test result
1, various concentration " lactic acid thimerosal (silkworm use) " is to the killing effect of nosema bombycis
Table 1 is that various concentration " lactic acid thimerosal (silkworm use) " acts on 60 minutes inactivation ratios to microsporidian
Table 1
| Extension rate | Action time (min) | Disease incidence (%) | Inactivation ratio (%) |
| 100 | 60 | 0 | 100 |
| 200 | 60 | 0 | 100 |
| 500 | 60 | 0 | 100 |
| 1000 | 60 | 100 | 0 |
| 2000 | 60 | 100 | 0 |
| 4000 | 60 | 100 | 0 |
| Negative control group | / | 100 | 0 |
| Blank control group | / | 0 | / |
With 100,200,500,1000,2000,4000 times of dilution of " lactic acid thimerosal (silkworm use) " respectively to the micro- spore of silkworm
Sub- worm acts on 60 minutes, and " lactic acid thimerosal (the silkworm use) " for investigating various concentration handles the killing to microsporidian in 60 minutes and makees
With.As a result the dilution such as 1,100,200,500 times of table handles nosema bombycis 60 minutes, has to microsporidian
100% inactivation ratio, and 1000,2000,4000 times of dilution to microsporidian without deactivation.
2, various concentration " lactic acid thimerosal (silkworm use) " acts on the deactivation to nosema bombycis in 30 minutes
In order to further appreciate that " lactic acid thimerosal (the silkworm use) " of various concentration to the killing effect of nosema bombycis, it is right
Deactivation of " lactic acid thimerosal (the silkworm use) " of above same concentrations under the conditions of acting on 30 minutes to microsporidian.As a result such as
Table 2, when acting on 30 minutes, 100,200 times of dilution still has 100% inactivation ratio, and 500 times of inactivation ratio only has 4%,
1000,2000,4000 times of dilutions are without deactivation.
Table 2 is that various concentration " lactic acid thimerosal (silkworm use) " acts on 30 minutes inactivation ratios to microsporidian
Table 2
| Extension rate | Action time (min) | Disease incidence (%) | Inactivation ratio (%) |
| 100 | 30 | 0 | 100 |
| 200 | 30 | 0 | 100 |
| 500 | 30 | 96 | 4 |
| 1000 | 30 | 100 | 0 |
| 2000 | 30 | 100 | 0 |
| 4000 | 30 | 100 | 0 |
| Negative control group | / | 100 | 0 |
| Blank control group | / | 0 | / |
3,300 and 400 times " lactic acid thimerosal (silkworm use) " the effect 30 minutes inactivation ratios to microsporidian
From the test result of front it is found that when diluting 1000 times or more, either act on 30 minutes or 60 minutes,
To nosema bombycis all without deactivation, and when diluting 500 times, 30 minutes inactivating efficacies are very low, it is necessary to act on 60
Minute can be only achieved 100% inactivation ratio.Therefore, in order to understand the inactivation when extension rate is lower than 500 times to microsporidian
Effect acts on 30 minutes inactivation tests carried out to microsporidian with 300,400 times of dilutions.As a result such as table 3,300,400
Times dilution has 100% inactivation ratio when acting on 30 minutes to microsporidian.
The inactivation ratio that table 3 is 300 and 400 times " lactic acid thimerosal (silkworm use) " effects 30 minutes to microsporidian
Table 3
| Extension rate | Action time (min) | Disease incidence (%) | Inactivation ratio (%) |
| 300 | 30 | 0 | 100 |
| 400 | 30 | 0 | 100 |
| Negative control group | / | 100 | 0 |
| Blank control group | / | 0 | / |
4, various concentration " lactic acid thimerosal (silkworm use) " acts on the deactivation to nosema bombycis in 15,5 minutes
By the test result of the above various concentration it is found that when 100,200,300,400 times of dilutions act on 30 minutes pair
Nosema bombycis has 100% inactivation ratio, in order to fully understand the above concentration when acting on the short period to micro- spore
The killing effect of sub- worm has carried out effect 15 minutes, the test of 5 minutes killing effects respectively.As a result such as table 4, table 5, either
Effect 15 minutes or effect 5 minutes, 100,200,300,400 times of " lactic acid thimerosal (silkworm use) " dilutions were to Nosema bombycis
Worm has 100% inactivation ratio.
4 various concentration of table " lactic acid thimerosal (silkworm use) " acts on 15 minutes inactivation ratios to microsporidian
Table 4
| Extension rate | Action time (min) | Disease incidence (%) | Inactivation ratio (%) |
| 100 | 15 | 0 | 100 |
| 200 | 15 | 0 | 100 |
| 300 | 15 | 0 | 100 |
| 400 | 15 | 0 | 100 |
| Negative control group | / | 100 | 0 |
| Blank control group | / | 0 | / |
5 various concentration of table " lactic acid thimerosal (silkworm use) " acts on 15 minutes inactivation ratio tables 5 to microsporidian
| Extension rate | Action time (min) | Disease incidence (%) | Inactivation ratio (%) |
| 100 | 5 | 0 | 100 |
| 200 | 5 | 0 | 100 |
| 300 | 5 | 0 | 100 |
| 400 | 5 | 0 | 100 |
| Negative control group | / | 100 | 0 |
| Blank control group | / | 0 | / |
(6) test evaluation
This test quantifies killing method using suspension, different to " lactic acid thimerosal (silkworm use) " different extension rates, effect
Time has carried out the test of system to the killing effect of nosema bombycis.Test result shows, 500 times and following dilution times
When number, " lactic acid thimerosal (silkworm use) " has good killing effect to nosema bombycis, and 500 times of dilutions need to act on
It can be only achieved within 60 minutes the complete killing effect to microsporidian, almost without effect at 30 minutes；400 times and following dilution times
There is 100% inactivation ratio in the action time of all test settings when number.
" lactic acid thimerosal (silkworm use) " is comprehensively considered to the killing effect of nosema bombycis and its characteristic etc., is recommended in reality
Border is bred silkworms, and production is upper to be carried out spraying or impregnates disinfection in use, when silkworm room rearing instrument and environment disinfected with 400 times of dilutions, guarantor
Wet 30 minutes or more；When mulberry leaf blade face sterilizes, (mulberry tree) or dipping (mulberry leaf) disinfection are sprayed with 400 times of dilution, is protected
Wet 10 minutes or more.
Claims (5)
1. a kind of lactic acid thimerosal, which is characterized in that the thimerosal by weight, including following component part:
Lactic acid content: 8.1wt%-12.3wt%；；
Neopelex 70.1wt%-90.1wt%.
2. a kind of lactic acid thimerosal as described in claim 1, which is characterized in that the lactic acid includes L lactic acid or DL lactic acid.
3. a kind of purposes of lactic acid thimerosal as described in claims 1 or 2 any one claim, which is characterized in that institute
Stating purposes is the deactivation to nosema bombycis.
4. a kind of purposes of lactic acid thimerosal as claimed in claim 4, which is characterized in that the deactivation is, by institute
It states the dilution of lactic acid thimerosal and is no more than 500 times, and carry out disinfection treatment not less than 60 minutes.
5. a kind of purposes of lactic acid thimerosal as claimed in claim 4, which is characterized in that the deactivation is, by institute
It states the dilution of lactic acid thimerosal and is no more than 400 times, and carry out disinfection treatment not less than 5 minutes.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023219867A1 (en) * | 2022-05-11 | 2023-11-16 | Arxada Ag | Single dose disinfectant |
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2019
- 2019-02-22 CN CN201910133692.XA patent/CN109874791A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023219867A1 (en) * | 2022-05-11 | 2023-11-16 | Arxada Ag | Single dose disinfectant |
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Application publication date: 20190614 |