CN109673646B

CN109673646B - Antiviral composition containing amino oligosaccharin and application thereof

Info

Publication number: CN109673646B
Application number: CN201910112038.0A
Authority: CN
Inventors: 安娟; 叶宇平
Original assignee: Foshan Hailiying Biotechnology Co ltd
Current assignee: Foshan Hailiying Biotechnology Co ltd
Priority date: 2019-02-13
Filing date: 2019-02-13
Publication date: 2023-07-28
Anticipated expiration: 2039-02-13
Also published as: CN109673646A

Abstract

The invention relates to an antiviral composition for resisting viruses and application thereof, in particular to an antiviral composition containing amino-oligosaccharin and ningnanmycin, wherein the weight ratio of the amino-oligosaccharin to the ningnanmycin is 1:2-1:8, and especially when the ratio of the amino-oligosaccharin to the ningnanmycin is 1:3, the effect of preventing and treating viral diseases is optimal, and the content of the amino-oligosaccharin and the ningnanmycin accounts for 0.1-15 percent, preferably 1-10 percent of the weight of the composition; the active ingredients of the antiviral composition have synergistic effect in a certain proportion, and the duration of the antiviral composition is longer than that of a single agent.

Description

Antiviral composition containing amino oligosaccharin and application thereof

Technical Field

The invention belongs to the field of biological pesticides, and in particular relates to an antiviral composition containing amino-oligosaccharin and ningnanmycin and application thereof.

Background

Tomato virus diseases are one of important diseases in tomato production and occur in various places in China, at present, along with the development of facility pesticides, the virus diseases are more and more serious on economic crops, the number of plant viruses is large, the hazard area is wide, the economic loss is large and the prevention and the control are difficult, at present, the prevention and the control of the virus diseases are mainly realized by chemical pesticides, a new environment-friendly medicament amino oligosaccharin for preventing and controlling the virus diseases is prepared from chitin in marine organism shells through deacetylation and enzymatic degradation, the novel oligosaccharide biological pesticide can prevent and control various plant fungal diseases and virus diseases, and the amino oligosaccharin has various mechanisms in the aspects of disease prevention and disease resistance as biological pesticide, can be used as a plant growth agent besides being used as an active signal molecule for inducing plant bodies to generate resistance, promotes the activation of plant cells and stimulates the growth of plants, and has the double effects of a pesticide fertilizer. Although the amino-oligosaccharins have certain application in agriculture, such as plant pathogen growth inhibitors, disease resistance inducers of crops, seed treatment agents and the like, the amino-oligosaccharins can delay or relieve symptoms, cannot effectively eradicate viruses, and have obvious use effects in short term because of unique action mechanisms and are not expressed by chemical preparations or chemical pesticides, so the amino-oligosaccharins are not promoted by farmers and agricultural material distributors all the time, thereby limiting popularization and application of the amino-oligosaccharins. Reasonable compound composition and timely medication are important factors for fully playing the role of prevention and treatment. Therefore, how to utilize the amino oligosaccharin to thoroughly eradicate plant virus diseases, ensure the healthy growth of plants and improve the yield is an urgent problem to be solved.

Ningnanmycin, the name Ningnanmycin, is an innovative biological pesticide developed by the institute of Chengdu biological research of the national academy of sciences, is a novel cytosine nucleoside peptide antibiotic, and is mainly used for preventing and treating crop virus diseases such as tomatoes, peppers, tobacco and the like at present by prolonging the incubation period of bacteria, destroying the virion structure, reducing the virion concentration and regulating the metabolism of organisms so as to enhance the disease resistance and immunity of plants.

In the prior art, a pesticide composition prepared by compounding ningnanmycin and amino-oligosaccharin is disclosed in patent CN107549170A, a ternary compound bactericide containing the amino-oligosaccharin, the ningnanmycin and the Xinjunan acetate is disclosed, a synergistic effect is achieved after ternary compound, a control medicament for describing the compounding of the amino-oligosaccharin and the ningnanmycin is disclosed in specification table 2, however, the synergistic effect is achieved after the amino-oligosaccharin and the ningnanmycin are not disclosed.

Through a large number of experiments, the inventor of the application finds that the amino-oligosaccharin and Ningnanmycin have a synergistic effect on preventing and treating viral diseases after being compounded in certain specific ranges, and the invention is completed through further researches.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide a synergistic pesticide composition for resisting plant viruses, and simultaneously, the pesticide composition containing amino-oligosaccharin and ningnanmycin can promote plant growth, and is safe and environment-friendly.

Another object of the present invention is to provide an application of the pesticide composition, which is widely applied to prevention and treatment of various viral diseases such as tomato virus disease, potato virus disease, watermelon virus disease, pepper virus disease, tobacco mosaic virus, etc.

In order to achieve the above purpose, the invention adopts the following technical scheme:

an antiviral composition is characterized in that the active ingredients comprise amino-oligosaccharin and ningnanmycin, wherein the weight ratio of the amino-oligosaccharin to the ningnanmycin is 1:2-1:8, and especially when the ratio of the amino-oligosaccharin to the ningnanmycin is 1:3, the effect of preventing and treating viral diseases is optimal.

Further, the content of the amino-oligosaccharin and the ningnanmycin accounts for 0.1-15% of the weight of the composition, and is preferably 1-10%.

Furthermore, the antiviral composition of the invention can be prepared into any formulation suitable for agriculture, including but not limited to water aqua, soluble agent, wettable powder, water dispersible granule, microemulsion and the like.

Further, the antiviral composition of the present invention is usually applied by spraying, and other application techniques suitable for agricultural use, such as treating seeds with the pesticidal composition of the present invention, including but not limited to dressing, dipping, immersing seeds, etc., may be applied as needed.

Further, the antiviral composition of the present invention may be added with other agricultural active ingredients such as bactericides, pesticides, plant growth regulators, fertilizers and the like.

Compared with the prior art, the antiviral composition has the beneficial effects that:

1. a large number of experiments show that the amino-oligosaccharin has a synergistic effect on the prevention and treatment of viral diseases under a specific proportion, so that the use amount of active ingredients can be reduced, and the prevention and treatment effect is improved;

2. the antiviral composition has good control effect, low pesticide residue, stable pesticide effect and difficult resistance generation;

3. the antiviral composition can delay the generation of drug resistance of viral diseases to medicaments, and can prolong the service life of pesticide active ingredients.

Detailed Description

The invention is further illustrated with reference to the following examples, in which the percentages are by weight, but the invention is by no means limited to these examples. The following examples are given for the purpose of illustrating the invention only and are not to be construed as limiting the scope of the invention. It should be noted that any modification, equivalent replacement or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention, and thus, the protection scope of the present invention patent should be subject to the appended claims.

1. Formulation examples

1. Example 1:8% amino oligosaccharin Ningnan mycin aqua (1:3)

The preparation method of the water aqua comprises the following steps: the active ingredients, various auxiliary agents, solvents and the like are uniformly mixed in a mixing kettle according to the corresponding proportion according to the formula, and the aqueous agent of the invention can be prepared.

2. EXAMPLE 2 10% amino oligosaccharin Ningnanmycin aqua (1:4)

3. EXAMPLE 3 4% amino oligosaccharin Ningnanmycin aqua (1:1)

4. EXAMPLE 4 10% amino oligosaccharin Ningnanmycin aqua (1:9)

5. Example 5:9% amino oligosaccharin Ningnan mycin aqua (2:1)

6. Example 6:8% amino oligosaccharin Ningnanmycin solution (1:3)

The preparation method of the soluble agent comprises the following steps: the active ingredients, various auxiliary agents, solvents and the like are stirred uniformly in a mixing kettle according to the proportion according to the formula, and the soluble agent of the invention can be prepared.

2. Bioassays

1. Control object: tomato virus disease;

2. test agent: (1) treatment group: the antiviral composition formulations of examples 1-6; (2) Control group 1:2% amino oligosaccharin aqua (Hebei Guanlong Agrochemical Co., ltd.); (3) control group 2:8% Ningnanmycin aqua (Deqiang Biotechnology Co., ltd.).

3. Test design and application method: the plant is experimentally arranged in a green house of Fan Wangcun vegetables, 200 m long and 8 m wide, cultivated with new substrate, 50cm long and 30 cm wide, one plant is carried on each end of each pot, the pots are arranged in rows, the row length is 20m, and the distance between the pots is not left 40cm, and the space between the pots is not left. Pouring the treatment group medicament and the control group medicament into a measuring cup according to the required amount of test design, pouring the mixture into a sprayer with a certain amount of water after stirring uniformly, pouring the mixture into the sprayer after washing the measuring cup cleanly with water, and finally adding water to the required amount of test (namely, 2 times of dilution method), wherein each treatment is carried out by adding water into a kavas electrostatic sprayer for spraying, and the medicament does not leave residual liquid.

The test method comprises the following steps: area of each cell 40m ² The disease index was investigated and the control effect was calculated at 3, 7 and 14 days after the administration, by taking points on the diagonal line of each cell, extracting 10 plants of investigation from each point, and classifying the disease as follows:

level 0: no pathology;

stage 1: mingmai and Calomelas;

3 stages: heart leaf and middle leaf flower leaf;

5 stages: heart leaves and middle leaf flowers and leaves, few leaves are deformed, shrunken or plants are slightly dwarfed;

7 stages: heavy flower leaves, most of the leaves are deformed, shrunken or plant dwarf;

stage 9: heavy flower leaves, obviously deformed leaves, linear leaves, severely dwarfed plants and even death.

The test results are shown in table 1:

TABLE 1 prevention and treatment effects of different agents on tomato virus diseases

From the test results in table 1, the antiviral compositions of the amino-oligosaccharins and the ningnanmycin in examples 1 and 2 are obviously better than other test groups, which shows that when the compounding ratio of the amino-oligosaccharins and the ningnanmycin is 1:3 and 1:4, the control effect of the antiviral composition shows a synergistic effect compared with that of a single agent. Especially, the antiviral composition of example 1 has longer lasting period, and the control effect on tomato virus diseases is still more than 90% after 14 days of administration. In addition, in the application range, the antiviral composition disclosed by the invention is not found to be harmful to tomato crops, so that the antiviral composition disclosed by the invention is good in safety, and in conclusion, the antiviral composition disclosed by the invention has the characteristics of high safety and good prevention effect, and is worthy of popularization and application in production.

Claims

1. The application of the aqueous solution containing the amino-oligosaccharins and the ningnanmycin for preventing and treating tomato virus diseases is characterized in that the aqueous solution containing the amino-oligosaccharins and the ningnanmycin comprises the following components:

amino oligosaccharin 2%

Ningnanmycin 6%

Sodium dodecyl benzene sulfonate 10%

Dispersant NNO 5%

And the balance of deionized water.