CN103404519B - A kind of microbicide compositions and application thereof - Google Patents
A kind of microbicide compositions and application thereof Download PDFInfo
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- CN103404519B CN103404519B CN201310315189.9A CN201310315189A CN103404519B CN 103404519 B CN103404519 B CN 103404519B CN 201310315189 A CN201310315189 A CN 201310315189A CN 103404519 B CN103404519 B CN 103404519B
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- fluoxastrobin
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Abstract
The invention discloses a kind of microbicide compositions and application thereof, belong to field of vegetable disease control.Said composition comprises Fluoxastrobin, chitosan, the weight ratio of Fluoxastrobin and chitosan is 1 ~ 10: 2 ~ 30, can be used for preventing and treating the melon and fruit that caused by Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti and vegetable disease, have bactericidal effect good, crop resistance, the usage amount reducing chemical pesticide and access times can be improved, not easily the feature such as to develop immunity to drugs.
Description
Technical field
The present invention relates to field of vegetable disease control, specifically a kind of microbicide compositions and application thereof.
Background technology
Fluoxastrobin, English name: Azoxystrobin.The former medicine of Fluoxastrobin is brown solid, fusing point 114-116 DEG C, and sterling is white crystal, fusing point 116 DEG C, relative density 1.34, vapour pressure 1.1x10
-7mPa (20 DEG C).In water, solvability 6mg/L (20 DEG C), is dissolved in methyl alcohol, toluene, acetone, is slightly soluble in n-octyl alcohol, hexane, be soluble in ethyl acetate, acetonitrile, carrene.In the aqueous solution, Photolysis Half is 2 weeks, to hydrolysis-stable.Rat, chmice acute LD50 of passing through mouth (male, female) > 5000mg/kg, rat acute sucks LC50 (mg/L): male 0.96, female 0.69, ichthyotoxin LC50 (96h, mg/L) trout 0.47, carp 1.6, reaches sunfish 1.1, earthworm LC50 (14d) 283mg/kg; Minimal irritation effect is had to rabbit skin and eyes; Without teratogenesis, carcinogenic mutagenesis; Belong to methoxy acrylic bactericide, its mechanism of action suppresses mitochondrial breathing by electro transfer between thin portion pigment b and c, extranuclear mitochondria is mainly through breathing as cell provides energy (ATP), if mitochondrial respiratory is obstructed, can not produce ATP, cell will be dead; There is the bactericidal activity of wide spectrum, all have good activity to nearly all fungi (Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti) disease such as powdery mildew, rust, glume blight, net blotch, scab, downy mildew, rice blast etc.Although Fluoxastrobin have fungicidal spectrum wide, increase disease resistance, improve resistance against disadvantage, delay senility, the feature such as the lasting period is long, drug cost is high, should not with other chemical pesticide mixtures.
Chitosan, also claims chitin, chitosan, and the polysaccharide material be made up of aminoglucose and n acetylglucosamine n polymer, is present in the epidermis of shellfish in the Nature, the pin shell of marine organisms shrimp crab, and in the cell wall of the mushroom material such as auricularia auriculajudae mushroom.Chitosan forms diaphragm at plant surface, prevents germ invasion; Seedling healthy growth can be protected, promote the flower bud differentiation in root system development and early stage, cultivate and improve crop resistance strong sprout; Floral organ is grown normal, reduce deformed fruit.Effectively prevent the generation of crop pest, reduce dosage and times for spraying, degrading pesticide residues.The killing action certain to the tool such as blight, bacterial wilt, virus disease, spot blight, shot hole, epidemic disease mould class fungal disease of cotton, but singlely there is sterilization lasting period short deficiency when using.
Summary of the invention
Technical assignment of the present invention is for above-mentioned the deficiencies in the prior art, provides a kind of microbicide compositions.Said composition have bactericidal effect good, crop resistance can be improved, reduce the usage amount of chemical pesticide and access times, the feature such as not easily develop immunity to drugs.
Another technical assignment of the present invention is to provide the application of above-mentioned microbicide compositions.
Technical assignment of the present invention realizes in the following manner: a kind of microbicide compositions, is characterized in, comprises Fluoxastrobin, chitosan, and the weight ratio of Fluoxastrobin and chitosan is 1 ~ 10: 2 ~ 30.
The preferred weight ratio of Fluoxastrobin and chitosan is 2.7 ~ 3.6: 10 ~ 12.
The optimum weight ratio of Fluoxastrobin and chitosan is 3.6: 12.
Combinations thereof medicine can be mixed with suspending agent, wetting powder, aqua etc. with various auxiliary combination of the prior art, preferred following wetting powder: the composition including the claim 1 of effective amount, and one or more and Fluoxastrobin, chitosan are made into auxiliary agent, the carrier of wetting powder.
Described auxiliary agent comprises dispersant, wetting agent etc., all can select usual auxiliaries of the prior art.As dispersants such as NNO, sodium lignin sulfonate, sodium phosphate trimers; The wetting agents such as the withered powder of benzene, spaonin powder, dodecyl sodium sulfate.
Described carrier can select the various common carrier of prior art, as potter's clay, kaolin, precipitated calcium carbonate, white carbon etc.
Confirm through applicant's great many of experiments, Fluoxastrobin and chitosan microbicide compositions have good bactericidal effect, can be applicable to control melon and fruit and vegetable disease, described disease is mainly by the fungus-caused cucumber downy mildew of Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti, tomato late blight, potato late blight, powdery mildew of cucumber, capsicum epidemic disease, pepper powdery mildew or peronophythora litchi.
When using Fluoxastrobin and chitosan microbicide compositions to prevent and treat melon and fruit and vegetable disease, described microbicide compositions is with after the water-soluble solution of 600-800 times of weight, and even spraying is in blade.
Microbicide compositions of the present invention compared with prior art has following outstanding beneficial effect:
(1) Fluoxastrobin and the mutual mixture of chitosan can not produce conflict, and synergistic effect is obvious, can significantly reduce Pesticide use amount, highly effective and safe, reduce costs;
(2) unharmful substance remains, and also can improve crop self resistance against diseases, meet the standard of pollution-free food, organic food.
Embodiment
With specific embodiment, microbicide compositions of the present invention and application thereof are described in detail below.
Preparation embodiment one:
By weight ratio be 3.6: 12 Fluoxastrobin mix with chitosan, obtain microbicide compositions finished product.
Preparation embodiment two:
By weight ratio be 4: 13 Fluoxastrobin mix with chitosan, obtain microbicide compositions finished product.
Preparation embodiment three:
By weight ratio be 6: 24 Fluoxastrobin mix with chitosan, obtain microbicide compositions finished product.
Preparation embodiment four:
Be that the Fluoxastrobin of 3.6: 12: 1: 1.5: 81.9, chitosan, NNO, dodecyl sodium sulfate and precipitated calcium carbonate mix by weight ratio, be crushed to 300 orders, obtain wetting powder finished product.
Preparation embodiment five:
Be that the Fluoxastrobin of 3.6: 12: 2.5: 1.5: 80.4, chitosan, sodium phosphate trimer, the withered powder of benzene and kaolin mix by weight ratio, be crushed to 300 orders, obtain wetting powder finished product.
Test example one: preparation embodiment one, two and three gained microbicide compositions is to bacterium of downy mildew of cucumber indoor pharmacodynamic test
Fluoxastrobin and chitosan are mixed to get microbicide compositions according to the weight ratio of 3.6: 12,4: 13,6: 24 respectively.
Utilize leaf dish method to carry out Indoor antibacterial mensuration, respectively composition, 250g/L Fluoxastrobin and 60g/L chitosan are mixed with the liquid that concentration is 100ppm, 50ppm, 10ppm, 1ppm, 0.1ppm respectively; Choose the fresh blade of cucumber and break into the leaf dish that diameter is 1.5cm, directly immersing in variable concentrations liquid, each concentration 10 leaf dish; After drying, each leaf dish drips 60 μ l sporangia suspensions.Putting into 21 DEG C of constant incubator moisturizings to cultivate, observe after 5d, there is the leaf dish number of scab in record, calculates inhibiting rate, analyzes EC
50and confidence interval.
Inhibiting rate (%)=(contrast scab number-process scab number)/contrast scab number × 100
Table 1 microbicide compositions is to the suppression situation of downy mildew
As can be seen from table, weight ratio be 3.6: 12,4: 13,6: 24 Fluoxastrobin and chitosan composition in bacterium of downy mildew of cucumber indoor pharmacodynamic test, EC
50value is respectively 2.7086mg/L, 1.3924mg/L and 1.2456mg/L, the EC of 250g/L Fluoxastrobin suspending agent and 60g/L chitosan aqua
50value is respectively 7.3951mg/L and 23.8323mg/L, illustrates that Fluoxastrobin and chitosan composition have significant inhibitory action to Pseudoperonospora cubensis, and has synergistic effect.
Test example two: preparation embodiment four gained wetting powder is to cucumber downy mildew field control effectiveness test
Be that the Fluoxastrobin of 3.6: 12: 1: 1.5: 81.9, chitosan, NNO, dodecyl sodium sulfate and precipitated calcium carbonate mix by weight ratio, be crushed to 300 orders, obtain wetting powder finished product.
In November ,-2012 in March, 2012 carries out cucumber downy mildew field control effectiveness test in Dezhou, Shandong academy of agricultural sciences experimental field winter warm type big shack, this wetting powder 600 times of liquid, 800 times of liquid, 1000 times of liquid are set, medicament contrast is 250g/L Fluoxastrobin suspending agent 1500 times of liquid and 60g/L chitosan aqua 800 times of liquid, blank is clear water spraying, respectively on spring stubble and autumn stubble two season cucumber, test adopts the group arrangement of random district, and plot area is about 13.2m
2, each process repetition 3 times, respectively before dispenser, after dispenser, 7d, 14d investigate disease index and control efficiency.
Method with reference to Ministry of Agriculture's agricultural chemicals field trial criterion bactericidal agent for preventing and treating cucumber downy mildew bactericide carries out investigation statistics analysis.
Table 2 microbicide compositions control cucumber downy mildew field trial disease index
1. respectively process disease index and be 3 repetition mean values; 2. Notable difference test adopts " DMRT " method to carry out; 3. the significance of difference compares employing letter representation method, and all same letter persons represent that difference is not remarkable in same level.
Table 3 microbicide compositions is to cucumber downy mildew field trial control efficiency
1. respectively process control efficiency and be 3 repetition mean values; 2. Notable difference test adopts " DMRT " method to carry out; 3. the significance of difference compares employing letter representation method, and all same letter persons represent that difference is not remarkable in same level.
As can be seen from table, Fluoxastrobin and chitosan composition are in spring stubble and autumn stubble cucumber downy mildew control, concentration be 600 times of liquid and 800 times of liquid after administration the disease index of 14 days be respectively 13.37 (spring stubble cucumber) and 19.59 (autumn stubble cucumber), control efficiency is respectively 81.79% (spring stubble cucumber) and 75.49% (autumn stubble cucumber), concentration be 800 times of liquid after administration the disease index of 14 days be respectively 14.79 (spring stubble cucumber) and 23.63 (autumn stubble cucumber), control efficiency is respectively 79.86% (spring stubble cucumber) and 70.44% (autumn stubble cucumber), compared with the contrast medicament of peer-level, significantly be better than contrast medicament 250g/L Fluoxastrobin and 60g/L chitosan, preventive effect significantly improves.
Example three prepares embodiment five gained wetting powder to tomato late blight field control effectiveness test
Be that the Fluoxastrobin of 3.6: 12: 2.5: 1.5: 80.4, chitosan, sodium phosphate trimer, the withered powder of benzene and kaolin mix by weight ratio, be crushed to 300 orders, obtain wetting powder finished product.
In December ,-2012 in March, 2012 carries out tomato late blight field control effectiveness test in Front St village, Cui Zhai town, Jiyang County, Jinan City, Shandong Province booth, this wetting powder 600 times of liquid, 800 times of liquid, 1000 times of liquid are set, medicament contrast is 250g/L Fluoxastrobin suspending agent 1500 times of liquid and 60g/L chitosan aqua 800 times of liquid, blank is clear water spraying, respectively on spring stubble and autumn stubble two season tomato, test adopts the group arrangement of random district, and plot area is about 13.2m
2, each process repetition 3 times, respectively before dispenser, after dispenser, 7d, 14d investigate disease index and control efficiency.
With reference to Ministry of Agriculture agricultural chemicals field trial criterion bactericidal agent for preventing and treating tomato late blight bactericide method carry out investigation statistics analysis.
Table 4 microbicide compositions control tomato late blight field trial disease index
1. respectively process disease index and be 3 repetition mean values; 2. Notable difference test adopts " DMRT " method to carry out; 3. the significance of difference compares employing letter representation method, and all same letter persons represent that difference is not remarkable in same level.
Table 5 microbicide compositions is to tomato late blight field trial control efficiency
1. respectively process control efficiency and be 3 repetition mean values; 2. Notable difference test adopts " DMRT " method to carry out; 3. the significance of difference compares employing letter representation method, and all same letter persons represent that difference is not remarkable in same level.
As can be seen from table, Fluoxastrobin and chitosan composition are in spring stubble and autumn stubble tomato late blight control, concentration be 600 times of liquid and 800 times of liquid after administration the disease index of 14 days be respectively 17.23 (spring stubble tomatoes) and 15.53 (autumn stubble tomatoes), control efficiency is respectively 80.43% (spring stubble tomato) and 81.93% (autumn stubble tomato), concentration be 800 times of liquid after administration the disease index of 14 days be respectively 19.71 (spring stubble tomatoes) and 21.36 (autumn stubble tomatoes), control efficiency is respectively 77.62% (spring stubble tomato) and 75.14% (autumn stubble tomato), compared with the contrast medicament of peer-level, significantly be better than contrast medicament 250g/L Fluoxastrobin and 60g/L chitosan, preventive effect significantly improves.
Microbicide compositions of the present invention has obvious synergistic effect, is advisable in use with 600-800 times of liquid concentration, and control period distances does not exceed 21 days.
Claims (5)
1. a microbicide compositions, is characterized in that, the active ingredient of this microbicide compositions is made up of Fluoxastrobin and chitosan, and the weight ratio of Fluoxastrobin and chitosan is 2.7 ~ 3.6: 10 ~ 12.
2. microbicide compositions according to claim 1, is characterized in that, the weight ratio of Fluoxastrobin and chitosan is 3.6: 12.
3. microbicide compositions wetting powder, is characterized in that, includes the composition of the claim 1 of effective amount, and one or more and Fluoxastrobin, chitosan are made into the auxiliary agent of wetting powder.
4. the application of the microbicide compositions described in claim 1 or 2 in control melon and fruit and vegetable disease, described disease is by the fungus-caused cucumber downy mildew of Ascomycetes, Basidiomycetes, Oomycete and Fungi Imperfecti, tomato late blight, potato late blight, powdery mildew of cucumber, capsicum epidemic disease, pepper powdery mildew or peronophythora litchi.
5. apply according to claim 4, it is characterized in that: described microbicide compositions is with after the water-soluble solution of 600-800 times of weight, and even spraying is in blade.
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| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CN101836633A (en) * | 2010-05-14 | 2010-09-22 | 深圳市朗钛生物科技有限公司 | Pesticide composition containing amino-oligosaccharide and kresoxim-methyl and use method and application thereof |
| WO2012076563A1 (en) * | 2010-12-08 | 2012-06-14 | Basf Se | Fungicidal mixtures |
| CN102617224A (en) * | 2012-03-07 | 2012-08-01 | 海南正业中农高科股份有限公司 | Plant wound protective agent containing chitosan oligosaccharide |
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| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CN101836633A (en) * | 2010-05-14 | 2010-09-22 | 深圳市朗钛生物科技有限公司 | Pesticide composition containing amino-oligosaccharide and kresoxim-methyl and use method and application thereof |
| WO2012076563A1 (en) * | 2010-12-08 | 2012-06-14 | Basf Se | Fungicidal mixtures |
| CN102617224A (en) * | 2012-03-07 | 2012-08-01 | 海南正业中农高科股份有限公司 | Plant wound protective agent containing chitosan oligosaccharide |
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Effective date of registration: 20230801 Address after: 844200 Cultural Square, Tashiqi Airike Village (11 Village), Tazihong Township, Shule County, Kashgar Prefecture, Xinjiang Uygur Autonomous Region Shandong Aid Xinjiang Science and Technology Hut Patentee after: Xinjiang Jinwo Agricultural Technology Co.,Ltd. Address before: Room 614, Plant Protection Research Institute, Shandong Academy of Agricultural Sciences, Building 1, No. 202 Gongye North Road, Licheng District, Jinan City, Shandong Province, 250100 Patentee before: INSTITUTE OF PLANT PROTECTION, SHANDONG ACADEMY OF AGRICULTURAL SCIENCES |