CN107318844B - Anti-phytoplasma composition containing lentinan - Google Patents
Anti-phytoplasma composition containing lentinan Download PDFInfo
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- CN107318844B CN107318844B CN201710644318.7A CN201710644318A CN107318844B CN 107318844 B CN107318844 B CN 107318844B CN 201710644318 A CN201710644318 A CN 201710644318A CN 107318844 B CN107318844 B CN 107318844B
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- lentinan
- phytoplasma
- oligosaccharin
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- 229920001491 Lentinan Polymers 0.000 title claims abstract description 47
- 229940115286 lentinan Drugs 0.000 title claims abstract description 47
- 239000000203 mixture Substances 0.000 title claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000004480 active ingredient Substances 0.000 claims abstract description 8
- 238000005507 spraying Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 3
- 239000007864 aqueous solution Substances 0.000 claims description 23
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 239000002552 dosage form Substances 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 201000010099 disease Diseases 0.000 abstract description 28
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 28
- 241001468265 Candidatus Phytoplasma Species 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 13
- 244000144972 livestock Species 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 17
- 238000012360 testing method Methods 0.000 description 14
- 239000003814 drug Substances 0.000 description 11
- 241001247821 Ziziphus Species 0.000 description 10
- 244000126002 Ziziphus vulgaris Species 0.000 description 10
- 235000008529 Ziziphus vulgaris Nutrition 0.000 description 10
- 241001481828 Glyptocephalus cynoglossus Species 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 244000007853 Sarothamnus scoparius Species 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 6
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 6
- 229920001285 xanthan gum Polymers 0.000 description 6
- 239000000230 xanthan gum Substances 0.000 description 6
- 229940082509 xanthan gum Drugs 0.000 description 6
- 235000010493 xanthan gum Nutrition 0.000 description 6
- 238000001802 infusion Methods 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 230000001502 supplementing effect Effects 0.000 description 5
- 229920000056 polyoxyethylene ether Polymers 0.000 description 4
- 229940051841 polyoxyethylene ether Drugs 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- ULUAUXLGCMPNKK-UHFFFAOYSA-N Sulfobutanedioic acid Chemical compound OC(=O)CC(C(O)=O)S(O)(=O)=O ULUAUXLGCMPNKK-UHFFFAOYSA-N 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 239000004563 wettable powder Substances 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 239000005917 Methoxyfenozide Substances 0.000 description 2
- 241000203073 Phytoplasma sp. Species 0.000 description 2
- -1 Polyoxyethylene monostearate Polymers 0.000 description 2
- 239000004721 Polyphenylene oxide Substances 0.000 description 2
- 239000005929 Spinetoram Substances 0.000 description 2
- GOENIMGKWNZVDA-OAMCMWGQSA-N Spinetoram Chemical compound CO[C@@H]1[C@H](OCC)[C@@H](OC)[C@H](C)O[C@H]1OC1C[C@H]2[C@@H]3C=C4C(=O)[C@H](C)[C@@H](O[C@@H]5O[C@H](C)[C@H](CC5)N(C)C)CCC[C@H](CC)OC(=O)CC4[C@@H]3CC[C@@H]2C1 GOENIMGKWNZVDA-OAMCMWGQSA-N 0.000 description 2
- 241001414989 Thysanoptera Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 235000006545 Ziziphus mauritiana Nutrition 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- XMEVHPAGJVLHIG-FMZCEJRJSA-N chembl454950 Chemical compound [Cl-].C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H]([NH+](C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O XMEVHPAGJVLHIG-FMZCEJRJSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- QCAWEPFNJXQPAN-UHFFFAOYSA-N methoxyfenozide Chemical compound COC1=CC=CC(C(=O)NN(C(=O)C=2C=C(C)C=C(C)C=2)C(C)(C)C)=C1C QCAWEPFNJXQPAN-UHFFFAOYSA-N 0.000 description 2
- 229920000570 polyether Polymers 0.000 description 2
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 229960004989 tetracycline hydrochloride Drugs 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 238000004383 yellowing Methods 0.000 description 2
- BITDLWAQPKSXTF-UHFFFAOYSA-M 1-[(3-nitrophenyl)methoxymethyl]pyridin-1-ium;chloride Chemical compound [Cl-].[O-][N+](=O)C1=CC=CC(COC[N+]=2C=CC=CC=2)=C1 BITDLWAQPKSXTF-UHFFFAOYSA-M 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 241001414720 Cicadellidae Species 0.000 description 1
- 241001466042 Fulgoromorpha Species 0.000 description 1
- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 244000055346 Paulownia Species 0.000 description 1
- 235000003417 Plumeria rubra f acutifolia Nutrition 0.000 description 1
- 244000040691 Plumeria rubra f. acutifolia Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- KIPLYOUQVMMOHB-MXWBXKMOSA-L [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O Chemical compound [Ca++].CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O.CN(C)[C@H]1[C@@H]2[C@@H](O)[C@H]3C(=C([O-])[C@]2(O)C(=O)C(C(N)=O)=C1O)C(=O)c1c(O)cccc1[C@@]3(C)O KIPLYOUQVMMOHB-MXWBXKMOSA-L 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000019693 cherries Nutrition 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- JMGZBMRVDHKMKB-UHFFFAOYSA-L disodium;2-sulfobutanedioate Chemical compound [Na+].[Na+].OS(=O)(=O)C(C([O-])=O)CC([O-])=O JMGZBMRVDHKMKB-UHFFFAOYSA-L 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- LRMHFDNWKCSEQU-UHFFFAOYSA-N ethoxyethane;phenol Chemical compound CCOCC.OC1=CC=CC=C1 LRMHFDNWKCSEQU-UHFFFAOYSA-N 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- IOVNUMWQKJVCHJ-UHFFFAOYSA-N guanidine morpholine hydrochloride Chemical compound NC(=N)N.N1CCOCC1.Cl IOVNUMWQKJVCHJ-UHFFFAOYSA-N 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 229960002581 moroxydine hydrochloride Drugs 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940063650 terramycin Drugs 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Abstract
The invention provides an anti-phytoplasma composition containing lentinan, which comprises active ingredients of lentinan and amino-oligosaccharin; the weight ratio of the lentinan to the amino-oligosaccharin is 1:20-20: 1; the total content of the two is 0.1-20 w.t%, and the balance is auxiliary materials. The application method of the anti-phytoplasma composition is spraying or injecting into a plant transportation system by adding water. The anti-phytoplasma composition containing the lentinan has the effects of preventing and treating phytoplasma diseases, has good effect and high field control effect, and the effective components are safe to plants, people and livestock.
Description
Technical Field
The invention relates to a composition for resisting phytoplasma, in particular to a composition consisting of lentinan and amino-oligosaccharin applied to phytoplasma plant diseases, belonging to the field of plant chemical protection.
Background
Phytoplasma (phytoplasma), originally called mycoplasma-like organism (MLO), is a unicellular prokaryotic organism without a cell wall, surrounded by a biofilm. The phytoplasma can not be cultured artificially and colonizes tissues such as plant sieve tube cells and intestinal tracts, lymph, salivary glands and the like of piercing-sucking medium insects. Many plant diseases that were once thought to be caused by viruses have been identified as being caused by phytoplasmas. Phytoplasma is mainly transmitted by insects which graft or eat phloem of plants, including leafhoppers, plant hoppers and aphids, and often causes yellowing, leaf change and arbuscular division of the plants, thereby causing huge loss to economic crops.
At present, the phytoplasma is found to be linked with more than 1000 diseases on plants to cause serious diseases of economic forest trees such as wheat dwarf, sweet potato arbuscular disease, rose mosaic disease, jujube witches broom disease, pagodatree arbuscular disease, cherry lethal yellowing disease, paulownia arbuscular disease and the like, such as a plurality of important grain crops, vegetables, ornamental plants, fruit trees and the like, and more than 100 kinds of related plant diseases have been reported in continents of China. The diseases are wide in distribution range, high in propagation speed and strong in pathogenicity, and once the diseases are infected, the diseases are difficult to cure by using the traditional disease control technology. At present, the prevention and treatment of phytoplasma mainly depend on tetracycline, terramycin and other antibiotic medicaments, at present, the field treatment of the diseases of the phytoplasma such as jujube witches broom and the like is mostly antibiotic medicament injection treatment, but the disappearance of symptoms of the diseases of the phytoplasma due to the application of antibiotics is mostly temporary and usually recurs after a period of time; moreover, multiple research results show that antibiotic medicaments have potential safety influence on plants and can cause the growth of the plants to be inhibited.
The amino-oligosaccharin is oligosaccharide with D-aminoglucose connected with β -1,4 glycosidic bond, and can inhibit the growth of some pathogenic bacteria, influence the germination of fungal spore, induce variation of hypha form and change of spore biochemical, excite the gene in plant body, produce chitinase, glucanase and disease course related protein, etc. and have cell activating effect, so that it is favorable to the recovery of damaged plant, promote root growth, strengthen crop resistance and promote plant growth.
Disclosure of Invention
The invention provides an anti-phytoplasma composition containing lentinan, aiming at the problems of less effective medicament and poor safety in preventing and treating phytoplasma diseases, and the composition has good biological activity and high safety to crops.
In order to achieve the purpose, the invention adopts the following technical scheme.
An anti-phytoplasma composition containing lentinan comprises lentinan and amino-oligosaccharin as active ingredients; the weight ratio of the lentinan to the amino-oligosaccharin is 1:20-20: 1.
The total content of active components of the anti-phytoplasma composition, namely lentinan and amino-oligosaccharin, is 0.1-20 w.t%, and the balance is auxiliary materials.
Preferably, the active ingredients lentinan: the weight ratio of the amino-oligosaccharin is 1:10-10:1, and more preferably 3:8-8: 3; the total content of the two is 0.5-5 w.t%.
The dosage form of the anti-phytoplasma composition is selected from one of Soluble Powder (SP), Soluble Liquid (SL) or Aqueous Solution (AS), and the most preferable is aqueous solution.
The application method of the anti-phytoplasma composition is spraying or injecting into a plant transportation system by adding water.
The invention has the following beneficial advantages: the anti-phytoplasma composition containing the lentinan has the effects of preventing and treating phytoplasma diseases, has good effect and high field control effect, and the effective components are safe to plants, people and livestock.
Detailed Description
The present invention will be further illustrated with reference to the following examples, but the present invention is not limited to the following examples.
EXAMPLE 1 preparation of a preparation of lentinan and amino-oligosaccharin
Aqueous solution of 1.15% lentinan and amino-oligosaccharin (3: 8)
The weight percentage composition is as follows:
lentinan 1.36%
Amino-oligosaccharin 3.64%
Sodium dodecyl benzene sulfonate 1%
Styryl phenyl polyoxyethylene ether 4%
6 percent of ammonium sulfate
Supplementing water to 100%
Preparation:
dissolving ammonium sulfate in water, adding sodium dodecylbenzene sulfonate and styrylphenyl polyoxyethylene ether, mixing, adding lentinan and amino-oligosaccharin, and stirring.
Aqueous solution of 1.24% lentinan and amino-oligosaccharin (4: 7)
Lentinan 1.45%
Amino-oligosaccharin 2.55%
2 percent of sodium dodecyl benzene sulfonate
Styrylphenol formaldehyde resin polyoxyethylene polyoxypropylene block polyether 4%
Ethylene glycol 6%
Xanthan gum 0.7%
Supplementing water to 100%
Preparation:
dissolving ethylene glycol in water, adding xanthan gum, sodium dodecyl benzene sulfonate and styrylphenol formaldehyde resin polyoxyethylene polyoxypropylene block polyether, mixing, adding lentinan and amino-oligosaccharin, and stirring.
Aqueous solution of 1.33% lentinan and amino-oligosaccharin (5: 6)
Lentinan 1.36%
Amino-oligosaccharin 1.64%
2 percent of sodium dodecyl benzene sulfonate
Alkylphenol polyoxyethylene ether sulfosuccinate 3%
Glycerol 6 percent
Supplementing water to 100%
Preparation:
dissolving glycerol in water, adding sodium dodecyl benzene sulfonate and alkylphenol polyoxyethylene ether sulfosuccinate, mixing, adding lentinan and amino-oligosaccharin, and stirring.
Aqueous solution of 1.42% lentinan and amino-oligosaccharin (6: 5)
Lentinan 1.09%
0.91 percent of amino-oligosaccharin
Alkylphenol ether sulfosuccinate sodium salt 2%
Polyoxyethylene monostearate 3%
Xanthan gum 1.5%
6 percent of urea
Supplementing water to 100%
Preparation:
dissolving urea in water, adding xanthan gum, sodium alkyl phenol ether sulfosuccinate and polyoxyethylene monostearate, mixing, adding lentinan and amino-oligosaccharin, and stirring.
1.50.5% aqueous solution of lentinan and amino-oligosaccharin (4: 7)
Lentinan 0.18%
0.32 percent of amino-oligosaccharin
Xanthan gum 0.5%
Supplementing water to 100%
Preparation:
dissolving xanthan gum in water, adding lentinan and amino-oligosaccharin, and stirring.
Example 2 indoor bioassay
Refer to Zhao jin et al (feasibility study of screening medicine for treating jujube witches broom in vitro condition [ J)]The tissue culture method of the university of Hebei agriculture, journal 2006,2:9,1: 70-73.) comprises the steps of taking the stem section (with 4 leaves) of a tissue culture seedling of the golden silk jujube with a typical cluster planting symptom to be treated with drugs under the aseptic condition, inoculating the same seedling into MS culture media containing drugs with different doses, wherein the concentration of each drug is 0.1, 1, 2, 5 and 10mg/mL, each treatment is repeated for 3 times, one treatment is carried out in 12 bottles, the illumination is 1400 times and 1600lx, the light period is 16h/8h, the temperature is 25-30 ℃, investigation results are obtained after 30d of culture, and the EC is calculated by adopting SPSS 13.050And a regression equation. Wherein, the co-toxicity coefficient (CTC) is more than or equal to 120, which indicates that the composition has a synergistic effect; 80 < CTC < 120 indicates additive effect; CTC ≤ 80 indicates antagonistic action. The test results are shown in table 1.
TABLE 1 Combined virulence of lentinan and amino-oligosaccharin in different proportions against jujube witches broom pathogen
As can be seen from the data in the table, under indoor conditions, the EC of lentinan and amino-oligosaccharin on the pathogeny of jujube witches broom50The ratio of lentinan to amino-oligosaccharin is respectively 3.904 mg/mL and 1.863 mg/mL, and the ratio is 1:20-20:150Low toxicity, distribution of 1.238-2.978 mg/mL, combined virulence index CTC value of above 120, and synergistic effect, especiallyThe ratio of 1:10-10:1 has more remarkable synergism.
Example 3 field efficacy test
3.1 test agent
Aqueous Solution (AS) of 4% lentinan and amino-oligosaccharin (4: 7): example 1.2;
a 10% tetracycline hydrochloride Aqueous (AS);
20% morpholine guanidine hydrochloride Wettable Powder (WP).
3.2 test article and test site
New No. 4 Ziziphus jujuba, Zhuji Xiang county of Delzhou, Shandong province
3.3 treatment dosage
TABLE 2 dosage of test agents
3.4 cell design
The method is characterized in that the method comprises 30 cells, 20 jujube trees are planted in each cell, the repetition is carried out for 5 times, and the jujube trees are randomly arranged in blocks.
3.5 methods of application
Uniformly spraying the front and back surfaces of the newly germinated jujube trees by using a knapsack electric sprayer, wherein the water consumption is 450kg/hm2And spraying the same amount of clear water on the blank control.
The application times are 3 times, and the application time is 2016, 3 and 10 days, 3 and 15 days, and 3 and 20 days.
And (3) controlling other diseases and insect pests: in 2016, 16 days in 4 months and 15 days in 5 months, 60g/L spinetoram SC and 240g/L methoxyfenozide SC are adopted to prevent and control thrips and date geometrid twice at an interval of 7 days.
3.6 test investigation
The disease incidence condition is investigated 7 days after the second application and 30d and 60d after the third application, and the number of each level of disease plants in all the districts is recorded. The grading standard refers to Xuxiude et al (initial report on the pesticide prevention and treatment technology of jujube witches J Liaoning agricultural science 2004(4): 19-21.) as follows:
level 0: disease free
Level 1: the number of diseased branches is less than 5 percent when the diseased branches are seen at first
And 2, stage: 6 to 10 percent of diseased branches
And 3, level: 11 to 50 percent of diseased branches
4, level: 51-75% of diseased branches
And 5, stage: the number of diseased branches is more than 75 percent
3.7 results and analysis
TABLE 3 respective Agents field control Effect
The data in the table show that the control effect is more than 90% when the contents of the active ingredients of the 4% lentinan and the amino-oligosaccharin AS are 0.05a.i.g/kg and 0.10a.i.g/kg, and although the control effect is slightly higher than 0.05a.i.g/kg when the contents of the active ingredients of the lentinan and the amino-oligosaccharin AS are 0.10a.i.g/kg, no obvious difference exists, and the spraying with the dosage of 0.05a.i.g/kg can be selected for the consideration of production practice and cost. The 10% tetracycline hydrochloride AS and the 20% moroxydine hydrochloride WP show good prevention effect on the jujube witches broom in the initial stage, but have certain inhibition effect on the growth of jujube trees in the later stage, and the jujube witches broom symptoms are repeated.
Example 4 field efficacy test
4.1 test agent
Aqueous Solution (AS) of 4% lentinan and amino-oligosaccharin (4: 7): example 1.2;
0.5% Aqueous Solution (AS) of lentinan and amino-oligosaccharin (4: 7): example 1.5.
4.2 test article and test site
New No. 4 Ziziphus jujuba, Zhuji Xiang county of Delzhou, Shandong province
4.3 treatment dosage
TABLE 4 dosage of test agents
4.4 cell design
The method is characterized in that the method comprises 15 cells in total, 20 jujube trees are planted in each cell, and the repeated operation is carried out for 5 times, and the jujube trees are randomly arranged in blocks.
4.5 application method
4.5.14% of lentinan and amino-oligosaccharin AS
Uniformly spraying the front and back surfaces of the newly germinated jujube trees by using a knapsack electric sprayer, wherein the water consumption is 450kg/hm2And spraying the same amount of clear water on the blank control.
The application times are 3 times, and the application time is 2016, 3 and 10 days, 3 and 15 days, and 3 and 20 days.
4.5.20.5% of lentinan and amino-oligosaccharin AS
The medicine is filled into an infusion bag, one end of the infusion tube is connected into a medicine bottle, the other end of the infusion tube is inserted into the phloem of the jujube tree, and the average medicine amount is 200 mL/day.
4.5.3 other Pest control
In 2016, 16 days in 4 months and 15 days in 5 months, 60g/L spinetoram SC and 240g/L methoxyfenozide SC are adopted to prevent and control thrips and date geometrid twice at an interval of 7 days.
4.6 test investigation
The disease incidence condition is investigated 7 days after the second application and 30d and 60d after the third application, and the number of each level of disease plants in all the districts is recorded. The classification criteria are as follows:
level 0: disease free
Level 1: the number of diseased branches is less than 5 percent when the diseased branches are seen at first
And 2, stage: 6 to 10 percent of diseased branches
And 3, level: 11 to 50 percent of diseased branches
4, level: 51-75% of diseased branches
And 5, stage: the number of diseased branches is more than 75 percent
4.7 results and analysis
TABLE 5 field control of different application modes of the agents
From the data in the table, it can be seen from the field test results that 4% of lentinan and amino-oligosaccharin AS adopt a spray type application method and 0.5% of lentinan and amino-oligosaccharin AS adopt an infusion type application method to prevent and treat jujube witches broom, the prevention effect of the infusion type application method is slightly higher than that of the spray type application method, but there is no obvious difference between the prevention effects of the two application methods, and both application methods can be used in actual application.
Claims (6)
1. An anti-phytoplasma composition containing lentinan is characterized in that active ingredients are lentinan and amino-oligosaccharin; the weight ratio of the lentinan to the amino-oligosaccharin is 3:8-4:7 or 6:5-8: 3.
2. The anti-phytoplasma composition according to claim 1, wherein the total content of the active ingredients lentinan and amino-oligosaccharin is 0.1-20 w.t%, and the balance is auxiliary materials.
3. The anti-phytoplasma composition according to claim 1 or 2, characterised in that the total content of the active ingredients lentinan and amino-oligosaccharin is 0.5-5 w.t%.
4. The anti-phytoplasma composition according to claim 1, wherein the anti-phytoplasma composition is in the form of a soluble powder, a soluble liquid or an aqueous solution.
5. The anti-phytoplasma composition according to claim 4, wherein the dosage form of the anti-phytoplasma composition is an aqueous agent.
6. Use of an anti-phytoplasma composition according to any one of claims 1 to 5 by spraying with water or by injection into the plant delivery system.
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