CN103918681A - Bactericide composition containing thiediazole copper - Google Patents
Bactericide composition containing thiediazole copper Download PDFInfo
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- CN103918681A CN103918681A CN201410174348.2A CN201410174348A CN103918681A CN 103918681 A CN103918681 A CN 103918681A CN 201410174348 A CN201410174348 A CN 201410174348A CN 103918681 A CN103918681 A CN 103918681A
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Abstract
The invention discloses a bactericide composition containing thiediazole copper. The bactericide composition comprises the effective ingredients of thiediazole copper (A) and Zhongshengmycin (B) in the weight part ratio of 50:1 to 1:10. The bactericide composition has obvious synergism, and can be used in control of diseases of vegetables, fruit trees and cereal crops, particularly, of soft rot of Chinese cabbages, bacterial wilt of potatoes, canker of citrus, bacterial blight of rice and bacterial leaf streak of rice.
Description
Technical field
The present invention relates to a kind of bactericidal composition containing Thiodiazole-copper, especially a kind of bactericidal composition that contains Thiodiazole-copper and Zhongshengmycin.
Background technology
Bacterial disease is by the disease due to bacterium infection process, as soft rot, canker, blue or green tangerine disease etc.Bacterial disease is the important disease that affects China's agricultural production, and the bacillary crop pest in the whole world approximately has kind more than 500, and the main bacillary crop pest of China has 60-70 kind.Bacterial disease often causes heavy losses, once outburst, often for want of the agricultural chemicals prevention of efficient, low toxicity, wide spectrum and prevention not in time, cause the vegetables underproduction, and even No kernels or seeds are gathered, as in a year of scarcity for paddy rice.According to estimates, whole world potato is every year because of the bacterial disease underproduction 25%.In order to improve crop yield and quality, it is particularly important that the control of plant disease seems.
Spread use along with Inorganic Copper series bactericidal agents such as Kocide SD, cuprous oxide, basic copper sulfate, copper oxychlorides, the problem that this series bactericidal agent presents is also more and more outstanding: easily produce poisoning, at florescence and young fruit period, ban use of or limit use; Miscibilty is poor, and most of Inorganic Copper preparations are alkaline agricultural chemicals, can not with most of mixed pesticides, use inconvenience; Bring out the propagation of mite class and blister mite, covert increase cost accounting; Market is more random, and profit margin is little, similar copper agent dog-eat-dog; The effect for the treatment of is not strong; On paddy rice, be not easy to use.
Thiodiazole-copper, English common name thiediaZole copper, a kind of thiadiazole bactericide for ZHEJIANG LONGWAN CHEMICALS Co., Ltd.'s research and development, has good preventive effect to bacterial disease.The structure of Thiodiazole-copper is comprised of two groups: the one, and thiazolyl group is efficient therapeutic agent in plant corpus.Medicament is in the pitted vessel of plant, and bacterium is subject to grievous injury, and its cell wall attenuation, disintegrates then, causes the death of bacterium; The 2nd, copper ion, has the effect of not only killing bacterium but also antifungal.Cation on copper ion in medicament and pathogen surface of cell membrane (H+, K+ etc.) exchange, causes the protein coagulating kill pathogens on germ cell membrane; Part copper iontophoretic injection enters in pathogen cell, is combined with some enzyme, affects its activity, causes dysfunction, and germ thereby exhaustion are dead.Under the acting in conjunction of two groups, sterilization is more thorough, and control efficiency is better, and controlling object is more extensive.But use cost is higher separately, exploitation compound synergic composition can improve cost performance.
Different pesticide active ingredients is combined, the obvious formula of synergistic effect can improve preventive effect greatly, reduce dosage, reduce use cost and reduce the pollution to environment, can also delay pathogen and produce resistance, be to solve a kind of quick and effective approach that current single use agricultural chemicals produces resistance and the high problem of drug cost simultaneously.
In sum, the Synergistic bactericidal composition that exploitation contains Thiodiazole-copper is significant.
Summary of the invention
The object of the invention is to: high-efficiency environment friendly, low toxicity, the low-residual of production and application in a kind of applicable agricultural is provided and can delays the bactericidal composition that pathogen produces resistance and reduces agricultural chemicals use cost.
In order to solve the problems of the technologies described above, we have carried out composition research to Thiodiazole-copper.By a large amount of biologicall tests, screen, unexpected discovery Thiodiazole-copper (A) is composite with certain proportion with Zhongshengmycin (B), and the diseases such as soft rot of Chinese cabbage, bacterial wilt of tomato, citrus bacterial canker disease, bacterial blight of rice and bacterial leaf streak of rice are had significant synergies.
Zhongshengmycin, English common name zhongshengmycin, is wide spectrum N-glucoside antibiotic bactericide, can resisting gram-positive, negative bacteria, mycobacterium, saccharomycete and filamentous fungi.Especially to clothing crop pathogenic bacteria as vegetable bacterial soft rot bacterium, avenae subsp.citrull, Xanthomonas oryzae, fusarium graminearums etc. all have obvious antibacterial activity.Zhongshengmycin is due to long-term single continuous use, and product pesticide resistance strengthens, and control efficiency declines, and can solve preferably the deficiency of both single uses with Thiodiazole-copper is composite.
On the basis of above-mentioned discovery, through composition is carried out to symphyogenetic quantitative analysis, formed technical scheme of the present invention, the Thiodiazole-copper (A) of take is active ingredient with Zhongshengmycin (B), the ratio of quality and the number of copies of the two is 50: 1～1: 10, and preferable quality portion rate is 20: 1～1: 5.
The present composition can be prepared into by known method and be applicable to the formulation that agricultural production is used, and in preparation, the total content of active ingredient is 3%～80%.
In composition of the present invention, also comprise that the auxiliary elements such as dispersant, wetting agent, antifreezing agent, defoamer and other are of value to the known substance of active ingredient stable and drug effect performance in storing and using, all various compositions conventional in pesticidal preparations or that allow to use, be not particularly limited, concrete composition and consumption require to determine by simple experiment according to formula.
Composition described in the invention can provide with finished product preparation form, and in composition, each material mixes; The composition of composition also can provide with single dose form, before use, directly in bucket or tank, mixes.
Bactericidal composition of the present invention can be used for preventing and treating various bacterial plant diseases, be specially adapted to prevent and treat the disease of vegetables, fruit tree, cereal crop, as soft rot of Chinese cabbage, bacterial wilt of tomato, citrus bacterial canker disease, bacterial blight of rice and bacterial leaf streak of rice etc.
Compared with prior art, the beneficial effect that the present invention produces is; (1) compare with single dose, this bactericidal composition has obvious synergy to diseases such as soft rot of Chinese cabbage, citrus bacterial canker disease, bacterials blight of rice, has improved control efficiency; (2) can reduce field dosage, effectively reduce residue of pesticide, preserve the ecological environment, reduce use cost; (3) in bactericidal composition, the mechanism of action of active ingredient is different, is conducive to delay the drug-fast generation of germ; (4) can extend medicament retaining the phase in plant corpus, strengthen the premunition of plant.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention is limited to absolutely not these examples.
The present composition be take Thiodiazole-copper (A) as a kind of active ingredient, Zhongshengmycin (B) is another kind of active ingredient, combination between them has obvious synergistic function to diseases such as soft rot of Chinese cabbage, bacterial wilt of tomato, citrus bacterial canker disease, bacterial blight of rice and bacterial leaf streak of rice, and be not only the simple addition of two kinds of pharmacy effects, refer to biologicall test embodiment.
Biologicall test embodiment 1: the composite toxicity test to Chinese cabbage soft rot bacteria of Thiodiazole-copper and Zhongshengmycin
Subjects: Chinese cabbage soft rot bacteria (Erwinia carotovora subsp.carotovora) (pick up from field, and through separation and purification)
Test method: test is with reference to < < pesticide bioassay technology > > (old spring chief editor, publishing house of Beijing Agricultural University publishes), and the agricultural industry criteria NY/T1156.16-2008 < < of People's Republic of China (PRC) farm-chemical indoor determination test rule bactericide the 16th part: bacteria growing inhibiting amount test turbidity method > >, adopt turbidity method to measure Thiodiazole-copper and the indoor virulence of Zhongshengmycin to Chinese cabbage soft rot bacteria.
The cultivation of A, target bacterium: (1L NA medium is containing polyprotein peptone 5g to select NA medium (nutrient agar) medium, dusty yeast 1g, beef extract 3g, sucrose 15g, agar 17g, with the NaOH of 10mol/L, be adjusted to pH7.0) passivation cultivation Chinese cabbage soft rot bacteria 48h in 30 ℃ of bacteriological incubators, picking list bacterium colony, in 30ml NA liquid nutrient medium, is placed in concussion under 28 ℃ of-30 ℃ of conditions and cultivates (120r/min) 18-30h to target bacteria growing to exponential phase.
B, medicament Thiodiazole-copper and Zhongshengmycin are processed and are cultivated: the former medicine of Thiodiazole-copper first dissolves and is mixed with 1% Thiodiazole-copper mother liquor with dimethyl formamide, then dilute with 0.1% the Tween-80 aqueous solution; The female medicine of Zhongshengmycin is directly dissolved as 10 with distilled water
4μ g/ml mother liquor, arranges 5～7 series mass concentration gradients, by the concentration quantitative of design, adds medicament in 30ml NA liquid nutrient medium, and every processing arranges 4 repetitions, and does not establish and make blank (CK) containing the processing of active ingredient.To each, process the target bacterium 100ul bacterium liquid of inoculating respectively exponential phase in medium, be placed under 28 ℃ of-30 ℃ of conditions concussion and cultivate (120r/min) 16-20h when the contrast bacteria growing of adding medicine does not reach exponential phase, measures and records the respectively turbidity of processing.
The mensuration of turbidity: the bacterium before measuring chemicals treatment and after processing is at the absorbance value of 600nm.
Computational methods:
With DPS data processing software, carry out statistical analysis, calculate the EC of each medicament
50, control efficiency is converted into probability value (y), liquor strength (ug/ml) converts logarithm value (x) to, with method of least squares, calculates concentration EC in virulence equation and inhibition
50, then press toxicity index and co-toxicity coefficient (CTC) that the abundant method of Sun Yun is calculated medicament.Toxicity test the results are shown in Table 1.
Actual measurement toxicity index (ATI)=(standard medicament EC
50/ reagent agent EC
50) * 100
The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] * 100
When CTC≤80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance is for summation action, and when CTC >=120, composition performance is synergistic effect.
The composite Toxicity Determination result to Chinese cabbage soft rot bacteria of table 1 Thiodiazole-copper and Zhongshengmycin
In table 1, result shows, Thiodiazole-copper and Zhongshengmycin, proportioning 50: 1～1: 10 o'clock, show as synergistic effect to Chinese cabbage soft rot bacteria, and especially, at 20: 1～1: 5 o'clock, synergistic effect is more obvious, and co-toxicity coefficient is all more than 183.
Biologicall test embodiment 2: the composite toxicity test to c itrus canker germ of Thiodiazole-copper and Zhongshengmycin
Subjects: c itrus canker germ (Xanthomonas citri (Hahse)) (pick up from field, and through separation and purification)
Test method: test is with reference to < < pesticide bioassay technology > > (old spring chief editor, publishing house of Beijing Agricultural University publishes), and the agricultural industry criteria NY/T1156.16-2008 < < of People's Republic of China (PRC) farm-chemical indoor determination test rule bactericide the 16th part: bacteria growing inhibiting amount test turbidity method > >, adopt turbidity method to measure Thiodiazole-copper and the indoor virulence of Zhongshengmycin to c itrus canker germ.
The cultivation of A, target bacterium: (1LNA medium is containing polyprotein peptone 5g to select NA medium (nutrient agar) medium, dusty yeast 1g, beef extract 3g, sucrose 15g, agar 17g, with the NaOH of 10mol/L, be adjusted to pH7.0) passivation cultivation Chinese cabbage soft rot bacteria 48h in 30 ℃ of bacteriological incubators, picking list bacterium colony, in 30ml NA liquid nutrient medium, is placed in concussion under 28 ℃ of-30 ℃ of conditions and cultivates (120r/min) 18-30h to target bacteria growing to exponential phase.
B, medicament Thiodiazole-copper and Zhongshengmycin are processed and are cultivated: the former medicine of Thiodiazole-copper first dissolves and is mixed with 1% Thiodiazole-copper mother liquor with dimethyl formamide, then dilute with 0.1% the Tween-80 aqueous solution; The female medicine of Zhongshengmycin is directly dissolved as 10 with distilled water
4μ g/ml mother liquor, arranges 5～7 series mass concentration gradients, by the concentration quantitative of design, adds medicament in 30ml NA liquid nutrient medium, and every processing arranges 4 repetitions, and does not establish and make blank (CK) containing the processing of active ingredient.To each, process the target bacterium 100ul bacterium liquid of inoculating respectively exponential phase in medium, be placed under 28 ℃ of-30 ℃ of conditions concussion and cultivate (120r/min) 16-20h when the contrast bacteria growing of adding medicine does not reach exponential phase, measures and records the respectively turbidity of processing.
The mensuration of turbidity: the bacterium before measuring chemicals treatment and after processing is at the absorbance value of 600nm.
Computational methods:
With DPS data processing software, carry out statistical analysis, calculate the EC of each medicament
50, control efficiency is converted into probability value (y), liquor strength (μ g/ml) converts logarithm value (x) to, with method of least squares, calculates concentration EC in virulence equation and inhibition
50, then press toxicity index and co-toxicity coefficient (CTC) that the abundant method of Sun Yun is calculated medicament.Toxicity test the results are shown in Table 2.
Actual measurement toxicity index (ATI)=(standard medicament EC
50/ reagent agent EC
50) * 100
The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] * 100
When CTC≤80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance is for summation action, and when CTC >=120, composition performance is synergistic effect.
The composite Toxicity Determination result to c itrus canker germ of table 2 Thiodiazole-copper and Zhongshengmycin
| Process | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Thiodiazole-copper | 25.742 | 100.00 | / | / |
| Zhongshengmycin | 1.725 | 1492.29 | / | / |
| Thiodiazole-copper: Zhongshengmycin=50: 1 | 15.564 | 165.39 | 127.30 | 129.93 |
| Thiodiazole-copper: Zhongshengmycin=20: 1 | 8.396 | 306.60 | 166.30 | 184.37 |
| Thiodiazole-copper: Zhongshengmycin=10: 1 | 5.735 | 448.86 | 226.57 | 198.11 |
| Thiodiazole-copper: Zhongshengmycin=5: 1 | 3.852 | 668.28 | 332.05 | 201.26 |
| Thiodiazole-copper: Zhongshengmycin=1: 1 | 2.013 | 1278.79 | 796.14 | 160.62 |
| Thiodiazole-copper: Zhongshengmycin=1: 5 | 1.057 | 2435.38 | 1260.24 | 193.25 |
| Thiodiazole-copper: Zhongshengmycin=1: 10 | 1.432 | 1797.63 | 1365.72 | 131.62 |
In table 2, result shows, Thiodiazole-copper and Zhongshengmycin, proportioning 50: 1～1: 10 o'clock, show as synergistic effect to c itrus canker germ, and in the time of especially between 20: 1～1: 5, listed proportioning co-toxicity coefficient is all higher than 160, and synergistic effect is more obvious.
Biologicall test embodiment 3: the composite toxicity test to Xanthomonas oryzae of Thiodiazole-copper and Zhongshengmycin
Subjects: Xanthomonas oryzae (Xanthomonas.oryza) (pick up from field, and through separation and purification)
Test method: test is with reference to < < pesticide bioassay technology > > (old spring chief editor, publishing house of Beijing Agricultural University publishes), and the agricultural industry criteria NY/T1156.16-2008 < < of People's Republic of China (PRC) farm-chemical indoor determination test rule bactericide the 16th part: bacteria growing inhibiting amount test turbidity method > >, adopt turbidity method to measure Thiodiazole-copper and the indoor virulence of Zhongshengmycin to Xanthomonas oryzae.
The cultivation of A, target bacterium: (1LNA medium is containing polyprotein peptone 5g to select NA medium (nutrient agar) medium, dusty yeast 1g, beef extract 3g, sucrose 15g, agar 17g, with the NaOH of 10mol/L, be adjusted to pH7.0) passivation cultivation Chinese cabbage soft rot bacteria 48h in 30 ℃ of bacteriological incubators, picking list bacterium colony, in 30ml NA liquid nutrient medium, is placed in concussion under 28 ℃ of-30 ℃ of conditions and cultivates (120r/min) 18-30h to target bacteria growing to exponential phase.
B, medicament Thiodiazole-copper and Zhongshengmycin are processed and are cultivated: the former medicine of Thiodiazole-copper first dissolves and is mixed with 1% Thiodiazole-copper mother liquor with dimethyl formamide, then dilute with 0.1% the Tween-80 aqueous solution; The female medicine of Zhongshengmycin is directly dissolved as 10 with distilled water
4μ g/ml mother liquor, arranges 5～7 series mass concentration gradients, by the concentration quantitative of design, adds medicament in 30ml NA liquid nutrient medium, and every processing arranges 4 repetitions, and does not establish and make blank (CK) containing the processing of active ingredient.To each, process the target bacterium 100ul bacterium liquid of inoculating respectively exponential phase in medium, be placed under 28 ℃ of-30 ℃ of conditions concussion and cultivate (120r/min) 16-20h when the contrast bacteria growing of adding medicine does not reach exponential phase, measures and records the respectively turbidity of processing.
The mensuration of turbidity: the bacterium before measuring chemicals treatment and after processing is at the absorbance value of 600nm.
Computational methods:
With DPS data processing software, carry out statistical analysis, calculate the EC of each medicament
50, control efficiency is converted into probability value (y), liquor strength (μ g/ml) converts logarithm value (x) to, with method of least squares, calculates concentration EC in virulence equation and inhibition
50, then press toxicity index and co-toxicity coefficient (CTC) that the abundant method of Sun Yun is calculated medicament.Toxicity test the results are shown in Table 3.
Actual measurement toxicity index (ATI)=(standard medicament EC
50/ reagent agent EC
50) * 100
The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] * 100
When CTC≤80, composition performance, for antagonism, is worked as 80<CTC<120, and composition performance is for summation action, and when CTC >=120, composition performance is synergistic effect.
The composite Toxicity Determination result to Xanthomonas oryzae of table 3 Thiodiazole-copper and Zhongshengmycin
| Process | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Thiodiazole-copper | 2.632 | 100.00 | / | / |
| Zhongshengmycin | 0.261 | 1008.43 | / | / |
| Thiodiazole-copper: Zhongshengmycin=50: 1 | 1.764 | 149.21 | 117.81 | 126.65 |
| Thiodiazole-copper: Zhongshengmycin=20: 1 | 0.898 | 293.10 | 143.26 | 204.59 |
| Thiodiazole-copper: Zhongshengmycin=10: 1 | 0.756 | 348.15 | 182.58 | 190.68 |
| Thiodiazole-copper: Zhongshengmycin=5: 1 | 0.528 | 498.48 | 251.40 | 198.28 |
| Thiodiazole-copper: Zhongshengmycin=1: 1 | 0.274 | 960.58 | 554.21 | 173.32 |
| Thiodiazole-copper: Zhongshengmycin=1: 5 | 0.184 | 1430.43 | 857.02 | 166.91 |
| Thiodiazole-copper: Zhongshengmycin=1: 10 | 0.223 | 1180.27 | 925.84 | 127.48 |
In table 3, result shows, Thiodiazole-copper and Zhongshengmycin, proportioning 50: 1～1: 10 o'clock, all show as synergistic effect to Xanthomonas oryzae, and in the time of especially between 20: 1～1: 5, listed proportioning co-toxicity coefficient is all higher than 166, and synergistic effect is very obvious.
Bactericidal composition of the present invention can be prepared into by known method suspending agent, wetting powder, water dispersible granules or the aqua that is applicable to agricultural use.With specific embodiment, describe below, in present specification, active component or active ingredient refer to Thiodiazole-copper and Zhongshengmycin, and in formula, percentage is mass percent, below repeats no more.
Embodiment 1:25.5% Thiodiazole-copper Zhongshengmycin suspending agent
Each components such as Thiodiazole-copper and the former medicine of Zhongshengmycin and dispersant, wetting agent, defoamer, antifreezing agent, thickener, preservative and water are mixed by formula rate, after sand milling and high speed shear, reach certain index, be deployed into 25.5% Thiodiazole-copper Zhongshengmycin suspending agent.
This embodiment is applied to prevent and treat soft rot of Chinese cabbage.25.5% Thiodiazole-copper Zhongshengmycin suspending agent is pressed to 230 grams/ha of effective ingredient consumption, at soft rot of Chinese cabbage their early stage, Chinese cabbage complete stool is carried out to even spraying, take squirt blade do not drip as degree, every about 7 days, spray once, spray altogether 3 times, after last medicine, the control efficiency of 7 days and 14 days is respectively 88.2% and 86.4%.The effective ingredient consumption of contrast medicament 20% Thiodiazole-copper suspending agent and 3% Zhongshengmycin wetting powder is respectively 300 grams/ha and 40 grams/ha, adopts identical application method, and after last medicine, the preventive effect of 7 days is respectively 78.6% and 70.9%; After last medicine, the preventive effect of 14 days is respectively 76.4% and 68.2%.Thiodiazole-copper and Zhongshengmycin composite rear to the preventive effect of soft rot of Chinese cabbage significantly better than contrasting single dose.
Embodiment 2:42% Thiodiazole-copper Zhongshengmycin wetting powder
After Thiodiazole-copper and the former medicine of Zhongshengmycin and wetting agent, dispersant, diffusant and filler etc. are mixed successively, pulverize, obtain 42% Thiodiazole-copper Zhongshengmycin wetting powder.
This embodiment is applied to prevent and treat oranges and tangerines (fruit) canker.42% Thiodiazole-copper Zhongshengmycin wetting powder is pressed to effective ingredient consumption 210 mg/kg (diluting 2000 times), at citrus bacterial canker disease their early stage, oranges and tangerines complete stool is carried out to even spraying, take squirt blade do not drip as degree, every 7-10 days, spray once, spray altogether 3 times, after last medicine, the control efficiency of 15 days is 87.3%.Contrast medicament 20% Thiodiazole-copper suspending agent and 3% Zhongshengmycin wetting powder effective ingredient consumption are respectively by 400 mg/kg (diluting 500 times) and 37.5 mg/kg (diluting 800 times), adopt identical application method, after last medicine, the preventive effect of 15 days is respectively 76.7% and 69.8%.Thiodiazole-copper and Zhongshengmycin composite rear to the preventive effect of citrus bacterial canker disease significantly better than contrasting single dose.
Embodiment 3:80% Thiodiazole-copper Zhongshengmycin water dispersible granules
Thiodiazole-copper and the former medicine of Zhongshengmycin and wetting agent, dispersant, diffusant and filler are mixed, through coarse crushing and air-flow crushing, then mediate and extruder grain, dryly by screening, make 80% Thiodiazole-copper Zhongshengmycin water dispersible granules.
This embodiment is applied to bacterial blight of rice.80% Thiodiazole-copper Zhongshengmycin water dispersible granules is pressed to 240 grams/ha of effective ingredient consumption, at bacterial blight of rice their early stage, paddy rice complete stool is carried out to even spraying, take squirt blade do not drip as degree, every about 10 days, spray once, spray altogether 3 times, after last medicine, the control efficiency of 14 days is 90.6%.The effective ingredient consumption of contrast medicament 20% Thiodiazole-copper suspending agent and 3% Zhongshengmycin wetting powder is respectively 360 grams/ha and 81 grams/ha, adopts identical application method, and after last medicine, the preventive effect of 14 days is respectively 81.3% and 72.8%.Thiodiazole-copper and Zhongshengmycin composite rear to the preventive effect of bacterial blight of rice significantly better than contrasting single dose.
Embodiment 4:3% Thiodiazole-copper Zhongshengmycin aqua
Auxiliary agent, cosolvent and the water such as Thiodiazole-copper and the former medicine of Zhongshengmycin and wetting agent, emulsifier are added by certain processing sequence and stirred, be deployed into 3% Thiodiazole-copper Zhongshengmycin aqua.
This embodiment is applied to bacterial wilt of tomato.3% Thiodiazole-copper Zhongshengmycin aqua is pressed to effective ingredient consumption 42.86 mg/kg (diluting 700 times), at bacterial wilt of tomato their early stage, start to fill with root, every 7-10 days, fill with once, continuous use 2-3 time, after last medicine, the control efficiency of 14 days reaches 92.4%.The effective ingredient consumption of contrast medicament 20% Thiodiazole-copper suspending agent and 3% Zhongshengmycin wetting powder is respectively 400 mg/kg (diluting 500 times) and 50 mg/kg (diluting 600 times), adopt identical application method, after last medicine, the preventive effect of 14 days is respectively 83.6% and 78.2%.Thiodiazole-copper and Zhongshengmycin composite rear to the preventive effect of bacterial wilt of tomato significantly better than contrasting single dose.
Embodiment 5:5.5% Thiodiazole-copper Zhongshengmycin wetting powder
After Thiodiazole-copper and the former medicine of Zhongshengmycin and wetting agent, dispersant, diffusant and filler etc. are mixed successively, pulverize, obtain 5.5% Thiodiazole-copper Zhongshengmycin wetting powder.
This embodiment is applied to prevent and treat bacterial leaf streak of rice.5.5% Thiodiazole-copper Zhongshengmycin wetting powder is pressed to 66 grams/ha of effective ingredient consumption, at bacterial leaf streak of rice their early stage, paddy rice complete stool is carried out to even spraying, take squirt blade do not drip as degree, every 7-10 days, spray once, spray altogether 3 times, after last medicine, the control efficiency of 15 days is 86.7%.Contrast medicament 20% Thiodiazole-copper suspending agent and 3% Zhongshengmycin wetting powder effective ingredient consumption by 480 grams/ha and 81 grams/ha, adopt identical application method respectively, and after last medicine, the preventive effect of 15 days is respectively 75.6% and 68.3%.Thiodiazole-copper and Zhongshengmycin composite rear to the preventive effect of bacterial leaf streak of rice significantly better than contrasting single dose.
Claims (5)
1. containing a bactericidal composition for Thiodiazole-copper, it is characterized in that: in described bactericidal composition, active ingredient is Thiodiazole-copper (A) and Zhongshengmycin (B), is (A) 50: 1～1: 10 with ratio of quality and the number of copies (B).
2. bactericidal composition according to claim 1, is characterized in that: active ingredient (A) is 20: 1～1: 5 with ratio of quality and the number of copies (B).
3. according to bactericidal composition described in claim 1 or 2, it is characterized in that: active ingredient (A) is 3%～80% with gross mass percentage composition (B).
4. bactericidal composition according to claim 3, is characterized in that: the formulation of described bactericidal composition is suspending agent, wetting powder, water dispersible granules or aqua.
5. bactericidal composition according to claim 1 is for preventing and treating the purposes of vegetables, fruit tree, cereal crop disease.
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| CN104222108A (en) * | 2014-09-02 | 2014-12-24 | 浙江龙湾化工有限公司 | Compound fungicide containing thiediazole copper |
| CN104255716A (en) * | 2014-09-02 | 2015-01-07 | 浙江龙湾化工有限公司 | Thiediazole copper water dispersible granule and preparation method thereof |
| CN104472557A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Brazil cherry seed treatment agent for control of large cherry foxiness shothole disease |
| CN105941482A (en) * | 2016-05-25 | 2016-09-21 | 中国水稻研究所 | Compound pesticide containing Zhongshengmycin and Saisentong and application of compound pesticide |
| CN106234382A (en) * | 2016-08-23 | 2016-12-21 | 浙江龙湾化工有限公司 | A kind of containing Thiodiazole-copper and the bactericidal composition of shenqinmycin and antibacterial |
| CN106689202A (en) * | 2015-07-20 | 2017-05-24 | 江苏龙灯化学有限公司 | Bactericidal composition |
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| CN104222108A (en) * | 2014-09-02 | 2014-12-24 | 浙江龙湾化工有限公司 | Compound fungicide containing thiediazole copper |
| CN104255716A (en) * | 2014-09-02 | 2015-01-07 | 浙江龙湾化工有限公司 | Thiediazole copper water dispersible granule and preparation method thereof |
| CN104222108B (en) * | 2014-09-02 | 2016-08-24 | 浙江龙湾化工有限公司 | A kind of compound disinfectant containing Thiodiazole-copper |
| CN104472557A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Brazil cherry seed treatment agent for control of large cherry foxiness shothole disease |
| CN106689202A (en) * | 2015-07-20 | 2017-05-24 | 江苏龙灯化学有限公司 | Bactericidal composition |
| CN106689202B (en) * | 2015-07-20 | 2019-11-22 | 江苏龙灯化学有限公司 | A kind of bactericidal composition |
| CN105941482A (en) * | 2016-05-25 | 2016-09-21 | 中国水稻研究所 | Compound pesticide containing Zhongshengmycin and Saisentong and application of compound pesticide |
| CN106234382A (en) * | 2016-08-23 | 2016-12-21 | 浙江龙湾化工有限公司 | A kind of containing Thiodiazole-copper and the bactericidal composition of shenqinmycin and antibacterial |
| CN107821407A (en) * | 2017-11-29 | 2018-03-23 | 孙铭岐 | A kind of bactericide for preventing and treating Characters of Erwinia chrysanthemi pv. zeae |
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