CN106172424A - A kind of containing amisulbrom and the bactericidal composition of amino-oligosaccharide and application - Google Patents
A kind of containing amisulbrom and the bactericidal composition of amino-oligosaccharide and application Download PDFInfo
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- CN106172424A CN106172424A CN201610581919.3A CN201610581919A CN106172424A CN 106172424 A CN106172424 A CN 106172424A CN 201610581919 A CN201610581919 A CN 201610581919A CN 106172424 A CN106172424 A CN 106172424A
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- oligosaccharide
- amisulbrom
- amino
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- bactericidal composition
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Abstract
The invention provides a kind of containing amisulbrom and the bactericidal composition of amino-oligosaccharide and application, said composition includes effective ingredient amisulbrom and effective ingredient amino-oligosaccharide, both mass ratioes are 100:1 ~ 1:100, and in its bactericidal composition, the gross mass percentage composition of effective ingredient is 5% ~ 85%.Bactericidal composition of the present invention can be made into suspending agent, suspension emulsion, microemulsion, aqueous emulsion, microcapsule suspending agent, microcapsule suspension suspending agent, wettable powder, water dispersible granules.Bactericidal composition of the present invention has significant potentiation synergism, it is possible to reduces Pesticide use amount, improve prevention effect, low toxicity and low residue, safety and environmental protection, environmentally friendly, to crop safety.The present invention is for preventing and treating the diseases such as downy mildew on vegetable, fruit tree, Nicotiana tabacum L., early blight, late blight, balck shank.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of containing effective ingredient amisulbrom and effective ingredient ammonia
The bactericidal composition of base oligosaccharide and the application of preventing and treating corps diseases thereof.
Background technology
Amisulbrom, english common name is misulrom, chemical name be 3-(the fluoro-2 methyl indole of the bromo-6-of 3--
1-sulfonyl)-N, N-dimethyl-1,2,4-triazole-1-sulfonamide, molecular formula is C13H13rFN5O4S2.This medicament is a kind of knot
The triazolylsulfonamide compounds that structure novelty is unique, belongs to contact killing type antibacterial.Mainly effective to oomycetes and slime molds, pass through
It is attached to the Qi site on cytochrome complex c1, thus suppresses fungus to breathe and the sprouting of spore；To fruit tree, vegetable, flower
The epidemic disease of grass etc., downy mildew and black shank have higher activity, and lasting effect is strong, resistance to rainwater shower.
Amino-oligosaccharide, the most agriculture special oligochitosan.Refer to what D-glucosamine connected with β-1.4 glycosidic bond
Oligosaccharide, chitin degrading after obtaining chitosan, degraded prepares again, or the low toxicity antibacterial extracted by fermentable.To some
The growth of pathogenic bacteria produces inhibitory action, affect fungus spore germination, induces that hypha form morphs, biochemistry changes in spore
Deng.Gene in plant can be excited, produce and there are the chitinase of resistant effect, glucanase, guarantor's element and PR albumen etc., and have
Cell activation effect, the recovery of the plant that contributes to being injured, promote root strong sprout, to strengthen the resistance of crop, promote growth and development of plants.
The diseases such as downy mildew of garpe, tomato late blight, black shank, anthrax, gray mold had good preventive and therapeutic effect.
At present in more fungus-caused plant diseases, particularly downy mildew, early blight, late blight, clubroot and
Balck shank, the use of a large amount of chemical agents, the most substantially produce resistance；And prevented and treated by biological pesticide, its prevention effect also has
Wait to improve.At present the active component of chemical pesticide and biological pesticide is compounded, on the one hand can effectively delay Disease Resistance
Generation, hence it is evident that improve preventive effect；On the other hand it is substantially reduced the usage amount of pesticide, reduces use cost, the most also can expand and kill
Bacterium is composed, and improves germicidal efficiency.
Inventor passes through a large amount of indoor biometricses and field control effectiveness test, finds that amisulbrom and amino-oligosaccharide are with necessarily
Using after ratio mixture, preventive effect improves, the lasting period extends, and dosage reduces, use cost reduces, and toxicity reduces, residual quantity subtracts
Few, ensure food safety and Environmental security.It is the strongest that lot of experiments confirms that amisulbrom and amino-oligosaccharide have after compounding
Potentiation, and both researchs of compounding, up to now, there is not yet relevant report.
Summary of the invention
It is an object of the invention to, it is provided that one delaying drug resistance can produce, reduce dosage, environmentally safe, preventive effect
The good bactericidal composition containing amisulbrom Yu amino-oligosaccharide.
The technical scheme is that
It is a kind of containing amisulbrom and the bactericidal composition of amino-oligosaccharide and application, it is characterised in that: described sterilization group
Compound contains effective ingredient amisulbrom and effective ingredient amino-oligosaccharide, and both mass ratioes are 100:1 ~ 1:100.
Described bactericidal composition, it is characterised in that: effective ingredient amisulbrom and effective ingredient amino-oligosaccharide, two
The mass ratio of person is preferably 50:1 ~ 1:50.
Described bactericidal composition, it is characterised in that: effective ingredient amisulbrom and effective ingredient amino-oligosaccharide, two
The mass ratio of person is preferably 20:1 ~ 1:20.
Described bactericidal composition, it is characterised in that: in bactericidal composition the gross mass percentage composition of effective ingredient be 5% ~
85%。
The mass ratio that amisulbrom and amino-oligosaccharide are formed is 100:1 ~ 1:100.Generally compositions effectively becomes
The gross mass percentage composition divided is 2% ~ 90%, is preferably 5% ~ 85%.According to different preparation types, active constituent content scope
Different.Generally, the gross mass of solid preparation effective ingredient is 5% ~ 90%, is preferably 10% ~ 85%；Liquid preparation effectively becomes
The gross mass divided is 1% ~ 70%, is preferably 5% ~ 50%.
Bactericidal composition of the present invention, can be processed into any dosage form agriculturally allowed, the most more preferably dosage form
There are suspending agent, suspension emulsion, microemulsion, aqueous emulsion, microcapsule suspending agent, microcapsule suspension-suspendinagent agent, wettable powder, water-dispersible grain
Agent.
When above-described bactericidal composition is suspending agent, preparation comprises following component: amisulbrom 0.1% ~ 50%,
Amino-oligosaccharide 0.1% ~ 50%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, defoamer 0.1% ~ 1%, thickening agent 0.1% ~ 2%, antifreeze
Agent 0.1% ~ 8%, deionized water add to 100%.
This concrete procedure of processing of bactericidal composition suspending agent is: according to above-mentioned formula by dispersant, wetting agent, defoamer,
Thickening agent, antifreezing agent, through high speed shear mix homogeneously, add effective ingredient amisulbrom, amino-oligosaccharide, at ball milling
Ball milling 2 ~ 3 hours in machine, make diameter of particle all below 5 μm, i.e. can be made into suspending agent of the present invention.
When above-described bactericidal composition is suspension emulsion, preparation comprises following component: amisulbrom 0.1% ~ 50%,
Amino-oligosaccharide 0.1% ~ 50%, emulsifying agent 2% ~ 12%, dispersant 2% ~ 10%, defoamer 0.1% ~ 2%, thickening agent 0.1% ~ 2%, antifreeze
Agent 0.1% ~ 8%, preservative 0.05% ~ 3%, deionized water add to 100%.
This concrete procedure of processing of bactericidal composition suspension emulsion is: according to above-mentioned formula by dispersant, defoamer, thickening agent,
Antifreezing agent, preservative add amino-oligosaccharide through high speed shear mix homogeneously, and in ball mill, ball milling 2 ~ 3 hours, make microgranule
Particle diameter, all below 5 μm, prepares amino-oligosaccharide suspending agent, then that amisulbrom, emulsifying agent and various auxiliary agent is high
The speed direct emulsifying of agitator, in suspending agent, i.e. can be made into suspension emulsion of the present invention.
When above-described bactericidal composition is microemulsion, preparation comprises following component: amisulbrom 0.1% ~ 50%,
Amino-oligosaccharide 0.1% ~ 50%, solvent 1% ~ 30%, emulsifying agent 3% ~ 20%, antifreezing agent 0.1% ~ 10%, thickening agent 0.1% ~ 2%, froth breaking
Agent 0.01% ~ 2%, preservative 0.05% ~ 4%, deionized water add to 100%.
This concrete procedure of processing of bactericidal composition microemulsion is: according to above-mentioned formula by amisulbrom and amino-oligosaccharide
It is dissolved in the homogenizer equipped with solvent and cosolvent, emulsifying agent, preservative, antifreezing agent and water are joined equipped with above-mentioned solution
Homogenizer in, strongly mix and homogenize, finally preparing the microemulsion that clear appearance is transparent, being of the present invention micro-
Emulsion.
When above-described bactericidal composition is aqueous emulsion, preparation comprises following component: amisulbrom 0.1% ~ 50%,
Amino-oligosaccharide 0.1% ~ 50%, solvent 1% ~ 30%, emulsifying agent 1% ~ 15%, antifreezing agent 0.1% ~ 10%, thickening agent 0.1% ~ 2%, froth breaking
Agent 0.01% ~ 2%, deionized water add to 100%.
This concrete procedure of processing of bactericidal composition aqueous emulsion is: according to above-mentioned formula by effective ingredient amisulbrom, ammonia
Base oligosaccharide, solvent, emulsifying agent are added together, and make to be dissolved into homogeneous oil phase；Part water, antifreezing agent, defoamer are blended in one
Play uniformly aqueous phase；While high-speed stirred, oil phase is added aqueous phase in a kettle., adds thickening agent, slowly add water until
Reach special phase point, open cutter and carry out high speed shear, and add remaining water, shear about half an hour, form oil-in-water type
Aqueous emulsion.It is aqueous emulsion of the present invention.
When above-described bactericidal composition is microcapsule suspending agent, preparation comprises following component: amisulbrom 0.1% ~
50%, amino-oligosaccharide 0.1% ~ 50%, encystation monomer 1% ~ 10%, dispersant 2% ~ 10%, solvent 1% ~ 10%, emulsifying agent 2% ~ 7%, anti-
Freeze agent 0.1% ~ 10%, thickening agent 0.1% ~ 2%, preservative 0.05% ~ 4%, pH adjusting agent 2% ~ 5%, deionized water add to 100%.
This concrete procedure of processing of bactericidal composition microcapsule suspending agent is: according to above-mentioned formula by effective ingredient indazole sulphur bacterium
Amine, amino-oligosaccharide, encystation monomer, solvent mix, and make to be dissolved into homogeneous oil phase, in a shear condition, are joined by oil phase and contain
Having in the aqueous phase solution of emulsifying agent, pH adjusting agent, dispersant, bi-material reacts at oil-water interfaces, forms high score ascus
Wall, i.e. can be made into microcapsule suspending agent of the present invention.
When above-described bactericidal composition is microcapsule suspension-suspendinagent agent, preparation comprises following component: amisulbrom
0.1% ~ 50%, amino-oligosaccharide 0.1% ~ 50%, encystation monomer 1% ~ 12%, wetting agent 2% ~ 15%, solvent 1% ~ 15%, emulsifying agent 1% ~
8%, defoamer 0.2% ~ 5%, thickening agent 0.2% ~ 5%, antifreezing agent 0.1% ~ 10%, pH adjusting agent 0.1% ~ 5%, deionized water adds to
100%。
This concrete procedure of processing of bactericidal composition microcapsule suspension-suspendinagent agent is: according to above-mentioned formula by effective ingredient indazole
Flusulfamide, encystation monomer, solvent mix, make to be dissolved into homogeneous oil phase, oil phase is joined in a shear condition containing emulsifying agent,
In the aqueous phase solution of pH adjusting agent, bi-material reacts at oil-water interfaces, high around the droplet formation containing chemical substance
Molecule cyst wall, makes finely disseminated microcapsule suspending agent.Wetting agent, defoamer, thickening agent, antifreezing agent are mixed through high speed shear
Close uniformly, add amino-oligosaccharide, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μm, prepare and suspend
Agent.Then, suspending agent is joined in the aqueous phase solution of micro-capsule suspension, i.e. can be made into microcapsule suspension of the present invention-
Suspending agent.
When above-described bactericidal composition is wettable powder, preparation comprises following component: amisulbrom 1% ~
80%, amino-oligosaccharide 1% ~ 80%, dispersant 2% ~ 10%, wetting agent 2% ~ 8%, filler supplement surplus.
This concrete procedure of processing of bactericidal composition wettable powder is: according to above-mentioned formula by effective ingredient indazole sulphur bacterium
Amine, amino-oligosaccharide, dispersant, wetting agent, filler mix, mix homogeneously in mixed cylinder, after jet mill is pulverized again
Mix homogeneously, i.e. can be made into wettable powder of the present invention.
When above-described bactericidal composition is water dispersible granules, preparation comprises following component: amisulbrom 1% ~
80%, amino-oligosaccharide 1% ~ 80%, dispersant 3% ~ 9%, wetting agent 1% ~ 8%, disintegrating agent 1% ~ 10%, filler supplement surplus.
This concrete procedure of processing of bactericidal composition water dispersible granules is: according to above-mentioned formula by effective ingredient indazole sulphur bacterium
Amine, amino-oligosaccharide, dispersant, wetting agent, disintegrating agent, filler mix homogeneously, pulverize with micro jet, through mediating,
Be subsequently adding in fluidized-bed granulation dryer carry out pelletize, be dried, screening after, i.e. can be made into water-dispersible grain of the present invention
Agent.
Described solvent is selected from: DMF, Ketohexamethylene, methanol, ethanol, 3-methyl cyclohexanol ketenes, N-are pungent
Base ketopyrrolidine, N-ketopyrrolidine, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol monomethyl ether, butyl ether, ethyl acetate, plant
One or more in oil, acetonitrile.
Described cosolvent is selected from: one or more in dimethylformamide, isopropanol, the third ethanol, acetone.
Described emulsifying agent is selected from: alkyl benzene sulfonate calcium salt, styrene polyoxyethylene ether ammonium sulfate salt, alkyl biphenyl ether two
Sulfonic acid magnesium salt, triethanolamine salt, phenethyl phenol polyethenoxy ether, 506#, 600#, 700#, NP-10 phosphate ester, OP-10 phosphoric acid
One or more in ester, castor oil polyoxyethylene ether, sorbester p17, Tween 80,602#.
Described dispersant is selected from: alkylnaphthalene sulfonate, double (alkyl) naphthalenesulfonate formaldehyde condensation compound, naphthalenesulfonateformaldehyde formaldehyde contract
Compound, aryl phenol polyoxyethylene succinate sulfonate, octylphenol polyethylene oxyethylene group ether sulfate, polycarboxylate, sulfomethylated lignin
Hydrochlorate, to methoxyl group fatty acyl anilinesulfonic acid, alkylphenol polyoxyethylene formaldehyde condensation products sulfate, alkyl benzene calcium sulfonate
Salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, ester
One or more in polyoxyethylene ether.
Described wetting agent is selected from: sodium lauryl sulphate, dodecylbenzene sodium sulfonate, pull open powder X, wetting and penetrating agent F,
One or more in spaonin powder, Bombyx mori L., Fructus Sapindi Mukouossi powder.
Described disintegrating agent is selected from: in bentonite, ammonium sulfate, citric acid, carbamide, aluminum chloride, succinic acid, sodium bicarbonate
One or more.
Described encystation monomer is selected from: toluene di-isocyanate(TDI), methyl diphenylene diisocyanate, polymethylene polyphenyl
One or more in polyisocyanates, polyether polyol.
Described antifreezing agent is selected from: one or more in ethylene glycol, propylene glycol, glycerol, Polyethylene Glycol.
Described defoamer is selected from: C8~10Aliphatic alcohols, C10~20Saturated fat acids (such as capric acid) and amide, silicone oil, have
One or more in machine silicone compound.
Described thickening agent is selected from: xanthan gum, hydroxymethyl cellulose, hydroxyethyl cellulose, methylcellulose, magnesium silicate
One or more in aluminum, polyvinyl alcohol.
Described preservative is selected from: sodium benzoate, sodium propionate, butyl p-hydroxybenzoate, dimethyl fumarate, sorbic acid
One or more in potassium, sodium diacetate.
Described filler is selected from: in Kaolin, kieselguhr, bentonite, attapulgite, white carbon, starch, precipitated calcium carbonate
One or more.
The microbicide compositions of the present invention has protection concurrently, treats and root out activity, can be used for various crop Fructus Vitis viniferae, Fructus Cucumidis sativi,
Fructus Capsici, Fructus Lycopersici esculenti, Nicotiana tabacum L., Semen Tritici aestivi, Oryza sativa L., Semen arachidis hypogaeae, vegetable, fruit tree, flowers, lawn etc., preventing and treating capsule bacterium subphylum, Basidiomycotina and
The microbial plant disease of cause of disease of Deuteromycotina, especially to the downy mildew on preventing and treating vegetable, fruit tree, Nicotiana tabacum L., early blight, evening
Epidemic disease, balck shank effect are especially pronounced.
The bactericidal composition of the present invention has the advantage that first, and said composition is Synergistic in certain ratio range
Effect performance is notable, not only can improve protection effect, reduces dosage, can expand again fungicidal spectrum simultaneously；Second, delaying drug resistance
Occur；3rd, chemical pesticide and biological pesticide compound, and improve safety, environmentally friendly.Therefore, this bactericidal composition has
Preferably economic benefit and environmental benefit, have bigger application value.
Detailed description of the invention
Below in conjunction with embodiment to further instruction of the present invention, the percentage ratio in embodiment is mass percent, but this
Bright it is not limited thereto.
Implementation of an application example one: the Toxicity Determination test that amisulbrom and amino-oligosaccharide compound
The Toxicity Determination of downy mildew of garpe is tested by toxicity test embodiment 1
Subjects: Plasmopara viticola (Plasmopara uiticola(Berk.dt Curtis)Berl. Et de Toni)
Test method: survey standard method NY/T 1156.3-2006 with reference to raw, use plate blade method.
Select incidence of leaf, with 4 DEG C of distillation washing lower blade back side Pseudoperonospora cubensis Sporangiums, be made into suspension (concentration control
System is at every milliliter 1 × 105Individual ~ 1 × 107Individual Sporangium), deposit standby at 4 DEG C.The variable concentrations medicinal liquid prepared uniformly is sprayed
Impose on vacuum side of blade, after medicinal liquid natural air drying, each process blade and blade is gone up dorsad, be emitted on moisture preservation box according to after processing labelling
In.After chemicals treatment 24 hours, it is inoculated in vacuum side of blade with ready Fresh spores capsule suspension drop 10uL.Every blade
Inoculating 4, often process 5 leaves, test sets the process without medicament and makees blank.Ware lid on inoculation bonnet, is placed in artificial gas
Waiting case, in continuous illumination every day/dark 12 hours alternately, temperature is 17 DEG C ~ 22 DEG C, trains under conditions of relative humidity more than 90%
Support.
Measuring and record lesion diameter depending on process and blank incidence, unit is millimeter, calculates prevention effect.Logical
Cross the linear regression analysis between the probit value of prevention effect and the logarithm value of series concentration, obtain the EC of each medicament50Value.Use grandson
Yun Peifa calculates the co-toxicity coefficient (CTC) of mixture, evaluates the reagent agent activity to pathogenic bacteria with this.
Prevention effect (P) %=[blank lesion diameter (D0)-process lesion diameter (D1)]/blank scab is straight
Footpath (D0)×100
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) × 100
Percent (%)+medicament B that mixture theory toxicity index (TTI)=medicament A toxicity index × A medicament is shared in mixture
The percent (%) shared in mixture of toxicity index × B medicament
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100
Co-toxicity coefficient CTC >=120 of complex preparation show as potentiation, and 80 ＜ CTC ＜ 120 show as summation action, and CTC≤
80 show as antagonism.Toxicity Determination result of the test, refers to table 1.
As shown in Table 1, amisulbrom and amino-oligosaccharide are when proportioning is between 100:1 ~ 1:100, and co-toxicity coefficient is all
More than 120, downy mildew of garpe being had potentiation, time especially between 20:1 ~ 1:20, co-toxicity coefficient is more than 180.
The Toxicity Determination of tomato late blight is tested by toxicity test embodiment 2
Subjects: Phytophthora infestans pathogenic bacteria (Phytophthora infestans(Mont.)de Bary)
Test method: survey standard method NY/T 1156.2-2006 with reference to raw, use pastille medium therapy: take each single dose and mixture
The medicinal liquid 3mL of series concentration, adds in the PDA culture medium of the 27mL being cooled to 45 DEG C, makes the pastille training of required final concentration
Support base flat board.Then, produce 6mm diameter mycelia block from the Phytophthora infestans pathogenic bacteria colony edge cultivated 7 days, move to each series pastille
In culture medium, mycelia faces down, and each process is repeated 4 times.It is disposed, is placed in the constant temperature biochemical cultivation case of 25 DEG C cultivation, 4
Measure colony diameter after it, calculate growth inhibition ratio.By the line between probit value and the logarithm value of series concentration of suppression ratio
Property regression analysis, obtains the EC of each medicament50Value, calculates the co-toxicity coefficient (CTC) of mixture, evaluates confession with this by the abundant method of Sun Yun
The reagent agent activity to pathogenic bacteria.
[blank bacterium colony increases diameter (D to growth inhibition ratio (I) %=0)-chemicals treatment bacterium colony increases diameter (D1)]/empty
White comparison bacterium colony increases diameter (D0)×100
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) × 100
Percent (%)+medicament B that mixture theory toxicity index (TTI)=medicament A toxicity index × A medicament is shared in mixture
The percent (%) shared in mixture of toxicity index × B medicament
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100
Co-toxicity coefficient CTC >=120 of complex preparation show as potentiation, and 80 ＜ CTC ＜ 120 show as summation action, and CTC≤
80 show as antagonism.Toxicity Determination result of the test, refers to table 2.
As shown in Table 2, amisulbrom and amino-oligosaccharide are when proportioning is between 100:1 ~ 1:100, and co-toxicity coefficient is all
More than 120, tomato late blight being had potentiation, time especially between 20:1 ~ 1:20, co-toxicity coefficient is more than 180.
The Toxicity Determination of black shank is tested by toxicity test embodiment 3
Subjects: black shank( Phytophtora parasitica var.nicotianae Tucker)
Test method: survey standard method NY/T 1156.2-2006 with reference to raw, use pastille medium therapy: take each single dose and mixture
The medicinal liquid 3mL of series concentration, adds in the PDA culture medium of the 27mL being cooled to 45 DEG C, makes the pastille training of required final concentration
Support base flat board.Then, produce 6mm diameter mycelia block from the tobacco black shank bacterium colony edge cultivated 7 days, move to each series pastille
In culture medium, mycelia faces down, and each process is repeated 4 times.It is disposed, is placed in the constant temperature biochemical cultivation case of 25 DEG C cultivation, 4
Measure colony diameter after it, calculate growth inhibition ratio.By the line between probit value and the logarithm value of series concentration of suppression ratio
Property regression analysis, obtains the EC of each medicament50Value, calculates the co-toxicity coefficient (CTC) of mixture, evaluates confession with this by the abundant method of Sun Yun
The reagent agent activity to pathogenic bacteria.
[blank bacterium colony increases diameter (D to growth inhibition ratio (I) %=0)-chemicals treatment bacterium colony increases diameter (D1)]/empty
White comparison bacterium colony increases diameter (D0)×100
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) × 100
Percent (%)+medicament B that mixture theory toxicity index (TTI)=medicament A toxicity index × A medicament is shared in mixture
The percent (%) shared in mixture of toxicity index × B medicament
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100
Co-toxicity coefficient CTC >=120 of complex preparation show as potentiation, and 80 ＜ CTC ＜ 120 show as summation action, and CTC≤
80 show as antagonism.Toxicity Determination result of the test, refers to table 3.
As shown in Table 3, amisulbrom and amino-oligosaccharide are when proportioning is between 100:1 ~ 1:100, and co-toxicity coefficient is all
More than 120, black shank being had potentiation, time especially between 20:1 ~ 1:20, co-toxicity coefficient is more than 180.
Implementation of an application example two: (following content is folding for amisulbrom and amino-oligosaccharide compound product example of formulations
Hundred percentage compositions), but the present invention is not limited to the proportion in following example.
Example of formulations 1 25.5% amisulbrom amino-oligosaccharide suspending agent
Amisulbrom 25%, amino-oligosaccharide 0.5%, naphthalene sulfonic acid-formaldehyde condensation product sodium salt (dispersant) 6%, wetting and penetrating agent F
(wetting agent) 4%, silicone oil (defoamer) 0.5%, aluminium-magnesium silicate (thickening agent) 0.1%, propylene glycol (antifreezing agent) 5%, deionized water add
To 100%, prepare 25.5% amisulbrom amino-oligosaccharide suspending agent.
Example of formulations 2 42% amisulbrom amino-oligosaccharide suspending agent
Amisulbrom 40%, amino-oligosaccharide 2%, aliphatic amine polyoxyethylene ether (dispersant) 6%, Bombyx mori L. (wetting agent) 6%, C8~10
Fatty alcohol (defoamer) 0.4%, hydroxymethyl cellulose (thickening agent) 0.3%, ethylene glycol (antifreezing agent) 6%, deionized water add to
100%, prepare 42% amisulbrom amino-oligosaccharide suspending agent.
Example of formulations 3 11% amisulbrom amino-oligosaccharide suspending agent
Amisulbrom 1%, amino-oligosaccharide 10%, alkyl benzene sulfonate calcium salt (dispersant) 4%, Fructus Sapindi Mukouossi powder (wetting agent) 6%, have
Machine silicone (defoamer) 0.5%, xanthan gum (thickening agent) 0.4%, glycerol (antifreezing agent) 7%, deionized water add to 100%, prepare
11% amisulbrom amino-oligosaccharide suspending agent.
Example of formulations 4 10.2% amisulbrom amino-oligosaccharide suspension emulsion
Amisulbrom 0.2%, amino-oligosaccharide 10%, castor oil polyoxyethylene ether (emulsifying agent) 8%, alkylphenol polyoxyethylene
(dispersant) 5%, capric acid (defoamer) 0.5%, methylcellulose (thickening agent) 0.1%, ethylene glycol (antifreezing agent) 0.1%, fumaric acid
Dimethyl ester (preservative) 0.1%, deionized water add to 100%, prepare 10.2% amisulbrom amino-oligosaccharide suspension emulsion.
Example of formulations 5 21% amisulbrom amino-oligosaccharide suspension emulsion
Amisulbrom 1%, amino-oligosaccharide 20%, sorbester p17 (emulsifying agent) the 6%, (dispersion of octylphenol polyethylene oxyethylene group ether sulfate
Agent) 6%, silicone oil (defoamer) 0.6%, hydroxyethyl cellulose (thickening agent) 0.2%, Polyethylene Glycol (antifreezing agent) 0.2%, sodium propionate
(preservative) 0.2%, deionized water add to 100%, prepare 21% amisulbrom amino-oligosaccharide suspension emulsion.
Example of formulations 6 44% amisulbrom amino-oligosaccharide suspension emulsion
Amisulbrom 40%, amino-oligosaccharide 4%, OP-10 phosphate ester (emulsifying agent) 8%, alkylnaphthalene sulfonate (dispersant) 8%,
Organic silicone (defoamer) 0.2%, polyvinyl alcohol (thickening agent) 0.3%, glycerol (antifreezing agent) 0.3%, sodium benzoate (preservative)
0.1%, deionized water adds to 100%, prepares 44% amisulbrom amino-oligosaccharide suspension emulsion.
Example of formulations 7 20.2% amisulbrom amino-oligosaccharide microemulsion
Amisulbrom 0.2%, amino-oligosaccharide 20%, DMF (solvent) 15%, alkyl benzene sulfonate calcium salt (breast
Agent) 10%, Polyethylene Glycol (antifreezing agent) 0.3%, hydroxymethyl cellulose (thickening agent) 0.3%, organic silicone (defoamer) 0.2%,
Sodium diacetate (preservative) 0.1%, deionized water add to 100%, prepare 20.2% amisulbrom amino-oligosaccharide microemulsion.
Example of formulations 8 55% amisulbrom amino-oligosaccharide microemulsion
Amisulbrom 5%, amino-oligosaccharide 50%, NOP (solvent) 10%, triethanolamine salt (emulsifying agent) 6%,
Propylene glycol (antifreezing agent) 0.5%, xanthan gum (thickening agent) 0.4%, silicone oil (defoamer) 0.1%, potassium sorbate (preservative) 0.05%,
Deionized water adds to 100%, prepares 55% amisulbrom amino-oligosaccharide microemulsion.
Example of formulations 9 51% amisulbrom amino-oligosaccharide microemulsion
Amisulbrom 50%, amino-oligosaccharide 1%, 3-methyl cyclohexanol ketenes (solvent) 5%, styrene polyoxyethylene ether ammonium sulfate
Salt (emulsifying agent) 6%, ethylene glycol (antifreezing agent) 0.3%, hydroxyethyl cellulose (thickening agent) 0.3%, C8~10Fatty alcohol (defoamer)
0.2%, butyl p-hydroxybenzoate (preservative) 0.05%, deionized water add to 100%, prepare 51% amisulbrom amino few
Sugar element microemulsion.
Example of formulations 10 5.1% amisulbrom amino-oligosaccharide aqueous emulsion
Amisulbrom 0.1%, amino-oligosaccharide 5%, N-ketopyrrolidine (solvent) 8%, 506#(emulsifying agent) 6%, Polyethylene Glycol (anti-
Freeze agent) 0.2%, hydroxymethyl cellulose (thickening agent) 0.3%, organic silicone (defoamer) 0.5%, deionized water add to 100%, prepare
5.1% amisulbrom amino-oligosaccharide aqueous emulsion.
Example of formulations 11 20.2% amisulbrom amino-oligosaccharide aqueous emulsion
Amisulbrom 0.2%, amino-oligosaccharide 20%, ethylene glycol monomethyl ether (solvent) 6%, NP-10 phosphate ester (emulsifying agent) 6%, second
Glycol (antifreezing agent) 0.3%, aluminium-magnesium silicate (thickening agent) 0.3%, C8~10Fatty alcohol (defoamer) 0.2%, deionized water add to
100%, prepare 20.2% amisulbrom amino-oligosaccharide aqueous emulsion.
Example of formulations 12 11% amisulbrom amino-oligosaccharide aqueous emulsion
Amisulbrom 10%, amino-oligosaccharide 1%, ethyl acetate (solvent) 8%, 600#(emulsifying agent) 6%, propylene glycol (antifreezing agent)
0.3%, xanthan gum (thickening agent) 0.3%, silicone oil (defoamer) 0.2%, deionized water add to 100%, prepare 11% amisulbrom
Amino-oligosaccharide aqueous emulsion.
Example of formulations 13 10.5% amisulbrom amino-oligosaccharide microcapsule suspending agent
Amisulbrom 10%, amino-oligosaccharide 0.5%, ethyl acetate (solvent) 6%, triethanolamine salt (emulsifying agent) 6%, toluene two
Isocyanates (encystation monomer) 2%, polycarboxylate (dispersant) 6%, Polyethylene Glycol (antifreezing agent) 0.3%, methylcellulose (thickening
Agent) 0.3%, butyl p-hydroxybenzoate (preservative) 0.05%, deionized water add to 100%, prepare 10.5% amisulbrom ammonia
Base oligosaccharide microcapsule suspending agent.
Example of formulations 14 20% amisulbrom amino-oligosaccharide microcapsule suspending agent
Amisulbrom 10%, amino-oligosaccharide 10%, 3-methyl cyclohexanol ketenes (solvent) 8%, alkyl benzene sulfonate calcium salt (emulsifying agent)
6%, methyl diphenylene diisocyanate (encystation monomer) 1%, lignosulfonates (dispersant) 6%, glycerol (antifreezing agent)
0.2%, aluminium-magnesium silicate (thickening agent) 0.4%, sodium diacetate (preservative) 0.1%, deionized water add to 100%, prepare 20% indazole sulphur
Bacterium amine amino-oligosaccharide microcapsule suspending agent.
Example of formulations 15 10.5% amisulbrom amino-oligosaccharide microcapsule suspending agent
Amisulbrom 0.5%, amino-oligosaccharide 10%, ethyl acetate (solvent) 6%, phenethyl phenol polyethenoxy ether (emulsifying agent)
6%, polyether polyol (encystation monomer) 1%, naphthalene sulfonic acid-formaldehyde condensation product (dispersant) 5%, Polyethylene Glycol (antifreezing agent) 0.2%, Huang
Virgin rubber (thickening agent) 0.4%, potassium sorbate (preservative) 0.1%, deionized water add to 100%, prepare 10.5% amisulbrom ammonia
Base oligosaccharide microcapsule suspending agent.
Example of formulations 16 22% amisulbrom amino-oligosaccharide microcapsule suspension-suspendinagent agent
Amisulbrom 20%, amino-oligosaccharide 2%, ethyl acetate (solvent) 8%, phenethyl phenol polyethenoxy ether (emulsifying agent) 6%,
Toluene di-isocyanate(TDI) (encystation monomer) 3%, sodium lauryl sulphate (wetting agent) 6%, Polyethylene Glycol (antifreezing agent) 0.3%, hydroxyl first
Base cellulose (thickening agent) 0.3%, C8~10Fatty alcohol (defoamer) 0.2%, deionized water add to 100%, prepare 22% indazole sulphur bacterium
Amine amino-oligosaccharide microcapsule suspension-suspendinagent agent.
Example of formulations 17 11% amisulbrom amino-oligosaccharide microcapsule suspension-suspendinagent agent
Amisulbrom 1%, amino-oligosaccharide 10%, ethylene glycol monomethyl ether (solvent) 6%, alkyl benzene sulfonate calcium salt (emulsifying agent) 6%, poly-
Ethoxylated polyhydric alcohol (encystation monomer) 1%, pull open powder X(wetting agent) 6%, glycerol (antifreezing agent) 0.3%, xanthan gum (thickening agent) 0.5%,
Silicone oil (defoamer) 0.2%, deionized water add to 100%, prepare 11% amisulbrom amino-oligosaccharide microcapsule suspension-suspension
Agent.
Example of formulations 18 21% amisulbrom amino-oligosaccharide microcapsule suspension-suspendinagent agent
Amisulbrom 1%, amino-oligosaccharide 20%, Ketohexamethylene (solvent) 8%, 700#(emulsifying agent) 8%, diphenyl methane two isocyanide
Acid esters (encystation monomer) 3%, spaonin powder (wetting agent) 5%, ethylene glycol (antifreezing agent) 0.3%, polyvinyl alcohol (thickening agent) 0.5%, have
Machine silicone (defoamer) 0.2%, deionized water add to 100%, prepare 21% amisulbrom amino-oligosaccharide microcapsule suspension-suspension
Agent.
Example of formulations 19 50.5% amisulbrom amino-oligosaccharide wettable powder
Amisulbrom 50%, amino-oligosaccharide 0.5%, alkylphenol polyoxyethylene formaldehyde condensation products sulfate (dispersant) 8%,
Spaonin powder (wetting agent) 6%, bentonite add to 100%, prepare 50.5% amisulbrom amino-oligosaccharide wettable powder.
Example of formulations 20 84% amisulbrom amino-oligosaccharide wettable powder
Amisulbrom 80%, amino-oligosaccharide 4%, alkylnaphthalene sulfonate (dispersant) 6%, sodium lauryl sulphate (wetting agent)
6%, kieselguhr adds to 100%, prepares 84% amisulbrom amino-oligosaccharide wettable powder.
Example of formulations 21 60% amisulbrom amino-oligosaccharide wettable powder
Amisulbrom 30%, amino-oligosaccharide 30%, lignosulfonates (dispersant) 8%, Fructus Sapindi Mukouossi powder (wetting agent) 6%, height
Ridge soil adds to 100%, prepares 60% amisulbrom amino-oligosaccharide wettable powder.
Example of formulations 22 51% amisulbrom amino-oligosaccharide water dispersible granules
Amisulbrom 50%, amino-oligosaccharide 1%, polyoxyethylene carboxylate (dispersant) 6%, pull open powder (wetting agent) 8%, sulfur
Acid ammonium (disintegrating agent) 5%, precipitated calcium carbonate add to 100%, prepare 51% amisulbrom amino-oligosaccharide water dispersible granules.
Example of formulations 23 80% amisulbrom amino-oligosaccharide water dispersible granules
Amisulbrom 40%, amino-oligosaccharide 40%, naphthalene sulfonic acid-formaldehyde condensation product (dispersant) 6%, Bombyx mori L. (wetting agent) 6%, urine
Element (disintegrating agent) 4%, attapulgite add to 100%, prepare 80% amisulbrom amino-oligosaccharide water dispersible granules.
Example of formulations 24 25.5% amisulbrom amino-oligosaccharide water dispersible granules
Amisulbrom 0.5%, amino-oligosaccharide 25%, polycarboxylate (dispersant) 6%, dodecylbenzene sodium sulfonate (wetting agent)
8%, citric acid (disintegrating agent) 5%, white carbon add to 100%, prepare 25.5% amisulbrom amino-oligosaccharide water dispersible granules.
Implementation of an application example three: the field control effectiveness test that amisulbrom is compounding with amino-oligosaccharide
Field information embodiment 1 prevents and treats the field control effectiveness test of downy mildew of garpe
Test site: this test arrangement, in Bishan County, Chongqing City, is chosen downy mildew and fallen ill heavier vineyard throughout the year.
Test method: with reference to " pesticide field efficacy medicine test criterion (two) bactericidal agent for preventing and treating downy mildew of garpe GB/T
17980.122-2004 ", test medicine, the community of blank process and use random alignment, each community 8 ~ 15 strain, often process
Repeat for 4 times, process as blank using equivalent clear water.Test medicine and consumption are shown in Table 4, and application process is spraying, until leaf
The positive and negative of sheet uniformly squirts.Dispenser is started at downy mildew of garpe their early stage, whole period of duration dispenser 3 times, dispenser interval 7
~ 10 days.
Investigation and statistical method: every community random searching 10 pumping then is the most climing, investigates whole blade from top to bottom, presses
Following stage division records the sick number of sheets at different levels and total number of sheets.Generally investigate state of an illness radix before dispenser, before dispenser next time and last is executed
7 days ~ 14 days investigation prevention effects after medicine, record spraying times, each dispenser date and Crop growing stage.
Blade stage division:
0 grade: without scab；
1 grade: lesion area accounts for less than the 5% of whole leaf area；
3 grades: lesion area accounts for the 6% ~ 25% of whole leaf area；
5 grades: lesion area accounts for the 26% ~ 50% of whole leaf area；
7 grades: lesion area accounts for the 51% ~ 75% of whole leaf area；
9 grades: lesion area accounts for more than the 76% of whole leaf area.
Drug effect metering method:
Disease index=Σ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Sick leaf rate (%)=sick number of sheets/investigate the total number of sheets × 100
Prevention effect (%)=[before 1-(blank district medicine after disease index × treatment region medicine after disease index/blank district medicine
Disease index before disease index × treatment region medicine)] × 100
As can be known from Table 4, embodiment of the present invention bactericidal composition amisulbrom and amino-oligosaccharide are preventing and treating downy mildew of garpe
On be all significantly increased, after last medicine 7 days, the highest preventive effect reached 96.30%, and minimum preventive effect is 94.55%；And indazole sulphonamide,
The preventive effect of amino-oligosaccharide single dose is respectively 81.08% and 79.06%.Field test results fully shows, amisulbrom and ammonia
Base oligosaccharide is compounding has obvious synergistic function to downy mildew of garpe, in the range of this test dosage, pacifies Fructus Vitis viniferae
Entirely, have no adverse effects, and situation about reducing in effective ingredient dosage, preventive effect increases substantially.As can be seen here, present invention combination
Thing has reduction dosage, improves preventive effect, reduction cost, minimizing residual, the advantage of safety and environmental protection.
Field information embodiment 2 prevents and treats the field control effectiveness test of tomato late blight
Test site: this test arrangement, at Chongqing City's Yunyang County, is chosen tomato late blight and fallen ill heavier plot throughout the year.
Test method: with reference to " pesticide field efficacy medicine test criterion (one) bactericidal agent for preventing and treating tomato late blight GB/
T17980.31-2000 ", test medicine, the community of blank process and use random alignment, plot area 30m2, often process 4
Secondary repetition, processes as blank using equivalent clear water.Test medicine and consumption are shown in Table 5, and application process is spraying, until blade
Positive and negative uniformly squirt.Dispenser is started at tomato late blight their early stage, whole period of duration dispenser 2 times, dispenser interval 7 ~
10 days.
Investigation and statistical method: every random 5 samplings in community, often click two strains, and every strain divides upper, middle and lower to investigate 10
Leaf, the percentage rate accounting for whole leaf area with the lesion area on every a piece of leaf carrys out classification.State of an illness radix is investigated before dispenser, last
Secondary investigation is carried out for 7 ~ 10 days after last dispenser.
Stage division:
0 grade: without scab；
1 grade: lesion area accounts for less than the 5% of whole leaf area；
3 grades: lesion area accounts for the 6% ~ 10% of whole leaf area；
5 grades: lesion area accounts for the 11% ~ 20% of whole leaf area；
7 grades: lesion area accounts for the 21% ~ 50% of whole leaf area；
9 grades: lesion area accounts for more than the 50% of whole leaf area.
Drug effect metering method:
Disease index=Σ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Prevention effect (%)=[before 1-(blank district medicine after disease index × treatment region medicine after disease index/blank district medicine
Disease index before disease index × treatment region medicine)] × 100
As can be known from Table 5, embodiment of the present invention bactericidal composition amisulbrom and amino-oligosaccharide are at preventing and treating tomato late blight
On be all significantly increased, after last medicine 10 days, the highest preventive effect reached 95.75%, and minimum preventive effect is 94.40%；And indazole sulphonamide,
The preventive effect of amino-oligosaccharide single dose is respectively 75.29% and 73.02%.Field test results fully shows, amisulbrom and ammonia
Base oligosaccharide is compounding has obvious synergistic function to tomato late blight, in the range of this test dosage, pacifies Fructus Lycopersici esculenti
Entirely, have no adverse effects, and situation about reducing in effective ingredient dosage, preventive effect increases substantially.As can be seen here, present invention combination
Thing has reduction dosage, improves preventive effect, reduction cost, minimizing residual, the advantage of safety and environmental protection.
Field information embodiment 3 prevents and treats the field control effectiveness test of black shank
Test site: this test arrangement, in Peng River county of Chongqing City, is chosen black shank and fallen ill heavier plot throughout the year.
Test method: with reference to " pesticide field efficacy medicine test criterion (two) bactericidal agent for preventing and treating black shank GB/
T17980.90-2004 ", test medicine, the community of blank process and use random alignment, plot area 40m2, often process 4
Secondary repetition, processes as blank using equivalent clear water.Test medicine and consumption are shown in Table 7, and application process is pouring root, black Nicotiana tabacum L.
Shin disease their early stage starts dispenser, whole period of duration dispenser 3 times, 7 ~ 10 days intervals of dispenser.
Investigation and statistical method: every community uses five point sampling investigation, and every takes 5 strain ~ 10 strains, and total strain number investigated in record
And diseased plant number at different levels.Before dispenser investigate state of an illness radix, next time dispenser before and last dispenser after 7d ~ 14d investigation prevention effect.
Stage division:
0 grade: Herb is anosis；
1 grade: stem's scab is less than 1/3rd of stem girth, and indivedual blades are wilted；
3 grades: stem's scab is less than 1/2nd of stem girth, or half is the most wilting with lower blade, or bottom minority blade goes out
Existing scab；
5 grades: stem's scab exceedes 1/2nd of stem girth, or blade more than half is the most wilting；
7 grades: stem's scab is around stem girth, or 2/3rds is wilting with blade；
9 grades: the whole blade of diseased plant is wilting or withered.
Drug effect metering method:
Disease index=Σ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Prevention effect (%)=[before 1-(blank district medicine after disease index × treatment region medicine after disease index/blank district medicine
Disease index before disease index × treatment region medicine)] × 100
As can be known from Table 6, embodiment of the present invention bactericidal composition amisulbrom and amino-oligosaccharide are at preventing and treating black shank
On be all significantly increased, after last medicine 14 days, the highest preventive effect reached 95.43%, and minimum preventive effect is 93.65%；And indazole sulphonamide,
The preventive effect of amino-oligosaccharide single dose is respectively 72.72% and 72.31%.Field test results fully shows, amisulbrom and ammonia
Base oligosaccharide is compounding has obvious synergistic function to black shank, in the range of this test dosage, pacifies Nicotiana tabacum L.
Entirely, have no adverse effects, and situation about reducing in effective ingredient dosage, preventive effect increases substantially.As can be seen here, present invention combination
Thing has reduction dosage, improves preventive effect, reduction cost, minimizing residual, the advantage of safety and environmental protection.
In sum, bactericidal composition amisulbrom of the present invention and amino-oligosaccharide compound, not only to Fructus Vitis viniferae
Downy mildew, tomato late blight, black shank have a significant potentiation, simultaneously to cucumber downy mildew, celery cabbage clubroot, peppery
Green pepper epidemic disease, Nicotiana tabacum L. epidemic disease, Citrullus vulgaris epidemic disease etc. also have preferable prevention effect.Its activity and bactericidal effect are not each composition activities
Simple superposition, compared with existing unitary agent, in addition to having significant bactericidal effect, and has significant potentiation, right
Crop safety is good, meets the security requirement of pesticidal preparations.
Claims (6)
1. one kind contains amisulbrom and the bactericidal composition of amino-oligosaccharide and application thereof, it is characterised in that: described sterilization
Compositions contains effective ingredient amisulbrom and effective ingredient amino-oligosaccharide, and both mass ratioes are 100:1 ~ 1:100.
Bactericidal composition the most according to claim 1, it is characterised in that: effective ingredient amisulbrom and effective ingredient ammonia
Base oligosaccharide, both mass ratioes are preferably 50:1 ~ 1:50.
Bactericidal composition the most according to claim 2, it is characterised in that: effective ingredient amisulbrom and effective ingredient ammonia
Base oligosaccharide, both mass ratioes are preferably 20:1 ~ 1:20.
Bactericidal composition the most according to any one of claim 1 to 3, it is characterised in that: bactericidal composition effectively becomes
The gross mass percentage composition divided is 5% ~ 85%.
Bactericidal composition the most according to claim 4, it is characterised in that: compositions can be made into suspending agent, suspension emulsion, microemulsion
Agent, aqueous emulsion, microcapsule suspending agent, microcapsule suspension-suspendinagent agent, wettable powder, water dispersible granules.
Bactericidal composition the most according to claim 5 has the downy mildew on preventing and treating vegetable, fruit tree, Nicotiana tabacum L., early blight, evening
The purposes of the disease such as epidemic disease, balck shank.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719662A (en) * | 2017-01-13 | 2017-05-31 | 佛山市盈辉作物科学有限公司 | Bactericidal composition comprising Hexanaphthene flusulfamide and amino-oligosaccharide |
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| WO2003053145A1 (en) * | 2001-12-21 | 2003-07-03 | Nissan Chemical Industries, Ltd. | Bactericidal composition |
| CN1949970A (en) * | 2004-04-30 | 2007-04-18 | 巴斯福股份公司 | Fungicidal mixtures |
| CN101052302A (en) * | 2004-10-22 | 2007-10-10 | 石原产业株式会社 | Agricultural or horticultural bactericidal composition and method of controlling plant disease |
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003053145A1 (en) * | 2001-12-21 | 2003-07-03 | Nissan Chemical Industries, Ltd. | Bactericidal composition |
| CN1949970A (en) * | 2004-04-30 | 2007-04-18 | 巴斯福股份公司 | Fungicidal mixtures |
| CN101052302A (en) * | 2004-10-22 | 2007-10-10 | 石原产业株式会社 | Agricultural or horticultural bactericidal composition and method of controlling plant disease |
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106719662A (en) * | 2017-01-13 | 2017-05-31 | 佛山市盈辉作物科学有限公司 | Bactericidal composition comprising Hexanaphthene flusulfamide and amino-oligosaccharide |
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