CN102349524B - Sterilizing composition containing Cyflufenamid - Google Patents
Sterilizing composition containing Cyflufenamid Download PDFInfo
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Abstract
The invention relates to a sterilizing composition containing Cyflufenamid, which is obtained by the binary compounding of the effective ingredients, namely Cyflufenamid and dithiocarbamate compounds, wherein the weight percentage ratio of the two effective ingredients is (90-1): (1-90), preferably (50-3): (10-90); and the total percentage by weight of the total effective ingredient in the sterilizing composition is 1-90%, preferably 10-80%, and the balance is the auxiliary element which is permitted to be used and can be accepted in the pesticide. The known method can be used for preparing the sterilizing composition into wettable powder, suspending agent, powder, microemulsion, emulsion in water and water dispersible granules. Cyflufenamid and dithiocarbamate compounds serve as effective ingredients to prevent and control bacterial diseases and fungoid diseases on various plants, such as vegetables, fruit trees, grains, ornamental plants and the like.
Description
Technical field
The present invention relates to the agriculture chemical compounding technical field, be specifically related to a kind of one of any as two yuan of composite bactericidal compositions of active ingredient in cyflufenamid and thiocarbamate compounds zineb, Propineb, Carbatene, mancozeb, the ziram.
Background technology
Cyflufenamid is the bactericide with novel precursor structure of Japanese Cao Da company exploitation, and its chemical structural formula is as follows:
It has good effect and characteristic to powdery mildew, the peach grey scab bacterium of various vegetables and wheat class, simultaneously benzimidazole, imidazoles, methoxy acrylate class resistance bacterium and sensitive bacteria is had same isoreactivity.Cyflufenamid is extremely low to people and animals' toxicity, do not exist follow-on impact, teratogenesis, mutagenicity test all is negative, on producing also not to stimulation and the allergy of eye, skin.In addition, this agent can not affect environment and beneficial organism yet, and is minimum on honeybee and silkworm impact yet.
Zineb is the protectiveness organophosphorus fungicide, the formal name used at school zinc ethylene bisdithiocarbamate, be mainly used in the blade face protection, can prevent and treat multiple fungus-caused disease, be broad-spectrum germicide, can prevent and treat the multiple diseases of paddy rice, fruit tree, vegetables and tobacco, such as tomato blight, anthracnose, ring spot; Brassicaceous vegetable and beans soft rot, anthracnose, soybean rust; The scab of pears, apple; Anthracnose of tobacco, rust; Wheat rust; Rice sheath blight disease etc.
Propineb is a kind of wide spectrum, quick-acting protective fungicide; be applicable to Chinese cabbage downy mildew, cucumber downy mildew, the early sick control such as late blight, mango anthracnose of tomato of tomato, Chinese cabbage, cucumber, mango and flowers and other crops; the downy mildew of the crops such as vegetables, tobacco, lupulus and morning, the late blight of tomato and potato all there are good protective effect, and the germ disease of powdery mildew, rust and Botrytis is also had certain inhibitory action.
Carbatene (metiram) chemical name: the co-ordination complex of the two two sulphur deoxidation base formic acid manganese of ethylene and zinc ion, molecular formula: (C
4H
6N
2S
4Zn) X is protective fungicide, and the fungus-caused various diseases of Oomycete are had good preventive effect.
Mancozeb (mancozeb) molecular formula: C
4H
6N
2S
4The MnZn chemical name: the complex salt of the two two thiocarbamoyl manganese of ethylene and zinc is the complex that maneb and zinc form, and belongs to the wider protective fungicide of fungicidal spectrum.It mainly is the oxidation that suppresses pyruvic acid in the thalline.Effective to multiple diseases such as the anthracnose on fruit tree, the vegetables, early blight, simultaneously it often is mixed with systemic fungicide, for the generation of delaying drug resistance.
Ziram, a kind of wide spectrum, protectiveness good fortune U.S.A is organosulfureous fungicide.Alternariaspp, Septoria musiva, botrytis, black star germ, downy mildew, tail spore bacterium etc. there is activity.Normal operation concentration is to crop safety, but the responsive easily crude drug of tobacco and some Curcurbitaceae vegetables evil.The Common Diseases of sprayable control fruit tree, vegetables, such as apple flower maize ear rot, powdery mildew, pear scab, peach shot hole, brown rot, bitter rot or anthracnose of grape and various vegetables spot blight, downy mildew, gray mold, early blight etc.
Above bactericide is long-term to be used separately, and preventive effect begins to descend, and has produced pesticide resistance to a certain degree, simultaneously, causes dosage to strengthen, and the problems such as lasting period shortening are unfavorable for the sustainable development of environment very much.The inventor attempts with the new type bactericide of the different mechanisms of action composite with it, to improve its preventive effect, reduce its agricultural cost, through the test of indoor virulence repeatedly and field experiment, in the formula indoor screening, chance on, cyflufenamid is mixed with the arbitrary fungicide active ingredient that is selected from thiocarbamate class (zineb, Propineb, Carbatene, mancozeb, ziram) the target bacterium is all had extremely strong synergistic effect, has greatly improved bactericidal activity, thereby has finished the present invention.And about cyflufenamid and be selected from arbitrary fungicide active ingredient composite of thiocarbamate class (zineb, Propineb, Carbatene, mancozeb, ziram), there is not yet at present relevant report at home and abroad.
Summary of the invention
I research staff of company is devoted for years in the research in the composite field of agriculture chemicals fungicide, through a large amount of formulation screening tests, a kind of bactericidal composition of new high-efficiency has been proposed, bactericidal composition of the present invention is with new type bactericide cyflufenamid and thiocarbamate compounds zineb, Propineb, Carbatene, mancozeb, one of any two yuan of carrying out for active ingredient are mixed in the ziram, be used for preventing and treating the powdery mildew of various crop, rust, downy mildew, epidemic disease, leaf spot, anthracnose, black spot of fruit tree, spot defoliation, the disease such as shot hole and rice blast, can obviously improve actual preventive effect, delay the drug-fast generation of germ, reduce drug cost.
The purpose of this invention is to provide the low bactericidal composition of a kind of efficient, low toxicity, use cost to satisfy the needs of agricultural production.Produce synergistic effect after two kinds of active component combinations, when improving preventive effect, can also avoid the drug-fast generation of germ and delay the drug-fast generation speed of germ.
The objective of the invention is to realize by following measures:
A kind of bactericidal composition based on cyflufenamid, it is characterized in that two yuan that in cyflufenamid (representing with A) and thiocarbamate compounds zineb, Propineb, Carbatene, mancozeb, the ziram one of any (representing with B) carry out as fungicide active ingredient composite, the percentage by weight of effective constituents A and B is 90～1: 1～90.
Wherein thiocarbamate compounds of the present invention is: zineb, Propineb, Carbatene, mancozeb, ziram.
Based on a large amount of specific aim experimental formula screenings, described with one or two yuan of any composite bactericidal composition in A cyflufenamid and B thiocarbamate compounds zineb, Propineb, Carbatene, mancozeb, the ziram, effectively the percentage by weight of fungicide active ingredient A and B can be more preferably 50～3: 10～90.
Described take (A) cyflufenamid and (B) a kind of thiocarbamate compounds as the bactericidal composition of effective fungicide active ingredient, can be prepared into wetting powder, suspending agent, pulvis, microemulsion, aqueous emulsion and water dispersible granules with known method, the quality percentage composition of active ingredient is 1%～90% in the preparation, be preferably 10%～80%, all the other are usual auxiliaries in the agricultural chemicals.
Wherein prepare required necessary basis component and simply prepare implementation method as follows among the present invention about the formulations of pesticide:
One of technical scheme of the present invention, described bactericidal composition are wetting powder, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition wetting powder is: press above-mentioned prescription with A and B and dispersant, wetting agent and filler mixing, uniform stirring in stirred tank, behind airslide disintegrating mill, mixing, namely can be made into the wetting powder of the present composition.
Two of technical scheme of the present invention, described bactericidal composition are suspending agent, and the percentage by weight of component is:
The concrete production stage of this suspending agent is for mixing other auxiliary agents first, mix through high speed shear, add A and B, abrading-ball is 2～3 hours in ball crusher, make a diameter all below 5mm, make the suspending agent preparation of the bactericidal composition of the A of containing of the present invention and B.
Three of technical scheme of the present invention, described bactericidal composition are aqueous emulsion, and the percentage by weight of component is:
The concrete production stage of this aqueous emulsion is: at first former medicine A and B, solvent and emulsifier, cosolvent are added together, make and be dissolved into uniform oil phase; With part water, antifreeze, other the insecticides adjuvant such as antimicrobial mixes into uniform water; When the reactor high speed stirs, oil phase is added water, slowly add water until reach the phase inversion point, open clipper and carry out high speed shear, and add remaining water, shear approximately half an hour, form the aqueous emulsion of oil-in-water type.Namely make the aqueous emulsion of the bactericidal composition that contains A and B.
Four of technical scheme of the present invention, described bactericidal composition are water dispersible granules, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition water dispersible granules is: by above-mentioned prescription A, B and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediating, then add and carry out granulation, drying, screening in the fluidized bed prilling dryer by sample analysis, make the water dispersible granules of the bactericidal composition of the A of containing of the present invention and B.
Described emulsifier is selected from any one or more mixtures that form with arbitrary proportion in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, alkylphenol polyoxyethylene sulfosuccinate, styrylphenol polyoxyethylene ether, polyoxyethylene nonylphenol ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, the polyoxyethylene aliphatic alcohol ether.
Described solvent is any one or more mixed solvents that form with arbitrary proportion in dimethylbenzene or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), the water equal solvent.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, the fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, Nekal BX., among the wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, soapberry powder one or more.
Described disintegrant is selected from: one or more in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, the sodium bicarbonate.
Described thickener is selected from: one or more in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol.
Described stabilizing agent is selected from: a kind of in sodium citrate, the resorcinol.
Described antifreezing agent is selected from: one or more in ethylene glycol, propane diols, the glycerine.
Described defoamer is selected from: silicone oil, silicone compound, C
10-20Saturated fat acid compounds, C
8-10In the aliphatic alcohols compound one or more.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.
Bactericidal composition of the present invention can be used for preventing and treating the disease on the various plants such as vegetables, fruit tree, cereal, ornamental plants, such as early blight and late blight, alternaria leaf spot of apple, downy mildew of garpe, pear scab, wheat rust, melon anthracnose and downy mildew, rice sheath blight disease and the rice blast etc. of the control early blight of tomato and late blight, potato, control efficiency is excellent.
In addition, bactericidal composition of the present invention is compared other similar medicaments also a lot of obviously advantages, and the first, it is more lasting to compare the single dose drug effect, and quick-acting is faster; The second, delayed the drug-fast generation of crop, reduced the number of times of agricultural chemicals Reusability, reduced the agricultural chemicals usage amount, reduce agricultural cost; The 3rd, the residual quantity of this bactericidal composition is lower, and is more friendly to environment; In the application of this bactericidal composition crops there is general fresh-keeping production-increasing function at last, the increasing both production and income of agricultural region is significant.
Embodiment:
In order to make purpose of the present invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention only limits to absolutely not these examples.The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio of below mentioning (comprising percentage) all is mass ratio.Should be pointed out that all any modifications of making within the spirit and principles in the present invention, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Indoor bioassay:
(1) experimental subjects:
The citrus anthracnose germ, the tangerine produced in fujian province blade anthrax scab of adopting back from the Jian Xin of Fuzhou City town separates acquisition, and behind pure culture and Pathogenicity, germ is being added 0.2gmL
-1The tangerine leaf is fried in shallow oil on the PDA medium of juice and is cultivated the conidium 0.1gml of generation
-1G/W is made into 6 * 10
5-9 * 10
5Individual ml
-1Spore suspension
For the examination medium
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO
40.25g/L, NaNO
36g/L, KCL0.5g/L, KH
2PO
41.5g/L, agar powder 20g/L, deionized water 1L
(2) experimental pharmacy:
The former medicine of 95% cyflufenamid, Tso Tat Co., Ltd., Japan, the former medicine of 96% mancozeb, Limin Chemical Co., Ltd..
Combination setting is mixed:
22% cyflufenamid zineb mass ratio was respectively 4: 18,6: 16,8: 14,10: 12,12: 10, i.e. 4%+18%, 6%+16%, 8%+14%, 10%+12%, 12%+10% totally 5 combinations that are mixed.
(3) test method:
1. single dose is to the toxicity test of anthrax bacteria:
Cyflufenamid and mancozeb dissolve with proper amount of acetone, are made into the mother liquor of 10000 μ g/ml, preserve in 4 ℃ of lower low temperature, and be stand-by, is diluted to a series of concentration with sterile water before the test.
The citrus anthracnose germ after preculture on the AEA medium, is inoculated in the bacterium dish of diameter 5mm respectively on the AEA flat board that contains the variable concentrations gradient, under 25 ℃ of dark conditions, cultivates.Every processing repeats 3 times.When control strain colony diameter length is above to 6.5cm, adopt " right-angled intersection method " to measure.According to the linear regression analysis between the logarithm value of the probit value of inhibiting rate and medicine series concentration, obtain the EC that two kinds of single doses suppress mycelial growths
50Value, correlation coefficient and virulence regression equation.
On the basis of preliminary experiment, respectively single dose cyflufenamid and mancozeb are carried out toxicity test, EC in order to upper method
50Value is followed successively by 3.36mg/l and 8.12mg/l.
2. mixture toxicity test:
Carry out the toxicity test of mixture according to mixed ratio with single dose toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun processed
50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single dose toxicity index=(standard medicament EC
50/ reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ reagent agent (using with) EC
50) * 100
The content (%) of toxicity index * B in using with of the content (%) of toxicity index * A of TII=A in using with+B
CTC＝(ATI/TTI)×100
If co-toxicity coefficient is large 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as summation action
(4), experimental result and analysis:
The composite co-toxicity to the citrus anthracnose bacterium of table 1 cyflufenamid and mancozeb
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| Cyflufenamid (A) | Y＝3.0134X+3.4139 | 0.9903 | 3.36 |
| Mancozeb (B) | Y＝2.9475X+2.3191 | 0.9742 | 8.12 |
| A+B is 4: 18 | Y＝2.8133X+3.0719 | 0.9611 | 4.85 |
| A+B is 6: 16 | Y＝3.1167X+3.1388 | 0.9746 | 3.96 |
| A+B is 8: 14 | Y＝2.7954X+3.3939 | 0.9854 | 3.75 |
| A+B is 10: 12 | Y＝3.1821X+3.2213 | 0.9632 | 3.62 |
| A+B is 12: 10 | Y＝2.6649X+3.4543 | 0.9557 | 3.50 |
The composite co-toxicity to citrus anthracnose of table 2 cyflufenamid and mancozeb
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyflufenamid (A) | 1∶0 | 100.00 | 100.00 | / | / |
| Mancozeb (B) | 0∶1 | 41.38 | 41.38 | / | / |
| A+B | 4∶18 | 69.35 | 52.04 | 133.26 | Synergy |
| A+B | 6∶16 | 84.95 | 57.37 | 148.09 | Synergy |
| A+B | 8∶14 | 89.50 | 62.70 | 142.75 | Synergy |
| A+B | 10∶12 | 92.77 | 68.03 | 136.37 | Synergy |
| A+B | 12∶10 | 96.03 | 73.35 | 130.91 | Synergy |
According to bactericide joint toxicity measuring result, cyflufenamid and mancozeb mass ratio are that 6: 16 o'clock co-toxicity coefficient is maximum, are 148.09, so we think that cyflufenamid and 6: 16 o'clock synergistic effect of mancozeb mass ratio are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 6: 16 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Biologicall test example 2: the composite Toxicity Determination to the cucumber downy mildew germ of cyflufenamid and Propineb.
The former medicine of 89% Propineb, Limin Chemical Co., Ltd..
Combination setting is mixed:
25% cyflufenamid Propineb mass ratio was respectively 5: 20,7: 18,9: 16,11: 14,13: 12, i.e. 5%+20%, 7%+18%, 9%+16%, 11%+14%, 13%+12% totally 5 combinations that are mixed.
On the basis of preliminary experiment, respectively single dose cyflufenamid and Propineb are carried out toxicity test, EC in order to upper method
50Value is followed successively by 4.03mg/l and 8.75mg/l.
Test method is the same
Results and analysis:
The composite co-toxicity to the cucumber downy mildew germ of table 3 cyflufenamid and Propineb
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| Cyflufenamid (A) | Y＝2.6635X+3.3877 | 0.9837 | 4.03 |
| Propineb (B) | Y＝2.5749X+25744 | 0.9746 | 8.75 |
| A+B is 5: 20 | Y＝2.4108X+3.3896 | 0.9902 | 4.66 |
| A+B is 7: 18 | Y＝2.7324X+3.3055 | 0.9865 | 4.17 |
| A+B is 9: 16 | Y＝2.4822X+34587 | 0.9689 | 4.18 |
| A+B is 11: 14 | Y＝2.5133X+34537 | 0.9523 | 4.12 |
| A+B is 13: 12 | Y＝2.3902X+3.5662 | 0.9741 | 3.98 |
The composite co-toxicity to the cucumber downy mildew germ of table 4 cyflufenamid and Propineb
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyflufenamid (A) | 1∶0 | 100.00 | 100.00 | / | / |
| Propineb (B) | 0∶1 | 46.06 | 46.06 | / | |
| A+B | 5∶20 | 86.56 | 56.85 | 152.27 | Synergy |
| A+B | 7∶18 | 96.64 | 61.16 | 158.01 | Synergy |
| A+B | 9∶16 | 96.47 | 65.48 | 147.33 | Synergy |
| A+B | 11∶14 | 97.74 | 69.79 | 140.05 | Synergy |
| A+B | 13∶12 | 101.26 | 74.11 | 136.64 | Synergy |
According to bactericide joint toxicity measuring result, cyflufenamid and Propineb mass ratio are that 7: 18 o'clock co-toxicity coefficient is maximum, are 158.01, think that 7: 18 o'clock synergistic effects of cyflufenamid and Propineb mass ratio are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 18 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Biologicall test example 3: the composite Toxicity Determination to the cucumber downy mildew germ of cyflufenamid and Carbatene.
The former medicine of 85% Carbatene, BASF European Co.,Ltd.
Combination setting is mixed:
26% cyflufenamid Carbatene mass ratio was respectively 5: 21,7: 19,9: 17,11: 15,13: 13, i.e. 5%+21%, 7%+19%, 9%+17%, 11%+15%, 13%+13% totally 5 combinations that are mixed.
On the basis of preliminary experiment, respectively single dose cyflufenamid and Carbatene are carried out toxicity test, EC in order to upper method
50Value is followed successively by 4.36mg/l and 7.86mg/l.
Test method is with embodiment 1
Results and analysis:
The composite co-toxicity to the cucumber downy mildew germ of table 5 cyflufenamid and Carbatene
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| Cyflufenamid (A) | Y＝1.8527X+3.8152 | 0.9813 | 4.36 |
| Carbatene (B) | Y＝1.7966X+3.3913 | 0.9601 | 7.86 |
| A+B is 5: 21 | Y＝1.6003X+3.9921 | 0.9745 | 4.26 |
| A+B is 7: 19 | Y＝1.9244X+3.8726 | 0.9802 | 3.85 |
| A+B is 9: 17 | Y＝1.801X+3.9223 | 0.9696 | 3.97 |
| A+B is 11: 15 | Y＝1.7465X+3.9569 | 0.9732 | 3.96 |
| A+B is 13: 13 | Y＝1.6609X+3.5594 | 0.9824 | 4.01 |
The composite co-toxicity to the cucumber downy mildew germ of table 6 cyflufenamid and Carbatene
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyflufenamid (A) | 1∶0 | 100.00 | 100.00 | / | / |
| Carbatene (B) | 0∶1 | 55.47 | 55.47 | / | / |
| A+B | 5∶21 | 102.25 | 64.03 | 159.68 | Synergy |
| A+B | 7∶19 | 113.16 | 67.46 | 167.74 | Synergy |
| A+B | 9∶17 | 109.92 | 70.88 | 155.07 | Synergy |
| A+B | 11∶15 | 110.22 | 74.31 | 148.32 | Synergy |
| A+B | 13∶13 | 108.84 | 77.74 | 140.01 | Synergy |
According to bactericide joint toxicity measuring result, cyflufenamid and Carbatene mass ratio are that 7: 19 o'clock co-toxicity coefficient is maximum, are 167.74, and we think that cyflufenamid and 7: 19 o'clock synergistic effects of Carbatene mass ratio are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 19 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Biologicall test example 4: the composite Toxicity Determination to the powdery mildew of cucumber germ of cyflufenamid and ziram.
The former medicine of 95% ziram, the prompt health chemicals in Tianjin Co., Ltd.
Combination setting is mixed:
20% cyflufenamid ziram mass ratio was respectively 4: 16,6: 14,8: 12,10: 10,12: 8, i.e. 4%+16%, 6%+14%, 8%+12%, 10%+10%, 12%+8% totally 5 combinations that are mixed.
On the basis of preliminary experiment, respectively single dose cyflufenamid and ziram are carried out toxicity test, EC in order to upper method
50Value is followed successively by 3.74mg/l and 7.51mg/l.
Test method is with embodiment 1
Experimental result and analysis:
The composite virulence to the powdery mildew of cucumber germ of table 7 cyflufenamid and ziram
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| Cyflufenamid (A) | Y＝2.8004X+3.3957 | 0.9653 | 3.74 |
| Ziram (B) | Y＝2.7963X+2.5514 | 0.9749 | 7.51 |
| A+B is 4: 16 | Y＝2.6418X+3.3133 | 0.9801 | 4.35 |
| A+B is 6: 14 | Y＝2.8027X+3.4014 | 0.9716 | 3.72 |
| A+B is 8: 12 | Y＝2.9034X+3.3724 | 0.9638 | 3.64 |
| A+B is 10: 10 | Y＝2.7422X+3.4799 | 0.9552 | 3.58 |
| A+B is 12: 8 | Y＝2.8641X+3.4179 | 0.9747 | 3.57 |
The composite co-toxicity to the powdery mildew of cucumber germ of table 8 cyflufenamid and ziram
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyflufenamid (A) | 1∶0 | 100.00 | 100.00 | / | / |
| Ziram (B) | 0∶1 | 49.80 | 49.80 | / | / |
| A+B | 4∶16 | 85.98 | 59.84 | 143.69 | Synergy |
| A+B | 6∶14 | 100.58 | 64.86 | 155.07 | Synergy |
| A+B | 8∶12 | 102.88 | 69.88 | 147.22 | Synergy |
| A+B | 10∶10 | 104.37 | 74.90 | 139.34 | Synergy |
| A+B | 12∶8 | 104.83 | 79.92 | 131.17 | Synergy |
According to bactericide joint toxicity measuring result, cyflufenamid and ziram mass ratio are that 6: 14 o'clock co-toxicity coefficient is maximum, are 155.07, and we think that cyflufenamid and 6: 14 o'clock synergistic effects of ziram mass ratio are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 6: 14 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Field trial 1: to the preventive effect of citrus anthracnose
(1) investigation method
Experimental field be located at tangerine produced in fujian province garden, Xian Nanyu town, the south of Fujian Province and carry out, test tangerine 9a age, anthracnose is medium to lay particular stress on generation; Spray with workers and peasants' 16 type sprayers by design dosage, every residential quarter medication 10L, every 10d medication 1 time shares medicine 2 times.
Come classification according to the citrus anthracnose grade scale, record total strain number, diseased plant number, severity, calculate diseased plant rate, disease index and preventive effect.The citrus anthracnose grade scale is
0 grade: without scab;
1 grade: lesion area accounts for below 10% of whole leaf area;
3 grades: lesion area accounts for the 11%-25% of whole leaf area;
5 grades: lesion area accounts for the 26%-40% of whole leaf area;
7 grades: lesion area accounts for the 41%-65% of whole leaf area;
9 grades: lesion area accounts for more than 65% of whole leaf area.
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 7 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 9 is respectively processed the effect of chemical control citrus anthracnose
Interpretation of result:
As shown in Table 9, the effect of 6: 16 the combination control citrus anthracnose that is mixed of 22% cyflufenamid mancozeb is more remarkable.Behind the medicine 10 days for the first time, for the second time 10 days and 20 days preventive effects 88.05% the highest, 92.21% and 93.19% behind the medicine, single dose 25% cyflufenamid SC for the second time behind the medicine 20 days preventive effects the highest be 83.17%, 80% mancozeb WP for the second time behind the medicine 20 days preventive effect be up to 81.94%.The effect of complex preparation significantly is better than two kinds of single doses, has not only significantly improved the control efficiency for citrus anthracnose, and has further prolonged its lasting period.Simultaneously, under same dosage, 25% cyflufenamid SC single dose is better than 80% mancozeb single dose WP for the control efficiency of anthracnose.
Field trial 2: to the preventive effect of cucumber downy mildew
(1) investigation method
Experimental field be located at Shouguang, Shandong, select the medium cucumber field of occurring degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt the bilinear sampling method, 5 points are got in every residential quarter, look into 5 caves at every, and 2 blades in the fixing middle part of every strain fix a point to check during investigation.
Come classification according to the cucumber downy mildew grade scale, record total strain number, diseased plant number, severity, calculate diseased plant rate, disease index and preventive effect.The cucumber downy mildew grade scale is:
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 10 is respectively organized the test effect that test medicine is processed the control cucumber downy mildew
Interpretation of result:
Can be known that by table 10 effect of 7: 18 the combination control cucumber downy mildew that is mixed of 25% cyflufenamid cyprodinil is very remarkable.Under same 10 milliliters every mu consumption, the effect of complex preparation significantly is better than two kinds of single doses, and its quick-acting and lasting effect are all relatively good, and is especially outstanding with lasting effect.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.20 days preventive effects reach 92.74% after 10 days best control efficiency behind the 1st, 2 medicine reaches respectively 86.55% and 91.44%, the 2 medicine.And single dose 25% cyflufenamid SC, 10 days the highest preventive effect is 85.18% behind the 2nd medicine, 20 days preventive effects are 84.46% behind the 2nd medicine of single dose 70% Propineb WP, the effect of complex preparation significantly is better than two kinds of single doses, not only significantly improve the effect for the cucumber downy mildew germ, and further prolonged its lasting period.
In sum, one of any in cyflufenamid and zineb, Propineb, Carbatene, mancozeb, the ziram have better control efficiency to diseases such as the powdery mildew of various crop, rust, downy mildew, epidemic disease, leaf spot, anthracnose, black spot of fruit tree, spot defoliation, shot holes simultaneously as two yuan of composite preparations of active ingredient, can effectively suppress further developing of disease, simultaneously to crop safety, suggestion is promoted the use of, in use should be used alternatingly with other different mechanisms medicines, to delay the pesticide resistance of plant.
Claims (2)
1. bactericidal composition that contains cyflufenamid, it is characterized in that: active ingredient is (A) cyflufenamid and (B) any one active component binary built in Propineb, Carbatene, mancozeb or the ziram, wherein 22% cyflufenamid. the mancozeb mass ratio was respectively 4: 18,6: 16,8: 14,10: 12,12: 10; 26% cyflufenamid. the Carbatene mass ratio was respectively 5: 21,7: 19,9: 17,11: 15,13: 13; 25% cyflufenamid. the Propineb mass ratio was respectively 5: 20,7: 18,9: 16,11: 14,13: 12; 20% cyflufenamid. the ziram mass ratio was respectively 4: 16,6: 14,8: 12,10: 10,12: 8.
2. bactericidal composition according to claim 1, it is characterized in that: the formulations of pesticide that this bactericidal composition can be mixed with are suspending agent, wetting powder, water dispersible granules, aqueous emulsion, microemulsion.
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| CN103190421A (en) * | 2013-04-07 | 2013-07-10 | 海南正业中农高科股份有限公司 | Cyflufenamid-containing sterilizing composite |
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| CN103385245B (en) * | 2012-05-11 | 2016-05-18 | 陕西韦尔奇作物保护有限公司 | A kind of bactericidal composition containing tiadinil and thiocarbamates |
| CN106857515A (en) * | 2017-04-17 | 2017-06-20 | 北京科发伟业农药技术中心 | Bactericidal composition containing cyflufenamid |
| CN106857593A (en) * | 2017-04-17 | 2017-06-20 | 北京科发伟业农药技术中心 | The Fungicidal composition that cyflufenamid goes out with downy mildew |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1170404A (en) * | 1994-12-19 | 1998-01-14 | 日本曹达株式会社 | Benzamidoxime derivative, process for production thereof, and agrohoricultural bactericide |
| CN102197816A (en) * | 2010-03-24 | 2011-09-28 | 青岛奥迪斯生物科技有限公司 | Efficient bactericidal composition containing cyflufenamid and myclobutanil |
-
2011
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1170404A (en) * | 1994-12-19 | 1998-01-14 | 日本曹达株式会社 | Benzamidoxime derivative, process for production thereof, and agrohoricultural bactericide |
| CN102197816A (en) * | 2010-03-24 | 2011-09-28 | 青岛奥迪斯生物科技有限公司 | Efficient bactericidal composition containing cyflufenamid and myclobutanil |
Non-Patent Citations (2)
| Title |
|---|
| 新型白粉病杀菌剂环氟菌胺;马韵升等;《农药》;20050331;第44卷(第3期);128-129 * |
| 马韵升等.新型白粉病杀菌剂环氟菌胺.《农药》.2005,第44卷(第3期),128-129. |
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| CN103190421A (en) * | 2013-04-07 | 2013-07-10 | 海南正业中农高科股份有限公司 | Cyflufenamid-containing sterilizing composite |
| CN103190421B (en) * | 2013-04-07 | 2014-06-11 | 海南正业中农高科股份有限公司 | Cyflufenamid-containing sterilizing composite |
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