CN102302032A - Novel environment-friendly bactericidal composition - Google Patents
Novel environment-friendly bactericidal composition Download PDFInfo
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- CN102302032A CN102302032A CN201110250142A CN201110250142A CN102302032A CN 102302032 A CN102302032 A CN 102302032A CN 201110250142 A CN201110250142 A CN 201110250142A CN 201110250142 A CN201110250142 A CN 201110250142A CN 102302032 A CN102302032 A CN 102302032A
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Abstract
The invention relates to a bactericidal composition preparation which belongs to the field of the compounding of pesticides. The preparation is obtained by the binary blending of cyflufenamid and partial benzimidazole bactericides which are taken as active ingredients, wherein the weight ratio of the cyflufenamid to the partial benzimidazole bactericides is (80:1)-(1:90), (50:3)-(5:65) preferably; and the total weight content of the active ingredients in the bactericidal composition is 1 to 90 percent, 8 to 75 percent preferably. The balance is auxiliary ingredients which are allowed and acceptable in the pesticides. The preparation can be prepared into wettable powder, a suspending agent, soluble liquid, emulsion in water and water dispersible granules by a known method. The bactericidal composition is mainly used for controlling fungal diseases of crops such as grains, cotton, oil, fruits, vegetables, flowers and the like.
Description
Technical field
The present invention relates to the agriculture chemical compounding technical field, be specifically related to a kind of with one of any in cyflufenamid and part benzimidazoles compound benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol be two yuan of composite bactericidal compositions of active ingredient.
Background technology
Cyflufenamid is the bactericide with novel precursor structure of Japanese Cao Da company exploitation, and its chemical structural formula is following:
It has good effect and characteristic to powdery mildew, the peach grey scab bacterium of various vegetables and wheat class, simultaneously benzimidazole, imidazoles, methoxy acrylate class resistance bacterium and sensitive bacteria is had same isoreactivity.Cyflufenamid is extremely low to people and animals' toxicity, do not exist follow-on influence, teratogenesis, mutagenicity test all is negative, on producing also not to the stimulation and the allergy of eye, skin.In addition, this agent can not influence environment and beneficial organism yet, and is minimum to honeybee and silkworm influence yet.
Benomyl, chemical name: 1-n-butyl amine base formyl-methyl 2-benzimidazolecarbamate, be systemic fungicide, have protection simultaneously, root out and therapeutic action.Fungus-caused disease to Ascomycetes, deuteromycetes and some Basidiomycetes on cereal, grape, a kind of fruit, such as apple, pear, etc. and drupe class, paddy rice and the vegetables has preventive effect.Also can be used for preventing and treating the mite class, mainly as ovicide.Be used for before the results and spraying of results back and dipping, can prevent rotting of fruit and vegetables.
Carbendazim is the high-efficiency low-toxicity systemic fungicide, and interior suction, treatment and protective effect are arranged, and can transfer to other positions by plant absorbing and through conduction, disturbs the mitosis of germ cell, suppresses its growth.Its fungicidal spectrum is wider, be processed to pulvis, wetting powder and suspending agent usually and use, as seed treatment or foliage spray, be used to prevent and treat grain, cotton, oil, really, the multiple fungal diseases of vegetables, flowers, also can be used for the fresh-keeping of fruit.
Thiophanate-methyl is a kind of interior suction low toxicity bactericide of wide spectrum, has interior suction, prevention and therapeutic action, can prevent and treat the various crop disease.It can be converted into carbendazim in plant corpus, the formation of spindle influences cell division in the mitosis of interference bacterium.Applicable crops such as wheat class, paddy rice, cotton, rape, sweet potato, beet, vegetables, flowers etc.Controlling object is stinking smut, head blight for example; Rice blast, banded sclerotial blight; Sclerotinia rot of colza; Cotton disease; Sweet potato black rot; Cucurbits powdery mildew, anthracnose, gray mold; The Kidney bean gray mold; Powdery mildew of pea, brown spot; Floral crops diseases.
Probenazole is a kind of efficient, wide spectrum, general bactericide in the world, inhales to the top conductive performance in having, but can not conduct to base.Lasting period is long, with benzimidazole germicide cross resistance is arranged.Sac fungi, basidiomycetes and imperfect fungus there are the activity of inhibition, are used to prevent and treat various crop fungal disease and antiseptic preservation of fruits and vegetables.
Furidazol is a kind of broad spectrum activity systemic fungicide, is mainly used in seed treatment, diseases such as wheat class snow mold, the bunt of wheat and barley bar disease such as the microbial wheat of control reaping hook, oat.
Benzimidazole germicide through using separately for a long time, has produced very big pesticide resistance, causes dosage to strengthen, and problems such as lasting period shortening are not unfavorable for the sustainable development of environment very much.But benzimidazole germicide, interior absorption is better, and has therapeutic action concurrently, at pesticide field, sees that from the mode of action and cost cost performance is higher, still has bigger room for improvement.I research staff of company attempts with the new type bactericide of different effects mechanism composite with it; To improve its preventive effect; Reduce its agricultural cost,, in indoor prescription screening, chance on through test of indoor virulence repeatedly and field experiment; Composite new type bactericide cyflufenamid and the above part benzimidazole germicide; Synergistic effect is obvious, can obviously improve preventive effect, reduces the dosage of agricultural chemicals; Also can enlarge fungicidal spectrum, improve germicidal efficiency.And, do not see relevant report at present at home and abroad as yet about the research in new type bactericide cyflufenamid and the composite field of above-mentioned benzimidazoles compound.
Summary of the invention
I research staff of company is devoted for years in the research in the composite field of agriculture chemicals fungicide; Through a large amount of formula test screenings; A kind of new and effective bactericidal composition has been proposed; Bactericidal composition of the present invention is with new type bactericide cyflufenamid and part benzimidazoles compound benomyl; Carbendazim; Thiophanate-methyl; Probenazole; One of any two yuan of carrying out for active ingredient are mixed in the furidazol; Can effectively solve the powdery mildew that is used to prevent and treat various crop; Rust; Downy mildew; Eqpidemic disease; Leaf spot; Disease such as anthracnose and rice blast; Can obviously improve actual preventive effect; Delay the drug-fast generation of germ, reduce drug cost.
The present invention is a kind of with new type bactericide cyflufenamid and part benzimidazole germicide benomyl; Carbendazim; Thiophanate-methyl; Probenazole; Carry out two yuan of bactericidal composition preparations that are mixed for active ingredient one of arbitrarily in the furidazol; Active ingredient new type bactericide cyflufenamid and part benzimidazole germicide benomyl in the bactericidal composition wherein of the present invention; Carbendazim; Thiophanate-methyl; Probenazole; Percentage by weight one of any in the furidazol is 80: 1～1: 90; On the basis of test recipe screening targetedly, can be with active ingredient new type bactericide cyflufenamid and part benzimidazole germicide benomyl; Carbendazim; Thiophanate-methyl; Probenazole; Percentage by weight one of any in the furidazol is preferably 50: 3～and 5: 65.
Part benzimidazole germicide compound wherein of the present invention is specially in benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol one of any.
The formulations of pesticide that pesticide sterilizing composite of the present invention uses the preparation method in pesticidal preparations field to be prepared into are wetting powder, suspending agent, soluble liquid, aqueous emulsion and water dispersible granules; The gross mass of active ingredient accounts for 1%～90% of preparation gross mass in the preparation; Be preferably 8%～75%, all the other are usual auxiliaries in the agricultural chemicals.
Pesticide sterilizing composite of the present invention to grain, cotton, oil, really, the multiple fungal diseases of vegetables, flowers all has favorable effect, especially diseases such as powdery mildew, leaf spot, rust, downy mildew, eqpidemic disease, anthracnose and scab had excellent preventive effect.For example prevent and treat powdery mildew of wheat and barley; Sclerotinia rot of colza; Cotton disease; Sweet potato black rot; Cucurbits powdery mildew, anthracnose, gray mold; The Kidney bean gray mold; Powdery mildew of pea, brown spot; Floral crops diseases, downy mildew of garpe, wheat rust, melon anthracnose and downy mildew etc.
In addition, bactericidal composition of the present invention is compared similar medicament also has a lot of other obvious advantages, and the first, it is more lasting to compare the single-dose effect, and quick-acting also has raising; The second, delayed the drug-fast generation of crop greatly, reduced agricultural chemicals access times repeatedly, reduced the agricultural chemicals usage amount, reduce agricultural cost; The 3rd, the residual quantity of this bactericidal composition is lower, and is friendly more to environment; In the application of this bactericidal composition crops there is general fresh-keeping production-increasing function at last, the increasing both production and income of agricultural region is significant.
Embodiment:
In order to make the object of the invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention only limits to these examples absolutely not.The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio of below mentioning (comprising percentage) all is a mass ratio.Should be pointed out that all any modifications of within spirit of the present invention and principle, being made, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with accompanying claims.
Wherein prepare required necessary basis component about the formulations of pesticide and the easy manufacture implementation method following:
Cyflufenamid is represented with active principle A in following implementer's case, and active principle B represents in part benzimidazole germicide compound benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol one of any.
One of technical scheme of the present invention, described bactericidal composition are wetting powder, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition wetting powder is: press above-mentioned prescription with active principle A and active principle B and dispersant, wetting agent and filler mixing; In stirred tank, evenly stir; Behind airslide disintegrating mill, mixing, promptly can be made into the wetting powder of the present composition.
Two of technical scheme of the present invention, described bactericidal composition are suspending agent, and the percentage by weight of component is:
The concrete production stage of this suspending agent is earlier other auxiliary agents to be mixed; Mix through high speed shear, add active principle A and active principle B, abrading-ball is 2～3 hours in ball crusher; Make a diameter all below 5mm, make the suspending agent preparation of bactericidal composition of the present invention.
Three of technical scheme of the present invention, described bactericidal composition are aqueous emulsion, and the percentage by weight of component is:
The concrete production stage of this aqueous emulsion is: at first former medicine active principle A is added with active principle B, solvent and emulsifier, cosolvent and be in the same place, make and be dissolved into uniform oil phase; With part water, antifreeze, other insecticides adjuvant such as antimicrobial mixes into uniform water; When the agitated reactor high speed stirs, oil phase is added water, slowly add water, open clipper and carry out high speed shear, and add remaining water, shear half an hour approximately, form the aqueous emulsion of oil-in-water type until reaching the phase inversion point.Promptly make the aqueous emulsion of bactericidal composition of the present invention.
Four of technical scheme of the present invention, described bactericidal composition are water dispersible granules, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition water dispersible granules is: by above-mentioned prescription active principle A, active principle B and dispersant, wetting agent, disintegrant and filler are mixed; Pulverize with micro jet; Through mediating; Add then and carry out granulation, drying, screening in the fluidized bed prilling dryer after sample analysis, promptly make the water dispersible granules of bactericidal composition of the present invention.
Described emulsifier is selected from any one or more mixtures formed with arbitrary proportion in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, alkylphenol polyoxyethylene sulfosuccinate, styryl phenol APEO, polyoxyethylene nonylphenol ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, the polyoxyethylene aliphatic alcohol ether.
Said solvent is any one or more mixed solvents formed with arbitrary proportion in xylol or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), the water equal solvent.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, the fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, draw back in powder copper rosinate X, wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, the soapberry powder one or more.
Described disintegrant is selected from: one or more in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, the sodium bicarbonate.
Described thickener is selected from: one or more in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol.
Described stabilizing agent is selected from: a kind of in sodium citrate, the resorcinol.
Described antifreezing agent is selected from: one or more in ethylene glycol, propane diols, the glycerine.
Described defoamer is selected from: silicone oil, silicone compound, C
10-20Saturated fat acid compounds, C
8-10In the aliphatic alcohols compound one or more.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.
Indoor virulence test and field experiment
The indoor virulence measuring:
Biologicall test instance 1:
(1) experimental subjects:
The powdery mildew of cucumber germ, separation and purification gained from the powdery mildew of cucumber incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Supply the examination medium:
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO
40.25g/L, NaNO
36g/L, KCl0.5g/L, KH
2PO
41.5g/L, agar powder 20g/L, deionized water 1L.
(2) experimental pharmacy:
The former medicine of 95% cyflufenamid, Tso Tat Co., Ltd., Japan, 98% carbendazim active compound, Jiangsu Yangnong Chemical Group Co., Ltd.
Combination setting is mixed:
17% cyflufenamid carbendazim percentage composition was respectively 3: 14,5: 12,7: 10,9: 8,11: 6, i.e. 3%+14%, 5%+12%, 7%+10%, 9%+8%, 11%+6% totally 5 combinations that are mixed.
(3) test method:
Single agent is to the toxicity test of powdery mildew of cucumber germ:
Cyflufenamid and carbendazim dissolve with proper amount of acetone, are made into the mother liquor of 10000 μ g/ml, preserve in 4 ℃ of following low temperature, and be for use, is diluted to a series of concentration with sterile water before the test.
The powdery mildew of cucumber germ after cultivating in advance on the AEA medium, is inoculated in the bacterium dish of diameter 5mm respectively on the AEA flat board that contains the variable concentrations gradient, cultivates down in 25 ℃ of dark conditions.Every processing repetition 3 times.When control strain colony diameter length is above to 6.5cm, adopt " right-angled intersection method " to measure.According to the linear regression analysis between the logarithm value of the probit value of inhibiting rate and medicine series concentration, obtain the EC that two kinds of single agent suppress mycelial growths
50Value, correlation coefficient and virulence regression equation.
On the basis of preliminary experiment, respectively single agent cyflufenamid and carbendazim are carried out toxicity test, EC in order to last method
50Value is followed successively by 4.53mg/l and 8.65mg/l.
The mixture toxicity test:
Carry out the toxicity test of mixture according to mixed ratio with single agent toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun handled
50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single agent toxicity index=(standard medicament EC
50/ confession reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ confession reagent agent (using with) EC
50) * 100
The content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A of TII=A in using with
CTC＝(ATI/TTI)×100
If co-toxicity coefficient is greater than 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as summation action.
(4) result and analysis:
The composite co-toxicity test of table 1 cyflufenamid and carbendazim to the powdery mildew of cucumber germ
The composite co-toxicity test of table 2 cyflufenamid and carbendazim to the powdery mildew of cucumber germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyflufenamid (A) | 1∶0 | 100.00 | 100.00 | / | / |
| Carbendazim (B) | 0∶1 | 52.37 | 52.37 | / | / |
| A∶B | 3∶14 | 92.03 | 60.78 | 151.43 | Synergy |
| A∶B | 5∶12 | 117.34 | 66.38 | 176.78 | Synergy |
| A∶B | 7∶10 | 118.83 | 71.98 | 165.08 | Synergy |
| A∶B | 9∶8 | 119.74 | 77.59 | 154.33 | Synergy |
| A∶B | 11∶6 | 120.00 | 83.19 | 144.25 | Synergy |
Measuring the result according to above indoor virulence test shows; Cyflufenamid and carbendazim all have synergistic effect preferably by the percentage composition of experiment; Wherein with cyflufenamid: carbendazim is that 5: 12 o'clock co-toxicity coefficient is up to 176.78, thinks that cyflufenamid and carbendazim percentage composition are that 5: 12 o'clock synergistic effects are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 5: 12 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Biologicall test instance 2: the composite Toxicity Determination of cyflufenamid and thiophanate-methyl to the powdery mildew of cucumber germ.
The former medicine of 95% thiophanate-methyl, Jiangsu lark farmingization Co., Ltd.
The powdery mildew of cucumber germ, separation and purification gained from the powdery mildew of cucumber incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent cyflufenamid and thiophanate-methyl are carried out toxicity test, EC
50Value is followed successively by 4.18mg/l and 7.89mg/l.
Combination setting is mixed:
20% cyflufenamid thiophanate-methyl percentage composition was respectively 3: 17,5: 15,7: 13,9: 11,11: 9, i.e. 3%+17%, 5%+15%, 7%+13%, 9%+11%, 11%+9% totally 5 combinations that are mixed.
Test method is with biologicall test embodiment 1
Experimental result and analysis:
The composite virulence experiment of table 3 cyflufenamid and thiophanate-methyl to the powdery mildew of cucumber germ
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| Cyflufenamid (A) | Y＝1.7359X+3.9217 | 0.9521 | 4.18 |
| Thiophanate-methyl (B) | Y＝1.8027X+3.3828 | 0.9639 | 7.89 |
| A: B is 3: 17 | Y＝1.6439X+3.9644 | 0.9408 | 4.27 |
| A: B is 5: 15 | Y＝1.5863X+4.1249 | 0.9755 | 3.56 |
| A: B is 7: 13 | Y＝1.6107X+4.1426 | 0.9492 | 3.41 |
| A: B is 9: 11 | Y＝1.8003X+3.9836 | 0.9613 | 3.67 |
| A: B is 11: 9 | Y＝1.7724X+4.0077 | 0.9805 | 3.63 |
The composite co-toxicity test of table 4 cyflufenamid and thiophanate-methyl to the powdery mildew of cucumber germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyflufenamid (A) | 1∶0 | 100.00 | 100.00 | / | / |
| Thiophanate-methyl (B) | 0∶1 | 52.98 | 52.98 | / | |
| A∶B | 3∶17 | 98.00 | 60.03 | 163.25 | Synergy |
| A∶B | 5∶15 | 117.37 | 64.73 | 181.31 | Synergy |
| A∶B | 7∶13 | 122.72 | 69.44 | 176.74 | Synergy |
| A∶B | 9∶11 | 113.93 | 74.14 | 153.67 | Synergy |
| A∶B | 11∶9 | 115.18 | 78.84 | 146.09 | Synergy |
Measuring the result according to above indoor virulence test shows; The used cyflufenamid and the percentage composition of thiophanate-methyl all have synergistic effect in the experiment; Wherein the co-toxicity coefficient when the percentage composition of cyflufenamid and thiophanate-methyl is 5: 15 is 181.31 to the maximum, thinks that promptly 5: 15 o'clock synergistic effects of percentage composition of cyflufenamid and thiophanate-methyl are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 5: 15 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Biologicall test instance 3: the composite Toxicity Determination of cyflufenamid and benomyl to pear scab.
The pear scab germ, separation and purification gained from the pear tree incidence of leaf of Laiyang, Shandong is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent cyflufenamid and benomyl are carried out toxicity test, EC
50Value is followed successively by 3.53mg/l and 8.11mg/l.
Combination setting is mixed:
The percentage composition of 21% cyflufenamid benomyl was respectively 3: 18,5: 16,7: 14,9: 12,11: 10, i.e. 3%+18%, 5%+16%, 7%+14%, 9%+12%, 11%+10% totally 5 combinations that are mixed.
Test method is similar with biologicall test embodiment 1
Result and analysis:
The composite co-toxicity of table 5 cyflufenamid and benomyl to the pear scab germ
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| Cyflufenamid (A) | Y＝1.9325X+3.9414 | 0.9499 | 3.53 |
| Benomyl (B) | Y＝1.8533X+3.3153 | 0.9536 | 8.11 |
| A: B is 3: 18 | Y＝1.7821X+3.8007 | 0.9627 | 4.71 |
| A: B is 5: 16 | Y＝1.8426X+3.9525 | 0.9702 | 3.70 |
| A: B is 7: 14 | Y＝1.7328X+4.0303 | 0.9694 | 3.63 |
| A: B is 9: 12 | Y＝1.6434X+4.0879 | 0.9583 | 3.59 |
| A: B is 11: 10 | Y＝1.7111X+4.0711 | 0.9745 | 3.49 |
The composite co-toxicity of table 6 cyflufenamid and benomyl to the pear scab germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Cyflufenamid (A) | 1∶0 | 100.00 | 100.00 | / | / |
| Benomyl (B) | 0∶1 | 43.53 | 43.53 | / | / |
| A∶B | 3∶18 | 74.96 | 51.59 | 145.29 | Synergy |
| A∶B | 5∶16 | 95.34 | 56.97 | 167.35 | Synergy |
| A∶B | 7∶14 | 97.31 | 62.35 | 156.07 | Synergy |
| A∶B | 9∶12 | 98.36 | 67.73 | 145.22 | Synergy |
| A∶B | 11∶10 | 101.14 | 73.11 | 138.34 | Synergy |
Mensuration result according to the test of bactericide co-toxicity; When cyflufenamid and benomyl percentage composition are above experiment ratio; Result of the test shows all has synergistic effect; And the co-toxicity coefficient when ratio is 5: 16 is 167.35 to the maximum; We can think that the percentage composition of cyflufenamid and benomyl is at 5: 16 o'clock, and synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 5: 16 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Field trial 1: to the preventive effect of pear scab
(1) investigation method
Experimental field be located at 5 years living theatre, Laiyang, Shandong and carry out, 4 repetitions are established in every processing, every repetition 10 strains, and randomized arrangement, guard row is established in the centre.Every fixing investigation 4 strains, 10 at every strain investigation spring tip, 60 blades, 30 of young fruits of repeating.April 20, Mays 10 daily adopt knapsack sprayer to spray tree crown, and each sprays medicine once, and the soup consumption is 1201/667m
2
Come classification according to the pear scab grade scale, grade scale does
0 grade: blade does not have scab, young fruit does not have scab;
1 grade: blade 1～5 scab, young fruit have a small amount of scab;
2 grades: 6～15 scabs of blade, young fruit 1/3 fruit face have scab;
3 grades: 16 above scabs of blade, but blade is non-warping, young fruit 1/2 fruit face has scab;
4 grades: the leaf malformation distortion, full fruit has scab;
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 7 is respectively organized the test medicine processing and is prevented and treated the test effect of pear scab
Interpretation of result:
Can know by table 7; The effect that 5: 16 the combination that is mixed of 21% cyflufenamid benomyl prevents and treats pear scab is more remarkable; Behind the medicine 10 days for the first time; For the second time 10 days and 20 days preventive effects 89.24%, 91.32% and 92.41% the highest behind the medicine; 20 days preventive effects the highest are 85.86% behind the single agent 10% cyflufenamid EC medicine second time; 20 days preventive effect is up to 85.58% behind the 50% benomyl WP medicine second time; The effect of complex preparation significantly is better than two kinds of single agent; Not only significantly improved effect, and further prolonged its lasting period for pear scab.
Field trial 2: to the preventive effect of powdery mildew of cucumber
(1) investigation method
Experimental field be located at Shouguang, Shandong, publish the medium cucumber field of course of disease degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt the bilinear sampling method, 5 points are got in every sub-district, look into 5 caves at every, and 2 blades in the fixing middle part of every strain fix a point to check during investigation.
Come classification according to the powdery mildew of cucumber grade scale, write down total strain number, diseased plant number, degree of being in a bad way, calculate diseased plant rate, disease index and preventive effect.The powdery mildew of cucumber grade scale is:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 8 is respectively organized the test medicine processing and is prevented and treated the test effect of powdery mildew of cucumber
Interpretation of result:
Can know that by table 8 effect that 5: 15 the combination that is mixed of 20% cyflufenamid thiophanate-methyl prevents and treats powdery mildew of cucumber is very remarkable.Under same 10 milliliters every mu consumption, the effect of complex preparation significantly is better than two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and is the most outstanding with lasting effect especially.Complex preparation significantly is better than the effect under the low concentration consumption in control efficiency under the consumption of high concentration.20 days preventive effects reach 91.22% after 10 days best control efficiency behind the 1st, 2 medicine reaches 86.32% and 89.64%, the 2 medicine respectively.And single agent 10% cyflufenamid EC; 10 days the highest preventive effect is 82.35% behind the 2nd medicine; 20 days preventive effects are 82.65% behind the 2nd medicine of single agent 70% thiophanate-methyl WP; The effect of complex preparation significantly is better than two kinds of single agent; Not only significantly improved effect, and further prolonged its lasting period for the powdery mildew of cucumber germ.
Comprehensive above experiment is said; With new type bactericide cyflufenamid and part benzimidazole germicide benomyl; Carbendazim; Thiophanate-methyl; Probenazole; Furidazol is that active ingredient is carried out the powdery mildew of two yuan of bactericidal composition preparations that are mixed to various crop; Leaf spot; Rust; Downy mildew; Eqpidemic disease; Anthracnose and drupe are deceived star; Diseases such as grey scab all have the better prevention effect; Can effectively suppress further developing of disease; Simultaneously to crop safety; Suggestion is promoted the use of; In use should be used alternatingly, to delay the pesticide resistance of plant with other different mechanisms medicines.
Claims (5)
1. pesticide sterilizing composite preparation; It is characterized in that: this bactericidal composition be with one of any in A new type bactericide cyflufenamid and B part benzimidazole germicide benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol be the preparation of two yuan of composite bactericidal compositions of active ingredient; All the other components are usual auxiliaries and complementary component in the agricultural chemicals, and wherein the percentage by weight of effective constituents A and B is 80: 1～1: 90 in this bactericidal composition.
2. bactericidal composition preparation according to claim 1 is characterized in that: on the basis of specific aim experimental formula, the percentage by weight of effective constituents A and B can be preferably 50: 3～5: 65 in this bactericidal composition preparation.
3. according to the described bactericidal composition preparation of claim 1 to 2, it is characterized in that: to account for the percentage of gross weight in the preparation be 1%～90% for effective constituents A and B total content in this bactericidal composition preparation.
4. bactericidal composition preparation according to claim 3 is characterized in that: effective constituents A and the B gross weight percentage composition in preparation is preferably 8%～75% in this bactericidal composition preparation.
5. according to the described bactericidal composition preparation of claim 1 to 4, it is characterized in that: the preparation formulation that bactericidal composition preparation of the present invention can be mixed with is wetting powder, suspending agent, soluble liquid, aqueous emulsion and water dispersible granules.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104642368A (en) * | 2013-11-21 | 2015-05-27 | 苏州田园农业技术开发有限公司 | Low-temperature-resistant chilli seed initiator |
| EP3616513A4 (en) * | 2017-04-28 | 2021-01-20 | Nippon Soda Co., Ltd. | Suspoemulsion |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1319341A1 (en) * | 1998-04-30 | 2003-06-18 | Nippon Soda Co., Ltd. | Fungicidal composition for agriculture and horticulture comprising a benzamidoxime compound and a benzimidazole compound |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1319341A1 (en) * | 1998-04-30 | 2003-06-18 | Nippon Soda Co., Ltd. | Fungicidal composition for agriculture and horticulture comprising a benzamidoxime compound and a benzimidazole compound |
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| 马韵升等: "新型白粉病杀菌剂环氟菌胺", 《农药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104642368A (en) * | 2013-11-21 | 2015-05-27 | 苏州田园农业技术开发有限公司 | Low-temperature-resistant chilli seed initiator |
| EP3616513A4 (en) * | 2017-04-28 | 2021-01-20 | Nippon Soda Co., Ltd. | Suspoemulsion |
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