CN102308832A - Novel environmentally friendly antibacterial composition - Google Patents
Novel environmentally friendly antibacterial composition Download PDFInfo
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- CN102308832A CN102308832A CN201110184940A CN201110184940A CN102308832A CN 102308832 A CN102308832 A CN 102308832A CN 201110184940 A CN201110184940 A CN 201110184940A CN 201110184940 A CN201110184940 A CN 201110184940A CN 102308832 A CN102308832 A CN 102308832A
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Abstract
The invention relates to an antibacterial composition in the field of pesticide compounding. Effective components of the antibacterial composition are bixafen and a partial benzimidazole bactericide which are mixed according to a weight ratio of 80:1-1:90, preferably 50:3-5:65. The total weight percentage of the effective components in the antibacterial composition is 1-90%, preferably 8-75%, and the rest is allowably used and pesticide acceptable auxiliary components. A wettable pulvis, a suspending agent, a soluble liquid formulation, an emulsion in water, and a water dispersion granule can be prepared with known methods. The antibacterial composition of the present invention is mainly used in controlling fungal diseases of crops of grains, cotton, oil, fruit, vegetables and flowers.
Description
Technical field
The present invention relates to the agriculture chemical compounding technical field, be specifically related to a kind of bixafen of containing (C
18H
12Cl
2F
3N
3O) one of any and in the part benzimidazoles compound benomyl, carbendazim, thiophanate-methyl, probenazole, furidazol is two yuan of composite bactericidal compositions of active ingredient.
Background technology
Bixafen (C
18H
12Cl
2F
3N
3O), be a kind of novel SDI pyrazoles bactericide, the chemical structural formula of bixafen is:
Bixafen is the succinate dehydrogenase inhibitor; With mainly developing the control that is used for leaf spot and leaf rust; But show through research; This new compound all has excellent activity to multiple germ, like the comparatively general germ of pesticide resistance such as white powder germ, ash arrhizus bacteria, Pseudoperonospora cubensis, is expected to become the important kind of bactericide resistance management.
Benomyl, chemical name: 1-n-butyl amine base formyl-methyl 2-benzimidazolecarbamate, be systemic fungicide, have protection simultaneously, root out and therapeutic action.Fungus-caused disease to Ascomycetes, deuteromycetes and some Basidiomycetes on cereal, grape, a kind of fruit, such as apple, pear, etc. and drupe class, paddy rice and the vegetables has preventive effect.Also can be used for preventing and treating the mite class, mainly as ovicide.Be used for before the results and spraying of results back and dipping, can prevent rotting of fruit and vegetables.
Carbendazim is the high-efficiency low-toxicity systemic fungicide, and interior suction, treatment and protective effect are arranged, and can transfer to other positions by plant absorbing and through conduction, disturbs the mitosis of germ cell, suppresses its growth.Its fungicidal spectrum is wider, be processed to pulvis, wetting powder and suspending agent usually and use, as seed treatment or foliage spray, be used to prevent and treat grain, cotton, oil, really, the multiple fungal diseases of vegetables, flowers, also can be used for the fresh-keeping of fruit.
Thiophanate-methyl is a kind of interior suction low toxicity bactericide of wide spectrum, has interior suction, prevention and therapeutic action, can prevent and treat the various crop disease.It can be converted into carbendazim in plant corpus, the formation of spindle influences cell division in the mitosis of interference bacterium.Applicable crops such as wheat class, paddy rice, cotton, rape, sweet potato, beet, vegetables, flowers etc.Controlling object is stinking smut, head blight for example; Rice blast, banded sclerotial blight; Sclerotinia rot of colza; Cotton disease; Sweet potato black rot; Cucurbits powdery mildew, anthracnose, gray mold; The Kidney bean gray mold; Powdery mildew of pea, brown spot; Floral crops diseases.
Probenazole is a kind of efficient, wide spectrum, general bactericide in the world, inhales to the top conductive performance in having, but can not conduct to base.Lasting period is long, with benzimidazole germicide cross resistance is arranged.Sac fungi, basidiomycetes and imperfect fungus there are the activity of inhibition, are used to prevent and treat various crop fungal disease and antiseptic preservation of fruits and vegetables.
Furidazol is a kind of broad spectrum activity systemic fungicide, is mainly used in seed treatment, diseases such as wheat class snow mold, the bunt of wheat and barley bar disease such as the microbial wheat of control reaping hook, oat.
Benzimidazole germicide through using separately for a long time, has produced very big pesticide resistance, causes dosage to strengthen, and problems such as lasting period shortening are not unfavorable for the sustainable development of environment very much.But benzimidazole germicide, interior absorption is better, and has therapeutic action concurrently, at pesticide field, sees that from the mode of action and cost cost performance is higher, still has bigger room for improvement.I research staff of company attempts with the new type bactericide of different effects mechanism composite with it, to improve its preventive effect, reduces its agricultural cost; Through test of indoor virulence repeatedly and field experiment, in indoor prescription screening, chance on, composite new type bactericide bixafen and the above part benzimidazole germicide; Synergistic effect is obvious, can obviously improve preventive effect, reduces the dosage of agricultural chemicals; Also can enlarge fungicidal spectrum, improve germicidal efficiency.And, do not see relevant report at present at home and abroad as yet about the research in new type bactericide bixafen and the composite field of above-mentioned benzimidazoles compound.
Summary of the invention
I research staff of company is devoted for years in the research in the composite field of agriculture chemicals fungicide; Through a large amount of formula test screenings; A kind of bactericidal composition of new high-efficiency has been proposed; Bactericidal composition of the present invention be with one of any in new type bactericide bixafen and part benzimidazoles compound benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol be that two yuan of carrying out of active ingredient are mixed, be used to prevent and treat the diseases such as powdery mildew, rust, downy mildew, eqpidemic disease, leaf spot, anthracnose and rice blast of various crop, can obviously improve actual preventive effect; Delay the drug-fast generation of germ, reduce drug cost.
The present invention for a kind of with one of any in new type bactericide bixafen and part benzimidazole germicide benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol be that active ingredient is carried out two yuan of bactericidal composition preparations that are mixed; In the bactericidal composition wherein of the present invention in active ingredient new type bactericide bixafen and part benzimidazole germicide benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol one of any percentage by weight be 80: 1～1: 90; On the basis of the screening of test recipe targetedly, can percentage by weight one of any in active ingredient new type bactericide bixafen and part benzimidazole germicide benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol be preferably 50: 3～5: 65.
Part benzimidazole germicide compound wherein of the present invention is specially in benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol one of any.
The formulations of pesticide that pesticide sterilizing composite of the present invention uses the preparation method in pesticidal preparations field to be prepared into are wetting powder, suspending agent, soluble liquid, aqueous emulsion and water dispersible granules; The gross mass of active ingredient accounts for 1%～90% of preparation gross mass in the preparation; Be preferably 8%～75%, all the other are usual auxiliaries in the agricultural chemicals.
Pesticide sterilizing composite of the present invention to grain, cotton, oil, really, the multiple fungal diseases of vegetables, flowers all has favorable effect, especially diseases such as leaf spot, powdery mildew, rust, downy mildew, eqpidemic disease, anthracnose and rice blast had excellent preventive effect.For example prevent and treat stinking smut, head blight; Rice blast, banded sclerotial blight; Sclerotinia rot of colza; Cotton disease; Sweet potato black rot; Cucurbits powdery mildew, anthracnose, gray mold; The Kidney bean gray mold; Powdery mildew of pea, brown spot; Floral crops diseases, downy mildew of garpe, wheat rust, melon anthracnose and downy mildew etc.
In addition, bactericidal composition of the present invention is compared similar medicament also has a lot of other obvious advantages, and the first, it is more lasting to compare the single-dose effect, and quick-acting also has raising; The second, delayed the drug-fast generation of crop greatly, reduced agricultural chemicals access times repeatedly, reduced the agricultural chemicals usage amount, reduce agricultural cost; The 3rd, the residual quantity of this bactericidal composition is lower, and is friendly more to environment; In the application of this bactericidal composition crops there is general fresh-keeping production-increasing function at last, the increasing both production and income of agricultural region is significant.
Embodiment:
In order to make the object of the invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention only limits to these examples absolutely not.The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio of below mentioning (comprising percentage) all is a mass ratio.Should be pointed out that all any modifications of within spirit of the present invention and principle, being made, be equal to replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with accompanying claims.
Wherein prepare required necessary basis component about the formulations of pesticide and the easy manufacture implementation method following:
Bixafen (C in following implementer's case
18H
12Cl
2F
3N
3O) represent with active principle A that active principle B represents in part benzimidazole germicide compound benomyl, carbendazim, thiophanate-methyl, probenazole, the furidazol one of any.
One of technical scheme of the present invention, described bactericidal composition are wetting powder, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition wetting powder is: press above-mentioned prescription with active principle A and active principle B and dispersant, wetting agent and filler mixing; In stirred tank, evenly stir; Behind airslide disintegrating mill, mixing, promptly can be made into the wetting powder of the present composition.
Two of technical scheme of the present invention, described bactericidal composition are suspending agent, and the percentage by weight of component is:
The concrete production stage of this suspending agent is earlier other auxiliary agents to be mixed; Mix through high speed shear, add active principle A and active principle B, abrading-ball is 2～3 hours in ball crusher; Make a diameter all below 5mm, make the suspending agent preparation of bactericidal composition of the present invention.
Three of technical scheme of the present invention, described bactericidal composition are aqueous emulsion, and the percentage by weight of component is:
The concrete production stage of this aqueous emulsion is: at first former medicine active principle A is added with active principle B, solvent and emulsifier, cosolvent and be in the same place, make and be dissolved into uniform oil phase; With part water, antifreeze, other insecticides adjuvant such as antimicrobial mixes into uniform aqueous phase; When the agitated reactor high speed stirs, oil phase is added water, slowly add water, open clipper and carry out high speed shear, and add remaining water, shear half an hour approximately, form the aqueous emulsion of oil-in-water type until reaching the phase inversion point.Promptly make the aqueous emulsion of bactericidal composition of the present invention.
Four of technical scheme of the present invention, described bactericidal composition are water dispersible granules, and the percentage by weight of component is:
The concrete procedure of processing of this bactericidal composition water dispersible granules is: by above-mentioned prescription active principle A, active principle B and dispersant, wetting agent, disintegrant and filler are mixed; Pulverize with micro jet; Through mediating; Add then and carry out granulation, drying, screening in the fluidized bed prilling dryer after sample analysis, promptly make the water dispersible granules of bactericidal composition of the present invention.
Described emulsifier is selected from any one or more mixtures formed with arbitrary proportion in calcium dodecyl benzene sulfonate and aliphatic acid polyethenoxy ether, APES sulfosuccinate, styryl phenol APEO, NPE, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, the polyoxyethylene aliphatic alcohol ether.
Said solvent is any one or more mixed solvents formed with arbitrary proportion in xylol or biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), the water equal solvent.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, APES formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, APES, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, the fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, draw back in powder copper rosinate X., wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, the soapberry powder one or more.
Described disintegrant is selected from: one or more in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, the sodium bicarbonate.
Described thickener is selected from: one or more in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, the polyvinyl alcohol.
Described stabilizing agent is selected from: a kind of in sodium citrate, the resorcinol.
Described antifreezing agent is selected from: one or more in ethylene glycol, propane diols, the glycerine.
Described antifoaming agent is selected from: silicone oil, silicone compound, C
10-20Saturated fat acid compounds, C
8-10In the aliphatic alcohols compound one or more.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.
Indoor virulence test and field experiment
The indoor virulence measuring:
Biologicall test instance 1:
(1) experimental subjects:
The cucumber downy mildew germ, separation and purification gained from the cucumber downy mildew incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Supply the examination medium:
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO
40.25g/L, NaNO
36g/L, KCl0.5g/L, KH
2PO
41.5g/L, agar powder 20g/L, deionized water 1L.
(2) experimental pharmacy:
The former medicine of 90%bixafen, Bayer crop science Co., Ltd, 98% carbendazim active compound, Jiangsu Yangnong Chemical Group Co., Ltd.
Combination setting is mixed:
22%bixafen carbendazim percentage composition was respectively 3: 19,5: 17,7: 15,9: 13,11: 11, i.e. 3%+19%, 5%+17%, 7%+15%, 9%+13%, 11%+11% totally 5 combinations that are mixed.
(3) test method:
Single agent is to the toxicity test of cucumber downy mildew germ:
Bixafen and carbendazim dissolve with proper amount of acetone, are made into the mother liquor of 10000 μ g/ml, preserve in 4 ℃ of following low temperature, and be for use, is diluted to a series of concentration with sterile water before the test.
The cucumber downy mildew germ after cultivating in advance on the AEA medium, is inoculated in the bacterium dish of diameter 5mm respectively on the AEA flat board that contains the variable concentrations gradient, cultivates down in 25 ℃ of dark conditions.Every processing repetition 3 times.When control strain colony diameter length is above to 6.5c m, adopt " right-angled intersection method " to measure.According to the linear regression analysis between the logarithm value of the probit value of inhibiting rate and medicine series concentration, obtain the EC that two kinds of single agent suppress mycelial growths
50Value, correlation coefficient and virulence regression equation.
On the basis of preliminary experiment, respectively single agent bixafen and carbendazim are carried out toxicity test, EC in order to last method
50Value is followed successively by 3.26mg/l and 7.21mg/l.
The mixture toxicity test:
Carry out the toxicity test of mixture according to mixed ratio with single agent toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun handled
50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single agent toxicity index=(standard medicament EC
50/ confession reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ confession reagent agent (using with) EC
50) * 100
The content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A of TII=A in using with
CTC＝(ATI/TTI)×100
If co-toxicity coefficient is greater than 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as summation action.
(4) result and analysis:
The composite co-toxicity test of table 1 bixafen and carbendazim to the cucumber downy mildew germ
The composite co-toxicity test of table 2 bixafen and carbendazim to the cucumber downy mildew germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Carbendazim (B) | 0∶1 | 45.21 | 45.21 | / | / |
| A∶B | 3∶19 | 76.57 | 52.69 | 145.33 | Synergy |
| A∶B | 5∶17 | 88.73 | 57.67 | 153.87 | Synergy |
| A∶B | 7∶15 | 99.83 | 62.65 | 159.36 | Synergy |
| A∶B | 9∶13 | 98.75 | 67.63 | 146.02 | Synergy |
| A∶B | 11∶11 | 100.25 | 72.61 | 138.07 | Synergy |
Measuring the result according to above indoor virulence test shows; Bixafen and carbendazim all have synergistic effect preferably by the percentage composition of experiment; Wherein with bixafen: carbendazim is that 7: 15 o'clock co-toxicity coefficient is up to 159.36, thinks that bixafen and carbendazim percentage composition are that 7: 15 o'clock synergistic effects are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 15 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
The composite Toxicity Determination of biologicall test instance 2:bixafen and thiophanate-methyl to the powdery mildew of cucumber germ.
The former medicine of 95% thiophanate-methyl, Jiangsu lark farmingization Co., Ltd.
The powdery mildew of cucumber germ, separation and purification gained from the powdery mildew of cucumber incidence of leaf of Shouguang, Shandong cucumber booth is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent bixafen and thiophanate-methyl are carried out toxicity test, EC
50Value is followed successively by 3.06mg/l and 6.71mg/l.
Combination setting is mixed:
25%bixafen thiophanate-methyl percentage composition was respectively 5: 20,7: 18,9: 16,11: 14,13: 12, i.e. 5%+20%, 7%+18%, 9%+16%, 11%+14%, 13%+12% totally 5 combinations that are mixed.
Test method is with biologicall test embodiment 1
Experimental result and analysis:
The composite virulence experiment of table 3 bixafen and thiophanate-methyl to the powdery mildew of cucumber germ
The composite co-toxicity test of table 4 bixafen and thiophanate-methyl to the powdery mildew of cucumber germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Thiophanate-methyl (B) | 0∶1 | 45.60 | 45.60 | / | |
| A∶B | 5∶20 | 82.56 | 56.48 | 146.16 | Synergy |
| A∶B | 7∶18 | 96.77 | 60.83 | 159.07 | Synergy |
| A∶B | 9∶16 | 100.20 | 65.19 | 153.71 | Synergy |
| A∶B | 11∶14 | 99.69 | 69.54 | 143.36 | Synergy |
| A∶B | 13∶12 | 103.48 | 73.89 | 140.05 | Synergy |
Measuring the result according to above indoor virulence test shows; The used bixafen and the percentage composition of thiophanate-methyl all have synergistic effect in the experiment; Wherein the co-toxicity coefficient when the percentage composition of bixafen and thiophanate-methyl is 7: 18 is 159.07 to the maximum, thinks that promptly 7: 18 o'clock synergistic effects of percentage composition of bixafen and thiophanate-methyl are best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 18 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
The composite Toxicity Determination of biologicall test instance 3:bixafen and probenazole to citrus common green mold.
The citrus common green mold germ, separation and purification gained from the morbidity oranges and tangerines in oranges and tangerines garden, Jiangxi is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent bixafen and probenazole are carried out toxicity test, EC
50Value is followed successively by 2.77mg/l and 6.32mg/l.
Combination setting is mixed:
The percentage composition of 21%bixafen probenazole was respectively 3: 18,5: 16,7: 14,9: 12,11: 10, i.e. 3%+18%, 5%+16%, 7%+14%, 9%+12%, 11%+10% totally 5 combinations that are mixed.
Test method is similar with biologicall test embodiment 1
Result and analysis:
The composite co-toxicity of table 5 bixafen and probenazole to the citrus common green mold germ
The composite co-toxicity of table 6 bixafen and probenazole to the citrus common green mold germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Probenazole (B) | 0∶1 | 43.83 | 43.83 | / | / |
| A∶B | 3∶18 | 71.15 | 51.85 | 137.22 | Synergy |
| A∶B | 5∶16 | 83.90 | 57.20 | 146.67 | Synergy |
| A∶B | 7∶14 | 95.99 | 62.55 | 153.45 | Synergy |
| A∶B | 9∶12 | 95.82 | 67.90 | 141.11 | Synergy |
| A∶B | 11∶10 | 94.53 | 73.25 | 129.05 | Synergy |
Mensuration result according to the test of bactericide co-toxicity; When bixafen and probenazole percentage composition are above experiment ratio; Result of the test shows all has synergistic effect; And the co-toxicity coefficient when ratio is 7: 14 is 153.45 to the maximum, and we can think that the percentage composition of bixafen and probenazole is at 7: 14 o'clock, and synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 7: 14 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
The composite Toxicity Determination of biologicall test instance 4:bixafen and benomyl to citrus scab.
The citrus scab germ, the separation and purification gained on the sick leaf of typical satsuma mandarin shot hole of falling ill from the Taihe County, Jiangxi Province is cultivated in 4 ℃ of refrigerators on the PDA inclined-plane and is preserved.
Single agent bixafen and benomyl are carried out toxicity test, EC
50Value is followed successively by 3.44mg/l and 8.03mg/l.
Combination setting is mixed:
The percentage composition of 23%bixafen benomyl was respectively 3: 20,5: 18,7: 16,9: 14,11: 12, i.e. 3%+20%, 5%+18%, 7%+16%, 9%+14%, 11%+12% totally 5 combinations that are mixed.
Test method is similar with biologicall test embodiment 1
Result and analysis:
The composite co-toxicity of table 7 bixafen and benomyl to the citrus scab germ
| The pharmacy quality ratio | Virulence regression equation | Correlation coefficient (r) | EC 50(mg/L) |
| bixafen(A) | Y＝1.2371X+4.3362 | 0.9533 | 3.44 |
| Benomyl (B) | Y＝1.3647X+3.7653 | 0.9624 | 8.03 |
| A: B is 3: 20 | Y＝1.3306X+4.0625 | 0.9701 | 5.06 |
| A: B is 5: 18 | Y＝1.2948X+4.2119 | 0.9488 | 4.06 |
| A: B is 7: 16 | Y＝1.4009X+4.1881 | 0.9567 | 3.80 |
| A: B is 9: 14 | Y＝1.3647X+4.2317 | 0.9391 | 3.66 |
| A: B is 11: 12 | Y＝1.2958X+4.2934 | 0.9405 | 3.51 |
The composite co-toxicity of table 8 bixafen and benomyl to the citrus scab germ
| Test medicine | Mass ratio | The actual measurement poison refers to | Theoretical poison refers to | Co-toxicity coefficient | Conclusion |
| Bixafen(A) | 1∶0 | 100.00 | 100.00 | / | / |
| Benomyl (B) | 0∶1 | 42.84 | 42.84 | / | / |
| A∶B | 3∶20 | 67.92 | 50.30 | 135.05 | Synergy |
| A∶B | 5∶18 | 84.72 | 55.27 | 153.29 | Synergy |
| A∶B | 7∶16 | 90.58 | 60.24 | 150.37 | Synergy |
| A∶B | 9∶14 | 94.11 | 65.21 | 144.33 | Synergy |
| A∶B | 11∶12 | 98.01 | 70.18 | 139.66 | Synergy |
Mensuration result according to the test of bactericide co-toxicity; When bixafen and benomyl percentage composition are above experiment ratio; Result of the test shows all has synergistic effect; And the co-toxicity coefficient when ratio is 5: 18 is 153.29 to the maximum, and we can think that the percentage composition of bixafen and benomyl is at 5: 18 o'clock, and synergistic effect is best.Suggestion is carried out further field control effectiveness test to the mixture preparation of 5: 18 left and right sides scopes of suitable proportion, to estimate its field practical application effect.
Field trial 1: to the preventive effect of citrus scab
(1) investigation method
Experimental field be located at 15 years living satsuma mandarins garden, wingceltis village, wingceltis villages and small towns, Jiande City and carry out, 4 repetitions are established in every processing, every repetition 10 strains, and randomized arrangement, guard row is established in the centre.Every fixing investigation 4 strains, 10 at every strain investigation spring tip, 60 blades, 30 of young fruits of repeating.April 20, Mays 10 daily adopt knapsack sprayer to spray tree crown, and each sprays medicine once, and the soup consumption is 1201/667m
2
Come classification according to the citrus scab grade scale, grade scale does
0 grade: blade does not have scab, young fruit does not have scab;
1 grade: blade 1～5 scab, young fruit have a small amount of scab;
2 grades: 6～15 scabs of blade, young fruit 1/3 fruit face have scab;
3 grades: 16 above scabs of blade, but blade is non-warping, young fruit 1/2 fruit face has scab;
4 grades: the leaf malformation distortion, full fruit has scab;
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 9 is respectively organized the test medicine processing and is prevented and treated the test effect of citrus scab
Interpretation of result:
Can know by table 11; The effect that 5: 18 the combination that is mixed of 22%bixafen benomyl prevents and treats citrus scab is more remarkable; Behind the medicine 10 days for the first time, 10 days and 20 days preventive effects 85.48%, 87.12% and 88.99% the highest behind the medicine for the second time, 20 days preventive effects the highest are 79.42% behind the single agent 10%bixafen EC medicine second time; 20 days preventive effect is up to 79.95% behind the 50% benomyl WP medicine second time; The effect of complex preparation significantly is superior to two kinds of single agent, has not only significantly improved the effect for leaf spoting bacteria, and has further prolonged its lasting period.
Field trial 2: to the preventive effect of powdery mildew of cucumber
(1) investigation method
Experimental field be located at Shouguang, Shandong, publish the medium cucumber field of course of disease degree for experimental field, soil fertility is medium, and irrigation and drainage are convenient.Adopt the bilinear sampling method, 5 points are got in every sub-district, look into 5 caves at every, and 2 blades in the fixing middle part of every strain fix a point to check during investigation.
Come classification according to the powdery mildew of cucumber grade scale, write down total strain number, diseased plant number, degree of being in a bad way, calculate diseased plant rate, disease index and preventive effect.The powdery mildew of cucumber grade scale is:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Test is investigated 4 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(2) control time and number of times
Test is investigated 3 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 10 days and preventive effect investigation in 20 days behind 2 medicines.
(3) drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
(4) poisoning investigation method
Whether continuous 7d range estimation medicament has poisoning to crop after the dispenser.
(5) experimental result and analysis
Table 10 is respectively organized the test medicine processing and is prevented and treated the test effect of powdery mildew of cucumber
Interpretation of result:
Can know that by table 10 effect that 7: 18 the combination that is mixed of 25%bixafen thiophanate-methyl prevents and treats powdery mildew of cucumber is very remarkable.Under same 10 milliliters every mu consumption, the effect of complex preparation significantly is superior to two kinds of single agent, and its quick-acting and lasting effect are all relatively good, and is the most outstanding with lasting effect especially.Complex preparation significantly is superior to the effect under the low concentration consumption in control efficiency under the consumption of high concentration.20 days preventive effects reach 90.98% after 10 days best control efficiency behind the 1st, 2 medicine reaches 85.26% and 89.66%, the 2 medicine respectively.And single agent 10%bixafen EC; 10 days the highest preventive effect is 83.27% behind the 2nd medicine; 20 days preventive effects are 83.79% behind the 2nd medicine of single agent 70% thiophanate-methyl WP; The effect of complex preparation significantly is superior to two kinds of single agent, has not only significantly improved the effect for the powdery mildew of cucumber germ, and has further prolonged its lasting period.
Comprehensive above experiment is said; With new type bactericide bixafen and part benzimidazole germicide benomyl, carbendazim, thiophanate-methyl, probenazole, furidazol is that active ingredient is carried out two yuan of bactericidal composition preparations that are mixed the diseases such as leaf spot, powdery mildew, rust, downy mildew, eqpidemic disease, anthracnose and rice blast of various crop is all had the better prevention effect; Can effectively suppress further developing of disease; Simultaneously to crop safety; Suggestion is promoted the use of, and in use should be used alternatingly with other different mechanisms medicines, to delay the pesticide resistance of plant.
Claims (7)
1. pesticide sterilizing composite, it is characterized in that: this bactericidal composition is that (molecular formula is C with A novel pyrazole series bactericidal agent bixafen
18H
12Cl
2F
3N
3O) with B part benzimidazoles compound benomyl, carbendazim, thiophanate-methyl, probenazole, furidazol in one of any be the preparation of two yuan of composite bactericidal compositions of active ingredient, all the other components are usual auxiliaries in the agricultural chemicals,
The A novel pyrazoles kills letter agent bixafen.
2. bactericidal composition according to claim 1 is characterized in that: the percentage by weight of effective constituents A and B is 80: 1～1: 90 in this bactericidal composition.
3. bactericidal composition according to claim 1 is characterized in that: the percentage by weight of effective constituents A and B is 50: 3～5: 65 in this bactericidal composition.
4. bactericidal composition according to claim 1 is characterized in that: to account for the percentage of gross weight in the preparation be 1%～90% for effective constituents A and B total content in this bactericidal composition.
5. bactericidal composition according to claim 4 is characterized in that: effective constituents A and the B gross weight percentage composition in preparation is preferably 8%～75%.
6. bactericidal composition according to claim 1 is characterized in that: the preparation formulation that bactericidal composition of the present invention can be mixed with is wetting powder, suspending agent, soluble liquid, aqueous emulsion and water dispersible granules.
7. according to the described bactericidal composition of claim 1 to 7, it is characterized in that: bactericidal composition of the present invention be mainly used in control grain, cotton, oil, really, the fungal disease of the crops of vegetables, flowers.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105685077A (en) * | 2015-12-24 | 2016-06-22 | 南京华洲药业有限公司 | Sterilization composition containing bixafen and thiophanate-methyl and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070060579A1 (en) * | 2003-10-10 | 2007-03-15 | Ulrike Wachendorff-Neumann | Synergistic fungicidal active substance combinations |
| CN101969781A (en) * | 2007-10-23 | 2011-02-09 | 杜邦公司 | Fungicidal compounds and mixtures |
| CN102014628A (en) * | 2008-02-05 | 2011-04-13 | 巴斯夫欧洲公司 | Plant health composition |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070060579A1 (en) * | 2003-10-10 | 2007-03-15 | Ulrike Wachendorff-Neumann | Synergistic fungicidal active substance combinations |
| CN101969781A (en) * | 2007-10-23 | 2011-02-09 | 杜邦公司 | Fungicidal compounds and mixtures |
| CN102014628A (en) * | 2008-02-05 | 2011-04-13 | 巴斯夫欧洲公司 | Plant health composition |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105685077A (en) * | 2015-12-24 | 2016-06-22 | 南京华洲药业有限公司 | Sterilization composition containing bixafen and thiophanate-methyl and application thereof |
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Application publication date: 20120111 |