CN103444741B - Penconazole and zhongshengmycin-containing sterilization composition and application thereof - Google Patents
Penconazole and zhongshengmycin-containing sterilization composition and application thereof Download PDFInfo
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- CN103444741B CN103444741B CN201310407033.3A CN201310407033A CN103444741B CN 103444741 B CN103444741 B CN 103444741B CN 201310407033 A CN201310407033 A CN 201310407033A CN 103444741 B CN103444741 B CN 103444741B
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- penconazole
- zhongshengmycin
- bactericidal composition
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- 239000002195 soluble material Substances 0.000 description 1
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- NLVFBUXFDBBNBW-PBSUHMDJSA-N tobramycin Chemical compound N[C@@H]1C[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N NLVFBUXFDBBNBW-PBSUHMDJSA-N 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of pesticide compounding techniques, particularly relates to a penconazole and zhongshengmycin-containing sterilization composition, and also relates to an application thereof. The penconazole and zhongshengmycin-containing sterilization composition comprises effective ingredients of penconazole and zhongshengmycin with a weight part ratio of (90:1)-(1:90), and the penconazole and zhongshengmycin-containing sterilization composition is prepared into a wettable powder, a suspending agent, an emulsion in water, a water dispersible granule, emulsifiable concentrate and the like after being added with assistants and an excipient, and is used for controlling fungal diseases caused by germs such as erysiphe, uromyces, mortierella, septoria and venturia and bacterial diseases caused by germs such as Gram-positive bacteria, Gram-negative bacteria, mycobacteria and saccharomycetes. The penconazole and zhongshengmycin-containing sterilization composition has a relatively good synergism within a certain range, is superior to a single preparation on the aspect of control effects, is relatively durable in pesticide effect, has a special effect on controlling powdery mildew, rust diseases, scab, leaf spot diseases, gray mold, white rot, soft rot, streak diseases, rice blast, ring spot, gibberellic diseases and bacterial diseases, is relatively low in residue, is relatively easily decomposed after a lasting period, is relatively environment-friendly, and is beneficial to the increase of yield and income in an agricultural district.
Description
Technical field
The present invention relates to technical field of pesticide combination, be specifically related to a kind of bactericidal composition containing penconazole and Zhongshengmycin, also relate to the application of this bactericidal composition.
Background technology
Penconazole, chemical name: 1-[2-(2,4-dichlorophenyl) amyl group]-1H-1,2,4-triazole, belong to triazole bactericide, for ergosterol demethylation inhibitors, to disease caused by the Erysiphe on cereal crop, vegetables, fruit tree, peanut, Rust, the mould Pseudomonas of spore, Septoria, Venturia germ, there is good control efficiency, but when applying separately, control spectrum is narrower, and when using for a long time, germ easily develops immunity to drugs, and in addition, it is higher to be used alone this reagent cost.
The chemical structural formula of penconazole is:
Zhongshengmycin is a kind of novel agricultural antibiotic that biological control research institute of the Chinese Academy of Agricultural Sciences succeeds in developing, and is produced by the mutation of Strepiomyces lavendulae Hainan, belongs to N-glucosides class alkali water-soluble material.The processing formulation of this bacterium is the wider protective fungicide of a kind of fungicidal spectrum, and have and tag and osmosis, its mechanism of action is: synthesized by anti-bacteria bacterium protein and make microorganism dead; By making mold fungi deformation of filament, suppressing spore germination or directly kill the modes such as spore to act on filamentous fungi; Can the generation of the precursor substance of protective plant protecting agent and lignin in stimulating plant body, thus improve the resistance against diseases of plant, be beneficial to crop yield and increase income.
Zhongshengmycin is N-sugar tobramycin antibiotic, and antimicrobial spectrum is very wide, can resist Gram-positive, negative bacteria well, mycobacterium, the multiple diseases that saccharomycete and filamentous fungi cause.To Bacteria erwinia, angular leaf spot fungus, leaf spot bacteria, Pyricularia oryzae, Target spot pathogen, gibberellic hypha etc., all there is obvious antibacterial activity.
Penconazole and Zhongshengmycin all have higher bactericidal activity, if but be used alone for a long time, all easily make plant germ develop immunity to drugs, then produce the problems such as dosage increasing, preventive effect reduction, lasting period shortening.Meanwhile, the Reusability of agricultural chemicals also can cause the degradation rate of agricultural chemicals in soil to decline, and is unfavorable for environment sustainable development very much.The active ingredient of the different mechanism of action being carried out reasonably composite, is delay disease to develop immunity to drugs very effective common method, and can according to the effect in production application, judge this composite be synergistic effect or antagonism.The formula that two kinds of agriculture chemical compounding compatibilities are good, can significantly improve preventive effect, greatly reduce the dosage of agricultural chemicals, can also expand fungicidal spectrum, improves germicidal efficiency.
In the present composition, two kinds of active component mechanism of action points are different, the present inventor is through lot of experiments recipe determination, propose a kind of Compositional type bactericidal composition of the present invention, namely with two yuan of combination sterilization compositions that penconazole and Zhongshengmycin carry out for active ingredient.Because these two kinds of active component mechanism of action points are different, by penconazole and Zhongshengmycin composite in certain proportion, there is good synergy synergy, substantially increase germicidal efficiency, and well can delay the generation of Disease Resistance, expand fungicidal spectrum, reduce drug cost, improve actual preventive effect, can be used for preventing and treating agriculture vegetables, fruit tree, the multiple fungal disease of flowers and other crops and various bacteria disease, especially rice blast and banded sclerotial blight preventive effect are given prominence to.And composite about penconazole and Zhongshengmycin, there is not yet relevant report at home and abroad at present.
Summary of the invention
One has synergistic function, use cost is low to the object of the invention is proposition, the bactericidal composition containing penconazole and Zhongshengmycin that preventive effect is good.
The present invention is achieved through the following technical solutions:
Contain a bactericidal composition for penconazole and Zhongshengmycin, this bactericidal composition comprises active ingredient penconazole and Zhongshengmycin.
Its formulation of described bactericidal composition is any one in wetting powder, suspending agent, aqueous emulsion, water dispersible granules and missible oil.
When bactericidal composition is wetting powder, the percentage by weight of each component is: penconazole 1% ~ 90%, Zhongshengmycin 1% ~ 90%, dispersant 1% ~ 20%, wetting agent 1% ~ 10%, filler 1% ~ 70%, by above-mentioned weight proportion by penconazole and Zhongshengmycin and the mixing of dispersant, wetting agent and filler, uniform stirring in stirred tank, mix again after airslide disintegrating mill is pulverized, obtain wetting powder bactericidal composition;
When bactericidal composition is suspending agent, the percentage by weight of each component is: penconazole 1% ~ 60%, Zhongshengmycin 1% ~ 60%, dispersant 5% ~ 20%, antifreezing agent 1% ~ 5%, thickener 0.1% ~ 2%, defoamer 0.1% ~ 0.8%, surplus is water, the concrete production stage of this suspending agent is: first mixed by above-mentioned proportioning by auxiliary agent, mix through high speed shear, add penconazole and Zhongshengmycin, abrading-ball 2 ~ 3 hours in ball crusher, make a diameter all at below 5mm, obtain the bactericidal composition of aqueous suspension formulations;
When bactericidal composition is aqueous emulsion, the percentage by weight of each component is: penconazole 1% ~ 60%, Zhongshengmycin 1% ~ 60%, emulsifier 3% ~ 30%, solvent 5% ~ 15%, stabilizing agent 0.2% ~ 15%, antifreezing agent 0.1% ~ 5%, defoamer 0.1% ~ 8%, thickener 0.1% ~ 2%, surplus are water; The concrete production stage of this aqueous emulsion is: penconazole and Zhongshengmycin, solvent and emulsifier are mixed, and makes it to be dissolved into uniform oil phase; Water, antifreezing agent, stabilizing agent, thickener and defoamer are mixed uniformly aqueous phase; While high-speed stirred, oil phase is added aqueous phase in a kettle., slowly add water until reach phase inversion point, open clipper and carry out high speed shear, shear 20 minutes to 1 hour, form the aqueous emulsion of oil-in-water type, obtain the bactericidal composition of aqueous emulsion;
When bactericidal composition is water dispersible granules, the mass percent of component is: penconazole 1% ~ 90%, Zhongshengmycin 1% ~ 90%, dispersant 3% ~ 20%, wetting agent 3% ~ 10%, disintegrant 1% ~ 5%, filler 1% ~ 70%, the concrete production stage of this aqueous emulsion is: penconazole, Zhongshengmycin and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediating, then add in fluidized-bed granulation dryer and carry out granulation, drying, screening, obtain the bactericidal composition of water dispersant;
When bactericidal composition is missible oil, the mass percent of component is: penconazole 1% ~ 60%, Zhongshengmycin 1% ~ 60%, emulsifier 1% ~ 15%, surplus is solvent, the concrete production stage of this missible oil is: first penconazole and Zhongshengmycin are added in solvent according to above-mentioned ratio and dissolve completely, then add the oily liquids that emulsifier for mixing evenly becomes transparent and homogeneous afterwards, obtain the bactericidal composition of cream preparation;
Wherein, the mass ratio of penconazole and Zhongshengmycin is any one ratio in 3:1,1.5:1,1:1,1:1.5,1:3.
Described dispersant is: at least one in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, fatty acid glyceride APEO;
Described wetting agent is: at least one in lauryl sodium sulfate, calcium dodecyl benzene sulfonate, Nekal BX, wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene tri-styryl phosphenylic acid salt, spaonin powder, silkworm excrement, soapberry powder;
Described disintegrant is: at least one in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, sodium bicarbonate;
Described emulsifier is: any one or more and calcium dodecyl benzene sulfonate in aliphatic acid polyethenoxy ether, alkylphenol polyoxyethylene sulfosuccinate, styrylphenol polyoxyethylene ether, polyoxyethylene nonylphenol ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether and the mixed solvent formed with arbitrary proportion;
Described solvent is any one or more mixed solvents formed with arbitrary proportion in dimethylbenzene, biodiesel, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), aqueous solvent; Described filler is: at least one in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, precipitated calcium carbonate;
Described thickener is: at least one in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, polyvinyl alcohol;
Described antifreezing agent is: at least one in ethylene glycol, propane diols, glycerine, polyethylene glycol;
Described stabilizing agent is: at least one in sodium citrate, resorcinol;
Described defoamer is: silicone oil, silicone compound, C
10-20at least one in saturated fat acid compounds, C8-10 aliphatic alcohols compound.
The application of bactericidal composition of the present invention in control rice blast and rice sheath blight disease is also the scope that will protect.
Beneficial effect of the present invention:
(1) present composition effective active composition penconazole and Zhongshengmycin belong to the bactericide of two kinds of different mechanisms of action, and both can not produce conflict at mutual mixture;
(2) present composition has good synergistic effect within the specific limits, and preventive effect is higher than single dose, and drug effect is more lasting;
(3) delay drug-fast generation, decreased agricultural chemicals Reusability number of times, reduced Pesticide use amount, reduce the use cost of peasant;
(4) present composition has special efficacy to rice blast and rice sheath blight disease etc.
(5) residual quantity due to this bactericidal composition is lower, decomposes after the lasting period than being easier to, more friendly to environment, has larger meaning to the increasing both production and income of agricultural region.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
The method that the present invention adopts indoor bioassay and field trial to combine.If no special instructions, the ratio (comprising percentage) below mentioned is all mass ratio.It should be pointed out that all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
The implementation of Formulation Example
Embodiment 1:20% penconazole Zhongshengmycin wetting powder
15% penconazole, 5% Zhongshengmycin, 5% polycarboxylate, 5% lauryl sodium sulfate and 70% kaolin are mixed, uniform stirring in stirred tank, mix again after airslide disintegrating mill is pulverized, obtained 50% penconazole Zhongshengmycin wetting powder.
Embodiment 2:20% penconazole Zhongshengmycin wetting powder
10% penconazole, 10% Zhongshengmycin, 5% lignosulfonates, 3% wetting and penetrating agent F and 72% kaolin are mixed, uniform stirring in stirred tank, mix again after airslide disintegrating mill is pulverized, obtained 20% penconazole Zhongshengmycin wetting powder.
Embodiment 3:20% penconazole Zhongshengmycin suspending agent
5% lignosulfonates, 3% propane diols, 1% xanthans, 0.6% silicone oil and 70.4% water are mixed, after high speed shear mixes, add 10% penconazole and 10% Zhongshengmycin, abrading-ball 2 ~ 3 hours in ball crusher, make a diameter all at below 5mm, obtained 20% penconazole Zhongshengmycin suspending agent.
Embodiment 4:25% penconazole Zhongshengmycin suspending agent
6% alkylphenol polyoxyethylene, 4% ethylene glycol, 1% xanthans, 0.5% silicone oil and 63.5% water are mixed, after high speed shear mixes, add 20% penconazole and 5% Zhongshengmycin, abrading-ball 2 ~ 3 hours in ball crusher, make a diameter all at below 5mm, obtained 25% penconazole Zhongshengmycin suspending agent.
Embodiment 5:20% penconazole Zhongshengmycin aqueous emulsion
By 15% penconazole, 5% Zhongshengmycin, 5% castor oil polyoxyethylene ether, 25% dimethylbenzene mixing, make to be dissolved into uniform oil phase; 2% propane diols, 1% silicone oil, 3% sodium citrate and 0.5% xanthans and 43.5% water are mixed uniformly aqueous phase; While high-speed stirred, oil phase is added aqueous phase in a kettle., slowly add water until reach phase inversion point, open clipper and carry out high speed shear, and add remaining water, shear about half an hour, form the aqueous emulsion of oil-in-water type, obtained 20% penconazole Zhongshengmycin aqueous emulsion.
Embodiment 6:40% penconazole Zhongshengmycin water dispersible granules
30% penconazole, 10% Zhongshengmycin, 8% lignosulfonates, 5% lauryl sodium sulfate, 3% ammonium sulfate and 44% diatomite are mixed, pulverize with micro jet, through mediating, then add in fluidized-bed granulation dryer and carry out through sample analysis after granulation, drying, screening, obtained 40% penconazole Zhongshengmycin water dispersible granules.
Embodiment 7:20% penconazole Zhongshengmycin water dispersible granules
Phonetic to 15% penconazole, 5% Zhongshengmycin, 5% alkylphenol-polyethenoxy, 3% lauryl sodium sulfate, 10% starch and 62% bentonite are mixed, pulverize with micro jet, through mediating, then add in fluidized-bed granulation dryer and carry out through sample analysis after granulation, drying, screening, obtained 20% penconazole Zhongshengmycin water dispersible granules.
Embodiment 8:20% penconazole Zhongshengmycin missible oil
15% penconazole, 5% Zhongshengmycin are joined in 69% dimethylbenzene, after dissolving completely, add the oily liquids becoming transparent and homogeneous after 6.0g castor oil polyoxyethylene ether stirs with 5.0g calcium dodecyl benzene sulfonate, obtained 20% penconazole Zhongshengmycin missible oil.
Penconazole and Zhongshengmycin composite to target bacterium Toxicity Determination
Experiment adopts rice blast fungus to be tested object.
(1) experimental pharmacy:
The former medicine of 95% penconazole, Hangzhou Yu Long Co., Ltd, 95% zhongshengmycin bulk drug, Shenyang Sciencreat Chemicals Co., Ltd..
Penconazole and Zhongshengmycin proper amount of acetone are dissolved, and are made into the mother liquor of 10000 μ g/ml, Cord blood at 4 DEG C, stand-by, test front sterile water and are diluted to a series of concentration.
Mixture combination is arranged:
Test 15% penconazole Zhongshengmycin wetting powder mass ratio and be respectively 8:1,4:1,1:1,1:4,1:8, totally 5 mixture combinations.
(2) experimental subjects
Rice Pyricularia grisea, gathers separation and purification gained rice pathogenesis blade from Guizhou, PDA inclined-plane is cultivated in 4 DEG C of refrigerators and preserves.
For examination medium:
PDA medium: potato 200g, glucose 20g, agar 20g, water 1000mL;
AEA medium: dusty yeast 5g/L, glycerine 20mL/L, MgSO4 0.25g/L, NaNO3 6g/L, KCl0.5g/L, KH2PO4 1.5g/L, agar powder 20g/L, deionized water 1L.
(3) experimental technique
Single dose is to the toxicity test of rice Pyricularia grisea:
First prepare spore suspension, have to cultivation on the product spore medium of Pyricularia oryzae and add appropriate amounts of sterilized water, gently brush with writing brush, basis of microscopic observation, under low power lens about 100 spores in every visual field.
Secondly from low concentration to high concentration, draw 60 μ L liquids successively with liquid-transfering gun to add respectively in small test tube, then absorption spore suspension 60 μ L, make liquid and spore suspension mixed in equal amounts even.Drawing 30 μ L mixed liquors with liquid-transfering gun drips on the slide with fine jade film-making, and frame is put in the large culture dish with shoaling layer, adds a cover and is placed in 28 DEG C, and the mold incubator of RH80% cultivates 20 ~ 24h.Often process repetition 4 times, compare with the spore suspension of not adding medicine.
When blank spore germination rate reaches more than 90%, check each process spore germination situation.
Often process each repetition random observation 3 visuals field, often process about investigation 200 spores, record spore germination number and spore sum respectively with counter.
With the log concentration value of reagent agent for independent variable, with spore germination relative inhibition probit value for dependent variable, carry out data analysis statistical by DPS data handling system, obtain virulence regression equation, EC50 value and correlation coefficient.
Spore tooth length of tube is greater than spore radius and is considered as sprouting.
Spore germination rate=(spore germination number/investigation spore sum) × 100
Spore germination rate=(process spore germination rate/blank spore germination rate) × 100 are corrected in process
Spore germination relative inhibition=(spore germination rate/blank spore germination rate is corrected in process) × 100
On the basis of preliminary experiment, respectively toxicity test is carried out to single dose penconazole and Zhongshengmycin in order to upper method, the two EC
50value is respectively 0.6223mg/L and 2.1893mg/L.
20% penconazole Zhongshengmycin wetting powder mass ratio is respectively 3:1,1.5:1,1:1,1:1.5,1:3, i.e. 15%+5%, 12%+8%, 10%+10%, 8%+12%, 5%+15% totally 5 mixture combinations.
(4) data statistics and analysis
Carry out the toxicity test of mixture according to mixed ratio by single dose toxicity test method.
According to the EC of the abundant method of Sun Yun by each process of mensuration
50value is converted into actual mixed toxicity index (ATI); According to the proportioning of mixture, obtain theoretical mixed toxicity index (TTI), by the co-toxicity coefficient (CTC) of following formulae discovery mixture.
Single dose toxicity index=(standard agent EC
50/ reagent agent EC
50) × 100
ATI=(standard agent EC
50/ reagent agent (using with) EC
50) × 100
The content (%) of toxicity index × B in using with of content (the %)+B of toxicity index × A in using with of TII=A
CTC＝(ATI/TTI)×100
If co-toxicity coefficient large 120, shows there is synergistic effect; If be starkly lower than 100 (less than 80), be indicated as antagonism; Between 100 ~ 120, be indicated as summation action.
(5) toxicity test result and analysis
Experimental example 2: experimental subjects is still rice Pyricularia grisea, method is roughly with above experiment.
On the basis of preliminary experiment, respectively toxicity test is carried out to single dose penconazole and Zhongshengmycin in order to upper method, the two EC
50value is respectively 0.6223mg/L and 2.1893mg/L.
Test and be respectively 3:1,1.5:1,1:1,1:1.5,1:3, a totally 5 mixture combination with 20% penconazole Zhongshengmycin wetting powder mass ratio.
Toxicity test result and analysis
Table 1 penconazole and the composite co-toxicity to rice blast fungus of Zhongshengmycin are tested
| Medicament and proportioning | Virulence regression equation | Correlation coefficient (r) | EC 50Value (mg/L) | Co-toxicity coefficient |
| Penconazole (A) | y＝1.2758x+5.2628 | 0.9766 | 0.6223 | / |
| Zhongshengmycin (B) | y＝1.1469x+4.6097 | 0.9959 | 2.1893 | / |
| A:B is 3:1 | y＝1.3674x+5.3083 | 0.9959 | 0.5950 | 127.38 |
| A:B is 1.5:1 | y＝1.3892x+5.4262 | 0.9764 | 0.4934 | 176.72 |
| A:B is 1:1 | y＝1.3515x+5.3003 | 0.9711 | 0.5995 | 161.66 |
| A:B is 1:1.5 | y＝1.3874x+5.0925 | 0.9470 | 0.8577 | 127.17 |
| A:B is 1:3 | y＝1.4645x+4.9702 | 0.9773 | 1.0480 | 128.20 |
Visible according to above joint toxicity measuring result, co-toxicity coefficient when 20% penconazole Zhongshengmycin wetting powder is 3:1,1.5:1,1:1,1:1.5 and 1:3 percentage composition is all greater than 120, all show as synergistic effect, wherein when penconazole and Zhongshengmycin mass ratio are 1.5:1, co-toxicity coefficient is the highest, and synergistic effect is best.Suggestion carries out further field control effectiveness test to the mixture preparation of suitable proportion about 1.5:1 scope, to evaluate its field practical application effect.
Penconazole, Zhongshengmycin and composite control rice blast effect experiment thereof
(1) test method
Test dispenser twice altogether, check plot spray equivalent clear water.When making up a prescription, after first medicament fully being dissolved with a small amount of water, then add suitable quantity of water and carry out all-out atomizing process, even spraying, spray emphatically rice strain middle and lower part.All adopt power driven sprayer during spray medicine, operating pressure is 2.0 ~ 2.3kg/cm
2, shower nozzle aperture 1.2mm.Community spouting liquid presses 675L/10000m
2use, liquid is sprayed application to uniformly the positive and negative two sides of crop leaf.
(2) investigation method
Experimental field be located at rice in Hunan field, select the field of growing way more consistent leaf seasonal febrile diseases their early stage to be experimental field, middle fertility, well-managed.
Every community gets 4 points at random, five strains whole blade during investigation, and a situation arises for statistics disease.
Every sheet leaf accounts for the percentage classification of leaf area by scab, records total number of sheets, the sick number of sheets at different levels, calculates the blade incidence of disease, disease index, and contrast disease and refers to compare, calculating relative control effect.
Severity Scaling standard (in units of blade)
Rice leaf seasonal febrile diseases leaf rate Assessment for classification standard:
0 grade: without scab on blade;
1 grade: scab is syringe needle shape size foxiness;
2 grades: slightly large brown point;
3 grades: circular to oval grey scab, edge brown, lesion diameter about 1 ~ 2 millimeter;
4 grades: typical spindle scab, long 1 ~ 2 centimetre, be usually confined between 2 veins, area of causing harm is no more than 2% of blade area;
5 grades: typical spindle scab, area of causing harm is no more than 10% of leaf area;
6 grades: typical spindle scab, area 10.1 ~ 25% of causing harm;
7 grades: typical spindle scab, area 25.1 ~ 50% of causing harm;
8 grades: typical spindle scab, area 50.1 ~ 75% of causing harm;
9 grades: full leaf is withered.
Panicle blast disease tassel yield Assessment for classification standard:
0 grade: anosis;
1 grade: disease tassel yield is lower than 1 ~ 5%;
3 grades: disease tassel yield is lower than 5.1 ~ 10%;
5 grades: disease tassel yield is lower than 10.1 ~ 25%;
7 grades: disease tassel yield is lower than 25.1 ~ 50%;
9 grades: disease tassel yield is lower than 50.1 ~ 100%.
Leaf pest control time: in the rice tillering intermediary and later stages, leaf blast in rice enters their early stage investigation, investigation disease index before medicine, respectively investigates once, within after secondary medicine 15 days, observes its lasting effect, investigate 4 times altogether for after first and second medicine 7 days.
Neck pest control time: in paddy stage of yellow ripeness, investigate after neck pest stable disease, after one week, check once.
Test is investigated 4 times altogether, i.e. disease index investigation before medicine, preventive effect investigation in 7 days after the 1st medicine, 7 days and preventive effect investigation in 15 days after 2 medicines.
(3) poisoning investigation
Whether after dispenser, continuous 7d estimates medicament has poisoning to crop.
(4) drug effect computational methods
Sick leaf rate (%)=sick number of sheets/investigate the total number of sheets × 100
Disease index=∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=(1-(before blank district medicine after disease index × treatment region medicine disease index)/(after blank district medicine before disease index × treatment region medicine disease index)) × 100
(5) field control effectiveness test result
The experiment effect of rice blast prevented and treated by table 2 respectively group experimental pharmacy process
Interpretation of result:
As shown in Table 2, the Be very effective of the mixture combination control rice blast of 20% penconazole Zhongshengmycin WP (1.5:1).After first time medicine 7 days, after second time medicine, 7 days and 15 days preventive effects the highest were respectively 76.93%, 81.38% and 84.63%.The effect of 25% penconazole EW single dose control rice blast is better than 6% Zhongshengmycin WP single dose, and all within 15 days after the 2nd medicine, reach best control efficiency, the highest preventive effect is respectively 80.68% and 76.73%.Visible, the Be very effective of complex preparation is better than two kinds of single doses, and its quick-acting and lasting effect are all relatively good, especially outstanding with lasting effect.According to field range estimation, within the scope of test dose, plant growth is normal, and the poisoning phenomenon to paddy rice does not all appear in each treatment agent, illustrates that it is safe to paddy rice.
Penconazole, Zhongshengmycin and composite water prevention rice sheath blight effect experiment thereof
(1) test method
Test dispenser twice altogether, check plot spray equivalent clear water.When making up a prescription, after first medicament fully being dissolved with a small amount of water, then add suitable quantity of water and carry out all-out atomizing process, even spraying, spray emphatically rice strain middle and lower part.All adopt power driven sprayer during spray medicine, operating pressure is 2.0 ~ 2.3kg/cm
2, shower nozzle aperture 1.2mm.Community spouting liquid presses 675L/10000m
2use, liquid is sprayed application to uniformly the positive and negative two sides of crop leaf.
(2) investigation method
Experimental field be located at Hunan, the rice field selecting occurring degree medium is that experimental field soil fertility is medium, and irrigation and drainage are convenient.Adopt bilinear sampling method, 5 points are got in every community, look into 5 caves at often, and during investigation, 2 blades in the fixing middle part of every strain carry out fixed point inspection.
Carry out classification according to rice sheath blight disease grade scale, record total strain number, diseased plant number, severity, calculate diseased plant rate, disease index and preventive effect.
Rice sheath blight disease grade scale is
0 grade: complete stool is anosis;
1 grade: 4th blade and following leaf sheath thereof, blade morbidity (be 1st slice leaf with sword-like leave);
3 grades: 3rd blade and following leaf sheath thereof, blade morbidity;
5 grades: 2nd blade and following leaf sheath thereof, blade morbidity;
7 grades: sword-like leave blade and following leaf sheath thereof, blade morbidity;
9 grades: complete stool falls ill, withered ahead of time.
Test is investigated 4 times altogether, i.e. disease index investigation before medicine, preventive effect investigation in 7 days after the 1st medicine, 7 days and preventive effect investigation in 15 days after 2 medicines.
(3) poisoning investigation
Whether after dispenser, continuous 7d estimates medicament has poisoning to crop.
(4) drug effect computational methods
Sick leaf rate (%)=sick number of sheets/investigate the total number of sheets × 100
Disease index=∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=(1-(before blank district medicine after disease index × treatment region medicine disease index)/(after blank district medicine before disease index × treatment region medicine disease index)) × 100
(5) field control effectiveness test result
The experiment effect of the experimental pharmacy process water prevention rice sheath blight respectively organized by table 3
Interpretation of result:
As seen from Table 3, the Be very effective of the mixture combination control rice blast of 20% penconazole Zhongshengmycin WP (1.5:1).After first time medicine 7 days, after second time medicine, 7 days and 15 days preventive effects the highest were respectively 70.03%, 75.33% and 82.23%.25% penconazole EW single dose and 6% Zhongshengmycin WP single dose, all within 15 days after the 2nd medicine, reach best control efficiency, the highest preventive effect is respectively 76.27% and 70.05%.Visible, the Be very effective of complex preparation is better than two kinds of single doses, and its quick-acting and lasting effect are all relatively good, especially outstanding with lasting effect.According to field range estimation, within the scope of test dose, plant growth is normal, and the poisoning phenomenon to paddy rice does not all appear in each treatment agent, illustrates that it is safe to paddy rice.
To sum up described in test, of the present invention with penconazole and Zhongshengmycin for the composite bactericidal composition preparation of active ingredient, to rice blast and Rhizoctonia solani Kuhn all well control efficiency, and it is to Target crops safety, compared with single dose, bactericidal composition of the present invention has the advantage that quick-acting is good, the lasting period is long, and environmental pollution is few, not easily produces poisoning.So research and development of the present invention and popularization are of great significance social tool.
Claims (3)
1. contain a bactericidal composition for penconazole and Zhongshengmycin, it is characterized in that, described bactericidal composition comprises active ingredient penconazole and Zhongshengmycin;
Its formulation of described bactericidal composition is any one in wetting powder, suspending agent, aqueous emulsion, water dispersible granules and missible oil;
When bactericidal composition is wetting powder, the percentage by weight of each component is: penconazole 1% ~ 90%, Zhongshengmycin 1% ~ 90%, dispersant 1% ~ 20%, wetting agent 1% ~ 10%, filler 1% ~ 70%, by above-mentioned weight proportion by penconazole and Zhongshengmycin and the mixing of dispersant, wetting agent and filler, uniform stirring in stirred tank, mix again after airslide disintegrating mill is pulverized, obtain wetting powder bactericidal composition;
When bactericidal composition is suspending agent, the percentage by weight of each component is: penconazole 1% ~ 60%, Zhongshengmycin 1% ~ 60%, dispersant 5% ~ 20%, antifreezing agent 1% ~ 5%, thickener 0.1% ~ 2%, defoamer 0.1% ~ 0.8%, surplus is water, the concrete production stage of this suspending agent is: first mixed by above-mentioned proportioning by auxiliary agent, mix through high speed shear, add penconazole and Zhongshengmycin, abrading-ball 2 ~ 3 hours in ball crusher, make a diameter all at below 5mm, obtain the bactericidal composition of aqueous suspension formulations;
When bactericidal composition is aqueous emulsion, the percentage by weight of each component is: penconazole 1% ~ 60%, Zhongshengmycin 1% ~ 60%, emulsifier 3% ~ 30%, solvent 5% ~ 15%, stabilizing agent 0.2% ~ 15%, antifreezing agent 0.1% ~ 5%, defoamer 0.1% ~ 8%, thickener 0.1% ~ 2%, surplus are water; The concrete production stage of this aqueous emulsion is: penconazole and Zhongshengmycin, solvent and emulsifier are mixed, and makes it to be dissolved into uniform oil phase; Water, antifreezing agent, stabilizing agent, thickener and defoamer are mixed uniformly aqueous phase; While high-speed stirred, oil phase is added aqueous phase in a kettle., slowly add water until reach phase inversion point, open clipper and carry out high speed shear, shear 20 minutes to 1 hour, form the aqueous emulsion of oil-in-water type, obtain the bactericidal composition of aqueous emulsion;
When bactericidal composition is water dispersible granules, the mass percent of component is: penconazole 1% ~ 90%, Zhongshengmycin 1% ~ 90%, dispersant 3% ~ 20%, wetting agent 3% ~ 10%, disintegrant 1% ~ 5%, filler 1% ~ 70%, the concrete production stage of this aqueous emulsion is: penconazole, Zhongshengmycin and dispersant, wetting agent, disintegrant and filler are mixed, pulverize with micro jet, through mediating, then add in fluidized-bed granulation dryer and carry out granulation, drying, screening, obtain the bactericidal composition of water dispersant;
When bactericidal composition is missible oil, the mass percent of component is: penconazole 1% ~ 60%, Zhongshengmycin 1% ~ 60%, emulsifier 1% ~ 15%, surplus is solvent, the concrete production stage of this missible oil is: first penconazole and Zhongshengmycin are added in solvent according to above-mentioned ratio and dissolve completely, then add the oily liquids that emulsifier for mixing evenly becomes transparent and homogeneous afterwards, obtain the bactericidal composition of cream preparation;
Wherein, the mass ratio of penconazole and Zhongshengmycin is any one ratio in 3:1,1.5:1,1:1,1:1.5,1:3.
2. bactericidal composition according to claim 1, is characterized in that:
Described dispersant is: at least one in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, fatty acid glyceride APEO;
Described wetting agent is: at least one in lauryl sodium sulfate, calcium dodecyl benzene sulfonate, Nekal BX, wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene tri-styryl phosphenylic acid salt, spaonin powder, silkworm excrement, soapberry powder;
Described disintegrant is: at least one in bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, sodium bicarbonate;
Described emulsifier is: the mixed solvent that any one or more and calcium dodecyl benzene sulfonate in aliphatic acid polyethenoxy ether, alkylphenol polyoxyethylene sulfosuccinate, styrylphenol polyoxyethylene ether, polyoxyethylene nonylphenol ether, castor oil polyoxyethylene ether, aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether form with arbitrary proportion;
Described solvent is any one or more mixed solvents formed with arbitrary proportion in dimethylbenzene, toluene, diesel oil, methyl alcohol, ethanol, n-butanol, isopropyl alcohol, turpentine oil, solvent naphtha, dimethyl formamide, dimethyl sulfoxide (DMSO), aqueous solvent;
Described filler is: at least one in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, precipitated calcium carbonate;
Described thickener is: at least one in xanthans, CMC, hydroxyethylcellulose, methylcellulose, Magnesiumaluminumsilicate, polyvinyl alcohol;
Described antifreezing agent is: at least one in ethylene glycol, propane diols, glycerine, polyethylene glycol;
Described stabilizing agent is: at least one in sodium citrate, resorcinol;
Described defoamer is: at least one in silicone oil, silicone compound, C10-20 saturated fat acid compounds, C8-10 aliphatic alcohols compound.
3. bactericidal composition according to claim 1 is for preventing and treating the application of rice blast and rice sheath blight disease.
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| CN106172453B (en) * | 2015-04-29 | 2018-07-03 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition and its application |
| CN105541822B (en) * | 2016-01-05 | 2018-11-27 | 贵州大学 | Evil containing 1,3,4-（Thiophene）Pyridine salt compounds of di azoly and preparation method and application |
| CN108887279A (en) * | 2018-06-06 | 2018-11-27 | 沈阳工业大学 | A kind of bactericidal composition containing Zhongshengmycin and Flutriafol and application |
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| DE4318285A1 (en) * | 1993-06-02 | 1994-12-08 | Bayer Ag | Fungicidal active ingredient combinations |
| CN1219879C (en) * | 2003-11-04 | 2005-09-21 | 中国农业科学院生物防治研究所 | Hainan variety of lilac grey streptomycete and method for preparing agricultural antibiotic from variety |
| CN1279827C (en) * | 2005-08-25 | 2006-10-18 | 齐军山 | Agricultural sterilizing composition aimed at plant disease |
| CN101180962B (en) * | 2007-12-17 | 2012-04-04 | 东莞市瑞德丰生物科技有限公司 | Composition containing Zhongshengmycin and zineb with synergism |
| CN101496522B (en) * | 2009-02-26 | 2012-09-19 | 陕西韦尔奇作物保护有限公司 | Bactericidal composition containing Zhongshengmycin and tebuconazole as well as use thereof |
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Inventor after: Fan Kun Inventor after: Guo Qinglong Inventor after: Bei Feng Inventor after: Xu Meiling Inventor after: Liu Runzhu Inventor after: Wang Yaen Inventor after: Cui Shuhua Inventor before: Cui Shuhua Inventor before: Guo Qinglong Inventor before: Bei Feng Inventor before: Xu Meiling Inventor before: Liu Runzhu Inventor before: Wang Yaen |
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