CN105767005A - Application of amino-oligosaccharin and lactose high-temperature product in aspect of preventing and treating peanut bacterial wilt - Google Patents
Application of amino-oligosaccharin and lactose high-temperature product in aspect of preventing and treating peanut bacterial wilt Download PDFInfo
- Publication number
- CN105767005A CN105767005A CN201610220128.8A CN201610220128A CN105767005A CN 105767005 A CN105767005 A CN 105767005A CN 201610220128 A CN201610220128 A CN 201610220128A CN 105767005 A CN105767005 A CN 105767005A
- Authority
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- China
- Prior art keywords
- lactose
- amino
- temperature product
- bacterial wilt
- preventing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Abstract
The invention relates to application of amino-oligosaccharin and a lactose high-temperature product in the aspect of preventing and treating peanut bacterial wilt.The lactose high-temperature product is obtained by mixing lactose and water according to the mass ratio of 3:10 to be prepared into a lactose solution, then placing the lactose solution into a high-temperature and high-pressure sterilization pot to be treated at the temperature of 120 DEG C for 30 min, and performing cooling.The mass ratio of the lactose high-temperature product to the amino-oligosaccharin is 1:1-5.The amino-oligosaccharin and the lactose high-temperature product are remarkable in synergistic effect and can remarkably prevent peanut bacterial wilt.
Description
Technical field
The present invention relates to control of crop disease technical field, specifically amino-oligosaccharide withered preventing and treating Semen arachidis hypogaeae green grass or young crops with lactose high-temperature product
Application in terms of Bing.
Background technology
Amino-oligosaccharide, the most agriculture special oligochitosan, is to be obtained after chitosan degraded again by chitin degrading to prepare, or by micro-
The low toxicity antibacterial that biofermentation extracts.Amino-oligosacchride cellulose solution has virus killing, bactericidal, fungicidal action, can be widely used for
Preventing and treating fruit tree, vegetable, underground rhizome, Nicotiana tabacum L., Chinese crude drug and the virus of grain and cotton crop, antibacterial, fungus-caused mosaic disease,
The diseases such as little leaf, spot disease, anthrax, downy mildew, epidemic disease, blight dis-ease, BYDV, rice blast, bacterial wilt, soft rot
Evil.But the alone less effective to bacterial wilt of peanut of amino-oligosaccharide at present.
Bacterial wilt of peanut is a kind of bacterial disease based on soil-borne caused by Ralstonia solanacearum, is to endanger in the world
Maximum, distribution are the most extensively, cause damage one of the most serious plant disease, is referred to as " cancer " of plant.At present this disease is prevented
Control main based on chemical means, but use chemical pesticide for a long time in a large number and be easily generated Drug resistance and cause environmental pollution, therefore, as
On the basis of where ensureing bacterial wilt prevention effect, reduce the use of chemical pesticide, reduce environmental pollution, be that bacterial wilt of peanut is prevented
Control major issue to be solved in field.
Summary of the invention
The present invention is directed to the problem that the preventing and treating of above-mentioned bacterial wilt of peanut exists, it is provided that amino-oligosaccharide and lactose high-temperature product are at preventing and treating flower
Application in terms of raw bacterial wilt, improves the prevention effect of peanut solanacearum.
The present invention realizes above-mentioned purpose and is adopted the technical scheme that: amino-oligosaccharide and lactose high-temperature product are withered preventing and treating Semen arachidis hypogaeae green grass or young crops
Application in terms of Bing, described lactose high-temperature product is to be mixed according to the mass ratio of 3:100 with water by lactose, is configured to lactose molten
Liquid, then puts into lactose solution in autoclave sterilization pot, and 120 DEG C process 30min, gained after cooling；
Described lactose high-temperature product is 1:1-5 with the mass ratio of amino-oligosaccharide.
Described lactose high-temperature product is 1:4 with the mass ratio of amino-oligosaccharide.
Synergism of the present invention is notable, it is possible to the notable generation preventing bacterial wilt of peanut.
Detailed description of the invention
Below in conjunction with test data and embodiment, technical scheme and beneficial effect are described further.Of the present invention
Lactose high-temperature product be that lactose is mixed according to the mass ratio of 3:100 with water, be configured to lactose solution, then by lactose solution
Putting in autoclave sterilization pot, 120 DEG C process 30min, gained after cooling.The lactose high-temperature product of gained is considered as 100%
Female medicine.
One Toxicity Determination test
(1) medicament is equipped with:
Above-mentioned lactose high-temperature product is mixed according to 1:0.5,1:1,1:2,1:3,1:4,1:5,1:6 with amino-oligosaccharide,
Obtain mix preparation 1#-7#.
(2) toxicity test method:
Test uses nephelometry, measures above-mentioned lactose high-temperature product single dose, amino-oligosaccharide single dose, 1#-7# to peanut solanacearum
Virulence.Assay method particularly as follows: by lactose high-temperature product single dose, amino-oligosaccharide single dose, 1#-7# respectively according to 200ppm,
The concentration of 100ppm, 50ppm, 25ppm, 12.5ppm is injected peanut solanacearum (Peanut bacterial wilt) and is hanged
In liquid, by according to concentration inject in peanut solanacearum (Peanut bacterial wilt) suspension, put into 30 ± 1 DEG C of cultivations
In case, cultivate 48 hours, take out and measure its turbidity (making reference with the comparison of injected clear water), calculate it to bacterial wilt of peanut
The EC of bacterium50, evaluating the potentiation of pharmacy mix, i.e. CTC≤80 according to co-toxicity coefficient method (CTC) is antagonism,
80<CTC<120 is summation action, and CTC>=120 are potentiation.
(3) interpretation of result:
| Reagent agent | EC50(ppm) | Actual measurement toxicity index | Theoretical toxicity index | Co-toxicity coefficient |
| Lactose high-temperature product | 365.12 | 3.41 | / | / |
| Amino-oligosaccharide | 12.45 | 100.00 | / | / |
| 1#(1:0.5) | 27.81 | 44.77 | 68.94 | 64.9 |
| 2#(1:1) | 8.92 | 139.57 | 101.70 | 137.2 |
| 3#(1:2) | 9.17 | 135.77 | 101.14 | 134.2 |
| 4#(1:3) | 9.02 | 138.03 | 100.85 | 136.9 |
| 5#(1:4) | 8.42 | 147.86 | 100.00 | 147.9 |
| 6#(1:5) | 11.47 | 108.54 | 83.90 | 129.4 |
| 7#(1:6) | 20.95 | 59.43 | 86.20 | 68.9 |
Upper table is each medicament Toxicity Determination result statistical table to bacterial wilt of peanut.As can be known from the above table, described lactose high temperature
When product and amino-oligosaccharide mass ratio are 1:1-5, synergistic results is notable, then shows that antagonism is made when mass ratio is 1:0.5 and 1:6
With.
Two field medicine effect tests
1. trial crops: select first crop bacterial wilt to occur the most serious Peanut continuous cropping as subjects.
2. test processes and arranges:
3 spraying treatments are set: 300 times of liquid of 5# medicament, 3% amino-oligosaccharide 300 times of liquid, clear water.
Above-mentioned spray liquid is sprayed before Semen arachidis hypogaeae onset peak in seedling stage, and Semen arachidis hypogaeae full-bloom stage terminates to carry out bacterial wilt incidence survey, receives
The phase of obtaining carries out determination of yield, using clear water as comparison, calculates the Rectifying efficacies of each process bacterial wilt of peanut, calculates each process simultaneously
Increase of production rate.
3. result of the test such as following table:
| Test processes | Rectifying efficacies (%) to bacterial wilt of peanut | Yield rate of growth (%) |
| 5# medicament | 95.33 | 65.17 |
| Amino-oligosacchride | 67.15 | 28.46 |
Finding out from field experiment, using 5# of the present invention to prevent bacterial wilt of peanut, effect is substantially than amino-oligosacchride
Element single dose to be got well, and increase of production also becomes apparent from.
Claims (2)
1. amino-oligosaccharide and the lactose high-temperature product application in terms of preventing and treating bacterial wilt of peanut, it is characterised in that described lactose
High-temperature product is to be mixed according to the mass ratio of 3:100 with water by lactose, is configured to lactose solution, then lactose solution is put into height
In temperature high-pressure sterilizing pot, 120 DEG C process 30min, gained after cooling；
Described lactose high-temperature product is 1:1-5 with the mass ratio of amino-oligosaccharide.
Compositions containing lactose high-temperature product and amino-oligosaccharide the most according to claim 1 is in terms of preventing and treating bacterial wilt of peanut
Application, it is characterised in that the mass ratio of described lactose high-temperature product and amino-oligosaccharide is 1:4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610220128.8A CN105767005A (en) | 2016-04-08 | 2016-04-08 | Application of amino-oligosaccharin and lactose high-temperature product in aspect of preventing and treating peanut bacterial wilt |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610220128.8A CN105767005A (en) | 2016-04-08 | 2016-04-08 | Application of amino-oligosaccharin and lactose high-temperature product in aspect of preventing and treating peanut bacterial wilt |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105767005A true CN105767005A (en) | 2016-07-20 |
Family
ID=56396209
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610220128.8A Pending CN105767005A (en) | 2016-04-08 | 2016-04-08 | Application of amino-oligosaccharin and lactose high-temperature product in aspect of preventing and treating peanut bacterial wilt |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105767005A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CN104430390A (en) * | 2014-10-24 | 2015-03-25 | 青岛瀚生生物科技股份有限公司 | Amino-oligosaccharin and ethaboxam compounded agricultural fungicide |
| CN105191940A (en) * | 2015-08-25 | 2015-12-30 | 江苏科技大学 | Application of chitosan derivative in bacterial wilt prevention and treatment |
-
2016
- 2016-04-08 CN CN201610220128.8A patent/CN105767005A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CN104430390A (en) * | 2014-10-24 | 2015-03-25 | 青岛瀚生生物科技股份有限公司 | Amino-oligosaccharin and ethaboxam compounded agricultural fungicide |
| CN105191940A (en) * | 2015-08-25 | 2015-12-30 | 江苏科技大学 | Application of chitosan derivative in bacterial wilt prevention and treatment |
Non-Patent Citations (2)
| Title |
|---|
| 余本水: "《高毒农药替代品应用技术指南》", 31 October 2008, 中国农业出版社 * |
| 朱程玉: "乳糖在无菌工艺模拟试验中的应用及清洗验证", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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Application publication date: 20160720 |
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