CN106818807A - A kind of composite bactericide for preventing and treating banana freckle - Google Patents
A kind of composite bactericide for preventing and treating banana freckle Download PDFInfo
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- CN106818807A CN106818807A CN201710119412.0A CN201710119412A CN106818807A CN 106818807 A CN106818807 A CN 106818807A CN 201710119412 A CN201710119412 A CN 201710119412A CN 106818807 A CN106818807 A CN 106818807A
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- China
- Prior art keywords
- pyraclostrobin
- banana
- rosickyite azoles
- composite bactericide
- active compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of composite bactericide for preventing and treating banana freckle disclosed by the invention, is with rosickyite azoles and pyraclostrobin as base-material, according to the composite bactericide that the quality proportioning 1: 3~3: 1 of rosickyite azoles and OIT is compounded；The rosickyite azoles is the active compound of mass fraction 98.9%, and the pyraclostrobin is that mass fraction is 97.5% pyraclostrobin active compound.The composite bactericide of preventing and treating banana freckle of the invention, after two kinds of base-material mixtures, with obvious synergy or addition effect, use safety, one of the disease for generally occur for the Banana Growth later stage, influenceing Banana Industry most serious Phyllosticta musarum has a good cytotoxicity, and apparently higher than rosickyite azoles or pyrazoles ether bacterium vinegar single dose.It is used to prevent and treat banana freckle suitable for vast rural area.
Description
Technical field
The present invention is relevant with bactericide, specifically, belongs to the bactericide containing organic compound, furthermore, it is understood that belonging to
Prevent and treat the bactericide of banana freckle.The built agent also have to blade permeate the biography effect of seeking, it is water-fast to wash away, to wheat, Dao Shui,
The sac fungus of peanut, grape, vegetables, potato, lemon, coffee, walnut, fruit tree, tealeaves, tobacco and ornamental plant etc., load
Multiple diseases caused by bacterium, oomycetes, Fungi Imperfecti, such as：Leaf spot, powdery mildew, leaf blight, rust, net blotch, epidemic disease, downy mildew
Disease, rice blast, banded sclerotial blight, corn northern and southern leaf blight have protection and therapeutic action.
Technical background
Banana freckle, also known as black spot, cause of disease is Deuteromycotina Macrophoma musae, the germ of any of several broadleaf plants garden dead leaf stubble
Conidium is this sick primary infection source.Harm blade and any of several broadleaf plants are really.Banana freckle be generally occur in the Banana Growth later stage,
Influence one of disease of Banana Industry most serious.Grave illness blade black splotch is intensive, causes blade withered and yellow, wilting, on susceptible fruit
The commodity value of close raw black splotch, influence banana outward appearance and reduction fruit.Good protective agents are there is no at present.
Rosickyite azoles is Albendazole (Albendazole), is a kind of efficient, safety, broad spectrum activity imidazoles series bactericidal agent,
It is disinfectant use in agriculture that China has independent intellectual property right, has significant preventive effect to plurality of plant diseases, and have certain volume increase
Effect；But after using for many years, germ gradually generates the resistance to the action of a drug, causes drug effect to reduce.(Pyraclostrobin) it is one
New-type wide-spectrum bactericide is planted, with protection, treatment, blade infiltration conduction.To cucumber, banana safety, have no that poisoning occurs.
It is related to the application part for preventing and treating banana freckle (black spot) little in Chinese patent database, only：
No. 2015103865101《A kind of prevention and controls of banana freckle》, No. 2016106898629《One kind prevention banana black spot
Fertilizer》.The application part for compounding preventing and treating banana freckle bactericide with pyraclostrobin with albendazole is there is no at present.
The content of the invention
The present invention is intended to provide a kind of composite bactericide for preventing and treating banana freckle, to slow down pathogen to banana crops
Harm, improves banana presentation quality.
Inventor carries out repeated screening to the agricultural chemicals with killing Deuteromycotina Macrophoma musae bacterium, and to mixture medicine
The bulk drug quality proportioning of agent carries out repetition test, and result of the test is contrasted, and finds a kind of compound disinfectant, black to banana
Star disease germ possesses stronger poisoning ability.
The composite bactericide of the preventing and treating banana freckle that inventor provides is, with rosickyite azoles and OIT as base-material, to press
According to the composite bactericide of the compounding of quality proportioning 1: 3~3: 1 of rosickyite azoles and pyraclostrobin, above-mentioned different ratio is answered
Screening test is carried out with bactericide；According to experimental result, inventor determines, with rosickyite azoles and pyraclostrobin as base-material is compounded
Into composite bactericide, the co-toxicity coefficient to banana freckle in the range of 1: 3~3: 1 is all higher than 120, illustrates that two medicaments are answered
The preventing and treating for matching banana freckle has synergistic effect；Wherein pyraclostrobin, rosickyite azoles active ingredient ratio are 1:When 1, altogether
Malicious coefficient highest, prevention effect is best.
The rosickyite azoles is the active compound of mass fraction 98.9%, is produced by Guizhou Daoyuan Biotechnology Co., Ltd.；It is described
97.5% pyraclostrobin active compound, is provided by Zhejiang Boshida Crop Technology Co., Ltd..
The screening test of the composite bactericide raw material proportioning of above-mentioned preventing and treating banana freckle is as follows：
1 materials and methods
1.1 reagent agents：
Major pharmaceutical agent：A (rosickyite azoles), B (pyraclostrobin)；
Strains tested：Banana freckle (Macrophoma musae), plant pathology teaching and research room of Agricultural University Of Hunan separates
Purification storage is simultaneously provided.
For examination culture medium：Banana freckle (Macrophoma musae),LakeSouthern agriculture university's plant pathology teaching and research room point
From purification storage and provide.
1.2 condition of culture
Culture, 25 DEG C ± 1 DEG C of temperature, relative humidity 80%~90% in bio-incubator.
1.3 key instrument equipment
Electronic balance (sensibility reciprocal 0.1mg), sprayer, bio-incubator, diameter 9cm culture dishes, pipette, inoculator, beat
Hole device, chi long etc..
2. toxicity test method
With reference to the Plating that People's Republic of China's agricultural industry criteria farm-chemical indoor determination test rule is recommended
(NY/T 1156.2-2006).Step is as follows：(1) indoor culture banana freckle is for inoculation.(2) medicament configuration：Will be appropriate
Pyraclostrobin, rosickyite azoles active compound are dissolved with 2ml acetone and glacial acetic acid respectively, then are distinguished with 0.5% Tween 80 aseptic aqueous solution
The mother liquor for preparing 1000mg/L is stand-by.(3) chemicals treatment：Aseptically, the sterilizing that will be melted in advance according to test process
PDA culture medium is quantitatively adding in aseptic conical flask, is quantitatively drawn liquid successively from low concentration to high concentration and is separately added into above-mentioned cone
In shape bottle, fully shake up, then equivalent is poured into 4 culture dishes of a diameter of 9cm and is made pastille flat board, wherein pyraclostrobin
Concentration is 2,4,8,16,32mg/kg, the concentration of rosickyite azoles is 2,4,8,16,32mg/kg；5 pyraclostrobins and rosickyite azoles are not
With proportioning, (the ratio between active ingredient is respectively 1: 3；1∶2；1∶1；2∶1；3: 1) first dissolved with dichloromethane respectively, then with containing
The sterilized water of 0.1% Tween-80 is diluted to 1.5,3,6,12,24mg/kg series concentrations it is stand-by.(4) inoculation and culture：Will culture
Good bacteria cake, aseptically with the sterilization punchers of a diameter of 5mm, bacteria cake is cut from colony edge, and bacterium is seen with inoculator
Cake is inoculated in pastille flat board center, and mycelia faces up, and covers ware lid, is cultivated in incubator, each 4 repetition for the treatment of, if 0.5%
Tween-80 aseptic aqueous solution is compareed.(5) investigate：Growing state investigation bacterium germ silk according to bacterium in blank culture dish is raw
Situation long, with kind of calliper colony diameter (mm), each bacterium colony is each once with crossing method vertical survey diameter, takes its average
Value.Correlation computations formula is：
D=D1-D2
D is that bacterium colony increases diameter；D1It is colony diameter；D2It is bacteria cake diameter
I=100 × (D0-Dt)/D。
I is mycelial growth inhibition rate,
D0For blank bacterium colony increases diameter；
Then virulence regression equation is sought with DPS software statistics, calculates EQ50, EC90 and 97.5% confidence of each medicament
Limit.
3 result of the tests
The different mixture preparations of pyraclostrobin, rosickyite azoles and 5 are entered using the above method to Phyllosticta musarum respectively
Row toxicity test simultaneously carries out statistical analysis, and it the results are shown in Table 1, from data in table, pyraclostrobin, 5 differences of rosickyite azoles
With compare banana freckle co-toxicity coefficient be all higher than 120, illustrate that two composite reagents have synergistic effect to banana freckle.
Wherein, when pyraclostrobin, rosickyite azoles active ingredient ratio are 1: 1, co-toxicity coefficient highest is 149.15.
The pyraclostrobin of table 1, rosickyite azoles and 5 different ratios are to banana freckle toxicity test result
Dt is that chemicals treatment bacterium colony increases diameter
4 discussion of results
Toxicity Determination result shows that 5 different proportion mixtures of pyraclostrobin and rosickyite azoles are to Phyllosticta musarum
Co-toxicity coefficient be all higher than 120, illustrate above-mentioned two composite reagent for prevent and treat banana freckle be it is feasible, select pyrazoles ether
To carry out compounding effect optimal for 1: 1 ratio for the ratio between bacterium ester and rosickyite azoles effective ingredient.
The composite bactericide of preventing and treating banana freckle of the invention, after two kinds of base-material mixtures, with obvious synergy or
Person is added effect, and using safety, one of the disease for generally occur for the Banana Growth later stage, influenceing Banana Industry most serious banana is black
Star germ has a good cytotoxicity, and apparently higher than rosickyite azoles or pyrazoles ether bacterium vinegar single dose.It is used to prevent suitable for vast rural area
Control banana freckle.
Specific embodiment
Embodiment 1
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98.9% rosickyite azoles active compound 2.0kg, then
The mass fraction for taking Zhejiang Boshida Crop Technology Co., Ltd.'s production is 97.5% pyraclostrobin active compound 6.0kg, is re-dubbed
8.0kg prevents and treats the composite bactericide of banana freckle.
Embodiment 2
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98.9% rosickyite azoles active compound 2.0kg, then
The mass fraction for taking Zhejiang Boshida Crop Technology Co., Ltd.'s production is 97.5% pyraclostrobin active compound 4.0kg, is re-dubbed
6.0kg prevents and treats the composite bactericide of banana freckle.
Embodiment 3
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98.9% rosickyite azoles active compound 2.0kg, then
The mass fraction for taking Zhejiang Boshida Crop Technology Co., Ltd.'s production is 97.5% pyraclostrobin active compound 2.0kg, is re-dubbed
4.0kg prevents and treats the composite bactericide of banana freckle.
Embodiment 4
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98.9% rosickyite azoles active compound 4.0kg, then
The mass fraction for taking Zhejiang Boshida Crop Technology Co., Ltd.'s production is 97.5% pyraclostrobin active compound 2kg, is re-dubbed
6.0kg prevents and treats the composite bactericide of banana freckle.
Embodiment 5
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98.9% rosickyite azoles active compound 6.0kg, then
The mass fraction for taking Zhejiang Boshida Crop Technology Co., Ltd.'s production is 97.5% pyraclostrobin active compound 2kg, is re-dubbed
8.0kg prevents and treats the composite bactericide of banana freckle.
Claims (2)
1. it is a kind of prevent and treat banana freckle composite bactericide, it is characterised in that the composite bactericide is with rosickyite azoles and pyrazoles
Kresoxim-methyl is base-material, according to the composite bactericide that the quality proportioning 1: 3~3: 1 of rosickyite azoles and OIT is compounded；Institute
The active compound that rosickyite azoles is mass fraction 98.9% is stated, the pyraclostrobin is that mass fraction is 97.5% pyraclostrobin active compound.
2. it is a kind of prevent and treat rice blast composite bactericide, it is characterised in that the composite bactericide is with rosickyite azoles and isothiazole
Quinoline ketone is base-material, according to the composite bactericide that the quality proportioning 1: 1 of rosickyite azoles and OIT is compounded；The rosickyite azoles
It is the active compound of mass fraction 98.9%, the pyraclostrobin is that mass fraction is 97.5% pyraclostrobin active compound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710119412.0A CN106818807A (en) | 2017-03-02 | 2017-03-02 | A kind of composite bactericide for preventing and treating banana freckle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710119412.0A CN106818807A (en) | 2017-03-02 | 2017-03-02 | A kind of composite bactericide for preventing and treating banana freckle |
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| Publication Number | Publication Date |
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| CN106818807A true CN106818807A (en) | 2017-06-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710119412.0A Withdrawn CN106818807A (en) | 2017-03-02 | 2017-03-02 | A kind of composite bactericide for preventing and treating banana freckle |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333791A (en) * | 2017-08-30 | 2017-11-10 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of the preventing and treating rice blast of OIT compounding |
| CN107736366A (en) * | 2017-11-28 | 2018-02-27 | 镇江宝成生物科技有限公司 | A kind of prevention and controls of banana freckle |
| CN111802396A (en) * | 2020-08-11 | 2020-10-23 | 四川农业大学 | Bactericidal composition and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1258192A (en) * | 1997-05-30 | 2000-06-28 | 巴斯福股份公司 | Fungicidal mixtures |
| CN105407721A (en) * | 2013-07-31 | 2016-03-16 | 龙灯农业化工国际有限公司 | Fungicidal compositions and their use |
-
2017
- 2017-03-02 CN CN201710119412.0A patent/CN106818807A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1258192A (en) * | 1997-05-30 | 2000-06-28 | 巴斯福股份公司 | Fungicidal mixtures |
| CN105407721A (en) * | 2013-07-31 | 2016-03-16 | 龙灯农业化工国际有限公司 | Fungicidal compositions and their use |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107333791A (en) * | 2017-08-30 | 2017-11-10 | 贵州道元生物技术有限公司 | By rosickyite azoles and the bactericide of the preventing and treating rice blast of OIT compounding |
| CN107736366A (en) * | 2017-11-28 | 2018-02-27 | 镇江宝成生物科技有限公司 | A kind of prevention and controls of banana freckle |
| CN111802396A (en) * | 2020-08-11 | 2020-10-23 | 四川农业大学 | Bactericidal composition and application thereof |
| CN111802396B (en) * | 2020-08-11 | 2022-04-26 | 四川农业大学 | Bactericidal composition and application thereof |
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Application publication date: 20170613 |