CN106889091A - A kind of composite bactericide for preventing and treating sigatoka - Google Patents
A kind of composite bactericide for preventing and treating sigatoka Download PDFInfo
- Publication number
- CN106889091A CN106889091A CN201710119427.7A CN201710119427A CN106889091A CN 106889091 A CN106889091 A CN 106889091A CN 201710119427 A CN201710119427 A CN 201710119427A CN 106889091 A CN106889091 A CN 106889091A
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- China
- Prior art keywords
- fluoxastrobin
- sigatoka
- rosickyite azoles
- composite bactericide
- preventing
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
Abstract
A kind of composite bactericide for preventing and treating sigatoka disclosed by the invention, the composite bactericide is with Fluoxastrobin and rosickyite azoles as base-material, the composite bactericide that quality proportioning 1: 1~1: 8 according to Fluoxastrobin and rosickyite azoles is compounded, the Fluoxastrobin is the active compound of mass fraction 98%；The rosickyite azoles is 98% active compound.Preferably, the composite bactericide is, with Fluoxastrobin and rosickyite azoles as base-material, to be formed according to the compounding of quality proportioning 1: 4~1: 8 of Fluoxastrobin and rosickyite azoles.The composite bactericide for preventing and treating sigatoka of the invention, after two kinds of base-material mixtures, effect is substantially added, use safety, one of the disease for generally occur for the Banana Growth later stage, influenceing Banana Industry most serious sigatoka bacterium has a good cytotoxicity, and apparently higher than Fluoxastrobin and rosickyite azoles single dose.It is used to prevent and treat sigatoka suitable for vast rural area.
Description
Technical field
The present invention is relevant with bactericide, specifically, belongs to the bactericide containing organic compound, furthermore, it is understood that belonging to
Prevent and treat the bactericide of sigatoka.To disease such as powdery mildew, rust, glume blight, net blotch, the downy mildew of nearly all mycota
Disease, rice blast etc. have good activity.
Technical background
Sigatoka, generally occurs in south China banana producing region, common are sigatoka leaf spot disease, grey line disease and coal line disease,
Wherein sigatoka leaf spot disease is the most serious.In the plant disease epidemic time, blade injured area is 20%~40%, when serious up to 80% with
On, diseased plant yield is reduced, and fruit quality declines, the underproduction more than 50% when serious.Original control prece was made in emphasis period
With《Beautiful fruit peace》Liquid is sprayed, or is used《It is fast net》+《Garlic oil》Preventing and treating.More preferable protective agents are there is no at present.
Fluoxastrobin (Azoxystrobin), is methoxy acrylate (Strobilurin) series bactericidal agent, efficient, wide spectrum,
To almost all of mycota (Ascomycotina, Basidiomycotina, Mastigomycotina and Deuteromycotina) disease such as powdery mildew,
Rust, glume blight, net blotch, downy mildew, rice blast etc. have good activity.Rosickyite azoles, chemical name be 5- (rosickyite base)-
1H- benzimidazolyl-2 radicals-base-methyl carbamate, is low toxicity, wide spectrum, absorbability benzimidazole germicide.With protecting and control
Treatment is acted on.
Being related to the application part for preventing and treating sigatoka in Chinese patent database has：No. ZL991029887《Olefin conversion emulsion
Prevent and treat the application of sigatoka》, No. 011147059《It is a kind of prevent and treat sigatoka, anthracnose, the agricultural chemicals of leaf spot and its
Preparation method》, No. ZL200810032232X《With the composition for killing harmful eumycetes such as sigatoka effect》、
No. 2008100358694《A kind of composition for preventing and treating harmful eumycetes such as sigatoka》, No. ZL2011800367547《It is fragrant
The method of control of any of several broadleaf plants leaf spot》, No. 2012100248084《A kind of bactericide for preventing and treating sigatoka》With
No. 2014106880429《A kind of bactericidal composition for preventing and treating sigatoka》.There is no at present and compounded with rosickyite azoles with Fluoxastrobin
Prevent and treat the application part of sigatoka bactericide.
The content of the invention
The present invention is intended to provide a kind of composite bactericide for preventing and treating sigatoka compounded with Fluoxastrobin and rosickyite azoles, with
Slow down harm of the pathogen to banana crops, improve banana production and quality.
Inventor carries out repeated screening to the agricultural chemicals for belonging to fungi with killing leaf spot, and to the bulk drug matter of Mixed Pharmacy
Amount proportioning carries out repetition test, and result of the test is contrasted, and finds a kind of compound disinfectant, possesses to sigatoka germ
Stronger poisoning ability.
The composite bactericide for preventing and treating sigatoka that inventor provides is with Fluoxastrobin and rosickyite azoles as base-material, according to phonetic
The composite bactericide that bacterium ester is compounded with the quality proportioning 1: 1~1: 8 of rosickyite azoles.To the composite bactericide of above-mentioned different ratio
Carry out screening test；According to experimental result, inventor determines, with the compound sterilizing that Fluoxastrobin and rosickyite azoles are compounded as base-material
Agent, the preventing and treating to sigatoka in the range of quality proportioning 1: 1~1: 8 has synergistic effect；Wherein Fluoxastrobin and rosickyite
During quality proportioning 1: 1~1: 8 of azoles, prevention effect is preferable.
The Fluoxastrobin is the active compound of mass fraction 98%, is produced by Zhejiang Heben Science ＆ Technology Co., Ltd.；The rosickyite azoles
It is 98% active compound, is produced by Guizhou Daoyuan Biotechnology Co., Ltd..
The screening test of the above-mentioned composite bactericide raw material mass mixture ratio for preventing and treating sigatoka is as follows：
1. test certainly
With sigatoka bacterium as target, using mycelial growth rate method, the optimal of Fluoxastrobin rosickyite azoles compounding is determined
Ratio.
2. experimental condition
2.1 for examination target
Sigatoka bacterium (Mycosphaerellafijiensis), is stored in 4 DEG C of refrigerators.
2.2 condition of culture
The sigatoka bacterium grown in picking test tube, mycelia fragment is ground to form in the broken glass pipe of frosted, is then coated on
On AEA culture medium flat plates, pre- training 10d at 25 DEG C, planar surface equably grows sigatoka bacterium, so can be each from flat board
Mycelia block is produced in position, and mycelia block size can reach diameter 5mm, and cell age is consistent.The bacteria growing is very slow.
2.3 instrument and equipments
The double two-sided clean work station of LRH-250 illumination boxs, SW-CJ-2F, and eppendorf pipettors, baking
Case, card punch, culture dish etc..
3. experimental design
3.1 test medicines
Fluoxastrobin active compound, effective content 98% is provided by Zhejiang Heben Science ＆ Technology Co., Ltd.；
Rosickyite azoles active compound, effective content 98% is provided by Guizhou Daoyuan Biotechnology Co., Ltd..
3.2 test process
3.2.1 dosage is set
Reagent agent Fluoxastrobin methyl alcohol is made into 2000 μ g/ml mother liquors；It is female that rosickyite azoles 0.2M HC1 are made into 4000 μ g/ml
Liquid.Fluoxastrobin, rosickyite azoles are compounded with 1: 1,1: 2,1: 4,1: 6,1: 8 five ratio respectively.Medicament is respectively by institute after pilot study
The series concentration of design is made pastille flat board in being added to AEA culture mediums.The measure concentration of Fluoxastrobin be 5,1.25,0.3125,
0.078、0.02、0.005μ/ml；The measure concentration of rosickyite azoles is 10,5,2.5,1.25,0.625 μ g/ml.Fluoxastrobin rosickyite
During the measure concentration of azoles built agent is shown in Table.If without the control of medicine flat board.The AEA culture mediums of each treatment include that blank contains 50 μ g/
The bigcatkin willow oxime acid of ml suppresses bypass oxidative pathway.
3.2.2 experiment is repeated
Each concentration treatment repeats 4 wares.
4. test method
Bian mycelial growth rate methods.The previously prepared inoculated by hypha block of picking is in the AEA culture mediums of different agents concentration
On flat board, 22d is cultivated at 25 DEG C, check colony diameter, the percentage that each chemicals treatment suppresses mycelial growth is calculated, by suppression
Linear regression analysis between the logarithm value of the probit value of rate processed and serial drug concentration, obtains the EC of each medicament5., according to
Wadley methods calculate the synergistic effect of mixed agent, i.e. SR<0.5, then two kinds of medicament mixtures have an antagonism, 0.5≤SR≤
1.5, then two kinds of medicament mixtures have summation action, SR>1.5, then two kinds of medicament mixtures have synergistic effect.
5. data survey and statistical analysis
The toxicity test of 5.1 Fluoxastrobins, rosickyite azoles single dose to sigatoka bacterium
5.1.1 the virulence of Fluoxastrobin
Inhibition of the Fluoxastrobin of table 1 to sigatoka bacterium mycelial growth
As can be seen from Table 1, the dose-effect curve regression equation that Fluoxastrobin suppresses sigatoka bacterium mycelial growth is Y=
5.756+0.5544X, coefficient correlation is r=0.99, according to regression equation calculation ES50It is 0.0433 μ g/ml.
5.1.2 the virulence of rosickyite azoles
Inhibition of the rosickyite azoles of table 2 to sigatoka bacterium mycelial growth
From table 2 it can be seen that the dose-effect curve regression equation that rosickyite azoles suppresses sigatoka bacterium mycelial growth is Y
=4.7053+0.985IX, coefficient correlation is r=0.99, and EC is obtained according to regression equation calculation50It is 1.9917 μ g/ml.
Toxicity test of the 5.2 Fluoxastrobin rosickyite azoles built agents to sigatoka bacterium
Mycelial growth percentage and virulence regression equation are suppressed according to each medicament single dose and built agent, each compounded combination it is mutual
It is calculated as follows as effect.
5.3.1 Fluoxastrobin rosickyite azoles (1: 1) compounds the inhibition to sigatoka bacterium mycelial growth
From Fluoxastrobin known to Tables 1 and 2 and rosickyite azoles single dose to the EC of sigatoka bacterium50Value is respectively 0.0433 μ g/
Ml and 1.9917 μ g/ml, according to two kinds of virulence of medicament, the theoretical EC after 1: 1 compounding50Value should be：
EC50(theoretical value=1/ [(1/2)/0.0433+ (1/2)/1.9917]=0.0848 μ g/ml
SR50=EC50(theoretical value)/EC5D(observed value)=0.0848/0.872=0.97
5.2.2 Fluoxastrobin rosickyite azoles (1: 2) compounds the inhibition to sigatoka bacterium mycelial growth
EC50(theoretical value)=1/ [(1/3)/0.0433+ (2/3)/I.9917]=0.1245 μ g/ml
SR50=EC50(theoretical value)/EC50(observed value)=0.1245/0.1137=1.09
5.2.3 Fluoxastrobin rosickyite azoles (1: 4) compounds the inhibition to sigatoka bacterium mycelial growth
EC50(theoretical value)=1/ [(1/5)/0.0433+ (4/5)/1.9917]=0.1992 μ g/ml
SR50=EC50(theoretical value)/EC50(observed value)=0.1992/0.1907=1.04
5.2.4 Fluoxastrobin rosickyite azoles (1: 6) compounds the inhibition to sigatoka bacterium mycelial growth
ECS0(theoretical value)=1/ [(1/7)/0.0433+ (6/7)/1.9917]=0.2681 μ g/ml
SR50=EC50(theoretical value)/EC50(observed value)=0.2681/0.2621=1.02
5.2.5 Fluoxastrobin rosickyite azoles (1: 8) compounds the inhibition to sigatoka bacterium mycelial growth
The Fluoxastrobin rosickyite azoles built agent formulation screening test result of table 3 collects
| Medicament | SR50 | |
| Fluoxastrobin | 0.0433 | / |
| Rosickyite azoles | 1.9917 | / |
| It is phonetic: the third=1: 1 | 0.0872 | 0.97 |
| It is phonetic: the third=1: 2 | 0.1137 | 1.09 |
| It is phonetic: the third=1: 4 | 0.1907 | 1.04 |
| It is phonetic: the third=1: 6 | 0.2621 | 1.02 |
| It is phonetic: the third=1: 8 | 0.3051 | 1.09 |
EC50(theoretical value)=1/ [(1/9)/0.0433+ (8/9)/I.9917]=0.3320 μ g/ml
SR50=EC50(theoretical value)/EC5D(observed value)=0.3320/0.3051=1.09
6. interpretation of result
On AEA culture mediums, Fluoxastrobin suppresses the EC of sigatoka bacterium mycelial growth50Be worth is 0.0433 μ g/ml, rosickyite
The EC of azoles50It is 1.9917 μ g/ml to be worth.After Fluoxastrobin rosickyite azoles is by 1: 1,1: 2,1: 4,1: 6,1: 8 mass ratio compounding,
EC50Value and SR50Value is shown in Table 3, and summation action is mainly shown as between Fluoxastrobin and rosickyite azoles.Comprehensive each side factor, Fluoxastrobin
With rosickyite azoles with 1: 4-1: 8 mass ratio compounding, prevent and treat the better of sigatoka.
The composite bactericide for preventing and treating sigatoka of the invention, after two kinds of base-material mixtures, effect is substantially added, and uses
One of safety, the disease for generally occur for the Banana Growth later stage, influenceing Banana Industry most serious sigatoka bacterium has well
Cytotoxicity, and apparently higher than Fluoxastrobin and rosickyite azoles single dose.It is used to prevent and treat sigatoka suitable for vast rural area.
Specific embodiment
Embodiment 1
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98% Fluoxastrobin active compound 2.0kg, then takes
The mass fraction of biology Co., Ltd of Guizhou road unit production is 98% rosickyite azoles active compound 2.0kg, is re-dubbed 4.0kg preventing and treating Banana Leafs
The composite bactericide of pinta.
Embodiment 2
The mass fraction for taking Zhejiang Heben Science ＆ Technology Co., Ltd.'s production is 98% Fluoxastrobin active compound 2.0kg, then takes Guizhou
The mass fraction of biology Co., Ltd of road unit production is 98% rosickyite azoles active compound 8.0kg, is re-dubbed 10.0kg preventing and treating banana tikka
The composite bactericide of disease.
Embodiment 3
The mass fraction for taking Zhejiang Heben Science ＆ Technology Co., Ltd.'s production is 98% Fluoxastrobin active compound 2.0kg, then takes Guizhou
The mass fraction of biology Co., Ltd of road unit production is 98% rosickyite azoles active compound 16.0kg, is re-dubbed 18.0kg preventing and treating banana tikka
The composite bactericide of disease.
Claims (2)
1. a kind of composite bactericide for preventing and treating sigatoka, it is characterised in that the composite bactericide is with Fluoxastrobin and rosickyite azoles
It is base-material, according to the composite bactericide that the quality proportioning 1: 1~1: 8 of Fluoxastrobin and rosickyite azoles is compounded, the Fluoxastrobin is
The active compound of mass fraction 98%；The rosickyite azoles is 98% active compound.
2. composite bactericide as claimed in claim 1, it is characterised in that the composite bactericide is to be with rosickyite azoles with Fluoxastrobin
Base-material, according to the composite bactericide that the quality proportioning 1: 4~1: 8 of Fluoxastrobin and rosickyite azoles is compounded, the Fluoxastrobin is matter
Measure the active compound of fraction 98%；The rosickyite azoles is 98% active compound.
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| CN201710119427.7A CN106889091A (en) | 2017-03-02 | 2017-03-02 | A kind of composite bactericide for preventing and treating sigatoka |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108849950A (en) * | 2018-08-20 | 2018-11-23 | 贵州道元生物技术有限公司 | A kind of combination sterilization composition for preventing and treating the late blight of potato |
| EP3524052A1 (en) * | 2018-02-09 | 2019-08-14 | Rotam Agrochem International Company Limited | Fungicidal composition and the use thereof in controlling undesired fungal infestations |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102273441A (en) * | 2011-09-13 | 2011-12-14 | 广西田园生化股份有限公司 | Ultralow volume liquid containing azoxystronbin |
| CN104621130A (en) * | 2013-11-15 | 2015-05-20 | 南京华洲药业有限公司 | Sterilization composition containing prothioconazole and azoxystrobin and application thereof |
| CN106212470A (en) * | 2016-07-13 | 2016-12-14 | 南京吉星生物技术开发有限公司 | A kind of bactericidal composition containing Fluoxastrobin and application thereof |
-
2017
- 2017-03-02 CN CN201710119427.7A patent/CN106889091A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102273441A (en) * | 2011-09-13 | 2011-12-14 | 广西田园生化股份有限公司 | Ultralow volume liquid containing azoxystronbin |
| CN104621130A (en) * | 2013-11-15 | 2015-05-20 | 南京华洲药业有限公司 | Sterilization composition containing prothioconazole and azoxystrobin and application thereof |
| CN106212470A (en) * | 2016-07-13 | 2016-12-14 | 南京吉星生物技术开发有限公司 | A kind of bactericidal composition containing Fluoxastrobin and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3524052A1 (en) * | 2018-02-09 | 2019-08-14 | Rotam Agrochem International Company Limited | Fungicidal composition and the use thereof in controlling undesired fungal infestations |
| EP3524052B1 (en) | 2018-02-09 | 2021-03-17 | Rotam Agrochem International Company Limited | The use of a fungicidal mixture to reduce the phytotoxicity of each individual fungicide |
| CN108849950A (en) * | 2018-08-20 | 2018-11-23 | 贵州道元生物技术有限公司 | A kind of combination sterilization composition for preventing and treating the late blight of potato |
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Application publication date: 20170627 |
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