CN109769837A - A kind of pesticide synergistic combination preventing and treating fruits and vegetables gray mold - Google Patents
A kind of pesticide synergistic combination preventing and treating fruits and vegetables gray mold Download PDFInfo
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- CN109769837A CN109769837A CN201910156357.1A CN201910156357A CN109769837A CN 109769837 A CN109769837 A CN 109769837A CN 201910156357 A CN201910156357 A CN 201910156357A CN 109769837 A CN109769837 A CN 109769837A
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- gray mold
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- difenoconazole
- oxazole
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Abstract
The invention discloses a kind of pesticide synergistic combinations for preventing and treating fruits and vegetables gray mold, it is made of oxazole and difenoconazole.The present invention is mixed by by oxazole with difenoconazole being in mass ratio that 1:1 carries out compounding, the combination has apparent synergistic effect to the mycelia growth inhibition of gray mold germ fauna, and for field control effect up to 91% or more, control efficiency is significant, and the dosage of pesticide can be effectively reduced, save cost.The present invention has many advantages, such as the application of efficient, safe, low in cost and pesticide reduction.
Description
Technical field
The present invention relates to plant protection art in agricultural, especially a kind of pesticide synergistic combination for preventing and treating fruits and vegetables gray mold.
Background technique
Wild cabbage gray mold is a kind of fungal disease caused by the pathogen of Botrytis cinerea (Bottytis cinerea), sweet except causing harm
Blue outer, can also cause harm a variety of fruits and vegetables such as tomato, eggplant, Chinese cabbage, radish, grape, often result in a large amount of underproduction.Due to grey grape
Spore bacterium has the characteristics that host range is wide, breeding is fast, hereditary variation is big, grade of fit is high, is also easy to produce drug resistance, to benzo in production
Imidazoles (carbendazim, benomyl), dicarboximide class (procymidone, vinclozolin, iprodione), N- carbanilate
The various sterilizations agent such as class (diethofencarb), anilino-pyrimidine (pyrimethanil, cyprodinil) generally generate resistance.In recent years, no
It is disconnected to there is new type bactericide to be developed and put into control of plant disease, and it is novel there is an urgent need to screen to produce prevention and treatment gray mold
Efficient medicament and its synergistic combination.Therefore, it researchs and develops with conventional pesticide no interactions resistance, with the novel of unique mechanism of action
Fungicide or pharmaceutical agent combinations, to overcome or delay the resistance of Botrytis cinerea and substitute existing conventional pesticide to be in gray mold prevention and treatment
The problem of urgent need to resolve.The present invention filters out the disinfecting for having high virulence to Botrytis cinerea by mycelia growth inhibition assay
Agent and pharmaceutical agent combinations, it is intended to provide technical basis for the prevention and treatment of gray mold and pesticide reduction application.
Summary of the invention
The object of the present invention is to provide it is a kind of prevent and treat fruits and vegetables gray mold pesticide synergistic combination, it have efficiently, safety,
The advantages that environmentally friendly and pesticide reduction application, with overcome the deficiencies in the prior art.
What the present invention was achieved through the following technical solutions:
A kind of pesticide synergistic combination preventing and treating fruits and vegetables gray mold, it is made of oxazole and difenoconazole.
Further, the mass ratio of the oxazole and difenoconazole is 1:1.
Compared with prior art, the present invention the present invention is used to the high interior suction different with mechanism of action of gray mold germ fauna virulence
Property fungicide oxazole compounded with protection and systemic fungicide difenoconazole, control efficiency significantly and reduces pesticide and applies
Dosage.
Specific embodiment
It is further specifically described below by example to of the invention.Involved ingredient or material in following methods, such as without spy
Different explanation is that commercial sources can get.In related experimental methods unless otherwise specified be the existing routine of the art
Method.Numerical value or number ratios therein refer both to mass figures or mass ratio such as without mark.
Embodiment 1:
(1) materials and methods
Strains tested: the pathogen of Botrytis cinerea (Bottytis cinerea), by Guizhou Province, wild cabbage planting base, Xiuwen County wild cabbage
The separation identification of gray mold diseased plant obtains.
Reagent agent: 25.5% oxazole EC (Shenyang Chemical Research Institute), 10% difenoconazole WG (Switzerland's Syngenta
Company).
(2) toxicity test
Indoor virulence test reference " pesticide bioactivity testing standard working specification " carries out, using mycelial growth rate method
Measurement.According to trial test result by reagent agent gradient dilution, drug containing tablet is prepared, each of the above processing is repeated 4 times.With inoculation
Bacteria cake is transferred to each drug containing tablet center by needle, is placed in 28 DEG C of incubators and is cultivated 5d, measures each processing bacterium with crossing method
Fall diameter, calculate inhibiting rate as follows, using DPS carry out data statistic analysis, find out virulence regression equation, related coefficient,
EC50Value.
(3) the mixed co-toxicity evaluation of fungicide
Using mycelial growth rate method, using the mixed principle of the different fungicide of mechanism of action, by two kinds of different sterilizations
Agent in effective component mass ratio be 10:0,9:l, 8:l, 7:1,5:1,3:1,1:1, l:3,1:5,1:7,1:9,0:10 ratio into
Row is mixed, measures its inhibiting rate to the growth of wild cabbage ash arrhizus bacteria, and operation is carried out referring to 1.2.It is calculated according to Sun Yunpei's Method various
The co-toxicity coefficient (CTC) of proportion, and then medicament different ratio is evaluated to the inhibiting effect of Chinese cabbage ash arrhizus bacteria.When CTC is greater than
120, illustrate that two kinds of pharmacy mix have synergistic effect；When CTC is antagonism less than 90；When CTC is added close to 90~120
Effect.The synergistic effect of compounding agent is evaluated according to the size of co-toxicity coefficient, and is taken reagent cost into consideration and determined optimum proportioning.
Mixed agent theoretical toxicity index=Σ (single dose toxicity index × content of the single dose in mixture)
(4) field control effectiveness test
In May, 2016, experimental field it is located at the town Xiuwen County Long Chang wild cabbage planting base, the Vegetable area former years wild cabbage gray mold
It falls ill heavier.Experimental field soil property is loamy soil, and middle fertility is on the upper side.4 processing, 25.5% oxazole EC is arranged in test altogether
225g a.i./ha, 10% difenoconazole WG 60g a.i./ha, 25.5% oxazole EC+10% difenoconazole WG
112.5+30g a.i./ha and clear water control (CK).Experimental plot uses RANDOMIZED BLOCK DESIGN, and 4 repetitions of every cell are small
Area area 30m2, in early stage (May 10), (spray is administered 2 times altogether, is spaced 7d.It is spraying using DX-9A back carried hand
Device spray pesticide, spraying uniform, water consumption 750L/hm2.14d investigates incidence after 2nd application, and calculates disease incidence, prevents
Effect.Calculation method is as follows:
(4) embodiment effect
2 kinds show 25.5% oxazole EC poison to the toxicity test result (table 1) of wild cabbage ash arrhizus bacteria for examination fungicide
Power is most strong, EC50For 0.1356 μ g/mL, next 10% difenoconazole WG (EC50For 5.8642 μ g/mL).
1 fungicide of table is to wild cabbage ash arrhizus bacteria virulence test result
Compounding measurement result shows (table 2) that by 5:1,3:1,1:1 mixing used time, CTC divides for oxazole and difenoconazole
Not Wei 120.34,156.37,223.61, be all larger than 120, synergistic effect is obvious, and 1:1 effect is best.When 9:1,7:1,1:3 are mixed
Used time, CTC are respectively 109.56,115.34,92.46, show as summation action.When 1:5,1:7,1:9 mix the used time, CTC divides
Not Wei 86.13,78.53,84.26, show as antagonism.
2 oxazole of the table co-toxicity to ash arrhizus bacteria mixed with difenoconazole
Field efficacy is the result shows that (table 3), and the single 25.5% oxazole EC and 10% difenoconazole WG of spraying is to sweet
The control efficiency of bluish grey mildew is respectively 85.65% and 67.53%, extremely significant to be lower than compound drug.Spray 25.5% pyridine of mixture
Bacterium oxazole EC+10% difenoconazole WG processing has good synergistic function to the prevention and treatment of wild cabbage gray mold, is above list
Agent processing, preventive effect is up to 91.43%.From table 3, it can also be seen that, oxazole mixed with difenoconazole can significantly improve preventive effect
The usage amount that pesticide can be reduced again has reached good synergistic function and has realized the reducing application amount of pesticide.
The field efficacy of 3 prescription medicament of table prevention and treatment wild cabbage gray mold
Note: significant (P < 0.05) with different lowercase letter indication differences are marked after column data, different capitalizations indicate difference
Extremely significant (P < 0.01).
Certainly, above is specific application example of the invention, and there are other embodiments of the invention, all using equivalent
The technical solution that replacement or equivalent transformation are formed, all falls within protection scope of the presently claimed invention.
Claims (2)
1. a kind of pesticide synergistic combination for preventing and treating fruits and vegetables gray mold, it is characterised in that: it is by oxazole and difenoconazole group
At.
2. the pesticide synergistic combination of prevention and treatment fruits and vegetables gray mold according to claim 1, it is characterised in that: the pyridine bacterium is disliked
The mass ratio of azoles and difenoconazole is 1:1.
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| CN201910156357.1A CN109769837A (en) | 2019-03-01 | 2019-03-01 | A kind of pesticide synergistic combination preventing and treating fruits and vegetables gray mold |
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| CN201910156357.1A CN109769837A (en) | 2019-03-01 | 2019-03-01 | A kind of pesticide synergistic combination preventing and treating fruits and vegetables gray mold |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050797A (en) * | 2019-06-06 | 2019-07-26 | 江西鑫邦科技有限责任公司 | A kind of composition pesticide of brassinolide-containing class compound and oxazole class, triazole bactericidal agent |
| CN112823630A (en) * | 2019-11-21 | 2021-05-21 | 沈阳中化农药化工研发有限公司 | Seed treatment agent for sterilization |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608467A (en) * | 2003-10-24 | 2005-04-27 | 沈阳化工研究院 | Bactericide composition containing SYP-Zo48 |
| CN101087529A (en) * | 2004-12-23 | 2007-12-12 | 巴斯福股份公司 | Fungicidal mixtures |
| WO2014079804A1 (en) * | 2012-11-22 | 2014-05-30 | Basf Se | Pesticidal mixtures |
-
2019
- 2019-03-01 CN CN201910156357.1A patent/CN109769837A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1608467A (en) * | 2003-10-24 | 2005-04-27 | 沈阳化工研究院 | Bactericide composition containing SYP-Zo48 |
| CN101087529A (en) * | 2004-12-23 | 2007-12-12 | 巴斯福股份公司 | Fungicidal mixtures |
| WO2014079804A1 (en) * | 2012-11-22 | 2014-05-30 | Basf Se | Pesticidal mixtures |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110050797A (en) * | 2019-06-06 | 2019-07-26 | 江西鑫邦科技有限责任公司 | A kind of composition pesticide of brassinolide-containing class compound and oxazole class, triazole bactericidal agent |
| CN112823630A (en) * | 2019-11-21 | 2021-05-21 | 沈阳中化农药化工研发有限公司 | Seed treatment agent for sterilization |
| CN112823630B (en) * | 2019-11-21 | 2021-11-23 | 沈阳中化农药化工研发有限公司 | Seed treatment agent for sterilization |
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Application publication date: 20190521 |