CN106719747A - By rosickyite azoles and the bactericide of tricyclazole compounding preventing and treating rice blast - Google Patents
By rosickyite azoles and the bactericide of tricyclazole compounding preventing and treating rice blast Download PDFInfo
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- CN106719747A CN106719747A CN201710119960.3A CN201710119960A CN106719747A CN 106719747 A CN106719747 A CN 106719747A CN 201710119960 A CN201710119960 A CN 201710119960A CN 106719747 A CN106719747 A CN 106719747A
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- Prior art keywords
- tricyclazole
- bactericide
- rice blast
- rosickyite azoles
- azoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The bactericide of the preventing and treating rice blast compounded by the third TBZ and tricyclazole, the bactericide is with rosickyite azoles and tricyclazole as base-material, according to the bactericide that the quality proportioning 1: 15~1: 35 of rosickyite azoles and tricyclazole is compounded；The rosickyite azoles is the active compound of mass fraction 98%, and the tricyclazole is the active compound of mass fraction 95%.Preferred bactericide is, with rosickyite azoles and tricyclazole as base-material, to be formed according to the compounding of quality proportioning 1: 35 of rosickyite azoles and tricyclazole.Two kinds of base-materials of bactericide of the invention do not chemically react, and with obvious synergy or are added effect, using safety, have a good cytotoxicity for current rice blast, and apparently higher than rosickyite azoles or tricyclazole single dose.It is used to prevent and treat rice blast suitable for vast rural area.
Description
Technical field
The present invention is relevant with bactericide, specifically, belongs to the bactericide containing organic compound, furthermore, it is understood that belonging to
Prevent and treat the bactericide of rice blast.The compound drug is in addition to preventing and treating rice blast has special efficacy, moreover it is possible to suppress the spore of Alternaria alternate
The formation of spore is sprouted and adhered to son, so as to prevent the intrusion of germ and subtract oligosporous formation, reaches the effect of prevention.
Technical background
Rice blast (Pyriculariaoryzae) is the great disease during Rice Cropping, in the whole of paddy rice
Can be endangered in growth course.Its distribution is extremely wide, and each main rice region of China is distributed.Can the underproduction 3% as morbidity is latter
~50%, even No kernels or seeds are gathered, as in a year of scarcity for the field of especially severe.Rice blast is by Deuteromycotina fungi Invisible element ash pears spore
Pyricularia grisea (Cooke) Sacc, Perfect stage Magnaporthe grisea Pyricularia oryzae Cav cause.It is raw at present
The hair that rice blast is controlled in comprehensive control is gradually carried out using cultivation between the variety diversity different with genetic background with disease-resistant variety on product
Raw and harm.But because Biological Strains of The Pest is complicated and variability is big, variety resistance is difficult to persistently, therefore each Rice Cropping ground
Area still relies on Agro-chemicals control to control the occurrence degree of disease.
Rosickyite azoles is Albendazole (Albendazole), is a kind of efficient, safety, broad spectrum activity imidazoles series bactericidal agent,
It is disinfectant use in agriculture that China has independent intellectual property right, has significant preventive effect to plurality of plant diseases, and have certain volume increase
Effect；But after using for many years, germ gradually generates the resistance to the action of a drug, causes drug effect to reduce.Tricyclazole (tricyclazole)
It is preventing and treating rice blast bactericide specially, bactericidal action mechanism is mainly the formation for suppressing attachment spore melanin, so as to suppress spore
Sprout and attachment spore is formed, prevent germ from invading people and reduce the generation of magnaporthe grisea spore.
Application preventing and treating rice blast medicine Chinese patent has in recent years：No. 2004100143497《One kind preventing and treating rice blast
The built agent of disease》, No. 2007101868948《A kind of bactericidal composition for preventing and treating rice blast》, No. 2007100215094
《The active bacteria formulation of Brevibacillus brevis TW and preventing and treating rice blast》, No. 200710034388.7《One kind preventing and treating paddy rice rice
The built disinfectant of seasonal febrile diseases, banded sclerotial blight》, No. ZL201010028131.2《Prevent and treat the streptomycete bacterium of rice sheath blight disease and rice blast
Strain》, No. 201010028132.7《A kind of bacterial strain of bacillus for preventing and treating rice blast and banded sclerotial blight》, 2011100704006
Number《Prevent and treat the bacteriocide formula and its application method of rice blast》With No. 201510332882.6《One kind preventing and treating paddy rice rice blast
The compound disinfectant of disease》.The related application part for compounding preventing and treating rice blast with tricyclazole with rosickyite azoles is there is no at present.
The content of the invention
The present invention is intended to provide by rosickyite azoles and the bactericide of tricyclazole compounding preventing and treating rice blast, to slow down pathogen to passing
The system chemical drugs long-term single administration of rosickyite azoles develops immunity to drugs potential risk, strengthens the bactericidal activity of new medicament, improves preventing and treating effect
Fruit and reduction drug cost.
Inventor carries out repeated screening to the agricultural chemicals with killing rice blast pathogen activity, and to the bulk drug of Mixed Pharmacy
Quality proportioning carries out repetition test, and result of the test is contrasted, and finds a kind of compound disinfectant, possess to rice blast pathogen compared with
Strong poisoning ability.
The bactericide of the preventing and treating rice blast compounded by rosickyite azoles and tricyclazole that inventor provides be with rosickyite azoles and
Tricyclazole is base-material, according to the composite bactericide that the quality proportioning 1: 15~1: 35 of rosickyite azoles and tricyclazole is compounded.
Preferably, the bactericide of the preventing and treating rice blast for being compounded by rosickyite azoles and tricyclazole is according to rosickyite azoles and three rings
The composite bactericide of the compounding of quality proportioning 1: 35 of azoles.
Above-mentioned rosickyite azoles is the active compound of mass fraction 98%, is provided by Guizhou Daoyuan Biotechnology Co., Ltd., above-mentioned three
Ring azoles is the active compound of mass fraction 95%, is provided by Jiangsu south pesticide research center.
The screening test that the material of germicide of the above-mentioned preventing and treating rice blast compounded by rosickyite azoles and tricyclazole is matched is such as
Under：
1 test objective
Using indoor living pot-culture method, test rosickyite azoles, tricyclazole single dose are active to the Indoor antibacterial of rice blast fungus,
And design and screen 5 synergy types of proportioning combination, the proportioning now with addition or synergistic effect is sent out, it is that field should
With offer foundation.
2 experimental conditions
2.1 reagent agents are designed with test dose
98% rosickyite azoles active compound is provided by Guizhou Daoyuan Biotechnology Co., Ltd., and 95% Mensurating Tricyclazole Technical is by Jiangsu south
Pesticide research center provides.According to application requirement, 1: 15,1: 20,1: 25,1: 30 and 1: 35 five combination is designed.After experiment
Dose design such as table 1.
The test dose of table 1 is designed
2.2 for examination target
Rice blast (Pyricularia oryae Cav.), indoor Plantlet in vitro and culture.
Rice varieties：Soviet Union drives faint-hearted, rice blast sense kind high, plants in basin, and matrix is peat soil：Vermiculite (3: 1),
Grow to 3 true leaves standby.
3 test methods
3.1 medicaments are prepared
Treatment agent acetone solution, is configured to mother liquor, according to experimental design with 0.1% Tween-80 stepwise dilution.
3.2 chemicals treatments
If only spray clear water is blank.Spraying drives the spraying of 10ml glass using ACO series electromagnetic type air compressors
Device, liquid is uniformly imposed on rice seedlings complete stool, and 3ml liquids are sprayed per basin.Often process 3 repetitions.
3.3 inoculations and culture
Pyricularia oryzae is forwarded to spore is produced in product spore culture medium in advance, and spore is scraped with L-type glass bar, uses two-layer filtered through gauze
Spore suspension；0.05% Tween 80 emulsified water adjusts concentration to lxlO8Individual spore/ml.
After spray 24h, the Fresh spores uniform suspension that will be prepared is inoculated in vacuum side of blade, and inoculation is using ACO series
Electromagnetic type air compressor drives 10ml glass sprayers, per basin 2ml spore suspensions.Postvaccinal rice seedlings are moved in time
In moisture preserving cover, humidified with humidifier timing, prevent spore suspension thousand from falling, shading, temperature control is at 25 DEG C.Rice seedlings after 24h
Move into growth cabinet, 28 DEG C of keeping temperature, RH85%, 30000LUX illumination 16h, dark 8h alternatings.
3.4 adjust Check methods
When blank disease leaf rate is up to more than 50%, Check the 2nd, 3 leaf incidences are adjusted in classification, and grade scale is (with leaf
It is unit, with reference to relevant rice blast grade scale and classification collection of illustrative plates).
0 grade：Disease-free spot；1 grade：There is brown scab；
3 grades：There is typical spindle scab, lesion area accounts for whole leaf area below 5%；
5 grades：Typical scab, lesion area accounts for the 6%~25% of whole leaf area；
7 grades：Typical scab, lesion area accounts for the 26%~50% of whole leaf area；
9 grades：Lesion area accounts for more than the 51% of whole leaf area.
3.5 computational methods
According to Check data are adjusted, the disease for calculating each treatment refers to and prevention effect.
3.6 evaluation methods：Co-toxicity coefficient method
Contents (%) of the toxicity index × A of theoretical toxicity index TTI=A in mixture
Contents (%) of the toxicity index × B of+B in mixture
Co-toxicity coefficient is more than 120, shows there is synergistic effect；Show there is antagonism less than 80；Show have between 80~120
Summation action.
4 result of the tests
The toxicity test of 4.1 single doses
As seen from Table 1, EC of the rosickyite azoles to rice blast fungus50It is not make 90% in 1741.877mg/L, this experiment
Preventive effect, its EC90Value is more than 2000mg/L, EC of the tricyclazole to rice blast fungus5G、EC9(95%CL) value is respectively
1.2284mg/L、17.6383mg/L。
4.25 with the toxicity test for comparing rice blast
The EC of proportioning 1: 25,1: 30 and 1: 3550It is 0.9193~1.1573mg/L to be worth, and activity is higher than two single doses；Proportioning 1
: 15 and 1: 20 EC50Value is respectively 1.6293mg/L and 1.5197mg/L, and activity is between two single doses；Proportioning 1: 15,1:
20 and 1: 30 EC90It is 17.6437~27.1839mg/L to be worth, and activity matches 1: 25 and 1: 35 between two single doses
EC90Value is respectively 13.6504mg/L, 12.8628mg/L, and activity is higher than two single doses.
3 five compoundings of table compare the toxicity test result of rice blast fungus
With comparison rice blast fungus
4.3 synergy evaluations
The albendazole of table 4 matches somebody with somebody the synergy evaluation for comparing rice blast with 5 compoundings of tricyclazole
As seen from Table 4, the co-toxicity coefficient of proportioning 1: 35 is 137.44, more than 120, shows as synergistic effect；Other 4 are matched somebody with somebody
The malicious coefficient of ratio is 80.42~110.39, shows as summation action.
5 conclusions
Rosickyite azoles is effective to rice blast fungus, and tricyclazole has good bacteriostatic activity to rice blast fungus.Both
Have after compounding and be added or synergistic effect.With reference to EC50、EC90, 3 indexs of co-toxicity coefficient, 5 proportionings may be selected, preferred matter
Amount proportioning albendazole: tricyclazole is 1: 35, and the bactericide of compounding has ideal fungistatic effect.
Two kinds of base-materials of bactericide of the preventing and treating rice blast compounded by rosickyite azoles and tricyclazole of the invention do not occur chemical anti-
Should, with obvious synergy or addition effect, using safety, there is good cytotoxicity for current rice blast, and
Apparently higher than rosickyite azoles or tricyclazole single dose.It is used to prevent and treat rice blast suitable for vast rural area.
Specific embodiment
The ︰ of embodiment 1
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 97% rosickyite azoles active compound 1.0kg, then takes
Jiangsu south pesticide research center provide for the bactericide Mensurating Tricyclazole Technical 15.0kg of mass fraction 95% is base-material, be re-dubbed
16.0kg prevents and treats the compound disinfectant of rice blast.
Embodiment 2
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98% rosickyite azoles active compound 1.0kg, then takes
Jiangsu south pesticide research center provide for the bactericide Mensurating Tricyclazole Technical 20.0kg of mass fraction 95% is base-material, be re-dubbed
21.0kg prevents and treats the compound disinfectant of rice blast.
Embodiment 3
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98% rosickyite azoles active compound 1.0kg, then takes
Jiangsu south pesticide research center provide for the bactericide Mensurating Tricyclazole Technical 25.0kg of mass fraction 95% is base-material, be re-dubbed
26.0kg prevents and treats the compound disinfectant of rice blast.
Embodiment 4
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98% rosickyite azoles active compound 1.0kg, then takes
Jiangsu south pesticide research center provide for the bactericide Mensurating Tricyclazole Technical 30.0kg of mass fraction 95% is base-material, be re-dubbed
31.0kg prevents and treats the compound disinfectant of rice blast.
Embodiment 5
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd.'s production is 98% rosickyite azoles active compound 1.0kg, then takes
Jiangsu south pesticide research center provide for the bactericide Mensurating Tricyclazole Technical 35.0kg of mass fraction 95% is base-material, be re-dubbed
36.0kg prevents and treats the compound disinfectant of rice blast.
Above-mentioned compound disinfectant Guizhou somewhere uses, and obtains the good result of preventing and treating rice blast, wherein quality proportioning third
Sulphur imidazoles: tricyclazole be 1: 35 prevention effect it is best.
Claims (2)
1. the bactericide of the preventing and treating rice blast for being compounded by rosickyite azoles and tricyclazole, it is characterised in that the bactericide be with rosickyite azoles with
Tricyclazole is base-material, according to the bactericide that the quality proportioning 1: 15~1: 35 of rosickyite azoles and tricyclazole is compounded；The rosickyite
Azoles is the active compound of mass fraction 98%, and the tricyclazole is the active compound of mass fraction 95%.
2. bactericide as claimed in claim 1, it is characterised in that the bactericide is, with rosickyite azoles and tricyclazole as base-material, to press
According to the bactericide of the compounding of quality proportioning 1: 35 of rosickyite azoles and tricyclazole；The rosickyite azoles is the original of mass fraction 98%
Medicine, above-mentioned tricyclazole is the active compound of mass fraction 95%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710119960.3A CN106719747A (en) | 2017-03-02 | 2017-03-02 | By rosickyite azoles and the bactericide of tricyclazole compounding preventing and treating rice blast |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542648A (en) * | 2015-01-27 | 2015-04-29 | 四川利尔作物科学有限公司 | Sterilization composition and application thereof |
| CN104872155A (en) * | 2015-06-16 | 2015-09-02 | 贵州道元生物技术有限公司 | Compound bactericide capable of preventing and treating rice blast |
| CN105961379A (en) * | 2016-05-04 | 2016-09-28 | 南京高正农用化工有限公司 | Large composition floating granule for preventing and controlling sheath blight and rice blast of rice, and preparation method thereof |
-
2017
- 2017-03-02 CN CN201710119960.3A patent/CN106719747A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542648A (en) * | 2015-01-27 | 2015-04-29 | 四川利尔作物科学有限公司 | Sterilization composition and application thereof |
| CN104872155A (en) * | 2015-06-16 | 2015-09-02 | 贵州道元生物技术有限公司 | Compound bactericide capable of preventing and treating rice blast |
| CN105961379A (en) * | 2016-05-04 | 2016-09-28 | 南京高正农用化工有限公司 | Large composition floating granule for preventing and controlling sheath blight and rice blast of rice, and preparation method thereof |
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