CN115968892B - Insecticidal composition for preventing and controlling taro field red spiders - Google Patents

Insecticidal composition for preventing and controlling taro field red spiders Download PDF

Info

Publication number
CN115968892B
CN115968892B CN202310039526.XA CN202310039526A CN115968892B CN 115968892 B CN115968892 B CN 115968892B CN 202310039526 A CN202310039526 A CN 202310039526A CN 115968892 B CN115968892 B CN 115968892B
Authority
CN
China
Prior art keywords
insecticidal composition
ethionazole
red spiders
taro
osthole
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202310039526.XA
Other languages
Chinese (zh)
Other versions
CN115968892A (en
Inventor
何芳练
董伟清
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Original Assignee
Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences filed Critical Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Priority to CN202310039526.XA priority Critical patent/CN115968892B/en
Publication of CN115968892A publication Critical patent/CN115968892A/en
Application granted granted Critical
Publication of CN115968892B publication Critical patent/CN115968892B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of pesticides, and in particular relates to an insecticidal composition for preventing and controlling taro field red spiders. An insecticidal composition for preventing and controlling taro field red spiders is prepared by binary compounding of etoxazole and osthole. The insecticidal mechanism of the ethionazole and the osthole in the insecticidal composition is different, and the insecticidal composition can act on pests from different action sites when being applied in a compound way, thereby being beneficial to reducing the resistance risk and delaying the generation of drug resistance of the pests. In addition, when the ethionazole and the osthole are compounded in the insecticidal composition, the co-toxicity coefficient of the ethionazole and the osthole for taro field red spiders is higher than 120 in a certain mass ratio range, the insecticidal composition has remarkable synergistic effect, can improve the control effect on the red spiders, is beneficial to reducing the application dosage of pesticides, reduces the pesticide residue and the control cost, and can provide support for developing novel medicaments.

Description

Insecticidal composition for preventing and controlling taro field red spiders
Technical Field
The invention belongs to the technical field of pesticides, and in particular relates to an insecticidal composition for preventing and controlling taro field red spiders.
Background
Taro, also known as taro, taro or taro, is a perennial tuber plant of the genus arisaema, and is an important cash crop. Taro is often damaged by diseases and insects in the whole growth process, so that the quality and the yield of the taro are reduced, and certain economic loss is caused for growers. The taro field main insect pests include prodenia litura, aphid, red spider and the like. Wherein, the red spider is taro Tian Changfa pest, which is in the form of ovum or fertilized female mite in the crack of plant, plant residue and shallow soil layer around root. In the next year, the temperature rises, and overwintering fertilized female mites start to harm the plants which just sprout. The red spiders firstly harm the two sides of the main vein on the back of the leaf to pierce and suck juice, spit silk, net and lay eggs, inhibit photosynthesis of crops, reduce accumulation of nutrients, cause the leaf to turn yellow and lose green, and influence the yield and quality of the crops.
At present, chemical control is still one of the main means for controlling taro field red spiders in production, but due to the fact that a grower uses a single chemical agent for a long time and the like, unreasonable medication causes resistance to a plurality of acaricides to different degrees, and the control effect of the agent is reduced to different degrees. Although the application dosage of the medicament can be increased to a certain extent, the medicament resistance is further increased, and the prevention cost and the pesticide residue are increased. Therefore, research and development of novel agents is necessary.
Cyanotebufenpyrad is used as a lead compound by Shenyang neutralization pesticide chemical industry research and development limited company, and the compound ethaboxam with higher acaricidal activity is discovered by carrying out structural modification on a pyrazole ring and a hydroxyl part in the structure of the compound ethaboxam. The etoxazole is a brand new compound, has no cross resistance with the existing acaricide, has outstanding quick-acting property, can reach extremely high death rate in 1-3 days, has excellent effect on four insect states of red spiders, is safe to crops, and is environment-friendly. However, as the ethaboxam is a chemical agent, although the ethaboxam has better acaricidal activity at present, if the ethaboxam is singly used for a long time, the red spider can slowly accumulate the drug resistance to the ethaboxam, so that the acaricidal activity of the ethaboxam is gradually reduced.
The compounding of different pesticide effective components is a common means for developing new pesticide products and preventing and treating disease and insect pests at present. The formulated medicament will exhibit: the three modes of action of antagonism, additive action and synergistic action, compared with single medicament, the compound medicament with synergistic action can effectively improve the control effect on plant diseases and insect pests and delay the generation of drug resistance of plant diseases and insect pests. The inventor finds that after the ethionazole and the osthole, matrine or cycloxaprid are compounded, the invention has good synergistic effect on taro field red spiders, and can provide support for developing novel medicaments based on the synergistic effect.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide an insecticidal composition for preventing and controlling taro field red spiders, so as to solve the problem of long-term application of a single medicament.
In order to achieve the above purpose, the present invention provides the following technical solutions:
The first aim of the invention is to provide an insecticidal composition for preventing and controlling taro field red spiders, which is formed by binary combination of ethionazole and osthole, matrine or cycloxaprid.
Preferably, the mass ratio of the etoxazole to the osthole is 1-9:9-1.
Preferably, the mass ratio of the etoxazole to the matrine is 1-20:15-1.
Preferably, the mass ratio of the etoxazole to the cycloxaprid is 1-10:19-1.
The second object of the invention is to provide an insecticide comprising the insecticidal composition for controlling taro field red spiders.
Preferably, the mass of the insecticidal composition for preventing and controlling taro field red spiders accounts for 0.75-65% of the total mass of the insecticide, and the balance is auxiliary components which are allowed to be added in the agro-pharmaceutical industry. The pharmaceutically acceptable auxiliary ingredients may be selected from dispersing agents, wetting agents, solvents, fillers, antifreeze agents, preservatives, disintegrants and thickeners, among others, as required by the skilled artisan to process the dosage form.
The third object of the invention is to provide the insecticidal composition for preventing and controlling taro field red spiders or the application of the insecticide in preventing and controlling taro field red spiders.
Compared with the prior art, the invention has the following beneficial effects:
(1) The insecticidal composition has different insecticidal mechanisms of the ethiprole, the osthole, the matrine and the cycloxaprid, and can act on pests from different action sites when being applied in a compound way, thereby being beneficial to reducing the resistance risk and delaying the generation of drug resistance of the pests.
(2) When the ethiprole and the osthole, the matrine or the cycloxaprid in the insecticidal composition are compounded, the co-toxicity coefficient of the ethiprole to the taro red spiders is higher than 120 in a certain mass ratio range, the insecticidal composition has remarkable synergistic effect, can improve the control effect to the red spiders, is beneficial to reducing the application dosage of pesticides, reduces the pesticide residue and the control cost, and can provide support for developing novel medicaments.
Detailed Description
The invention will be better understood from the following examples, which are described only for illustration of the invention and should not be construed as limiting the invention as detailed in the claims.
Examples: indoor biological Activity assay
1. Test object: red spiders are collected from taro planting areas in Guangxi Lipu, and are fed with fresh taro leaves in a laboratory of the national institute of agricultural sciences of Guangxi Zhuang nationality, and healthy and active taro field red spiders female mites are selected as test objects.
2. Test agent: 98% of ethionazole nitrile crude drug (Shenyang scientific chemical Co., ltd.), 98% of osthole crude drug (Shanghai Yuan Ye Biotechnology Co., ltd.), 98% of matrine crude drug (Shanghai Yuan Ye Biotechnology Co., ltd.), 97% of cycloxaprid crude drug (Liaoning Zhonghui Biotechnology Co., ltd.).
3. Test method (refer to NT/T1154.13-2008 pesticide laboratory test rules pesticide section 13: leaf disk spray method)
3.1 Preparation of medicament: the preparation method comprises the steps of dissolving the tested medicament with dimethyl sulfoxide to prepare a single-dose mother solution, diluting with 0.1% Tween-80 aqueous solution, setting a plurality of groups of proportions, and setting 7 gradient mass concentrations of each single dose and each group of proportions according to an equal ratio method for standby.
3.2 Pest treatment: selecting clean taro leaves with consistent growth, and making the leaves into leaf discs by using a puncher with the diameter of 2 cm. Placing a wet sponge block in a culture dish with the diameter of 9cm, placing filter paper on the wet sponge block, placing leaf discs on the filter paper, and placing 2 leaf discs on each dish; the test subjects were inoculated onto leaf discs, each leaf disc inoculated with 20 heads. The dishes were sprayed on a Potter spray tower chassis at a spray pressure of 1.47X 10 5 Pa, with a spray volume of 1mL, each treatment set up for 4 replicates, and a treatment with an aqueous solution containing only 0.1% Tween-80 was set up as a blank. The liquid medicine is taken out after sinking for 1min, the top of the culture dish is covered by 100 mesh gauze and is transferred to the temperature (25+/-1) DEG C and the photoperiod L: and D= (16:8) h, and continuing feeding.
3.3 Investigation and calculation: after the treatment, the death of the test subjects was observed 48 hours (the death was determined by touching the appendages of the mites with a soft writing brush, and the total number of insects and the dead number of the mites were recorded for each treatment, and the corrected mortality of each treatment was calculated from the total number of insects and the dead number of the insects.
In the above formula: p- -mortality in units of; k- -number of dead insects; n- -total number of insects treated.
In the above formula: p 1 -correct mortality in units of; p t - -mortality in units of treatment; p 0 - -control mortality in%.
4. Data analysis: regression analysis was performed on the log values of the concentrations of each treatment agent and the corrected mortality rate values of each treatment using DPS software, LC 50 for each treatment agent was calculated, and co-toxicity coefficients (CTC values) for the blends were calculated according to the grand cloud Pei method.
In the above formula: ati—the measured virulence index of the mixture; s- -LC 50 of standard agent in mg/L; m- -LC 50 of the mixture in mg/L.
TTI=TIA×PA+TIB×PB
In the above formula: TTI- -theoretical toxicological index of the mixture; toxicity index of TI A - -A agents; the percentage content of the P A -A medicament in the mixture is expressed as percentage (%); toxicity index of TI B - -B agent; the percentage of the P B -B medicament in the mixture is expressed as percentage (%).
In the above formula: ctc—co-toxicity coefficient; ati—actual measured virulence index of the mixture; tti—the theoretical toxicity index of the mixture.
5. Evaluation of drug efficacy
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-3.
TABLE 1 toxicity measurement results of ethionazole and acarbose compounded with ostomate red spiders
Medicament name and ratio LC50(mg/L) ATI TTI CTC
Etoxazole mite nitrile 1.02 100.00 -- --
Osthole 17.15 5.95 -- --
Ethionazole mite nitrile 1: osthole 9 3.58 28.49 15.35 185.58
Ethionazole mite nitrile 1: osthole 5 2.72 37.50 21.62 173.43
Ethionazole mite nitrile 1: osthole 3 1.33 76.69 29.46 260.32
Ethionazole mite nitrile 1: osthole 1 1.24 82.26 52.97 155.28
Ethionazole acarbose 3: osthole 1 0.91 112.09 76.49 146.55
Ethionazole acarbose 5: osthole 1 0.66 154.55 84.32 183.27
Ethionazole acarbose 9: osthole 1 0.70 145.71 90.59 160.84
As can be seen from Table 1, at 1-9: within the mass ratio of 9-1, the co-toxicity coefficient of the ethionazole and the osthole to the taro field red spiders after the combination is more than 120, which shows a synergistic effect.
TABLE 2 toxicity measurement results of ethionazole and matrine compounded for taro field red spider
Medicament name and ratio LC50(mg/L) ATI TTI CTC
Etoxazole mite nitrile 1.02 100.00 -- --
Matrine 34.59 2.95 -- --
Ethionazole mite nitrile 1: matrine 15 7.25 14.07 9.01 156.07
Ethionazole mite nitrile 1: matrine 10 4.60 22.17 11.77 188.37
Ethionazole mite nitrile 1: matrine 5 3.16 32.28 19.12 168.78
Ethionazole mite nitrile 1: matrine 3 2.73 37.36 27.21 137.30
Ethionazole mite nitrile 1: matrine 1 1.54 66.23 51.47 128.67
Ethionazole acarbose 3: matrine 1 0.96 106.25 75.74 140.29
Ethionazole acarbose 5: matrine 1 0.53 192.45 83.82 229.59
Ethionazole mite nitrile 10: matrine 1 0.36 283.33 91.18 310.75
Ethionazole mite 15: matrine 1 0.24 425.00 93.93 452.44
Ethionazole 20: matrine 1 0.40 255.00 95.38 267.36
As can be seen from Table 2, at 1-20: within the mass ratio of 15-1, the co-toxicity coefficient of the ethionazole and the matrine to the taro field red spider after being compounded is more than 120, and the synergy is shown. In particular, the mass ratio is 15:1, the co-toxicity coefficient reaches 452.44, and the synergy is most remarkable.
TABLE 3 toxicity measurement results of ethionazole and cycloxaprid compounded for taro field red spiders
As can be seen from Table 3, at 1-10: within the mass ratio of 19-1, the co-toxicity coefficient of the ethionazole and the cycloxaprid to the taro field red spiders after being compounded is more than 120, and the synergistic effect is shown. In particular, the mass ratio is 1:5, the co-toxicity coefficient reaches 602.96, and the synergy is most remarkable.
In conclusion, when the ethionazole is compounded with the osthole, the matrine or the cycloxaprid, the synergistic effect is shown in a certain mass ratio range, the control effect on taro field red spiders can be improved, the application dosage of the chemical agent can be reduced based on the synergistic effect, and the problems of pesticide residue, environmental pollution and the like caused by large-dose application are avoided to a certain extent.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims (4)

1. The insecticidal composition for preventing and controlling taro field red spiders is characterized by being formed by binary compounding of ethionazole and osthole, wherein the mass ratio of the ethionazole to the osthole is 1-9:9-1.
2. An insecticide comprising the insecticidal composition for controlling taro field red spiders of claim 1.
3. The pesticide according to claim 2, wherein the mass of the insecticidal composition for controlling taro field red spiders accounts for 0.75-65% of the total mass of the pesticide, and the balance is auxiliary components which are allowed to be added in the agro-pharmaceutical industry.
4. Use of the insecticidal composition for controlling taro field red spiders of claim 1 or the insecticide of claim 3 for controlling taro field red spiders.
CN202310039526.XA 2023-01-13 2023-01-13 Insecticidal composition for preventing and controlling taro field red spiders Active CN115968892B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310039526.XA CN115968892B (en) 2023-01-13 2023-01-13 Insecticidal composition for preventing and controlling taro field red spiders

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310039526.XA CN115968892B (en) 2023-01-13 2023-01-13 Insecticidal composition for preventing and controlling taro field red spiders

Publications (2)

Publication Number Publication Date
CN115968892A CN115968892A (en) 2023-04-18
CN115968892B true CN115968892B (en) 2024-05-03

Family

ID=85966568

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310039526.XA Active CN115968892B (en) 2023-01-13 2023-01-13 Insecticidal composition for preventing and controlling taro field red spiders

Country Status (1)

Country Link
CN (1) CN115968892B (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104663670A (en) * 2013-11-26 2015-06-03 中国中化股份有限公司 Synergistic miticidal composition
CN111345304A (en) * 2020-04-08 2020-06-30 广西壮族自治区亚热带作物研究所(广西亚热带农产品加工研究所) Insecticidal composition for preventing and treating red spiders of macadamia ternifolia
WO2022166842A1 (en) * 2021-02-07 2022-08-11 山东康乔生物科技有限公司 Composition comprising benzylamine acaricide and use thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104663670A (en) * 2013-11-26 2015-06-03 中国中化股份有限公司 Synergistic miticidal composition
CN111345304A (en) * 2020-04-08 2020-06-30 广西壮族自治区亚热带作物研究所(广西亚热带农产品加工研究所) Insecticidal composition for preventing and treating red spiders of macadamia ternifolia
WO2022166842A1 (en) * 2021-02-07 2022-08-11 山东康乔生物科技有限公司 Composition comprising benzylamine acaricide and use thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
江苏省句容市草莓农药减施增效技术模式;吉沐祥 等;江苏农业科学;第49卷(第15期);第107-112页 *

Also Published As

Publication number Publication date
CN115968892A (en) 2023-04-18

Similar Documents

Publication Publication Date Title
CN114916555B (en) Application of pesticide composition containing bistrifluron in controlling plant pest mites
CN115968892B (en) Insecticidal composition for preventing and controlling taro field red spiders
CN115211427B (en) Insecticidal composition containing mesoionic insecticide and application thereof
CN116076516A (en) Mite-killing composition and application thereof
CN116210712A (en) Insecticidal composition containing diafenthiuron and application thereof
CN109699649B (en) Acaricidal composition containing cyflumetofen
CN114287450A (en) Pesticide composition for preventing and treating sugarcane thrips
CN111345304A (en) Insecticidal composition for preventing and treating red spiders of macadamia ternifolia
CN113632797B (en) Synergistic composition for preventing and treating papaya mites
CN115474603B (en) Insecticidal composition for preventing and controlling bemisia tabaci in taro field
CN104488915A (en) insecticidal composition containing IPP
CN113729028B (en) Pesticide for preventing and treating sugarcane thrips
CN115568468B (en) Synergistic composition containing eugenol for controlling papaya aphids
CN114586789B (en) Mite-killing composition and application thereof
CN115251079B (en) Composition for preventing and controlling taro field aphids
CN116267954A (en) Insecticidal and acaricidal composition for preventing and controlling cassava red spiders
CN116019116B (en) Pesticide powder and application method thereof
CN115299446B (en) Nanometer suspending agent composition containing chlorfenapyr
CN115005226A (en) Insecticidal composition for preventing and treating corn armyworm
CN109122692B (en) Insecticidal and acaricidal pesticide composition with synergistic effect
CN115005224A (en) Biopesticide for preventing and treating tetranychus urticae koch
CN105248446A (en) Complex synergism insecticide and application thereof
CN116548460A (en) Application of dinotefuran-containing composition in preventing and controlling cauliflower cabbage caterpillars
CN104872172B (en) A kind of Recompounded pesticide of preventing and treating green plant bug and preparation method thereof
CN116473067A (en) Pesticide composition for preventing and controlling Tetranychus cinnabarinus

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant