CN109122692B - Insecticidal and acaricidal pesticide composition with synergistic effect - Google Patents

Abstract

The invention discloses an insecticidal and acaricidal pesticide composition with synergistic effect and its application. The insecticidal and acaricidal composition of the present invention comprises the active ingredients fluoropyranone and oxafen, and the weight ratio of the fluoropyranone and oxafen is 50:1～1:50, preferably 20:1～ 1:20. The two groups of active ingredients of the present invention have a significant synergistic effect on various pests and mites such as Tetranychus two-spotted mites, Bemisia tabaci, and brown planthoppers on crops after being compounded, and the control effect is obviously better than that of a single agent alone. The composition has the advantages of expanding the insecticidal spectrum, prolonging the pest control time, delaying the development of resistance and the like, and will have a good application prospect.

Description

A kind of insecticidal and acaricidal pesticide composition with synergistic effect

1. Technical field

The invention belongs to the technical field of agricultural harmful biochemical prevention and control, and in particular relates to an insecticidal and acaricidal composition comprising fluoropyranone and acetazolpyr as active ingredients, which is suitable for preventing and treating two-spotted spider mites, Bemisia tabaci, N. lugens and the like. Agricultural pests.

2. Background technology

Flupyradifurone is a new crotonic acid lactone insecticide developed by Bayer. It acts on the central nervous system of insects and is an antagonist of insect nicotinic acetylcholine receptors. The insecticide has systemic absorption, contact killing, stomach poisoning and osmotic effects, and can be used to control piercing-sucking pests, file-sucking pests and Diptera larvae on vegetables, fruit trees, rice, row crops and ornamental plants Such as whiteflies, psyllids, scale insects, leafhoppers, planthoppers, thrips and Liriomyza sativa, etc., and reduce the spread of viruses and bacteria by insect vectors. It is a fast, efficient, lasting effect with low toxicity environmentally friendly pesticides.

Ethoxafen is a new type of acaricide with independent intellectual property rights in my country. It is metabolized and converted into hydroxyl compounds in mites, inhibiting the effect of succinate dehydrogenase, and then acting on complex II in the respiratory electron transport chain, destroying energy synthesis. , to achieve preventive effect. The insecticide has both stomach poisoning and contact killing effects, and exhibits extremely high activity against herbivorous mites such as citrus panonychus, citrus embroidered mites, apple spider mites, cotton spider mites, and eggplant spider mites. Period has a better control effect. Ethoxafen not only has a new structure, has no cross-resistance with existing acaricides, and has good quick-acting properties, but also is safe and environmentally friendly to crops, has almost no impact on beneficial organisms, and has no biological enrichment.

At present, chemical control is still the most common and effective means of plant pest control, but long-term continuous high-dose application of a single chemical pesticide is not only easy to cause pests to develop resistance, but also easy to cause a series of problems such as pesticide residues and environmental pollution. However, the pace of development of new agents is far outpaced by the rate at which pest resistance develops. Therefore, rational chemical pesticide compounding or mixing can expand the insecticidal spectrum, improve the control effect, prolong the application period, reduce the dosage, reduce the phytotoxicity, reduce the residue, and delay the occurrence of pest resistance and drug resistance. With the positive characteristics of development, the compounding or compounding of pesticides is one of the most effective methods to solve the above problems.

3. Content of the Invention

The purpose of the present invention is to provide an insecticidal and acaricidal composition containing fluoropyranone and acetafenone, the composition has obvious synergistic effect, high insecticidal and acaricidal activity, low toxicity and environmental protection. It can be used to prevent and control two-spotted spider mites, whiteflies, planthoppers and other pests in agriculture.

4. Technical solutions

The technical solution of the present invention is achieved through the following measures:

A kind of insecticidal and acaricidal composition containing fluoropyranone and oxapyr, the active ingredients are fluoropyranone and oxafen, and the weight ratio of fluoropyranone and oxafen is 1:50～ 50:1, the preferred ratio is 1:20～20:1, and the total weight percentage of fluoropyranone and tolafenamide in the composition is 0.5～90%, preferably 10～70%, and the rest are in the pesticide. Permitted and acceptable auxiliary ingredients.

The auxiliary components are solvent, emulsifier, wetting agent, dispersant, stabilizer, antifreeze, thickener, binder, disintegrant, defoamer, penetrant, polymer capsule wall material, thickener Efficacy agents, preservatives and fillers are all commonly used or permitted ingredients in pesticide formulations, and are not particularly limited.

The pesticidal composition of the present invention can be prepared into various dosage forms suitable for agricultural use, including but not limited to suspensions, aqueous emulsions, microcapsule suspensions, wettable powders, water-dispersible granules, microemulsions and the like. The type of dosage form to be selected and processed in specific use can be actually determined according to different objects of use, use environments, and the like.

Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. Compared with a single agent, the composition has obvious synergistic effect, which improves the control effect; 2. The composition can comprehensively control the The piercing-sucking pests and mites have a wide control spectrum and good control effect; 3. The composition is composed of two active ingredients with different mechanisms of action, which is conducive to overcoming and delaying the generation of drug resistance; 4. The compounding of drugs reduces the dosage , thereby reducing costs and reducing environmental pollution.

Five, the specific implementation

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention is described with the following specific embodiments, but the present invention is not limited thereto. The percentages in the examples are all weight percentages.

(1) Indoor bioassay compound screening test

According to the Sun Yunpei method, the co-toxicity coefficient (CTC value) of the mixture was calculated. According to the NY/T11547.7-2006 pesticide combination action classification standard, the synergistic effect of the mixed use of pesticides was evaluated: the co-toxicity coefficient (CTC) ≥ 120 indicated a synergistic effect; the co-toxicity coefficient (CTC) ≤ 80 indicated an antagonistic effect; 80 <Co-toxicity coefficient (CTC)<120 showed additive effect.

Measured virulence index (ATI)=(standard drug LC ₅₀ /test drug LC ₅₀ )×100.

Theoretical toxicity index (TTI)=toxicity index of agent A×percentage content of A in the mixture+toxicity index of agent B×percentage content of B in the mixture.

Co-toxicity coefficient (CTC)=[measured toxicity index (ATI) of mixture/theoretical toxicity index (TTI) of mixture]×100.

The calculation formulas of insect population decline rate and corrected control effect are as follows:

Example 1: Indoor combined virulence determination of flupyridone and ethifenil against Tetranychus bisporus

Subject: Two-spotted spider mite Tetranychus urticae

Test method: use the glass slide dipping method. Affix two tapes with a width of one centimeter on one end of the glass slide, select female nymphs of the same size, and use a brush to gently stick the back of the insects on the tape, stick 30 heads on each piece, and place them on the double mites for 3-4 hours. Examination under the eye dissecting microscope to exclude abnormal and dead individuals. Immerse the end of the glass slide with the test mites in the liquid for 5s, and then quickly use a filter paper strip to dry up the excess liquid around the worms. At 27±1℃, the number of dead and living mites was examined by microscope after 24 hours (touch their feet with a fine brush, and those who did not respond were considered dead).

Table 1 Determination of indoor combined toxicity of flupyridone and acetafenone against Tetranychus 2-spotted mites

Drug name and ratio (weight ratio) LC₅₀(mg/L) ATI TTI CTC Fluoropyranone 3.24 100 - - Acetaxel 1.77 183.05 - - Fluoropyranolone:Ethoxafen (50:1) 2.29 141.48 101.63 139.22 Fluoropyranolone:Ethoxafen (20:1) 1.62 200.00 103.95 192.39 Fluoropyranone:Ethoxafen (10:1) 1.45 223.45 107.55 207.76 Fluoropyranone:Ethoxafen (5:1) 1.3 249.23 113.84 218.93 Fluoropyranone:Ethoxafen (2:1) 1.16 279.31 127.68 218.75 Fluoropyranone:Ethoxafen (1:1) 0.95 341.05 141.53 240.98 Fluoropyranone:Ethoxafen (1:2) 0.91 356.04 155.37 229.16 Fluoropyranolone:Ethoxafen (1:5) 1.05 308.57 169.21 182.36 Fluoropyranone:Ethoxafen (1:10) 1.14 284.21 175.50 161.94 Fluoropyranone:Ethoxafen (1:20) 1.21 267.77 179.10 149.51 Fluoropyranone:Ethoxafen (1:50) 1.29 251.16 181.42 138.44

It can be seen from Table 1 that the co-toxicity coefficient of the compound composition of fluoropyranone and acetazolidine in the range of 50: 1 to 1: 50 for the control of Tetranychus two-spotted mites is above 120, showing a synergistic effect. Especially when the ratio of the two is 1:10 to 10:1, the co-toxicity coefficients for the control of Tetranychus two-spotted mites are all above 160, and the synergistic effect is more obvious.

Example 2: Indoor combined virulence assay of flupyridone and etazol to Bemisia tabaci

Subject: Bemisia tabaci

Test method: dipping leaf agar method. Cut off the round bottom end of the 100mL round-bottom centrifuge tube to make a bioassay tube, put 1.5mL of 1% agar in the cap of the centrifuge tube on one side of the bioassay tube, and put the cotton leaves punched with a hole punch in different concentrations. Soak for 10 s in the liquid or deionized water containing 0.05% Triton X-100 (control), dry it and place it on the agar lightly with the back facing up. After 20-30 2-day-old Bemisia tabaci adults were inserted into each tube, they were sealed with gauze, and the open end of the biometric tube was placed down in an artificial climate room for 1 hour, and the wounds that fell off the straight tube were checked and removed. and dead individuals. 48h after the treatment, the death of the test insects was checked, the total number of insects and the number of dead insects were recorded, and the reduction rate of the insect population was calculated. The death judgment standard is: touch the body of the worm completely with a small brush or move the foot slightly and record it as a dead worm.

Table 2 Indoor combined virulence determination of flupyridone and acetafenone against Bemisia tabaci

As can be seen from Table 2, the co-toxicity coefficient of the compound composition of flupyridone and acetafenpyr in the range of 50: 1 to 1: 50 for the control of Bemisia tabaci is more than 120, showing a synergistic effect; Especially when the ratio of the two is 1:10-10:1, the co-toxicity coefficients for the control of Tetranychus two-spotted mites are all above 160, and the synergistic effect is more obvious.

Example 3: Indoor combined virulence assay of flupyridone and acetazolidine against N. lugens

Subject: Nilaparvata lugens

Test method: pick rice stem dipping method. Select the robust and consistent tillering stage rice seedlings cultivated in the laboratory, dig out the roots, wash them, cut them into 10 cm long rice stems with roots, and dry them in a cool place until there are no water marks on the surface. Soak the rice stems in the diluted medicament for 30s, take them out to dry, hold the roots with soaked absorbent cotton and put them into a glass tube, take the 3rd instar nymphs and put them in, 20 heads per cup, and take the soaked water as the blank. For controls, each concentration was repeated 4 times. After treatment, the animals were reared in a light incubator with a temperature of 27±1°C and a photoperiod of 16h:8h (L:D). Deaths were checked after 4 days.

Table 3 Indoor combined virulence determination of flupyridone and acetazolidine against N. lugens

It can be seen from Table 3 that the co-toxicity coefficients of the compound composition of flupyridone and acetazolamide in the range of 50: 1 to 1: 50 for the control of N. lugens are all above 120, showing a synergistic effect; especially When the ratio of the two is 1:10-10:1, the co-toxicity coefficient of the two-spotted spider mite control is above 160, and the synergistic effect is more obvious.

(2) Formulation Examples

Example 4: 20% Fluoropyranone·Ethoxafen suspending agent

Fluoropyranone 10%, Acetaxel 10%, Sodium Alkyl Naphthalene Formaldehyde Condensate Sulfonate 4%, Calcium Alkyl Sulfonate 5%, Methyl Cellulose 4%, Ethylene Glycol 5%, Silicone Oil 1.5% , deionized water to make up 100%, to obtain 20% fluoropyranone·etoxafen suspending agent.

(3) Example of field efficacy

In order to better verify the practical application effect of the present invention, field efficacy tests of Tetranychus two-spotted mites, Bemisia tabaci and N. lugens were carried out using the preparation of Example 4 of the present invention.

Example 5: Field efficacy test on the control of Tetranychus two-spotted mites with 20% fluoropyranone·etoxafen suspension

The test method refers to "Pesticide Field Efficacy Test Guidelines (1) Acaricides for the Control of Tetranychus and Vegetables GB/T17980.17-2000". 20 leaves were fixed in each plot, and the number of live and nymph mites was counted. Each treatment was replicated 4 times in random block arrangement.

Test reagents: (1) 20% fluoropyranone·etoxafen suspending agent 4000 times; (2) 20% fluoropyranone·etoxafen suspending agent 2000 times; (3) 20% fluoropyran 1000 times of ketone·etoxafen suspending agent; (4) 1500 times of 17% fluoropyranone solution; (5) 3000 times of 30% acetafenone suspending agent; (6) clear water.

Investigate the number of insect populations and calculate the control effect on 3 days, 7 days and 14 days after the drug, and the test results are shown in Table 4.

Table 4 Field efficacy test of 20% fluoropyranone·etoxafen suspension for control of Tetranychus two-spotted

As can be seen from Table 4, the mixed medicaments of different proportions were tested in the field according to different dosages. The acaricidal effect increases with the dosage, and the control period is long. There is no adverse effect on eggplant within the scope of the test drug, and it has application value.

Example 6: Field efficacy test of 20% fluoropyranone·etoxafen suspension for preventing and controlling Bemisia tabaci

The test method refers to the "People's Republic of China Industry Standard NY/T 1464.43-2012: Pesticide Control of Vegetable Bemisia tabaci". 10 tomato plants were fixed in each plot, the upper 3 leaves were taken, and the number of live adults was counted. Each treatment was replicated 4 times in random block arrangement.

Test reagents: (1) 20% fluoropyranone·etoxafen suspending agent 8000 times; (2) 20% fluoropyranone·etoxafen suspending agent 4000 times; (3) 20% fluoropyran 2,000 times of ketone·etoxafen suspending agent; (4) 4,000 times of 17% fluoropyranone soluble agent; (5) 2,000 times of 30% oxafen suspending agent; (6) clear water.

The number of insect populations was investigated on 3 days, 7 days and 14 days after the treatment and the control effect was calculated. The test results are shown in Table 5.

Table 5 Field efficacy test of 20% fluoropyranone·etoxafen suspension against Bemisia tabaci

As can be seen from Table 5, the mixed medicaments of different proportions are carried out field tests according to different dosages, and the control effect on tomato whitefly after the medicine is all better than the control medicament, and the killing effect of fluoropyranone and acetafenone The insect effect increases with the dosage, and the control period is long. There was no adverse effect on tomato within the scope of the test drug.

Example 7: Field efficacy test of 20% Fluoropyranone·Ethoxafen suspension for controlling brown planthopper

The test method refers to the "Pesticide Field Efficacy Test Guidelines (1) Pesticide Control of Rice Planthopper GB/T 17980.4-2000". 10 points were investigated by the parallel hopping method per cell, with 2 clusters per point. Shake or beat the rice bushes, and count the number of planthoppers floating on the water between the rice bushes. Each treatment was replicated 4 times in random block arrangement.

Test reagents: (1) 20% fluoropyranone·etoxafen suspending agent 8000 times; (2) 20% fluoropyranone·etoxafen suspending agent 4000 times; (3) 20% fluoropyran 2,000 times of ketone·etoxafen suspending agent; (4) 4,000 times of 17% fluoropyranone soluble agent; (5) 2,000 times of 30% oxafen suspending agent; (6) clear water.

Investigate the number of insect populations and calculate the control effect on 3 days, 7 days and 14 days after the drug, and the test results are shown in Table 6.

Table 6 Field efficacy test of 20% fluoropyranone·etoxafen suspension for controlling N. lugens

As can be seen from Table 6, the results of the field efficacy test show that the combination of fluoropyranone and acetazolidine can effectively prevent and control the rice brown planthopper, the composition of the present invention has obvious synergistic effect, and the control effect is better than that of the single agent , and the control effect period is long; it has no adverse effect on rice within the scope of the experimental drug, and has application value.

Claims (2)

1. The insecticidal and acaricidal composition is characterized by comprising effective components of fluoropyrafuranone and ethiprole, wherein the weight ratio of the effective components of the fluoropyrafuranone to the ethiprole is 50: 1-1: 50.

2. An insecticidal and acaricidal composition according to claim 1, which is useful against Tetranychus urticae, Bemisia tabaci and Nilaparvata lugens.