CN110973135B

CN110973135B - Compound pesticide of nicotine and allicin and application thereof in preventing and controlling myzus persicae

Info

Publication number: CN110973135B
Application number: CN201911240756.2A
Authority: CN
Inventors: 苏贤坤; 曹毅; 许冬青; 张婕; 朱文静; 巴金莎; 陈兴江; 贾蒙骜; 夏范讲; 宋光龙; 刘国权; 马骏; 高贵
Original assignee: Guizhou Institute of Tobacco Science
Current assignee: Guizhou Institute of Tobacco Science
Priority date: 2019-12-06
Filing date: 2019-12-06
Publication date: 2022-01-11
Anticipated expiration: 2039-12-06
Also published as: CN110973135A

Abstract

The invention discloses a compound pesticide of nicotine and allicin, which is prepared by mixing and compounding nicotine and allicin, wherein the concentration of the nicotine is more than or equal to 0.30g/kg, the concentration of the allicin is more than or equal to 0.60g/kg, and the weight ratio of the nicotine to the allicin is 50: 100. according to the invention, after the nicotine and the allicin are compounded according to a certain proportion, the killing effect of the compound preparation on the myzus persicae is measured under the laboratory condition, and the result shows that the compound preparation of the nicotine and the allicin can obviously improve the aphid killing effect compared with a single preparation. The invention also discloses application of the compound pesticide of nicotine and allicin in preventing and treating myzus persicae.

Description

Compound pesticide of nicotine and allicin and application thereof in preventing and controlling myzus persicae

Technical Field

The invention relates to a compound pesticide of nicotine and allicin and application thereof in preventing and controlling myzus persicae, belonging to the technical field of myzus persicae disinsection.

Background

Myzus persicae (Sulzer) hosts are wide, are one of main pests on tobacco plants, and are distributed in tobacco regions of China. Myzus persicae affects the growth of tobacco plants and induces soot diseases, and more seriously, myzus persicae spreads virus diseases, thus causing great loss to tobacco production. The most common method of application is the application of chemical insecticides, which are resistant to the development of aphid resistance by repeated, long-term use. In order to effectively control myzus persicae, the usage amount of the insecticide is continuously increased, the resistance of myzus persicae is continuously enhanced, and the problems of pesticide residue in products and environmental pollution are caused.

However, the long-term, large, and unreasonable use of chemical pesticides, as well as the inherent disadvantages of certain agents, have caused the problem of "3R" development by pests. Therefore, in order to find bioactive substances and research and develop novel biopesticides, attention is paid at home and abroad, and among them, plant-derived pesticides are the most spotlighted.

Nicotine is an important secondary metabolite of tobacco, and plays a role in killing insects by acting on the nervous system of insects and disturbing the transmission of nerve impulses of the insects. Allicin is a kind of compound extracted from garlic, and has the functions of repelling aphid, contact killing, inhibiting population and fumigating, and nicotine has the functions of contact killing and stomach toxicity, and their action mechanisms are different. Nicotine and allicin both belong to biological pesticides, and with the high concern of people on the safety problem of agricultural products, two substances have wide application prospects in the aspect of pest control, but the biological pesticide has the defects of slow effect taking and poor control effect compared with a chemical pesticide, so that the popularization of the two pesticides is limited to a certain extent.

At present, no technical scheme for the combined use of nicotine and allicin is reported.

Disclosure of Invention

The invention aims to provide a compound pesticide of nicotine and allicin, which has a synergistic effect and good control effect.

The technical scheme of the invention is as follows: a compound pesticide of nicotine and allicin is prepared by mixing and compounding nicotine and allicin.

Preferably, the concentration of nicotine is more than or equal to 0.30g/kg, and the concentration of allicin is more than or equal to 0.60 g/kg.

Preferably, the weight ratio of nicotine to allicin is 50: 100.

preferably, the compound pesticide is a suspending agent, wettable powder, water dispersible granules or microemulsion.

The invention also provides application of the compound pesticide of nicotine and allicin in preventing and treating myzus persicae.

The invention has the beneficial effects that: according to the invention, after the nicotine and the allicin are compounded according to a certain proportion, the killing effect of the compound preparation on the myzus persicae is measured under the laboratory condition, and the result shows that the compound preparation of the nicotine and the allicin can obviously improve the aphid killing effect compared with a single preparation.

Detailed Description

The present invention will be described in detail with reference to the following embodiments in order to make the aforementioned objects, features and advantages of the invention more comprehensible. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.

The invention relates to a compound pesticide of nicotine and allicin, which is prepared by mixing and compounding nicotine and allicin.

Preferably, the weight ratio of nicotine to allicin is 50: 100.

in practical application, the compound pesticide can be prepared into various forms such as suspending agents, wettable powder, water dispersible granules, micro-emulsions and the like.

Examples

Nicotine and allicin in different weight ratios are selected and added into water to prepare compound insecticides with different concentrations, which are shown in table 1 specifically, and the test effect is examined.

Comparative example

In accordance with the examples, a single dose of nicotine or allicin was added to water to produce different concentrations of insecticide, as shown in table 1, and the test results were examined.

Test of drug efficacy

1. Aphids are collected and raised indoor with 3-generation nymphs in tobacco seedlings.

2. Various formulated insecticides and controls were prepared from table one.

3. Test method

A plant-soaking inoculation method is adopted, test solution with corresponding concentration is prepared according to an embodiment and a comparison example, tobacco leaves are selected to be soaked in the test solution for 10 seconds, then the tobacco leaves are taken out and naturally dried, a cultured myzus persicae sensitive population is transferred to in-vitro tobacco leaves, then the tobacco leaves are placed in a culture dish, the tobacco leaves are cultured under indoor conditions, each concentration is repeated for 3 times, the tobacco leaves are placed in an observation room, then the number of dead and live insects is checked and recorded after 72 hours, and the death rate is calculated.

4. Investigation method

And (3) checking and recording the death condition of the test insects after 72 hours, wherein the judgment standard of the death of the test insects is as follows: death was counted as the absence of reaction when the test insects were gently touched by tweezers.

TABLE 1 test results

Insecticide	Formulation (concentration unit g/kg)	Myzus persicae corrected mortality (%)
			Example 1	Nicotine concentration 0.1, allicin concentration 0.27	18.16
Comparative example A1	Nicotine concentration 0.20	15.85
			Comparative example A2	Allicin concentration 0.54	16.28
Example 2	Nicotine concentration 0.15, allicin concentration 0.375	35.71
			Comparative example B1	Nicotine concentration 0.3	28.32
Comparative example B2	Allicin concentration 0.75	27.69
			Example 3	Nicotine concentration 0.25, allicin concentration 0.51	43.26
Comparative example C1	Nicotine concentration 0.5	37.32
			Comparative example C2	Allicin concentration 1.02	37.59
Example 4	Nicotine concentration 0.3, allicin concentration 0.60	68.39
			Comparative example D1	Nicotine concentration 0.60	50.32
Comparative example D2	Allicin concentration 1.20	49.87
			Example 5	Nicotine concentration 0.4, allicin concentration 0.84	75.30
Comparative example E1	Nicotine concentration 0.8	57.22
			Comparative example E2	Allicin concentration is 1.68	56.95

As can be seen from Table 1, the nicotine and the allicin have synergistic effect after being compounded, and when the concentration of the nicotine is more than or equal to 0.3g/kg, the concentration of the allicin is more than or equal to 0.60g/kg, the weight ratio of the nicotine to the allicin is 50: when the pesticide is 100 hours, the synergistic insecticidal effect is better and is obviously better than other embodiments.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. The compound pesticide is prepared by mixing and compounding nicotine and allicin, wherein the concentration of the nicotine is 0.40g/kg, the concentration of the allicin is 0.84g/kg, and the compound pesticide is a suspending agent, a wettable powder, a water dispersible granule or a microemulsion.