CN109221108A - A kind of stifling composition and its application in prevention and treatment straw mushroom pest - Google Patents
A kind of stifling composition and its application in prevention and treatment straw mushroom pest Download PDFInfo
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- CN109221108A CN109221108A CN201811056870.5A CN201811056870A CN109221108A CN 109221108 A CN109221108 A CN 109221108A CN 201811056870 A CN201811056870 A CN 201811056870A CN 109221108 A CN109221108 A CN 109221108A
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- straw mushroom
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- stifling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
Abstract
The invention discloses a kind of stifling composition and its applications in prevention and treatment straw mushroom pest.The stifling composition of prevention and treatment straw mushroom pest of the invention, based on parts by volume, including 1-2 parts of laurenes and 1 part of australene；The stifling composition has significantly stifling smoked kill activity for the straw mushroom pest in enclosed environment, can prevent and mitigate harm of the pest to straw mushroom mycelia.Stifling composition of the invention belongs to plant insecticide, easily decomposes, free from environmental pollution, to safety of human and livestock；Stifling composition loses activity after deinsectization, will not be accumulated in pest body or remain in food, protect ecological environment and food safety; preparation method is simple and easy, and raw material sources are wide, at low cost; it is suitble to large-scale production, can be widely used for the prevention and treatment of straw mushroom pest.
Description
Technical field
The invention belongs to biological pesticide technical fields, and in particular to a kind of stifling composition and its in prevention and treatment straw mushroom pest
Application.
Background technique
Currently, In Guangzhou Area mostly uses 6m × 4m × 3.2m specification standard mushroom house, tool statistics mushroom house is up between more than 6,000, day
25~30t of equal fresh grass mushroom landings.Straw mushroom product mainly based on marketing fresh, product except in addition to Guangzhou and Hongkong and Macro, Pearl River Delta sale,
There are also partially sell to the ground such as East China, Central China and North China.With the adjustment of strategy of agricultural development and production structure, straw mushroom has become
Developing rural economy, increase farmers' income a specialty industries project, it is maximum that In Guangzhou Area has become Guangdong Province or even the whole nation
Straw mushroom production base (Cao Yuhan, 2006).Straw mushroom primary pest has mushroom fly, mushroom cecidomyiia, melaena mosquito and mite etc., due to pest
Individual is small, concealment is strong, breeding is fast, and insect pest is difficult to control once breaking out.Therefore, should using aim at prevention, integrated control
Principle carries out integrated pest management (Liu Gui clear etc., 2008).By carrying out background survey discovery, the small telenomus of Shuan Eyan to straw mushroom
Bifrosina biform (Stenhammar) is the sociales of Guangzhou straw mushroom mushroom flies group, which is under the jurisdiction of Diptera
The small telenomus subfamily Limosininae in the natural pond Diptera Sphaeroceridae Sphaeroce-ridae, adult often in stack retting phase compost and
It creeps, mating, oviposition, has a significant effect when occurring greatly to straw mushroom yield, and easily lead to it on growth period of hypha and fructification
The pollution of its pathogen, causes plant disease epidemic, brings more huge economic loss.
The method for preventing and treating straw mushroom mushroom fly mainly includes physico mechanical control method and chemical prevention method.Physico mechanical control, i.e.,
The method for carrying out control of insect using the life habit of pest, mainly kills target pest using the method for physics, such as lures worm
Lamp etc..The shortcomings that this control method is that the quantity of trap lamp trapping is limited after all for the mushroom fly quantity in mushroom house,
The power grid of trap lamp fails because the close and numerous tiny corpse of mushroom fly has been paved with soon, and mushroom house hot humid environment
Short circuit is easily caused to burn trap lamp and bring security risk.In prevention practice, the frequent replacement of trap lamp also increases
Control cost is added.Chemical prevention method is exactly the method using the insect pest in the production of insecticide control straw mushroom.Chemical prevention method is most
Big advantage is that insecticidal effect is rapid, and thoroughly, insecticidal spectrum is wide, and processing cost is cheap, saves labor.The shortcomings that chemical prevention method is to kill
Worm agent is all toxic to people and higher mammal, commonly using can due to pest resistance to insecticide generation and have to improve using agent
Amount, is easy to bring different degrees of residual contamination problem to straw mushroom.Even so, chemical prevention method is still current killing pest
Most economical effective means.Due to weak tendency possessed by chemical pesticide itself and long-term unreasonable abuse, pest is resulted in
Resistance is generated to chemical pesticide, and polluted environment during chemical pesticide a large amount of uses, destroys the life of nature
State balance, compromises human health, has seriously affected our daily life.Therefore, botanical pesticide environmental protection increasingly
Today of attention receives the concern of world pesticide circle, and provides wide development sky for the development of environmentally friendly agricultural chemical
Between.Botanical pesticide is the substance with pesticide activity for being extracted from plants out, and active material can become innovation pesticide
Effective parent, further develop as new pesticide.Botanical pesticide compared with chemical pesticide, easily divided by effective component
Solution, is not easy to accumulate in pest body, is not easy to cause damages to other beneficial insects or natural enemy, can provide preserving the ecological environment
Beneficial to help.
Laurene (Myrcene, Chinese nickname: 7- methyl -3- methylene -1,6- octadiene；Myrcene) it is also known as myrcene,
Laurene is colourless or weak yellow liquid, has light face cream fragrance.It is not soluble in water, it is dissolved in the organic solvents such as ethyl alcohol.Come
Derived from plant, there is presence in cinnamon oil, lemon-grass oil, cedar wood oil, hemlock oil, turpentine oil, lemongrass oil, lemon oil etc..It can
Laurene is isolated with the method for vacuum fractionation.
Australene (α-Pinene), is a kind of important source material of synthetic perfume, and colourless transparent liquid is slightly soluble in water, insoluble
In propylene glycol, glycerol, it is dissolved in most organic solvents such as ethyl alcohol, ether, chloroform, glacial acetic acid.It is mainly used for synthesizing terpinol, fragrant camphor tree
Alcohol and some Sandalwood Odorants, there is the breath of pine, needle and resin-like.Can be used for chemical product and other industrial goods plus
It is fragrant.It is also the raw material of syntholube, plasticizer, terpene resin etc..
Summary of the invention
In view of the deficiencies of the prior art, it is an object of the present invention to provide a kind of stifling combinations that can effectively prevent straw mushroom pest
Object, have it is safe and non-toxic to humans and animals, it is environmentally friendly and the characteristics of to straw mushroom pest energy effectively preventing.
For inventor in the test of prevention and treatment straw mushroom pest, discovery laurene has stifling smoked kill for the mushroom fly in enclosed environment
Activity.Inventor through experiment it has also been found that, when australene is used alone for preventing and treating the small telenomus of the double volume rocks of straw mushroom pest, almost without
Smoked kill effect, but when australene and laurene are used in mixed way, then there is apparent synergistic effect to the latter.
Therefore, the first purpose of the invention is to provide a kind of stifling compositions for preventing and treating straw mushroom pest.
The stifling composition of the prevention and treatment straw mushroom pest, based on parts by volume, including 1-2 parts of laurenes and 1 part of australene.
It is preferred that the stifling composition of the prevention and treatment straw mushroom pest, based on parts by volume, including 1 part of laurene and 1 part of australene
Alkene.
The straw mushroom pest is the small telenomus Bifrosina biform (Stenhammar) of double volume rocks, food mycoceicidum mosquito
(Mycophila fungicola Felt) and/or melaena mosquito (Scatopse sp.).
The stifling composition of laurene and australene for preventing and treating straw mushroom pest of the invention can also be with some abilities
The well known chemical substance with the other plant volatile oils or other types of killing straw mushroom pest activity of field technique personnel carries out
Compounding.
Laurene and australene are as a kind of natural insecticide active substance, its activity disappears or by microorganism after use
It decomposes, does not pollute straw mushroom and environment, to safety of human and livestock, may be directly applied to the prevention and treatment of straw mushroom pest；Compared with chemical pesticide, the moon
Osmanthus alkene and australene are easily decomposed, and will not accumulate in pest body, therefore are not easy to other beneficial insects or pest natural enemy
It causes damages, protects ecological environment.
Laurene of the invention derives from perfume industry, in cinnamon oil, lemon-grass oil, cedar wood oil, hemlock oil, turpentine oil, lemon
There is presence in lemon grass oil, lemon oil etc..Laurene can be isolated with the method for vacuum fractionation.Required medicament source is wide, cost
It is low, be conducive to mass production, be convenient for large-scale straw mushroom control of insect application.Australene is a kind of important original of synthetic perfume
Material is mainly used for synthesizing terpinol, linalool and some Sandalwood Odorants, there is the breath of pine, needle and resin-like.It can use
In chemical product and the flavoring of other industrial goods.It is also the raw material of syntholube, plasticizer, terpene resin etc..
A second object of the present invention is to provide the stifling compositions of the prevention and treatment straw mushroom pest in prevention and treatment straw mushroom pest
In application.
The straw mushroom pest is the small telenomus Bifrosina biform (Stenhammar) of double volume rocks, food mycoceicidum mosquito
(Mycophila fungicola Felt) and/or melaena mosquito (Scatopse sp.).
It is preferred that the application, comprising the following steps: be suspended on the hygroscopic materials for being soaked with the stifling composition
It is fumigated in straw mushroom house.
The hygroscopic materials can for it is any can absorb liquid, quick volatilised liq and not with laurene, australene
The material chemically reacted.
It is preferred that the hygroscopic materials are filter paper.
The dosage of the stifling composition is that can be each standard room (6m X 4m X 3.2m) using 5mL-30mL,
Composition is fumigated using 10mL-30mL in preferably each 6m X 4m X 3.2m size criteria room.
The hygroscopic materials can be muti-piece, and the length of hygroscopic materials can be 30-40cm, preferably 35cm；It is wide
Degree can be 25-35cm, preferably 30cm；Thickness can be 0.4-0.6mm, preferably 0.53mm.When the hygroscopic materials
When specification is long 35cm × wide 30cm × thickness 0.53mm filter paper, every piece of filter paper can adsorb the medical fluid of about 10mL.
When the filter paper be double-round board qualitative filter paper, model: when [diameter 11cm (Φ 11cm), middling speed], every piece of filter paper
The medical fluid of 1mL can be adsorbed, each standard room (6m X 4m X 3.2m) needs the filter paper of the above-mentioned specification of 5-30 block.Note can be used
Emitter or suction pipe inject uniformly stifling composition on every piece of filter paper；When actually carrying out stifling smoked kill operation, can be used
Clip clamps the edge of filter paper, then ties down clip with cord or iron wire, so that the filter paper for being soaked with stifling composition be hung
Mushroom house is fumigated in mushroom house.
It is preferred that the described hygroscopic materials for being soaked with stifling composition are suspended in straw mushroom house carries out the stifling time
At least 72h, preferably 72-120h.
The suspension height of the hygroscopic materials for being soaked with stifling composition can be more excellent for apart from ground 18-32cm
It is 20-30cm；Select such suspension height that composition can be made more rapidly, equably to evaporate into mushroom house confined space,
Reach effective activity, thus the harmful organisms such as mushroom fly in smoked kill mushroom house.
Beneficial effects of the present invention: of the invention studies have shown that the stifling composition containing laurene and australene is for envelope
Straw mushroom pest in closed loop border has significantly stifling smoked kill activity, can prevent and mitigate harm of the pest to straw mushroom mycelia.
In conclusion stifling composition of the invention belongs to plant insecticide, easily decompose, it is free from environmental pollution, to people and animals
Safety；It loses activity, will not be accumulated in pest body or remains in food after the stifling composition deinsectization, protect life
State environment and food safety, preparation method is simple and easy, and raw material sources are wide, at low cost, is suitble to large-scale production, can use extensively
In the prevention and treatment of straw mushroom pest.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1
1.1 selected insect source
Double small telenomus of volume rock pick up from the female Volvaria volvacea cultivation field in Baiyun District Renhe Town east, choose bouncing adult and suck triangular flask
In take back laboratory, raising is in equipped with 300g straw mushroom compost, (waste cotton spends 55%, straw 40%, quick lime 5%；Equivalent siccative
In plastic casing (17cm × 12cm × 5cm) 100g), the gauze (30cm × 30cm × 30cm) of 100 mesh of plastic casing is covered, and is put
The heat and moisture preserving in the intelligent growth cabinet (manufacture of Ningbo Jiangnan instrument plant) of RXZ type (multistage programming), temperature is maintained at 22~
25 DEG C, humidity is 80% or more.After 3 generations of continuous raising, it is spare to form stable laboratory population.
1.2 test for toxicity of the examination composition to the small telenomus adult of double volume rocks
Using stifling composition of eclosion rate method (Liu Gui clear etc., the 2012) measurement containing laurene indoors to double small excrement of volume rock
The activity of fly adult.Plastic casing (17cm × 12cm × 5cm) fills 300g straw mushroom compost (equivalent siccative 100g), chooses 60 left sides
Right aging pupa is placed wherein, and plastic casing is placed in 100 mesh gauze insect cages (25cm × 25cm × 25cm), smoked kill test
When, double small telenomus quantity of volume rock are no less than 40 in cylinder mould.By it is each processing be individually placed in transparent chest (35cm × 35cm ×
In 35cm), 1 qualitative filter paper (model: diameter 11cm, middling speed) is hung in transparent chest, draws 1mL medicament drop on filter paper.
1mL clear water is added dropwise in blank control.Dead double small telenomus of volume rock are counted after 1d, 3d, 5d, 7d into borer population.Each processing, 3 repetitions.
It is analyzed with softwares such as statistical analysis system (SAS), data processing systems (DPS), and is mentioned referring to Mansour etc.
The total malicious factor (Co-toxicity factor) method out, the mixed of composition pesticide is judged with collaboration toxicity index (c.f)
Virulence, specific method are: setting confection as m, each single dose for forming m is a, b.It cooperates with toxicity index (c.f), mixture is theoretical dead
Dying rate is Pm.Measuring two kinds of mixed death rates of medicament is actual (Pc), while measuring both single doses in list
The actual (Pa, Pb) of used time.Wherein a the effective elements of the medicine percentage in Pa=a prescription used time actual × mixture
It counts, b the effective elements of the medicine percentage in Pb=b prescription used time actual × mixture.
Whether calculating the theoretical death rate (Pm) according to following equation, and differentiate synergy:
Mixture theoretical death rate (Pm)=1- (1-Pa) (1-Pb)
Cooperate with toxicity index (c.f)=(the actual measurement death rate-theoretical death rate)/theoretical death rate × 100
The insect population death rate (%)=(insect population number after insect population number-prevention and treatment before preventing and treating)/insect population number × 100 before preventing and treating
Corrected mortality (%)=(the medicine post processing zone insect population death rate-check plot insect population change rate)/(check plot 1- insect population
Change rate) × 100
Carry out the synergy of two kinds of pharmacy mix of qualitative evaluation with c.f value size, be wherein synergistic effect when c.f >=20,
It is antagonism when c.f≤- 20, is summation action when -20 < c.f > 20.
The results are shown in Table 1 for it.
Synergistic action effect of the different ratio to the small telenomus smoked kill of double volume rocks between 1 laurene of table and australene
Note: data are 3 duplicate average values in table.Carry out the joint of two kinds of pharmacy mix of qualitative evaluation with c.f value size
Effect is wherein synergistic effect when c.f >=20, is antagonism when c.f≤- 20, and when -20 < c.f > 20 is summation action.
As it can be seen from table 1 the collaboration toxicity index relative to single fumigant is applied in combination in laurene and australene
(c.f) in value 1d, 3d, 5d, 7d, there are 2 proportions 4 time points to be all larger than 20, show as synergistic effect, illustrate laurene and
Effect with synergy between australene, the stifling composition of the two, which has the double small telenomus of volume rock of straw mushroom primary pest, to be increased
Effect effect, the best proportion of synergistic effect is laurene: australene mixes (stifling composition 1), association with volume ratio 0.50:0.50
Same toxicity index (c.f) value 1d, 3d, 5d, 7d are respectively 216.52,159.24,68.49,54.69；Followed by laurene: australene
Alkene mixes (stifling composition 2) with volume ratio 0.66:0.33, and collaboration toxicity index (c.f) value 1d, 3d, 5d, 7d are respectively
83.77,27.57,37.16,23.76.Test result be also shown that the combined 1d, 3d of 3 proportions of laurene and australene, 5d,
7d collaboration toxicity index (c.f) value is all larger than -20, i.e., mixed to show as antagonism without an example.
Embodiment 2
Stifling composition 1 (by laurene and australene with the volume ratio 1:1 mixing) drop for drawing 1mL is fixed in one piece of double-round board
Property filter paper (model: diameter 11cm, middling speed) on, each standard room (6m X 4m X 3.2m) hangs the filter paper of 5 pieces of above-mentioned specifications, i.e.,
Dosage is 5mL.Syringe or suction pipe can be used to inject uniformly stifling composition 1 on every piece of filter paper, folder can be used
Sub-folder lives the edge of filter paper, then ties down clip with cord or iron wire, and filter paper suspension height is apart from ground 20cm, thus will
The filter paper for being soaked with stifling composition 1, which is suspended in mushroom house, fumigates mushroom house.
Embodiment 3
Stifling composition 1 (by laurene and australene with the volume ratio 1:1 mixing) drop for drawing 1mL is fixed in one piece of double-round board
Property filter paper (model: diameter 11cm, middling speed) on, each standard room (6m X 4m X 3.2m) hangs the filter paper of 10 pieces of above-mentioned specifications,
I.e. dosage is 10mL.Syringe or suction pipe can be used to inject uniformly stifling composition 1 on every piece of filter paper, can be used
Clip clamps the edge of filter paper, then ties down clip with cord or iron wire, filter paper suspension height be apart from ground 30cm, thus
The filter paper for being soaked with stifling composition 1 is suspended in mushroom house, mushroom house is fumigated.
Embodiment 4
Stifling composition 1 (by laurene and australene with the volume ratio 1:1 mixing) drop for drawing 1mL is fixed in one piece of double-round board
Property filter paper (model: diameter 11cm, middling speed) on, each standard room (6m X 4m X 3.2m) hangs the filter paper of 30 pieces of above-mentioned specifications,
I.e. dosage is 30mL.Syringe or suction pipe can be used to inject uniformly stifling composition 1 on every piece of filter paper, can be used
Clip clamps the edge of filter paper, then ties down clip with cord or iron wire, filter paper suspension height be apart from ground 30cm, thus
The filter paper for being soaked with stifling composition 1 is suspended in mushroom house, mushroom house is fumigated.
Test is carried out in certain Volvaria volvacea cultivation field, and mushroom house temperature and humidity is consistent with Routine Management during test.In embodiment 2-4
The mushroom house for choosing just inoculation strain, if blank control, each processing, 3 repetitions.Window, door and room middle in mushroom house set 3
A hitch point hangs fly catching paper, and suspension point height is 1.5m, and the suspension time is 0.5h, is inoculated with l d, 3d, 5d, 7d tune in strain
It looks into generation pest species and worm amount, control time is morning 11:00-12:00.Then count in mushroom house that adhere to straw mushroom each for fly catching paper
The quantity of kind class pest.Calculation formula is as follows:
Control efficiency (%)=(blank control borer population-corresponding embodiment borer population)/blank control borer population * 100
To the smoked killing effect of straw mushroom primary pest in 2 embodiment 2-4 of table
Note: data are 3 duplicate average values in table.
Fumigation activity experiments have shown that, processing 72h (3d) after, 10mL fumigate 1/ standard room of composition (6m X 4m X 3.2m) use
The stifling composition 1 for measuring (embodiment 3) reaches the control efficiency of the small telenomus of the double volume rocks of straw mushroom pest, melaena fly and food mycoceicidum mosquito
To after 57.50% or more, 120h (5d), control efficiency reaches high point.In embodiment 4,30mL fumigates 1/ standard room (6m of composition
X 4m X 3.2m) dosage control efficiency it is more preferable, reach in 3d to the small telenomus of the double volume rocks of straw mushroom pest, melaena fly and food bacterium
It is even more to reach 80.82% or more that the control efficiency of cecidomyiia reaches 73.78% or more, 120h (5d) afterwards.And straw mushroom production practices
In, 5d mycelia covers with culture medium after strain inoculation, and starts balling.In mycelia growth course, a large amount of pest is in culture medium
On creep, broken mycelia so that the mycelia of balling is reduced, the straw mushroom underproduction.And after mycelia a large amount of ballings, pest also just disappears
?.Therefore, effective pest controling effect can be played in the material time section that straw mushroom produces using stifling 1 smoked kill of composition,
It is of practical significance in straw mushroom production.
Claims (10)
1. a kind of stifling composition for preventing and treating straw mushroom pest, which is characterized in that based on parts by volume, including 1-2 parts of laurenes and 1 part
Australene.
2. the stifling composition of prevention and treatment straw mushroom pest according to claim 1, which is characterized in that based on parts by volume, including 1
Part laurene and 1 part of australene.
3. the stifling composition of prevention and treatment straw mushroom pest according to claim 1, which is characterized in that the straw mushroom pest is
The small telenomus of double volume rocks, food mycoceicidum mosquito and/or melaena mosquito.
4. application of the stifling composition of prevention and treatment straw mushroom pest described in claim 1 in prevention and treatment straw mushroom pest.
5. application according to claim 4, which is characterized in that the straw mushroom pest is the small telenomus of double volume rocks, food mycoceicidum
Mosquito and/or melaena mosquito.
6. application according to claim 4, which comprises the following steps: will be soaked with described in claim 1 smoked
The hygroscopic materials for steaming composition, which are suspended in straw mushroom house, to be fumigated.
7. application according to claim 6, which is characterized in that the hygroscopic materials are filter paper.
8. application according to claim 6, which is characterized in that the dosage of the stifling composition is each 6m X 4m
Composition is fumigated using 10mL-30mL in X 3.2m size criteria room.
9. application according to claim 6, which is characterized in that the fumigation time is at least 72h.
10. application according to claim 6, which is characterized in that described is soaked with stifling composition described in claim 1
Hygroscopic materials suspension height be apart from ground 20-30cm.
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| CN111052980A (en) * | 2019-12-19 | 2020-04-24 | 广东省生物资源应用研究所 | Method for preventing and treating straw mushroom pests by electrotherapy |
| CN111838152A (en) * | 2020-06-19 | 2020-10-30 | 中国医学科学院药用植物研究所 | Lycium barbarum gallmidge attractant composition, lure and application |
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| CN108244111A (en) * | 2018-03-02 | 2018-07-06 | 广西壮族自治区农业科学院植物保护研究所 | Combine volatile compound and its application after biocontrol of mango is adopted in anthracnose |
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| CN108244111A (en) * | 2018-03-02 | 2018-07-06 | 广西壮族自治区农业科学院植物保护研究所 | Combine volatile compound and its application after biocontrol of mango is adopted in anthracnose |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111052980A (en) * | 2019-12-19 | 2020-04-24 | 广东省生物资源应用研究所 | Method for preventing and treating straw mushroom pests by electrotherapy |
| CN111838152A (en) * | 2020-06-19 | 2020-10-30 | 中国医学科学院药用植物研究所 | Lycium barbarum gallmidge attractant composition, lure and application |
| CN111838152B (en) * | 2020-06-19 | 2021-06-29 | 中国医学科学院药用植物研究所 | Lycium barbarum gallmidge attractant composition, lure and application |
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