CN116195589A - Repellent for bark beetle adults in spruce and application thereof - Google Patents
Repellent for bark beetle adults in spruce and application thereof Download PDFInfo
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- CN116195589A CN116195589A CN202210696569.0A CN202210696569A CN116195589A CN 116195589 A CN116195589 A CN 116195589A CN 202210696569 A CN202210696569 A CN 202210696569A CN 116195589 A CN116195589 A CN 116195589A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P17/00—Pest repellants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A repellent for bark beetle adults in spruce and application thereof belong to the technical field of pest repellent by utilizing chemical information substances. The repellent for the bark beetle adults of the spruce comprises the following components in parts by weight: 1-100 parts of 1, 8-eucalyptol and 1-100 parts of 3-carene. The bark beetle imago repellent can be hung around the trunk part of a bark beetle host tree for repelling the damage of bark beetle imago boring trees. The repellent has the lasting period of 30-120 days, is environment-friendly, reduces the use of chemical pesticides, is particularly suitable for popularization and application in forests, and has good economic, social and ecological benefits and wide industrialization prospect.
Description
Technical Field
The invention belongs to the technical field of pest expelling by using chemical information substances, and particularly relates to a repellent for bark beetle adults in spruce and application thereof.
Background
The spruce bark beetle Ips typographus Linnaeus belongs to Coleoptera, scolytidae of bark beetle, bark beetle Ips, the foreign original host is norway spruce (Picea abies (l.)) which is one of the most important economic tree species in europe, and is mainly harmful to red Pi Yunsha Picea koiensis, ichthyophthalmis var. The spruce bark beetles are widely distributed in the whole ancient north area, are important trunk-boring pests in the spruce ecosystem, and are secondary pests. Natural spruce forest is damaged, especially mature spruce forest may be damaged directly to healthy tree under proper condition or in serious pest damage. The severe harm of the bark beetles of the spruce, which causes the death of the forest in a large area, brings huge ecological and economic losses to the local forest ecological environment and the forest production, and is considered to be the most damaging species in the needle leaf forest so far.
Spruce relies on structural defense and induced defense to combat the invasive spruce bark beetle, the first line of defense against the spruce bark beetle being the bark that will contribute to their long-term survival by producing large amounts of defense chemicals to combat the invasive spruce bark that prevents the penetration of the spruce bark beetle by its own physical and chemical toxicity. Once injury or infection is recognized by spruce, induction of defenses including traumatic resin ducts and the formation of polyphenol parenchyma cells is stimulated, while terpene concentrations may be increased 100-fold or more, with increasing concentrations, more effective inhibition of the colonization of spruce octopus. It was found that trans-4-biotanol (trans-4-thujanol) decreased in bark with age, and this material was also detected in spruce volatiles that were male-drilled by the spruce bark beetle. During the spruce bark beetle outbreak, host trees that were not challenged with spruce bark beetles released a greater amount of 1,8-cineole than successfully colonized host trees. Under laboratory conditions, both trans-4-thujanol and 1,8-cineole were demonstrated to inhibit the aggregation pheromone of the bark beetle in spruce.
Non-host green leaf volatiles can interfere with the response of the bark beetle to pheromones. 3 major components of birch: l-hexanol (l-hexanoal), (Z) -3-hexen-l-ol) and (E) -2-hexen-l-ol can cause strong antennary potential reaction of spruce octopus vulgaris, and the trapping amount can be reduced by adding the 3 substances into artificially synthesized pheromone; cis-2-hexenol (cis-2-Hexen-l-ol) with a volume concentration of 0.001, trans-2-hexenol (trans-2-Hexen-1-ol), cis-3-hexenol (cis-3-Hexen-1-ol) and 3-Carene (3-Carene) are mixed, trans-2-hexenal (trans-2-hexenal) with a volume concentration of 0.01, and (-) -isocaryophyllene (isocaryophyllene) and 3-Carene are mixed, and benzaldehyde (benzaldehyde) with a volume concentration of 0.001 or 0.01 has a repellent effect on picea octapinnatifida when mixed with 3-Carene.
However, the repellents in the prior art all have multicomponent compositions, have poor repellent effects, and are based on laboratory conditions, and are reported in forests.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to design and provide a repellent for the bark beetle adults of spruce and a technical scheme for application in forests.
The invention is realized by the following technical scheme:
the invention provides a repellent for bark beetle adults in spruce, which comprises the following components in parts by volume:
1-100 parts of 1, 8-eucalyptol
1-100 parts of 3-carene.
Further, the repellent for the bark beetle adults of the spruce comprises the following components in parts by volume:
5-50 parts of 1, 8-eucalyptol
5-50 parts of 3-carene.
Further, the repellent for the bark beetle adults of the spruce comprises the following components in parts by volume:
10-30 parts of 1, 8-eucalyptol
10-30 parts of 3-carene.
Further, the volume ratio of the 1, 8-eucalyptol to the 3-carene is 1:0.1-10.
Further, the volume ratio of 1, 8-eucalyptol to 3-carene is 1:1.
The invention further provides an application of the repellent for the bark beetle adults in spruce as the repellent for the bark beetle adults in spruce.
The preparation process of the bark beetle imago repellent for spruce comprises the following specific steps:
1) Preparation of a slow release bag: soaking the degradable slow release bag in ethanol for 1-3 days, washing with distilled water until no ethanol smell exists, and air drying for use;
2) Preparation of repellent: the parts of the active ingredients in the formula are respectively and accurately weighed in milliliters for later use;
3) And (3) adding absorbent cotton or gauze and other mediums into the slow release bag, and respectively dripping the repellent prepared in the step (2) into the lure carrier prepared in the step (1), thereby preparing the repellent for the bark beetle adults in spruce.
The bark beetle imago repellent can be hung around the trunk part of a bark beetle host tree for repelling the damage of bark beetle imago boring trees. The repellent has the lasting period of 30-120 days, is environment-friendly, reduces the use of chemical pesticides, is particularly suitable for popularization and application in forests, and has good economic, social and ecological benefits and wide industrialization prospect.
Drawings
FIG. 1 is an attracting amount of the bark beetle adults of the spruce of each example.
Detailed Description
The invention is further illustrated by the following examples.
Example 1: preparation (volume portion) of spruce bark beetle repellent
1 part of 1, 8-eucalyptol and 1 part of 3-carene are weighed and respectively put into a slow-release bag filled with absorbent cotton, thus obtaining the repellent for the bark beetle adults on spruce and octopus.
Example 2: preparation (volume portion) of spruce bark beetle repellent
Weighing 5 parts of 1, 8-eucalyptol and 5 parts of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the repellent for the bark beetle adults.
Example 3: preparation (volume portion) of spruce bark beetle repellent
Weighing 10 parts of 1, 8-eucalyptol and 10 parts of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the repellent for the bark beetle adults.
Example 4: preparation (volume portion) of spruce bark beetle repellent
Weighing 100 parts of 1, 8-eucalyptol and 100 parts of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the repellent for the bark beetle adults.
Example 5: preparation (volume portion) of spruce bark beetle repellent
Weighing 10 parts of 1, 8-eucalyptol and 1 part of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the repellent for the bark beetle adults.
Example 6: preparation (volume portion) of spruce bark beetle repellent
Weighing 5 parts of 1, 8-eucalyptol and 1 part of 3-carene, and respectively putting into a slow-release bag filled with absorbent cotton to obtain the repellent for the bark beetle adults.
Example 7: preparation (volume portion) of spruce bark beetle repellent
1 part of 1, 8-eucalyptol and 5 parts of 3-carene are weighed and respectively put into a slow-release bag filled with absorbent cotton, thus obtaining the repellent for the bark beetle adults on spruce and octopus.
Comparative example 1
1 part of 1, 8-eucalyptol is weighed and put into a slow-release bag filled with absorbent cotton, and the repellent for the bark beetle adults on spruce is prepared.
Comparative example 2
Weighing 5 parts of 3-carene, and putting into a slow-release bag filled with absorbent cotton to obtain the repellent for the bark beetle adults.
Comparative example 3
And weighing 4 g of myrcene, and putting the myrcene into a slow-release bag filled with absorbent cotton to obtain the repellent for the bark beetle adults.
Comparative example 4
4 g of nonanal is weighed and put into a slow-release bag filled with absorbent cotton, and the repellent for the bark beetle adults in spruce is prepared.
Test example 1: the spruce bark beetle repellent prepared in examples 1, 2, 3 and 5 and comparative examples 1, 2, 3 and 4 was uniformly mixed with an attractant (60 parts of cis-verbenol, 60 parts of 2-methyl-3-butene-2-ol, 60 parts of alpha-pinene (+ 25/-75), 0.02 part of 2, 6-di-tert-butyl-p-cresol and 0.02 part of 2-hydroxy-4-methoxybenzophenone) respectively, and the mixture was precisely weighed in milliliters for later use, and the attractant was an existing product, and the preparation method thereof was specifically and visually suspended at a connecting rod of a funnel-type trap in China patent publication No. CN105746511A and 201610183543.0. The traps were hung in the Wangqing forestry bureau Jingouling forest farm 36 Lin Ban with a trap spacing of 100m, and each treatment and repetition were randomly distributed therein. The height of the trapper hung between two trees is about 2m, the distance between the lower hem of the trapper and the ground is about 50cm, and the hanging time is from the middle ten days of 4 months to the beginning of 8 months. Each treatment was repeated 5 times. The data analysis adopts a single-factor analysis of variance method, and Duncan complex polar error test is adopted.
As can be seen from the survey data in table 1 and fig. 1, the repellent according to the present invention can significantly reduce the trapping effect of the attractants.
Table 1: trapping effect of various embodiments of Wangqing forestry bureau in Jilin province of 2020
| Different embodiments | Trapping quantity + -standard error | Difference significance |
| Attractant of existing patent formula | 4611.4±660.17 | a |
| Comparative example 2 | 4499.2±761.32 | a |
| Comparative example 3 | 1874.8±446.56 | b |
| Comparative example 4 | 1542.2±126.69 | b |
| Comparative example 1 | 1528.8±67.3 | b |
| Example 1 | 356.8±156.71 | c |
| Example 2 | 297.2±50.41 | c |
| Example 3 | 171.8±21.73 | c |
| Example 5 | 285±71.85 | c |
| Blank control | 0.4±0.24 | c |
Note that: the different letters represent a significant difference at the 0.05 level.
Test example 2: 50-head picea spruce bark beetles collected in the field are taken out of the picea spruce bark beetle repellent prepared in the embodiment 4, fed for 5 days indoors, starved for 3 hours before the test, and tested by an indoor Y-type olfactometer, and the results are shown in Table 2. The test results show that: the repellent effect of the lure formula is extremely higher than that of a blank control.
Table 2 repellent effect of the present invention (Y type olfactometer)
χ 2 -test:*P<0.05；**P<0.005.
Test example 3: 50-head picea spruce bark beetles collected in the field are taken out of the picea spruce bark beetle repellent prepared in the embodiment 6, fed for 5 days indoors, starved for 3 hours before the test, and tested by an indoor Y-type olfactometer, and the results are shown in Table 3. The test results show that: the repellent effect of the lure formula is extremely higher than that of a blank control.
Table 3 comparison of repellent effects of the present invention (Y-type olfactometer)
χ 2 -test:*P<0.05；**P<0.005.
Test example 4: 50-head picea spruce bark beetles collected in the field are taken out of the picea spruce bark beetle repellent prepared in the embodiment 7, fed for 5 days indoors, starved for 3 hours before the test, and tested by an indoor Y-type olfactometer, and the results are shown in Table 3. The test results show that: the repellent effect of the lure formula is extremely higher than that of a blank control.
Table 4 comparison of repellent effects of the present invention (Y-type olfactometer)
χ 2 -test:*P<0.05；**P<0.005.
Test example 5: 50-head picea spruce bark beetles collected in the field are taken out of the picea spruce bark beetle repellent prepared in the embodiment 5, fed for 5 days indoors, starved for 3 hours before the test, and tested by an indoor Y-type olfactometer, and the results are shown in Table 3. The test results show that: the repellent effect of the lure formula is extremely higher than that of a blank control.
Table 5 comparison of repellent effects of the present invention (Y-type olfactometer)
χ 2 -test:*P<0.05；**P<0.005。
Claims (6)
1. The repellent for the bark beetle adults of spruce is characterized by comprising the following components in parts by volume:
1-100 parts of 1, 8-eucalyptol
1-100 parts of 3-carene.
2. The repellent for the adult bark beetles of spruce as claimed in claim 1, comprising the following components in parts by volume:
5-50 parts of 1, 8-eucalyptol
5-50 parts of 3-carene.
3. The repellent for the adult bark beetles of spruce as claimed in claim 1, comprising the following components in parts by volume:
10-30 parts of 1, 8-eucalyptol
10-30 parts of 3-carene.
4. A repellent for bark beetle adults in spruce according to any one of claims 1 to 3, characterized in that the volume ratio of 1, 8-eucalyptol to 3-carene is 1:0.1-10.
5. A repellent for the adults of the bark beetle of spruce, according to any one of claims 1 to 3, characterized in that the volume ratio of 1, 8-eucalyptol to 3-carene is 1:1.
6. Use of a repellent for adults of the bark beetle of spruce according to any one of claims 1 to 3 as a repellent for adults of the bark beetle of spruce.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210696569.0A CN116195589A (en) | 2022-06-20 | 2022-06-20 | Repellent for bark beetle adults in spruce and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210696569.0A CN116195589A (en) | 2022-06-20 | 2022-06-20 | Repellent for bark beetle adults in spruce and application thereof |
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| CN116195589A true CN116195589A (en) | 2023-06-02 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114946846A (en) * | 2022-06-20 | 2022-08-30 | 吉林省林业科学研究院(吉林省林业生物防治中心站) | Method for treating ips typographus by applying push-pull strategy |
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2022
- 2022-06-20 CN CN202210696569.0A patent/CN116195589A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114946846A (en) * | 2022-06-20 | 2022-08-30 | 吉林省林业科学研究院(吉林省林业生物防治中心站) | Method for treating ips typographus by applying push-pull strategy |
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